Standard name
Human Ortholog
Description Palmitoylated vacuolar membrane-localized casein kinase I isoform; negatively regulates vacuole fusion during hypertonic stress via phosphorylation of Vps41p; shares essential functions with Hrr25p; regulates vesicle fusion in AP-3 pathway
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.15 0.11 0.15 0.23 0.26 0.27 0.22 0.24 – 0.29 0.34 0.33 0.09 0.09 0.11 0.14 0.17 0.15 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0.05 0 0 – 0 0 0 0 0 0 0.05 0.05 0 0 0 0 0 0 0
Endosome 0.18 0.07 0.49 0.37 0.15 0.07 0.09 0.08 – 0 0 0 0.18 0.16 0.18 0.09 0.13 0.11 0.22 0.21 0.3 0.26 0.27 0.26
Golgi 0 0 0 0.06 0 0 0 0 – 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Mitochondria 0 0 0 0.06 0 0.28 0.37 0.29 – 0.52 0.47 0.37 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0.05 0.05 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0.05 0 0 0 0 0 0.07 0 0.06 0.05
Nucleolus 0 0 0 0 0 0 0 0 – 0 0 0 0.06 0.06 0.05 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0.05 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.85 0.87 0.63 0.49 0.66 0.54 0.48 0.49 – 0.37 0.38 0.42 0.79 0.8 0.8 0.8 0.78 0.79 0.66 0.67 0.56 0.57 0.58 0.56
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 2 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 1 0 0 2
Bud 1 1 1 1 0 0 2 2 – 1 1 0 0 0 0 0 0 0 5 7 4 5 4 10
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 1 0 2 2
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 2 – 1 1 2 2 2 0 0 0 0 0 0 0 0 0 0
Cytoplasm 60 38 61 54 53 69 65 74 – 85 79 92 10 10 14 45 58 39 6 6 3 9 8 8
Endoplasmic Reticulum 1 5 11 9 0 14 11 8 – 12 3 3 1 1 3 15 18 9 1 0 2 2 4 5
Endosome 76 24 201 86 30 18 26 24 – 4 4 11 21 19 23 29 44 28 97 79 126 55 123 114
Golgi 0 0 4 13 6 8 1 1 – 0 0 0 1 1 2 3 4 14 3 2 2 0 0 1
Mitochondria 0 1 0 13 5 74 111 90 – 151 109 103 0 0 0 12 5 8 1 3 3 3 5 5
Nucleus 8 5 0 0 2 2 3 5 – 4 5 7 5 5 7 15 11 10 12 6 4 2 5 7
Nuclear Periphery 0 1 2 4 0 7 4 1 – 3 0 2 2 1 7 9 11 3 10 6 31 9 25 21
Nucleolus 15 9 18 7 0 1 0 3 – 1 2 0 7 7 6 5 15 8 3 2 0 0 3 6
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
SpindlePole 4 2 19 4 2 0 0 1 – 0 1 0 2 3 1 3 4 3 2 3 4 1 3 3
Vac/Vac Membrane 351 303 259 112 134 140 143 150 – 109 87 118 91 93 103 249 261 208 288 251 236 124 265 252
Unique Cell Count 413 348 414 230 203 260 298 309 291 231 281 115 116 129 313 334 264 436 375 425 219 455 449
Labelled Cell Count 516 389 578 303 232 333 366 361 371 292 338 142 142 166 385 431 330 436 375 425 219 455 449


Vacuole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 9.8 9.0 8.6 8.1 8.0 6.3 6.2 6.4 – 5.7 5.8 6.1 9.9 9.9 9.7 8.7 10.0 10.3 9.9 10.1 10.6
Std Deviation (1e-4) 1.3 1.4 1.2 1.4 1.4 1.1 1.5 1.5 – 1.2 1.1 1.4 1.3 1.3 1.6 1.7 2.2 2.3 2.0 1.6 2.1
Intensity Change (Log2) – – – -0.08 -0.1 -0.44 -0.46 -0.43 – -0.58 -0.57 -0.5 0.21 0.21 0.18 0.01 0.22 0.26 0.21 0.24 0.3


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cytoplasm 2.8 3.4 3.8 2.4 3.1 – 4.7 5.7 5.6 -1.7 -1.7 -1.1 -0.1 1.0 0
Endoplasmic Reticulum 0.9 0 1.8 0.8 -0.1 – 1.1 -1.1 -1.5 0 0 0 1.5 1.9 0.6
Endosome -2.7 -8.1 -11.2 -11.2 -11.7 – -13.6 -12.2 -12.5 -5.8 -6.2 -6.2 -11.3 -10.2 -10.2
Golgi 3.6 0 0 0 0 – 0 0 0 0 0 0 0 0 3.4
Mitochondria 0 0 11.5 13.5 11.7 – 16.5 15.3 13.3 0 0 0 4.0 0 0
Nucleus 0 0 0 0 0 – 0 0 0 0 0 0 4.5 0 0
Nuclear Periphery 0 0 0 0 0 – 0 0 0 0 0 0 0 2.9 0
Nucleolus -0.8 -3.0 -3.0 -3.6 -2.7 – -3.2 -2.4 -3.5 0.8 0.8 0.1 -2.1 0.1 -0.9
Peroxisomes 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
SpindlePole -1.9 -2.3 -3.5 -3.7 -3.5 – -3.7 -2.9 -3.6 0 0 0 -2.8 -2.7 -2.5
Vacuole -3.4 0.8 -2.2 -3.9 -3.8 – -6.6 -6.1 -5.3 3.3 3.5 3.6 4.9 4.6 4.5

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 7.0277 6.3403 5.9014 5.8189 5.8536 6.4771 8.4626 9.5571 8.8926 8.237 8.9274 8.9861 8.6093 10.1935 9.5741 9.284 8.9746 9.4157
Actin 0.0117 0.0002 0.0008 0.0001 0 0.0002 0.0257 0 0.013 0.0252 0.0037 0.0055 0 0 0 0 0 0
Bud 0.0013 0.0035 0.0021 0.0002 0.0005 0.0053 0.001 0.0004 0.0017 0.0004 0.0004 0.0004 0.0005 0.0002 0.0004 0.0002 0.0004 0.0003
Bud Neck 0.0012 0.0007 0.0025 0.0009 0.0003 0.0038 0.0012 0.0008 0.0012 0.0002 0.0005 0.0014 0.0007 0.0004 0.0029 0.0001 0.0005 0.0008
Bud Periphery 0.0021 0.0027 0.0017 0.0002 0.0004 0.0034 0.0014 0.0003 0.0016 0.0003 0.0002 0.0004 0.0007 0.0002 0.0005 0.0002 0.0002 0.0004
Bud Site 0.0014 0.0076 0.0056 0.0005 0.0007 0.0032 0.0015 0.0017 0.0048 0.0009 0.0004 0.0006 0.0032 0.0006 0.0013 0.0001 0.0011 0.0005
Cell Periphery 0.0007 0.0006 0.0005 0.0002 0.0002 0.0028 0.0005 0.0004 0.0004 0.0001 0.0001 0.0001 0.0004 0.0003 0.0003 0.0001 0.0001 0.0006
Cytoplasm 0.0248 0.0898 0.0644 0.0347 0.0203 0.0379 0.0723 0.099 0.1278 0.0722 0.0732 0.1059 0.0151 0.0292 0.0184 0.0123 0.0113 0.021
Cytoplasmic Foci 0.0196 0.0196 0.0207 0.0264 0.0132 0.0264 0.0364 0.0119 0.0305 0.0116 0.0171 0.0333 0.0096 0.0062 0.01 0.008 0.0073 0.013
Eisosomes 0.0001 0 0 0 0 0 0.0002 0 0.0001 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0.0074 0.004 0.0053 0.005 0.0011 0.0096 0.0147 0.0107 0.0086 0.0022 0.0037 0.0065 0.005 0.0098 0.0059 0.0033 0.0016 0.0041
Endosome 0.261 0.1543 0.2304 0.2141 0.2201 0.2108 0.3375 0.1808 0.289 0.344 0.283 0.2998 0.2734 0.1797 0.2375 0.298 0.2303 0.2141
Golgi 0.0055 0.0021 0.0043 0.0057 0.0023 0.0033 0.0193 0.0016 0.0144 0.0044 0.0059 0.0142 0.0033 0.0018 0.0047 0.0017 0.0023 0.0038
Lipid Particles 0.0068 0.0022 0.0039 0.0083 0.0004 0.0018 0.0166 0.0006 0.0038 0.0009 0.0154 0.0036 0.0124 0.0024 0.0017 0.0017 0.0011 0.003
Mitochondria 0.0213 0.0027 0.0038 0.0096 0.0003 0.0012 0.0238 0.0005 0.0153 0.0021 0.0008 0.0053 0.0014 0.0004 0.0018 0.0024 0.0002 0.0007
None 0.0412 0.0238 0.0561 0.0012 0.0001 0.0063 0.0029 0.0004 0.0014 0.0009 0.0007 0.0008 0.0002 0.0001 0.0001 0.0001 0.0001 0.0002
Nuclear Periphery 0.0257 0.0303 0.0151 0.0147 0.0058 0.0353 0.0194 0.0366 0.0124 0.0059 0.0072 0.0179 0.0236 0.0289 0.0349 0.0151 0.0058 0.0159
Nucleolus 0.0093 0.0034 0.0035 0.0007 0.0005 0.0013 0.0008 0.0018 0.0004 0.0003 0.0004 0.0007 0.0038 0.0085 0.0022 0.0002 0.0004 0.0018
Nucleus 0.0156 0.0267 0.017 0.0196 0.0021 0.0163 0.0255 0.0082 0.0054 0.0021 0.003 0.0099 0.0035 0.004 0.0043 0.0014 0.0025 0.0046
Peroxisomes 0.0043 0.0014 0.0014 0.0162 0.0003 0.0005 0.004 0.0001 0.0073 0.0033 0.0071 0.0018 0.0004 0.0001 0.0002 0 0.0001 0.0003
Punctate Nuclear 0.0093 0.0012 0.003 0.0007 0.0001 0.0065 0.0015 0.0002 0.0008 0.0028 0.0007 0.0018 0.0003 0.0005 0.0005 0 0.0001 0.002
Vacuole 0.4362 0.5104 0.417 0.4772 0.4871 0.4506 0.312 0.5236 0.377 0.3778 0.4109 0.3767 0.4803 0.4793 0.4253 0.4152 0.5103 0.4782
Vacuole Periphery 0.0934 0.1129 0.1408 0.164 0.2442 0.1738 0.0817 0.1205 0.0832 0.1422 0.1654 0.1132 0.1624 0.2472 0.2469 0.2399 0.2245 0.2347

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 16.1132 19.4144 15.3358 25.9629 19.529 10.5028 22.6377 20.9417 21.7513 27.86
Translational Efficiency 1.2816 1.0625 1.2713 0.8359 1.0066 1.9649 1.0097 1.064 1.0545 0.8955

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Palmitoylated vacuolar membrane-localized casein kinase I isoform; negatively regulates vacuole fusion during hypertonic stress via phosphorylation of Vps41p; shares essential functions with Hrr25p; regulates vesicle fusion in AP-3 pathway
Localization
Cell Percentages vacuolar membrane (82%)
Cell Cycle Regulation No
Subcompartmental Group vac memb-1

Yck3

Yck3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Yck3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available