Standard name
Human Ortholog
Description Ubiquitin-specific protease that cleaves ubiquitin-protein fusions; UBP9 has a paralog, UBP13, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0.08 0.05 0.11 0 0 0 0 0 – 0 0.06 0 0 0.05 0.11 0.14 0.1 0 0.06 0.09 0.1 0.1 0.11
Cytoplasm 0.98 0.97 0.99 0.97 1.0 0.98 0.97 0.97 0.91 – 0.88 0.91 0.99 0.99 1.0 0.89 0.92 0.88 0.8 0.76 0.7 0.6 0.61 0.58
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0.08 0.05 0.1 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0.1 – 0.21 0.11 0 0 0 0 0 0 0.06 0.06 0.08 0.11 0.11 0.08
Nucleus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 1 0 1 0 3 – 2 3 1 0 0 2 4 1 4 2 8 7 14 12
Bud 1 1 0 1 0 2 1 3 7 – 14 7 5 0 1 0 2 0 0 2 1 4 10 15
Bud Neck 1 2 10 2 13 11 21 18 15 – 4 17 0 0 1 1 3 3 3 6 6 7 12 11
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 11 16 26 7 15 14 8 15 – 11 26 8 15 23 24 21 12 7 8 28 18 33 38
Cytoplasm 207 137 326 222 395 445 630 412 412 – 368 411 349 382 457 187 136 101 177 112 212 116 209 207
Endoplasmic Reticulum 3 1 2 0 0 0 0 1 1 – 0 1 0 3 1 16 8 12 5 0 3 4 3 6
Endosome 0 0 0 0 0 0 0 0 0 – 0 0 0 1 0 2 0 2 0 1 0 1 2 3
Golgi 1 0 0 0 0 0 0 0 0 – 0 0 0 0 0 3 0 2 2 1 0 0 1 2
Mitochondria 2 2 0 2 0 8 27 4 45 – 89 48 3 2 0 7 0 2 12 9 25 20 37 27
Nucleus 0 0 0 0 0 0 0 3 0 – 2 1 0 0 0 0 0 1 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 1 0 0 0 – 0 0 0 0 1 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 – 0 1 0 0 0 0 1 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 1 0 0 0 0 0 0 0
SpindlePole 0 0 1 0 1 2 5 0 3 – 4 4 0 0 0 0 1 4 0 0 1 0 1 2
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 5 2 1 3 0 1 5 9 19
Unique Cell Count 211 141 329 228 396 452 648 425 452 416 452 351 386 459 209 148 115 221 147 302 196 342 358
Labelled Cell Count 215 154 355 253 417 484 699 449 501 494 519 366 403 484 247 179 141 221 147 302 196 342 358


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.8 4.0 4.5 3.9 4.7 3.6 3.9 4.1 3.4 – 3.3 3.8 4.0 4.3 4.3 6.8 6.5 6.5 4.0 4.0 4.5
Std Deviation (1e-4) 0.5 0.8 0.9 0.7 1.1 1.0 1.0 0.8 0.7 – 0.7 0.6 1.1 1.4 1.3 1.9 1.7 1.7 1.3 1.3 1.8
Intensity Change (Log2) – – – -0.2 0.05 -0.31 -0.21 -0.13 -0.41 – -0.46 -0.26 -0.16 -0.05 -0.06 0.59 0.54 0.54 -0.17 -0.18 -0.01


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -1.1705 -1.5223 -1.6478 -1.8207 -1.9303 -1.5161 -0.5435 -0.8659 -0.5484 -0.8398 -0.8724 -0.4699 -0.7538 -0.5999 -0.7209 -0.2495 -0.8202 -0.9754
Actin 0.0496 0.0021 0.0061 0.0357 0.0161 0.0121 0.0503 0.0022 0.0423 0.0073 0.0294 0.0209 0.0362 0.0023 0.0207 0.0449 0.001 0.0079
Bud 0.0006 0.0007 0.0004 0.0007 0.0001 0.0007 0.002 0.0008 0.0032 0.0006 0.0095 0.0012 0.0006 0.0001 0.0008 0.0006 0.0001 0.001
Bud Neck 0.0021 0.0003 0.0022 0.0003 0.0008 0.0014 0.0053 0.0014 0.001 0.0003 0.0015 0.0076 0.0005 0.0002 0.0009 0.0011 0.0002 0.0052
Bud Periphery 0.0009 0.0006 0.0006 0.0014 0.0002 0.002 0.0044 0.0006 0.0056 0.0016 0.0365 0.0035 0.0005 0.0001 0.0017 0.0011 0.0001 0.0022
Bud Site 0.0069 0.0155 0.0015 0.0025 0.0004 0.0006 0.0091 0.0166 0.0253 0.0004 0.0103 0.0031 0.0033 0.0029 0.0108 0.0034 0.0003 0.0025
Cell Periphery 0.0007 0.0011 0.0005 0.0002 0.0002 0.0003 0.0015 0.002 0.001 0.0004 0.0014 0.0008 0.0002 0.0004 0.0007 0.0004 0 0.0002
Cytoplasm 0.0338 0.0959 0.0682 0.0299 0.1007 0.0644 0.0839 0.216 0.1459 0.1171 0.1745 0.1642 0.027 0.0679 0.1229 0.0879 0.0429 0.1206
Cytoplasmic Foci 0.0335 0.0054 0.0146 0.0087 0.0117 0.0262 0.0417 0.0039 0.007 0.0185 0.0165 0.0204 0.0302 0.0042 0.0158 0.0163 0.0239 0.015
Eisosomes 0.0019 0.0006 0.0004 0.0007 0.0017 0.0004 0.0008 0.0006 0.0006 0.0004 0.0005 0.0003 0.0006 0.0002 0.0007 0.0007 0.0001 0.0002
Endoplasmic Reticulum 0.005 0.0037 0.0016 0.0021 0.0021 0.0017 0.0046 0.0028 0.0025 0.0015 0.0028 0.0013 0.0013 0.0004 0.0011 0.0013 0.0006 0.0004
Endosome 0.0306 0.001 0.0071 0.0113 0.0159 0.0066 0.029 0.0008 0.0077 0.0038 0.0462 0.0121 0.0137 0.0004 0.0052 0.0135 0.0191 0.0041
Golgi 0.0065 0.0006 0.0079 0.0126 0.0261 0.0034 0.0116 0.0002 0.0073 0.0036 0.0137 0.0132 0.0051 0.0008 0.0052 0.0158 0.0058 0.0031
Lipid Particles 0.0229 0.0009 0.0124 0.0128 0.0125 0.0057 0.0187 0.0003 0.0021 0.0277 0.0088 0.0163 0.0248 0.0007 0.0056 0.0164 0.0023 0.0062
Mitochondria 0.0027 0.0004 0.0141 0.0298 0.0189 0.0025 0.0087 0.0002 0.0195 0.0324 0.0138 0.0209 0.0022 0.0002 0.0046 0.0053 0.001 0.004
None 0.7187 0.8644 0.8377 0.842 0.7466 0.8574 0.6575 0.7485 0.7209 0.7734 0.5899 0.6944 0.8024 0.916 0.7881 0.7671 0.8942 0.8179
Nuclear Periphery 0.0118 0.001 0.0013 0.0028 0.0123 0.0049 0.01 0.0002 0.0017 0.0003 0.0012 0.0021 0.0067 0.0001 0.0007 0.0011 0.0005 0.0002
Nucleolus 0.0051 0.0008 0.0005 0.0003 0.002 0.0003 0.0016 0.0005 0.0003 0.0002 0.0003 0.0007 0.0022 0.0001 0.0004 0.0004 0.0001 0.0004
Nucleus 0.016 0.0009 0.0009 0.0007 0.002 0.0007 0.0214 0.0012 0.0008 0.0005 0.0007 0.0021 0.0025 0.0002 0.0004 0.0005 0.0002 0.0003
Peroxisomes 0.0138 0.0001 0.0182 0.0022 0.0204 0.0038 0.0203 0.0001 0.0026 0.0077 0.0347 0.0075 0.024 0.0023 0.0115 0.0192 0.0055 0.0066
Punctate Nuclear 0.0208 0.0028 0.003 0.0011 0.0076 0.0039 0.0086 0.0006 0.0009 0.0011 0.001 0.0052 0.0145 0.0004 0.0016 0.0018 0.0014 0.0017
Vacuole 0.0133 0.0009 0.0006 0.0011 0.0007 0.0004 0.0072 0.0006 0.0012 0.0011 0.0051 0.0013 0.0011 0.0003 0.0004 0.0007 0.0004 0.0003
Vacuole Periphery 0.0028 0.0001 0.0003 0.0012 0.001 0.0005 0.0019 0 0.0005 0.0002 0.0016 0.0008 0.0004 0 0.0002 0.0004 0.0001 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 4.1408 0.871 1.2926 5.1426 3.7897 4.8688 5.0637 7.1325 6.6705 6.6821
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency –

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
862 357 56 106 138 117 2650 180 1000 474 2706 286

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 646.40 741.76 963.88 848.80 661.61 720.53 813.42 874.98 648.50 736.52 816.53 865.28
Standard Deviation 62.39 89.33 101.44 117.06 73.32 84.93 95.16 88.13 64.23 88.74 97.67 100.63
Intensity Change Log 2 — 0.198525 0.576426 0.392997 — 0.123077 0.298019 0.403269 — 0.160856 0.442314 0.398202

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000660 0.000767 0.000638 0.001420 0.000472 0.000460 0.000140 0.000265 0.000634 0.000691 0.000150 0.000693
Bud Neck 0.025254 0.048318 0.001409 0.041588 0.033451 0.015875 0.008767 0.003474 0.026385 0.040310 0.008615 0.017600
Bud Site 0.004626 0.014073 0.004800 0.029302 0.018343 0.010849 0.010574 0.008169 0.006519 0.013277 0.010455 0.016001
Cell Periphery 0.000577 0.000276 0.000443 0.000648 0.000482 0.000474 0.000199 0.000216 0.000564 0.000325 0.000204 0.000376
Cytoplasm 0.537303* 0.351208* 0.423744* 0.122662* 0.389666* 0.399038* 0.455627* 0.342036* 0.516929* 0.363014* 0.454967* 0.260729*
Cytoplasmic Foci 0.216402* 0.342911* 0.069658 0.009582 0.310591* 0.304918* 0.009494 0.031716 0.229400* 0.333533* 0.010739 0.023512
Eisosomes 0.001198 0.000344 0.000171 0.000128 0.000719 0.000662 0.000027 0.000121 0.001132 0.000422 0.000030 0.000123
Endoplasmic Reticulum 0.000559 0.000423 0.004296 0.012731 0.000367 0.000455 0.000839 0.003504 0.000532 0.000431 0.000911 0.006924
Endosome 0.012017 0.034397 0.008168 0.010859 0.017138 0.017173 0.001531 0.012244 0.012724 0.030146 0.001668 0.011731
Golgi 0.002046 0.006293 0.000137 0.000996 0.001737 0.002764 0.000245 0.003085 0.002003 0.005422 0.000243 0.002311
Lipid Particles 0.010221 0.007367 0.004503 0.002519 0.007765 0.023147 0.000508 0.002366 0.009882 0.011262 0.000591 0.002423
Mitochondria 0.002781 0.005290 0.000252 0.011475 0.001001 0.001408 0.000843 0.003380 0.002535 0.004332 0.000831 0.006381
Mitotic Spindle 0.000070 0.001845 0.000219 0.022557 0.000069 0.000111 0.005783 0.030720 0.000070 0.001417 0.005667 0.027695
None 0.003093 0.001112 0.002250 0.002028 0.002463 0.000702 0.004472 0.002371 0.003006 0.001011 0.004426 0.002244
Nuclear Periphery 0.000242 0.000241 0.001110 0.001884 0.000210 0.000532 0.000810 0.001190 0.000237 0.000312 0.000816 0.001447
Nuclear Periphery Foci 0.000300 0.000404 0.004884 0.001582 0.000327 0.000474 0.000208 0.001318 0.000304 0.000421 0.000305 0.001416
Nucleolus 0.000642 0.000770 0.000335 0.003187 0.000625 0.000581 0.000508 0.000170 0.000639 0.000723 0.000505 0.001288
Nucleus 0.114031 0.077290 0.288058* 0.622417* 0.088327 0.143722* 0.470444* 0.277689* 0.110484 0.093688 0.466670* 0.405455*
Peroxisomes 0.004687 0.019331 0.000824 0.001947 0.017251 0.017799 0.000415 0.001154 0.006421 0.018953 0.000424 0.001448
Vacuole 0.062852 0.086292 0.183931 0.098373 0.108420 0.058478 0.028317 0.274512* 0.069141 0.079426 0.031537 0.209229*
Vacuole Periphery 0.000440 0.001049 0.000170 0.002116 0.000574 0.000380 0.000247 0.000302 0.000458 0.000884 0.000245 0.000974

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -0.19 -0.06 -1.03 -1.25 -1.56 0.05 2.25 0.93 0.43 -4.09 -0.11 1.18 -0.07 0.10 -4.60
Bud Neck -3.56 10.50 -0.91 1.10 -3.26 2.28 3.80 4.38 2.41 2.91 -2.64 7.86 2.35 3.95 -1.82
Bud Site -4.77 -0.25 -2.87 -1.22 -2.77 0.71 1.17 1.34 0.30 0.68 -2.55 -1.99 -2.46 0.01 -1.36
Cell Periphery 2.31 1.23 -0.25 -3.50 -1.92 0.05 3.09 2.95 4.29 -0.34 2.12 3.50 1.91 -0.46 -4.16
Cytoplasm 12.03 5.07 23.45 11.09 6.48 -0.26 -1.66 5.42 5.41 10.02 11.18 10.33 21.68 9.69 16.06
Cytoplasmic Foci -9.48 12.99 33.56 27.14 4.19 0.32 15.82 14.87 13.46 -4.76 -8.72 38.74 35.05 29.60 -3.61
Eisosomes 3.98 4.77 4.94 4.57 0.90 0.41 6.40 5.35 5.35 -11.49 3.81 5.99 5.38 6.99 -14.30
Endoplasmic Reticulum 1.17 -5.41 -7.82 -7.89 -4.26 -1.10 -8.00 -7.52 -6.05 -6.19 0.29 -6.46 -9.57 -9.60 -8.75
Endosome -6.05 2.58 1.94 6.62 -0.05 0.03 4.64 3.38 3.02 -1.42 -5.72 10.49 4.18 7.79 -2.79
Golgi -2.60 8.15 3.37 3.46 -1.30 -0.76 4.34 1.59 1.48 -0.79 -2.67 8.09 3.13 3.65 -1.11
Lipid Particles 2.15 4.76 7.87 4.69 1.95 -1.95 4.96 3.54 2.70 -4.52 -0.61 11.52 8.64 4.27 -6.50
Mitochondria -1.87 3.34 -1.04 -0.37 -1.79 -0.96 0.69 0.23 0.80 -0.07 -1.67 2.65 -0.27 0.83 -1.59
Mitotic Spindle -1.98 -1.43 -2.07 -1.85 -2.05 -0.52 -6.43 -1.77 -1.76 -0.54 -1.99 -6.43 -2.72 -2.39 -1.35
None 2.73 0.78 1.02 -1.74 -0.18 1.02 -0.90 -1.04 -1.96 -0.63 3.09 -1.57 -0.85 -2.08 -0.24
Nuclear Periphery -0.04 -7.52 -10.26 -10.09 -4.25 -2.78 -16.85 -10.64 -5.56 -4.32 -1.70 -19.75 -14.48 -12.83 -7.01
Nuclear Periphery Foci -1.27 -5.13 -4.14 -3.57 3.66 -0.68 0.71 -5.03 -2.75 -5.50 -1.45 -1.20 -6.79 -5.28 -6.19
Nucleolus -0.93 2.53 -1.83 -1.72 -2.07 0.27 0.71 1.70 2.16 1.58 -0.72 1.09 -1.34 -1.17 -1.63
Nucleus 5.14 -4.82 -15.61 -16.58 -6.69 -3.28 -32.43 -7.35 -4.00 12.39 2.21 -51.84 -13.57 -13.95 4.74
Peroxisomes -6.53 4.70 4.48 8.39 -0.64 -0.04 3.11 3.11 2.78 0.08 -5.14 6.25 5.88 8.20 -1.43
Vacuole -3.87 -6.10 -4.45 -3.08 2.91 3.09 4.16 -12.44 -15.41 -16.58 -2.07 2.67 -14.24 -13.38 -14.88
Vacuole Periphery -3.96 5.96 -1.62 -0.48 -2.15 1.29 2.42 3.02 3.23 1.47 -3.52 4.17 -0.51 1.36 -1.67
Standard name
Human Ortholog
Description Ubiquitin-specific protease that cleaves ubiquitin-protein fusions; UBP9 has a paralog, UBP13, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (34%), ER (1%), mixed (37%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Ubp9

Ubp9


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ubp9-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available