Standard name | |
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Human Ortholog | |
Description | 5,10-methenyltetrahydrofolate synthetase; involved in folic acid biosynthesis |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 1 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 3 | 1 | 5 | 8 | 13 |
Bud | 2 | 2 | 0 | 1 | 0 | 0 | 1 | – | 4 | 1 | 0 | 3 | 0 | 2 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 2 | 8 | 10 |
Bud Neck | 0 | 4 | 0 | 1 | 4 | 0 | 3 | – | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 1 | 1 | 0 | 3 | 3 | 4 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 1 | 0 | 4 | 1 | 0 | 2 | – | 0 | 2 | 0 | 0 | 2 | 3 | 2 | 0 | 2 | 1 | 1 | 0 | 0 | 3 | 1 | 4 |
Cytoplasm | 259 | 185 | 24 | 167 | 210 | 107 | 199 | – | 57 | 92 | 33 | 75 | 227 | 428 | 466 | 127 | 90 | 105 | 202 | 151 | 53 | 188 | 256 | 259 |
Endoplasmic Reticulum | 0 | 1 | 0 | 0 | 0 | 0 | 0 | – | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 9 | 8 | 11 | 4 | 1 | 1 | 3 | 1 | 3 |
Endosome | 4 | 0 | 0 | 0 | 4 | 1 | 6 | – | 0 | 1 | 1 | 0 | 0 | 2 | 1 | 2 | 1 | 1 | 7 | 4 | 0 | 8 | 8 | 4 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 1 | 1 |
Mitochondria | 9 | 16 | 0 | 5 | 1 | 31 | 24 | – | 31 | 45 | 27 | 71 | 1 | 1 | 0 | 10 | 4 | 3 | 63 | 30 | 13 | 60 | 81 | 67 |
Nucleus | 0 | 1 | 0 | 1 | 1 | 3 | 4 | – | 5 | 3 | 2 | 3 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 1 | 2 |
Nuclear Periphery | 0 | 1 | 0 | 0 | 0 | 1 | 2 | – | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 |
Nucleolus | 0 | 1 | 0 | 0 | 0 | 1 | 0 | – | 3 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 1 | 0 | 0 | 1 | – | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
SpindlePole | 1 | 2 | 0 | 5 | 1 | 0 | 15 | – | 1 | 4 | 3 | 4 | 0 | 0 | 1 | 0 | 0 | 0 | 4 | 3 | 0 | 5 | 10 | 7 |
Vac/Vac Membrane | 8 | 1 | 0 | 2 | 6 | 2 | 12 | – | 3 | 1 | 1 | 1 | 2 | 1 | 0 | 2 | 1 | 1 | 3 | 3 | 0 | 3 | 8 | 6 |
Unique Cell Count | 272 | 202 | 24 | 176 | 217 | 127 | 242 | 86 | 125 | 59 | 130 | 232 | 435 | 468 | 135 | 96 | 112 | 299 | 215 | 75 | 292 | 403 | 395 | |
Labelled Cell Count | 283 | 216 | 24 | 187 | 228 | 146 | 269 | 106 | 150 | 70 | 158 | 234 | 441 | 471 | 153 | 108 | 122 | 299 | 215 | 75 | 292 | 403 | 395 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 3.9 | 3.9 | 5.4 | 6.2 | 5.1 | 3.8 | 4.5 | – | 4.3 | 4.3 | 4.5 | 3.8 | 4.8 | 4.9 | 4.9 | 4.9 | 5.0 | 5.1 | 4.1 | 4.4 | 4.6 |
Std Deviation (1e-4) | 0.5 | 1.1 | 3.2 | 6.7 | 1.4 | 1.7 | 2.0 | – | 2.3 | 2.4 | 2.4 | 1.8 | 1.7 | 1.6 | 1.7 | 0.8 | 0.7 | 1.2 | 1.3 | 1.9 | 2.0 |
Intensity Change (Log2) | – | – | – | 0.2 | -0.07 | -0.51 | -0.26 | – | -0.32 | -0.32 | -0.25 | -0.49 | -0.16 | -0.13 | -0.12 | -0.13 | -0.1 | -0.08 | -0.38 | -0.28 | -0.23 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | -1.9355 | -1.507 | -1.6744 | -1.7195 | -1.6156 | -1.4682 | 0.0297 | -0.2872 | -0.0563 | -0.7479 | -0.552 | -0.3469 | 1.04 | 1.5005 | 1.4734 | 1.8171 | 1.274 | 1.5698 |
Actin | 0.0056 | 0.0004 | 0.0022 | 0.0008 | 0.0279 | 0.0008 | 0.0311 | 0.0111 | 0.0181 | 0.0011 | 0.0019 | 0.0022 | 0.0244 | 0.0075 | 0.0136 | 0.0133 | 0.0228 | 0.0054 |
Bud | 0.002 | 0.0009 | 0.0007 | 0.0001 | 0.0006 | 0.0013 | 0.001 | 0.0012 | 0.001 | 0.0003 | 0.0003 | 0.0013 | 0.0011 | 0.0001 | 0.0024 | 0.001 | 0.0011 | 0.0004 |
Bud Neck | 0.0005 | 0.0002 | 0.0012 | 0.0006 | 0.0006 | 0.0007 | 0.0026 | 0.0008 | 0.0023 | 0.0006 | 0.0007 | 0.0028 | 0.0003 | 0.0001 | 0.0008 | 0.0005 | 0.0017 | 0.002 |
Bud Periphery | 0.0009 | 0.0008 | 0.0008 | 0.0002 | 0.0012 | 0.0005 | 0.0016 | 0.0016 | 0.002 | 0.0003 | 0.0014 | 0.0033 | 0.0032 | 0.0001 | 0.0036 | 0.0019 | 0.0031 | 0.0009 |
Bud Site | 0.0014 | 0.012 | 0.0071 | 0.0002 | 0.0011 | 0.0017 | 0.01 | 0.0188 | 0.0073 | 0.0007 | 0.0006 | 0.0006 | 0.001 | 0.0011 | 0.0032 | 0.0039 | 0.0031 | 0.0021 |
Cell Periphery | 0.0003 | 0.0005 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0005 | 0.0004 | 0.0004 | 0.0001 | 0.0005 | 0.0002 | 0.0004 | 0.0001 | 0.0004 | 0.0003 | 0.0004 | 0.0002 |
Cytoplasm | 0.0861 | 0.0995 | 0.083 | 0.0533 | 0.1555 | 0.1307 | 0.1954 | 0.3138 | 0.2688 | 0.4199 | 0.3501 | 0.319 | 0.0903 | 0.2018 | 0.1256 | 0.1341 | 0.1173 | 0.2336 |
Cytoplasmic Foci | 0.0419 | 0.04 | 0.0312 | 0.0425 | 0.0327 | 0.039 | 0.0521 | 0.0402 | 0.0391 | 0.0309 | 0.0501 | 0.0334 | 0.0299 | 0.0201 | 0.0247 | 0.036 | 0.0562 | 0.0277 |
Eisosomes | 0.0008 | 0.0001 | 0.0005 | 0.0004 | 0.0002 | 0.0002 | 0.0007 | 0.0003 | 0.0004 | 0.0001 | 0.0003 | 0.0001 | 0.0005 | 0.0001 | 0.0004 | 0.0004 | 0.0007 | 0.0001 |
Endoplasmic Reticulum | 0.003 | 0.0022 | 0.0015 | 0.0007 | 0.0017 | 0.0011 | 0.0112 | 0.0028 | 0.0044 | 0.0031 | 0.0023 | 0.005 | 0.0025 | 0.0005 | 0.0022 | 0.0023 | 0.0015 | 0.0013 |
Endosome | 0.0142 | 0.0211 | 0.0062 | 0.0226 | 0.0267 | 0.0127 | 0.058 | 0.0161 | 0.0269 | 0.0186 | 0.0418 | 0.0188 | 0.0134 | 0.0025 | 0.0123 | 0.0201 | 0.0126 | 0.0082 |
Golgi | 0.0017 | 0.0016 | 0.0006 | 0.0005 | 0.003 | 0.0005 | 0.0092 | 0.0034 | 0.01 | 0.0032 | 0.0039 | 0.0049 | 0.0038 | 0.0016 | 0.0048 | 0.0054 | 0.0028 | 0.0032 |
Lipid Particles | 0.0096 | 0.0037 | 0.0021 | 0.0015 | 0.0092 | 0.0023 | 0.0274 | 0.0024 | 0.0118 | 0.0004 | 0.0088 | 0.0061 | 0.0073 | 0.0025 | 0.0023 | 0.0018 | 0.0053 | 0.0037 |
Mitochondria | 0.0022 | 0.0035 | 0.0016 | 0.0013 | 0.0193 | 0.0018 | 0.017 | 0.0048 | 0.0178 | 0.0015 | 0.009 | 0.0077 | 0.0062 | 0.0005 | 0.0032 | 0.0071 | 0.0059 | 0.0071 |
None | 0.8043 | 0.8059 | 0.8456 | 0.8673 | 0.691 | 0.793 | 0.5133 | 0.5722 | 0.5673 | 0.5125 | 0.4987 | 0.5677 | 0.794 | 0.7497 | 0.7807 | 0.7643 | 0.7433 | 0.6951 |
Nuclear Periphery | 0.0021 | 0.001 | 0.0013 | 0.0015 | 0.0087 | 0.0017 | 0.0213 | 0.0009 | 0.0022 | 0.0007 | 0.009 | 0.0064 | 0.0041 | 0.0002 | 0.0011 | 0.0009 | 0.0009 | 0.0009 |
Nucleolus | 0.0013 | 0.0004 | 0.0007 | 0.0003 | 0.001 | 0.0005 | 0.002 | 0.0004 | 0.0006 | 0.0004 | 0.0037 | 0.0008 | 0.0004 | 0.0005 | 0.0003 | 0.0003 | 0.0005 | 0.0004 |
Nucleus | 0.0027 | 0.0011 | 0.0027 | 0.0007 | 0.0022 | 0.0027 | 0.007 | 0.0017 | 0.0016 | 0.0013 | 0.0061 | 0.0078 | 0.0029 | 0.0007 | 0.0011 | 0.0008 | 0.0009 | 0.0008 |
Peroxisomes | 0.0084 | 0.001 | 0.0023 | 0.0013 | 0.0057 | 0.0014 | 0.0154 | 0.002 | 0.0109 | 0.0005 | 0.0022 | 0.0045 | 0.0098 | 0.0079 | 0.0083 | 0.0033 | 0.0153 | 0.0034 |
Punctate Nuclear | 0.0089 | 0.0013 | 0.0066 | 0.0013 | 0.0043 | 0.0057 | 0.0131 | 0.0017 | 0.0026 | 0.0015 | 0.0018 | 0.0039 | 0.0018 | 0.0012 | 0.0019 | 0.0008 | 0.0032 | 0.0023 |
Vacuole | 0.0019 | 0.0022 | 0.0016 | 0.002 | 0.0051 | 0.0014 | 0.007 | 0.003 | 0.0032 | 0.0018 | 0.0037 | 0.0028 | 0.0021 | 0.001 | 0.0062 | 0.0011 | 0.001 | 0.0011 |
Vacuole Periphery | 0.0004 | 0.0005 | 0.0003 | 0.001 | 0.0022 | 0.0003 | 0.0031 | 0.0005 | 0.0013 | 0.0005 | 0.0032 | 0.0008 | 0.0006 | 0.0001 | 0.0008 | 0.0004 | 0.0003 | 0.0004 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 22.5539 | 15.5091 | 12.6592 | 21.7434 | 19.1198 | 13.8716 | 19.2095 | 21.1675 | 20.1649 | 27.5787 |
Translational Efficiency | 1.1343 | 1.2373 | 1.3276 | 0.9482 | 0.9724 | 1.9356 | 1.433 | 1.088 | 1.187 | 0.895 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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