ORF
Human Ortholog
Description Magnesium-dependent acid phosphatase; member of the haloacid dehalogenase superfamily; non-essential gene
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.99 0.99 0.97 0.95 0.83 0.85 0.75 – 0.73 0.67 0.74 0.66 0.99 1.0 0.99 0.96 0.97 0.99 0.96 0.96 0.93 0.93 0.97 0.95
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.14 0 – 0.15 0.1 0.16 0.18 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 – 0 0 0.08 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0.08 0.24 0.1 0.27 – 0.15 0.27 0.14 0.18 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 1 0 – 2 0 0 2 0 0 2 0 0 0 0 0 0 0 1 1
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 2 0 0 0 3 2 2 – 5 9 2 2 0 3 3 8 9 5 0 0 0 0 0 0
Cytoplasm 346 170 87 113 151 112 193 – 159 180 104 94 213 354 377 205 196 172 333 165 90 139 436 310
Endoplasmic Reticulum 0 1 0 0 1 0 0 – 2 1 0 3 0 0 0 6 4 1 1 0 1 0 1 1
Endosome 0 0 0 0 0 0 1 – 1 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0
Golgi 1 0 0 0 0 0 0 – 0 0 0 0 1 0 0 1 1 0 2 1 0 0 0 0
Mitochondria 1 0 0 1 0 19 7 – 33 28 23 26 0 0 0 2 0 0 1 0 0 1 0 2
Nucleus 3 1 2 2 1 5 8 – 7 11 11 7 4 1 3 4 3 0 0 1 1 0 0 2
Nuclear Periphery 0 0 0 1 0 0 0 – 2 2 0 2 0 0 0 0 0 0 1 0 0 0 1 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 3 0 2 10 44 13 69 – 33 73 19 26 0 0 0 8 6 5 1 1 0 0 2 3
Unique Cell Count 348 171 90 119 182 132 258 218 268 140 143 215 354 380 213 203 174 347 172 98 149 450 328
Labelled Cell Count 356 172 91 127 200 152 280 244 304 159 162 218 358 385 234 219 183 347 172 98 149 450 328


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 11.6 11.8 10.1 10.2 11.1 6.8 8.9 – 8.0 8.6 7.8 7.7 12.3 12.4 13.9 13.9 13.9 13.7 10.8 12.2 13.2
Std Deviation (1e-4) 1.8 1.7 2.7 2.0 2.3 1.5 1.6 – 1.6 1.6 1.8 1.4 2.0 2.1 2.1 2.6 2.3 2.4 2.1 1.9 2.4
Intensity Change (Log2) – – – 0.02 0.14 -0.57 -0.18 – -0.32 -0.22 -0.37 -0.39 0.29 0.3 0.47 0.47 0.47 0.44 0.11 0.28 0.39


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cytoplasm -0.6 -3.2 -2.8 -4.5 – -4.7 -5.6 -4.4 -5.5 1.5 3.4 1.9 -0.2 0 1.2
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 3.8 0 – 3.9 3.2 4.1 4.3 0 0 0 0 0 0
Nucleus 0 0 0 0 – 0 0 1.8 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole 1.9 4.5 2.2 5.0 – 3.2 5.0 2.9 3.6 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.8796 5.3437 4.5104 4.0236 3.9299 4.3846 6.455 7.3792 7.4221 6.5957 7.2103 7.0655 7.7487 9.3098 8.564 8.0483 7.4829 8.4203
Actin 0.0246 0.0005 0.0011 0.0029 0.0064 0.0018 0.0263 0.0005 0.0041 0.0003 0.0239 0.007 0.0292 0.0009 0.0038 0.0025 0.0049 0.0112
Bud 0.0005 0.0001 0.0001 0.0002 0.0001 0.0001 0.0005 0.0032 0.001 0.0001 0.0001 0.0012 0.0021 0.0057 0.0031 0.0001 0.0015 0.0036
Bud Neck 0.0018 0.0001 0.0004 0.0039 0.0009 0.0009 0.0014 0.0002 0.0013 0.0006 0.0007 0.0019 0.0012 0.0003 0.0014 0.0003 0.0006 0.0012
Bud Periphery 0.0006 0 0 0.0001 0.0001 0.0001 0.0007 0.0011 0.0012 0 0.0001 0.0017 0.0013 0.0014 0.0018 0.0001 0.0031 0.0017
Bud Site 0.0011 0.0002 0.0008 0.0004 0.0002 0.0001 0.0019 0.0047 0.0045 0.0001 0.0002 0.0005 0.0028 0.0069 0.0138 0.0003 0.0007 0.0007
Cell Periphery 0.0001 0 0 0 0 0 0.0001 0 0.0001 0 0 0.0001 0.0001 0.0001 0.0003 0.0001 0.0001 0.0001
Cytoplasm 0.8746 0.9728 0.9567 0.8546 0.8619 0.9359 0.8673 0.9718 0.9281 0.9697 0.9041 0.9425 0.8488 0.961 0.9075 0.9296 0.9219 0.935
Cytoplasmic Foci 0.0119 0.0037 0.0064 0.0297 0.0201 0.0088 0.015 0.002 0.0126 0.0023 0.0083 0.0044 0.0266 0.0022 0.0088 0.0021 0.007 0.0062
Eisosomes 0.0001 0 0 0 0 0.0001 0.0001 0 0 0 0.0001 0.0001 0.0002 0 0 0.0001 0 0.0003
Endoplasmic Reticulum 0.0058 0.0029 0.0025 0.0038 0.0052 0.0027 0.0153 0.0023 0.0022 0.001 0.0074 0.0029 0.0065 0.0015 0.0011 0.0006 0.0022 0.0012
Endosome 0.0184 0.0021 0.0046 0.0204 0.0172 0.0074 0.0208 0.0023 0.0072 0.0009 0.0123 0.0063 0.0144 0.0005 0.0104 0.0009 0.0105 0.002
Golgi 0.0032 0.0001 0.0004 0.0046 0.0028 0.0016 0.0032 0.0001 0.002 0 0.0021 0.0011 0.0051 0.0001 0.0041 0.0008 0.0116 0.0016
Lipid Particles 0.0021 0 0.0002 0.0024 0.004 0.0039 0.0018 0 0.0008 0 0.0007 0.0007 0.0046 0 0.0015 0.0013 0.0013 0.0014
Mitochondria 0.003 0.0001 0.0002 0.002 0.0013 0.0008 0.002 0.0001 0.0029 0.0001 0.0007 0.0014 0.018 0.0002 0.0028 0.0494 0.0232 0.0042
None 0.0301 0.0089 0.0137 0.0065 0.0205 0.0103 0.0098 0.0037 0.005 0.0066 0.0091 0.0041 0.0165 0.0119 0.0215 0.0071 0.0018 0.018
Nuclear Periphery 0.0053 0.0017 0.0018 0.0226 0.0184 0.0044 0.0096 0.0011 0.0034 0.0015 0.004 0.0046 0.0021 0.0007 0.0007 0.0003 0.0008 0.0008
Nucleolus 0.0006 0 0.0001 0.0003 0.0002 0.0006 0.0002 0 0.0002 0 0 0.0004 0.0002 0.0001 0.0001 0.0001 0.0001 0.0002
Nucleus 0.0076 0.005 0.0083 0.0269 0.0082 0.0107 0.0119 0.0055 0.0117 0.0156 0.0222 0.015 0.0066 0.0042 0.0042 0.0024 0.0045 0.004
Peroxisomes 0.0024 0.0001 0.0002 0.0097 0.0239 0.0039 0.0071 0.0001 0.009 0 0.0004 0.0004 0.0071 0.0001 0.0111 0.0009 0.0024 0.0042
Punctate Nuclear 0.0041 0.0005 0.0014 0.0071 0.0064 0.0049 0.0024 0.0005 0.0014 0.0007 0.002 0.0019 0.0054 0.0018 0.0011 0.0001 0.0003 0.002
Vacuole 0.0017 0.0009 0.0009 0.0012 0.0014 0.0006 0.002 0.0005 0.0011 0.0004 0.0011 0.0012 0.0008 0.0003 0.0007 0.0004 0.0009 0.0003
Vacuole Periphery 0.0006 0.0001 0.0001 0.0007 0.0009 0.0003 0.0008 0.0001 0.0003 0.0001 0.0004 0.0005 0.0003 0.0001 0.0002 0.0002 0.0005 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 25.6845 27.5524 27.2601 26.2135 26.6408 24.6434 33.8942 34.4711 36.9687 28.7304
Translational Efficiency 1.2768 1.0113 1.0976 1.3398 1.555 1.6034 1.268 1.521 1.2833 1.5561

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2124 1455 3138 23 1775 2488 2327 1806 3899 3943 5465 1829

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 966.20 1062.14 1300.98 961.98 974.86 1120.10 1283.48 1154.49 970.14 1098.71 1293.53 1152.07
Standard Deviation 140.27 170.28 177.26 158.38 156.36 154.02 189.49 168.94 147.88 162.64 182.77 170.17
Intensity Change Log 2 — 0.136580 0.429205 -0.006315 — 0.200361 0.396794 0.243989 — 0.168965 0.413018 0.124815

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000101 0.000287 0.000101 0.000078 0.000139 0.000202 0.000132 0.000662 0.000118 0.000233 0.000114 0.000654
Bud Neck 0.001407 0.000926 0.001585 0.002675 0.000420 0.001818 0.001298 0.002556 0.000957 0.001489 0.001463 0.002558
Bud Site 0.003864 0.005696 0.003648 0.001749 0.003286 0.007471 0.003836 0.016109 0.003601 0.006816 0.003728 0.015929
Cell Periphery 0.000042 0.000058 0.000028 0.000280 0.000054 0.000041 0.000037 0.000101 0.000048 0.000047 0.000032 0.000103
Cytoplasm 0.796655* 0.860184* 0.713692* 0.448215* 0.860837* 0.832543* 0.675923* 0.704708* 0.825874* 0.842743* 0.697610* 0.701482*
Cytoplasmic Foci 0.054051 0.032616 0.002776 0.051738 0.020566 0.038745 0.002695 0.017192 0.038807 0.036484 0.002741 0.017627
Eisosomes 0.000010 0.000007 0.000002 0.000164 0.000004 0.000008 0.000003 0.000013 0.000007 0.000008 0.000003 0.000015
Endoplasmic Reticulum 0.000735 0.000540 0.000171 0.001157 0.000632 0.000526 0.000289 0.002319 0.000688 0.000532 0.000221 0.002305
Endosome 0.001516 0.001399 0.000105 0.007607 0.000702 0.002195 0.000397 0.005548 0.001145 0.001901 0.000230 0.005574
Golgi 0.000902 0.000793 0.000041 0.000134 0.000381 0.001100 0.000133 0.004332 0.000665 0.000987 0.000080 0.004279
Lipid Particles 0.000497 0.000365 0.000146 0.005026 0.000167 0.000348 0.000203 0.000476 0.000347 0.000354 0.000170 0.000533
Mitochondria 0.001222 0.000341 0.000306 0.000270 0.000273 0.001410 0.000384 0.002339 0.000790 0.001016 0.000339 0.002313
Mitotic Spindle 0.002469 0.002471 0.003086 0.000070 0.000945 0.010414 0.006331 0.033937 0.001776 0.007483 0.004468 0.033511
None 0.003637 0.006811 0.007970 0.001354 0.007579 0.005031 0.011621 0.007499 0.005432 0.005688 0.009524 0.007421
Nuclear Periphery 0.000193 0.000060 0.000278 0.000472 0.000055 0.000187 0.000365 0.000615 0.000130 0.000140 0.000315 0.000613
Nuclear Periphery Foci 0.001338 0.002237 0.000321 0.002686 0.000921 0.000875 0.000831 0.002027 0.001148 0.001378 0.000538 0.002035
Nucleolus 0.000234 0.000128 0.000083 0.005999 0.000191 0.000249 0.000101 0.000240 0.000214 0.000204 0.000091 0.000312
Nucleus 0.092400 0.062767 0.253589* 0.165164* 0.090511 0.060257 0.272464* 0.081028 0.091540 0.061183 0.261626* 0.082086
Peroxisomes 0.000282 0.000399 0.000081 0.001256 0.000098 0.000833 0.000200 0.001286 0.000198 0.000673 0.000132 0.001285
Vacuole 0.037830 0.021583 0.011948 0.303711* 0.011526 0.035159 0.022649 0.110492 0.025855 0.030149 0.016504 0.112922
Vacuole Periphery 0.000617 0.000332 0.000041 0.000194 0.000713 0.000586 0.000108 0.006522 0.000661 0.000492 0.000069 0.006443

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.59 -1.40 -1.40 1.09 -0.06 -1.72 -2.66 -2.55 -1.46 -1.25 -2.30 -2.49 -2.90 -0.79 -1.79
Bud Neck 1.90 -2.19 -1.08 -1.76 -0.16 -5.63 -6.26 -10.45 -0.62 -1.24 -2.82 -5.18 -6.43 -2.25 -0.66
Bud Site -1.78 0.40 0.02 1.43 -0.26 -4.54 0.09 -5.16 -0.88 -5.01 -4.46 0.37 -4.87 -1.49 -5.07
Cell Periphery -2.50 1.93 -2.95 -2.57 -3.08 3.69 0.42 -0.93 -3.10 -1.24 0.13 1.86 -2.24 -2.26 -3.03
Cytoplasm -10.85 24.85 6.56 7.58 4.36 7.07 48.95 36.61 30.64 -2.07 -2.82 49.77 33.46 34.84 4.56
Cytoplasmic Foci 7.83 25.10 1.99 -0.37 -3.90 -9.39 15.67 6.21 15.32 -15.59 1.28 28.65 18.75 17.80 -15.98
Eisosomes 2.18 9.59 -4.45 -4.54 -4.68 -8.96 -7.87 -20.39 -11.29 -15.18 -1.85 4.58 -13.94 -11.75 -18.00
Endoplasmic Reticulum 1.12 3.94 -0.81 -1.32 -2.20 0.77 2.51 -0.53 -0.81 -0.96 1.29 4.73 -0.41 -0.82 -1.28
Endosome 0.48 11.98 -2.49 -2.55 -3.38 -4.64 2.71 -3.51 0.51 -4.67 -3.17 10.77 -2.41 -0.25 -5.30
Golgi 0.51 4.84 4.37 2.49 -2.45 -2.19 3.14 -1.49 -0.06 -2.18 -1.14 5.71 -0.84 -0.25 -2.25
Lipid Particles 1.39 3.61 -1.55 -1.62 -1.67 -4.47 -12.27 -15.58 -6.93 -4.37 -0.27 -1.27 -7.54 -6.92 -8.43
Mitochondria 1.39 1.40 1.68 0.73 1.93 -2.01 -1.09 -1.45 1.07 -1.18 -0.37 1.28 0.22 0.62 -1.19
Mitotic Spindle -0.28 -1.52 0.85 1.00 2.18 -5.70 -5.53 -6.46 -1.29 -3.13 -4.87 -4.74 -5.93 -2.66 -4.15
None -3.33 -14.08 3.86 5.18 11.91 3.39 -3.85 2.92 -0.43 10.17 0.01 -8.93 0.69 0.66 7.99
Nuclear Periphery 2.53 -5.89 -3.09 -4.66 -0.47 -1.78 -28.69 -16.16 -6.27 0.96 -0.30 -16.24 -12.28 -9.28 -2.75
Nuclear Periphery Foci -3.67 4.72 -1.17 -0.26 -1.98 0.40 -2.89 -5.10 -3.32 -2.65 -1.45 3.30 -2.82 -0.87 -7.17
Nucleolus 3.49 2.62 -0.97 -1.01 -1.01 -0.82 0.58 -0.37 0.78 -2.80 0.09 1.98 -0.72 -0.79 -2.47
Nucleus 10.16 -48.99 -1.02 -1.94 5.04 9.41 -46.04 -5.36 -16.82 42.98* 14.25 -66.98 -6.30 -17.55 52.04
Peroxisomes -1.13 3.35 -0.83 -0.63 -1.17 -5.60 -0.72 -2.02 2.38 -1.65 -5.18 2.22 -1.28 1.78 -1.84
Vacuole 6.15 4.64 -5.86 -6.10 -6.02 -14.97 -19.94 -37.35 -31.08 -24.68 -5.51 -11.51 -35.05 -33.23 -31.10
Vacuole Periphery 1.85 4.73 3.75 1.56 -2.85 0.50 2.48 -1.38 -1.71 -2.68 1.01 4.45 -1.57 -1.91 -2.70
ORF
Human Ortholog
Description Magnesium-dependent acid phosphatase; member of the haloacid dehalogenase superfamily; non-essential gene
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

YER134C

YER134C


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Yer134c-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available