Standard name | |
---|---|
Human Ortholog | |
Description | Protein that interacts with Vps4p and has a role in vacuolar sorting; stimulates the ATPase activity of Vps4; localizes to endosomes in a Vps4-dependent manner; overexpression causes canavanine sensitivity and confers a partial class D vacuole morphology |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 10 | 15 | 9 | 4 | 1 | 0 | 2 | – | 0 | 1 | 1 | 0 | 8 | 4 | 4 | 21 | 6 | 10 | 9 | 8 | 5 | 2 | 3 | 4 |
Bud | 8 | 4 | 5 | 11 | 2 | 23 | 10 | – | 15 | 7 | 13 | 21 | 4 | 3 | 2 | 2 | 1 | 2 | 2 | 0 | 0 | 0 | 2 | 4 |
Bud Neck | 2 | 1 | 2 | 3 | 2 | 3 | 3 | – | 2 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 0 | 1 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 4 | 0 | – | 2 | 1 | 3 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 3 | 1 | – | 0 | 3 | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 149 | 134 | 89 | 25 | 79 | 127 | 267 | – | 176 | 157 | 140 | 195 | 52 | 147 | 205 | 261 | 189 | 121 | 74 | 76 | 54 | 33 | 65 | 72 |
Endoplasmic Reticulum | 13 | 42 | 6 | 0 | 0 | 0 | 0 | – | 0 | 2 | 1 | 1 | 11 | 4 | 6 | 18 | 10 | 11 | 1 | 0 | 2 | 0 | 2 | 3 |
Endosome | 190 | 58 | 36 | 58 | 37 | 18 | 44 | – | 23 | 20 | 19 | 16 | 41 | 102 | 66 | 118 | 74 | 61 | 300 | 186 | 112 | 104 | 159 | 124 |
Golgi | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 2 | 2 | 3 | 2 | 1 | 2 | 1 |
Mitochondria | 6 | 9 | 1 | 12 | 4 | 82 | 27 | – | 114 | 85 | 127 | 136 | 2 | 0 | 1 | 7 | 5 | 3 | 6 | 13 | 12 | 2 | 13 | 12 |
Nucleus | 0 | 0 | 4 | 3 | 4 | 2 | 11 | – | 6 | 5 | 5 | 4 | 2 | 0 | 3 | 44 | 46 | 34 | 0 | 0 | 3 | 1 | 0 | 4 |
Nuclear Periphery | 1 | 1 | 0 | 1 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 1 | 3 | 1 | – | 0 | 0 | 1 | 2 | 0 | 0 | 0 | 4 | 3 | 1 | 0 | 0 | 0 | 0 | 0 | 1 |
Peroxisomes | 1 | 0 | 0 | 12 | 6 | 2 | 10 | – | 4 | 1 | 1 | 6 | 1 | 4 | 0 | 2 | 0 | 0 | 2 | 0 | 1 | 0 | 0 | 0 |
SpindlePole | 46 | 18 | 40 | 59 | 65 | 37 | 84 | – | 25 | 35 | 24 | 30 | 14 | 17 | 12 | 50 | 24 | 14 | 29 | 22 | 20 | 10 | 16 | 6 |
Vac/Vac Membrane | 26 | 9 | 2 | 9 | 12 | 3 | 23 | – | 18 | 7 | 5 | 7 | 4 | 6 | 3 | 32 | 14 | 13 | 10 | 8 | 1 | 5 | 7 | 5 |
Unique Cell Count | 376 | 251 | 170 | 145 | 187 | 246 | 408 | 302 | 283 | 281 | 335 | 110 | 259 | 283 | 477 | 323 | 238 | 448 | 328 | 222 | 167 | 283 | 248 | |
Labelled Cell Count | 453 | 291 | 194 | 197 | 213 | 307 | 483 | 385 | 325 | 341 | 421 | 139 | 288 | 303 | 561 | 373 | 274 | 448 | 328 | 222 | 167 | 283 | 248 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.6 | 5.6 | 6.5 | 5.6 | 6.2 | 4.3 | 5.3 | – | 4.3 | 4.4 | 4.3 | 4.4 | 5.9 | 6.7 | 6.6 | 8.4 | 8.9 | 8.1 | 5.4 | 5.9 | 6.2 |
Std Deviation (1e-4) | 0.7 | 0.8 | 2.4 | 1.1 | 1.5 | 0.9 | 4.8 | – | 1.0 | 1.1 | 0.8 | 1.1 | 1.0 | 1.5 | 1.4 | 1.4 | 1.4 | 1.4 | 1.0 | 1.6 | 1.8 |
Intensity Change (Log2) | – | – | – | -0.2 | -0.08 | -0.61 | -0.29 | – | -0.6 | -0.56 | -0.6 | -0.58 | -0.15 | 0.04 | 0.01 | 0.38 | 0.45 | 0.31 | -0.28 | -0.14 | -0.06 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | -1.1 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0.7 | -2.2 | 0 | -0.5 | -2.1 | -0.5 |
Bud | 1.9 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -6.5 | -1.9 | -0.1 | 2.9 | – | 1.2 | 0.6 | -0.5 | 1.3 | -0.8 | 0.9 | 4.3 | 0.5 | 1.3 | -0.3 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 2.2 | 0 | 0 | 0.1 | -0.3 | 0.5 |
Endosome | 3.6 | -0.3 | 0 | -3.3 | – | 0 | 0 | 0 | 0 | 2.9 | 3.9 | 0.5 | 0.9 | 0.4 | 1.0 |
Golgi | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 3.4 | 0 | 0 | 3.1 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.9 | 4.2 | 4.1 |
Nuclear Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 3.8 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 3.3 | 2.3 | 0 | -0.8 | – | 0 | 0 | 0 | 0 | -2.2 | -5.1 | -6.2 | -4.2 | -5.1 | -5.2 |
Vacuole | 2.4 | 2.5 | 0 | 2.4 | – | 2.5 | 0 | 0 | 0 | 0 | 0 | 0 | 2.8 | 1.9 | 2.3 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | -0.3074 | -0.2478 | -0.2748 | -0.2861 | -0.7219 | 0.0185 | 1.3275 | 0.888 | 1.1323 | 0.9122 | 0.6842 | 0.9565 | 2.6991 | 3.836 | 3.157 | 3.0261 | 2.7397 | 3.4353 |
Actin | 0.0234 | 0.0001 | 0.0073 | 0.0572 | 0.004 | 0.0026 | 0.0161 | 0.0004 | 0.0096 | 0.0097 | 0.0145 | 0.0031 | 0.0003 | 0.001 | 0.0002 | 0.0004 | 0.0001 | 0.0002 |
Bud | 0.0004 | 0.0001 | 0.0002 | 0.0007 | 0.0002 | 0.0003 | 0.0003 | 0.0002 | 0.0004 | 0.0002 | 0.0004 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0.0002 | 0 | 0.0001 |
Bud Neck | 0.0015 | 0.0016 | 0.0013 | 0.0111 | 0.0026 | 0.0059 | 0.0011 | 0.001 | 0.0026 | 0.0012 | 0.0018 | 0.0032 | 0.0009 | 0.001 | 0.0015 | 0.0015 | 0.0005 | 0.004 |
Bud Periphery | 0.0005 | 0.0001 | 0.0011 | 0.0012 | 0.0003 | 0.0007 | 0.0006 | 0.0002 | 0.0006 | 0.0007 | 0.0006 | 0.0005 | 0.0002 | 0.0002 | 0.0002 | 0.0002 | 0 | 0.0001 |
Bud Site | 0.0021 | 0.0021 | 0.0016 | 0.0052 | 0.0015 | 0.0007 | 0.0011 | 0.002 | 0.0062 | 0.0005 | 0.0054 | 0.0003 | 0.0006 | 0.0015 | 0.0012 | 0.0007 | 0.0001 | 0.0004 |
Cell Periphery | 0.0002 | 0.0002 | 0.0016 | 0.0008 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0 | 0.0001 |
Cytoplasm | 0.0385 | 0.0437 | 0.0298 | 0.0337 | 0.0142 | 0.0303 | 0.0229 | 0.0354 | 0.0212 | 0.0297 | 0.0179 | 0.0117 | 0.0327 | 0.0483 | 0.0239 | 0.0236 | 0.0108 | 0.0224 |
Cytoplasmic Foci | 0.0375 | 0.0051 | 0.0054 | 0.008 | 0.0089 | 0.0017 | 0.01 | 0.0013 | 0.0054 | 0.0044 | 0.0147 | 0.0024 | 0.0015 | 0.0029 | 0.0019 | 0.0014 | 0.0004 | 0.0018 |
Eisosomes | 0.0002 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0 | 0.0001 | 0.0002 | 0.0002 | 0 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 |
Endoplasmic Reticulum | 0.0036 | 0.0035 | 0.0057 | 0.0075 | 0.0024 | 0.0007 | 0.0052 | 0.002 | 0.0024 | 0.0022 | 0.0024 | 0.0011 | 0.0019 | 0.0027 | 0.0021 | 0.0012 | 0.0006 | 0.0011 |
Endosome | 0.0295 | 0.0094 | 0.0138 | 0.0166 | 0.0415 | 0.0033 | 0.0254 | 0.0026 | 0.0136 | 0.0167 | 0.0213 | 0.0044 | 0.004 | 0.0039 | 0.0042 | 0.0014 | 0.0006 | 0.0027 |
Golgi | 0.0073 | 0.0002 | 0.0023 | 0.0014 | 0.0172 | 0.0007 | 0.0039 | 0.0001 | 0.0079 | 0.009 | 0.0129 | 0.0023 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0 | 0.0001 |
Lipid Particles | 0.0129 | 0.0028 | 0.0061 | 0.0061 | 0.0322 | 0.0044 | 0.0068 | 0.0004 | 0.0047 | 0.0421 | 0.0039 | 0.0058 | 0.0004 | 0.0013 | 0.0013 | 0.0002 | 0.0001 | 0.0009 |
Mitochondria | 0.0135 | 0.0005 | 0.0086 | 0.0034 | 0.0133 | 0.0022 | 0.0032 | 0.0006 | 0.005 | 0.0211 | 0.0019 | 0.006 | 0.0008 | 0.0008 | 0.0006 | 0.0009 | 0.0009 | 0.0003 |
None | 0.0776 | 0.0577 | 0.0919 | 0.1451 | 0.0376 | 0.0021 | 0.0503 | 0.0432 | 0.0799 | 0.0607 | 0.0667 | 0.0308 | 0.033 | 0.1132 | 0.0762 | 0.0556 | 0.0384 | 0.0569 |
Nuclear Periphery | 0.0475 | 0.0343 | 0.0409 | 0.0563 | 0.0129 | 0.0092 | 0.0439 | 0.0235 | 0.0275 | 0.0216 | 0.0233 | 0.0164 | 0.014 | 0.0207 | 0.012 | 0.0139 | 0.0138 | 0.0075 |
Nucleolus | 0.0353 | 0.0867 | 0.0563 | 0.1518 | 0.0816 | 0.0754 | 0.0566 | 0.0552 | 0.0685 | 0.0963 | 0.0268 | 0.0803 | 0.056 | 0.0744 | 0.1129 | 0.0477 | 0.0191 | 0.0879 |
Nucleus | 0.6014 | 0.6958 | 0.6994 | 0.4557 | 0.6268 | 0.8181 | 0.7036 | 0.7918 | 0.6874 | 0.5485 | 0.7125 | 0.7538 | 0.8004 | 0.6797 | 0.7141 | 0.8262 | 0.8656 | 0.7463 |
Peroxisomes | 0.0046 | 0.0001 | 0.0019 | 0.0017 | 0.0035 | 0.0029 | 0.0078 | 0.0001 | 0.0022 | 0.019 | 0.0038 | 0.0025 | 0.0001 | 0.0004 | 0.0001 | 0.0002 | 0 | 0.0001 |
Punctate Nuclear | 0.0569 | 0.0449 | 0.0162 | 0.0158 | 0.0931 | 0.0357 | 0.036 | 0.0367 | 0.0487 | 0.1119 | 0.066 | 0.0734 | 0.0472 | 0.0391 | 0.0419 | 0.0228 | 0.0478 | 0.0655 |
Vacuole | 0.0042 | 0.0098 | 0.0062 | 0.0167 | 0.0041 | 0.0025 | 0.0029 | 0.0027 | 0.0044 | 0.0023 | 0.0022 | 0.0012 | 0.0052 | 0.0071 | 0.0047 | 0.0015 | 0.0005 | 0.0013 |
Vacuole Periphery | 0.0014 | 0.0012 | 0.0021 | 0.004 | 0.0017 | 0.0005 | 0.0021 | 0.0004 | 0.0015 | 0.002 | 0.0006 | 0.0006 | 0.0006 | 0.0011 | 0.0004 | 0.0003 | 0.0004 | 0.0002 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 7.2118 | 6.4469 | 4.7839 | 6.4792 | 7.0128 | 12.6137 | 6.1111 | 7.1492 | 5.4542 | 9.1061 |
Translational Efficiency | 2.4892 | 1.607 | 2.1534 | 1.3186 | 2.1154 | 1.0506 | 1.3944 | 1.6931 | 2.1401 | 1.7585 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
893 | 1404 | 103 | 1553 | 488 | 1829 | 1854 | 1104 | 1381 | 3233 | 1957 | 2657 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 627.36 | 741.84 | 896.12 | 760.24 | 702.90 | 770.64 | 835.04 | 863.28 | 654.05 | 758.13 | 838.25 | 803.05 |
Standard Deviation | 67.55 | 115.96 | 90.51 | 105.45 | 78.51 | 95.57 | 97.90 | 120.10 | 80.20 | 105.88 | 98.48 | 122.77 |
Intensity Change Log 2 | — | 0.241815 | 0.514398 | 0.277161 | — | 0.132738 | 0.248526 | 0.296509 | — | 0.185208 | 0.380031 | 0.287417 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000064 | 0.000338 | 0.000590 | 0.000503 | 0.000250 | 0.000162 | 0.000242 | 0.000476 | 0.000130 | 0.000239 | 0.000260 | 0.000492 |
Bud Neck | 0.004348 | 0.009459 | 0.002341 | 0.008456 | 0.001165 | 0.009610 | 0.005593 | 0.016016 | 0.003223 | 0.009544 | 0.005422 | 0.011597 |
Bud Site | 0.000836 | 0.004982 | 0.000609 | 0.002946 | 0.000440 | 0.004917 | 0.001017 | 0.015992 | 0.000697 | 0.004946 | 0.000995 | 0.008367 |
Cell Periphery | 0.000214 | 0.000168 | 0.000072 | 0.000116 | 0.000105 | 0.000126 | 0.000084 | 0.000204 | 0.000176 | 0.000144 | 0.000083 | 0.000153 |
Cytoplasm | 0.117733* | 0.155417* | 0.013478 | 0.073651 | 0.088313 | 0.096512 | 0.029302 | 0.101712* | 0.107337* | 0.122093* | 0.028469 | 0.085310 |
Cytoplasmic Foci | 0.055752 | 0.021213 | 0.000703 | 0.001734 | 0.027033 | 0.018420 | 0.000638 | 0.002883 | 0.045604 | 0.019633 | 0.000641 | 0.002211 |
Eisosomes | 0.000069 | 0.000037 | 0.000052 | 0.000025 | 0.000052 | 0.000032 | 0.000019 | 0.000032 | 0.000063 | 0.000034 | 0.000020 | 0.000028 |
Endoplasmic Reticulum | 0.001455 | 0.004992 | 0.008979 | 0.006708 | 0.002535 | 0.002459 | 0.004292 | 0.006392 | 0.001836 | 0.003559 | 0.004539 | 0.006576 |
Endosome | 0.001515 | 0.003361 | 0.000741 | 0.002154 | 0.001397 | 0.002807 | 0.000426 | 0.003788 | 0.001473 | 0.003047 | 0.000442 | 0.002833 |
Golgi | 0.000974 | 0.000553 | 0.000064 | 0.000521 | 0.000446 | 0.000937 | 0.000041 | 0.002047 | 0.000788 | 0.000771 | 0.000042 | 0.001155 |
Lipid Particles | 0.015578 | 0.003229 | 0.001168 | 0.000681 | 0.006053 | 0.003097 | 0.000368 | 0.000671 | 0.012212 | 0.003155 | 0.000410 | 0.000677 |
Mitochondria | 0.006579 | 0.006521 | 0.000554 | 0.001569 | 0.005574 | 0.006915 | 0.000787 | 0.002632 | 0.006224 | 0.006744 | 0.000775 | 0.002011 |
Mitotic Spindle | 0.000226 | 0.004634 | 0.000054 | 0.009790 | 0.000426 | 0.006088 | 0.000906 | 0.030481 | 0.000297 | 0.005457 | 0.000861 | 0.018387 |
None | 0.016793 | 0.021627 | 0.003261 | 0.007522 | 0.008604 | 0.008435 | 0.005452 | 0.008483 | 0.013899 | 0.014164 | 0.005337 | 0.007921 |
Nuclear Periphery | 0.002051 | 0.002310 | 0.001560 | 0.002914 | 0.003436 | 0.002417 | 0.001494 | 0.003035 | 0.002540 | 0.002370 | 0.001498 | 0.002964 |
Nuclear Periphery Foci | 0.000621 | 0.001315 | 0.001479 | 0.001024 | 0.001178 | 0.000559 | 0.000236 | 0.000693 | 0.000818 | 0.000887 | 0.000302 | 0.000887 |
Nucleolus | 0.014441 | 0.012173 | 0.007960 | 0.005729 | 0.008816 | 0.010230 | 0.003616 | 0.003062 | 0.012454 | 0.011074 | 0.003845 | 0.004621 |
Nucleus | 0.751825* | 0.724574* | 0.948083* | 0.860905* | 0.836337* | 0.816007* | 0.941013* | 0.770765* | 0.781689* | 0.776300* | 0.941385* | 0.823451* |
Peroxisomes | 0.004943 | 0.002766 | 0.000358 | 0.000279 | 0.002575 | 0.002629 | 0.000175 | 0.000513 | 0.004106 | 0.002689 | 0.000185 | 0.000377 |
Vacuole | 0.003560 | 0.018800 | 0.007750 | 0.011814 | 0.004838 | 0.006756 | 0.004181 | 0.026275 | 0.004012 | 0.011986 | 0.004369 | 0.017823 |
Vacuole Periphery | 0.000421 | 0.001529 | 0.000144 | 0.000957 | 0.000427 | 0.000887 | 0.000119 | 0.003849 | 0.000423 | 0.001166 | 0.000120 | 0.002159 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -8.39 | -5.30 | -6.74 | -2.59 | 1.65 | 0.79 | -0.36 | -2.47 | -5.24 | -3.65 | -2.24 | -4.07 | -6.36 | -5.89 | -4.31 |
Bud Neck | -3.79 | 0.69 | -5.09 | -0.79 | -7.87 | -8.67 | -11.00 | -12.25 | -5.50 | -6.78 | -6.72 | -5.49 | -11.06 | -4.52 | -6.37 |
Bud Site | -4.19 | -0.87 | -4.30 | 1.55 | -3.16 | -5.88 | -2.79 | -6.60 | -3.61 | -6.08 | -6.88 | -1.74 | -7.72 | -1.83 | -6.88 |
Cell Periphery | 1.49 | 3.56 | 3.62 | 3.56 | -0.77 | -1.13 | 1.71 | -1.97 | -1.31 | -3.10 | 1.65 | 4.54 | 2.22 | 1.01 | -3.36 |
Cytoplasm | -4.56 | 13.30 | 6.55 | 11.75 | -9.09 | -1.05 | 8.35 | -0.40 | 0.80 | -12.24 | -2.68 | 16.39 | 4.97 | 9.17 | -15.05 |
Cytoplasmic Foci | 7.89 | 13.88 | 13.67 | 10.42 | -1.93 | 2.85 | 8.79 | 8.23 | 11.10 | -4.99 | 8.72 | 16.23 | 15.69 | 15.26 | -5.67 |
Eisosomes | 7.29 | -0.05 | 8.75 | 1.53 | 3.00 | 4.02 | 5.69 | 1.64 | -4.99 | -8.79 | 8.90 | 11.75 | 7.51 | -2.19 | -7.13 |
Endoplasmic Reticulum | -10.62 | -6.93 | -19.65 | -7.05 | 1.86 | 0.29 | -7.29 | -12.29 | -14.32 | -7.69 | -8.14 | -14.43 | -22.95 | -16.15 | -8.48 |
Endosome | -3.89 | 1.07 | -0.88 | 2.91 | -1.87 | -2.45 | 3.63 | -2.17 | 0.11 | -4.44 | -4.15 | 4.69 | -2.08 | 1.93 | -5.97 |
Golgi | 1.08 | 2.64 | 1.63 | 1.23 | -1.53 | -1.40 | 2.08 | -0.69 | -0.02 | -1.31 | 0.02 | 3.27 | 0.51 | 0.54 | -1.84 |
Lipid Particles | 6.80 | 7.81 | 8.33 | 7.81 | 2.02 | 2.67 | 5.08 | 4.67 | 7.02 | -6.70 | 7.32 | 9.68 | 9.37 | 10.38 | -5.89 |
Mitochondria | 0.09 | 5.19 | 4.34 | 4.31 | -5.13 | -0.57 | 2.21 | 1.58 | 4.86 | -5.24 | -0.35 | 5.10 | 4.16 | 6.65 | -6.17 |
Mitotic Spindle | -3.23 | 0.16 | -5.13 | -2.09 | -5.14 | -4.30 | -2.58 | -6.51 | -4.48 | -5.81 | -5.42 | -2.78 | -8.27 | -4.65 | -6.71 |
None | -1.56 | 5.92 | 4.78 | 7.20 | -2.44 | 0.07 | 1.77 | 0.87 | 1.30 | -1.40 | -0.02 | 5.58 | 4.61 | 6.47 | -1.82 |
Nuclear Periphery | -0.95 | 0.33 | -5.55 | -4.98 | -4.33 | 0.72 | 1.00 | -0.86 | -6.18 | -10.01 | 0.25 | 0.93 | -3.17 | -7.48 | -11.23 |
Nuclear Periphery Foci | -2.65 | -1.72 | -2.72 | -0.87 | 0.29 | 1.93 | 2.47 | 0.70 | -3.24 | -4.95 | -0.16 | 2.62 | -2.05 | -2.06 | -4.02 |
Nucleolus | 1.47 | 1.18 | 6.25 | 5.41 | 1.37 | -0.65 | 2.51 | 3.20 | 5.72 | 1.69 | 1.26 | 6.81 | 7.16 | 7.31 | 0.33 |
Nucleus | 2.01 | -13.08 | -8.94 | -12.37 | 7.27 | 1.43 | -9.23 | 5.35 | 5.26 | 19.45 | 0.31 | -19.98 | -3.81 | -5.13 | 21.66 |
Peroxisomes | 2.85 | 7.53 | 7.90 | 5.44 | 1.57 | 0.25 | 3.95 | 3.70 | 7.01 | -1.91 | 2.97 | 8.85 | 8.67 | 8.73 | -1.62 |
Vacuole | -6.91 | -2.61 | -10.58 | -1.77 | -2.66 | -1.67 | -1.67 | -13.37 | -12.98 | -12.96 | -7.22 | -3.82 | -16.89 | -11.60 | -14.74 |
Vacuole Periphery | -4.21 | 2.39 | -1.24 | 2.53 | -2.49 | -1.65 | 2.48 | -2.06 | -1.36 | -2.57 | -3.92 | 3.80 | -2.40 | -0.07 | -3.45 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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