Standard name
Human Ortholog
Description Checkpoint protein; involved in the activation of the DNA damage and meiotic pachytene checkpoints; subunit of a clamp loader that loads Rad17p-Mec3p-Ddc1p onto DNA; homolog of human and S. pombe Rad17 protein
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0.06 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.43 0.38 0.18 0.59 0.62 0.63 0.55 – 0.46 0.42 0.32 0.41 0.61 0.73 0.77 0.49 0.47 0.56 0.23 0.22 0.3 0.45 0.49 0.51
Endoplasmic Reticulum 0 0 0.18 0 0 0 0 – 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.23 0 0 0.08 0 0.15 0.25 – 0.45 0.39 0.7 0.46 0 0 0 0 0 0 0 0 0.07 0 0 0
Nucleus 0.17 0.32 0.82 0.23 0.16 0.16 0.2 – 0.18 0.2 0.15 0.24 0.32 0.2 0.09 0.12 0.12 0.11 0.38 0.45 0.17 0.2 0.16 0.17
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.39 0.44 0 0.12 0.26 0.16 0.14 – 0.09 0.14 0 0 0.12 0.17 0.15 0.47 0.45 0.38 0.28 0.25 0.36 0.27 0.21 0.2
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 1
Bud 0 0 1 0 0 1 3 – 1 2 5 0 0 0 0 0 0 0 1 1 0 1 2 2
Bud Neck 0 0 0 1 1 1 1 – 0 4 4 1 4 0 2 0 0 0 1 0 0 0 2 4
Bud Site 0 0 0 0 0 0 0 – 1 0 1 2 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 1 0 0 2 0 2 1 0 0 0 0 0 0 0
Cytoplasm 53 70 3 39 69 97 142 – 81 96 64 91 75 140 184 121 135 125 38 45 21 27 103 112
Endoplasmic Reticulum 1 0 3 0 1 0 0 – 0 1 1 2 0 1 0 11 14 6 0 0 0 0 0 0
Endosome 0 3 0 0 0 1 3 – 1 1 0 2 0 3 7 11 12 6 1 1 0 0 2 0
Golgi 0 0 0 0 1 0 0 – 0 0 0 0 0 1 0 1 4 0 0 0 0 0 0 0
Mitochondria 28 8 0 5 3 23 65 – 80 90 142 101 0 1 0 4 8 8 6 4 5 0 4 4
Nucleus 21 59 14 15 18 25 53 – 32 46 31 52 39 38 21 30 33 25 63 92 12 11 34 38
Nuclear Periphery 0 0 0 0 0 0 0 – 0 1 3 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0
SpindlePole 0 0 0 2 0 0 2 – 0 4 2 0 0 0 0 0 0 0 3 3 2 1 7 5
Vac/Vac Membrane 47 81 0 8 29 25 36 – 16 32 9 9 15 32 36 117 128 85 47 51 25 16 45 44
Unique Cell Count 122 186 17 66 112 155 260 177 229 203 221 123 193 240 247 285 223 167 206 72 60 211 220
Labelled Cell Count 150 221 21 70 122 173 305 212 277 263 260 133 218 251 298 335 256 167 206 72 60 211 220


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.8 4.6 4.6 5.0 5.8 4.6 4.4 – 3.9 4.0 3.4 3.8 5.2 5.3 5.2 6.2 6.1 5.8 – 5.0 5.1
Std Deviation (1e-4) 0.5 1.0 0.3 1.1 2.4 1.3 1.4 – 1.1 1.2 0.8 1.1 0.9 1.0 1.2 1.5 1.5 1.5 – 1.1 1.0
Intensity Change (Log2) – – – 0.13 0.35 -0.0 -0.04 – -0.24 -0.17 -0.42 -0.27 0.2 0.23 0.19 0.45 0.42 0.36 – 0.12 0.17


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 3.8 3.6 3.2

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -1.0326 -0.7805 -0.8863 -0.7216 -0.6715 -1.1075 -0.5573 -0.0132 -0.2533 -0.7371 -0.4179 -0.3997 -1.6065 -1.3105 -1.5157 -1.1021 -1.3836 -1.4694
Actin 0.0215 0.0012 0.0129 0.0046 0.0128 0.0057 0.0374 0.0003 0.0031 0.0248 0.0116 0.0117 0.004 0.0003 0.003 0.01 0.0207 0.0014
Bud 0.0007 0.0023 0.0026 0.0008 0.0005 0.0001 0.0006 0.0002 0.0006 0.0048 0.0014 0.0002 0.0008 0.0001 0.0001 0.0014 0.0025 0.0001
Bud Neck 0.0016 0.0006 0.0012 0.0006 0.002 0.0049 0.0122 0.0004 0.0005 0.0021 0.0028 0.0092 0.0084 0.0003 0.0006 0.0035 0.001 0.0021
Bud Periphery 0.0013 0.0005 0.0044 0.0013 0.0006 0.0001 0.0014 0.0001 0.0007 0.0044 0.0025 0.0002 0.002 0 0.0002 0.0019 0.0048 0.0002
Bud Site 0.0064 0.0054 0.0045 0.0021 0.0115 0.0001 0.0095 0.0117 0.0019 0.0148 0.0038 0.0003 0.009 0.0005 0.0005 0.0016 0.001 0.0003
Cell Periphery 0.0004 0.0001 0.001 0.0001 0.0002 0 0.0007 0.0001 0.0001 0.0002 0.0003 0.0001 0.0005 0 0 0.0002 0.0001 0
Cytoplasm 0.1893 0.2153 0.1606 0.1485 0.0976 0.1883 0.1622 0.1869 0.119 0.1582 0.0732 0.1498 0.1774 0.2499 0.1327 0.1959 0.1846 0.1813
Cytoplasmic Foci 0.0259 0.0122 0.005 0.0454 0.0934 0.0129 0.0261 0.0041 0.0108 0.0125 0.0339 0.0061 0.0101 0.0054 0.0052 0.0071 0.0146 0.0055
Eisosomes 0.0004 0.0002 0.003 0.0001 0.0001 0.0001 0.0007 0.0001 0.0001 0.0002 0.0002 0.0001 0.0002 0.0001 0.0001 0.0005 0.0006 0.0001
Endoplasmic Reticulum 0.0056 0.0048 0.0035 0.0027 0.006 0.0024 0.005 0.0028 0.0042 0.0023 0.0018 0.0016 0.004 0.0034 0.0025 0.0021 0.0031 0.0026
Endosome 0.0226 0.0079 0.0056 0.027 0.0863 0.0103 0.018 0.002 0.0113 0.0053 0.0366 0.0029 0.0162 0.004 0.0038 0.0065 0.0067 0.0049
Golgi 0.0088 0.0032 0.006 0.0078 0.0247 0.0062 0.0054 0.0001 0.0021 0.0032 0.0222 0.0031 0.0041 0.0001 0.0012 0.0015 0.0018 0.0006
Lipid Particles 0.0209 0.0114 0.0072 0.0075 0.0314 0.0048 0.0214 0.0004 0.0029 0.0292 0.0292 0.0038 0.005 0.0006 0.0015 0.0047 0.0102 0.0012
Mitochondria 0.0058 0.0057 0.0046 0.0357 0.0063 0.0014 0.0129 0.0009 0.0111 0.0078 0.0541 0.0016 0.0071 0.0005 0.0024 0.0025 0.0071 0.0065
None 0.369 0.4071 0.4437 0.2746 0.2069 0.3691 0.3721 0.522 0.4559 0.4834 0.4545 0.2726 0.3613 0.4478 0.4129 0.3929 0.3753 0.2602
Nuclear Periphery 0.0177 0.0176 0.0141 0.0174 0.0153 0.0118 0.0103 0.0119 0.0122 0.0071 0.0036 0.0073 0.0165 0.0139 0.0139 0.0117 0.0138 0.0109
Nucleolus 0.0042 0.0028 0.0038 0.0035 0.0025 0.0045 0.0048 0.0028 0.0031 0.0028 0.0037 0.0046 0.0041 0.002 0.0021 0.0036 0.002 0.0037
Nucleus 0.2246 0.2666 0.2838 0.2739 0.2718 0.3028 0.2633 0.2274 0.3184 0.1552 0.2311 0.4878 0.3361 0.2402 0.3983 0.3067 0.3194 0.482
Peroxisomes 0.0079 0.005 0.0011 0.0242 0.0292 0.0007 0.0107 0.0001 0.0124 0.052 0.0197 0.0117 0.0019 0.0002 0.0016 0.0039 0.0108 0.0011
Punctate Nuclear 0.0492 0.0276 0.0296 0.1184 0.0423 0.0725 0.0166 0.0208 0.0246 0.0286 0.008 0.0241 0.0244 0.0287 0.0156 0.0379 0.0169 0.0335
Vacuole 0.0118 0.0018 0.0015 0.0024 0.04 0.001 0.0067 0.0043 0.0039 0.0008 0.0037 0.0012 0.0053 0.0018 0.0013 0.0032 0.0018 0.0017
Vacuole Periphery 0.0042 0.0005 0.0004 0.0014 0.0187 0.0003 0.0018 0.0004 0.001 0.0003 0.0019 0.0002 0.0017 0.0003 0.0006 0.0007 0.0011 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 11.7028 11.9561 12.1989 8.4362 6.5028 13.3672 11.2073 10.3689 9.8489 12.6213
Translational Efficiency 0.3151 0.2808 0.2429 0.3281 0.3815 0.2923 0.3988 0.3155 0.3474 0.3413

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1887 1543 1080 1303 920 1711 176 2313 2807 3254 1256 3616

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 605.86 679.69 846.65 827.01 672.97 695.84 943.50 822.33 627.86 688.18 860.22 824.02
Standard Deviation 68.05 118.14 94.03 136.65 77.41 131.44 86.64 102.69 77.91 125.57 98.92 116.10
Intensity Change Log 2 — 0.165892 0.482781 0.448920 — 0.048213 0.487480 0.289175 — 0.105163 0.485256 0.367064

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000053 0.000180 0.000262 0.000944 0.000104 0.000799 0.000648 0.000602 0.000070 0.000505 0.000316 0.000725
Bud Neck 0.007387 0.012097 0.010780 0.015805 0.003393 0.018211 0.001746 0.020799 0.006078 0.015312 0.009514 0.019000
Bud Site 0.002526 0.005221 0.004415 0.039982 0.000906 0.007094 0.001276 0.015550 0.001995 0.006206 0.003975 0.024354
Cell Periphery 0.000176 0.000144 0.000097 0.000279 0.000165 0.000206 0.000197 0.000160 0.000172 0.000177 0.000111 0.000203
Cytoplasm 0.384082* 0.320894* 0.058057 0.229567* 0.328719* 0.277811* 0.090002 0.173395* 0.365936* 0.298240* 0.062533 0.193636*
Cytoplasmic Foci 0.071857 0.061541 0.001067 0.011670 0.113603 0.117618 0.009173 0.002040 0.085540 0.091027 0.002203 0.005510
Eisosomes 0.000071 0.000053 0.000019 0.000045 0.000086 0.000091 0.000097 0.000021 0.000076 0.000073 0.000030 0.000030
Endoplasmic Reticulum 0.000458 0.001501 0.004454 0.004460 0.001073 0.001344 0.010845 0.002723 0.000660 0.001418 0.005349 0.003349
Endosome 0.002466 0.005188 0.000594 0.013395 0.003737 0.009253 0.002597 0.001891 0.002883 0.007325 0.000874 0.006036
Golgi 0.000738 0.000778 0.000033 0.013725 0.000874 0.003150 0.000084 0.001348 0.000783 0.002025 0.000040 0.005808
Lipid Particles 0.004375 0.004611 0.000422 0.001485 0.008393 0.009267 0.003104 0.000437 0.005692 0.007059 0.000798 0.000815
Mitochondria 0.002683 0.005602 0.000891 0.005109 0.002391 0.017696 0.000458 0.004760 0.002587 0.011961 0.000830 0.004886
Mitotic Spindle 0.001804 0.002687 0.001104 0.085967 0.003624 0.011009 0.000164 0.017930 0.002400 0.007063 0.000972 0.042447
None 0.013985 0.016344 0.002979 0.008956 0.006732 0.007189 0.003085 0.006230 0.011608 0.011530 0.002994 0.007212
Nuclear Periphery 0.000843 0.001399 0.001559 0.002749 0.001181 0.001422 0.002150 0.002451 0.000953 0.001411 0.001642 0.002559
Nuclear Periphery Foci 0.000454 0.001049 0.000217 0.003905 0.000716 0.000506 0.001775 0.000530 0.000540 0.000764 0.000435 0.001747
Nucleolus 0.002043 0.004802 0.000660 0.004007 0.002537 0.005051 0.001016 0.001373 0.002205 0.004933 0.000710 0.002322
Nucleus 0.492042* 0.530586* 0.903746* 0.479238* 0.502636* 0.465693* 0.821252* 0.727427* 0.495514* 0.496464* 0.892186* 0.637994*
Peroxisomes 0.002198 0.004301 0.000093 0.001212 0.003830 0.014535 0.000375 0.000272 0.002733 0.009682 0.000133 0.000611
Vacuole 0.009058 0.019993 0.008403 0.070459 0.014791 0.029451 0.049776 0.019013 0.010937 0.024966 0.014201 0.037551
Vacuole Periphery 0.000700 0.001029 0.000149 0.007040 0.000511 0.002604 0.000181 0.001048 0.000638 0.001857 0.000153 0.003207

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -5.09 -7.71 -9.66 -7.39 -4.32 -2.17 -8.53 -4.05 0.99 3.28 -2.64 -9.77 -8.00 -0.35 -1.49
Bud Neck -2.96 -2.33 -3.57 -0.09 -0.33 -9.73 1.99 -11.34 -0.65 -12.48 -8.05 -2.80 -9.86 -1.12 -3.83
Bud Site -2.67 -1.92 -7.92 -6.63 -6.45 -6.91 -4.89 -7.95 -2.63 -7.14 -5.95 -2.38 -10.53 -6.69 -6.85
Cell Periphery 1.84 4.84 -0.85 -2.57 -5.32 -2.57 -2.54 4.05 5.32 5.31 -0.66 4.67 2.39 2.93 -2.51
Cytoplasm 7.47 50.38 22.37 13.27 -19.77 5.05 19.25 19.98 18.75 -5.79 10.73 54.29 35.03 23.75 -22.52
Cytoplasmic Foci 2.20 25.83 22.92 17.54 -8.84 -0.70 18.68 21.54 29.06 2.72 -1.80 32.62 32.15 33.61 -3.54
Eisosomes 4.05 13.74 2.34 -2.17 -14.10 -0.60 -3.22 12.62 15.38 9.76 0.62 11.54 13.28 12.75 -0.75
Endoplasmic Reticulum -9.79 -17.51 -16.17 -12.38 0.53 -1.82 -10.34 -15.40 -12.89 8.07 -8.90 -19.47 -23.96 -17.85 7.14
Endosome -3.36 5.50 -3.93 -0.55 -6.50 -6.82 1.20 5.57 11.06 5.12 -7.83 7.09 0.11 7.51 -5.64
Golgi -0.16 4.26 -3.06 -3.03 -3.64 -3.38 2.55 0.78 4.04 -2.02 -3.51 4.93 -2.49 -0.08 -4.15
Lipid Particles -0.36 9.68 7.18 5.65 -7.26 -0.70 5.63 9.79 14.96 7.74 -2.36 12.46 12.80 15.19 0.82
Mitochondria -2.41 3.62 -0.12 2.38 -4.19 -9.13 4.64 -1.34 8.53 -5.73 -8.85 4.82 -1.01 8.37 -6.08
Mitotic Spindle -0.86 0.25 -10.39 -10.23 -10.48 -3.08 2.30 -5.62 -2.27 -9.13 -3.77 1.25 -12.32 -9.63 -13.09
None -0.96 8.55 5.17 5.38 -4.48 -0.61 5.52 0.05 0.80 -8.59 0.14 9.63 5.34 5.24 -9.76
Nuclear Periphery -2.63 -6.21 -10.67 -9.26 -7.50 -1.58 -7.34 -14.63 -14.38 -5.38 -3.22 -8.04 -17.13 -16.69 -11.86
Nuclear Periphery Foci -1.96 0.54 -3.38 -2.30 -3.91 1.87 -4.47 1.62 -0.18 4.97 -1.20 -0.51 -2.58 -1.83 -2.69
Nucleolus -3.30 4.82 -0.21 2.62 -2.64 -4.46 4.42 3.09 7.66 -2.17 -5.54 6.52 1.81 6.68 -3.79
Nucleus -3.82 -50.59 -5.40 -1.80 33.07 3.02 -15.21 -21.73 -30.29 2.20 -0.12 -53.62 -24.39 -24.10 29.42
Peroxisomes -2.86 5.54 4.26 5.92 -4.36 -7.81 6.00 6.38 11.74 4.16 -8.82 8.19 7.67 13.12 -3.43
Vacuole -8.43 -2.91 -20.88 -18.14 -19.88 -7.86 -7.15 -10.36 -3.20 4.84 -12.58 -6.32 -23.23 -16.07 -14.68
Vacuole Periphery -1.19 2.64 -3.39 -2.93 -4.68 -5.73 4.60 -0.11 5.31 -2.16 -4.89 3.38 -2.51 1.91 -5.23
Standard name
Human Ortholog
Description Checkpoint protein; involved in the activation of the DNA damage and meiotic pachytene checkpoints; subunit of a clamp loader that loads Rad17p-Mec3p-Ddc1p onto DNA; homolog of human and S. pombe Rad17 protein
Localization
Cell Percentages nucleus (20%), cytoplasm (9%), mixed (56%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Rad24

Rad24


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rad24-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available