Standard name
Human Ortholog
Description Heat shock protein that is highly induced upon stress; plays a role in SRP-dependent cotranslational protein-membrane targeting and translocation; member of the HSP70 family; cytoplasmic protein that concentrates in nuclei upon starvation; SSA4 has a paralog, SSA3, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.15
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0.05 0.05 0 0 0 0 0 0.05 0 0 0 0 0 0
Cytoplasm 0.97 0.96 0.95 0.96 0.94 0.84 0.8 – 0.58 0.7 0.64 0.62 0.98 0.98 0.97 0.89 0.92 0.92 0.93 0.9 0.85 0.91 0.87 0.72
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.06 0 0.07 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.06 0.06 – 0.15 0.06 0.18 0.08 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0.05 0 0.06 0.1 0.11 – 0.21 0.21 0.2 0.25 0 0 0 0.1 0.11 0.1 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 0 0 1 0 1 3 – 3 3 0 6 1 0 0 0 0 0 0 1 0 0 1 2
Bud 0 0 1 1 1 1 2 – 8 5 3 7 0 0 0 0 0 0 4 10 6 0 24 45
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 2
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 4 3 2 3 5 5 – 3 4 10 14 0 1 0 14 10 17 0 0 0 0 0 0
Cytoplasm 201 249 158 157 218 195 217 – 73 136 121 172 167 340 383 362 324 333 195 273 146 121 313 218
Endoplasmic Reticulum 1 0 0 0 0 0 4 – 2 2 7 1 0 0 1 24 8 24 0 1 4 0 0 1
Endosome 0 1 0 0 3 0 1 – 0 2 0 1 3 1 2 5 4 6 1 0 1 1 0 7
Golgi 0 0 1 0 0 1 1 – 0 2 1 2 0 0 0 2 1 1 0 0 0 0 0 2
Mitochondria 2 2 0 2 2 15 17 – 19 11 34 22 0 1 0 14 4 1 1 4 4 3 1 3
Nucleus 0 6 0 0 0 2 3 – 5 0 1 4 0 0 1 4 2 3 0 3 1 0 0 2
Nuclear Periphery 0 1 1 0 0 0 2 – 2 2 5 4 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 1 0 0 1 – 1 0 0 0 0 0 0 1 0 1 0 0 0 0 0 1
Peroxisomes 0 0 0 0 0 1 0 – 0 0 0 1 0 0 2 6 1 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 1 3 – 0 1 1 1 0 1 0 2 0 0 0 3 0 0 4 8
Vac/Vac Membrane 2 1 9 3 13 24 31 – 26 41 37 69 2 9 14 40 38 37 1 2 2 0 3 3
Unique Cell Count 207 260 166 163 231 232 271 125 195 189 278 170 348 395 405 351 362 210 305 173 133 359 305
Labelled Cell Count 207 264 173 167 240 246 290 142 209 220 304 173 353 403 474 392 423 210 305 173 133 359 305


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.8 8.2 12.0 10.4 11.5 15.5 16.7 – 17.0 27.3 22.2 31.8 9.5 11.1 9.1 16.7 18.6 16.9 6.6 8.6 10.8
Std Deviation (1e-4) 9.8 5.3 13.6 17.3 12.2 25.2 19.4 – 14.1 30.2 23.0 33.2 12.0 18.7 5.6 28.6 28.6 25.8 2.0 10.8 10.8
Intensity Change (Log2) – – – -0.21 -0.06 0.37 0.48 – 0.51 1.19 0.89 1.41 -0.33 -0.11 -0.4 0.48 0.64 0.5 -0.86 -0.48 -0.14


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 – 0 0 1.7 1.7 0 0 0 0 0 1.6
Cytoplasm 0.5 -0.4 -3.5 -4.4 – -7.7 -6.2 -7.1 -7.8 1.6 1.5 1.0 -2.2 -1.2 -1.3
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 3.2 0 3.4
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 3.3 3.3 – 5.2 3.1 5.7 3.7 0 0 0 0 0 0
Nucleus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole -1.7 0.1 1.8 2.1 – 4.0 4.3 4.0 5.2 -2.2 -1.6 -1.0 1.7 2.0 1.8

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.5198 3.1987 2.215 0.5236 1.3833 1.3719 1.7528 2.8592 2.4317 1.4332 3.2197 1.9211 1.8437 2.109 1.3014 1.4925 0.8138 1.3517
Actin 0.067 0.0015 0.0137 0.0482 0.0351 0.0012 0.0269 0.0014 0.0075 0.0206 0.024 0.0034 0.0815 0.0014 0.0091 0.1102 0.0197 0.0227
Bud 0.0007 0.0032 0.0026 0.0012 0.0013 0.0004 0.0011 0.0007 0.0054 0.0161 0.0062 0.0011 0.0031 0.0047 0.006 0.0019 0.0006 0.0016
Bud Neck 0.0037 0.0002 0.0005 0.0061 0.0015 0.0008 0.0068 0.0001 0.0013 0.0012 0.0058 0.0029 0.0007 0.0002 0.0005 0.0014 0.0063 0.0033
Bud Periphery 0.0009 0.0018 0.0022 0.0009 0.0026 0.0001 0.002 0.0011 0.0083 0.016 0.0073 0.0015 0.0041 0.0026 0.0033 0.0019 0.0008 0.002
Bud Site 0.0069 0.0038 0.0033 0.0182 0.0131 0 0.0088 0.0027 0.0231 0.0078 0.0083 0.0008 0.007 0.0064 0.0027 0.0081 0.0038 0.0007
Cell Periphery 0.0003 0.0002 0.0002 0.0005 0.0008 0 0.0005 0.0002 0.001 0.0002 0.0004 0.0001 0.0005 0.0002 0.0003 0.0002 0.0001 0.0002
Cytoplasm 0.4444 0.6985 0.5503 0.6025 0.4129 0.7085 0.408 0.6913 0.4728 0.5317 0.4687 0.6638 0.4379 0.73 0.6111 0.5456 0.4855 0.6476
Cytoplasmic Foci 0.032 0.0033 0.0207 0.0342 0.0298 0.0171 0.0325 0.0054 0.0235 0.02 0.0467 0.0105 0.0421 0.0082 0.0137 0.033 0.0316 0.0079
Eisosomes 0.0009 0.0001 0.0001 0.0002 0.0008 0 0.0004 0 0.0001 0.0001 0.0001 0 0.0006 0 0.0003 0.0002 0.0001 0.0001
Endoplasmic Reticulum 0.0092 0.0017 0.0025 0.0064 0.0058 0.0017 0.0061 0.0023 0.0029 0.0027 0.0061 0.0032 0.0091 0.0041 0.0042 0.0119 0.0026 0.0054
Endosome 0.0342 0.0005 0.0029 0.0191 0.0455 0.0029 0.0243 0.0027 0.0228 0.0241 0.0758 0.0059 0.044 0.0027 0.0055 0.0325 0.0272 0.0107
Golgi 0.0197 0.0001 0.003 0.014 0.0249 0.0003 0.0066 0.0002 0.0065 0.0047 0.0289 0.0012 0.0179 0.0004 0.0013 0.0195 0.0258 0.009
Lipid Particles 0.0189 0.0002 0.0039 0.0035 0.0954 0.0004 0.0155 0.0001 0.0025 0.0018 0.006 0.0038 0.0234 0.0009 0.0029 0.0076 0.0642 0.0036
Mitochondria 0.0219 0.0002 0.0025 0.0029 0.0765 0.0002 0.0203 0.0003 0.0068 0.0061 0.0102 0.0012 0.0138 0.0005 0.004 0.0498 0.0126 0.0048
None 0.2802 0.2802 0.3684 0.2282 0.1659 0.2554 0.3772 0.2851 0.3879 0.3077 0.2746 0.2876 0.2575 0.2271 0.3039 0.1435 0.2733 0.2569
Nuclear Periphery 0.0155 0.0006 0.001 0.0018 0.0265 0.0012 0.019 0.001 0.0023 0.0025 0.0035 0.0016 0.0096 0.0025 0.0058 0.0049 0.002 0.0038
Nucleolus 0.0012 0.0001 0.0003 0.0002 0.0011 0.0001 0.001 0.0001 0.0007 0.0007 0.0004 0.0011 0.0046 0.0003 0.0011 0.0003 0.0006 0.0002
Nucleus 0.0059 0.0021 0.002 0.002 0.0035 0.0024 0.0132 0.0026 0.0083 0.0078 0.0038 0.0049 0.005 0.0036 0.0049 0.0052 0.0012 0.0027
Peroxisomes 0.0245 0.0001 0.012 0.005 0.044 0.0005 0.0155 0.0002 0.0098 0.0138 0.0119 0.0014 0.0162 0.0003 0.0093 0.011 0.0312 0.0015
Punctate Nuclear 0.0069 0.0008 0.0069 0.0026 0.005 0.0064 0.0058 0.0007 0.0036 0.0037 0.0048 0.0012 0.0051 0.0026 0.008 0.0028 0.0094 0.0139
Vacuole 0.0033 0.0007 0.0007 0.0019 0.0039 0.0004 0.0045 0.0016 0.0024 0.0087 0.0045 0.0022 0.0082 0.0012 0.0017 0.006 0.0009 0.0009
Vacuole Periphery 0.0017 0.0001 0.0001 0.0004 0.0039 0.0001 0.004 0.0002 0.0007 0.0021 0.0018 0.0004 0.0081 0.0003 0.0007 0.0025 0.0006 0.0005

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 7.4361 9.2041 9.4859 9.1217 10.0121 9.7188 10.5988 12.7367 16.3935 8.6975
Translational Efficiency 1.2013 1.508 1.1749 1.6903 1.2399 0.902 1.1872 1.4673 1.2032 0.9056

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Heat shock protein that is highly induced upon stress; plays a role in SRP-dependent cotranslational protein-membrane targeting and translocation; member of the HSP70 family; cytoplasmic protein that concentrates in nuclei upon starvation; SSA4 has a paralog, SSA3, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (63%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Ssa4

Ssa4


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ssa4-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available