Standard name | |
---|---|
Human Ortholog | |
Description | Palmitoylated vacuolar membrane-localized casein kinase I isoform; negatively regulates vacuole fusion during hypertonic stress via phosphorylation of Vps41p; shares essential functions with Hrr25p; regulates vesicle fusion in AP-3 pathway |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 2 |
Bud | 1 | 1 | 1 | 1 | 0 | 0 | 2 | 2 | – | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 5 | 7 | 4 | 5 | 4 | 10 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 2 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | – | 1 | 1 | 2 | 2 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 60 | 38 | 61 | 54 | 53 | 69 | 65 | 74 | – | 85 | 79 | 92 | 10 | 10 | 14 | 45 | 58 | 39 | 6 | 6 | 3 | 9 | 8 | 8 |
Endoplasmic Reticulum | 1 | 5 | 11 | 9 | 0 | 14 | 11 | 8 | – | 12 | 3 | 3 | 1 | 1 | 3 | 15 | 18 | 9 | 1 | 0 | 2 | 2 | 4 | 5 |
Endosome | 76 | 24 | 201 | 86 | 30 | 18 | 26 | 24 | – | 4 | 4 | 11 | 21 | 19 | 23 | 29 | 44 | 28 | 97 | 79 | 126 | 55 | 123 | 114 |
Golgi | 0 | 0 | 4 | 13 | 6 | 8 | 1 | 1 | – | 0 | 0 | 0 | 1 | 1 | 2 | 3 | 4 | 14 | 3 | 2 | 2 | 0 | 0 | 1 |
Mitochondria | 0 | 1 | 0 | 13 | 5 | 74 | 111 | 90 | – | 151 | 109 | 103 | 0 | 0 | 0 | 12 | 5 | 8 | 1 | 3 | 3 | 3 | 5 | 5 |
Nucleus | 8 | 5 | 0 | 0 | 2 | 2 | 3 | 5 | – | 4 | 5 | 7 | 5 | 5 | 7 | 15 | 11 | 10 | 12 | 6 | 4 | 2 | 5 | 7 |
Nuclear Periphery | 0 | 1 | 2 | 4 | 0 | 7 | 4 | 1 | – | 3 | 0 | 2 | 2 | 1 | 7 | 9 | 11 | 3 | 10 | 6 | 31 | 9 | 25 | 21 |
Nucleolus | 15 | 9 | 18 | 7 | 0 | 1 | 0 | 3 | – | 1 | 2 | 0 | 7 | 7 | 6 | 5 | 15 | 8 | 3 | 2 | 0 | 0 | 3 | 6 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
SpindlePole | 4 | 2 | 19 | 4 | 2 | 0 | 0 | 1 | – | 0 | 1 | 0 | 2 | 3 | 1 | 3 | 4 | 3 | 2 | 3 | 4 | 1 | 3 | 3 |
Vac/Vac Membrane | 351 | 303 | 259 | 112 | 134 | 140 | 143 | 150 | – | 109 | 87 | 118 | 91 | 93 | 103 | 249 | 261 | 208 | 288 | 251 | 236 | 124 | 265 | 252 |
Unique Cell Count | 413 | 348 | 414 | 230 | 203 | 260 | 298 | 309 | 291 | 231 | 281 | 115 | 116 | 129 | 313 | 334 | 264 | 436 | 375 | 425 | 219 | 455 | 449 | |
Labelled Cell Count | 516 | 389 | 578 | 303 | 232 | 333 | 366 | 361 | 371 | 292 | 338 | 142 | 142 | 166 | 385 | 431 | 330 | 436 | 375 | 425 | 219 | 455 | 449 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 9.8 | 9.0 | 8.6 | 8.1 | 8.0 | 6.3 | 6.2 | 6.4 | – | 5.7 | 5.8 | 6.1 | 9.9 | 9.9 | 9.7 | 8.7 | 10.0 | 10.3 | 9.9 | 10.1 | 10.6 |
Std Deviation (1e-4) | 1.3 | 1.4 | 1.2 | 1.4 | 1.4 | 1.1 | 1.5 | 1.5 | – | 1.2 | 1.1 | 1.4 | 1.3 | 1.3 | 1.6 | 1.7 | 2.2 | 2.3 | 2.0 | 1.6 | 2.1 |
Intensity Change (Log2) | – | – | – | -0.08 | -0.1 | -0.44 | -0.46 | -0.43 | – | -0.58 | -0.57 | -0.5 | 0.21 | 0.21 | 0.18 | 0.01 | 0.22 | 0.26 | 0.21 | 0.24 | 0.3 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 2.8 | 3.4 | 3.8 | 2.4 | 3.1 | – | 4.7 | 5.7 | 5.6 | -1.7 | -1.7 | -1.1 | -0.1 | 1.0 | 0 |
Endoplasmic Reticulum | 0.9 | 0 | 1.8 | 0.8 | -0.1 | – | 1.1 | -1.1 | -1.5 | 0 | 0 | 0 | 1.5 | 1.9 | 0.6 |
Endosome | -2.7 | -8.1 | -11.2 | -11.2 | -11.7 | – | -13.6 | -12.2 | -12.5 | -5.8 | -6.2 | -6.2 | -11.3 | -10.2 | -10.2 |
Golgi | 3.6 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.4 |
Mitochondria | 0 | 0 | 11.5 | 13.5 | 11.7 | – | 16.5 | 15.3 | 13.3 | 0 | 0 | 0 | 4.0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 4.5 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.9 | 0 |
Nucleolus | -0.8 | -3.0 | -3.0 | -3.6 | -2.7 | – | -3.2 | -2.4 | -3.5 | 0.8 | 0.8 | 0.1 | -2.1 | 0.1 | -0.9 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | -1.9 | -2.3 | -3.5 | -3.7 | -3.5 | – | -3.7 | -2.9 | -3.6 | 0 | 0 | 0 | -2.8 | -2.7 | -2.5 |
Vacuole | -3.4 | 0.8 | -2.2 | -3.9 | -3.8 | – | -6.6 | -6.1 | -5.3 | 3.3 | 3.5 | 3.6 | 4.9 | 4.6 | 4.5 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 7.0277 | 6.3403 | 5.9014 | 5.8189 | 5.8536 | 6.4771 | 8.4626 | 9.5571 | 8.8926 | 8.237 | 8.9274 | 8.9861 | 8.6093 | 10.1935 | 9.5741 | 9.284 | 8.9746 | 9.4157 |
Actin | 0.0117 | 0.0002 | 0.0008 | 0.0001 | 0 | 0.0002 | 0.0257 | 0 | 0.013 | 0.0252 | 0.0037 | 0.0055 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0.0013 | 0.0035 | 0.0021 | 0.0002 | 0.0005 | 0.0053 | 0.001 | 0.0004 | 0.0017 | 0.0004 | 0.0004 | 0.0004 | 0.0005 | 0.0002 | 0.0004 | 0.0002 | 0.0004 | 0.0003 |
Bud Neck | 0.0012 | 0.0007 | 0.0025 | 0.0009 | 0.0003 | 0.0038 | 0.0012 | 0.0008 | 0.0012 | 0.0002 | 0.0005 | 0.0014 | 0.0007 | 0.0004 | 0.0029 | 0.0001 | 0.0005 | 0.0008 |
Bud Periphery | 0.0021 | 0.0027 | 0.0017 | 0.0002 | 0.0004 | 0.0034 | 0.0014 | 0.0003 | 0.0016 | 0.0003 | 0.0002 | 0.0004 | 0.0007 | 0.0002 | 0.0005 | 0.0002 | 0.0002 | 0.0004 |
Bud Site | 0.0014 | 0.0076 | 0.0056 | 0.0005 | 0.0007 | 0.0032 | 0.0015 | 0.0017 | 0.0048 | 0.0009 | 0.0004 | 0.0006 | 0.0032 | 0.0006 | 0.0013 | 0.0001 | 0.0011 | 0.0005 |
Cell Periphery | 0.0007 | 0.0006 | 0.0005 | 0.0002 | 0.0002 | 0.0028 | 0.0005 | 0.0004 | 0.0004 | 0.0001 | 0.0001 | 0.0001 | 0.0004 | 0.0003 | 0.0003 | 0.0001 | 0.0001 | 0.0006 |
Cytoplasm | 0.0248 | 0.0898 | 0.0644 | 0.0347 | 0.0203 | 0.0379 | 0.0723 | 0.099 | 0.1278 | 0.0722 | 0.0732 | 0.1059 | 0.0151 | 0.0292 | 0.0184 | 0.0123 | 0.0113 | 0.021 |
Cytoplasmic Foci | 0.0196 | 0.0196 | 0.0207 | 0.0264 | 0.0132 | 0.0264 | 0.0364 | 0.0119 | 0.0305 | 0.0116 | 0.0171 | 0.0333 | 0.0096 | 0.0062 | 0.01 | 0.008 | 0.0073 | 0.013 |
Eisosomes | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0.0002 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0074 | 0.004 | 0.0053 | 0.005 | 0.0011 | 0.0096 | 0.0147 | 0.0107 | 0.0086 | 0.0022 | 0.0037 | 0.0065 | 0.005 | 0.0098 | 0.0059 | 0.0033 | 0.0016 | 0.0041 |
Endosome | 0.261 | 0.1543 | 0.2304 | 0.2141 | 0.2201 | 0.2108 | 0.3375 | 0.1808 | 0.289 | 0.344 | 0.283 | 0.2998 | 0.2734 | 0.1797 | 0.2375 | 0.298 | 0.2303 | 0.2141 |
Golgi | 0.0055 | 0.0021 | 0.0043 | 0.0057 | 0.0023 | 0.0033 | 0.0193 | 0.0016 | 0.0144 | 0.0044 | 0.0059 | 0.0142 | 0.0033 | 0.0018 | 0.0047 | 0.0017 | 0.0023 | 0.0038 |
Lipid Particles | 0.0068 | 0.0022 | 0.0039 | 0.0083 | 0.0004 | 0.0018 | 0.0166 | 0.0006 | 0.0038 | 0.0009 | 0.0154 | 0.0036 | 0.0124 | 0.0024 | 0.0017 | 0.0017 | 0.0011 | 0.003 |
Mitochondria | 0.0213 | 0.0027 | 0.0038 | 0.0096 | 0.0003 | 0.0012 | 0.0238 | 0.0005 | 0.0153 | 0.0021 | 0.0008 | 0.0053 | 0.0014 | 0.0004 | 0.0018 | 0.0024 | 0.0002 | 0.0007 |
None | 0.0412 | 0.0238 | 0.0561 | 0.0012 | 0.0001 | 0.0063 | 0.0029 | 0.0004 | 0.0014 | 0.0009 | 0.0007 | 0.0008 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0002 |
Nuclear Periphery | 0.0257 | 0.0303 | 0.0151 | 0.0147 | 0.0058 | 0.0353 | 0.0194 | 0.0366 | 0.0124 | 0.0059 | 0.0072 | 0.0179 | 0.0236 | 0.0289 | 0.0349 | 0.0151 | 0.0058 | 0.0159 |
Nucleolus | 0.0093 | 0.0034 | 0.0035 | 0.0007 | 0.0005 | 0.0013 | 0.0008 | 0.0018 | 0.0004 | 0.0003 | 0.0004 | 0.0007 | 0.0038 | 0.0085 | 0.0022 | 0.0002 | 0.0004 | 0.0018 |
Nucleus | 0.0156 | 0.0267 | 0.017 | 0.0196 | 0.0021 | 0.0163 | 0.0255 | 0.0082 | 0.0054 | 0.0021 | 0.003 | 0.0099 | 0.0035 | 0.004 | 0.0043 | 0.0014 | 0.0025 | 0.0046 |
Peroxisomes | 0.0043 | 0.0014 | 0.0014 | 0.0162 | 0.0003 | 0.0005 | 0.004 | 0.0001 | 0.0073 | 0.0033 | 0.0071 | 0.0018 | 0.0004 | 0.0001 | 0.0002 | 0 | 0.0001 | 0.0003 |
Punctate Nuclear | 0.0093 | 0.0012 | 0.003 | 0.0007 | 0.0001 | 0.0065 | 0.0015 | 0.0002 | 0.0008 | 0.0028 | 0.0007 | 0.0018 | 0.0003 | 0.0005 | 0.0005 | 0 | 0.0001 | 0.002 |
Vacuole | 0.4362 | 0.5104 | 0.417 | 0.4772 | 0.4871 | 0.4506 | 0.312 | 0.5236 | 0.377 | 0.3778 | 0.4109 | 0.3767 | 0.4803 | 0.4793 | 0.4253 | 0.4152 | 0.5103 | 0.4782 |
Vacuole Periphery | 0.0934 | 0.1129 | 0.1408 | 0.164 | 0.2442 | 0.1738 | 0.0817 | 0.1205 | 0.0832 | 0.1422 | 0.1654 | 0.1132 | 0.1624 | 0.2472 | 0.2469 | 0.2399 | 0.2245 | 0.2347 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 16.1132 | 19.4144 | 15.3358 | 25.9629 | 19.529 | 10.5028 | 22.6377 | 20.9417 | 21.7513 | 27.86 |
Translational Efficiency | 1.2816 | 1.0625 | 1.2713 | 0.8359 | 1.0066 | 1.9649 | 1.0097 | 1.064 | 1.0545 | 0.8955 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
190 | 1407 | 1385 | 1013 | 2073 | 1126 | 263 | 43 | 2263 | 2533 | 1648 | 1056 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 955.13 | 1026.87 | 1206.30 | 1074.33 | 888.60 | 999.68 | 1189.18 | 1182.14 | 894.19 | 1014.78 | 1203.57 | 1078.72 |
Standard Deviation | 123.23 | 176.56 | 179.89 | 149.81 | 133.21 | 145.94 | 130.89 | 151.54 | 133.68 | 164.21 | 173.12 | 151.39 |
Intensity Change Log 2 | — | 0.104485 | 0.336820 | 0.169668 | — | 0.169932 | 0.420361 | 0.411795 | — | 0.136398 | 0.377687 | 0.291440 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000743 | 0.000077 | 0.000065 | 0.000232 | 0.000008 | 0.000250 | 0.000132 | 0.000039 | 0.000070 | 0.000154 | 0.000076 | 0.000224 |
Bud Neck | 0.016288 | 0.002975 | 0.005673 | 0.011251 | 0.000985 | 0.005564 | 0.025762 | 0.007407 | 0.002270 | 0.004126 | 0.008879 | 0.011095 |
Bud Site | 0.027997 | 0.013145 | 0.005183 | 0.024900 | 0.004379 | 0.009558 | 0.023945 | 0.018740 | 0.006362 | 0.011550 | 0.008177 | 0.024649 |
Cell Periphery | 0.000156 | 0.000093 | 0.000076 | 0.000188 | 0.000150 | 0.000087 | 0.000086 | 0.000064 | 0.000151 | 0.000090 | 0.000077 | 0.000183 |
Cytoplasm | 0.077602 | 0.022448 | 0.004696 | 0.014622 | 0.009424 | 0.019116 | 0.008524 | 0.005706 | 0.015148 | 0.020967 | 0.005307 | 0.014259 |
Cytoplasmic Foci | 0.027268 | 0.017137 | 0.004632 | 0.007777 | 0.008126 | 0.029349 | 0.009219 | 0.009335 | 0.009733 | 0.022566 | 0.005364 | 0.007840 |
Eisosomes | 0.000020 | 0.000007 | 0.000013 | 0.000013 | 0.000003 | 0.000014 | 0.000030 | 0.000011 | 0.000005 | 0.000010 | 0.000016 | 0.000013 |
Endoplasmic Reticulum | 0.001131 | 0.001433 | 0.000801 | 0.001607 | 0.003053 | 0.001773 | 0.001718 | 0.001778 | 0.002891 | 0.001584 | 0.000948 | 0.001613 |
Endosome | 0.076443 | 0.067150 | 0.020611 | 0.053198 | 0.027108 | 0.131295 | 0.057995 | 0.090398 | 0.031250 | 0.095664 | 0.026577 | 0.054712 |
Golgi | 0.009295 | 0.006704 | 0.006091 | 0.009769 | 0.001695 | 0.011623 | 0.005442 | 0.001461 | 0.002333 | 0.008891 | 0.005987 | 0.009431 |
Lipid Particles | 0.000676 | 0.000348 | 0.000262 | 0.000493 | 0.000215 | 0.001815 | 0.000493 | 0.001578 | 0.000254 | 0.001000 | 0.000299 | 0.000537 |
Mitochondria | 0.000930 | 0.002339 | 0.002019 | 0.006849 | 0.001520 | 0.004328 | 0.000780 | 0.000251 | 0.001471 | 0.003223 | 0.001821 | 0.006581 |
Mitotic Spindle | 0.009743 | 0.003281 | 0.006267 | 0.007084 | 0.000071 | 0.005935 | 0.006377 | 0.006950 | 0.000883 | 0.004461 | 0.006285 | 0.007079 |
None | 0.000241 | 0.000384 | 0.000237 | 0.000121 | 0.000110 | 0.000446 | 0.000187 | 0.000049 | 0.000121 | 0.000412 | 0.000229 | 0.000118 |
Nuclear Periphery | 0.000499 | 0.000784 | 0.005422 | 0.001647 | 0.000290 | 0.001137 | 0.001008 | 0.001098 | 0.000308 | 0.000941 | 0.004717 | 0.001625 |
Nuclear Periphery Foci | 0.000806 | 0.001033 | 0.004200 | 0.002088 | 0.000205 | 0.003461 | 0.001098 | 0.008070 | 0.000256 | 0.002113 | 0.003705 | 0.002332 |
Nucleolus | 0.003581 | 0.001760 | 0.014364 | 0.002894 | 0.000248 | 0.004873 | 0.022557 | 0.007549 | 0.000527 | 0.003144 | 0.015671 | 0.003083 |
Nucleus | 0.014134 | 0.003777 | 0.012301 | 0.005177 | 0.000740 | 0.007180 | 0.031289 | 0.008978 | 0.001864 | 0.005290 | 0.015331 | 0.005332 |
Peroxisomes | 0.001830 | 0.001382 | 0.001148 | 0.001162 | 0.000195 | 0.002463 | 0.001745 | 0.005277 | 0.000332 | 0.001863 | 0.001243 | 0.001330 |
Vacuole | 0.698523* | 0.454114* | 0.358472* | 0.695053* | 0.476294* | 0.479252* | 0.615469* | 0.716330* | 0.494953* | 0.465289* | 0.399486* | 0.695919* |
Vacuole Periphery | 0.032093 | 0.399629* | 0.547466* | 0.153876* | 0.465180* | 0.280480* | 0.186144* | 0.108930* | 0.428818* | 0.346663* | 0.489804* | 0.152046* |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 1.06 | 1.07 | 0.77 | -1.68 | -1.86 | -1.84 | -3.57 | -1.99 | 1.57 | 2.53 | -1.23 | -0.62 | -1.79 | -0.66 | -1.68 |
Bud Neck | 4.83 | 3.80 | 1.70 | -6.89 | -4.41 | -6.70 | -6.36 | -2.75 | -0.78 | 4.28 | -3.70 | -8.19 | -7.51 | -6.12 | -1.61 |
Bud Site | 2.43 | 3.75 | 0.49 | -4.57 | -8.56 | -4.92 | -4.86 | -1.98 | -1.25 | 0.66 | -4.74 | -2.06 | -8.12 | -5.68 | -7.09 |
Cell Periphery | 0.54 | 0.75 | -0.58 | -3.45 | -4.15 | 3.69 | 3.86 | 3.70 | 1.04 | 1.01 | 3.88 | 4.89 | -1.00 | -3.52 | -4.08 |
Cytoplasm | 6.45 | 8.18 | 6.56 | 0.42 | -6.14 | -3.62 | -0.69 | 0.69 | 3.41 | 1.07 | -2.10 | 5.59 | -1.57 | 0.15 | -5.77 |
Cytoplasmic Foci | 3.09 | 6.97 | 5.89 | 6.43 | -3.54 | -9.59 | -0.52 | -0.49 | 5.25 | -0.13 | -9.44 | 4.90 | 1.64 | 10.53 | -2.85 |
Eisosomes | 1.95 | 0.93 | 1.02 | -4.54 | 0.44 | -5.44 | -11.07 | -2.67 | 0.93 | 4.90 | -4.91 | -10.63 | -6.40 | -1.69 | 2.34 |
Endoplasmic Reticulum | -1.09 | 1.18 | -1.57 | -0.54 | -3.72 | 3.02 | 3.19 | 1.98 | -0.19 | -0.14 | 3.63 | 5.56 | 3.53 | -0.01 | -3.18 |
Endosome | 0.46 | 6.97 | 2.56 | 3.62 | -9.99 | -17.74 | -5.54 | -3.28 | 2.10 | -1.67 | -18.13 | 1.87 | -7.10 | 10.16 | -8.54 |
Golgi | 0.22 | 0.36 | -0.30 | -1.69 | -2.15 | -6.48 | -2.59 | 0.63 | 6.53 | 2.71 | -7.80 | -5.92 | -4.88 | 0.02 | -2.10 |
Lipid Particles | 4.50 | 5.73 | 2.28 | -2.44 | -3.93 | -2.48 | -3.80 | -1.35 | -0.07 | -1.05 | -2.57 | -1.74 | -3.94 | 1.35 | -3.27 |
Mitochondria | -2.94 | -3.36 | -3.44 | -2.47 | -2.69 | -4.59 | 3.41 | 7.03 | 6.89 | 3.89 | -4.59 | -1.70 | -3.06 | -1.88 | -2.78 |
Mitotic Spindle | 1.80 | 1.26 | 1.09 | -2.45 | -0.49 | -4.51 | -2.33 | -1.34 | -0.46 | -0.18 | -3.54 | -4.50 | -3.80 | -1.87 | -0.45 |
None | -1.04 | -0.03 | 0.98 | 2.89 | 2.82 | -3.63 | -1.48 | 1.45 | 4.33 | 2.63 | -4.42 | -3.11 | -0.44 | 4.28 | 2.91 |
Nuclear Periphery | -0.79 | -6.32 | -1.95 | -1.53 | 3.99 | -3.62 | -4.75 | -1.61 | -0.15 | -0.11 | -3.40 | -6.82 | -2.49 | -1.34 | 3.71 |
Nuclear Periphery Foci | -0.33 | -4.92 | -1.70 | -1.56 | 2.55 | -3.37 | -2.83 | -1.12 | -0.74 | -0.99 | -4.09 | -6.59 | -3.13 | -0.31 | 1.66 |
Nucleolus | 2.69 | -6.84 | 0.85 | -2.82 | 7.80 | -5.85 | -6.73 | -1.51 | -0.49 | 2.76 | -6.74 | -11.67 | -6.26 | -0.20 | 9.18 |
Nucleus | 4.52 | 0.96 | 3.58 | -2.53 | 4.41 | -6.53 | -6.11 | -1.68 | -0.31 | 3.51 | -5.18 | -9.63 | -5.32 | -1.21 | 6.13 |
Peroxisomes | 0.92 | 1.73 | 1.79 | 0.53 | -0.05 | -5.47 | -5.42 | -1.07 | -0.54 | -0.72 | -5.63 | -4.09 | -3.50 | 1.51 | -0.21 |
Vacuole | 11.11 | 15.83 | -0.78 | -24.73 | -35.05 | -0.21 | -9.07 | -6.63 | -6.49 | -2.68 | 3.94 | 11.64 | -22.62 | -26.39 | -31.47 |
Vacuole Periphery | -44.92* | -61.13* | -18.58 | 27.44* | 42.32* | 19.92* | 22.42 | 19.92 | 9.28 | 3.48 | 10.48 | -5.67 | 36.50 | 26.84 | 37.35* |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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