Standard name
Human Ortholog
Description DNA dependent ATPase/DNA helicase; helicase belonging to the Dna2p- and Nam7p-like family of helicases that is involved in modulating translation termination; interacts with the translation termination factors, localized to polysomes
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 – 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0.06 0.05 0 0 0 0 0 0 0
Cytoplasm 0.99 0.98 0.96 0.99 0.94 0.9 0.85 0.75 0.68 – 0.54 0.53 0.98 0.99 0.99 0.86 0.93 0.93 0.89 0.86 0.72 0.83 0.73 0.69
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0.06 0 0 0 0 0 0 0.06 0.07
Endosome 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0.12 0.12 0.27 0.35 0.47 0.58 – 0.7 0.74 0 0 0 0.1 0 0 0.06 0 0.14 0.05 0.1 0.1
Nucleus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0.08 0.07 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 0 0 0 0 0 0 0 – 0 0 1 0 0 0 0 0 0 2 2 2 1 4
Bud 0 1 1 0 2 4 6 11 9 – 12 2 1 0 4 0 0 0 0 5 1 1 4 6
Bud Neck 0 1 1 0 2 0 1 0 1 – 0 0 0 0 0 0 0 0 0 1 2 1 2 2
Bud Site 0 0 0 0 0 0 1 0 0 – 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 4 4 3 4 4 3 5 3 6 – 4 4 3 2 9 6 5 4 0 0 0 0 0 3
Cytoplasm 93 332 256 214 277 279 356 380 196 – 130 147 261 285 304 89 87 85 99 311 198 181 167 213
Endoplasmic Reticulum 0 3 5 0 1 0 0 1 2 – 5 1 1 3 0 6 3 3 0 3 8 7 12 23
Endosome 0 0 0 0 3 1 0 0 1 – 0 0 0 0 0 4 1 0 1 4 3 0 2 2
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 1 0 0 2 0 0 0 1 1 0 1 2
Mitochondria 0 6 11 27 35 84 148 236 167 – 169 206 1 1 10 10 2 2 6 14 39 11 21 32
Nucleus 0 0 0 0 0 1 2 0 1 – 0 1 0 0 0 1 0 0 0 0 0 1 0 0
Nuclear Periphery 0 1 0 0 0 0 1 0 0 – 0 1 0 0 0 0 1 1 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 1 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 1 0
SpindlePole 0 0 0 0 1 1 2 3 0 – 0 1 0 0 0 0 0 0 0 2 2 0 2 1
Vac/Vac Membrane 1 0 0 0 2 0 0 6 1 – 2 1 2 0 0 8 7 4 1 10 9 5 7 11
Unique Cell Count 94 339 266 217 294 311 420 504 290 240 279 266 288 306 104 94 91 112 360 274 218 229 310
Labelled Cell Count 98 349 277 245 327 373 522 640 384 322 364 271 291 328 126 106 99 112 360 274 218 229 310


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.1 4.5 3.9 4.1 4.2 3.8 3.6 3.6 3.2 – 3.2 3.1 5.1 4.9 4.6 6.6 7.5 6.8 5.4 5.6 6.0
Std Deviation (1e-4) 0.8 0.8 0.8 1.3 1.3 1.5 1.1 1.0 0.9 – 0.6 0.8 1.0 1.0 1.0 2.2 3.5 2.7 1.6 1.3 1.6
Intensity Change (Log2) – – – 0.08 0.11 -0.02 -0.11 -0.13 -0.27 – -0.28 -0.35 0.4 0.34 0.24 0.76 0.95 0.8 0.48 0.53 0.63


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.9049 2.3547 2.672 2.3992 2.1081 2.4972 0.9472 0.2076 0.9181 0.5582 0.6121 0.1795 4.7988 4.7139 5.0887 3.8891 5.6751 4.6481
Actin 0.0825 0.0073 0.0303 0.0363 0.0142 0.0077 0.0148 0.0036 0.0329 0.0185 0.0037 0.0096 0.0897 0.0047 0.0251 0.0003 0.0035 0.0143
Bud 0.0026 0.0056 0.002 0.0285 0.0021 0.0069 0.0028 0.0084 0.0085 0.0144 0.0091 0.0025 0.0008 0.0004 0.0004 0.0001 0.0006 0.004
Bud Neck 0.0072 0.0004 0.0142 0.0054 0.0019 0.0027 0.0011 0.0005 0.0027 0.0012 0.0015 0.001 0.0006 0.0004 0.0003 0.0001 0.0006 0.0027
Bud Periphery 0.004 0.0061 0.0051 0.0234 0.0039 0.003 0.0049 0.0052 0.0051 0.0132 0.0227 0.0062 0.0019 0.0005 0.0005 0.0001 0.0008 0.0066
Bud Site 0.0227 0.0183 0.0271 0.0396 0.0193 0.0011 0.0044 0.0287 0.0117 0.0043 0.0023 0.0005 0.0019 0.0101 0.0011 0.0002 0.0012 0.0037
Cell Periphery 0.0019 0.0016 0.0024 0.0014 0.001 0.0003 0.0005 0.0007 0.0003 0.0008 0.0006 0.0002 0.0006 0.0007 0.0004 0.0002 0.0003 0.0004
Cytoplasm 0.3269 0.6125 0.6336 0.5153 0.5831 0.6934 0.5394 0.6324 0.599 0.5886 0.6369 0.6737 0.4634 0.496 0.5823 0.6492 0.5709 0.6235
Cytoplasmic Foci 0.0532 0.0137 0.0156 0.0306 0.0578 0.0298 0.0442 0.0292 0.0396 0.0557 0.0233 0.0446 0.0302 0.0098 0.0193 0.0071 0.0145 0.0215
Eisosomes 0.0007 0.0002 0.0003 0.0012 0.0004 0.0001 0.0002 0.0001 0.0001 0.0005 0.0001 0.0001 0.0007 0.0002 0.0003 0 0.0001 0.0001
Endoplasmic Reticulum 0.037 0.004 0.0081 0.0026 0.0031 0.0052 0.0166 0.0082 0.0095 0.0094 0.0039 0.0142 0.0243 0.0057 0.0147 0.0046 0.0119 0.0047
Endosome 0.0658 0.0084 0.0178 0.0221 0.0411 0.0126 0.0785 0.0366 0.04 0.0688 0.06 0.0479 0.0193 0.0086 0.0236 0.0126 0.0058 0.0142
Golgi 0.0226 0.0012 0.0055 0.0171 0.0022 0.0057 0.0103 0.0018 0.005 0.0053 0.0082 0.0022 0.0096 0.0018 0.005 0.0005 0.0023 0.0017
Lipid Particles 0.0307 0.0005 0.0029 0.0157 0.001 0.0082 0.0118 0.0006 0.0024 0.0019 0.0028 0.0021 0.0125 0.0007 0.0018 0.0002 0.0006 0.0005
Mitochondria 0.0155 0.001 0.0048 0.019 0.0126 0.0107 0.0217 0.0021 0.0038 0.0106 0.0478 0.0027 0.009 0.0003 0.001 0.0005 0.0006 0.001
None 0.2043 0.3096 0.2034 0.213 0.1821 0.1906 0.1533 0.2219 0.205 0.176 0.1434 0.1789 0.3047 0.4569 0.3142 0.3173 0.3806 0.2913
Nuclear Periphery 0.0357 0.0007 0.0017 0.0008 0.0059 0.0061 0.0343 0.0017 0.0085 0.0049 0.0025 0.002 0.0103 0.0006 0.001 0.0009 0.001 0.0004
Nucleolus 0.0024 0.0004 0.0007 0.0008 0.0023 0.0005 0.0035 0.0007 0.0007 0.0008 0.0006 0.0002 0.0003 0.0001 0.0001 0 0.0002 0.0001
Nucleus 0.0353 0.0015 0.0019 0.0014 0.0125 0.0031 0.0157 0.0036 0.006 0.0046 0.0032 0.0018 0.0024 0.0008 0.0006 0.0008 0.0009 0.0005
Peroxisomes 0.018 0.0004 0.0086 0.019 0.0381 0.005 0.0131 0.0004 0.0041 0.0016 0.0013 0.0007 0.01 0.0002 0.0033 0.0001 0.0005 0.005
Punctate Nuclear 0.0129 0.0008 0.0012 0.0017 0.0011 0.0032 0.0057 0.0024 0.0061 0.0048 0.0006 0.002 0.0024 0.0003 0.0013 0.0002 0.0015 0.0013
Vacuole 0.0134 0.0054 0.0116 0.0042 0.0118 0.0026 0.0171 0.0094 0.0073 0.0113 0.0191 0.0055 0.0036 0.0011 0.0032 0.0042 0.0012 0.0018
Vacuole Periphery 0.0046 0.0004 0.0014 0.0008 0.0024 0.0013 0.0059 0.0019 0.0018 0.0028 0.0064 0.0015 0.0019 0.0002 0.0007 0.0009 0.0002 0.0006

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 17.8656 15.2381 14.7865 21.0425 22.3135 13.7603 20.3702 23.8974 23.1217 21.4712
Translational Efficiency 0.8727 1.0811 1.0543 0.7386 0.7017 0.9917 0.6701 0.5719 0.5475 0.7453

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1678 1205 392 775 1245 1428 229 445 2923 2633 621 1220

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 630.29 720.88 1002.84 1264.73 602.53 727.85 1212.11 1064.96 618.47 724.66 1080.01 1191.86
Standard Deviation 69.01 98.34 148.38 165.24 75.63 97.61 143.99 210.65 73.20 98.01 178.15 206.83
Intensity Change Log 2 — 0.193743 0.670004 1.004742 — 0.272608 1.008416 0.821694 — 0.232827 0.845312 0.918176

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000101 0.000442 0.000392 0.001215 0.000127 0.000690 0.000907 0.000303 0.000112 0.000577 0.000582 0.000882
Bud Neck 0.020241 0.031096 0.002718 0.002686 0.018988 0.038211 0.002573 0.005346 0.019708 0.034955 0.002665 0.003656
Bud Site 0.007813 0.025676 0.009130 0.043988 0.005214 0.026213 0.027647 0.021198 0.006706 0.025967 0.015958 0.035675
Cell Periphery 0.000160 0.000165 0.000171 0.000366 0.000108 0.000230 0.000206 0.000225 0.000138 0.000200 0.000184 0.000315
Cytoplasm 0.496599* 0.392141* 0.519834* 0.656368* 0.483748* 0.398812* 0.561542* 0.391459* 0.491126* 0.395759* 0.535214* 0.559741*
Cytoplasmic Foci 0.255699* 0.291621* 0.063593 0.105493 0.239333* 0.246775* 0.171587 0.082391 0.248728* 0.267299* 0.103417 0.097067
Eisosomes 0.000216 0.000140 0.000053 0.000010 0.000141 0.000184 0.000031 0.000046 0.000184 0.000164 0.000045 0.000023
Endoplasmic Reticulum 0.001488 0.000941 0.003807 0.000706 0.001443 0.001184 0.001940 0.005020 0.001469 0.001073 0.003119 0.002280
Endosome 0.022976 0.047022 0.011963 0.017686 0.021696 0.046287 0.018679 0.032830 0.022431 0.046623 0.014439 0.023210
Golgi 0.004217 0.011135 0.000357 0.014296 0.003594 0.012838 0.001465 0.002649 0.003952 0.012058 0.000766 0.010048
Lipid Particles 0.010870 0.007965 0.003711 0.004475 0.009878 0.007149 0.004696 0.005753 0.010448 0.007522 0.004075 0.004941
Mitochondria 0.008147 0.020281 0.000174 0.001164 0.004306 0.015703 0.000024 0.005905 0.006511 0.017798 0.000119 0.002893
Mitotic Spindle 0.001534 0.004109 0.001416 0.010020 0.001277 0.007371 0.003683 0.000971 0.001424 0.005878 0.002252 0.006720
None 0.003373 0.001230 0.002137 0.000073 0.005862 0.003299 0.000189 0.005733 0.004433 0.002352 0.001419 0.002137
Nuclear Periphery 0.000426 0.000350 0.001349 0.000076 0.000796 0.000394 0.000202 0.000597 0.000584 0.000374 0.000926 0.000266
Nuclear Periphery Foci 0.000228 0.000418 0.008241 0.001344 0.000487 0.000293 0.011105 0.002476 0.000338 0.000350 0.009297 0.001757
Nucleolus 0.000897 0.001558 0.000343 0.000128 0.001924 0.001528 0.000093 0.001778 0.001334 0.001542 0.000251 0.000730
Nucleus 0.085493 0.049642 0.171644* 0.002666 0.104025 0.055103 0.009288 0.031025 0.093386 0.052603 0.111774 0.013010
Peroxisomes 0.004342 0.017282 0.000481 0.002218 0.004361 0.010303 0.001017 0.004855 0.004350 0.013497 0.000678 0.003180
Vacuole 0.072740 0.091566 0.198042 0.127802 0.090754 0.121077 0.182341 0.394486* 0.080413 0.107571 0.192252 0.225076*
Vacuole Periphery 0.002439 0.005221 0.000445 0.007219 0.001938 0.006357 0.000785 0.004953 0.002225 0.005837 0.000570 0.006392

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -7.94 -8.78 -4.16 -2.85 -2.81 -2.19 -6.20 -2.77 1.41 4.13 -3.31 -9.54 -4.50 -1.40 -1.48
Bud Neck -4.40 14.25 14.88 13.17 1.07 -7.22 9.93 7.49 14.32 -2.69 -8.49 17.01 15.79 20.09 -1.33
Bud Site -8.63 -0.81 -10.95 -4.84 -10.38 -10.79 -8.52 -5.67 1.44 1.77 -13.38 -6.77 -12.36 -3.48 -7.38
Cell Periphery -0.06 -1.35 -4.30 -3.09 -3.78 -5.04 -5.13 -3.91 0.44 -0.42 -2.70 -4.55 -5.52 -2.63 -3.62
Cytoplasm 10.18 1.87 -13.31 -20.22 -10.65 7.89 -3.06 4.72 -0.18 6.01 12.99 -0.29 -6.88 -15.58 -4.71
Cytoplasmic Foci -4.38 32.28 23.31 24.41 -7.94 -0.86 8.08 20.46 21.36 7.48 -3.18 27.08 30.62 31.57 -0.41
Eisosomes 5.19 11.21 15.59 19.74 12.49 -2.75 10.45 9.02 11.13 -2.00 1.85 14.54 18.26 19.37 8.91
Endoplasmic Reticulum 1.40 -5.61 2.14 2.00 9.81 0.77 -0.76 -2.46 -2.65 -2.21 1.44 -5.57 -1.25 -2.12 2.40
Endosome -8.64 7.76 3.29 10.63 -3.45 -9.35 2.50 -2.63 3.73 -4.00 -12.68 7.56 0.24 10.87 -5.22
Golgi -7.10 10.46 -3.58 -0.72 -5.30 -8.42 5.51 1.64 9.07 -2.55 -10.90 11.75 -3.30 1.78 -5.56
Lipid Particles 2.68 8.73 6.68 3.36 -1.98 3.24 6.10 3.00 1.15 -0.90 4.24 10.82 7.04 3.40 -1.93
Mitochondria -4.37 6.67 5.35 7.64 -1.43 -7.10 9.95 -0.47 3.55 -2.24 -7.13 8.98 3.25 8.89 -2.62
Mitotic Spindle -2.39 -0.23 -3.55 -2.37 -3.57 -4.00 -2.48 0.38 4.34 2.80 -4.60 -2.03 -3.44 -0.28 -2.42
None 2.99 2.24 4.76 7.50 6.59 2.21 5.95 0.25 -0.66 -1.77 3.02 5.58 2.12 0.37 -0.76
Nuclear Periphery 0.63 -5.31 3.42 4.74 7.79 1.64 2.40 0.68 -0.63 -1.22 1.65 -3.04 2.28 1.11 5.39
Nuclear Periphery Foci -3.35 -10.84 -8.10 -6.58 9.18 2.39 -6.38 -3.81 -4.19 4.92 -0.21 -11.68 -6.79 -6.69 9.47
Nucleolus -2.14 7.22 11.28 4.45 5.06 0.75 6.41 0.31 -0.13 -2.36 -1.02 8.29 2.21 2.61 -1.84
Nucleus 8.88 -8.21 31.46 16.62 15.70 10.66 24.05 15.32 6.20 -5.68 13.67 -3.04 32.29 17.62 13.25
Peroxisomes -8.28 9.04 4.77 10.03 -9.60 -7.68 8.51 -0.21 4.10 -3.01 -10.97 12.38 2.39 11.57 -5.24
Vacuole -4.89 -14.04 -10.23 -6.50 7.88 -5.31 -9.14 -18.73 -16.66 -8.29 -7.92 -16.61 -18.93 -14.92 0.58
Vacuole Periphery -5.30 11.74 -2.97 -0.95 -4.50 -7.55 8.73 -2.46 1.35 -3.62 -9.01 14.83 -3.76 -0.04 -5.61
Standard name
Human Ortholog
Description DNA dependent ATPase/DNA helicase; helicase belonging to the Dna2p- and Nam7p-like family of helicases that is involved in modulating translation termination; interacts with the translation termination factors, localized to polysomes
Localization
Cell Percentages cytoplasm (59%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Ecm32

Ecm32


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ecm32-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available