Standard name
Human Ortholog
Description DNA damage-inducible v-SNARE binding protein; role in suppression of protein secretion; may play a role in S-phase checkpoint control; has ubiquitin-associated (UBA), ubiquitin-like (UBL), and retroviral-like proteinase (RVP) domains
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cytoplasm 0.98 0.96 0.98 0.96 0.9 0.81 0.69 0.6 – 0.59 0.57 0.57 0.95 0.98 0.97 – 0.96 0.94 0.95 0.91 0.91 0.92 0.92 0.91
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.14 0.2 0.18 – 0.31 0.35 0.36 0 0 0 – 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.12 0.12 0.17 0.28 – 0.24 0.22 0.21 0.07 0 0 – 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 1 0 0 0 2 – 0 0 1 0 0 0 – 0 1 0 1 2 0 3 3
Bud 0 1 2 0 0 0 1 4 – 8 6 8 0 0 0 – 0 0 0 0 0 0 2 2
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0 0 0 0 0 0 1
Bud Site 0 0 0 0 0 0 0 0 – 0 1 0 0 0 0 – 0 0 – – – – – –
Cell Periphery 1 0 0 1 2 1 5 1 – 1 6 7 1 2 4 – 1 0 0 0 0 0 1 0
Cytoplasm 98 174 430 316 398 401 350 428 – 342 249 326 343 267 474 – 195 117 118 173 415 225 482 484
Endoplasmic Reticulum 1 4 0 0 0 1 6 4 – 6 0 13 7 2 9 – 6 4 0 1 6 3 9 10
Endosome 0 0 0 1 4 3 1 5 – 3 5 3 0 0 0 – 2 3 0 2 6 2 1 1
Golgi 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0 1 1 1 0 0 1
Mitochondria 0 5 6 11 17 72 102 132 – 178 152 210 0 0 0 – 7 1 0 1 5 2 8 9
Nucleus 1 1 0 0 3 1 14 19 – 17 13 16 7 9 9 – 2 0 0 2 1 0 1 3
Nuclear Periphery 0 0 0 0 2 1 1 4 – 6 4 2 0 0 0 – 0 0 0 1 0 0 0 0
Nucleolus 0 0 0 0 1 0 0 1 – 2 2 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 1 – 0 0 0 0 0 0 – 0 0 0 0 0 0 0 1
SpindlePole 0 0 0 0 0 0 0 0 – 0 1 0 0 0 0 – 2 1 0 0 1 1 0 1
Vac/Vac Membrane 1 1 12 14 55 60 87 202 – 142 95 122 24 3 12 – 4 4 2 2 6 5 8 6
Unique Cell Count 100 182 440 328 444 497 504 717 581 436 576 360 273 491 204 125 124 190 456 245 524 534
Labelled Cell Count 102 186 450 344 482 540 567 803 705 534 708 382 283 508 219 131 124 190 456 245 524 534


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.5 6.9 5.6 6.2 6.1 6.1 5.6 6.1 – 5.6 5.8 5.8 7.8 8.0 8.3 4.6 6.1 7.0 7.8 8.0 8.2
Std Deviation (1e-4) 0.7 1.1 0.9 1.1 1.1 1.2 1.2 1.2 – 1.1 1.3 1.2 0.9 0.9 0.9 0.9 1.2 1.5 1.6 1.2 1.3
Intensity Change (Log2) – – – 0.15 0.14 0.13 0.02 0.14 – 0.01 0.06 0.06 0.5 0.53 0.58 -0.26 0.12 0.32 0.48 0.52 0.57


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0
Bud 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0
Bud Neck 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0
Bud Site 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0
Cell Periphery 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0
Cytoplasm -1.1 -4.9 -8.2 -11.4 -14.3 – -14.3 -14.4 -14.9 -1.9 0.1 -1.1 – -1.5 -2.3
Endoplasmic Reticulum 0 0 0 0 0 – 0 0 3.2 0 0 0 – 0 0
Endosome 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0
Golgi 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0
Mitochondria 1.9 2.3 7.3 9.1 8.7 – 12.1 12.9 13.5 0 0 0 – 0 0
Nucleus 0 0 0 3.5 3.4 – 3.6 3.6 3.5 0 0 0 – 0 0
Nuclear Periphery 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0
Nucleolus 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0
Peroxisomes 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0
SpindlePole 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0
Vacuole 1.2 5.4 5.4 7.3 10.8 – 9.6 8.6 8.6 2.7 -1.5 -0.3 – -0.6 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration – – – – – – – – – – – – – – – – – –
Actin 0.0002 0.0002 0 0.0268 0 0.0012 0.0249 0.0007 0.0042 0.0003 0.0169 0.0043 0.0014 0.0033 0.0189 0.0006 0.0275 0.0116
Bud 0.0001 0 0 0.0004 0.0001 0 0.0019 0.0001 0.0002 0.0002 0.0002 0.0007 0.0001 0.0053 0.0003 0.0002 0.0005 0.0001
Bud Neck 0.0001 0.0001 0 0.0005 0.0001 0.0009 0.0006 0.0001 0.0004 0.0004 0.0006 0.0016 0.0003 0.0002 0.0013 0.0003 0.0019 0.0026
Bud Periphery 0.0001 0 0 0.0007 0 0.0001 0.0007 0 0.0001 0 0.0001 0.0005 0 0.0014 0.0004 0.0001 0.0007 0.0001
Bud Site 0.0002 0.0001 0 0.0009 0 0 0.0016 0.0006 0.0004 0.0001 0.0008 0.0009 0.0004 0.0072 0.0008 0.0002 0.0012 0.0001
Cell Periphery 0.0001 0 0.0001 0.0002 0 0.1245 0.0001 0.0001 0 0 0 0 0 0.0001 0.0001 0 0.0001 0
Cytoplasm 0.8538 0.7387 0.0057 0.0002 0.9832 0.7404 0.79 0.9277 0.8778 0.8526 0.8326 0.9012 0.8636 0.9328 0.8913 0.8858 0.8579 0.8811
Cytoplasmic Foci 0.0032 0.0658 0 0.0269 0.0022 0.006 0.0154 0.0072 0.0097 0.0051 0.0666 0.0093 0.0169 0.0023 0.0038 0.005 0.0097 0.015
Eisosomes 0 0 0 0.0007 0 0 0.0002 0 0 0 0.0002 0 0 0 0.0002 0 0.0001 0
Endoplasmic Reticulum 0.0067 0.001 0.9833 0.0004 0.0003 0.0835 0.0091 0.0029 0.0042 0.0028 0.005 0.0043 0.005 0.0027 0.0033 0.0059 0.0041 0.0041
Endosome 0.0026 0.0438 0.0002 0.035 0.0005 0.0137 0.0098 0.0056 0.0065 0.0029 0.0097 0.0035 0.0054 0.0014 0.0047 0.004 0.005 0.0082
Golgi 0.0001 0.0123 0 0.0991 0 0.0013 0.004 0.0004 0.0018 0.0001 0.0012 0.0005 0.0011 0.0002 0.0013 0.0002 0.0023 0.001
Lipid Particles 0.0001 0.1001 0.0001 0.1245 0 0.0033 0.0059 0.0007 0.0019 0 0.0014 0.001 0.0028 0.0001 0.0009 0.0002 0.0007 0.0022
Mitochondria 0.0002 0.0002 0 0.5596 0.0001 0.0002 0.0078 0.0004 0.0047 0.0002 0.0003 0.0003 0.0002 0.0002 0.001 0.0003 0.0003 0.0003
None 0.1232 0.0045 0 0.0004 0.0048 0.0189 0.0879 0.0423 0.0516 0.0729 0.0269 0.0417 0.0644 0.0295 0.0587 0.0811 0.074 0.0457
Nuclear Periphery 0.0026 0.0007 0.01 0.0003 0.0006 0.0024 0.0044 0.0018 0.0035 0.0046 0.0027 0.0017 0.0035 0.0014 0.0019 0.0024 0.0021 0.0033
Nucleolus 0.0001 0.0001 0 0.0003 0 0 0.0002 0.0001 0.0001 0.0001 0.0001 0 0.0002 0 0.0001 0 0.0001 0.0001
Nucleus 0.004 0.0041 0.0002 0.0002 0.0073 0.0017 0.019 0.0064 0.0236 0.0542 0.0258 0.0224 0.0219 0.0106 0.0081 0.0111 0.0072 0.0194
Peroxisomes 0 0.0261 0 0.119 0 0.0001 0.0117 0.0005 0.0058 0.0001 0.0041 0.0041 0.003 0.0001 0.0006 0.0001 0.002 0.0004
Punctate Nuclear 0.0004 0.0015 0 0.0006 0.0004 0.0005 0.0028 0.0008 0.002 0.0021 0.0038 0.0013 0.0089 0.0005 0.0006 0.001 0.0015 0.0042
Vacuole 0.0022 0.0004 0.0002 0.0011 0.0002 0.0011 0.0016 0.0012 0.001 0.0009 0.0009 0.0006 0.0007 0.0005 0.0015 0.001 0.0009 0.0006
Vacuole Periphery 0.0002 0.0001 0 0.0022 0.0001 0.0001 0.0003 0.0002 0.0002 0.0002 0.0002 0.0001 0.0001 0.0001 0.0002 0.0003 0.0002 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 15.0036 19.1604 15.924 21.9906 20.0086 15.4237 22.8602 28.2431 28.5298 18.3691
Translational Efficiency 2.2636 1.8884 2.3276 1.5666 1.4876 3.0369 1.8618 1.6773 1.751 1.8836

Hit Data

Dataset Hit
Protein Concentration –
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
109 1177 315 899 1475 685 2034 513 1584 1862 2349 1412

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1063.03 825.65 963.33 1021.98 759.71 822.59 957.28 1039.68 780.58 824.52 958.09 1028.41
Standard Deviation 156.42 151.31 121.78 172.15 96.53 111.06 160.12 184.80 127.50 137.88 155.54 177.06
Intensity Change Log 2 — -0.364580 -0.142080 -0.056815 — 0.114725 0.333492 0.452619 — -0.145182 0.075456 0.177698

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.001851 0.000443 0.000899 0.003404 0.000550 0.000518 0.000304 0.000721 0.000639 0.000471 0.000383 0.002429
Bud Neck 0.002173 0.004106 0.003279 0.008241 0.007632 0.010016 0.003564 0.004875 0.007256 0.006280 0.003526 0.007018
Bud Site 0.011446 0.009379 0.007689 0.032439 0.007990 0.012458 0.004777 0.046940 0.008228 0.010512 0.005167 0.037707
Cell Periphery 0.000234 0.000155 0.000274 0.000306 0.000136 0.000129 0.000085 0.000125 0.000142 0.000146 0.000110 0.000240
Cytoplasm 0.463957* 0.699443* 0.440298* 0.557466* 0.598340* 0.550592* 0.609696* 0.527781* 0.589093* 0.644683* 0.586980* 0.546681*
Cytoplasmic Foci 0.071307 0.111366 0.015960 0.022643 0.197680* 0.218845* 0.007328 0.030740 0.188984* 0.150906 0.008485 0.025585
Eisosomes 0.000073 0.000045 0.000056 0.000037 0.000077 0.000087 0.000017 0.000041 0.000076 0.000061 0.000022 0.000039
Endoplasmic Reticulum 0.003115 0.001526 0.008800 0.002343 0.002110 0.001353 0.002051 0.001425 0.002180 0.001462 0.002956 0.002010
Endosome 0.018290 0.008340 0.004112 0.008947 0.009496 0.023501 0.001217 0.009428 0.010101 0.013917 0.001605 0.009122
Golgi 0.016776 0.001352 0.000192 0.009902 0.002488 0.004704 0.000082 0.003939 0.003471 0.002585 0.000097 0.007735
Lipid Particles 0.002813 0.001994 0.002126 0.001532 0.004374 0.005479 0.000737 0.001590 0.004267 0.003276 0.000924 0.001553
Mitochondria 0.006370 0.001043 0.000368 0.008275 0.003731 0.006093 0.000510 0.001478 0.003913 0.002901 0.000491 0.005806
Mitotic Spindle 0.008075 0.004191 0.002485 0.040403 0.002015 0.006993 0.006013 0.058890 0.002432 0.005222 0.005540 0.047120
None 0.000667 0.006504 0.003507 0.008887 0.003286 0.001902 0.006975 0.010021 0.003105 0.004811 0.006510 0.009299
Nuclear Periphery 0.001076 0.000556 0.001229 0.000901 0.000393 0.000216 0.001453 0.000684 0.000440 0.000431 0.001422 0.000822
Nuclear Periphery Foci 0.005754 0.003222 0.007442 0.003836 0.001195 0.001890 0.002555 0.002338 0.001508 0.002732 0.003211 0.003292
Nucleolus 0.000103 0.001324 0.000306 0.000267 0.000900 0.000910 0.000269 0.000477 0.000846 0.001171 0.000274 0.000344
Nucleus 0.122870 0.078805 0.403207* 0.167799* 0.103167 0.066445 0.307462* 0.108293 0.104523 0.074258 0.320301* 0.146180*
Peroxisomes 0.006473 0.001317 0.000493 0.002753 0.002340 0.006934 0.000534 0.001593 0.002625 0.003383 0.000528 0.002331
Vacuole 0.255652* 0.063967 0.097010 0.116051 0.050920 0.079210 0.044136 0.186530 0.065008 0.069575 0.051226 0.141657
Vacuole Periphery 0.000924 0.000923 0.000269 0.003566 0.001180 0.001726 0.000237 0.002090 0.001163 0.001218 0.000241 0.003030

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 1.35 -0.36 -0.91 -2.42 -0.87 0.10 0.67 -0.14 -0.47 -2.42 0.41 0.43 -1.60 -2.46 -2.56
Bud Neck -2.16 -2.39 -4.21 -1.70 -1.26 -1.37 3.33 3.35 3.69 -0.39 0.85 3.14 1.58 0.65 -1.80
Bud Site -0.03 0.07 -2.93 -3.29 -2.98 -2.32 2.42 -2.69 -1.43 -3.58 -1.69 2.20 -4.58 -3.38 -6.13
Cell Periphery 0.18 -3.50 -1.52 -1.87 2.97 0.23 1.58 0.30 0.14 -3.97 -0.16 0.46 -2.04 -1.84 -4.47
Cytoplasm -10.76 -0.23 -1.43 23.87 -2.07 3.99 10.48 16.77 11.46 10.57 -6.07 10.89 18.83 25.32 10.33
Cytoplasmic Foci -7.61 4.42 4.41 22.76 -0.20 -2.32 36.85 32.75 26.06 -6.13 5.77 36.30 34.03 33.05 -8.24
Eisosomes 2.04 -0.51 2.55 -0.31 4.47 -1.17 8.09 3.56 5.03 -10.44 1.86 7.41 4.33 1.79 -8.87
Endoplasmic Reticulum 1.96 -5.75 0.46 -5.26 8.94 1.18 -3.91 0.08 -1.20 5.32 2.25 -7.03 -1.79 -4.28 7.20
Endosome 0.52 1.69 2.40 5.21 2.70 -5.59 11.41 6.38 8.21 -2.73 -3.41 11.07 7.77 9.60 -2.58
Golgi 1.28 1.55 1.03 -0.97 -2.23 -1.89 9.67 3.67 3.27 -2.16 0.42 7.29 1.45 1.05 -2.78
Lipid Particles 1.37 -0.16 0.12 -2.26 0.44 -0.96 9.76 3.43 2.57 -3.04 1.93 9.32 4.66 1.61 -5.85
Mitochondria 0.88 1.23 0.18 -1.05 -1.64 -0.90 3.60 3.56 2.30 0.02 0.71 3.76 1.89 1.04 -1.45
Mitotic Spindle 1.56 1.79 -1.49 -5.24 -5.61 -2.22 -3.96 -5.68 -4.60 -4.85 -1.97 -3.03 -7.89 -6.95 -6.77
None -3.30 -2.22 -4.58 0.14 -3.65 1.69 -4.26 -3.17 -4.23 -1.25 -1.46 -4.15 -4.05 -1.87 -1.43
Nuclear Periphery 2.06 -0.91 -1.26 -4.94 -0.91 2.37 -19.00 -12.04 -15.65 3.12 0.21 -18.76 -13.72 -9.03 5.20
Nuclear Periphery Foci 1.42 -1.54 0.91 -1.44 4.76 -1.74 -7.33 -4.93 -1.18 1.00 -3.15 -8.07 -6.61 -1.80 0.21
Nucleolus -3.01 -2.42 -1.50 2.81 1.73 0.04 1.64 1.50 1.54 -0.83 -0.56 1.58 1.63 3.20 0.47
Nucleus 1.85 -14.58 -6.03 -17.67 12.91 7.45 -42.67 -8.56 -12.21 18.22 7.49 -45.66 -15.45 -21.44 24.66
Peroxisomes 0.79 1.25 1.09 1.46 -0.87 -4.12 7.04 5.67 5.67 -1.42 -2.05 6.99 5.25 5.60 -1.74
Vacuole 11.00 8.21 3.72 -21.57 -10.23 -6.50 -8.98 -21.99 -19.01 -19.07 -0.09 -3.84 -27.45 -28.60 -25.79
Vacuole Periphery -1.43 0.78 -0.80 0.04 -1.19 -1.24 6.17 2.33 2.59 -1.06 -0.19 6.26 1.19 1.21 -1.59
Standard name
Human Ortholog
Description DNA damage-inducible v-SNARE binding protein; role in suppression of protein secretion; may play a role in S-phase checkpoint control; has ubiquitin-associated (UBA), ubiquitin-like (UBL), and retroviral-like proteinase (RVP) domains
Localization
Cell Percentages cytoplasm (85%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Ddi1

Ddi1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ddi1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available