ORF
Human Ortholog
Description Protein with 2-aminoadipate transaminase activity; shares amino acid similarity with the aminotransferases Aro8p and Aro9p; YER152C is not an essential gene
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.98 0.97 0.97 0.94 0.92 0.85 0.74 0.69 0.66 0.6 0.62 0.62 1.0 0.99 0.98 0.91 0.93 0.94 0.93 0.94 0.9 0.95 0.94 0.93
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0.06 0 0.16 0.25 0.22 0.28 0.36 0.44 0.38 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0.06 0.08 0.08 0.1 0.15 0.16 0.2 0.12 0.09 0 0 0 0.12 0.06 0.06 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0
Bud 1 0 0 0 2 0 2 4 7 11 0 4 0 0 0 0 0 0 3 0 5 0 1 3
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 5 1 1 2 6 10 2 5 0 4 0 0 0 7 1 6 0 0 0 0 0 0
Cytoplasm 291 114 299 222 326 327 402 401 319 340 130 213 20 189 239 277 258 187 279 117 284 62 213 172
Endoplasmic Reticulum 2 0 2 0 1 0 3 3 9 4 1 7 0 0 2 8 10 6 1 0 3 0 0 0
Endosome 0 0 0 0 0 0 0 1 2 0 0 1 0 0 0 0 2 0 1 0 3 0 0 0
Golgi 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 1 0 0 0
Mitochondria 6 1 3 13 14 63 138 127 136 202 93 131 0 0 0 1 2 0 1 0 2 0 1 0
Nucleus 2 1 1 2 3 3 16 18 16 13 4 11 0 1 4 11 7 2 1 2 0 2 1 3
Nuclear Periphery 0 0 0 0 0 1 0 1 1 2 1 4 0 0 0 0 0 0 0 0 1 0 2 0
Nucleolus 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 1 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0
Vac/Vac Membrane 1 3 5 13 27 29 53 86 77 115 26 30 0 2 0 35 16 12 4 0 4 0 2 1
Unique Cell Count 296 118 307 236 354 386 545 584 486 565 211 345 20 190 243 304 276 198 300 125 315 66 227 185
Labelled Cell Count 303 119 316 251 374 425 620 651 570 693 255 405 20 192 245 339 297 214 300 125 315 66 227 185


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.8 8.0 7.4 7.0 7.2 6.4 6.3 6.2 5.8 5.6 5.3 5.5 7.0 8.4 8.1 14.0 13.3 13.7 7.1 8.2 8.7
Std Deviation (1e-4) 1.3 1.6 1.3 1.8 1.7 1.2 1.3 1.3 1.2 1.2 1.3 1.2 0.9 1.3 1.1 2.7 2.9 3.1 0.8 1.4 1.6
Intensity Change (Log2) – – – -0.1 -0.06 -0.22 -0.25 -0.27 -0.35 -0.41 -0.48 -0.44 -0.09 0.18 0.12 0.91 0.84 0.88 -0.06 0.14 0.23


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0.1 0 0 0 0 0 0 0 0.6 0 0
Cytoplasm -1.9 -3.0 -5.6 -8.7 -9.9 -10.5 -11.9 -10.6 -11.1 0.7 1.7 0.8 -3.3 -2.3 -1.7
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 2.5 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 3.1 2.4 6.8 9.2 8.3 9.7 11.6 12.4 11.7 0 0 0 0 0 0
Nucleus 0 0 0 2.6 2.7 2.8 0 0 2.7 0 0 0 2.9 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 2.5 3.6 3.6 4.5 6.1 6.4 7.7 5.0 4.0 0 0 0 4.9 2.7 2.7

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.6935 3.2694 3.2958 2.5324 2.3646 3.0306 2.2458 2.2398 1.8574 1.9744 1.5912 2.0078 1.2089 2.0489 1.1524 1.2097 1.4162 1.2415
Actin 0.008 0.0005 0.0017 0.0004 0.0003 0.0022 0.0471 0.001 0.0159 0.0065 0.0052 0.0076 0.0004 0.0002 0.0006 0.0002 0.0005 0.0004
Bud 0.0002 0.0042 0.0002 0.0002 0.0001 0.0004 0.0033 0.0008 0.0011 0.0009 0.0009 0.0004 0.0001 0.0001 0.0003 0.0003 0.0002 0.0001
Bud Neck 0.0014 0.0004 0.0013 0.0004 0.0003 0.0009 0.0147 0.0003 0.0009 0.0013 0.0006 0.0052 0.0001 0.0001 0.0014 0.0005 0.0005 0.0044
Bud Periphery 0.0003 0.0023 0.0001 0.0002 0.0001 0.0005 0.0047 0.0004 0.0011 0.0006 0.0007 0.0005 0.0001 0 0.0001 0.0002 0.0001 0
Bud Site 0.0009 0.0107 0.0022 0.0001 0.0001 0.0002 0.0089 0.0065 0.0037 0.0056 0.0006 0.0004 0.0001 0.0005 0.0011 0.0001 0.0001 0.0001
Cell Periphery 0.0001 0.0003 0.0001 0 0 0 0.0004 0.0001 0.0002 0.0002 0.0001 0.0001 0 0 0 0 0 0
Cytoplasm 0.6392 0.7646 0.7403 0.7305 0.8085 0.759 0.5479 0.8676 0.8039 0.8259 0.8505 0.8644 0.8111 0.8914 0.8671 0.8702 0.8732 0.8458
Cytoplasmic Foci 0.0096 0.0066 0.0051 0.0096 0.0077 0.008 0.0354 0.0092 0.017 0.0283 0.0154 0.0165 0.005 0.0036 0.005 0.0079 0.0226 0.0072
Eisosomes 0.0003 0 0.0001 0 0 0 0.0003 0 0.0002 0.0001 0.0001 0 0 0 0 0 0 0
Endoplasmic Reticulum 0.0036 0.0018 0.002 0.0015 0.0024 0.0019 0.0092 0.0023 0.0057 0.0042 0.0033 0.0046 0.0043 0.0029 0.0022 0.0021 0.0032 0.002
Endosome 0.0029 0.0017 0.0017 0.0032 0.0036 0.0047 0.0554 0.0031 0.0127 0.0238 0.0116 0.0186 0.0056 0.0021 0.0022 0.0072 0.0091 0.0064
Golgi 0.0011 0.0002 0.0003 0.0002 0.0002 0.0002 0.0155 0.0005 0.0025 0.0078 0.003 0.0056 0.0012 0.0001 0.0001 0.0001 0.0003 0.0007
Lipid Particles 0.0018 0.0001 0.0005 0.0001 0.0001 0.0002 0.0105 0.0015 0.0023 0.0082 0.015 0.004 0.0003 0 0.0001 0 0.0002 0.0034
Mitochondria 0.0005 0.0002 0.0004 0.0003 0.0003 0.0002 0.0158 0.0006 0.0034 0.0044 0.0023 0.002 0.0044 0.0002 0.0002 0.0004 0.0003 0.0008
None 0.3185 0.1997 0.2354 0.2435 0.1678 0.2131 0.1444 0.0917 0.1003 0.0549 0.0427 0.0448 0.1466 0.0895 0.1014 0.0781 0.0622 0.1058
Nuclear Periphery 0.0012 0.0006 0.0009 0.0007 0.0013 0.0009 0.0315 0.0014 0.008 0.0028 0.0112 0.0039 0.003 0.0017 0.0015 0.0027 0.0023 0.0017
Nucleolus 0.0007 0.0001 0.0002 0.0001 0 0.0001 0.0015 0.0001 0.0006 0.0004 0.0005 0.0001 0.0002 0 0.0001 0.0001 0.0001 0.001
Nucleus 0.0041 0.0039 0.0046 0.0067 0.005 0.0034 0.0206 0.0044 0.0098 0.0093 0.0186 0.0128 0.0128 0.0057 0.0141 0.0262 0.0158 0.016
Peroxisomes 0.0029 0.0001 0.0012 0.0002 0.0001 0.0002 0.0089 0.005 0.0021 0.0051 0.01 0.0029 0.0003 0 0.0001 0.0001 0.0002 0.0006
Punctate Nuclear 0.0019 0.001 0.0009 0.001 0.0011 0.0031 0.0104 0.0022 0.0054 0.0066 0.0038 0.0027 0.003 0.0006 0.0013 0.001 0.0068 0.0026
Vacuole 0.0007 0.001 0.0006 0.0007 0.0007 0.0007 0.0082 0.0013 0.0024 0.0024 0.003 0.0022 0.0011 0.0009 0.0009 0.002 0.002 0.0009
Vacuole Periphery 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0.0054 0.0002 0.0008 0.0007 0.0011 0.0007 0.0003 0.0001 0.0002 0.0005 0.0004 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 49.2887 37.7667 24.7275 35.9834 58.5348 37.2565 35.378 31.5844 39.964 62.0977
Translational Efficiency 0.4464 0.5098 0.4622 0.4871 0.4933 0.5914 0.5057 0.724 0.454 0.4483

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
68 413 1415 107 1662 1761 138 95 1730 2174 1553 202

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 985.41 882.83 1040.08 1099.77 871.42 866.27 1129.10 1072.10 875.90 869.42 1047.99 1086.76
Standard Deviation 187.50 137.33 181.50 139.27 118.16 130.71 170.31 188.19 123.63 132.15 182.31 164.68
Intensity Change Log 2 — -0.158588 0.077898 0.158406 — -0.008551 0.373733 0.298999 — -0.086229 0.224308 0.226096

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.003869 0.000459 0.000410 0.000490 0.000138 0.000511 0.000300 0.000519 0.000285 0.000501 0.000400 0.000504
Bud Neck 0.004317 0.006399 0.002838 0.001916 0.002459 0.004317 0.002106 0.003212 0.002532 0.004712 0.002773 0.002526
Bud Site 0.038546 0.018410 0.008450 0.005149 0.003762 0.008984 0.003723 0.005081 0.005129 0.010774 0.008030 0.005117
Cell Periphery 0.001056 0.000113 0.000110 0.000178 0.000096 0.000064 0.000339 0.000181 0.000134 0.000073 0.000131 0.000179
Cytoplasm 0.468608* 0.663596* 0.517487* 0.443344* 0.693051* 0.696941* 0.320265* 0.582534* 0.684229* 0.690606* 0.499962* 0.508805*
Cytoplasmic Foci 0.153655 0.115937 0.005536 0.019022 0.095113 0.089760 0.044344 0.013526 0.097414 0.094733 0.008984 0.016438
Eisosomes 0.000099 0.000043 0.000016 0.000068 0.000035 0.000030 0.000154 0.000035 0.000037 0.000033 0.000028 0.000052
Endoplasmic Reticulum 0.035920 0.001105 0.002307 0.004476 0.003010 0.000599 0.004358 0.001909 0.004304 0.000695 0.002489 0.003268
Endosome 0.010343 0.012524 0.001041 0.003283 0.004357 0.006810 0.009850 0.006649 0.004592 0.007896 0.001824 0.004866
Golgi 0.005950 0.001706 0.000094 0.000093 0.000783 0.002990 0.000131 0.000676 0.000987 0.002746 0.000097 0.000367
Lipid Particles 0.030150 0.002486 0.000602 0.002400 0.001883 0.001260 0.004835 0.002108 0.002994 0.001493 0.000978 0.002263
Mitochondria 0.008064 0.001328 0.000493 0.000156 0.001194 0.003671 0.000198 0.005937 0.001464 0.003226 0.000467 0.002875
Mitotic Spindle 0.004964 0.002821 0.005563 0.002644 0.002116 0.016143 0.000496 0.004846 0.002228 0.013612 0.005113 0.003680
None 0.003981 0.003877 0.006175 0.006124 0.003249 0.003140 0.009828 0.018480 0.003278 0.003280 0.006499 0.011935
Nuclear Periphery 0.001166 0.000205 0.001030 0.001117 0.000349 0.000275 0.001576 0.002390 0.000381 0.000262 0.001079 0.001716
Nuclear Periphery Foci 0.014540 0.001909 0.001992 0.005958 0.001610 0.001275 0.005662 0.004595 0.002118 0.001395 0.002318 0.005317
Nucleolus 0.007141 0.000595 0.000160 0.000115 0.000331 0.000752 0.001182 0.000233 0.000598 0.000722 0.000251 0.000170
Nucleus 0.119010 0.064166 0.401711* 0.160512* 0.133980* 0.092433 0.311378* 0.101589 0.133392* 0.087063 0.393684* 0.132801*
Peroxisomes 0.011323 0.003853 0.000142 0.000210 0.000697 0.002640 0.000642 0.000353 0.001115 0.002870 0.000186 0.000277
Vacuole 0.068715 0.097644 0.043735 0.342622* 0.051275 0.066470 0.278361* 0.226512* 0.051960 0.072392 0.064584 0.288016*
Vacuole Periphery 0.008581 0.000824 0.000111 0.000123 0.000512 0.000934 0.000274 0.018635 0.000829 0.000913 0.000126 0.008829

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 4.03 3.91 3.62 -2.70 -1.70 -1.67 -5.34 -4.97 -0.02 -1.11 -1.10 -2.54 -4.51 -0.93 -1.09
Bud Neck -0.69 1.66 1.93 2.56 0.45 -3.76 0.69 -0.84 2.74 -1.36 -4.51 -1.81 -1.27 3.29 0.75
Bud Site 1.65 2.55 2.57 3.61 0.11 -4.67 -0.97 -3.23 -0.04 -2.56 -4.68 -1.49 -2.49 1.91 -0.56
Cell Periphery 4.57 4.51 4.12 -3.39 -3.12 5.52 -8.40 -3.80 -6.07 5.25 5.66 -0.50 -2.51 -7.20 -2.48
Cytoplasm -5.42 -0.11 4.07 13.75 7.14 0.40 21.01 11.43 11.28 -2.04 0.16 28.56 19.00 18.95 6.27
Cytoplasmic Foci 1.56 7.78 7.31 13.52 -4.28 1.20 12.07 21.35 20.35 4.07 0.61 28.01 24.35 25.49 -4.40
Eisosomes 3.49 4.86 1.31 -5.13 -9.65 1.87 -10.71 -4.77 -5.62 7.75 1.90 1.15 -7.73 -8.98 -8.43
Endoplasmic Reticulum 4.07 3.84 3.52 -6.17 -3.56 3.56 -3.10 0.95 -5.84 5.65 4.81 1.25 0.18 -8.03 -1.75
Endosome -0.69 6.13 4.61 4.49 -5.28 -3.60 -4.61 3.06 5.25 5.90 -4.73 8.40 3.86 7.05 -2.47
Golgi 2.78 4.10 4.08 2.57 -1.21 -3.49 3.45 3.01 4.68 -0.70 -3.24 4.59 4.27 5.20 -1.42
Lipid Particles 2.53 2.68 2.48 -0.60 -6.44 2.31 -5.99 -0.49 -2.67 5.54 2.76 3.57 0.74 -3.42 -6.13
Mitochondria 2.15 2.41 2.51 1.58 5.65 -2.37 1.85 0.46 2.54 -0.87 -1.90 1.77 1.55 3.40 0.08
Mitotic Spindle 1.22 0.53 -0.79 -1.53 -1.16 -5.50 1.31 -1.21 0.98 -1.43 -5.26 -2.29 -1.95 0.13 -1.38
None 0.12 -1.57 1.29 1.30 4.06 0.36 -0.90 -2.03 -2.21 -0.03 0.20 -4.18 -0.82 -0.99 1.77
Nuclear Periphery 2.90 -0.75 -2.43 -8.83 -3.21 0.96 -10.22 -5.37 -5.43 1.79 1.84 -21.23 -9.38 -9.64 -1.94
Nuclear Periphery Foci 5.03 4.87 2.84 -4.77 -4.42 1.21 -6.94 -4.39 -4.76 2.40 2.73 -2.39 -5.45 -6.57 -4.59
Nucleolus 1.92 2.05 2.06 3.22 1.34 -1.56 -2.60 4.83 2.22 3.54 -0.51 2.48 3.18 2.58 3.64
Nucleus 3.06 -14.94 -2.09 -6.22 12.95 8.96 -7.42 0.16 -2.49 5.97 10.66 -38.04 -1.93 -5.71 18.91
Peroxisomes 1.57 2.34 2.30 4.62 -1.08 -4.62 0.22 3.95 6.05 2.29 -4.18 4.02 3.39 6.99 -0.77
Vacuole -1.07 0.46 -11.68 -12.69 -14.11 -6.18 -12.91 -12.07 -11.38 -2.14 -7.92 -10.92 -19.20 -17.83 -16.35
Vacuole Periphery 1.76 1.92 1.92 6.41 0.11 -1.93 3.62 -0.79 -0.61 -0.92 -0.32 3.87 -0.29 -0.22 -0.97
ORF
Human Ortholog
Description Protein with 2-aminoadipate transaminase activity; shares amino acid similarity with the aminotransferases Aro8p and Aro9p; YER152C is not an essential gene
Localization
Cell Percentages cytoplasm (98%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

YER152C

YER152C


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Yer152c-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available