Standard name | |
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Human Ortholog | |
Description | Protein implicated in polar growth, functionally redundant with Boi1p; interacts with bud-emergence protein Bem1p; contains an SH3 (src homology 3) domain and a PH (pleckstrin homology) domain; BOI2 has a paralog, BOI1, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 133 | 78 | 26 | 18 | 50 | 35 | 25 | 42 | 29 | 42 | 20 | 23 | 146 | 216 | 216 | 118 | 52 | 125 | 129 | 54 | 29 | 44 | 80 | 156 |
Bud | 37 | 28 | 20 | 16 | 42 | 21 | 30 | 48 | 17 | 27 | 27 | 15 | 52 | 95 | 98 | 60 | 30 | 50 | 73 | 42 | 21 | 83 | 123 | 182 |
Bud Neck | 2 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 49 | 37 | 16 | 58 | 120 | 131 |
Bud Site | 41 | 17 | 1 | 1 | 17 | 8 | 9 | 8 | 10 | 10 | 3 | 12 | 11 | 11 | 6 | 4 | 2 | 5 | – | – | – | – | – | – |
Cell Periphery | 27 | 14 | 3 | 4 | 9 | 10 | 16 | 25 | 28 | 36 | 19 | 37 | 46 | 90 | 169 | 45 | 36 | 52 | 17 | 6 | 2 | 1 | 2 | 8 |
Cytoplasm | 62 | 45 | 17 | 43 | 97 | 90 | 178 | 211 | 93 | 111 | 68 | 94 | 74 | 173 | 216 | 100 | 61 | 139 | 18 | 15 | 16 | 20 | 32 | 26 |
Endoplasmic Reticulum | 10 | 19 | 3 | 6 | 14 | 15 | 20 | 19 | 14 | 22 | 7 | 19 | 18 | 48 | 35 | 16 | 11 | 10 | 9 | 2 | 0 | 1 | 12 | 11 |
Endosome | 4 | 2 | 0 | 0 | 1 | 1 | 8 | 4 | 0 | 0 | 0 | 1 | 2 | 0 | 1 | 7 | 3 | 16 | 6 | 1 | 2 | 3 | 10 | 5 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 8 | 3 | 12 | 2 | 0 | 0 | 0 | 3 | 0 |
Mitochondria | 6 | 7 | 17 | 10 | 20 | 41 | 80 | 113 | 118 | 173 | 74 | 101 | 8 | 13 | 28 | 14 | 8 | 13 | 4 | 2 | 3 | 0 | 5 | 5 |
Nucleus | 2 | 0 | 0 | 0 | 0 | 2 | 1 | 3 | 3 | 3 | 2 | 0 | 1 | 0 | 1 | 2 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 3 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 3 | 6 |
Nucleolus | 1 | 0 | 0 | 0 | 0 | 1 | 4 | 2 | 4 | 1 | 1 | 1 | 0 | 1 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 24 | 15 | 0 | 0 | 9 | 1 | 7 | 2 | 3 | 4 | 2 | 3 | 22 | 8 | 3 | 11 | 2 | 7 | 0 | 0 | 0 | 0 | 0 | 1 |
SpindlePole | 14 | 1 | 0 | 1 | 3 | 3 | 5 | 4 | 0 | 1 | 0 | 13 | 9 | 7 | 2 | 10 | 8 | 28 | 0 | 0 | 3 | 1 | 2 | 2 |
Vac/Vac Membrane | 4 | 0 | 0 | 0 | 3 | 2 | 9 | 10 | 4 | 8 | 2 | 21 | 2 | 1 | 3 | 10 | 5 | 33 | 1 | 3 | 0 | 6 | 2 | 3 |
Unique Cell Count | 250 | 151 | 61 | 69 | 189 | 169 | 303 | 365 | 231 | 315 | 161 | 243 | 264 | 448 | 523 | 290 | 152 | 361 | 315 | 173 | 98 | 223 | 404 | 545 |
Labelled Cell Count | 367 | 226 | 87 | 99 | 266 | 231 | 392 | 492 | 323 | 440 | 227 | 343 | 391 | 663 | 779 | 407 | 226 | 492 | 315 | 173 | 98 | 223 | 404 | 545 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 9.8 | 7.7 | 5.5 | 5.8 | 5.6 | 5.1 | 5.0 | 4.9 | 4.6 | 4.7 | 5.0 | 7.6 | 8.4 | 7.9 | 7.0 | 11.0 | 11.4 | 12.7 | 8.4 | 8.3 | 8.5 |
Std Deviation (1e-4) | 1.6 | 1.5 | 0.7 | 0.7 | 1.1 | 0.8 | 1.1 | 0.9 | 0.8 | 0.9 | 1.3 | 6.2 | 1.6 | 1.6 | 1.5 | 2.7 | 2.8 | 3.3 | 1.2 | 1.5 | 1.3 |
Intensity Change (Log2) | – | – | – | 0.09 | 0.02 | -0.1 | -0.13 | -0.16 | -0.24 | -0.22 | -0.15 | 0.48 | 0.62 | 0.52 | 0.36 | 1.0 | 1.05 | 1.21 | 0.62 | 0.59 | 0.63 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | -2.0 | -2.4 | -3.3 | -7.1 | -6.1 | 0 | 0 | -5.0 | -6.3 | 1.8 | 0.8 | -0.2 | -0.3 | -1.2 | -1.2 |
Bud | -1.2 | -1.7 | -3.6 | -4.7 | -3.9 | 0 | 0 | -2.6 | -5.8 | -2.2 | -2.0 | -2.6 | -2.0 | -2.0 | -3.7 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.5 | 2.1 | 2.5 | 2.9 | 4.4 | 2.2 | 3.2 | 2.0 |
Cytoplasm | 3.9 | 3.2 | 3.4 | 4.4 | 4.3 | 1.8 | 1.1 | 2.0 | 1.6 | 0 | 1.6 | 2.0 | 1.0 | 1.7 | 1.6 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.4 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | -1.9 | -3.3 | -0.6 | -0.2 | 0.5 | 0 | 0 | 2.4 | 2.0 | 0 | 0 | 0 | -5.8 | -4.6 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 3.5801 | 4.8652 | 4.0844 | 4.4785 | 3.1284 | 4.2669 | 4.5929 | 5.7732 | 5.6329 | 5.3741 | 5.6624 | 5.5062 | 2.4985 | 3.7343 | 3.4824 | 2.8827 | 2.5785 | 3.0987 |
Actin | 0.2684 | 0.1103 | 0.2355 | 0.2733 | 0.3043 | 0.2195 | 0.21 | 0.0838 | 0.2019 | 0.2507 | 0.2979 | 0.158 | 0.1749 | 0.1648 | 0.1978 | 0.3976 | 0.2085 | 0.1928 |
Bud | 0.0088 | 0.0378 | 0.1723 | 0.088 | 0.0155 | 0.009 | 0.0247 | 0.0155 | 0.2275 | 0.0888 | 0.0528 | 0.0268 | 0.0093 | 0.0234 | 0.1777 | 0.0586 | 0.0828 | 0.005 |
Bud Neck | 0.0725 | 0.0064 | 0.0159 | 0.0042 | 0.0671 | 0.5149 | 0.1028 | 0.0116 | 0.0137 | 0.0071 | 0.0037 | 0.5784 | 0.0788 | 0.0089 | 0.0105 | 0.0012 | 0.1078 | 0.4478 |
Bud Periphery | 0.0189 | 0.0527 | 0.3639 | 0.4732 | 0.1102 | 0.0379 | 0.0505 | 0.0156 | 0.3909 | 0.3331 | 0.4001 | 0.0897 | 0.0706 | 0.0365 | 0.3906 | 0.4575 | 0.3696 | 0.0152 |
Bud Site | 0.1568 | 0.3234 | 0.0801 | 0.0176 | 0.0286 | 0.0124 | 0.1886 | 0.4556 | 0.0595 | 0.0175 | 0.0115 | 0.0254 | 0.1223 | 0.3263 | 0.0987 | 0.054 | 0.0336 | 0.0127 |
Cell Periphery | 0.0184 | 0.025 | 0.009 | 0.0139 | 0.0044 | 0.0079 | 0.009 | 0.0121 | 0.0038 | 0.0066 | 0.0411 | 0.0095 | 0.0857 | 0.0116 | 0.0067 | 0.0056 | 0.0286 | 0.0048 |
Cytoplasm | 0.2456 | 0.4172 | 0.0581 | 0.0376 | 0.0074 | 0.1679 | 0.2163 | 0.376 | 0.0673 | 0.0219 | 0.027 | 0.0603 | 0.3524 | 0.3713 | 0.088 | 0.0164 | 0.1315 | 0.2653 |
Cytoplasmic Foci | 0.0385 | 0.0086 | 0.0094 | 0.0065 | 0.0162 | 0.008 | 0.04 | 0.0097 | 0.0057 | 0.03 | 0.0564 | 0.0168 | 0.0157 | 0.0038 | 0.002 | 0.0036 | 0.0259 | 0.0156 |
Eisosomes | 0.002 | 0.0009 | 0.0004 | 0.0009 | 0.003 | 0.0006 | 0.002 | 0.0003 | 0.0005 | 0.0005 | 0.0004 | 0.0004 | 0.0064 | 0.0007 | 0.0007 | 0.0012 | 0.0007 | 0.0008 |
Endoplasmic Reticulum | 0.021 | 0.002 | 0.0011 | 0.0012 | 0.008 | 0.0009 | 0.0095 | 0.0017 | 0.0004 | 0.0021 | 0.0005 | 0.0017 | 0.0075 | 0.0026 | 0.0017 | 0.0002 | 0.0014 | 0.0018 |
Endosome | 0.0379 | 0.002 | 0.0073 | 0.0143 | 0.0633 | 0.002 | 0.0395 | 0.0015 | 0.0023 | 0.0686 | 0.0394 | 0.0059 | 0.015 | 0.0017 | 0.0009 | 0.0001 | 0.0016 | 0.0011 |
Golgi | 0.0221 | 0.0038 | 0.0069 | 0.0214 | 0.0253 | 0.0039 | 0.0194 | 0.0024 | 0.003 | 0.0399 | 0.0416 | 0.0143 | 0.0075 | 0.0026 | 0.0007 | 0.0007 | 0.0027 | 0.0035 |
Lipid Particles | 0.0106 | 0.0006 | 0.0056 | 0.0094 | 0.0316 | 0.0027 | 0.0085 | 0.0006 | 0.0013 | 0.0305 | 0.0042 | 0.0007 | 0.0084 | 0.0005 | 0.0001 | 0 | 0.0002 | 0.0025 |
Mitochondria | 0.0082 | 0.0006 | 0.0174 | 0.0062 | 0.0674 | 0.005 | 0.0132 | 0.0004 | 0.0142 | 0.024 | 0.0053 | 0.0029 | 0.0058 | 0.0004 | 0.0006 | 0.0005 | 0.0009 | 0.0007 |
None | 0.0097 | 0.0047 | 0.0016 | 0.0263 | 0.0191 | 0.0016 | 0.0287 | 0.0085 | 0.0009 | 0.001 | 0.0004 | 0.0006 | 0.0082 | 0.0418 | 0.0213 | 0.0008 | 0.0019 | 0.0172 |
Nuclear Periphery | 0.0282 | 0.0004 | 0.0006 | 0.0011 | 0.0556 | 0.0007 | 0.0116 | 0.0003 | 0.0005 | 0.0078 | 0.0002 | 0.0002 | 0.0014 | 0.0004 | 0.0002 | 0.0001 | 0.0003 | 0.0001 |
Nucleolus | 0.0008 | 0.0001 | 0.0016 | 0.0001 | 0.0164 | 0.0001 | 0.0008 | 0.0001 | 0.0002 | 0.0041 | 0.0001 | 0.0001 | 0.0004 | 0.0001 | 0 | 0 | 0.0001 | 0.0001 |
Nucleus | 0.007 | 0.0007 | 0.0009 | 0.0004 | 0.0254 | 0.0004 | 0.0029 | 0.0008 | 0.0025 | 0.0297 | 0.0001 | 0.0006 | 0.0012 | 0.0007 | 0.0003 | 0.0001 | 0.0004 | 0.0003 |
Peroxisomes | 0.0094 | 0.001 | 0.0065 | 0.002 | 0.074 | 0.003 | 0.0062 | 0.0009 | 0.0026 | 0.0199 | 0.0152 | 0.0062 | 0.0152 | 0.0002 | 0.0006 | 0.0007 | 0.0004 | 0.0118 |
Punctate Nuclear | 0.0097 | 0.0003 | 0.0037 | 0.0007 | 0.044 | 0.0006 | 0.0117 | 0.0006 | 0.0002 | 0.0023 | 0.0006 | 0.0003 | 0.0043 | 0.0006 | 0.0001 | 0.0001 | 0.0004 | 0.0003 |
Vacuole | 0.0031 | 0.0012 | 0.0018 | 0.0011 | 0.0066 | 0.0008 | 0.0027 | 0.0019 | 0.001 | 0.009 | 0.0011 | 0.0009 | 0.0085 | 0.001 | 0.0006 | 0.0007 | 0.0007 | 0.0004 |
Vacuole Periphery | 0.0024 | 0.0001 | 0.0004 | 0.0006 | 0.0066 | 0.0002 | 0.0013 | 0.0001 | 0.0002 | 0.0049 | 0.0004 | 0.0002 | 0.0006 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 27.5586 | 38.2169 | 27.1868 | 24.2987 | 26.1111 | 25.0715 | 36.8049 | 32.6541 | 25.6521 | 29.2655 |
Translational Efficiency | 1.2231 | 1.1725 | 1.2404 | 1.0896 | 1.2927 | 1.1935 | 0.9993 | 0.9607 | 1.0136 | 0.9597 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✔ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1320 | 1083 | 161 | 901 | 1545 | 1045 | 1562 | 1317 | 2865 | 2128 | 1723 | 2218 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 807.15 | 976.63 | 1336.70 | 1047.51 | 802.19 | 892.93 | 1110.01 | 1102.45 | 804.48 | 935.53 | 1131.19 | 1080.13 |
Standard Deviation | 108.56 | 142.28 | 130.92 | 160.25 | 133.20 | 123.69 | 148.50 | 161.60 | 122.49 | 139.88 | 161.08 | 163.30 |
Intensity Change Log 2 | — | 0.274975 | 0.727767 | 0.376055 | — | 0.154603 | 0.468557 | 0.458697 | — | 0.216230 | 0.604374 | 0.417841 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.061145 | 0.072086 | 0.246639* | 0.091528 | 0.165664* | 0.060209 | 0.060212 | 0.077602 | 0.117509* | 0.066254 | 0.077632 | 0.083259 |
Bud Neck | 0.127609 | 0.070058 | 0.097939 | 0.022449 | 0.088552 | 0.125146 | 0.048665 | 0.028821 | 0.106547 | 0.097110 | 0.053269 | 0.026232 |
Bud Site | 0.539466* | 0.616821* | 0.542024* | 0.645120* | 0.439782* | 0.556676* | 0.729001* | 0.702284* | 0.485709* | 0.587285* | 0.711529* | 0.679063* |
Cell Periphery | 0.050859 | 0.008668 | 0.031329 | 0.017303 | 0.040953 | 0.016980 | 0.009823 | 0.030583 | 0.045517 | 0.012750 | 0.011833 | 0.025189 |
Cytoplasm | 0.128101* | 0.151528* | 0.022161 | 0.134980* | 0.147187* | 0.140500* | 0.110939* | 0.122901* | 0.138393* | 0.146112* | 0.102643* | 0.127808* |
Cytoplasmic Foci | 0.038312 | 0.032262 | 0.008238 | 0.013457 | 0.041509 | 0.032362 | 0.008238 | 0.003909 | 0.040036 | 0.032311 | 0.008238 | 0.007788 |
Eisosomes | 0.007567 | 0.001017 | 0.011128 | 0.001827 | 0.009026 | 0.001917 | 0.000930 | 0.003801 | 0.008354 | 0.001459 | 0.001883 | 0.002999 |
Endoplasmic Reticulum | 0.001418 | 0.002018 | 0.007175 | 0.000715 | 0.001933 | 0.001441 | 0.001071 | 0.001680 | 0.001696 | 0.001734 | 0.001642 | 0.001288 |
Endosome | 0.006267 | 0.006715 | 0.002286 | 0.007394 | 0.009735 | 0.010369 | 0.002391 | 0.002315 | 0.008137 | 0.008510 | 0.002381 | 0.004379 |
Golgi | 0.005577 | 0.005433 | 0.000559 | 0.005559 | 0.011559 | 0.005559 | 0.000743 | 0.001685 | 0.008803 | 0.005495 | 0.000726 | 0.003258 |
Lipid Particles | 0.003231 | 0.000885 | 0.003113 | 0.002685 | 0.007158 | 0.001446 | 0.000409 | 0.000926 | 0.005348 | 0.001160 | 0.000661 | 0.001640 |
Mitochondria | 0.004177 | 0.002834 | 0.000134 | 0.007114 | 0.008771 | 0.005444 | 0.001001 | 0.001522 | 0.006654 | 0.004116 | 0.000920 | 0.003794 |
Mitotic Spindle | 0.000333 | 0.002601 | 0.000541 | 0.005753 | 0.000508 | 0.002173 | 0.001182 | 0.002506 | 0.000427 | 0.002391 | 0.001122 | 0.003825 |
None | 0.000524 | 0.000220 | 0.000028 | 0.000168 | 0.000524 | 0.000277 | 0.000118 | 0.000061 | 0.000524 | 0.000248 | 0.000109 | 0.000105 |
Nuclear Periphery | 0.000017 | 0.000024 | 0.000028 | 0.000034 | 0.000021 | 0.000021 | 0.000018 | 0.000062 | 0.000019 | 0.000022 | 0.000019 | 0.000051 |
Nuclear Periphery Foci | 0.000190 | 0.000346 | 0.000787 | 0.000243 | 0.000281 | 0.000128 | 0.000303 | 0.000341 | 0.000239 | 0.000239 | 0.000348 | 0.000301 |
Nucleolus | 0.000072 | 0.000071 | 0.000007 | 0.000214 | 0.000086 | 0.000061 | 0.000018 | 0.000006 | 0.000079 | 0.000066 | 0.000017 | 0.000091 |
Nucleus | 0.004052 | 0.001785 | 0.001529 | 0.002417 | 0.002633 | 0.002847 | 0.003331 | 0.002240 | 0.003287 | 0.002307 | 0.003162 | 0.002312 |
Peroxisomes | 0.009813 | 0.012632 | 0.003585 | 0.009820 | 0.016842 | 0.017201 | 0.003542 | 0.001488 | 0.013603 | 0.014876 | 0.003546 | 0.004873 |
Vacuole | 0.010919 | 0.011124 | 0.020743 | 0.030744 | 0.006658 | 0.018132 | 0.017871 | 0.014624 | 0.008621 | 0.014565 | 0.018140 | 0.021172 |
Vacuole Periphery | 0.000352 | 0.000870 | 0.000027 | 0.000477 | 0.000618 | 0.001112 | 0.000193 | 0.000641 | 0.000495 | 0.000989 | 0.000177 | 0.000575 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -1.64 | -6.89 | -3.01 | -1.49 | 5.77 | 12.86* | 14.27 | 11.07 | -2.15 | -2.75 | 9.40 | 7.36 | 6.72 | -2.39 | -0.84 |
Bud Neck | 8.23 | 2.44 | 18.01 | 10.38 | 4.96 | -4.77 | 8.58 | 13.11 | 14.74 | 6.32 | 1.87 | 12.98 | 21.91 | 17.66 | 8.99 |
Bud Site | -5.58 | 0.83 | -4.11 | 0.84 | -2.83 | -8.41 | -22.83* | -19.81 | -9.12 | 1.96 | -10.32 | -20.08 | -17.00 | -6.17 | 3.54 |
Cell Periphery | 11.45 | 3.43 | 8.34 | -2.68 | 2.28 | 6.98 | 10.48 | 2.96 | -3.67 | -6.81 | 13.15 | 14.01 | 7.44 | -5.00 | -5.82 |
Cytoplasm | -2.67 | 7.20 | -2.90 | -0.37 | -8.66 | 0.67 | 2.59 | 1.49 | 0.67 | -1.04 | -1.33 | 2.96 | -0.86 | 0.46 | -3.49 |
Cytoplasmic Foci | 1.48 | 6.93 | 6.96 | 5.57 | -1.02 | 2.23 | 11.20 | 13.57 | 9.57 | 4.60 | 2.67 | 13.90 | 14.80 | 11.29 | 1.10 |
Eisosomes | 7.85 | -1.11 | 6.77 | -2.02 | 3.70 | 7.90 | 9.46 | 5.42 | -2.93 | -5.23 | 11.18 | 10.20 | 8.13 | -3.80 | -2.61 |
Endoplasmic Reticulum | -0.84 | -3.15 | 1.46 | 1.98 | 3.56 | 0.84 | 2.29 | 0.21 | -0.54 | -1.33 | -0.10 | -0.04 | 0.78 | 0.69 | 0.80 |
Endosome | -0.40 | 2.44 | -0.47 | -0.16 | -2.31 | -0.40 | 6.11 | 6.34 | 5.69 | 0.27 | -0.38 | 6.48 | 4.30 | 4.25 | -1.85 |
Golgi | 0.14 | 8.28 | 0.35 | 0.24 | -3.17 | 4.23 | 9.78 | 7.87 | 3.50 | -1.27 | 3.71 | 11.89 | 6.12 | 2.69 | -3.17 |
Lipid Particles | 2.68 | -0.10 | 0.46 | -1.49 | 0.41 | 4.33 | 5.28 | 4.56 | 0.69 | -1.14 | 5.13 | 5.71 | 3.91 | -0.85 | -1.62 |
Mitochondria | 1.12 | 4.37 | -1.10 | -1.81 | -3.43 | 2.28 | 8.30 | 7.24 | 3.18 | -0.88 | 2.69 | 8.26 | 3.09 | 0.59 | -3.07 |
Mitotic Spindle | -2.62 | -1.21 | -3.75 | -1.81 | -3.33 | -2.60 | -3.83 | -2.11 | -0.51 | -1.07 | -3.62 | -4.40 | -4.08 | -1.53 | -2.71 |
None | 2.22 | 4.45 | 2.70 | 0.30 | -2.59 | 1.43 | 2.99 | 3.09 | 1.55 | 0.56 | 2.50 | 4.51 | 4.15 | 1.49 | -0.35 |
Nuclear Periphery | -0.72 | -2.30 | -2.99 | -1.83 | 0.61 | 0.03 | -0.85 | -1.63 | -1.68 | -1.42 | -0.48 | -2.17 | -2.38 | -2.15 | -1.68 |
Nuclear Periphery Foci | -1.01 | -3.29 | -1.54 | 0.12 | 2.84 | 2.20 | -1.51 | -0.90 | -2.38 | 0.40 | -0.05 | -3.06 | -1.58 | -1.15 | 1.47 |
Nucleolus | 0.00 | 4.32 | -1.00 | -0.99 | -1.29 | 0.73 | 2.24 | 2.74 | 2.64 | 3.15 | 0.54 | 3.48 | -0.47 | -0.62 | -1.16 |
Nucleus | 2.82 | 0.81 | 0.04 | -3.46 | -0.87 | -0.50 | -2.41 | -0.91 | -0.35 | 1.22 | 1.62 | -1.46 | -0.53 | -2.16 | 0.95 |
Peroxisomes | -1.58 | 3.57 | 0.25 | 1.60 | -2.91 | -0.18 | 9.46 | 11.86 | 7.01 | 3.59 | -0.84 | 10.00 | 8.55 | 7.02 | -1.32 |
Vacuole | -0.18 | -3.44 | -8.83 | -8.77 | -0.97 | -5.59 | -8.29 | -6.96 | -2.37 | 0.45 | -4.54 | -8.35 | -11.05 | -8.47 | -2.76 |
Vacuole Periphery | -1.56 | 6.81 | -0.72 | 1.19 | -3.16 | -1.31 | 4.50 | 0.03 | 1.13 | -1.67 | -1.97 | 4.65 | -0.31 | 1.53 | -2.27 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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