Standard name | |
---|---|
Human Ortholog | |
Description | Trans-aconitate methyltransferase; cytosolic enzyme that catalyzes the methyl esterification of 3-isopropylmalate, an intermediate of the leucine biosynthetic pathway, and trans-aconitate, which inhibits the citric acid cycle |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 3 | 2 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
Bud | 0 | 4 | 0 | 0 | 0 | 0 | 0 | 2 | 3 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 18 | 2 | 2 | 9 | 8 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 2 | 2 | 3 | 3 | 5 | 7 | 7 | 4 | 6 | 6 | 8 | 1 | 2 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 151 | 441 | 152 | 151 | 222 | 187 | 248 | 240 | 247 | 190 | 191 | 179 | 203 | 231 | 345 | 273 | 158 | 180 | 140 | 405 | 140 | 152 | 280 | 271 |
Endoplasmic Reticulum | 1 | 0 | 1 | 0 | 4 | 12 | 20 | 5 | 7 | 9 | 6 | 6 | 1 | 1 | 0 | 11 | 8 | 3 | 0 | 0 | 0 | 0 | 0 | 3 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 7 | 20 | 17 | 26 | 36 | 0 | 0 | 0 | 4 | 1 | 0 | 0 | 2 | 1 | 0 | 0 | 3 |
Nucleus | 0 | 0 | 0 | 1 | 1 | 1 | 4 | 6 | 4 | 8 | 0 | 7 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 10 | 2 | 5 | 4 | 2 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 1 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 1 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 3 | 0 | 0 | 0 | 1 |
Vac/Vac Membrane | 0 | 5 | 5 | 6 | 9 | 16 | 22 | 74 | 55 | 39 | 30 | 47 | 0 | 5 | 5 | 1 | 1 | 4 | 0 | 4 | 2 | 2 | 5 | 2 |
Unique Cell Count | 153 | 448 | 159 | 156 | 238 | 218 | 294 | 333 | 327 | 260 | 250 | 267 | 204 | 237 | 347 | 274 | 159 | 184 | 150 | 450 | 155 | 169 | 307 | 298 |
Labelled Cell Count | 153 | 453 | 160 | 161 | 240 | 222 | 303 | 346 | 344 | 274 | 262 | 290 | 205 | 244 | 352 | 290 | 169 | 188 | 150 | 450 | 155 | 169 | 307 | 298 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 38.7 | 17.9 | 29.2 | 30.3 | 26.4 | 21.6 | 17.2 | 16.1 | 13.4 | 12.6 | 12.3 | 11.5 | 29.6 | 29.4 | 31.9 | 54.5 | 74.6 | 75.7 | 26.7 | 29.2 | 25.7 |
Std Deviation (1e-4) | 13.3 | 7.0 | 11.0 | 10.4 | 7.5 | 6.7 | 5.2 | 5.4 | 4.8 | 4.5 | 4.4 | 4.9 | 11.0 | 10.0 | 10.2 | 22.2 | 29.1 | 31.9 | 11.2 | 11.0 | 11.3 |
Intensity Change (Log2) | – | – | – | 0.06 | -0.14 | -0.43 | -0.76 | -0.86 | -1.13 | -1.22 | -1.25 | -1.35 | 0.02 | 0.01 | 0.13 | 0.9 | 1.35 | 1.38 | -0.13 | 0.0 | -0.18 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.6 | -1.0 | -3.1 | -3.6 | -6.1 | -5.4 | -5.8 | -5.1 | -6.8 | 2.5 | 1.0 | 3.0 | 3.0 | 2.1 | 1.2 |
Endoplasmic Reticulum | 0 | 0 | 2.6 | 3.0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 3.2 | 3.3 | 4.2 | 4.8 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0.3 | 0.3 | 1.8 | 1.9 | 5.4 | 4.3 | 3.8 | 3.1 | 4.4 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 36.2918 | 52.1901 | 43.3439 | 39.3672 | 38.5488 | 46.2647 | 26.5502 | 37.8564 | 32.1733 | 34.8797 | 24.9303 | 35.5016 | 30.6395 | 41.6708 | 37.9495 | 33.5671 | 34.452 | 38.327 |
Actin | 0.0389 | 0.0004 | 0.002 | 0.0319 | 0.014 | 0.0034 | 0.0147 | 0.0023 | 0.0041 | 0.0063 | 0.01 | 0.0023 | 0.0127 | 0.0016 | 0.0007 | 0.0011 | 0.0027 | 0.0014 |
Bud | 0.0008 | 0.0003 | 0.0004 | 0.0009 | 0.0061 | 0.0002 | 0.0007 | 0.0008 | 0.0011 | 0.0014 | 0.0003 | 0.0001 | 0.001 | 0.0016 | 0.0003 | 0.0002 | 0.0137 | 0.0004 |
Bud Neck | 0.0003 | 0.0001 | 0.0002 | 0.0003 | 0.0005 | 0.0005 | 0.0007 | 0.0002 | 0.0003 | 0.0008 | 0.0004 | 0.0005 | 0.0005 | 0.0002 | 0.0002 | 0.0003 | 0.0003 | 0.0007 |
Bud Periphery | 0.0009 | 0 | 0.0001 | 0.0011 | 0.0148 | 0.0001 | 0.0005 | 0.0001 | 0.0009 | 0.0006 | 0.0003 | 0.0001 | 0.001 | 0.0004 | 0.0001 | 0 | 0.0041 | 0.0001 |
Bud Site | 0.0013 | 0.0002 | 0.0005 | 0.0021 | 0.0036 | 0.0001 | 0.001 | 0.0005 | 0.0022 | 0.0042 | 0.0007 | 0.0001 | 0.0008 | 0.0028 | 0.0002 | 0.0001 | 0.0005 | 0.0001 |
Cell Periphery | 0.0002 | 0 | 0.0001 | 0.0001 | 0.0013 | 0 | 0.0002 | 0.0001 | 0.0003 | 0.0003 | 0.0001 | 0 | 0.0002 | 0.0001 | 0 | 0 | 0.0002 | 0 |
Cytoplasm | 0.8227 | 0.993 | 0.9707 | 0.9022 | 0.7662 | 0.9481 | 0.8274 | 0.9852 | 0.9456 | 0.9061 | 0.9291 | 0.9575 | 0.8636 | 0.9856 | 0.9751 | 0.9682 | 0.9393 | 0.9561 |
Cytoplasmic Foci | 0.0105 | 0.0004 | 0.001 | 0.0097 | 0.0131 | 0.0053 | 0.0167 | 0.0011 | 0.005 | 0.024 | 0.0071 | 0.0052 | 0.0216 | 0.0011 | 0.0054 | 0.0111 | 0.0072 | 0.0067 |
Eisosomes | 0.0003 | 0 | 0.0001 | 0.0003 | 0.0005 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0 |
Endoplasmic Reticulum | 0.0072 | 0.0005 | 0.0021 | 0.0013 | 0.0041 | 0.0006 | 0.0159 | 0.0015 | 0.0034 | 0.0028 | 0.0066 | 0.0013 | 0.0028 | 0.0002 | 0.0003 | 0.0006 | 0.0037 | 0.001 |
Endosome | 0.0093 | 0.0001 | 0.0012 | 0.0039 | 0.0308 | 0.0005 | 0.0342 | 0.0005 | 0.0111 | 0.0206 | 0.017 | 0.0042 | 0.0072 | 0.0001 | 0.0009 | 0.0011 | 0.004 | 0.0063 |
Golgi | 0.0036 | 0 | 0.0004 | 0.0047 | 0.0254 | 0.0002 | 0.0056 | 0.0001 | 0.0006 | 0.0019 | 0.0026 | 0.0055 | 0.002 | 0 | 0.0002 | 0.0004 | 0.0008 | 0.0008 |
Lipid Particles | 0.0078 | 0 | 0.0004 | 0.0094 | 0.0044 | 0.0034 | 0.0135 | 0.0001 | 0.0005 | 0.0027 | 0.0042 | 0.0017 | 0.0111 | 0 | 0.0041 | 0.0043 | 0.0041 | 0.0005 |
Mitochondria | 0.0134 | 0.0001 | 0.0012 | 0.0028 | 0.0717 | 0.0003 | 0.0045 | 0.0004 | 0.0004 | 0.0012 | 0.001 | 0.0012 | 0.0033 | 0.0001 | 0.0005 | 0.0004 | 0.0004 | 0.0019 |
None | 0.0209 | 0.0003 | 0.0088 | 0.0027 | 0.0214 | 0.0017 | 0.0056 | 0.0008 | 0.0083 | 0.0052 | 0.0033 | 0.0048 | 0.0121 | 0.0009 | 0.0009 | 0.0022 | 0.0074 | 0.0029 |
Nuclear Periphery | 0.0212 | 0.0009 | 0.0039 | 0.0032 | 0.0029 | 0.0039 | 0.0158 | 0.0013 | 0.0024 | 0.0026 | 0.0054 | 0.0029 | 0.0096 | 0.0007 | 0.0013 | 0.0011 | 0.0018 | 0.0021 |
Nucleolus | 0.0008 | 0 | 0 | 0.0001 | 0.0008 | 0.0045 | 0.0012 | 0 | 0.0002 | 0.0005 | 0.0001 | 0 | 0.0017 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0001 |
Nucleus | 0.0246 | 0.0033 | 0.0056 | 0.015 | 0.0083 | 0.0145 | 0.0223 | 0.0043 | 0.008 | 0.0067 | 0.0073 | 0.01 | 0.0262 | 0.0039 | 0.0071 | 0.006 | 0.0062 | 0.0113 |
Peroxisomes | 0.0047 | 0 | 0.0001 | 0.0047 | 0.0011 | 0.0002 | 0.0045 | 0.0001 | 0.0002 | 0.0005 | 0.0008 | 0.0011 | 0.0079 | 0 | 0.0003 | 0.0007 | 0.0005 | 0.0002 |
Punctate Nuclear | 0.0049 | 0.0001 | 0.0004 | 0.0011 | 0.0008 | 0.0116 | 0.0076 | 0.0003 | 0.001 | 0.0018 | 0.0019 | 0.001 | 0.009 | 0.0003 | 0.0005 | 0.0018 | 0.0009 | 0.0056 |
Vacuole | 0.0035 | 0.0003 | 0.0004 | 0.0018 | 0.005 | 0.0007 | 0.005 | 0.0003 | 0.0036 | 0.0074 | 0.0011 | 0.0004 | 0.0041 | 0.0003 | 0.001 | 0.0002 | 0.0015 | 0.0013 |
Vacuole Periphery | 0.0022 | 0.0001 | 0.0003 | 0.0006 | 0.0031 | 0.0003 | 0.0022 | 0.0001 | 0.0006 | 0.0027 | 0.0005 | 0.0001 | 0.0017 | 0.0001 | 0.0006 | 0.0001 | 0.0003 | 0.0005 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 14.0991 | 23.0154 | 8.9459 | 17.2378 | 8.7426 | 41.6611 | 49.0351 | 57.9637 | 50.1673 | 38.1306 |
Translational Efficiency | 0.8792 | 0.7055 | 2.0882 | 1.2015 | 1.4813 | 1.1599 | 1.2717 | 1.0385 | 1.1246 | 0.9885 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1480 | 502 | 309 | 1556 | 1549 | 1934 | 2294 | 1783 | 3029 | 2436 | 2603 | 3339 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 2641.27 | 3673.27 | 1518.38 | 802.65 | 3177.76 | 3138.64 | 880.50 | 822.93 | 2915.63 | 3248.81 | 956.22 | 813.48 |
Standard Deviation | 899.79 | 1018.55 | 515.06 | 195.93 | 916.52 | 922.21 | 159.54 | 115.39 | 947.15 | 967.35 | 310.63 | 158.43 |
Intensity Change Log 2 | — | 0.475833 | -0.798699 | -1.718389 | — | -0.017871 | -1.851615 | -1.949169 | — | 0.227281 | -1.278418 | -1.839824 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000111 | 0.000141 | 0.000422 | 0.000599 | 0.000051 | 0.000100 | 0.000190 | 0.000243 | 0.000081 | 0.000109 | 0.000218 | 0.000409 |
Bud Neck | 0.000331 | 0.001087 | 0.004149 | 0.010692 | 0.000291 | 0.000565 | 0.005469 | 0.008044 | 0.000310 | 0.000673 | 0.005313 | 0.009278 |
Bud Site | 0.000929 | 0.002085 | 0.004595 | 0.039406 | 0.001101 | 0.002535 | 0.011260 | 0.035702 | 0.001017 | 0.002442 | 0.010469 | 0.037428 |
Cell Periphery | 0.000340 | 0.000490 | 0.000274 | 0.000192 | 0.000193 | 0.000169 | 0.000118 | 0.000172 | 0.000265 | 0.000235 | 0.000137 | 0.000181 |
Cytoplasm | 0.777779* | 0.690292* | 0.509738* | 0.506840* | 0.738400* | 0.777890* | 0.471092* | 0.526052* | 0.757641* | 0.759838* | 0.475680* | 0.517099* |
Cytoplasmic Foci | 0.004466 | 0.012324 | 0.012381 | 0.021033 | 0.002911 | 0.006323 | 0.006657 | 0.017735 | 0.003671 | 0.007560 | 0.007337 | 0.019272 |
Eisosomes | 0.000001 | 0.000001 | 0.000027 | 0.000041 | 0.000000 | 0.000001 | 0.000022 | 0.000034 | 0.000001 | 0.000001 | 0.000022 | 0.000037 |
Endoplasmic Reticulum | 0.000086 | 0.000177 | 0.001660 | 0.001568 | 0.000026 | 0.000085 | 0.001300 | 0.001307 | 0.000055 | 0.000104 | 0.001343 | 0.001428 |
Endosome | 0.000076 | 0.000169 | 0.001923 | 0.006121 | 0.000025 | 0.000230 | 0.001221 | 0.003801 | 0.000050 | 0.000218 | 0.001304 | 0.004882 |
Golgi | 0.000582 | 0.000854 | 0.000437 | 0.003668 | 0.000462 | 0.000643 | 0.000076 | 0.001740 | 0.000521 | 0.000686 | 0.000119 | 0.002639 |
Lipid Particles | 0.000335 | 0.000561 | 0.001376 | 0.001577 | 0.000104 | 0.000191 | 0.000668 | 0.000813 | 0.000217 | 0.000267 | 0.000752 | 0.001169 |
Mitochondria | 0.000103 | 0.000147 | 0.000558 | 0.004023 | 0.000135 | 0.000262 | 0.000783 | 0.002273 | 0.000119 | 0.000238 | 0.000756 | 0.003089 |
Mitotic Spindle | 0.000567 | 0.003858 | 0.011571 | 0.035733 | 0.000302 | 0.002431 | 0.008402 | 0.017973 | 0.000431 | 0.002725 | 0.008778 | 0.026249 |
None | 0.024071 | 0.015849 | 0.010563 | 0.007426 | 0.018818 | 0.019403 | 0.007266 | 0.006411 | 0.021385 | 0.018670 | 0.007658 | 0.006884 |
Nuclear Periphery | 0.000059 | 0.000135 | 0.001063 | 0.002092 | 0.000060 | 0.000079 | 0.001497 | 0.001216 | 0.000060 | 0.000090 | 0.001446 | 0.001624 |
Nuclear Periphery Foci | 0.001340 | 0.001736 | 0.002105 | 0.000875 | 0.000681 | 0.001071 | 0.000509 | 0.000533 | 0.001003 | 0.001208 | 0.000699 | 0.000692 |
Nucleolus | 0.000070 | 0.000103 | 0.000520 | 0.000867 | 0.000056 | 0.000139 | 0.000407 | 0.000478 | 0.000063 | 0.000131 | 0.000420 | 0.000659 |
Nucleus | 0.185117* | 0.264908* | 0.378037* | 0.257131* | 0.234181* | 0.183558* | 0.447643* | 0.289573* | 0.210208* | 0.200322* | 0.439380* | 0.274455* |
Peroxisomes | 0.000006 | 0.000017 | 0.000139 | 0.000871 | 0.000004 | 0.000031 | 0.000244 | 0.000521 | 0.000005 | 0.000028 | 0.000232 | 0.000684 |
Vacuole | 0.003447 | 0.004669 | 0.058230 | 0.095193 | 0.001639 | 0.003459 | 0.034959 | 0.082782 | 0.002522 | 0.003709 | 0.037721 | 0.088566 |
Vacuole Periphery | 0.000185 | 0.000398 | 0.000232 | 0.004053 | 0.000560 | 0.000836 | 0.000216 | 0.002598 | 0.000377 | 0.000746 | 0.000218 | 0.003276 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -2.29 | -8.00 | -2.69 | -1.49 | 2.67 | -3.64 | -6.63 | -8.79 | -4.15 | -0.97 | -2.89 | -7.32 | -5.03 | -3.47 | -0.74 |
Bud Neck | -6.32 | -10.26 | -10.07 | -8.00 | -2.17 | -4.09 | -8.76 | -12.52 | -11.61 | -2.27 | -6.37 | -9.93 | -15.74 | -14.23 | -3.10 |
Bud Site | -2.97 | -9.58 | -8.24 | -7.35 | -5.16 | -2.41 | -6.05 | -8.84 | -8.19 | -5.23 | -3.49 | -7.11 | -12.09 | -10.96 | -6.59 |
Cell Periphery | -3.66 | -0.59 | 6.61 | 8.96 | 7.14 | 0.66 | 5.91 | 4.25 | 4.34 | -1.57 | 0.24 | 6.97 | 7.81 | 9.48 | 2.03 |
Cytoplasm | 11.20 | 24.40 | 44.05 | 23.04 | 1.64 | -5.43 | 46.34 | 38.73 | 44.24 | -2.58 | 1.67 | 57.24 | 58.40 | 53.97 | -1.16 |
Cytoplasmic Foci | -6.81 | -5.39 | -7.71 | 2.10 | 1.90 | -8.23 | -6.50 | -13.68 | -7.92 | -9.44 | -9.02 | -6.31 | -14.93 | -5.97 | -8.67 |
Eisosomes | -3.08 | -7.75 | -31.42* | -29.51* | -4.94 | -4.84 | -27.76 | -34.37* | -33.78* | -9.93 | -4.14 | -28.04 | -46.37* | -45.48* | -13.21 |
Endoplasmic Reticulum | -0.82 | -6.68 | -13.52 | -11.53 | -0.50 | -2.09 | -23.79 | -21.98 | -20.55 | -0.23 | -1.40 | -23.87 | -23.29 | -21.94 | -1.03 |
Endosome | -1.71 | -7.77 | -8.93 | -8.46 | -2.33 | -2.73 | -14.26 | -7.60 | -6.73 | -3.74 | -2.73 | -15.62 | -11.64 | -10.50 | -5.84 |
Golgi | -0.74 | 0.58 | -1.21 | -0.67 | -1.39 | -0.75 | 2.74 | -0.96 | -0.50 | -2.56 | -0.85 | 4.15 | -1.53 | -1.06 | -3.03 |
Lipid Particles | -1.85 | -6.88 | -5.12 | -0.59 | 3.12 | -3.53 | -14.70 | -20.30 | -15.29 | -4.36 | -1.62 | -8.43 | -10.03 | -7.41 | -3.20 |
Mitochondria | -3.72 | -11.23 | -4.39 | -4.26 | -3.16 | -1.03 | -10.83 | -3.39 | -3.05 | -1.68 | -1.30 | -12.15 | -5.48 | -4.96 | -3.14 |
Mitotic Spindle | -1.81 | -4.28 | -8.69 | -5.47 | -0.68 | -3.91 | -7.87 | -7.44 | -5.97 | -3.26 | -3.81 | -8.61 | -11.39 | -9.05 | -4.85 |
None | 4.32 | 7.84 | 9.73 | 5.99 | 2.84 | 0.40 | 12.35 | 12.94 | 13.13 | 3.12 | 2.95 | 13.92 | 15.15 | 14.69 | 3.56 |
Nuclear Periphery | -8.43 | -16.91 | -17.29 | -16.47 | -6.68 | -3.47 | -36.25* | -26.77 | -26.10 | -0.83 | -6.15 | -39.06 | -27.85 | -27.11 | -4.86 |
Nuclear Periphery Foci | -1.21 | -4.42 | 3.95 | 1.97 | 6.81 | -1.84 | 0.71 | 0.97 | 2.82 | 0.55 | -1.36 | 1.48 | 3.77 | 3.11 | 3.22 |
Nucleolus | -3.15 | -6.66 | -6.67 | -6.04 | -1.80 | -4.44 | -10.98 | -8.27 | -6.02 | -0.53 | -4.76 | -12.14 | -9.86 | -8.07 | -2.41 |
Nucleus | -9.60 | -13.50 | -17.59 | -4.82 | 3.10 | 8.00 | -33.00 | -12.56 | -19.59 | 17.27 | 0.88 | -40.58 | -21.30 | -21.17 | 19.27 |
Peroxisomes | -2.92 | -9.54 | -4.58 | -4.33 | -2.66 | -3.38 | -4.11 | -3.88 | -3.51 | -1.18 | -3.78 | -4.52 | -5.96 | -5.49 | -2.14 |
Vacuole | -3.49 | -11.09 | -30.33 | -27.27 | -4.76 | -5.65 | -24.09 | -31.67 | -30.85 | -16.00 | -5.38 | -25.96 | -43.76 | -42.37 | -20.13 |
Vacuole Periphery | -0.70 | -0.93 | -2.88 | -2.31 | -2.75 | -0.36 | 0.85 | -1.05 | -0.84 | -2.29 | -0.86 | 0.80 | -2.50 | -1.67 | -3.62 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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