Standard name
Human Ortholog
Description Protein of unknown function; YET3 null mutant decreases the level of secreted invertase; homolog of human BAP31 protein; protein abundance increases in response to DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0.06 0 0.07 0.06 0 – 0.14 0.08 0.12 0 0.05 0.05 0.11 – – 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – – – – – – –
Cell Periphery 0 0.07 0.05 0.05 0.09 0.06 0.05 0 – 0.06 0.07 0.09 0 0.12 0.24 0.49 – – 0 0 0 0 0 0
Cytoplasm 0.05 0.05 0.08 0.08 0.06 0.09 0.1 0.14 – 0.09 0.11 0.12 0 0.09 0 0.17 – – 0 0 0 0 0 0
Endoplasmic Reticulum 0.85 0.89 0.83 0.81 0.8 0.85 0.79 0.7 – 0.53 0.64 0.64 0.93 0.83 0.81 0.06 – – 0.93 0.94 0.97 0.8 0.88 0.86
Endosome 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Golgi 0 0 0.05 0.09 0 0 0 0 – 0 0 0 0 0 0 0.29 – – 0 0 0 0 0 0
Mitochondria 0 0 0.08 0.06 0 0 0.06 0.07 – 0.08 0.18 0.13 0 0 0 0.14 – – 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Nuclear Periphery 0.08 0 0.11 0.11 0 0 0.06 0.05 – 0 0.08 0 0 0 0 0 – – 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Vac/Vac Membrane 0.16 0.11 0.1 0.07 0.1 0 0.07 0.07 – 0.26 0.09 0.08 0 0 0 0 – – 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 1 3 15 13 24 27 17 – 60 27 44 7 9 12 4 – – 0 2 1 3 2 4
Bud 1 0 2 0 6 5 4 3 – 14 4 7 0 1 1 1 – – 0 1 0 1 1 1
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – – – – – – –
Cell Periphery 6 13 10 13 32 20 20 17 – 26 23 33 8 20 60 17 – – 0 0 0 0 0 1
Cytoplasm 12 8 15 20 23 32 43 55 – 36 35 44 8 15 7 6 – – 2 0 1 2 4 3
Endoplasmic Reticulum 225 155 162 195 287 290 338 270 – 220 211 246 186 137 206 2 – – 263 178 205 126 225 205
Endosome 0 0 0 0 0 0 0 0 – 0 0 0 0 0 1 0 – – 0 0 0 2 0 1
Golgi 10 2 9 22 10 4 5 4 – 12 8 5 4 1 6 10 – – 1 0 0 2 4 2
Mitochondria 1 1 16 15 16 14 27 27 – 33 58 50 0 0 0 5 – – 2 0 0 3 2 5
Nucleus 1 0 1 0 2 2 1 2 – 1 0 3 0 0 0 0 – – 0 0 0 1 0 0
Nuclear Periphery 20 6 21 26 5 8 25 21 – 6 27 14 9 4 3 0 – – 5 0 0 3 3 0
Nucleolus 0 0 0 0 0 1 1 2 – 1 1 1 0 0 0 0 – – 0 0 0 1 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Vac/Vac Membrane 42 19 20 17 34 12 29 29 – 108 29 31 6 7 8 1 – – 4 3 0 5 6 8
Unique Cell Count 264 175 196 240 357 343 430 388 415 330 382 200 165 253 35 284 189 213 157 256 239
Labelled Cell Count 319 205 259 323 428 412 520 447 517 423 478 228 194 304 46 284 189 213 157 256 239


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 21.7 23.0 16.0 16.0 19.3 19.0 17.5 18.0 – 17.9 17.8 18.0 19.8 21.7 24.0 33.8 – – 20.4 24.3 27.0
Std Deviation (1e-4) 4.1 4.5 3.7 3.9 4.9 4.5 4.4 4.7 – 5.0 5.4 4.8 3.9 5.3 5.1 9.7 – – 5.2 7.1 8.0
Intensity Change (Log2) – – – 0.0 0.27 0.24 0.13 0.17 – 0.16 0.15 0.16 0.3 0.44 0.58 1.08 – – 0.35 0.6 0.75


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 2.5 1.4 2.8 2.6 1.8 – 4.9 3.2 4.2 0 2.1 1.9 0 – –
Bud 0 0 0 0 0 – 1.7 0 0 0 0 0 0 – –
Bud Neck 0 0 0 0 0 – 0 0 0 0 0 0 0 – –
Bud Site 0 0 0 0 0 – 0 0 0 0 0 0 0 – –
Cell Periphery 0.1 1.6 0.4 -0.2 -0.4 – 0.6 0.9 1.5 -0.5 2.4 5.4 0 – –
Cytoplasm 0.3 -0.5 0.7 0.9 2.3 – 0.4 1.1 1.5 -1.6 0.5 -2.4 0 – –
Endoplasmic Reticulum -0.4 -0.7 0.6 -1.2 -3.4 – -7.1 -4.6 -4.6 3.2 0.1 -0.3 -9.2 – –
Endosome 0 0 0 0 0 – 0 0 0 0 0 0 0 – –
Golgi 1.8 -1.1 0 0 0 – -1.1 -1.4 0 -1.4 0 -1.3 0 – –
Mitochondria -0.8 -1.8 -2.0 -0.9 -0.5 – -0.1 3.0 1.8 -4.1 -3.8 -4.6 0 – –
Nucleus 0 0 0 0 0 – 0 0 0 0 0 0 0 – –
Nuclear Periphery 0 -4.9 -4.1 -2.2 -2.3 – -5.2 -1.0 -3.4 -2.3 -3.1 -4.5 0 – –
Nucleolus 0 0 0 0 0 – 0 0 0 0 0 0 0 – –
Peroxisomes 0 0 0 0 0 – 0 0 0 0 0 0 0 – –
SpindlePole 0 0 0 0 0 – 0 0 0 0 0 0 0 – –
Vacuole -1.2 -0.3 -3.2 -1.5 -1.1 – 4.5 -0.5 -0.8 -2.9 -2.1 -3.1 0 – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 23.1571 23.2039 22.73 20.7236 18.8115 22.8411 21.2339 22.4135 19.8786 19.0019 18.1124 19.4877 24.7593 25.5172 22.5176 21.403 21.3567 22.5011
Actin 0.0145 0.0001 0.0052 0.0157 0.0012 0 0.0271 0 0.0199 0.0066 0.0498 0.0107 0.0014 0.0002 0.0018 0 0 0.0023
Bud 0.0002 0 0.0001 0 0.0001 0 0.0001 0 0.0003 0.0003 0.0001 0.0001 0.0002 0.0001 0 0 0 0
Bud Neck 0.0006 0.0001 0 0.0002 0.0024 0.0001 0.0002 0 0.0003 0.0008 0.0003 0.0008 0.0014 0.0001 0.0002 0.0002 0 0.0007
Bud Periphery 0.0005 0 0.0002 0 0.0002 0 0.0002 0 0.0003 0.0005 0.0001 0.0003 0.0004 0.0001 0 0 0 0.0002
Bud Site 0.0021 0 0.0004 0.0001 0.0003 0 0.0003 0 0.0025 0.0011 0.0006 0.0001 0.0012 0.0003 0.0001 0 0 0.0001
Cell Periphery 0.0055 0.0086 0.002 0.0009 0.0029 0.001 0.0016 0.0016 0.0008 0.0079 0.0004 0.0004 0.0039 0.0122 0.0023 0.0118 0.0019 0.0011
Cytoplasm 0.0031 0.0018 0.0021 0.0007 0.0061 0.0007 0.0063 0.0006 0.0021 0.0067 0.0124 0.0079 0.0008 0.0025 0.0004 0.0003 0.0002 0.0015
Cytoplasmic Foci 0.0069 0 0.0016 0.0006 0.0119 0 0.0092 0 0.003 0.0064 0.0092 0.0014 0.0035 0.0029 0.0003 0 0 0.0001
Eisosomes 0.001 0 0.0011 0.0001 0.0001 0 0.0003 0 0.0002 0.0001 0.0002 0.0001 0 0.0003 0.0002 0.002 0 0.0003
Endoplasmic Reticulum 0.8443 0.9711 0.9683 0.9579 0.8566 0.9825 0.8154 0.9819 0.9233 0.8709 0.8468 0.9231 0.9068 0.9513 0.9731 0.9615 0.9924 0.9691
Endosome 0.0102 0.0002 0.0031 0.0002 0.0118 0.0002 0.0149 0.0001 0.0107 0.0128 0.0402 0.0114 0.0018 0.0004 0.0015 0.0003 0.0002 0.0017
Golgi 0.003 0.0001 0.0009 0.0005 0.0027 0.0001 0.0102 0 0.0086 0.0041 0.005 0.0033 0.0009 0.0006 0.0047 0.0013 0.0003 0.001
Lipid Particles 0.009 0.0018 0.0036 0.0011 0.0143 0.0003 0.0179 0.0009 0.0077 0.0287 0.0095 0.0118 0.0022 0.0067 0.0009 0.0024 0.0002 0.0004
Mitochondria 0.0062 0 0.0032 0.0003 0.0185 0 0.0027 0 0.0026 0.0037 0.0009 0.0062 0.0007 0.0003 0.0004 0 0 0.0011
None 0.0096 0 0.0003 0 0.0046 0 0.0041 0 0.0004 0.0012 0.001 0.0002 0.0003 0.0002 0.0002 0.0002 0 0.0004
Nuclear Periphery 0.0611 0.0128 0.006 0.0057 0.0344 0.0135 0.064 0.0128 0.0077 0.0108 0.0094 0.0152 0.0633 0.0131 0.0088 0.0171 0.0038 0.0165
Nucleolus 0.0016 0 0 0 0.0009 0 0.0001 0 0.0001 0.0006 0.0004 0.0001 0.0001 0.0008 0.0002 0 0 0.0001
Nucleus 0.0038 0.0006 0.0002 0.0001 0.0195 0.0004 0.0081 0.0003 0.0008 0.0013 0.0023 0.0007 0.0012 0.001 0.0008 0.0001 0 0.001
Peroxisomes 0.004 0 0.0002 0.0152 0.0014 0 0.0058 0 0.0062 0.0006 0.0006 0.0018 0.0027 0.0001 0.0002 0 0 0
Punctate Nuclear 0.0032 0 0.0001 0 0.0034 0 0.0023 0 0.0005 0.0279 0.0017 0.0002 0.0012 0.0009 0.002 0 0 0.0001
Vacuole 0.0063 0.0025 0.0012 0.0006 0.0048 0.0009 0.0059 0.0014 0.0014 0.0063 0.0079 0.0025 0.0036 0.0047 0.0015 0.0018 0.0006 0.0017
Vacuole Periphery 0.0032 0.0002 0.0003 0.0001 0.002 0.0003 0.003 0.0002 0.0005 0.0008 0.0013 0.0017 0.0023 0.0009 0.0004 0.0007 0.0002 0.0006

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 135.2211 109.5974 95.6776 120.1884 100.5169 120.7307 119.7766 107.2383 119.1316 118.1877
Translational Efficiency 1.1434 1.2086 1.4204 1.0905 1.2047 1.5782 1.2611 1.3118 1.3117 1.201

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
174 1301 2254 1714 1887 1925 1831 1716 2061 3226 4085 3430

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1376.24 1676.86 2874.66 3889.48 1509.25 1505.43 2872.42 3652.06 1498.02 1574.57 2873.66 3770.70
Standard Deviation 280.75 337.59 480.81 758.52 267.67 299.65 521.90 767.85 271.33 326.52 499.65 772.38
Intensity Change Log 2 — 0.285030 1.062659 1.498845 — -0.003656 0.928435 1.274879 — 0.141249 0.994013 1.386043

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000394 0.000745 0.000191 0.001603 0.000115 0.000853 0.000167 0.000410 0.000138 0.000810 0.000180 0.001006
Bud Neck 0.000357 0.000360 0.000984 0.005383 0.000119 0.000456 0.001010 0.001656 0.000139 0.000417 0.000996 0.003519
Bud Site 0.000774 0.002504 0.000966 0.008517 0.000485 0.003561 0.000812 0.003918 0.000509 0.003135 0.000897 0.006216
Cell Periphery 0.002121 0.001451 0.002163 0.024797 0.000934 0.001839 0.004961 0.019992 0.001034 0.001682 0.003417 0.022393
Cytoplasm 0.000148 0.002073 0.002193 0.003947 0.001118 0.004083 0.000317 0.000618 0.001036 0.003273 0.001352 0.002281
Cytoplasmic Foci 0.000222 0.001050 0.000034 0.001460 0.000421 0.001281 0.000023 0.003088 0.000404 0.001188 0.000029 0.002274
Eisosomes 0.000055 0.000029 0.000020 0.000416 0.000005 0.000027 0.000212 0.000359 0.000009 0.000028 0.000106 0.000387
Endoplasmic Reticulum 0.966383* 0.970424* 0.967702* 0.842998* 0.982026* 0.956524* 0.976260* 0.920025* 0.980706* 0.962130* 0.971538* 0.881534*
Endosome 0.005427 0.006501 0.004009 0.010298 0.004572 0.007642 0.002757 0.004831 0.004644 0.007182 0.003448 0.007563
Golgi 0.000648 0.001693 0.000257 0.008933 0.000558 0.001739 0.001143 0.010717 0.000565 0.001720 0.000655 0.009825
Lipid Particles 0.000782 0.001578 0.002289 0.040149 0.001117 0.004123 0.001956 0.010543 0.001089 0.003096 0.002140 0.025337
Mitochondria 0.003361 0.000615 0.000260 0.001126 0.000162 0.000816 0.000179 0.000818 0.000432 0.000735 0.000224 0.000972
Mitotic Spindle 0.000150 0.000199 0.000035 0.001385 0.000018 0.000163 0.000128 0.000185 0.000029 0.000178 0.000077 0.000785
None 0.000030 0.000474 0.001040 0.001842 0.000320 0.001588 0.000222 0.000523 0.000295 0.001139 0.000673 0.001183
Nuclear Periphery 0.005936 0.002093 0.006947 0.007757 0.002113 0.003480 0.002966 0.004786 0.002436 0.002920 0.005163 0.006271
Nuclear Periphery Foci 0.007899 0.004775 0.007426 0.015567 0.004037 0.008275 0.001784 0.002286 0.004363 0.006863 0.004897 0.008923
Nucleolus 0.000047 0.000021 0.000005 0.000030 0.000004 0.000012 0.000004 0.000044 0.000007 0.000015 0.000005 0.000037
Nucleus 0.000347 0.000094 0.000243 0.000681 0.000046 0.000173 0.000070 0.000240 0.000071 0.000141 0.000166 0.000460
Peroxisomes 0.000055 0.000137 0.000006 0.000104 0.000025 0.000115 0.000006 0.000147 0.000028 0.000124 0.000006 0.000126
Vacuole 0.001834 0.002486 0.003007 0.019982 0.001335 0.002595 0.004445 0.010628 0.001377 0.002551 0.003651 0.015303
Vacuole Periphery 0.003032 0.000696 0.000221 0.003023 0.000471 0.000657 0.000576 0.004184 0.000687 0.000673 0.000380 0.003604

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.01 -0.01 -3.78 -2.84 -4.15 -2.03 -0.90 -2.55 1.01 -2.07 -2.73 -1.46 -5.57 -1.41 -4.47
Bud Neck -0.05 -3.40 -7.75 -7.77 -6.62 -3.53 -2.34 -4.15 -3.26 -1.07 -3.73 -4.41 -9.02 -8.35 -6.08
Bud Site -1.91 -1.09 -6.86 -5.00 -6.52 -3.70 -0.83 -3.96 -0.58 -3.39 -4.23 -1.81 -8.02 -4.25 -7.27
Cell Periphery 0.79 -0.36 -11.76 -13.10 -12.51 -2.28 -6.74 -12.73 -12.16 -9.63 -1.95 -6.42 -18.08 -17.78 -15.92
Cytoplasm -2.82 -7.27 -5.87 -2.51 -2.40 -4.79 4.25 0.64 4.66 -1.50 -4.68 -2.22 -3.76 0.87 -2.49
Cytoplasmic Foci -1.97 1.77 -3.61 -0.98 -4.25 -2.19 1.80 -2.44 -1.66 -2.85 -2.40 1.84 -3.20 -1.89 -4.02
Eisosomes 0.76 0.97 -5.58 -6.78 -6.98 -3.82 -3.24 -9.78 -9.06 -2.01 -3.11 -3.40 -11.30 -10.65 -6.34
Endoplasmic Reticulum -0.43 0.26 12.80 18.39 18.37 7.63 2.61 13.69 7.33 12.07 6.80 4.95 23.76 18.75 21.47
Endosome -0.88 1.05 -4.22 -5.36 -10.38 -5.20 4.16 -0.70 4.63 -5.00 -5.21 2.75 -6.77 -1.24 -11.08
Golgi -2.43 2.54 -11.96 -9.11 -12.72 -2.27 -2.46 -13.02 -10.19 -12.71 -3.01 -0.43 -17.01 -13.50 -18.05
Lipid Particles -1.92 -2.81 -13.18 -12.89 -12.47 -3.21 -1.53 -7.49 -4.49 -6.89 -3.07 -2.20 -14.50 -13.15 -13.93
Mitochondria 1.06 1.16 0.77 -1.73 -3.13 -3.09 -0.82 -3.71 -0.31 -3.56 -1.12 0.66 -2.44 -1.55 -4.50
Mitotic Spindle -0.33 1.20 -3.19 -3.02 -3.50 -2.60 -1.02 -5.51 -0.33 -0.51 -2.47 -1.06 -3.89 -3.07 -3.55
None -3.06 -3.45 -2.98 -2.20 -1.21 -2.45 0.76 -1.12 1.85 -1.64 -2.61 -1.99 -2.73 -0.19 -1.50
Nuclear Periphery 1.59 -0.48 -0.86 -8.58 -0.95 -2.48 -2.24 -6.07 -2.48 -4.76 -1.03 -5.05 -8.35 -7.90 -2.12
Nuclear Periphery Foci 1.05 -0.00 -2.72 -6.93 -4.99 -4.29 3.35 2.38 6.58 -1.13 -3.07 -1.08 -5.29 -2.72 -4.62
Nucleolus 0.67 1.13 0.23 -1.24 -5.43 -2.64 -0.94 -3.77 -2.98 -3.67 -1.28 0.63 -4.95 -3.22 -5.78
Nucleus 0.84 -0.27 -2.06 -5.10 -3.00 -4.59 -1.78 -2.93 -1.49 -2.51 -2.19 -3.32 -5.73 -5.13 -3.54
Peroxisomes -1.26 1.83 -0.81 0.43 -1.83 -3.05 3.04 -1.82 -0.45 -2.11 -3.19 3.43 -2.27 -0.02 -2.79
Vacuole -1.02 -2.85 -12.12 -11.57 -11.10 -2.77 -5.28 -10.16 -8.61 -6.07 -2.94 -6.05 -15.63 -14.30 -12.66
Vacuole Periphery 1.43 1.73 -0.09 -7.39 -9.98 -1.18 -0.86 -6.32 -5.96 -6.09 0.05 1.65 -8.22 -8.83 -9.94
Standard name
Human Ortholog
Description Protein of unknown function; YET3 null mutant decreases the level of secreted invertase; homolog of human BAP31 protein; protein abundance increases in response to DNA replication stress
Localization
Cell Percentages ER (99%)
Cell Cycle Regulation No
Subcompartmental Group ER-5

Yet3

Yet3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Yet3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available