Standard name
Human Ortholog
Description 5' to 3' DNA helicase; involved in nucleotide excision repair and transcription; subunit of RNA polII initiation factor TFIIH and of Nucleotide Excision Repair Factor 3 (NEF3); homolog of human XPD protein; mutant has aneuploidy tolerance; protein abundance increases in response to DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0.06 0 0 0 0.06 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.11 0.15 0.07 0.08 0.1 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0.05 0.06 0 0.2 0.28 0.17 0.56 0.48 0.6 0.34 0.13 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.91 0.91 0.97 0.97 0.96 0.88 0.85 0.88 0.79 0.78 0.84 0.89 0.86 0.87 0.81 0.88 0.87 0.86 0.83 0.86 0.84 0.72 0.63 0.58
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.15 0.08 0 0 0 0 0.06 0.06 0.1 0.08 0.08 0.09 0.09 0.08 0.09 0 0 0 0.09 0.05 0.06 0.11 0.18 0.17
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.11 0.13
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0
Bud 0 0 0 0 0 1 2 3 9 3 2 3 0 0 0 0 1 0 4 2 0 1 1 5
Bud Neck 0 0 0 0 0 1 2 2 0 2 1 2 0 0 0 0 0 0 0 0 0 0 2 0
Bud Site 1 1 0 0 1 1 15 4 12 11 12 3 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 5 6 2 2 5 5 7 8 9 11 6 3 11 22 35 25 24 32 1 0 0 0 0 0
Endoplasmic Reticulum 0 1 0 0 0 0 0 0 0 1 0 0 2 2 0 3 5 8 0 0 0 0 3 3
Endosome 0 3 0 1 0 0 0 0 0 0 0 0 0 1 0 5 4 3 5 4 1 0 1 4
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 2 1 0 1 0 0
Mitochondria 13 9 8 9 4 33 73 45 176 134 112 70 23 1 9 10 5 14 5 2 2 1 1 3
Nucleus 426 288 144 139 195 146 221 229 249 219 157 185 156 180 189 330 265 283 416 287 131 99 156 132
Nuclear Periphery 2 1 1 0 0 0 1 1 4 2 0 0 0 1 4 2 3 1 0 0 0 0 0 1
Nucleolus 69 24 5 3 5 5 15 15 33 23 15 19 16 16 20 5 9 13 44 16 9 15 44 39
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 1 0 0 0 0 0 0 0
SpindlePole 1 1 2 4 2 2 10 5 6 1 3 2 2 1 3 0 2 2 3 0 0 2 4 2
Vac/Vac Membrane 12 9 0 1 1 1 1 1 1 2 2 0 1 2 2 11 10 7 12 13 5 11 26 30
Unique Cell Count 470 316 148 143 203 165 259 259 315 280 188 208 182 207 232 376 306 329 500 332 157 139 249 230
Labelled Cell Count 529 344 162 159 214 195 347 313 499 409 310 287 211 227 262 392 330 363 500 332 157 139 249 230


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.1 7.0 5.8 5.5 6.1 4.6 4.8 5.0 4.1 4.1 3.8 4.3 6.4 6.9 6.6 8.6 9.1 8.6 6.4 6.5 6.9
Std Deviation (1e-4) 1.0 1.1 0.7 0.9 1.2 0.8 1.0 0.7 0.8 0.8 1.0 0.8 1.3 1.7 1.6 1.7 1.8 1.9 1.7 1.3 1.7
Intensity Change (Log2) – – – -0.06 0.09 -0.31 -0.25 -0.21 -0.5 -0.5 -0.59 -0.41 0.14 0.25 0.2 0.58 0.66 0.58 0.15 0.17 0.26


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 2.2 3.4 4.4 2.5 2.8 3.3
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.3 -1.7 0 0 3.5 0 0 0 0 2.2 0 -0.7 -1.6 0 -0.6
Nucleus 0 -0.6 -3.0 -3.8 -3.1 -5.1 -5.2 -4.1 -2.9 -3.6 -3.4 -4.6 -3.3 -3.6 -3.7
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 1.1 0 0 0 0 2.0 1.7 2.0 0 0 0.3
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.1436 1.2154 1.1855 1.2404 0.7858 0.9459 2.847 3.178 2.9458 2.2874 2.2087 2.6586 2.3198 2.3793 2.2356 2.1534 1.8834 2.1207
Actin 0.0585 0 0.0084 0.0204 0.011 0.0105 0.0251 0 0.0017 0.0012 0.0064 0.001 0.0141 0 0.0074 0.0066 0.0049 0.0012
Bud 0.001 0 0.0001 0.0066 0.001 0.0001 0.0005 0 0.0006 0.0005 0.0025 0 0.0004 0 0 0.0001 0.0003 0.0001
Bud Neck 0.0078 0.0002 0.0001 0.0009 0.0018 0.0013 0.001 0.0001 0.0005 0.0004 0.007 0.0006 0.0008 0 0.0002 0.0002 0.001 0.0011
Bud Periphery 0.002 0 0.0001 0.0093 0.0039 0.0003 0.001 0 0.0005 0.0037 0.0083 0.0001 0.0008 0 0.0001 0.0003 0.0004 0.0003
Bud Site 0.0204 0.0001 0.0001 0.0344 0.004 0.0004 0.0019 0 0.0014 0.0006 0.004 0.0001 0.0019 0 0.0004 0.0005 0.0016 0.0003
Cell Periphery 0.001 0 0 0.0005 0.0008 0.0001 0.0002 0 0.0001 0.0008 0.0008 0 0.0006 0 0.0001 0.0001 0 0.0001
Cytoplasm 0.0133 0.0004 0.0002 0.0003 0.0007 0.0003 0.0032 0 0.0001 0.0011 0.0024 0.0001 0.0038 0 0.0001 0.0094 0.0008 0.0007
Cytoplasmic Foci 0.023 0.0032 0.0015 0.0013 0.0092 0.0016 0.0134 0 0.0017 0.0012 0.0049 0.0007 0.0104 0 0.0003 0.0043 0.0247 0.0014
Eisosomes 0.0005 0 0 0.0001 0.0003 0.0001 0.0002 0 0 0.0001 0.0001 0 0.0003 0 0 0.0002 0 0
Endoplasmic Reticulum 0.0052 0.0001 0.0005 0.0002 0.0013 0.0008 0.0037 0 0.0001 0.005 0.0029 0.0001 0.0028 0 0.0001 0.0087 0.0002 0.0009
Endosome 0.0342 0.0034 0.0093 0.0011 0.0215 0.006 0.0175 0 0.0013 0.0102 0.0259 0.0013 0.0139 0 0.0015 0.0313 0.009 0.0072
Golgi 0.0103 0.0002 0.0029 0.0017 0.0105 0.0052 0.0063 0 0.0022 0.001 0.0053 0.0017 0.006 0 0.0003 0.0033 0.0077 0.0017
Lipid Particles 0.0153 0.0008 0.002 0.0008 0.0386 0.0046 0.0065 0 0.0018 0.0056 0.0095 0.0014 0.0183 0 0.0018 0.013 0.0157 0.0047
Mitochondria 0.0223 0.0002 0.0006 0.0028 0.0229 0.0214 0.0023 0.0001 0.0079 0.0245 0.016 0.007 0.015 0 0.0013 0.0108 0.0035 0.0015
None 0.0047 0 0.0002 0.0001 0.0011 0.0003 0.0013 0 0.0001 0.0007 0.0007 0 0.0041 0 0.0001 0.0027 0.0009 0.0016
Nuclear Periphery 0.0266 0.0009 0.001 0.0004 0.0122 0.0031 0.0144 0.0004 0.0011 0.0357 0.0125 0.0021 0.0133 0.0001 0.0021 0.049 0.0033 0.0161
Nucleolus 0.0216 0.0242 0.0089 0.0133 0.0704 0.0472 0.0273 0.0165 0.013 0.0076 0.0527 0.049 0.0235 0.0117 0.0117 0.0129 0.0564 0.0613
Nucleus 0.695 0.9624 0.9629 0.9033 0.7586 0.8931 0.855 0.9817 0.9623 0.8859 0.7948 0.9314 0.8435 0.9876 0.9689 0.831 0.8305 0.8923
Peroxisomes 0.0079 0.0001 0.0005 0.0009 0.0065 0.0015 0.0097 0 0.0014 0.0003 0.0028 0.0005 0.0067 0 0.0002 0.0006 0.0164 0.0006
Punctate Nuclear 0.0194 0.0034 0.0005 0.0005 0.0181 0.0013 0.0049 0.001 0.0003 0.0027 0.0106 0.0026 0.0142 0.0005 0.001 0.0082 0.0221 0.0049
Vacuole 0.0063 0.0002 0.0002 0.0009 0.0035 0.0004 0.003 0.0001 0.0016 0.0061 0.0251 0.0001 0.0034 0 0.0016 0.0038 0.0003 0.0009
Vacuole Periphery 0.0037 0 0.0002 0.0002 0.002 0.0006 0.0015 0 0.0004 0.0051 0.0048 0.0001 0.0022 0 0.0009 0.003 0.0002 0.001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 21.7187 21.1035 21.9236 21.4636 20.5073 15.1003 20.5909 23.1864 20.8234 21.5121
Translational Efficiency 0.6659 0.6033 0.5813 0.5973 0.5837 0.8208 0.6201 0.3827 0.5466 0.5842

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1788 1184 82 1279 1893 1341 777 2023 3681 2525 859 3302

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 751.78 898.12 1207.32 1016.16 785.29 871.60 1017.45 1260.47 769.01 884.04 1035.57 1165.84
Standard Deviation 100.65 141.30 176.17 153.44 112.19 124.04 138.61 228.36 108.05 133.07 153.15 235.02
Intensity Change Log 2 — 0.256598 0.683426 0.434745 — 0.150441 0.373660 0.682664 — 0.203338 0.533473 0.566717

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000254 0.000590 0.001934 0.001208 0.000301 0.000446 0.001213 0.000287 0.000279 0.000514 0.001282 0.000643
Bud Neck 0.004522 0.014864 0.011706 0.038571 0.006546 0.009297 0.015849 0.035277 0.005563 0.011907 0.015453 0.036553
Bud Site 0.000486 0.001698 0.001626 0.004254 0.000492 0.000806 0.001456 0.002028 0.000489 0.001224 0.001472 0.002890
Cell Periphery 0.000062 0.000077 0.000043 0.000119 0.000064 0.000073 0.000062 0.000113 0.000063 0.000075 0.000061 0.000115
Cytoplasm 0.002429 0.002076 0.000170 0.003046 0.001078 0.000409 0.000801 0.001169 0.001734 0.001191 0.000741 0.001896
Cytoplasmic Foci 0.000117 0.000172 0.000051 0.003118 0.000378 0.001051 0.000021 0.000013 0.000252 0.000639 0.000024 0.001216
Eisosomes 0.000017 0.000031 0.000083 0.000037 0.000020 0.000027 0.000048 0.000037 0.000018 0.000029 0.000052 0.000037
Endoplasmic Reticulum 0.001574 0.004331 0.003847 0.003958 0.002628 0.003720 0.003686 0.001486 0.002116 0.004007 0.003701 0.002444
Endosome 0.000140 0.000654 0.000105 0.005133 0.000176 0.000609 0.000134 0.000080 0.000159 0.000630 0.000131 0.002037
Golgi 0.000094 0.000213 0.000185 0.006401 0.000101 0.000186 0.000122 0.000046 0.000098 0.000199 0.000128 0.002508
Lipid Particles 0.000119 0.000191 0.000118 0.000646 0.000262 0.000177 0.000071 0.000005 0.000193 0.000184 0.000075 0.000253
Mitochondria 0.000552 0.001515 0.001833 0.003462 0.001367 0.002506 0.001420 0.000938 0.000972 0.002041 0.001460 0.001916
Mitotic Spindle 0.000336 0.002767 0.000312 0.038474 0.001000 0.001683 0.000959 0.000919 0.000678 0.002191 0.000897 0.015466
None 0.005876 0.002432 0.000855 0.005984 0.003210 0.001747 0.002873 0.001378 0.004505 0.002068 0.002680 0.003162
Nuclear Periphery 0.000191 0.000329 0.000161 0.002098 0.000282 0.000516 0.000268 0.000291 0.000238 0.000428 0.000258 0.000991
Nuclear Periphery Foci 0.000196 0.000592 0.000110 0.002179 0.000251 0.000487 0.000132 0.000011 0.000224 0.000536 0.000130 0.000851
Nucleolus 0.056918 0.042515 0.053952 0.020534 0.039001 0.036959 0.036475 0.006734 0.047704 0.039565 0.038143 0.012079
Nucleus 0.925513* 0.921122* 0.918888* 0.851808* 0.941903* 0.937573* 0.932706* 0.948649* 0.933942* 0.929859* 0.931387* 0.911139*
Peroxisomes 0.000296 0.000400 0.002455 0.000504 0.000495 0.000454 0.000678 0.000027 0.000398 0.000429 0.000847 0.000212
Vacuole 0.000210 0.001797 0.001354 0.005420 0.000317 0.000744 0.000889 0.000394 0.000265 0.001238 0.000934 0.002341
Vacuole Periphery 0.000097 0.001632 0.000213 0.003046 0.000127 0.000530 0.000138 0.000117 0.000112 0.001047 0.000145 0.001252

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -8.02 -12.51 -24.15 -15.57 2.93 -3.39 -19.13 -1.42 2.83 19.64* -7.90 -22.89 -19.67 -9.46 11.41
Bud Neck -8.65 -6.26 -19.32 -12.58 -12.46 -3.17 -9.24 -21.95 -18.64 -10.40 -8.74 -10.62 -29.42 -21.77 -13.24
Bud Site -3.07 -7.35 -8.10 -5.12 -5.10 -2.08 -9.84 -3.16 -2.56 -0.32 -3.64 -11.06 -7.06 -4.86 -3.06
Cell Periphery -0.96 1.08 -4.35 -3.01 -7.95 -0.24 -0.05 -1.12 -0.55 -1.15 -0.59 -0.06 -2.34 -1.23 -2.40
Cytoplasm 0.39 3.16 -1.06 -1.28 -6.59 2.56 0.85 -1.25 -5.14 -2.76 1.16 2.76 -1.09 -2.31 -6.25
Cytoplasmic Foci -0.29 1.81 -2.43 -2.37 -2.66 -1.19 1.88 1.96 1.77 2.03 -1.22 2.37 -0.81 0.83 -2.67
Eisosomes -8.44 -9.98 -19.29 -8.09 5.77 -4.73 -17.83 -4.23 -1.93 5.86 -9.29 -21.44 -9.84 -4.53 7.12
Endoplasmic Reticulum -11.30 -6.07 -13.91 -1.69 -1.97 -2.59 -6.62 8.07 5.42 12.83 -7.49 -10.27 -5.76 4.57 6.55
Endosome -2.17 0.51 -3.32 -2.29 -3.35 -2.04 -0.40 3.73 2.51 5.16 -2.98 -1.00 -3.06 -0.85 -2.96
Golgi -1.88 -4.45 -2.58 -2.45 -2.44 -1.09 -2.21 1.84 1.84 10.83 -2.02 -3.93 -2.50 -2.22 -2.31
Lipid Particles -0.57 0.10 -1.70 -1.22 -1.87 0.68 1.38 2.05 2.32 9.87 0.31 1.52 0.74 0.51 -0.96
Mitochondria -3.17 -9.79 -14.03 -6.63 -7.24 -1.17 -0.50 0.42 1.74 3.93 -2.03 -2.38 -4.22 -0.55 -3.90
Mitotic Spindle -2.05 -0.47 -7.92 -6.92 -7.90 -1.20 -1.71 -0.90 0.34 0.86 -2.36 -2.30 -7.75 -6.15 -6.08
None 4.49 6.86 0.81 -3.21 -5.28 2.27 0.89 2.29 -0.34 2.19 4.89 3.81 2.60 -2.75 -1.48
Nuclear Periphery -2.64 -0.47 -8.29 -7.45 -8.20 -1.89 -2.23 -2.18 1.10 0.29 -2.75 -3.65 -8.62 -5.19 -6.75
Nuclear Periphery Foci -1.12 -0.19 -2.66 -0.93 -2.15 -1.05 1.26 3.10 2.63 4.63 -1.53 1.25 -1.10 0.73 -1.82
Nucleolus 5.02 -0.36 11.17 5.98 3.51 0.91 0.05 14.98 13.88 10.18 4.47 2.34 19.18 14.50 9.00
Nucleus 0.67 1.61 10.09 8.93 3.84 1.15 4.52 1.19 -0.17 -3.67 1.19 3.29 7.83 6.02 3.09
Peroxisomes -0.61 -6.13 -1.75 -2.34 5.82 0.08 -1.12 2.85 5.32 13.98 -0.23 -4.19 2.00 4.39 11.12
Vacuole -3.08 -4.66 -6.81 -3.73 -4.07 -1.27 -3.71 -0.41 1.25 4.97 -3.30 -5.95 -6.38 -2.61 -2.69
Vacuole Periphery -3.57 -7.43 -2.65 0.58 -2.30 -2.47 -2.12 0.75 2.56 2.70 -4.26 -5.01 -2.60 1.73 -2.27
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Components••••••••••••••••actinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
Standard name
Human Ortholog
Description 5' to 3' DNA helicase; involved in nucleotide excision repair and transcription; subunit of RNA polII initiation factor TFIIH and of Nucleotide Excision Repair Factor 3 (NEF3); homolog of human XPD protein; mutant has aneuploidy tolerance; protein abundance increases in response to DNA replication stress
Localization
Cell Percentages nucleus (98%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Rad3

Rad3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rad3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available