Standard name
Human Ortholog
Description Karyopherin beta; mediates nuclear import of ribosomal proteins prior to assembly into ribosomes and import of histones H3 and H4; localizes to the nuclear pore, nucleus, and cytoplasm; exhibits genetic interactions with RAI1
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0.09 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.89 0.87 0.87 0.96 0.91 0.94 0.88 0.8 0.89 – 0.81 0.81 0.97 0.99 0.97 0.97 0.95 0.96 0.61 0.64 0.74 0.82 0.82 0.84
Endoplasmic Reticulum 0 0 0 0 0.07 0.05 0.09 0.15 0.08 – 0.11 0.09 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.2 0.17 0.06 0.06 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.16 0.1 0.1 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.07 0.12 0.1 0.05 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 0 0 1 0 3 5 2 – 2 11 0 0 0 0 0 0 0 1 0 0 0 0
Bud 0 0 1 1 1 0 2 1 0 – 0 0 0 0 1 1 1 0 3 5 7 2 1 5
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 2 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 1 4 5 12 10 2 – 9 26 0 0 0 0 2 2 0 0 0 0 0 0
Cytoplasm 140 235 315 218 254 328 347 282 171 – 191 240 190 157 170 136 121 81 112 219 302 92 134 188
Endoplasmic Reticulum 1 11 13 8 20 16 37 52 16 – 26 28 4 2 3 4 3 3 6 4 13 0 7 9
Endosome 1 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 1 0 0 0 0
Golgi 2 0 0 1 1 0 0 2 0 – 0 2 1 0 1 0 1 0 0 0 1 0 0 2
Mitochondria 0 0 2 0 0 0 0 1 5 – 7 6 0 0 0 1 0 1 0 0 0 0 0 0
Nucleus 1 3 0 0 0 1 2 1 0 – 3 1 1 0 1 0 1 0 36 58 24 6 3 3
Nuclear Periphery 0 1 0 0 0 0 0 0 0 – 0 0 0 0 0 1 0 0 6 4 6 0 1 1
Nucleolus 0 0 0 0 0 0 0 1 0 – 2 0 0 0 0 0 0 0 1 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 – 0 0 1 0 0 0 0 0 0 0 2 0 0 0
Vac/Vac Membrane 26 26 37 4 9 1 3 9 2 – 7 4 1 1 1 2 5 3 13 40 40 5 6 7
Unique Cell Count 158 269 362 228 280 348 395 351 193 237 298 196 159 175 140 128 84 186 344 407 113 163 226
Labelled Cell Count 171 277 368 233 290 351 406 364 198 247 318 198 160 177 145 134 90 186 344 407 113 163 226


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 77.4 63.4 42.7 42.6 38.5 33.1 33.4 30.7 27.7 – 27.1 30.5 60.1 51.4 43.6 102.8 107.5 111.1 65.6 68.1 67.1
Std Deviation (1e-4) 17.6 15.6 10.8 9.0 9.5 8.2 8.6 7.2 8.6 – 7.6 7.4 13.3 12.2 11.7 37.4 34.3 28.5 18.5 21.4 20.7
Intensity Change (Log2) – – – -0.0 -0.15 -0.37 -0.35 -0.48 -0.62 – -0.65 -0.48 0.49 0.27 0.03 1.27 1.33 1.38 0.62 0.67 0.65


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 3.3 0 0 – 0 5.7 0 0 0 0 0 0
Cytoplasm 3.4 1.5 3.3 0.3 -2.4 0.5 – -2.1 -2.3 3.8 4.2 3.7 3.4 2.3 2.5
Endoplasmic Reticulum -0.1 2.0 0.7 3.2 5.2 2.4 – 3.6 3.1 -1.0 0 -1.2 0 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Vacuole -3.9 -3.4 -5.9 -5.8 -4.2 -4.0 – -3.3 -4.7 -4.3 -3.9 -4.1 -3.3 -2.2 -1.9

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration – – – – – – – – – – – – – – – – – –
Actin 0.0587 0.0001 0.0085 0.0003 0.0782 0.0001 0.0007 0.0002 0.0005 0.0001 0.0098 0.0016 0.0034 0.0275 0 0.0002 0.0003 0.0002
Bud 0.0013 0 0.0004 0.0001 0.0005 0 0.0006 0 0.0002 0.0002 0.0008 0 0.0012 0.0034 0 0.0001 0 0
Bud Neck 0.0045 0.0002 0.0006 0.0003 0.001 0.0005 0.0004 0.0002 0.0004 0.0002 0.0008 0.0005 0.0008 0.0005 0.0001 0.0002 0.0009 0.0002
Bud Periphery 0.0013 0 0.0005 0.0001 0.0004 0 0.0006 0 0.0002 0.0002 0.0006 0 0.0006 0.0077 0 0 0 0
Bud Site 0.002 0 0.0045 0.0006 0.0008 0 0.0006 0 0.0016 0.0004 0.003 0 0.0016 0.0065 0 0 0 0
Cell Periphery 0.0016 0 0.0005 0 0.0006 0 0.0005 0.001 0.0009 0.0016 0.0012 0 0.0011 0.0007 0 0 0 0
Cytoplasm 0.2429 0.6131 0.5897 0.6722 0.5097 0.4305 0.5605 0.7358 0.7205 0.736 0.7321 0.6474 0.5399 0.6198 0.7973 0.8471 0.6917 0.4051
Cytoplasmic Foci 0.0293 0 0.0055 0.0001 0.0065 0.0001 0.0021 0.0001 0.0017 0.0012 0.0178 0.0002 0.0864 0.0015 0.0001 0.0004 0 0.0001
Eisosomes 0.0014 0 0.0005 0 0.0003 0 0.0001 0 0 0.0001 0.0003 0 0.0002 0.001 0 0 0 0
Endoplasmic Reticulum 0.0081 0.0006 0.0019 0.0005 0.0023 0.0008 0.0044 0.0009 0.0018 0.0011 0.0046 0.0015 0.0323 0.0044 0.0001 0.0009 0.0004 0.0004
Endosome 0.0136 0.0001 0.0036 0.0002 0.0076 0.0002 0.0016 0.0001 0.0013 0.0015 0.0203 0.0021 0.059 0.0119 0 0.0006 0 0.0001
Golgi 0.0104 0 0.0013 0 0.0082 0 0.0001 0 0.0018 0.0009 0.0044 0.0004 0.0021 0.0027 0 0 0 0
Lipid Particles 0.0123 0 0.0036 0 0.0083 0 0.0007 0.0003 0.0072 0.0218 0.0075 0.0001 0.0081 0.0022 0 0 0 0
Mitochondria 0.0145 0.0002 0.0073 0.0003 0.0053 0.0001 0.0026 0.0001 0.0065 0.0027 0.0054 0.0008 0.0036 0.1789 0 0.0002 0.0001 0.0002
None 0.0294 0.0001 0.0049 0.0003 0.0031 0.0001 0.0024 0.0002 0.0007 0.0015 0.0094 0.0002 0.1561 0.0039 0.0001 0.0002 0.0001 0.0001
Nuclear Periphery 0.139 0.0793 0.0836 0.0634 0.0869 0.1374 0.1251 0.0449 0.0582 0.0497 0.0302 0.0693 0.0423 0.0406 0.0462 0.0567 0.0433 0.143
Nucleolus 0.0291 0.0002 0.0045 0.001 0.0215 0.0002 0.0045 0.0003 0.0028 0.0101 0.0052 0.0001 0.0032 0.006 0 0.0001 0 0.0002
Nucleus 0.3657 0.3029 0.2559 0.2573 0.2227 0.4269 0.2788 0.2139 0.1853 0.1614 0.1068 0.2733 0.0189 0.0624 0.1557 0.0897 0.2622 0.448
Peroxisomes 0.002 0 0.0009 0 0.0056 0 0.0001 0 0.0008 0.0018 0.0014 0 0.0013 0.0016 0 0 0 0
Punctate Nuclear 0.0139 0.0002 0.0015 0.0014 0.0045 0.0003 0.0015 0.0003 0.0008 0.0005 0.0108 0.001 0.0104 0.0025 0.0002 0.0007 0.0006 0.0012
Vacuole 0.0119 0.0021 0.0092 0.0011 0.0132 0.0014 0.0078 0.0016 0.0038 0.005 0.0173 0.0007 0.0228 0.0057 0.0001 0.0012 0.0001 0.0004
Vacuole Periphery 0.0072 0.0009 0.0107 0.0008 0.0128 0.0014 0.0043 0.0002 0.0028 0.002 0.0103 0.0006 0.0047 0.0086 0 0.0016 0.0002 0.0008

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 342.7091 352.1085 368.1569 356.9107 285.7372 238.9056 310.7312 264.9605 236.2419 254.8009
Translational Efficiency 1.1558 1.3382 1.2768 1.1469 1.2454 1.3262 1.167 1.083 1.1693 1.0741

Hit Data

Dataset Hit
Protein Concentration –
Protein Localization ✔
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
359 1084 682 1220 1697 1567 515 160 2056 2651 1197 1380

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 3280.15 4004.11 4715.15 6393.91 3412.57 4018.89 5750.27 6839.72 3389.45 4012.85 5160.50 6445.60
Standard Deviation 718.40 984.62 1117.44 1708.45 684.79 916.80 1065.76 1516.91 692.60 945.15 1209.45 1693.38
Intensity Change Log 2 — 0.287720 0.523542 0.962937 — 0.235938 0.752771 1.003079 — 0.261549 0.644966 0.983544

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000411 0.000168 0.000293 0.001361 0.000110 0.000150 0.000225 0.000319 0.000162 0.000157 0.000264 0.001240
Bud Neck 0.009296 0.003880 0.010560 0.013681 0.003640 0.005050 0.010980 0.005905 0.004627 0.004571 0.010741 0.012779
Bud Site 0.000837 0.000488 0.000392 0.007475 0.000368 0.000592 0.000598 0.001566 0.000450 0.000549 0.000481 0.006790
Cell Periphery 0.000722 0.000335 0.000510 0.000755 0.000217 0.000248 0.000686 0.000316 0.000305 0.000283 0.000586 0.000705
Cytoplasm 0.280258* 0.385930* 0.116532* 0.059005 0.528023* 0.379992* 0.150597* 0.016892 0.484760* 0.382420* 0.131188* 0.054123
Cytoplasmic Foci 0.001579 0.001724 0.001608 0.016160 0.001207 0.001989 0.001480 0.004704 0.001272 0.001881 0.001553 0.014831
Eisosomes 0.000008 0.000002 0.000005 0.000017 0.000001 0.000002 0.000005 0.000024 0.000002 0.000002 0.000005 0.000017
Endoplasmic Reticulum 0.001585 0.000567 0.000334 0.000314 0.002623 0.000639 0.000680 0.000185 0.002441 0.000610 0.000483 0.000299
Endosome 0.000651 0.000354 0.000199 0.003283 0.000590 0.000604 0.000498 0.001135 0.000601 0.000502 0.000328 0.003034
Golgi 0.000478 0.000466 0.000296 0.005093 0.000383 0.001148 0.000317 0.000511 0.000400 0.000869 0.000305 0.004562
Lipid Particles 0.001299 0.000582 0.000386 0.001305 0.000525 0.000602 0.000853 0.000106 0.000660 0.000594 0.000587 0.001166
Mitochondria 0.001805 0.001173 0.002563 0.003634 0.001232 0.001274 0.002476 0.001335 0.001332 0.001232 0.002526 0.003367
Mitotic Spindle 0.000862 0.006046 0.001789 0.024381 0.002594 0.007103 0.005222 0.031449 0.002292 0.006671 0.003266 0.025201
None 0.008874 0.007673 0.003363 0.012195 0.005219 0.006960 0.005097 0.004644 0.005857 0.007252 0.004109 0.011319
Nuclear Periphery 0.009197 0.017112 0.018486 0.001629 0.033926 0.018618 0.057709 0.000573 0.029608 0.018002 0.035361 0.001506
Nuclear Periphery Foci 0.014467 0.016790 0.005632 0.000860 0.022576 0.014727 0.017052 0.000145 0.021160 0.015571 0.010546 0.000777
Nucleolus 0.001493 0.000736 0.000813 0.003279 0.000667 0.000903 0.000544 0.000957 0.000811 0.000835 0.000697 0.003010
Nucleus 0.657928* 0.550937* 0.832172* 0.836276* 0.390570* 0.552777* 0.737600* 0.927626* 0.437254* 0.552025* 0.791483* 0.846867*
Peroxisomes 0.000034 0.000017 0.000028 0.000319 0.000011 0.000178 0.000023 0.000089 0.000015 0.000112 0.000026 0.000292
Vacuole 0.007562 0.004700 0.003724 0.006037 0.004947 0.005354 0.007123 0.001423 0.005403 0.005087 0.005186 0.005502
Vacuole Periphery 0.000655 0.000320 0.000313 0.002942 0.000572 0.001092 0.000236 0.000097 0.000587 0.000777 0.000280 0.002612

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 4.28 0.67 -0.43 -2.11 -0.71 -2.41 -9.75 -8.04 -6.72 -0.13 0.24 -9.80 -2.38 -2.42 -0.77
Bud Neck 3.74 -2.37 -2.93 -9.83 -0.47 -2.03 -9.76 -4.19 -3.77 1.11 0.00 -11.36 -10.16 -10.77 1.08
Bud Site 2.57 2.29 -5.61 -7.00 -6.90 -2.27 -6.55 -4.82 -4.51 -2.89 -0.68 -5.18 -7.77 -7.66 -6.56
Cell Periphery 2.64 -0.20 1.65 -2.43 3.13 -1.57 -9.70 -3.40 -2.91 4.32 -0.02 -9.62 -4.12 -4.59 6.34
Cytoplasm -6.22 12.15 15.93 37.38 7.48 16.46 47.14 68.42* 46.58 14.15 13.49 50.57 63.27 55.87 11.80
Cytoplasmic Foci -0.19 -1.24 -4.24 -4.10 -3.57 -2.56 -6.06 -2.78 -2.33 -1.32 -2.82 -6.13 -5.56 -4.56 -3.15
Eisosomes 3.58 -0.33 -3.49 -13.75 -4.97 -2.58 -7.79 -8.24 -7.99 -4.95 0.54 -9.62 -15.26 -15.78 -5.09
Endoplasmic Reticulum 4.21 5.18 5.69 4.33 3.45 7.85 7.86 9.33 5.93 5.29 8.58 9.24 10.29 7.45 6.26
Endosome 2.15 2.79 0.09 -1.40 -1.83 -0.50 -1.13 1.83 1.95 2.22 1.20 2.78 -0.34 -0.70 -1.06
Golgi 0.16 0.99 -1.04 -1.07 -1.19 -2.58 -1.91 0.89 2.81 2.09 -2.59 -0.89 -1.16 -0.44 -1.07
Lipid Particles 1.36 1.38 1.50 0.46 0.36 -1.13 -4.95 0.04 0.63 3.83 -0.02 -2.83 0.48 0.64 3.49
Mitochondria 2.45 -4.65 -1.83 -4.71 2.46 -0.88 -12.36 -4.17 -3.66 4.46 0.89 -13.69 -4.75 -5.06 2.71
Mitotic Spindle -2.84 -1.06 -6.01 -3.62 -5.37 -3.58 -2.86 -1.97 -1.15 -1.12 -4.29 -2.51 -6.19 -3.69 -4.64
None 0.53 2.72 -1.31 -4.22 -11.35 -3.69 -0.89 -1.87 -0.81 -1.60 -2.66 3.10 -6.75 -5.30 -10.06
Nuclear Periphery -3.20 -4.45 3.20 8.00 7.83 5.10 -2.31 13.44 9.63 9.17 4.55 -1.41 13.42 12.14 11.82
Nuclear Periphery Foci -0.36 2.49 4.55 8.46 5.05 2.80 2.90 11.07 9.66 7.21 2.07 5.21 12.03 12.87 8.66
Nucleolus 1.54 1.13 -1.40 -4.05 -3.58 -0.86 -0.99 -1.13 -1.01 -1.01 -0.06 -0.60 -3.72 -3.91 -3.74
Nucleus 5.41 -9.98 -12.06 -27.30 -2.81 -16.30 -26.49 -28.44 -18.67 -7.87 -13.51 -37.11 -46.99 -36.17 -7.80
Peroxisomes 1.29 -0.51 -3.64 -4.87 -3.82 -1.11 -7.42 -3.83 0.67 -2.13 -1.11 -7.83 -5.70 0.32 -3.94
Vacuole 1.63 0.80 -2.55 -4.63 -3.77 -2.40 -7.40 -2.13 -1.58 2.58 -1.51 -6.95 -5.84 -5.04 0.38
Vacuole Periphery 1.54 1.12 -0.21 -1.13 -0.89 -1.74 3.43 6.63 4.05 4.94 -0.89 2.96 -0.14 0.29 -0.93
Standard name
Human Ortholog
Description Karyopherin beta; mediates nuclear import of ribosomal proteins prior to assembly into ribosomes and import of histones H3 and H4; localizes to the nuclear pore, nucleus, and cytoplasm; exhibits genetic interactions with RAI1
Localization
Cell Percentages cytoplasm (40%), ER (3%), mixed (55%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Kap123

Kap123


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Kap123-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available