Standard name
Human Ortholog
Description Mitochondrial translational activator specific for the COX3 mRNA; acts together with Pet54p and Pet494p; located in the mitochondrial inner membrane
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0.05 0 0 0 0.05 0.07 0.05 0.05 0 0 0 – 0 0 0 0 0 0 0 0
Bud Neck 0 0 0.09 0 0.05 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0.05 0 0 0 0 0 0
Cytoplasm 0.18 0.54 0.59 0.46 0.42 0.32 0.22 0.22 0.12 0.13 0.09 0.13 0.76 0.92 0.95 – 0.31 0.39 0 0.12 0.06 0.09 0.14 0.11
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0.08 0.07 0 0 0 0 0 0
Endosome 0.08 0 0 0.07 0 0.05 0.08 0.06 0 0 0 0 0.05 0.05 0 – 0.2 0.13 0.11 0.06 0.09 0 0.05 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 0.64 0.37 0.06 0.34 0.47 0.56 0.56 0.58 0.87 0.8 0.86 0.84 0.19 0 0 – 0.14 0.08 0.66 0.61 0.67 0.66 0.65 0.61
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 – 0.13 0 0 0 0 0 0 0
SpindlePole 0.05 0 0.22 0.18 0.12 0.08 0.19 0.17 0.07 0.15 0.1 0.1 0 0 0 – 0.19 0.15 0 0 0 0 0 0
Vac/Vac Membrane 0.16 0.07 0 0 0 0.07 0.06 0.08 0 0 0 0 0 0 0 – 0.09 0.19 0.07 0.07 0 0.06 0.06 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 1 4 3 1 1 0 2 0 0 0 2 1 1 0 – 2 3 9 18 9 3 1 6
Bud 6 5 4 4 8 13 5 7 20 23 19 21 2 0 0 – 4 4 4 3 0 0 0 1
Bud Neck 1 4 15 3 8 7 5 5 0 1 2 1 1 0 0 – 0 3 5 5 1 2 0 5
Bud Site 0 0 0 0 3 0 1 0 9 1 5 8 0 0 0 – 0 0 – – – – – –
Cell Periphery 2 2 1 4 0 0 0 1 6 2 2 1 0 0 2 – 8 8 1 2 1 0 0 1
Cytoplasm 61 210 95 94 74 95 84 73 50 41 35 55 47 119 122 – 56 61 7 67 17 11 20 22
Endoplasmic Reticulum 7 1 3 2 1 1 3 1 0 3 0 1 0 0 1 – 14 11 3 4 2 0 0 2
Endosome 28 12 1 15 1 16 30 20 1 6 8 8 3 6 0 – 36 21 48 33 24 4 7 9
Golgi 0 0 0 0 0 0 1 0 0 0 0 0 0 1 0 – 1 3 6 4 1 1 0 3
Mitochondria 211 144 9 70 82 166 210 194 365 258 323 367 12 0 2 – 26 13 300 347 189 77 97 128
Nucleus 4 3 0 0 3 11 12 11 11 8 8 16 2 0 0 – 4 4 0 2 0 0 1 0
Nuclear Periphery 1 0 0 0 0 0 0 1 1 2 0 3 0 0 0 – 1 0 2 2 0 1 0 0
Nucleolus 1 1 0 0 0 0 0 1 1 0 3 1 0 0 1 – 1 0 2 1 0 0 1 0
Peroxisomes 3 1 1 10 0 1 5 6 2 1 2 7 0 2 0 – 24 6 7 3 8 0 0 3
SpindlePole 17 16 36 37 21 24 70 56 31 47 36 42 2 2 0 – 34 23 15 15 8 2 5 8
Vac/Vac Membrane 53 28 3 2 4 20 24 27 12 11 13 18 1 1 3 – 16 30 29 40 6 6 9 11
Unique Cell Count 331 386 162 203 175 296 377 335 420 323 375 436 62 129 128 181 157 452 567 282 118 151 209
Labelled Cell Count 396 428 172 244 206 355 450 405 509 404 456 551 71 132 131 227 190 452 567 282 118 151 209


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.3 3.8 4.8 4.7 4.4 4.2 4.4 4.6 3.7 4.1 3.8 3.9 4.8 5.1 4.8 4.2 5.9 5.8 5.5 5.7 5.9
Std Deviation (1e-4) 0.6 0.6 1.0 1.5 1.0 1.3 1.4 1.4 1.1 0.9 1.0 1.1 1.1 1.2 1.3 1.1 1.9 1.6 1.5 1.8 1.4
Intensity Change (Log2) – – – -0.04 -0.13 -0.17 -0.12 -0.06 -0.39 -0.24 -0.32 -0.28 0.02 0.1 -0.01 -0.18 0.3 0.28 0.2 0.26 0.3


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – 0 0
Bud Neck – – – – – – – – – – – – – 0 0
Bud Site – – – – – – – – – – – – – 0 0
Cell Periphery – – – – – – – – – – – – – 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – 0 0
Endosome – – – – – – – – – – – – – 0 0
Golgi – – – – – – – – – – – – – 0 0
Mitochondria – – – – – – – – – – – – – 0 0
Nuclear Periphery – – – – – – – – – – – – – 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – 0 0
Peroxisomes – – – – – – – – – – – – – 0 0
SpindlePole – – – – – – – – – – – – – 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – 0 0
Cytoplasm – – – – – – – – – – – – – 0 0
Nucleus – – – – – – – – – – – – – 0 0
Vacuole – – – – – – – – – – – – – 2.8 5.1

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.281 -0.3266 -0.2811 -0.5507 -0.7364 -0.2774 5.1151 5.3508 5.3409 5.1481 6.1321 5.8829 2.4272 1.7369 2.5421 2.326 2.1493 2.3691
Actin 0.052 0.0072 0.015 0.0053 0.0085 0.0133 0.0328 0.0343 0.0386 0.0059 0.0536 0.0161 0.0204 0.0198 0.0225 0.0019 0.0498 0.0196
Bud 0.0014 0.0017 0.0034 0.0016 0.0017 0.0008 0.0018 0.0051 0.0026 0.0036 0.0039 0.0008 0.0006 0.0025 0.0017 0.0018 0.0044 0.0009
Bud Neck 0.003 0.0022 0.0061 0.0008 0.0016 0.0033 0.0019 0.0021 0.0049 0.0004 0.002 0.0081 0.0012 0.0015 0.0073 0.0004 0.0018 0.0139
Bud Periphery 0.0027 0.0035 0.0137 0.0076 0.0034 0.0038 0.0045 0.0094 0.0061 0.025 0.0113 0.0024 0.0014 0.0071 0.0053 0.0076 0.0193 0.0035
Bud Site 0.0051 0.0237 0.0127 0.0043 0.0088 0.0014 0.0062 0.0374 0.0208 0.001 0.0079 0.0013 0.0029 0.03 0.0089 0.0011 0.0077 0.0028
Cell Periphery 0.0009 0.0011 0.0023 0.0008 0.0004 0.0006 0.0024 0.003 0.0107 0.0004 0.0061 0.0016 0.0018 0.0029 0.0029 0.0013 0.0014 0.0024
Cytoplasm 0.0222 0.0469 0.0226 0.01 0.0099 0.0224 0.0395 0.0449 0.0332 0.0026 0.0796 0.0322 0.0241 0.0394 0.0488 0.0473 0.0155 0.0254
Cytoplasmic Foci 0.1262 0.1178 0.106 0.0553 0.1462 0.1022 0.1147 0.0955 0.0908 0.0782 0.0914 0.1144 0.104 0.0877 0.0758 0.053 0.0915 0.1025
Eisosomes 0.0015 0.0007 0.001 0.0005 0.0002 0.0011 0.0015 0.0018 0.0029 0.0002 0.0012 0.0011 0.0011 0.0017 0.0019 0.001 0.0022 0.0012
Endoplasmic Reticulum 0.0038 0.0037 0.0021 0.0016 0.001 0.0026 0.0054 0.0025 0.0041 0.0003 0.0076 0.0016 0.003 0.0038 0.0016 0.0038 0.0028 0.0033
Endosome 0.1287 0.1051 0.1074 0.0837 0.1496 0.1111 0.1668 0.1022 0.114 0.0464 0.1865 0.0875 0.1079 0.1164 0.055 0.0744 0.0903 0.0812
Golgi 0.0827 0.058 0.0589 0.0264 0.1343 0.0712 0.0975 0.082 0.0829 0.0546 0.0992 0.0667 0.0595 0.0606 0.0394 0.0475 0.0714 0.0629
Lipid Particles 0.0565 0.0286 0.025 0.0153 0.0756 0.0388 0.0408 0.011 0.0171 0.031 0.0123 0.0426 0.0565 0.0204 0.0248 0.0128 0.0399 0.023
Mitochondria 0.3712 0.4341 0.4408 0.6453 0.3963 0.4978 0.3685 0.442 0.4217 0.5714 0.3304 0.4734 0.4352 0.452 0.5565 0.5502 0.4944 0.4985
None 0.0448 0.0809 0.0792 0.0822 0.0042 0.0557 0.0479 0.0603 0.088 0.0043 0.0586 0.0556 0.0704 0.0747 0.0681 0.1088 0.0184 0.0589
Nuclear Periphery 0.0023 0.0014 0.0016 0.001 0.0003 0.0015 0.0083 0.0012 0.0016 0.0001 0.0046 0.0003 0.0079 0.0009 0.0007 0.0013 0.0051 0.0008
Nucleolus 0.0019 0.0047 0.0017 0.0013 0.0008 0.0012 0.0008 0.0011 0.0008 0.0003 0.0005 0.0005 0.001 0.001 0.0008 0.0008 0.0005 0.0007
Nucleus 0.0019 0.0034 0.0014 0.0026 0.0004 0.0016 0.002 0.0012 0.001 0.0003 0.0027 0.0005 0.0022 0.0012 0.0008 0.0012 0.0028 0.0008
Peroxisomes 0.0752 0.0564 0.0843 0.0423 0.0495 0.0543 0.0396 0.0514 0.0476 0.1681 0.027 0.0859 0.0813 0.0621 0.0675 0.0664 0.0717 0.0849
Punctate Nuclear 0.005 0.0028 0.0037 0.0007 0.0007 0.0021 0.0034 0.0044 0.0015 0.0003 0.0022 0.0006 0.0097 0.0004 0.003 0.0004 0.0031 0.0036
Vacuole 0.007 0.0122 0.0065 0.0073 0.0039 0.0085 0.0089 0.005 0.0058 0.0028 0.0071 0.0042 0.0054 0.0105 0.0048 0.0109 0.0038 0.0059
Vacuole Periphery 0.0038 0.0041 0.0046 0.0041 0.0027 0.0046 0.0049 0.0022 0.0033 0.0027 0.0042 0.0028 0.0026 0.0036 0.002 0.0063 0.0023 0.0033

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 15.8659 15.4726 17.222 16.781 19.6359 15.8572 14.6665 8.3591 23.1948 18.5573
Translational Efficiency 0.2119 0.303 0.3652 0.5995 0.3267 0.3389 0.4322 1.2115 0.3231 0.3374

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1427 821 391 1715 1924 2405 1947 2184 3351 3226 2338 3899

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 627.95 733.84 834.30 790.64 688.92 820.23 806.07 782.28 662.96 798.24 810.79 785.96
Standard Deviation 68.17 83.51 91.35 124.93 79.84 95.34 97.05 108.13 80.92 99.84 96.70 115.89
Intensity Change Log 2 — 0.224816 0.409917 0.332371 — 0.251692 0.226569 0.183349 — 0.238939 0.316908 0.256332

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000584 0.002261 0.004719 0.004583 0.000479 0.001077 0.002379 0.004954 0.000524 0.001378 0.002770 0.004791
Bud Neck 0.036990 0.025576 0.027394 0.036280 0.021932 0.032316 0.040048 0.049091 0.028344 0.030600 0.037932 0.043456
Bud Site 0.011433 0.029965 0.041877 0.061460 0.010880 0.016551 0.037154 0.105826* 0.011115 0.019965 0.037944 0.086311
Cell Periphery 0.000522 0.000357 0.000275 0.000197 0.000371 0.000214 0.000155 0.000367 0.000435 0.000251 0.000175 0.000292
Cytoplasm 0.084690 0.033498 0.112179* 0.243611* 0.053598 0.033804 0.146389* 0.194431* 0.066838 0.033726 0.140668* 0.216063*
Cytoplasmic Foci 0.291319* 0.187075* 0.154278 0.134231 0.247180* 0.171552 0.176254 0.148600 0.265976* 0.175503 0.172579 0.142280
Eisosomes 0.000534 0.000217 0.000219 0.000136 0.000214 0.000209 0.000128 0.000148 0.000350 0.000211 0.000143 0.000142
Endoplasmic Reticulum 0.002055 0.000885 0.008900 0.003083 0.002315 0.000499 0.004662 0.002096 0.002204 0.000597 0.005371 0.002530
Endosome 0.042852 0.107855 0.061269 0.041338 0.073990 0.091848 0.057522 0.047286 0.060730 0.095922 0.058149 0.044670
Golgi 0.065462 0.144476* 0.014632 0.018011 0.113623* 0.117783* 0.023796 0.024956 0.093114 0.124576* 0.022264 0.021901
Lipid Particles 0.035166 0.008079 0.021100 0.005920 0.021520 0.014745 0.009958 0.005809 0.027331 0.013049 0.011821 0.005858
Mitochondria 0.318314* 0.292348* 0.030975 0.027908 0.340522* 0.397071* 0.065561 0.080726 0.331065* 0.370420* 0.059777 0.057494
Mitotic Spindle 0.001415 0.014437 0.077021 0.074754 0.006530 0.010343 0.101623* 0.054410 0.004352 0.011385 0.097508 0.063359
None 0.001644 0.000529 0.003554 0.002357 0.001684 0.001121 0.002870 0.002077 0.001667 0.000970 0.002984 0.002200
Nuclear Periphery 0.000327 0.000231 0.006072 0.002174 0.000321 0.000169 0.002912 0.000792 0.000323 0.000185 0.003441 0.001400
Nuclear Periphery Foci 0.000165 0.000315 0.005272 0.001711 0.000323 0.000187 0.001869 0.000579 0.000256 0.000220 0.002438 0.001077
Nucleolus 0.001548 0.002109 0.014987 0.002283 0.001659 0.001285 0.007933 0.002681 0.001612 0.001495 0.009113 0.002506
Nucleus 0.019275 0.008536 0.207419* 0.090269 0.012074 0.006719 0.110820 0.051348 0.015140 0.007181 0.126975 0.068468
Peroxisomes 0.056662 0.105768* 0.076840 0.050875 0.039513 0.062237 0.068953 0.090305 0.046815 0.073315 0.070272 0.072961
Vacuole 0.018859 0.023535 0.123907 0.191221 0.033659 0.030301 0.130709 0.128214 0.027357 0.028579 0.129572 0.155928
Vacuole Periphery 0.010185 0.011946 0.007112 0.007598 0.017614 0.009969 0.008305 0.005305 0.014451 0.010472 0.008106 0.006314

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -4.47 -6.68 -5.82 -2.27 1.38 -4.26 -5.19 -9.93 -8.20 -4.44 -5.97 -7.07 -11.16 -8.07 -3.44
Bud Neck 3.03 2.76 1.16 -2.43 -2.10 -4.23 -8.04 -10.41 -5.70 -2.86 -1.06 -4.76 -6.63 -5.24 -1.62
Bud Site -6.80 -5.87 -12.59 -5.12 -1.66 -4.68 -13.50 -22.20 -20.40 -14.80 -7.70 -14.62 -25.25 -20.84 -13.43
Cell Periphery 2.34 3.72 5.88 4.76 4.83 1.97 2.82 0.30 -1.17 -1.84 3.45 5.05 2.30 -0.15 -1.56
Cytoplasm 10.37 -3.28 -22.45 -30.69 -13.01 6.32 -18.22 -25.33 -30.55 -7.27 12.29 -16.70 -33.80 -43.79 -13.40
Cytoplasmic Foci 12.38 15.09 25.47 10.21 3.86 13.03 13.09 18.06 5.80 4.97 18.98 20.04 30.37 11.26 7.98
Eisosomes 7.59 7.39 10.42 5.26 6.59 0.35 6.81 5.08 5.58 -2.17 7.06 11.08 11.48 7.95 1.53
Endoplasmic Reticulum 2.90 -8.07 -3.20 -8.01 7.03 5.05 -5.62 0.24 -11.40 8.09 6.19 -9.13 -1.82 -14.95 9.48
Endosome -11.85 -2.57 3.78 14.08 4.55 -4.80 6.22 9.66 14.56 3.60 -11.83 2.53 10.19 20.85 6.70
Golgi -11.19 13.82 15.56 20.27 -0.05 -0.87 23.75 23.31 26.62 -0.56 -8.09 26.58 27.75 34.10 1.02
Lipid Particles 12.16 5.48 15.39 2.15 8.26 3.88 8.23 11.54 7.17 6.03 9.83 12.60 19.00 7.61 9.73
Mitochondria 1.94 31.05* 34.47* 24.62* 1.34 -5.88 36.72* 34.01* 43.94* -3.01 -5.03 46.49* 48.21* 52.86* 1.18
Mitotic Spindle -7.24 -8.57 -15.70 -11.53 0.73 -3.36 -19.79* -15.61 -14.07 8.19 -7.74 -21.93 -22.06 -18.50 7.29
None 4.55 -2.29 -1.06 -3.62 1.67 1.44 -1.27 -0.07 -1.36 1.13 2.23 -2.13 -0.62 -2.51 1.56
Nuclear Periphery 0.90 -5.09 -6.51 -6.74 3.01 3.11 -7.69 -6.40 -8.30 5.73 2.96 -9.19 -8.38 -9.38 5.06
Nuclear Periphery Foci -2.30 -6.10 -5.97 -5.35 4.07 3.71 -7.25 -5.09 -7.22 5.55 1.36 -9.59 -7.21 -7.47 5.19
Nucleolus -0.91 -6.03 -2.49 -0.48 5.68 1.70 -8.64 -2.59 -3.84 6.96 0.72 -10.59 -3.52 -3.84 9.22
Nucleus 4.40 -14.79 -20.36 -22.21 7.82 4.10 -24.89 -17.65 -20.10 11.99 6.75 -28.13 -26.70 -30.35 11.40
Peroxisomes -7.54 -2.22 4.32 10.08 4.43 -7.23 -7.31 -11.06 -4.93 -4.13 -9.15 -6.22 -6.33 2.68 0.48
Vacuole -1.99 -13.16 -37.23 -35.15 -10.21 1.36 -25.10 -25.75 -26.80 -0.96 -0.75 -30.01 -43.70 -42.74 -9.42
Vacuole Periphery -1.29 3.98 4.92 4.83 0.74 7.45 10.19 13.89 8.70 4.50 5.19 10.05 13.87 9.10 3.82
Standard name
Human Ortholog
Description Mitochondrial translational activator specific for the COX3 mRNA; acts together with Pet54p and Pet494p; located in the mitochondrial inner membrane
Localization
Cell Percentages mitochondrion (83%)
Cell Cycle Regulation No
Subcompartmental Group mito-1

Pet122

Pet122


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Pet122-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available