Standard name
Human Ortholog
Description Lipid raft associated protein; overexpression restores Pma1p localization to lipid rafts which is required for targeting of Pma1p to the plasma membrane; sometimes classified in the medium-chain dehydrogenase/reductases (MDRs) superfamily; AST2 has a paralog, AST1, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.95 0.98 0.97 0.93 0.89 0.87 0.75 0.74 0.78 0.69 0.65 0.66 0.93 0.82 0.81 0.94 0.9 0.96 0.82 0.94 0.79 0.77 0.81 0.82
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.06 0 0 0 0 0 0.05 0.06 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.08 0 0 0.06 0.12 0.3 0.39 0.34 0.3 0.45 0.49 0.47 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0.05 0.06 0.05 0.08 0.1 0.1 0.08 0.21 0.2 0.06 0.06 0 0.06 0 0 0.11 0.07 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 1 0 1 0 1 1 0 0 0 1 0 2 0 0 1 0 0 1 7 2 2 4
Bud 3 3 8 7 10 11 40 16 22 27 39 33 0 2 1 0 1 0 0 1 4 2 2 4
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 5 2 2 5
Bud Site 0 0 0 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 5 1 3 3 7 6 3 2 8 11 1 1 0 2 1 0 0 0 2 0 0 0
Cytoplasm 80 301 622 351 510 624 687 675 467 555 445 518 437 428 339 238 146 130 112 297 509 300 316 362
Endoplasmic Reticulum 0 2 1 1 0 1 0 3 2 8 2 2 5 7 9 8 6 1 3 0 17 0 2 2
Endosome 0 0 12 17 22 6 13 30 9 2 2 6 6 30 26 1 6 0 2 4 33 21 15 18
Golgi 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0 1 1 0 1 2 1 1 0 2
Mitochondria 7 3 7 23 70 214 358 310 179 362 337 372 0 0 0 3 6 0 1 1 23 5 3 6
Nucleus 0 0 1 0 2 0 2 3 5 1 0 3 2 1 1 3 2 2 0 0 0 1 2 2
Nuclear Periphery 1 0 1 0 1 1 0 6 0 1 1 0 0 0 0 0 0 0 1 0 2 1 0 0
Nucleolus 0 1 0 0 0 0 2 0 2 2 1 0 0 0 1 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 4 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 1 0 2 0 1 2 2 3 2 0 2 0 4 1 0 0 0 1 1 3 1 3 3
Vac/Vac Membrane 3 0 10 3 22 24 46 51 31 62 68 80 38 110 82 16 10 5 7 3 25 41 27 20
Unique Cell Count 84 308 643 379 575 719 913 912 597 807 689 787 468 525 418 253 163 135 136 317 646 389 389 440
Labelled Cell Count 94 312 668 406 645 885 1158 1103 723 1025 903 1030 489 585 460 272 180 138 136 317 646 389 389 440


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.5 5.6 5.2 5.0 4.9 4.3 4.2 4.3 4.3 4.2 4.1 4.1 6.6 6.9 6.6 7.6 7.6 7.2 6.5 6.7 7.4
Std Deviation (1e-4) 0.7 0.9 0.8 1.6 1.4 0.9 1.0 1.1 1.1 1.1 1.0 1.0 1.0 1.0 0.9 1.9 2.2 1.4 1.3 1.2 1.4
Intensity Change (Log2) – – – -0.06 -0.07 -0.28 -0.3 -0.27 -0.27 -0.3 -0.33 -0.32 0.34 0.4 0.35 0.55 0.56 0.47 0.32 0.36 0.51


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0.8 0.7 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -3.0 -5.5 -6.6 -11.4 -11.9 -10.0 -13.5 -14.7 -14.5 -2.6 -8.6 -8.5 -1.8 -3.9 -0.3
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 2.4 2.1 0 0 0 0 0 0 0 -0.8 3.5 3.7 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 4.6 7.9 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 2.5 2.1 3.6 4.0 3.6 5.3 6.5 6.7 5.3 10.9 10.2 3.8 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.511 4.3329 3.6029 3.6975 3.1042 3.8516 1.2529 1.4495 1.4434 0.9937 1.4494 1.1147 0.8699 1.0129 0.8023 0.9659 0.7622 0.5887
Actin 0.009 0.0007 0.0287 0.0224 0.0315 0.0016 0.0447 0.0026 0.0155 0.0065 0.0676 0.0013 0.0314 0.0066 0.0281 0.0135 0.0114 0.0046
Bud 0.0009 0.0005 0.004 0.0015 0.0016 0.0003 0.0015 0.0007 0.0003 0.0004 0.0005 0.0003 0.0005 0.0057 0.0016 0.0121 0.0037 0.0003
Bud Neck 0.0122 0.0002 0.0048 0.0006 0.0034 0.0002 0.0019 0.0002 0.0007 0.0015 0.0002 0.0008 0.0004 0.0002 0.0023 0.001 0.0003 0.0009
Bud Periphery 0.003 0.0004 0.0063 0.0026 0.0015 0.001 0.0023 0.0007 0.0003 0.0004 0.0006 0.0002 0.0003 0.0058 0.0016 0.0101 0.0039 0.0003
Bud Site 0.0059 0.0098 0.0071 0.0096 0.0103 0.0001 0.0065 0.0037 0.0054 0.0024 0.0007 0.0002 0.0015 0.0064 0.0112 0.0042 0.0056 0.0002
Cell Periphery 0.0004 0.0006 0.0011 0.0007 0.0002 0.0001 0.0003 0.0002 0.0002 0.0002 0 0.0001 0.0002 0.0003 0.0002 0.0002 0.0001 0.0001
Cytoplasm 0.4417 0.5276 0.4739 0.4974 0.4861 0.6663 0.3307 0.7334 0.6567 0.5432 0.8295 0.7852 0.3883 0.6941 0.6599 0.5108 0.6889 0.6829
Cytoplasmic Foci 0.0324 0.0187 0.031 0.0206 0.0599 0.0127 0.0952 0.0128 0.0204 0.1213 0.0097 0.0272 0.0271 0.011 0.0164 0.036 0.0418 0.0193
Eisosomes 0.0001 0.0001 0.0007 0.0016 0.0001 0 0.0004 0 0.0001 0.0001 0.0001 0 0.0002 0.0001 0.0001 0.0001 0 0.0001
Endoplasmic Reticulum 0.0037 0.0047 0.0049 0.0058 0.0026 0.0038 0.0095 0.0045 0.0048 0.0042 0.0068 0.011 0.0043 0.0056 0.0029 0.0025 0.0078 0.003
Endosome 0.0085 0.0193 0.0219 0.0254 0.0349 0.0096 0.0929 0.0056 0.016 0.0403 0.0068 0.0101 0.0222 0.013 0.0116 0.0516 0.0527 0.0161
Golgi 0.0017 0.0024 0.0042 0.0038 0.0105 0.0006 0.0222 0.0038 0.004 0.0251 0.0051 0.0019 0.0153 0.0004 0.0055 0.0165 0.0044 0.0015
Lipid Particles 0.0044 0.0019 0.0024 0.0049 0.0275 0.0011 0.0606 0.0008 0.0086 0.0256 0.0008 0.0025 0.02 0.0004 0.0054 0.0163 0.0011 0.0008
Mitochondria 0.0017 0.0017 0.0034 0.0348 0.0027 0.001 0.0155 0.0004 0.0056 0.0192 0.0139 0.0003 0.0071 0.001 0.0144 0.0076 0.004 0.001
None 0.4508 0.4042 0.3829 0.3221 0.3013 0.2968 0.1837 0.2232 0.2288 0.1394 0.0352 0.1461 0.4307 0.2424 0.2257 0.2823 0.1449 0.2605
Nuclear Periphery 0.0007 0.0009 0.0017 0.0061 0.0009 0.0006 0.0258 0.001 0.0013 0.001 0.0016 0.0013 0.019 0.0011 0.0009 0.0017 0.0041 0.0014
Nucleolus 0.0005 0.0002 0.0007 0.0015 0.0008 0.0001 0.0021 0.0002 0.0004 0.0006 0 0.0002 0.0021 0.0001 0.0002 0.0004 0.0002 0.0002
Nucleus 0.0016 0.0009 0.0024 0.0046 0.0013 0.0007 0.0042 0.0013 0.0011 0.001 0.0019 0.0016 0.0075 0.0015 0.0013 0.0027 0.0043 0.0019
Peroxisomes 0.0138 0.0005 0.0078 0.0154 0.0143 0.0013 0.0379 0.0014 0.0244 0.0563 0.0164 0.0046 0.0113 0.0001 0.0066 0.0228 0.0056 0.0003
Punctate Nuclear 0.0022 0.0004 0.0045 0.0061 0.0032 0.0005 0.0409 0.0008 0.0023 0.0037 0.0004 0.003 0.0055 0.0007 0.0009 0.0032 0.0028 0.0022
Vacuole 0.0044 0.0038 0.0049 0.0105 0.0045 0.0014 0.0108 0.0026 0.0025 0.006 0.0018 0.0019 0.0035 0.0031 0.0028 0.0036 0.0109 0.0021
Vacuole Periphery 0.0004 0.0007 0.0008 0.0022 0.0007 0.0002 0.0104 0.0002 0.0007 0.0018 0.0003 0.0003 0.0015 0.0004 0.0005 0.0009 0.0015 0.0005

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 4.6935 7.6286 7.925 7.8201 10.5111 6.8232 7.3276 8.3295 7.8806 10.4803
Translational Efficiency 2.1225 1.2159 1.1474 1.1359 0.8026 1.5716 1.8187 1.2887 1.6849 1.2691

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Lipid raft associated protein; overexpression restores Pma1p localization to lipid rafts which is required for targeting of Pma1p to the plasma membrane; sometimes classified in the medium-chain dehydrogenase/reductases (MDRs) superfamily; AST2 has a paralog, AST1, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (61%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Ast2

Ast2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ast2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available