Standard name
Human Ortholog
Description Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0 0 0 0 0 0 0 0 0 0
Bud 0.05 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.22 0.11 0.13 0 0 0 0 0 0
Cytoplasm 0 0.08 0.1 0.12 0.14 0.06 0.05 0.05 0 0 0.05 0 0.14 0.17 0.21 0.16 0.08 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0.13 0 0 0.08 0 0 0 0 0 0 0
Endosome 0 0 0.12 0.06 0.21 0.09 0.1 0.1 0 0 0 0 0.11 0.1 0.16 0.09 0.12 0.11 0.14 0.12 0.09 0.05 0.07 0.06
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.06 0 0.09 0.26 0.26 0 0 0 0 0 0.05
Mitochondria 0.93 0.85 0.46 0.69 0.53 0.74 0.7 0.65 0.9 0.88 0.92 0.91 0.6 0.5 0.38 0.06 0.05 0.07 0.7 0.57 0.76 0.73 0.68 0.66
Nucleus 0 0 0 0 0 0.06 0.08 0.08 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0.07 0 0 0 0 0 0 0 0.09 0 0.05 0.23 0.12 0 0.05 0 0.06 0 0 0.05
SpindlePole 0 0 0.18 0.16 0.11 0.07 0.09 0.13 0.07 0.06 0.05 0 0.06 0 0 0.06 0.05 0.07 0 0 0 0 0 0
Vac/Vac Membrane 0.07 0.11 0.12 0 0.18 0.14 0.1 0.13 0.05 0.06 0 0.07 0.08 0.08 0.16 0.14 0.23 0.34 0 0.11 0 0 0.07 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 2 0 3 6 1 1 1 1 0 0 0 0 5 16 0 1 1 4 1 2 2 4 10 10
Bud 5 0 6 11 0 7 18 10 22 17 22 21 5 4 1 2 3 3 0 0 0 1 3 5
Bud Neck 0 0 2 2 0 0 0 0 0 2 2 0 0 1 0 0 0 0 0 0 0 2 4 3
Bud Site 0 2 0 0 2 0 8 5 7 7 2 3 0 0 0 1 0 1 – – – – – –
Cell Periphery 1 2 3 4 4 3 3 2 1 5 4 1 7 6 8 15 7 13 0 0 1 0 1 2
Cytoplasm 2 7 14 33 38 27 28 22 22 15 20 11 26 36 44 11 5 3 0 1 0 0 6 7
Endoplasmic Reticulum 2 5 2 2 0 1 3 1 1 5 0 2 4 28 9 2 5 3 0 0 0 2 3 5
Endosome 1 0 17 18 59 42 58 50 5 6 3 14 20 20 34 6 8 11 17 13 20 9 27 22
Golgi 1 0 3 5 1 0 1 3 0 0 1 0 11 12 7 6 17 27 2 3 2 6 12 16
Mitochondria 100 77 65 194 148 361 412 318 465 431 406 437 108 104 79 4 3 7 87 64 177 138 265 246
Nucleus 2 0 3 6 6 30 46 39 17 17 11 7 1 3 1 1 2 3 0 2 0 1 2 2
Nuclear Periphery 0 0 0 1 1 2 11 10 11 18 17 8 0 0 1 0 3 2 0 4 2 0 3 2
Nucleolus 1 0 1 0 1 9 20 16 15 7 10 11 0 0 0 0 1 0 0 2 0 1 1 2
Peroxisomes 1 0 3 12 19 10 8 9 5 5 1 7 17 2 10 16 8 4 6 1 13 5 7 19
SpindlePole 4 0 26 44 31 32 56 62 35 29 21 21 11 8 9 4 3 7 1 0 2 4 8 6
Vac/Vac Membrane 8 10 17 11 51 70 61 63 27 28 18 35 15 16 34 10 15 35 4 12 5 6 27 11
Unique Cell Count 108 91 141 281 278 488 590 487 515 488 442 480 180 208 209 69 66 102 125 114 234 190 392 371
Labelled Cell Count 130 103 165 349 362 595 734 611 633 592 538 578 230 256 237 79 81 123 125 114 234 190 392 371


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.5 4.4 5.5 5.3 6.3 5.2 5.2 5.5 4.5 4.7 4.8 4.8 5.6 5.6 5.6 5.5 6.6 7.0 6.2 6.3 6.6
Std Deviation (1e-4) 0.5 0.8 1.1 1.1 1.7 1.4 1.2 1.2 1.2 1.1 1.5 1.1 1.0 0.8 1.2 1.4 1.5 1.3 1.6 1.6 2.1
Intensity Change (Log2) – – – -0.06 0.18 -0.09 -0.09 -0.02 -0.3 -0.23 -0.21 -0.19 0.03 0.01 0.01 -0.02 0.26 0.34 0.16 0.18 0.26


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 0.5 2.0 4.2

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.6142 0.838 0.6803 0.3742 -0.0463 0.6584 3.378 3.6395 3.7691 2.9717 3.6384 3.6895 1.274 1.8298 1.1805 1.2425 0.8378 1.2608
Actin 0.0249 0.0082 0.0173 0.0025 0.0159 0.0088 0.0206 0.0338 0.0066 0.0101 0.0451 0.0111 0.0314 0.008 0.0138 0.0298 0.0041 0.0045
Bud 0.0006 0.002 0.0007 0.0005 0.0014 0.0003 0.001 0.0075 0.0023 0.0023 0.0009 0.0006 0.0014 0.005 0.0014 0.001 0.0005 0.0004
Bud Neck 0.0026 0.0018 0.0004 0.0002 0.001 0.0051 0.003 0.0017 0.0022 0.0017 0.0029 0.0041 0.0019 0.0011 0.0062 0.0032 0.0004 0.0016
Bud Periphery 0.001 0.0039 0.0012 0.0011 0.0029 0.0009 0.0024 0.014 0.0063 0.0105 0.0016 0.0018 0.003 0.0155 0.0034 0.003 0.001 0.001
Bud Site 0.0036 0.0085 0.0018 0.0004 0.0018 0.0004 0.0084 0.0577 0.0154 0.0015 0.0024 0.0014 0.0042 0.0188 0.017 0.0011 0.0006 0.0005
Cell Periphery 0.0005 0.0008 0.0004 0.0001 0.0003 0.0002 0.0029 0.0029 0.004 0.0044 0.0004 0.0012 0.0011 0.003 0.0019 0.0008 0.0002 0.0003
Cytoplasm 0.0016 0.0063 0.0059 0.0011 0.0035 0.0023 0.0119 0.0285 0.0247 0.0055 0.0046 0.0111 0.0088 0.0219 0.0074 0.0058 0.0116 0.0036
Cytoplasmic Foci 0.0216 0.0297 0.0239 0.013 0.0286 0.0236 0.0576 0.0367 0.0346 0.0351 0.0686 0.0403 0.0488 0.0675 0.0329 0.0265 0.0164 0.0174
Eisosomes 0.0007 0.0005 0.0003 0.0001 0.0002 0.0003 0.001 0.0008 0.0009 0.0009 0.0016 0.0008 0.0006 0.0006 0.002 0.0004 0.0001 0.0002
Endoplasmic Reticulum 0.0017 0.0005 0.0008 0.0012 0.0005 0.0018 0.0024 0.001 0.0006 0.0009 0.0019 0.0013 0.0037 0.0023 0.0021 0.0054 0.0006 0.0009
Endosome 0.0807 0.0705 0.0493 0.0754 0.0629 0.058 0.0836 0.0502 0.0351 0.0332 0.0761 0.0662 0.1356 0.111 0.0694 0.0601 0.0981 0.0562
Golgi 0.0587 0.0912 0.0369 0.0493 0.0441 0.0566 0.054 0.064 0.0317 0.0227 0.0959 0.0557 0.0754 0.0827 0.0542 0.0866 0.0742 0.0671
Lipid Particles 0.0288 0.0054 0.0081 0.0326 0.0091 0.0186 0.0326 0.0033 0.0073 0.0081 0.0094 0.0185 0.0438 0.0468 0.0151 0.0068 0.0083 0.0127
Mitochondria 0.7125 0.7262 0.795 0.8096 0.7342 0.7834 0.6188 0.6115 0.7338 0.7772 0.546 0.7077 0.5553 0.5343 0.709 0.7094 0.6673 0.7867
None 0.0066 0.0025 0.0107 0.0002 0.0007 0.0006 0.0362 0.0243 0.0234 0.0124 0.0368 0.0202 0.0154 0.0038 0.0057 0.0015 0.0655 0.013
Nuclear Periphery 0.001 0.0001 0.0004 0.0003 0.0003 0.0005 0.0011 0.0002 0.0019 0.0002 0.0036 0.0005 0.0068 0.0011 0.0016 0.0009 0.0003 0.0002
Nucleolus 0.0009 0.0003 0.0003 0.0001 0.0002 0.0004 0.0015 0.0005 0.0035 0.0006 0.0003 0.0002 0.0009 0.001 0.0006 0.0001 0.0002 0.0002
Nucleus 0.0007 0.0003 0.0003 0.0001 0.0004 0.0007 0.0012 0.0006 0.0018 0.0004 0.0005 0.0004 0.0017 0.001 0.0007 0.0005 0.0003 0.0002
Peroxisomes 0.0359 0.0324 0.0383 0.0081 0.082 0.0294 0.0377 0.0526 0.05 0.0657 0.0962 0.051 0.0363 0.0365 0.0329 0.0484 0.0213 0.0246
Punctate Nuclear 0.0006 0.0001 0.0001 0.0001 0.0001 0.0005 0.0054 0.0001 0.0051 0.0003 0.0026 0.0002 0.0024 0.0004 0.0007 0.0002 0.0001 0.0003
Vacuole 0.0098 0.0054 0.004 0.0017 0.0054 0.004 0.0127 0.0052 0.0061 0.0037 0.0013 0.0034 0.0139 0.0286 0.0127 0.005 0.0127 0.0048
Vacuole Periphery 0.0052 0.0035 0.0039 0.0024 0.0044 0.0037 0.0039 0.0028 0.0028 0.0027 0.0014 0.0024 0.0077 0.0093 0.0095 0.0037 0.0162 0.0037

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 8.2568 13.4201 5.975 12.8803 4.866 3.0008 13.569 12.8215 16.2537 14.2067
Translational Efficiency 1.8913 0.7921 2.0749 1.0389 2.838 6.1797 1.0997 0.9117 1.0789 0.942

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1283 590 2172 1623 1410 1841 946 1561 2693 2431 3118 3184

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 562.98 815.38 787.81 830.87 600.51 786.63 879.80 831.48 582.63 793.61 815.72 831.17
Standard Deviation 79.65 116.49 92.16 109.94 75.85 120.98 115.05 148.54 79.91 120.54 108.27 130.30
Intensity Change Log 2 — 0.534389 0.484764 0.561539 — 0.389497 0.550987 0.469493 — 0.461424 0.519323 0.514765

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000462 0.003177 0.002404 0.005575 0.000361 0.001190 0.006472 0.002724 0.000409 0.001672 0.003639 0.004177
Bud Neck 0.027643 0.010868 0.035649 0.028879 0.016206 0.020120 0.019178 0.023421 0.021655 0.017875 0.030652 0.026203
Bud Site 0.017026 0.024445 0.085729 0.082755 0.013156 0.019053 0.073005 0.075704 0.015000 0.020361 0.081868 0.079298
Cell Periphery 0.002108 0.000431 0.000281 0.000287 0.000443 0.000410 0.000280 0.000348 0.001236 0.000415 0.000281 0.000317
Cytoplasm 0.015577 0.006480 0.029738 0.059545 0.018459 0.019020 0.047200 0.045393 0.017086 0.015977 0.035036 0.052607
Cytoplasmic Foci 0.057093 0.071087 0.045282 0.116970 0.074563 0.087506 0.158379 0.103499 0.066240 0.083521 0.079596 0.110366
Eisosomes 0.000203 0.000109 0.000083 0.000141 0.000102 0.000147 0.000150 0.000110 0.000150 0.000138 0.000103 0.000126
Endoplasmic Reticulum 0.001309 0.001116 0.001499 0.002900 0.001029 0.000685 0.005737 0.001888 0.001162 0.000789 0.002785 0.002404
Endosome 0.035162 0.122046 0.068308 0.086719 0.054293 0.091931 0.105397 0.106069 0.045178 0.099240 0.079561 0.096205
Golgi 0.059334 0.151667* 0.036606 0.068817 0.078914 0.128571* 0.081755 0.085661 0.069586 0.134176* 0.050304 0.077075
Lipid Particles 0.015864 0.002312 0.000903 0.005443 0.011886 0.008252 0.006646 0.003300 0.013781 0.006810 0.002645 0.004392
Mitochondria 0.694914* 0.477273* 0.437683* 0.206788* 0.632225* 0.532661* 0.150456* 0.282914* 0.662091* 0.519219* 0.350539* 0.244109*
Mitotic Spindle 0.002732 0.021399 0.085129 0.065406 0.010645 0.007989 0.104213* 0.076684 0.006875 0.011243 0.090919 0.070935
None 0.004691 0.000359 0.000483 0.001552 0.002599 0.001433 0.001082 0.002323 0.003595 0.001172 0.000665 0.001930
Nuclear Periphery 0.000346 0.000523 0.000448 0.001516 0.000406 0.000144 0.000918 0.000449 0.000377 0.000236 0.000590 0.000993
Nuclear Periphery Foci 0.000613 0.000301 0.000310 0.000965 0.000404 0.000163 0.001603 0.000920 0.000503 0.000196 0.000702 0.000943
Nucleolus 0.003005 0.000514 0.001481 0.002635 0.004451 0.001514 0.002677 0.002002 0.003762 0.001272 0.001844 0.002325
Nucleus 0.006033 0.001692 0.010216 0.016127 0.009251 0.004830 0.017740 0.008342 0.007718 0.004068 0.012499 0.012311
Peroxisomes 0.029996 0.049382 0.030692 0.061532 0.030091 0.034593 0.081209 0.055602 0.030046 0.038183 0.046019 0.058625
Vacuole 0.011526 0.030140 0.104588 0.155708 0.017456 0.024414 0.111556 0.092866 0.014631 0.025803 0.106702 0.124899
Vacuole Periphery 0.014365 0.024680 0.022489 0.029739 0.023061 0.015374 0.024346 0.029781 0.018918 0.017633 0.023053 0.029759

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.56 -4.92 -7.42 -2.27 -3.91 -3.64 -7.96 -7.94 -4.27 4.64 -5.02 -8.93 -9.91 -5.51 -0.86
Bud Neck 5.30 -2.86 -0.49 -6.77 2.78 -1.66 -1.53 -3.29 -1.59 -2.05 1.82 -4.85 -2.56 -4.63 2.53
Bud Site -2.33 -19.47 -15.95 -12.19 0.88 -2.94 -14.76 -16.53 -14.49 -0.59 -3.16 -25.04 -23.02 -19.95 0.85
Cell Periphery 4.77 5.38 5.38 1.68 0.01 0.47 3.24 1.70 1.02 -1.78 4.81 5.80 5.59 2.11 -1.37
Cytoplasm 4.90 -6.86 -13.72 -17.56 -9.14 -0.25 -8.41 -8.54 -8.84 0.54 0.59 -10.12 -15.79 -16.47 -7.25
Cytoplasmic Foci -2.67 3.41 -12.65 -8.30 -18.40 -2.95 -14.17 -6.24 -3.71 9.02 -5.23 -3.93 -13.18 -7.73 -9.94
Eisosomes 4.87 6.59 3.20 -2.74 -6.29 -3.85 -4.91 -1.14 3.52 4.89 1.02 4.66 2.30 1.39 -3.83
Endoplasmic Reticulum 0.38 -1.25 -4.54 -3.67 -4.18 1.68 -7.21 -2.64 -3.94 5.64 1.94 -6.83 -5.21 -6.76 1.72
Endosome -12.50 -10.04 -13.45 5.24 -4.12 -8.73 -9.38 -10.92 -2.61 -0.53 -15.67 -11.62 -16.53 1.38 -5.00
Golgi -10.90 6.19 -1.19 10.38 -8.23 -8.94 0.25 -0.96 8.17 -1.16 -14.45 6.94 -1.33 13.68 -8.82
Lipid Particles 6.63 7.53 4.90 -3.56 -6.15 2.29 3.15 6.50 4.58 3.09 5.04 9.26 7.64 2.70 -3.27
Mitochondria 13.05 22.84 44.46* 18.17 23.71* 8.23 41.73* 29.66* 23.01 -12.55 15.09 36.17 51.67* 32.92 13.43
Mitotic Spindle -5.59 -23.51* -15.65 -8.54 3.70 2.06 -14.38 -15.05 -15.92 3.76 -3.81 -26.48 -21.55 -19.43 4.80
None 3.77 3.68 2.49 -1.92 -1.75 1.82 2.54 0.33 -0.70 -1.06 3.48 4.90 2.14 -0.97 -2.08
Nuclear Periphery -0.57 -1.02 -2.56 -1.72 -2.27 2.35 -2.26 -0.48 -5.02 2.19 1.18 -2.09 -2.59 -3.06 -1.55
Nuclear Periphery Foci 0.82 0.74 -1.09 -3.77 -3.87 3.38 -5.67 -2.47 -3.71 2.62 1.77 -1.50 -2.27 -5.80 -1.33
Nucleolus 3.39 2.00 0.47 -4.48 -2.26 3.63 1.73 2.78 -1.30 1.34 4.46 3.19 2.36 -3.20 -1.19
Nucleus 3.36 -4.00 -6.38 -9.49 -3.18 2.71 -4.26 0.01 -3.07 4.46 3.28 -5.12 -4.60 -8.25 0.50
Peroxisomes -3.34 -0.27 -7.22 -1.65 -7.60 -1.51 -9.47 -6.57 -5.51 4.32 -3.17 -6.23 -9.79 -6.37 -3.77
Vacuole -6.56 -26.94 -27.70 -22.75 -9.26 -3.71 -17.64 -19.51 -17.36 3.54 -7.26 -32.05 -33.27 -29.13 -4.59
Vacuole Periphery -4.01 -5.32 -7.28 -1.21 -3.16 4.72 -0.08 -2.72 -7.06 -2.45 1.04 -2.80 -6.55 -7.54 -4.18
Standard name
Human Ortholog
Description Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
Localization
Cell Percentages mitochondrion (82%)
Cell Cycle Regulation No
Subcompartmental Group mito-1

Fmp10

Fmp10


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Fmp10-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available