Standard name
Human Ortholog
Description Palmitoylated vacuolar membrane-localized casein kinase I isoform; negatively regulates vacuole fusion during hypertonic stress via phosphorylation of Vps41p; shares essential functions with Hrr25p; regulates vesicle fusion in AP-3 pathway
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.15 0.11 0.15 0.23 0.26 0.27 0.22 0.24 – 0.29 0.34 0.33 0.09 0.09 0.11 0.14 0.17 0.15 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0.05 0 0 – 0 0 0 0 0 0 0.05 0.05 0 0 0 0 0 0 0
Endosome 0.18 0.07 0.49 0.37 0.15 0.07 0.09 0.08 – 0 0 0 0.18 0.16 0.18 0.09 0.13 0.11 0.22 0.21 0.3 0.26 0.27 0.26
Golgi 0 0 0 0.06 0 0 0 0 – 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Mitochondria 0 0 0 0.06 0 0.28 0.37 0.29 – 0.52 0.47 0.37 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0.05 0.05 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0.05 0 0 0 0 0 0.07 0 0.06 0.05
Nucleolus 0 0 0 0 0 0 0 0 – 0 0 0 0.06 0.06 0.05 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0.05 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.85 0.87 0.63 0.49 0.66 0.54 0.48 0.49 – 0.37 0.38 0.42 0.79 0.8 0.8 0.8 0.78 0.79 0.66 0.67 0.56 0.57 0.58 0.56
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 2 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 1 0 0 2
Bud 1 1 1 1 0 0 2 2 – 1 1 0 0 0 0 0 0 0 5 7 4 5 4 10
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 1 0 2 2
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 2 – 1 1 2 2 2 0 0 0 0 0 0 0 0 0 0
Cytoplasm 60 38 61 54 53 69 65 74 – 85 79 92 10 10 14 45 58 39 6 6 3 9 8 8
Endoplasmic Reticulum 1 5 11 9 0 14 11 8 – 12 3 3 1 1 3 15 18 9 1 0 2 2 4 5
Endosome 76 24 201 86 30 18 26 24 – 4 4 11 21 19 23 29 44 28 97 79 126 55 123 114
Golgi 0 0 4 13 6 8 1 1 – 0 0 0 1 1 2 3 4 14 3 2 2 0 0 1
Mitochondria 0 1 0 13 5 74 111 90 – 151 109 103 0 0 0 12 5 8 1 3 3 3 5 5
Nucleus 8 5 0 0 2 2 3 5 – 4 5 7 5 5 7 15 11 10 12 6 4 2 5 7
Nuclear Periphery 0 1 2 4 0 7 4 1 – 3 0 2 2 1 7 9 11 3 10 6 31 9 25 21
Nucleolus 15 9 18 7 0 1 0 3 – 1 2 0 7 7 6 5 15 8 3 2 0 0 3 6
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
SpindlePole 4 2 19 4 2 0 0 1 – 0 1 0 2 3 1 3 4 3 2 3 4 1 3 3
Vac/Vac Membrane 351 303 259 112 134 140 143 150 – 109 87 118 91 93 103 249 261 208 288 251 236 124 265 252
Unique Cell Count 413 348 414 230 203 260 298 309 291 231 281 115 116 129 313 334 264 436 375 425 219 455 449
Labelled Cell Count 516 389 578 303 232 333 366 361 371 292 338 142 142 166 385 431 330 436 375 425 219 455 449


Vacuole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 9.8 9.0 8.6 8.1 8.0 6.3 6.2 6.4 – 5.7 5.8 6.1 9.9 9.9 9.7 8.7 10.0 10.3 9.9 10.1 10.6
Std Deviation (1e-4) 1.3 1.4 1.2 1.4 1.4 1.1 1.5 1.5 – 1.2 1.1 1.4 1.3 1.3 1.6 1.7 2.2 2.3 2.0 1.6 2.1
Intensity Change (Log2) – – – -0.08 -0.1 -0.44 -0.46 -0.43 – -0.58 -0.57 -0.5 0.21 0.21 0.18 0.01 0.22 0.26 0.21 0.24 0.3

WT3RAP60RAP140RAP220RAP300RAP380RAP540RAP620RAP7000510WT3HU80HU120HU1600510WT3rpd3Δ_1rpd3Δ_2rpd3Δ_30510WT1AF100AF140AF1800510
rapamycinhydroxy-urearpd3 knockoutalpha-factorMultiple Condition Time-Course Intensity PlotsGFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cytoplasm 2.8 3.4 3.8 2.4 3.1 – 4.7 5.7 5.6 -1.7 -1.7 -1.1 -0.1 1.0 0
Endoplasmic Reticulum 0.9 0 1.8 0.8 -0.1 – 1.1 -1.1 -1.5 0 0 0 1.5 1.9 0.6
Endosome -2.7 -8.1 -11.2 -11.2 -11.7 – -13.6 -12.2 -12.5 -5.8 -6.2 -6.2 -11.3 -10.2 -10.2
Golgi 3.6 0 0 0 0 – 0 0 0 0 0 0 0 0 3.4
Mitochondria 0 0 11.5 13.5 11.7 – 16.5 15.3 13.3 0 0 0 4.0 0 0
Nucleus 0 0 0 0 0 – 0 0 0 0 0 0 4.5 0 0
Nuclear Periphery 0 0 0 0 0 – 0 0 0 0 0 0 0 2.9 0
Nucleolus -0.8 -3.0 -3.0 -3.6 -2.7 – -3.2 -2.4 -3.5 0.8 0.8 0.1 -2.1 0.1 -0.9
Peroxisomes 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
SpindlePole -1.9 -2.3 -3.5 -3.7 -3.5 – -3.7 -2.9 -3.6 0 0 0 -2.8 -2.7 -2.5
Vacuole -3.4 0.8 -2.2 -3.9 -3.8 – -6.6 -6.1 -5.3 3.3 3.5 3.6 4.9 4.6 4.5

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 7.0277 6.3403 5.9014 5.8189 5.8536 6.4771 8.4626 9.5571 8.8926 8.237 8.9274 8.9861 8.6093 10.1935 9.5741 9.284 8.9746 9.4157
Actin 0.0117 0.0002 0.0008 0.0001 0 0.0002 0.0257 0 0.013 0.0252 0.0037 0.0055 0 0 0 0 0 0
Bud 0.0013 0.0035 0.0021 0.0002 0.0005 0.0053 0.001 0.0004 0.0017 0.0004 0.0004 0.0004 0.0005 0.0002 0.0004 0.0002 0.0004 0.0003
Bud Neck 0.0012 0.0007 0.0025 0.0009 0.0003 0.0038 0.0012 0.0008 0.0012 0.0002 0.0005 0.0014 0.0007 0.0004 0.0029 0.0001 0.0005 0.0008
Bud Periphery 0.0021 0.0027 0.0017 0.0002 0.0004 0.0034 0.0014 0.0003 0.0016 0.0003 0.0002 0.0004 0.0007 0.0002 0.0005 0.0002 0.0002 0.0004
Bud Site 0.0014 0.0076 0.0056 0.0005 0.0007 0.0032 0.0015 0.0017 0.0048 0.0009 0.0004 0.0006 0.0032 0.0006 0.0013 0.0001 0.0011 0.0005
Cell Periphery 0.0007 0.0006 0.0005 0.0002 0.0002 0.0028 0.0005 0.0004 0.0004 0.0001 0.0001 0.0001 0.0004 0.0003 0.0003 0.0001 0.0001 0.0006
Cytoplasm 0.0248 0.0898 0.0644 0.0347 0.0203 0.0379 0.0723 0.099 0.1278 0.0722 0.0732 0.1059 0.0151 0.0292 0.0184 0.0123 0.0113 0.021
Cytoplasmic Foci 0.0196 0.0196 0.0207 0.0264 0.0132 0.0264 0.0364 0.0119 0.0305 0.0116 0.0171 0.0333 0.0096 0.0062 0.01 0.008 0.0073 0.013
Eisosomes 0.0001 0 0 0 0 0 0.0002 0 0.0001 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0.0074 0.004 0.0053 0.005 0.0011 0.0096 0.0147 0.0107 0.0086 0.0022 0.0037 0.0065 0.005 0.0098 0.0059 0.0033 0.0016 0.0041
Endosome 0.261 0.1543 0.2304 0.2141 0.2201 0.2108 0.3375 0.1808 0.289 0.344 0.283 0.2998 0.2734 0.1797 0.2375 0.298 0.2303 0.2141
Golgi 0.0055 0.0021 0.0043 0.0057 0.0023 0.0033 0.0193 0.0016 0.0144 0.0044 0.0059 0.0142 0.0033 0.0018 0.0047 0.0017 0.0023 0.0038
Lipid Particles 0.0068 0.0022 0.0039 0.0083 0.0004 0.0018 0.0166 0.0006 0.0038 0.0009 0.0154 0.0036 0.0124 0.0024 0.0017 0.0017 0.0011 0.003
Mitochondria 0.0213 0.0027 0.0038 0.0096 0.0003 0.0012 0.0238 0.0005 0.0153 0.0021 0.0008 0.0053 0.0014 0.0004 0.0018 0.0024 0.0002 0.0007
None 0.0412 0.0238 0.0561 0.0012 0.0001 0.0063 0.0029 0.0004 0.0014 0.0009 0.0007 0.0008 0.0002 0.0001 0.0001 0.0001 0.0001 0.0002
Nuclear Periphery 0.0257 0.0303 0.0151 0.0147 0.0058 0.0353 0.0194 0.0366 0.0124 0.0059 0.0072 0.0179 0.0236 0.0289 0.0349 0.0151 0.0058 0.0159
Nucleolus 0.0093 0.0034 0.0035 0.0007 0.0005 0.0013 0.0008 0.0018 0.0004 0.0003 0.0004 0.0007 0.0038 0.0085 0.0022 0.0002 0.0004 0.0018
Nucleus 0.0156 0.0267 0.017 0.0196 0.0021 0.0163 0.0255 0.0082 0.0054 0.0021 0.003 0.0099 0.0035 0.004 0.0043 0.0014 0.0025 0.0046
Peroxisomes 0.0043 0.0014 0.0014 0.0162 0.0003 0.0005 0.004 0.0001 0.0073 0.0033 0.0071 0.0018 0.0004 0.0001 0.0002 0 0.0001 0.0003
Punctate Nuclear 0.0093 0.0012 0.003 0.0007 0.0001 0.0065 0.0015 0.0002 0.0008 0.0028 0.0007 0.0018 0.0003 0.0005 0.0005 0 0.0001 0.002
Vacuole 0.4362 0.5104 0.417 0.4772 0.4871 0.4506 0.312 0.5236 0.377 0.3778 0.4109 0.3767 0.4803 0.4793 0.4253 0.4152 0.5103 0.4782
Vacuole Periphery 0.0934 0.1129 0.1408 0.164 0.2442 0.1738 0.0817 0.1205 0.0832 0.1422 0.1654 0.1132 0.1624 0.2472 0.2469 0.2399 0.2245 0.2347

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 16.1132 19.4144 15.3358 25.9629 19.529 10.5028 22.6377 20.9417 21.7513 27.86
Translational Efficiency 1.2816 1.0625 1.2713 0.8359 1.0066 1.9649 1.0097 1.064 1.0545 0.8955

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
190 1407 1385 1013 2073 1126 263 43 2263 2533 1648 1056

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 955.13 1026.87 1206.30 1074.33 888.60 999.68 1189.18 1182.14 894.19 1014.78 1203.57 1078.72
Standard Deviation 123.23 176.56 179.89 149.81 133.21 145.94 130.89 151.54 133.68 164.21 173.12 151.39
Intensity Change Log 2 — 0.104485 0.336820 0.169668 — 0.169932 0.420361 0.411795 — 0.136398 0.377687 0.291440

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000743 0.000077 0.000065 0.000232 0.000008 0.000250 0.000132 0.000039 0.000070 0.000154 0.000076 0.000224
Bud Neck 0.016288 0.002975 0.005673 0.011251 0.000985 0.005564 0.025762 0.007407 0.002270 0.004126 0.008879 0.011095
Bud Site 0.027997 0.013145 0.005183 0.024900 0.004379 0.009558 0.023945 0.018740 0.006362 0.011550 0.008177 0.024649
Cell Periphery 0.000156 0.000093 0.000076 0.000188 0.000150 0.000087 0.000086 0.000064 0.000151 0.000090 0.000077 0.000183
Cytoplasm 0.077602 0.022448 0.004696 0.014622 0.009424 0.019116 0.008524 0.005706 0.015148 0.020967 0.005307 0.014259
Cytoplasmic Foci 0.027268 0.017137 0.004632 0.007777 0.008126 0.029349 0.009219 0.009335 0.009733 0.022566 0.005364 0.007840
Eisosomes 0.000020 0.000007 0.000013 0.000013 0.000003 0.000014 0.000030 0.000011 0.000005 0.000010 0.000016 0.000013
Endoplasmic Reticulum 0.001131 0.001433 0.000801 0.001607 0.003053 0.001773 0.001718 0.001778 0.002891 0.001584 0.000948 0.001613
Endosome 0.076443 0.067150 0.020611 0.053198 0.027108 0.131295 0.057995 0.090398 0.031250 0.095664 0.026577 0.054712
Golgi 0.009295 0.006704 0.006091 0.009769 0.001695 0.011623 0.005442 0.001461 0.002333 0.008891 0.005987 0.009431
Lipid Particles 0.000676 0.000348 0.000262 0.000493 0.000215 0.001815 0.000493 0.001578 0.000254 0.001000 0.000299 0.000537
Mitochondria 0.000930 0.002339 0.002019 0.006849 0.001520 0.004328 0.000780 0.000251 0.001471 0.003223 0.001821 0.006581
Mitotic Spindle 0.009743 0.003281 0.006267 0.007084 0.000071 0.005935 0.006377 0.006950 0.000883 0.004461 0.006285 0.007079
None 0.000241 0.000384 0.000237 0.000121 0.000110 0.000446 0.000187 0.000049 0.000121 0.000412 0.000229 0.000118
Nuclear Periphery 0.000499 0.000784 0.005422 0.001647 0.000290 0.001137 0.001008 0.001098 0.000308 0.000941 0.004717 0.001625
Nuclear Periphery Foci 0.000806 0.001033 0.004200 0.002088 0.000205 0.003461 0.001098 0.008070 0.000256 0.002113 0.003705 0.002332
Nucleolus 0.003581 0.001760 0.014364 0.002894 0.000248 0.004873 0.022557 0.007549 0.000527 0.003144 0.015671 0.003083
Nucleus 0.014134 0.003777 0.012301 0.005177 0.000740 0.007180 0.031289 0.008978 0.001864 0.005290 0.015331 0.005332
Peroxisomes 0.001830 0.001382 0.001148 0.001162 0.000195 0.002463 0.001745 0.005277 0.000332 0.001863 0.001243 0.001330
Vacuole 0.698523 0.454114 0.358472 0.695053 0.476294 0.479252 0.615469 0.716330 0.494953 0.465289 0.399486 0.695919
Vacuole Periphery 0.032093 0.399629 0.547466 0.153876 0.465180 0.280480 0.186144 0.108930 0.428818 0.346663 0.489804 0.152046

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 1.06 1.07 0.77 -1.68 -1.86 -1.84 -3.57 -1.99 1.57 2.53 -1.23 -0.62 -1.79 -0.66 -1.68
Bud Neck 4.83 3.80 1.70 -6.89 -4.41 -6.70 -6.36 -2.75 -0.78 4.28 -3.70 -8.19 -7.51 -6.12 -1.61
Bud Site 2.43 3.75 0.49 -4.57 -8.56 -4.92 -4.86 -1.98 -1.25 0.66 -4.74 -2.06 -8.12 -5.68 -7.09
Cell Periphery 0.54 0.75 -0.58 -3.45 -4.15 3.69 3.86 3.70 1.04 1.01 3.88 4.89 -1.00 -3.52 -4.08
Cytoplasm 6.45 8.18 6.56 0.42 -6.14 -3.62 -0.69 0.69 3.41 1.07 -2.10 5.59 -1.57 0.15 -5.77
Cytoplasmic Foci 3.09 6.97 5.89 6.43 -3.54 -9.59 -0.52 -0.49 5.25 -0.13 -9.44 4.90 1.64 10.53 -2.85
Eisosomes 1.95 0.93 1.02 -4.54 0.44 -5.44 -11.07 -2.67 0.93 4.90 -4.91 -10.63 -6.40 -1.69 2.34
Endoplasmic Reticulum -1.09 1.18 -1.57 -0.54 -3.72 3.02 3.19 1.98 -0.19 -0.14 3.63 5.56 3.53 -0.01 -3.18
Endosome 0.46 6.97 2.56 3.62 -9.99 -17.74 -5.54 -3.28 2.10 -1.67 -18.13 1.87 -7.10 10.16 -8.54
Golgi 0.22 0.36 -0.30 -1.69 -2.15 -6.48 -2.59 0.63 6.53 2.71 -7.80 -5.92 -4.88 0.02 -2.10
Lipid Particles 4.50 5.73 2.28 -2.44 -3.93 -2.48 -3.80 -1.35 -0.07 -1.05 -2.57 -1.74 -3.94 1.35 -3.27
Mitochondria -2.94 -3.36 -3.44 -2.47 -2.69 -4.59 3.41 7.03 6.89 3.89 -4.59 -1.70 -3.06 -1.88 -2.78
Mitotic Spindle 1.80 1.26 1.09 -2.45 -0.49 -4.51 -2.33 -1.34 -0.46 -0.18 -3.54 -4.50 -3.80 -1.87 -0.45
None -1.04 -0.03 0.98 2.89 2.82 -3.63 -1.48 1.45 4.33 2.63 -4.42 -3.11 -0.44 4.28 2.91
Nuclear Periphery -0.79 -6.32 -1.95 -1.53 3.99 -3.62 -4.75 -1.61 -0.15 -0.11 -3.40 -6.82 -2.49 -1.34 3.71
Nuclear Periphery Foci -0.33 -4.92 -1.70 -1.56 2.55 -3.37 -2.83 -1.12 -0.74 -0.99 -4.09 -6.59 -3.13 -0.31 1.66
Nucleolus 2.69 -6.84 0.85 -2.82 7.80 -5.85 -6.73 -1.51 -0.49 2.76 -6.74 -11.67 -6.26 -0.20 9.18
Nucleus 4.52 0.96 3.58 -2.53 4.41 -6.53 -6.11 -1.68 -0.31 3.51 -5.18 -9.63 -5.32 -1.21 6.13
Peroxisomes 0.92 1.73 1.79 0.53 -0.05 -5.47 -5.42 -1.07 -0.54 -0.72 -5.63 -4.09 -3.50 1.51 -0.21
Vacuole 11.11 15.83 -0.78 -24.73 -35.05 -0.21 -9.07 -6.63 -6.49 -2.68 3.94 11.64 -22.62 -26.39 -31.47
Vacuole Periphery -44.92 -61.13 -18.58 27.44 42.32 19.92 22.42 19.92 9.28 3.48 10.48 -5.67 36.50 26.84 37.35
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Components••••••••••••••••actinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
Standard name
Human Ortholog
Description Palmitoylated vacuolar membrane-localized casein kinase I isoform; negatively regulates vacuole fusion during hypertonic stress via phosphorylation of Vps41p; shares essential functions with Hrr25p; regulates vesicle fusion in AP-3 pathway
Localization
Cell Percentages vacuolar membrane (82%)
Cell Cycle Regulation No
Subcompartmental Group vac memb-1

Yck3

Yck3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Yck3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available