Standard name
Human Ortholog
Description TATA-binding protein (TBP); general transcription factor that interacts with other factors to form the preinitiation complex at promoters; essential for viability, highly conserved; yeast gene can complement mutations in human homolog TBP
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06 0.43 – 0.3 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0.05 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Mitochondria 0 0.06 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0.09 – 0.05 0 0 0 0 0 0
Nucleus 0.9 0.87 0.91 0.88 0.81 0.74 0.73 0.66 0.66 0.64 0.63 0.61 0.89 0.85 0.77 0.35 – 0.6 0.79 0.88 0.84 0.58 0.43 0.38
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Nucleolus 0.37 0.2 0.2 0.2 0.22 0.42 0.36 0.41 0.52 0.5 0.55 0.54 0.24 0.18 0.24 0.13 – 0.2 0.14 0.06 0.12 0.26 0.36 0.38
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.17 – 0.05 0 0 0 0.07 0.12 0.14
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 1
Bud 0 0 1 0 0 2 0 2 2 0 1 5 0 0 0 0 – 0 0 2 1 3 1 2
Bud Neck 0 0 0 0 1 1 1 1 3 4 3 1 0 0 1 0 – 0 1 0 0 2 0 1
Bud Site 0 0 0 0 0 3 1 3 8 7 5 7 0 1 7 0 – 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Cytoplasm 1 13 4 13 14 9 13 23 14 11 7 9 6 16 20 10 – 6 1 1 1 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 – 1 0 0 0 0 1 0
Endosome 0 1 1 0 0 0 1 1 1 2 0 0 1 3 3 0 – 1 0 2 1 0 3 3
Golgi 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 – 0 0 0 1 0 1 2
Mitochondria 2 29 0 1 1 30 7 14 21 14 9 23 1 2 0 2 – 1 0 1 0 5 10 11
Nucleus 135 411 401 332 314 384 459 532 441 418 286 321 228 261 264 8 – 12 126 434 395 147 144 147
Nuclear Periphery 0 1 1 0 4 2 8 5 3 9 2 0 0 3 1 1 – 0 0 0 0 1 1 6
Nucleolus 55 93 90 76 86 215 224 333 350 327 252 286 62 56 83 3 – 4 21 27 58 67 121 147
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 1 1 2 0 – 0 0 0 0 0 0 0
SpindlePole 0 1 0 0 2 0 4 3 0 5 2 0 2 3 13 0 – 0 0 3 1 0 3 4
Vac/Vac Membrane 1 2 1 2 2 5 4 15 2 1 2 7 1 3 4 4 – 1 4 13 6 18 41 53
Unique Cell Count 150 472 440 379 386 517 628 808 671 655 457 528 257 307 341 23 20 160 491 470 257 339 391
Labelled Cell Count 194 551 499 424 424 651 724 933 845 798 569 659 302 349 398 29 26 160 491 470 257 339 391


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 19.7 16.0 16.8 16.4 15.9 10.8 14.0 13.3 12.2 12.9 12.6 12.2 18.5 17.9 17.2 18.6 – 11.0 14.9 15.6 16.0
Std Deviation (1e-4) 6.0 5.5 4.4 4.4 4.6 3.2 4.1 3.9 3.5 3.8 3.5 4.0 5.7 6.1 6.8 10.9 – 7.1 4.3 4.4 4.5
Intensity Change (Log2) – – – -0.04 -0.08 -0.64 -0.26 -0.33 -0.46 -0.38 -0.42 -0.46 0.14 0.09 0.04 0.15 – -0.61 -0.17 -0.11 -0.07


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0
Cytoplasm 2.5 2.7 1.1 1.5 2.2 1.5 1.1 0.8 1.1 0 3.6 4.0 0 – 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0
Mitochondria 0 0 5.1 0 0 3.7 3.1 0 4.4 0 0 0 0 – 0
Nucleus -1.6 -4.1 -6.8 -7.3 -9.8 -9.7 -10.2 -10.1 -10.8 -1.0 -2.6 -5.3 -8.2 – -4.5
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0
Nucleolus -0.1 0.6 7.0 5.4 7.4 10.6 9.8 10.7 10.7 1.1 -0.8 1.3 0 – 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 4.1 0 – 0
Vacuole 0 0 0 0 2.4 0 0 0 0 0 0 0 0 – 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 6.6177 12.3024 10.3252 10.531 9.0344 8.9641 15.0289 16.9235 15.7763 14.6413 14.4127 15.0702 16.1999 21.4222 19.4118 18.2189 16.2508 18.8717
Actin 0.028 0 0.0057 0.0273 0 0.0191 0.0005 0 0 0.001 0 0.0001 0.0367 0 0 0 0 0
Bud 0.0013 0 0.0007 0.0015 0 0.0017 0.0002 0 0 0.0003 0 0.0001 0.0003 0 0 0 0.0001 0
Bud Neck 0.001 0 0.0002 0.0004 0.0001 0.0023 0.0002 0 0 0.0001 0.0001 0.0003 0.005 0 0 0 0.0001 0.0002
Bud Periphery 0.0009 0 0.0008 0.0016 0.0001 0.0024 0.0002 0 0 0.0005 0.0001 0.0002 0.0006 0 0 0 0.0001 0.0001
Bud Site 0.0032 0 0.0011 0.0028 0.0001 0.0017 0.0005 0 0 0.0009 0.0001 0.0001 0.003 0 0 0 0.0001 0
Cell Periphery 0.0002 0 0.0003 0.0001 0.0001 0.0004 0 0 0 0.0001 0.0001 0.0001 0.0006 0 0 0 0.0003 0.0001
Cytoplasm 0.031 0 0.0019 0.0039 0 0.0051 0.0011 0 0 0.0001 0 0 0.0053 0 0 0 0.0001 0
Cytoplasmic Foci 0.0656 0 0.0052 0.0063 0 0.0141 0.0001 0 0 0.0013 0 0 0.0265 0 0 0 0.0003 0
Eisosomes 0.0003 0 0.0001 0.0002 0 0.0001 0 0 0 0.0001 0 0 0.0005 0 0 0 0 0
Endoplasmic Reticulum 0.0048 0 0.001 0.0042 0 0.0025 0.0004 0 0 0 0 0 0.0053 0 0 0 0 0
Endosome 0.0495 0 0.0076 0.0211 0 0.0326 0.0005 0 0 0.0004 0 0.0001 0.0168 0 0 0 0 0
Golgi 0.0112 0 0.001 0.0101 0 0.02 0 0 0 0.0004 0 0 0.0041 0 0 0 0 0
Lipid Particles 0.0365 0 0.0036 0.0039 0 0.0217 0 0 0 0.0006 0 0 0.0122 0 0 0 0.0005 0
Mitochondria 0.007 0.0003 0.0137 0.0276 0.0004 0.0156 0.0019 0.0003 0.0002 0.033 0.0018 0.0014 0.0025 0.0011 0.0004 0.0002 0.0048 0.0002
None 0.0159 0 0.0032 0.0017 0 0.0007 0.0003 0 0 0.0003 0 0 0.0051 0 0 0 0.0003 0
Nuclear Periphery 0.0471 0.0003 0.0085 0.0708 0.001 0.0232 0.0185 0.0009 0.0004 0.0021 0.0001 0.0046 0.0322 0.0004 0.0005 0.0105 0.0043 0
Nucleolus 0.0504 0.0335 0.0643 0.0183 0.2924 0.1686 0.0404 0.016 0.0212 0.0879 0.433 0.1356 0.0851 0.0441 0.0372 0.0554 0.2741 0.1014
Nucleus 0.4773 0.9497 0.8708 0.7728 0.7048 0.6432 0.928 0.9821 0.9781 0.8418 0.5638 0.856 0.731 0.9537 0.9615 0.9332 0.7102 0.8976
Peroxisomes 0.0152 0 0.0012 0.0047 0 0.0093 0 0 0 0.0007 0 0 0.0038 0 0 0 0.0001 0
Punctate Nuclear 0.1486 0.016 0.0037 0.0093 0.0002 0.0064 0.006 0.0001 0 0.0277 0.0002 0.0001 0.0179 0 0 0.0003 0.0014 0.0001
Vacuole 0.0024 0 0.0022 0.0017 0.0004 0.0043 0.0005 0.0001 0 0.0002 0.0002 0.0003 0.0024 0.0001 0.0001 0 0.0006 0.0001
Vacuole Periphery 0.0027 0 0.0033 0.0098 0.0003 0.0049 0.0007 0.0003 0.0001 0.0007 0.0004 0.001 0.0029 0.0005 0.0001 0.0002 0.0025 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 90.8058 102.3212 90.0997 95.292 102.8933 67.1133 85.8692 79.6021 89.4211 92.9837
Translational Efficiency 1.5728 1.2257 1.3397 1.1706 1.3353 1.9414 1.2779 1.3517 1.2691 1.3749

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
112 869 1880 1309 1845 2697 2369 159 1957 3566 4249 1468

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1326.73 1329.60 1698.25 1472.38 1110.51 1514.36 1727.92 1833.35 1122.88 1469.34 1714.79 1511.48
Standard Deviation 284.84 382.68 334.28 653.68 384.43 401.94 354.87 425.27 382.74 405.17 346.23 642.79
Intensity Change Log 2 — 0.003117 0.356174 0.150275 — 0.447486 0.637814 0.723260 — 0.222653 0.491348 0.439721

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000885 0.000663 0.000581 0.001106 0.000726 0.000639 0.000578 0.001174 0.000735 0.000645 0.000580 0.001113
Bud Neck 0.005759 0.005023 0.009032 0.020473 0.005803 0.007355 0.007814 0.021681 0.005801 0.006787 0.008353 0.020604
Bud Site 0.000826 0.000483 0.000534 0.004870 0.000811 0.000772 0.000507 0.002072 0.000812 0.000702 0.000519 0.004567
Cell Periphery 0.000025 0.000035 0.000024 0.000516 0.000142 0.000110 0.000072 0.000059 0.000135 0.000092 0.000051 0.000466
Cytoplasm 0.000156 0.000103 0.000026 0.005435 0.000292 0.000265 0.000024 0.000196 0.000285 0.000226 0.000025 0.004867
Cytoplasmic Foci 0.000395 0.000112 0.000038 0.001540 0.000080 0.000179 0.000023 0.001680 0.000098 0.000163 0.000030 0.001555
Eisosomes 0.000076 0.000071 0.000100 0.000047 0.000085 0.000091 0.000140 0.000085 0.000084 0.000086 0.000122 0.000051
Endoplasmic Reticulum 0.001159 0.000436 0.000304 0.001397 0.001240 0.000667 0.000493 0.000599 0.001235 0.000610 0.000409 0.001311
Endosome 0.000043 0.000294 0.000006 0.002517 0.000087 0.000072 0.000023 0.003749 0.000084 0.000126 0.000015 0.002650
Golgi 0.000214 0.000092 0.000030 0.001426 0.000065 0.000079 0.000020 0.001923 0.000074 0.000082 0.000024 0.001480
Lipid Particles 0.000112 0.000020 0.000002 0.000234 0.000009 0.000037 0.000002 0.000105 0.000015 0.000033 0.000002 0.000220
Mitochondria 0.001142 0.001596 0.001478 0.006737 0.001724 0.001475 0.001251 0.006015 0.001690 0.001505 0.001351 0.006659
Mitotic Spindle 0.009919 0.000416 0.000066 0.010889 0.002128 0.001305 0.000032 0.010625 0.002574 0.001089 0.000047 0.010861
None 0.000673 0.000930 0.000385 0.005186 0.002952 0.002220 0.000332 0.000944 0.002821 0.001906 0.000356 0.004727
Nuclear Periphery 0.000054 0.000056 0.000053 0.001674 0.000369 0.000150 0.000090 0.001343 0.000351 0.000127 0.000074 0.001638
Nuclear Periphery Foci 0.000288 0.000274 0.000003 0.001234 0.000157 0.000104 0.000008 0.000430 0.000164 0.000145 0.000006 0.001147
Nucleolus 0.065070 0.069960 0.030974 0.026169 0.043933 0.038510 0.026478 0.117619 0.045143 0.046174 0.028467 0.036074
Nucleus 0.903293* 0.915790* 0.954468* 0.897372* 0.937344* 0.944372* 0.960219* 0.816752* 0.935395* 0.937407* 0.957674* 0.888640*
Peroxisomes 0.009494 0.002521 0.001578 0.001134 0.001398 0.001130 0.001100 0.004958 0.001861 0.001469 0.001312 0.001548
Vacuole 0.000274 0.000443 0.000128 0.005132 0.000309 0.000210 0.000392 0.004411 0.000307 0.000267 0.000275 0.005054
Vacuole Periphery 0.000142 0.000681 0.000190 0.004912 0.000347 0.000259 0.000401 0.003579 0.000335 0.000361 0.000308 0.004768

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 1.55 1.66 -0.32 -7.25 -7.81 1.76 3.02 -6.98 -8.17 -8.95 2.27 3.43 -6.28 -8.28 -9.13
Bud Neck 1.30 -3.68 -8.00 -9.45 -6.83 -4.30 -5.52 -4.33 -3.95 -3.79 -3.06 -8.46 -9.88 -9.29 -8.11
Bud Site 1.37 1.12 -3.96 -5.58 -5.43 0.08 1.28 -2.83 -2.95 -3.33 0.61 1.35 -5.27 -5.56 -5.76
Cell Periphery -0.29 0.74 -3.23 -3.21 -3.28 0.99 1.98 2.66 2.04 0.19 1.57 3.09 -2.43 -2.78 -3.09
Cytoplasm 0.73 1.13 -4.84 -5.07 -5.15 0.11 5.76 0.76 0.32 -3.68 0.54 5.83 -4.87 -4.92 -5.17
Cytoplasmic Foci 1.46 1.85 -2.93 -4.42 -4.69 -1.57 2.79 -1.54 -1.42 -1.60 -1.28 2.94 -4.75 -4.48 -4.99
Eisosomes 0.76 -2.91 3.20 3.59 8.79 -0.36 -2.99 -0.31 0.08 5.18 -0.12 -2.35 1.85 6.21 11.53
Endoplasmic Reticulum 2.27 2.53 0.03 -5.91 -6.65 7.57 5.65 2.65 -0.28 -1.07 8.91 9.13 -0.52 -4.79 -5.71
Endosome -1.13 1.78 -3.33 -2.86 -3.42 0.63 4.49 -1.34 -1.34 -1.37 -0.83 6.42 -3.57 -3.49 -3.68
Golgi 1.55 2.29 -2.47 -2.91 -3.07 -0.50 7.32 -1.30 -1.29 -1.34 -0.34 6.08 -3.24 -3.21 -3.36
Lipid Particles 1.50 1.74 -0.59 -2.94 -3.28 -1.01 2.82 -2.35 -1.34 -2.53 -0.74 2.68 -3.19 -2.77 -3.46
Mitochondria -2.66 -2.96 -6.96 -6.51 -6.47 1.16 3.41 -6.63 -6.84 -7.30 1.09 2.21 -6.98 -7.18 -7.36
Mitotic Spindle 1.82 1.86 0.22 -5.03 -5.20 0.90 3.12 -1.69 -1.84 -2.13 1.93 3.57 -4.01 -5.00 -5.61
None -1.51 2.82 -5.15 -4.95 -5.58 0.98 4.80 3.27 2.14 -7.89 1.43 4.77 -1.97 -3.24 -5.74
Nuclear Periphery 0.49 0.13 -4.85 -4.88 -4.86 5.27 5.84 -1.23 -1.51 -1.59 5.94 7.06 -4.13 -4.89 -5.06
Nuclear Periphery Foci -0.17 2.16 -2.13 -1.40 -3.52 0.17 3.26 -1.09 -1.11 -1.85 -0.36 3.70 -3.05 -2.73 -3.65
Nucleolus -0.10 2.88 3.22 9.10 1.37 1.77 6.67 -6.42 -6.82 -7.77 -0.47 6.25 1.34 1.85 -3.63
Nucleus -1.19 -2.99 0.13 3.43 9.07 -1.80 -7.06 7.25 7.60 8.47 -0.57 -6.66 8.39 9.08 12.35
Peroxisomes 2.06 2.22 2.36 3.66 2.05 1.43 1.24 -6.01 -6.50 -6.44 1.52 1.38 -0.03 -2.33 -2.06
Vacuole -0.52 2.05 -4.83 -4.62 -5.06 1.66 -0.50 -1.82 -1.87 -1.78 0.57 0.28 -5.18 -5.23 -5.20
Vacuole Periphery -1.48 -1.28 -4.03 -3.37 -3.99 1.36 -0.34 -1.54 -1.60 -1.51 -0.21 0.28 -4.09 -4.05 -4.11
Standard name
Human Ortholog
Description TATA-binding protein (TBP); general transcription factor that interacts with other factors to form the preinitiation complex at promoters; essential for viability, highly conserved; yeast gene can complement mutations in human homolog TBP
Localization
Cell Percentages nucleus (99%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-2

Spt15

Spt15


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Spt15-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available