Standard name
Human Ortholog
Description Rho GTPase activating protein (RhoGAP); involved in the control of cytoskeleton organization and cellular morphogenesis; required for bud emergence; potential GAP for Rho4p
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.14 0 0.08 0.09 0.14 0.09 0.05 0 0 0 0 0 0.07 0.11 0.09 0.08 0.05 0 0.07 0.06 0.05 0.14 0.11 0.08
Bud 0 0 0.12 0.12 0.1 0.13 0.08 0.09 0.06 0.09 0.07 0.11 0 0 0 0 0 0 0 0 0 0.05 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.1 0.13 0.13 0.18 0.06 0
Bud Site 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0.56 0.45 0.28 0.31 0.17 0.19 0.19 0.13 0.21 0.16 0.22 0.17 0.55 0.58 0.59 0.63 0.42 0.61 0.27 0.17 0.25 0.15 0.23 0.16
Cytoplasm 0.63 0.82 0.82 0.74 0.65 0.67 0.66 0.63 0.6 0.66 0.62 0.58 0.82 0.69 0.74 0.6 0.66 0.67 0.43 0.57 0.42 0.37 0.45 0.61
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0.05 0 0 0.06 0.1 0.07
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0.12 0.1 0.21 0.24 0.31 0.37 0.41 0.42 0.44 0 0 0 0 0 0 0 0 0.05 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0.1 0.09 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 19 6 37 29 64 47 32 27 14 12 10 9 16 31 26 12 6 3 11 10 25 36 34 22
Bud 1 5 59 40 47 71 54 54 23 48 33 57 6 11 8 3 1 1 2 1 5 13 5 4
Bud Neck 0 0 8 1 9 5 4 3 5 2 5 3 0 0 0 0 0 0 17 22 66 45 17 9
Bud Site 0 0 7 18 16 23 11 8 8 6 2 10 0 0 0 0 0 0 – – – – – –
Cell Periphery 78 67 134 104 78 100 124 79 79 86 97 87 133 157 173 89 54 78 46 30 129 37 71 47
Cytoplasm 88 123 388 250 300 363 428 397 225 344 275 289 199 187 214 85 84 85 71 100 218 93 141 175
Endoplasmic Reticulum 6 6 2 0 17 7 14 9 17 3 3 2 1 5 7 3 3 5 8 1 22 14 31 20
Endosome 0 0 1 1 3 0 1 0 0 0 0 1 1 0 0 0 0 1 4 0 1 2 1 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 1 1 1 0 0 0 2 1
Mitochondria 1 2 9 42 47 114 154 197 138 215 188 222 0 1 6 2 1 1 1 2 28 2 3 1
Nucleus 0 0 1 1 2 2 3 8 1 2 3 0 3 1 3 8 5 4 0 0 0 0 0 0
Nuclear Periphery 0 0 0 1 2 0 1 6 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 1 0 1 0 0 1 0 1 3 0 0 0 0 1 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 3 0 9 8 18 11 7 7 4 6 4 6 1 0 1 3 6 2 0 0 0 0 0 0
Vac/Vac Membrane 0 1 3 3 11 7 10 31 4 15 10 15 0 0 0 4 13 11 0 1 11 3 1 1
Unique Cell Count 140 150 472 338 463 538 648 631 377 522 445 500 242 272 291 142 128 127 168 174 518 254 313 289
Labelled Cell Count 196 210 659 498 615 750 843 827 518 740 634 701 360 393 438 213 174 192 168 174 518 254 313 289


Bud Neck, Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 10.3 9.1 5.8 5.4 5.6 5.1 4.8 4.5 4.2 4.3 4.0 3.9 8.5 8.5 7.5 10.1 11.2 11.6 9.6 10.5 11.1
Std Deviation (1e-4) 1.5 1.4 0.9 0.9 0.7 0.9 0.9 0.8 0.8 1.1 0.7 0.9 1.1 1.1 0.9 1.5 2.2 2.4 1.4 1.5 1.6
Intensity Change (Log2) – – – -0.11 -0.06 -0.18 -0.28 -0.36 -0.45 -0.43 -0.52 -0.55 0.55 0.55 0.37 0.8 0.95 1.0 0.73 0.86 0.93


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0.4 2.9 0.5 0 0 0 0 0 0 -0.6 1.6 0.5 0.2 -1.2 -2.2
Bud -0.3 -1.1 0.3 0 0 0 0 0 0 -4.4 -3.8 -4.6 -3.6 -3.9 -3.9
Bud Neck 0 0.3 -1.1 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 3.1 1.9 2.6 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0.7 -4.2 -3.7 0 0 0 0 0 0 6.9 7.9 8.5 7.4 3.0 6.9
Cytoplasm -2.8 -6.0 -5.3 -6.0 -7.0 -7.3 -5.8 -6.9 -8.3 0 -4.2 -2.8 -5.6 -4.1 -3.7
Endoplasmic Reticulum 0 3.5 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 6.1 5.3 9.3 0 0 0 0 0 0 0 0 0.1 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0.5 1.8 0.2 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 2.2 0 1.4 4.1 0 2.6 2.1 2.7 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.3164 5.9958 5.0012 5.0337 5.7005 5.7787 6.9278 8.5661 7.6399 7.6476 7.7155 7.9534 8.8443 9.7448 9.3943 8.7344 9.2925 9.7769
Actin 0.065 0.0094 0.0435 0.0296 0.0119 0.0093 0.0173 0.0185 0.0467 0.057 0.0024 0.0054 0.0628 0.0223 0.0354 0.0236 0.0624 0.0138
Bud 0.0009 0.0006 0.0051 0.0013 0.0008 0.0009 0.0007 0.0037 0.0076 0.0016 0.0006 0.0015 0.0032 0.0049 0.0052 0.002 0.0026 0.0035
Bud Neck 0.0128 0.0053 0.051 0.0441 0.2243 0.2494 0.0144 0.0122 0.0548 0.0887 0.2458 0.2084 0.021 0.009 0.0355 0.1177 0.239 0.2251
Bud Periphery 0.0026 0.001 0.0141 0.0114 0.0054 0.0044 0.0031 0.004 0.0219 0.0039 0.0042 0.0053 0.0102 0.0071 0.0216 0.0135 0.0199 0.0128
Bud Site 0.0238 0.1007 0.0558 0.0023 0.0023 0.0033 0.0532 0.247 0.1039 0.0137 0.008 0.0032 0.0855 0.2104 0.1079 0.0097 0.009 0.0039
Cell Periphery 0.0975 0.0519 0.0874 0.1495 0.1038 0.0456 0.1277 0.0742 0.1369 0.1432 0.1393 0.085 0.2272 0.0962 0.1791 0.2102 0.1117 0.1088
Cytoplasm 0.639 0.7888 0.6402 0.7461 0.6338 0.6609 0.6028 0.5976 0.5489 0.624 0.5306 0.6755 0.5287 0.6293 0.5942 0.5182 0.477 0.6119
Cytoplasmic Foci 0.0112 0.0029 0.0149 0.002 0.0011 0.0036 0.0138 0.003 0.0209 0.0076 0.0027 0.0011 0.0138 0.0034 0.0018 0.0152 0.0163 0.0027
Eisosomes 0.0022 0.0004 0.0007 0.0002 0.0001 0.0002 0.0024 0.0002 0.0004 0.0003 0 0.0001 0.0007 0.0005 0.0005 0.0003 0.0007 0.0003
Endoplasmic Reticulum 0.0234 0.0083 0.0049 0.0035 0.01 0.0066 0.018 0.0085 0.0042 0.0121 0.0016 0.0045 0.0061 0.0026 0.0042 0.0043 0.0026 0.0013
Endosome 0.0092 0.0008 0.0102 0.0002 0.0002 0.0018 0.0053 0.006 0.0135 0.0105 0.0493 0.0002 0.0056 0.001 0.0007 0.0261 0.0145 0.0037
Golgi 0.0145 0.001 0.0137 0.0009 0.0005 0.0027 0.0035 0.0011 0.0086 0.0082 0.0033 0.0004 0.0078 0.0022 0.0015 0.0246 0.02 0.0041
Lipid Particles 0.0118 0.0033 0.0125 0.0015 0.0015 0.0026 0.0101 0.0027 0.0058 0.0034 0.0007 0.0006 0.0047 0.0015 0.0006 0.0122 0.0018 0.0003
Mitochondria 0.0167 0 0.0058 0 0 0.0026 0.0162 0.0001 0.0009 0.0032 0.0014 0 0.0054 0.0004 0.0071 0.0119 0.0149 0.0023
None 0.0279 0.0224 0.0219 0.0035 0.0005 0.0009 0.0795 0.0125 0.0142 0.0022 0.0003 0.0068 0.0024 0.0012 0.0013 0.0011 0.0007 0.0033
Nuclear Periphery 0.0039 0.0004 0.0011 0.0002 0.0003 0.0002 0.0062 0.0006 0.0006 0.0018 0.003 0.0001 0.0005 0.0003 0.0002 0.0004 0.0002 0.0001
Nucleolus 0.0005 0.0002 0.0006 0.0001 0.0001 0.0001 0.0001 0.0002 0.0003 0.0002 0.0001 0.0001 0.0002 0.0003 0.0001 0.0003 0.0001 0.0001
Nucleus 0.0102 0.0005 0.0009 0.0004 0.0004 0.0005 0.0023 0.0009 0.0009 0.0012 0.0006 0.0005 0.0008 0.0008 0.0005 0.0007 0.0004 0.0004
Peroxisomes 0.0084 0.0001 0.0094 0.0001 0 0.003 0.0134 0.0001 0.0034 0.0125 0.0002 0 0.0053 0.0012 0.0002 0.0038 0.0044 0.0002
Punctate Nuclear 0.0145 0.0003 0.0017 0.0001 0.0001 0.0002 0.0028 0.0003 0.0013 0.001 0.0003 0.0001 0.0003 0.0001 0.0001 0.0005 0.0003 0.0001
Vacuole 0.0036 0.0016 0.0039 0.0029 0.0028 0.0013 0.0066 0.0065 0.0039 0.0035 0.0029 0.0013 0.0075 0.0052 0.0023 0.0033 0.0014 0.0013
Vacuole Periphery 0.0005 0 0.0004 0 0 0.0001 0.0004 0.0002 0.0003 0.0003 0.0026 0 0.0002 0.0001 0.0001 0.0006 0.0002 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 49.1137 35.9427 40.8505 61.0882 47.5111 38.1501 51.7523 54.438 45.1363 71.5783
Translational Efficiency 0.4893 0.509 0.4277 0.2943 0.4043 0.5754 0.3938 0.2806 0.4137 0.3528

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Rho GTPase activating protein (RhoGAP); involved in the control of cytoskeleton organization and cellular morphogenesis; required for bud emergence; potential GAP for Rho4p
Localization
Cell Percentages cytoplasm (75%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-10

Bem2

Bem2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Bem2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available