Standard name | |
---|---|
Human Ortholog | |
Description | Subunit of the multiprotein cohesin complex; essential protein involved in chromosome segregation and in double-strand DNA break repair; SMC chromosomal ATPase family member, binds DNA with a preference for DNA with secondary structure |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 0 | 0 | 2 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 3 | 4 | 0 | 7 | 6 | 3 | 2 | 7 | 0 | 0 | 0 | 0 | 0 | 0 | 16 | 17 | 7 | 3 | 12 | 11 |
Bud Neck | 0 | 0 | 1 | 0 | 1 | 1 | 0 | 1 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 1 |
Bud Site | 0 | 0 | 1 | 1 | 2 | 6 | 12 | 9 | 15 | 17 | 8 | 17 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 30 | 17 | 22 | 19 | 41 | 30 | 65 | 39 | 28 | 41 | 26 | 27 | 12 | 29 | 33 | 24 | 34 | 18 | 14 | 5 | 12 | 7 | 18 | 36 |
Endoplasmic Reticulum | 0 | 1 | 1 | 0 | 0 | 0 | 2 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 8 | 7 | 5 | 0 | 0 | 1 | 0 | 1 | 1 |
Endosome | 19 | 7 | 7 | 1 | 25 | 2 | 4 | 2 | 2 | 1 | 0 | 1 | 13 | 12 | 4 | 8 | 6 | 5 | 11 | 5 | 3 | 2 | 5 | 10 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 1 | 12 | 44 | 75 | 46 | 101 | 170 | 197 | 153 | 228 | 116 | 167 | 4 | 1 | 12 | 2 | 3 | 2 | 2 | 5 | 5 | 0 | 1 | 2 |
Nucleus | 168 | 87 | 113 | 88 | 97 | 137 | 186 | 210 | 158 | 201 | 123 | 154 | 47 | 41 | 34 | 98 | 86 | 57 | 197 | 109 | 122 | 53 | 122 | 109 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 1 | 2 | 5 | 1 | 2 | 2 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 |
Nucleolus | 81 | 50 | 30 | 47 | 111 | 80 | 123 | 163 | 85 | 121 | 67 | 94 | 40 | 50 | 57 | 25 | 35 | 30 | 62 | 30 | 22 | 1 | 8 | 8 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 2 | 4 | 7 | 3 | 9 | 5 | 8 | 8 | 2 | 5 | 2 | 2 | 4 | 4 | 0 | 1 | 1 | 0 | 43 | 27 | 35 | 0 | 5 | 8 |
Vac/Vac Membrane | 92 | 45 | 33 | 17 | 32 | 32 | 35 | 28 | 11 | 16 | 13 | 19 | 30 | 34 | 32 | 57 | 43 | 34 | 15 | 16 | 22 | 4 | 32 | 49 |
Unique Cell Count | 339 | 184 | 207 | 181 | 286 | 297 | 459 | 503 | 325 | 438 | 251 | 345 | 128 | 141 | 145 | 195 | 196 | 138 | 372 | 223 | 243 | 76 | 213 | 247 |
Labelled Cell Count | 394 | 223 | 259 | 251 | 368 | 400 | 610 | 666 | 464 | 637 | 359 | 488 | 151 | 172 | 174 | 224 | 217 | 153 | 372 | 223 | 243 | 76 | 213 | 247 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 10.7 | 6.5 | 5.6 | 5.5 | 6.3 | 5.6 | 5.3 | 5.3 | 5.1 | 5.0 | 5.1 | 4.8 | 8.6 | 8.4 | 7.6 | 13.0 | 13.8 | 13.7 | 8.4 | 8.3 | 8.7 |
Std Deviation (1e-4) | 1.8 | 1.3 | 1.0 | 1.3 | 1.7 | 1.4 | 1.4 | 1.2 | 1.4 | 1.4 | 1.3 | 1.6 | 1.9 | 2.0 | 2.0 | 3.1 | 3.3 | 3.1 | 0.8 | 1.6 | 1.4 |
Intensity Change (Log2) | – | – | – | -0.03 | 0.18 | 0.0 | -0.07 | -0.08 | -0.14 | -0.17 | -0.14 | -0.21 | 0.62 | 0.59 | 0.45 | 1.21 | 1.3 | 1.29 | 0.58 | 0.57 | 0.64 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 2.7 | 2.4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 1.2 | -0.2 | 1.3 | -1.2 | -0.8 | -0.5 | -0.1 | -1.1 | -0.4 | 2.6 | 3.1 | 0.5 | 1.9 | 0.7 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 2.4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.5 | 2.1 | 0 | 0.4 | -0.2 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 4.3 | -1.5 | 3.1 | 4.0 | 4.6 | 6.0 | 7.4 | 5.6 | 0 | -4.6 | -5.6 | -3.3 | -6.4 | -6.2 | -5.3 |
Nucleus | -1.2 | -4.6 | -1.9 | -3.4 | -3.1 | -1.3 | -2.1 | -1.2 | -2.3 | -3.2 | -4.7 | -5.8 | -0.9 | -2.1 | -2.4 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 2.8 | 5.9 | 3.3 | 3.5 | 4.9 | 3.2 | 3.7 | 3.2 | 0 | 3.7 | 4.6 | 5.3 | -0.5 | 0.9 | 1.7 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | -0.1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -1.9 | -1.5 | -1.7 | -3.3 | -4.5 | -5.1 | -5.5 | -3.8 | -4.1 | 1.7 | 1.9 | 1.5 | 3.2 | 1.5 | 2.0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 5.0794 | 7.4814 | 7.0834 | 6.5921 | 5.4614 | 6.2397 | 5.9481 | 7.4915 | 7.5046 | 6.8542 | 6.0522 | 6.7109 | 6.771 | 8.4089 | 8.1617 | 7.5565 | 7.105 | 7.4575 |
Actin | 0.0003 | 0.001 | 0.0002 | 0.0003 | 0 | 0.0002 | 0.0218 | 0.0045 | 0.0092 | 0.0005 | 0.0853 | 0.0017 | 0.0036 | 0.0002 | 0.0022 | 0.0013 | 0.0001 | 0.0001 |
Bud | 0.0003 | 0.0005 | 0.0002 | 0.0005 | 0 | 0.0001 | 0.0013 | 0.0022 | 0.0002 | 0.0004 | 0.0004 | 0.0002 | 0.0002 | 0 | 0.0001 | 0.0003 | 0.0001 | 0.0001 |
Bud Neck | 0.0008 | 0.0007 | 0.0014 | 0.0016 | 0 | 0.0025 | 0.002 | 0.0008 | 0.0006 | 0.0013 | 0.0007 | 0.0027 | 0.0015 | 0.0004 | 0.0006 | 0.0011 | 0.0022 | 0.0022 |
Bud Periphery | 0.0002 | 0.0005 | 0.0002 | 0.0004 | 0 | 0.0001 | 0.0013 | 0.0008 | 0.0002 | 0.0008 | 0.0004 | 0.0002 | 0.0005 | 0 | 0.0001 | 0.0001 | 0.0002 | 0.0001 |
Bud Site | 0.0007 | 0.0065 | 0.0013 | 0.0023 | 0 | 0.0002 | 0.0096 | 0.0082 | 0.0008 | 0.0007 | 0.0008 | 0.0002 | 0.0005 | 0.0004 | 0.0006 | 0.0006 | 0.0011 | 0.0001 |
Cell Periphery | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0003 | 0.0001 | 0 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 | 0.0003 | 0 |
Cytoplasm | 0.0092 | 0.0068 | 0.0018 | 0.0012 | 0.0002 | 0.0023 | 0.0314 | 0.0406 | 0.0039 | 0.0005 | 0.0036 | 0.0007 | 0.0023 | 0.0116 | 0.001 | 0.0019 | 0.0029 | 0.0008 |
Cytoplasmic Foci | 0.0011 | 0.0015 | 0.0013 | 0.0025 | 0 | 0.0001 | 0.0351 | 0.0022 | 0.0008 | 0.0036 | 0.0275 | 0.0013 | 0.0006 | 0.0001 | 0.0005 | 0.0017 | 0.0015 | 0.0001 |
Eisosomes | 0 | 0 | 0 | 0 | 0 | 0 | 0.0002 | 0 | 0.0001 | 0 | 0.0002 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0009 | 0.0004 | 0.0001 | 0.0001 | 0 | 0.0003 | 0.011 | 0.0003 | 0.0008 | 0.0006 | 0.0059 | 0.0002 | 0.0004 | 0.0002 | 0.0002 | 0.0002 | 0.0002 | 0.0001 |
Endosome | 0.0018 | 0.0004 | 0.0006 | 0.0011 | 0 | 0.0005 | 0.0396 | 0.0004 | 0.0022 | 0.0305 | 0.112 | 0.0044 | 0.0009 | 0.0002 | 0.0008 | 0.0004 | 0.003 | 0.0004 |
Golgi | 0.0001 | 0.0001 | 0.0001 | 0.0004 | 0 | 0 | 0.0174 | 0.0001 | 0.0013 | 0.024 | 0.1036 | 0.005 | 0.0005 | 0 | 0.0003 | 0.0001 | 0.0007 | 0 |
Lipid Particles | 0.0002 | 0.0001 | 0.0002 | 0.0005 | 0 | 0 | 0.0225 | 0.0001 | 0.0007 | 0.0014 | 0.0096 | 0.0007 | 0.0015 | 0 | 0.0015 | 0.0001 | 0.0071 | 0.0001 |
Mitochondria | 0.001 | 0.0006 | 0.0003 | 0.0007 | 0.0001 | 0.0002 | 0.0114 | 0.0004 | 0.001 | 0.0019 | 0.0143 | 0.0007 | 0.0005 | 0.0001 | 0.0003 | 0.0001 | 0.0014 | 0.0001 |
None | 0.0006 | 0.0003 | 0.0004 | 0.0007 | 0 | 0.0002 | 0.0103 | 0.0009 | 0.0005 | 0.0001 | 0.0006 | 0.0002 | 0.0012 | 0.0002 | 0.0005 | 0.0003 | 0.0001 | 0.0002 |
Nuclear Periphery | 0.0269 | 0.0142 | 0.0044 | 0.0034 | 0.0006 | 0.0066 | 0.0292 | 0.004 | 0.0076 | 0.0012 | 0.0056 | 0.0025 | 0.0063 | 0.0056 | 0.0047 | 0.0039 | 0.0031 | 0.0031 |
Nucleolus | 0.0479 | 0.0452 | 0.1314 | 0.113 | 0.0095 | 0.0265 | 0.0249 | 0.0436 | 0.0636 | 0.0894 | 0.0448 | 0.0469 | 0.0433 | 0.0434 | 0.0601 | 0.0374 | 0.015 | 0.0725 |
Nucleus | 0.8489 | 0.8876 | 0.6776 | 0.6694 | 0.9894 | 0.9396 | 0.6657 | 0.8226 | 0.7819 | 0.7573 | 0.5755 | 0.9131 | 0.8794 | 0.9003 | 0.7777 | 0.7707 | 0.9293 | 0.8982 |
Peroxisomes | 0.0001 | 0 | 0 | 0.0002 | 0 | 0 | 0.0143 | 0.0003 | 0.0004 | 0.0002 | 0.0044 | 0.0001 | 0.0007 | 0 | 0.0007 | 0.0003 | 0.001 | 0 |
Punctate Nuclear | 0.0567 | 0.032 | 0.1777 | 0.2006 | 0.0001 | 0.0198 | 0.0439 | 0.0676 | 0.1232 | 0.0845 | 0.0025 | 0.0186 | 0.0553 | 0.0372 | 0.1477 | 0.1793 | 0.0274 | 0.0217 |
Vacuole | 0.0015 | 0.0008 | 0.0007 | 0.0006 | 0.0001 | 0.0005 | 0.0049 | 0.0003 | 0.0007 | 0.0005 | 0.001 | 0.0004 | 0.0004 | 0.0001 | 0.0001 | 0.0002 | 0.0027 | 0.0002 |
Vacuole Periphery | 0.0007 | 0.0004 | 0.0001 | 0.0003 | 0 | 0.0001 | 0.002 | 0.0001 | 0.0003 | 0.0005 | 0.0011 | 0.0002 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0004 | 0.0001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 46.6502 | 20.6501 | 15.9203 | 27.195 | 39.4799 | 35.9802 | 16.8797 | 16.2883 | 22.7366 | 43.7815 |
Translational Efficiency | 1.0886 | 0.8759 | 0.801 | 0.8931 | 0.9978 | 1.3615 | 0.8786 | 0.5928 | 0.7003 | 0.8486 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✔ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1788 | 89 | 632 | 58 | 2252 | 1157 | 1082 | 26 | 4040 | 1246 | 1714 | 84 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 740.86 | 1015.61 | 1178.28 | 983.03 | 945.74 | 878.23 | 1148.05 | 1095.39 | 855.07 | 888.04 | 1159.20 | 1017.81 |
Standard Deviation | 104.55 | 156.09 | 172.23 | 126.46 | 127.93 | 142.23 | 164.84 | 179.76 | 155.94 | 147.57 | 168.24 | 154.09 |
Intensity Change Log 2 | — | 0.455074 | 0.669410 | 0.408035 | — | -0.106845 | 0.279670 | 0.211929 | — | 0.167197 | 0.463938 | 0.301369 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000166 | 0.001931 | 0.001025 | 0.001000 | 0.000529 | 0.000734 | 0.000754 | 0.003224 | 0.000368 | 0.000819 | 0.000853 | 0.001688 |
Bud Neck | 0.012466 | 0.004480 | 0.016921 | 0.004629 | 0.019601 | 0.012576 | 0.018441 | 0.009423 | 0.016443 | 0.011998 | 0.017881 | 0.006113 |
Bud Site | 0.006522 | 0.001947 | 0.004589 | 0.001823 | 0.003202 | 0.006592 | 0.003324 | 0.004989 | 0.004671 | 0.006260 | 0.003791 | 0.002803 |
Cell Periphery | 0.000328 | 0.000173 | 0.000139 | 0.000174 | 0.000138 | 0.000269 | 0.000212 | 0.000662 | 0.000222 | 0.000262 | 0.000185 | 0.000326 |
Cytoplasm | 0.255899* | 0.016745 | 0.017555 | 0.018921 | 0.026437 | 0.068234 | 0.020578 | 0.019528 | 0.127991* | 0.064556 | 0.019464 | 0.019109 |
Cytoplasmic Foci | 0.028750 | 0.001877 | 0.001392 | 0.003996 | 0.001242 | 0.002741 | 0.000551 | 0.000443 | 0.013416 | 0.002679 | 0.000861 | 0.002897 |
Eisosomes | 0.000038 | 0.000074 | 0.000025 | 0.000064 | 0.000026 | 0.000034 | 0.000041 | 0.000076 | 0.000031 | 0.000037 | 0.000035 | 0.000068 |
Endoplasmic Reticulum | 0.001081 | 0.009008 | 0.003350 | 0.005108 | 0.002559 | 0.003609 | 0.005515 | 0.006985 | 0.001905 | 0.003995 | 0.004717 | 0.005689 |
Endosome | 0.001933 | 0.001449 | 0.000663 | 0.000742 | 0.000969 | 0.001318 | 0.000536 | 0.001356 | 0.001396 | 0.001327 | 0.000583 | 0.000932 |
Golgi | 0.000316 | 0.000640 | 0.000273 | 0.000198 | 0.000333 | 0.000624 | 0.000139 | 0.000498 | 0.000325 | 0.000625 | 0.000188 | 0.000291 |
Lipid Particles | 0.000876 | 0.001766 | 0.000285 | 0.001946 | 0.000462 | 0.001005 | 0.000163 | 0.000390 | 0.000645 | 0.001060 | 0.000208 | 0.001465 |
Mitochondria | 0.000768 | 0.019873 | 0.001712 | 0.001281 | 0.001834 | 0.003538 | 0.001201 | 0.002844 | 0.001362 | 0.004705 | 0.001389 | 0.001765 |
Mitotic Spindle | 0.001861 | 0.013725 | 0.025351 | 0.064435 | 0.016963 | 0.023667 | 0.007508 | 0.045944 | 0.010279 | 0.022957 | 0.014088 | 0.058712 |
None | 0.027042 | 0.004612 | 0.004795 | 0.003845 | 0.006871 | 0.018376 | 0.003939 | 0.029452 | 0.015798 | 0.017393 | 0.004255 | 0.011771 |
Nuclear Periphery | 0.000524 | 0.000625 | 0.000885 | 0.001181 | 0.001022 | 0.001418 | 0.000798 | 0.001254 | 0.000802 | 0.001361 | 0.000830 | 0.001204 |
Nuclear Periphery Foci | 0.001499 | 0.004915 | 0.000836 | 0.000821 | 0.001519 | 0.002414 | 0.000364 | 0.001058 | 0.001510 | 0.002593 | 0.000538 | 0.000894 |
Nucleolus | 0.058600 | 0.082821 | 0.024966 | 0.038705 | 0.096659 | 0.077652 | 0.024855 | 0.050729 | 0.079815 | 0.078021 | 0.024896 | 0.042426 |
Nucleus | 0.592455* | 0.822101* | 0.888721* | 0.844863* | 0.817248* | 0.765689* | 0.903609* | 0.811197* | 0.717761* | 0.769718* | 0.898119* | 0.834442* |
Peroxisomes | 0.000646 | 0.002260 | 0.000975 | 0.000885 | 0.000675 | 0.001770 | 0.000727 | 0.006116 | 0.000662 | 0.001805 | 0.000818 | 0.002504 |
Vacuole | 0.007969 | 0.008081 | 0.005292 | 0.005195 | 0.001464 | 0.006501 | 0.006597 | 0.003402 | 0.004343 | 0.006614 | 0.006116 | 0.004640 |
Vacuole Periphery | 0.000262 | 0.000896 | 0.000250 | 0.000187 | 0.000248 | 0.001239 | 0.000147 | 0.000428 | 0.000254 | 0.001214 | 0.000185 | 0.000262 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -5.61 | -13.86 | -5.04 | 0.58 | -1.06 | -4.24 | -5.32 | -7.41 | -6.74 | -6.64 | -9.42 | -14.54 | -7.10 | -5.17 | -4.45 |
Bud Neck | 1.90 | -9.29 | -0.30 | -1.35 | 5.15 | 5.83 | 0.99 | -0.91 | -2.16 | -1.14 | 3.58 | -5.69 | -0.02 | -1.35 | 2.20 |
Bud Site | 0.32 | -5.19 | 0.74 | 0.32 | 4.81 | -2.06 | -0.15 | -2.24 | -1.75 | -2.22 | -1.33 | -3.78 | -1.65 | -0.85 | -0.31 |
Cell Periphery | 2.70 | 3.16 | 2.70 | -0.09 | 0.12 | -4.69 | -4.75 | -2.30 | -1.18 | -1.56 | -1.06 | 0.48 | -0.87 | -0.17 | -1.16 |
Cytoplasm | 22.97 | 32.58 | 12.11 | -0.76 | -1.09 | -8.03 | 1.94 | 4.34 | 9.86 | 2.75 | 10.18 | 26.48 | 8.30 | 3.23 | -0.59 |
Cytoplasmic Foci | 14.36 | 14.45 | 12.79 | -0.30 | -0.11 | -1.98 | 2.35 | 1.11 | 2.48 | -0.16 | 11.57 | 14.34 | 10.63 | 1.20 | -0.58 |
Eisosomes | -3.98 | -1.73 | -2.73 | 0.96 | -2.30 | -2.69 | -6.81 | -3.77 | -3.37 | -2.79 | -2.83 | -6.17 | -4.51 | -3.77 | -3.32 |
Endoplasmic Reticulum | -6.86 | -9.69 | -3.84 | -0.30 | -2.58 | -5.27 | -12.74 | -3.34 | -2.55 | -0.91 | -10.39 | -16.35 | -4.41 | -2.97 | -2.23 |
Endosome | 0.35 | 4.00 | 2.04 | 1.21 | -1.43 | -1.60 | 5.11 | 0.67 | 1.73 | -1.91 | 0.60 | 5.64 | 1.65 | 1.27 | -2.10 |
Golgi | -3.33 | -6.22 | 0.93 | 3.53 | 5.14 | -1.79 | 6.31 | -1.67 | -1.06 | -2.41 | -2.55 | -0.25 | -0.80 | 0.97 | -0.72 |
Lipid Particles | -1.67 | 1.21 | -1.16 | 0.07 | -1.66 | -1.52 | 2.44 | 0.09 | 1.56 | -2.25 | -1.22 | 2.62 | -1.38 | -0.43 | -2.21 |
Mitochondria | -1.07 | -8.18 | -4.11 | 0.92 | -0.18 | -1.24 | 1.64 | -2.60 | -0.13 | -3.87 | -2.00 | -2.09 | -3.79 | 0.96 | -3.04 |
Mitotic Spindle | -3.16 | -9.10 | -2.08 | -0.03 | 0.14 | -2.67 | 3.48 | -1.16 | -0.53 | -1.77 | -5.95 | -5.12 | -2.04 | -0.79 | -1.18 |
None | 11.61 | 12.91 | 13.50 | 1.77 | 2.70 | -5.26 | 8.20 | -0.67 | 0.99 | -1.48 | 0.70 | 14.83 | 5.79 | 4.22 | -1.13 |
Nuclear Periphery | -1.65 | -7.44 | -2.94 | -2.30 | -1.76 | -1.78 | -1.33 | -1.49 | 0.22 | -1.10 | -2.68 | -5.94 | -2.85 | -0.96 | -1.96 |
Nuclear Periphery Foci | -1.91 | 1.18 | 1.59 | 2.27 | 0.38 | -0.10 | 6.60 | 2.28 | 2.18 | -1.62 | -1.19 | 5.88 | 2.87 | 3.19 | -1.24 |
Nucleolus | -2.51 | -2.61 | 0.86 | 2.47 | 1.91 | 6.38 | 15.51 | 1.03 | -0.40 | -2.09 | 2.76 | 9.81 | 2.15 | 1.10 | -0.83 |
Nucleus | -3.40 | -9.80 | -4.76 | -1.38 | -1.10 | 1.73 | -11.97 | 0.31 | -0.07 | 2.56 | -4.80 | -16.43 | -2.70 | -1.09 | 1.69 |
Peroxisomes | -3.78 | -7.97 | -1.41 | 2.37 | 2.82 | -2.76 | -3.14 | -1.93 | -1.67 | -1.70 | -3.51 | -7.04 | -2.20 | -1.29 | -1.04 |
Vacuole | -0.41 | -1.00 | -1.16 | -1.04 | -0.96 | -5.34 | -7.67 | -2.22 | 0.76 | 3.50 | -3.85 | -6.99 | -1.57 | -0.83 | -0.02 |
Vacuole Periphery | -1.25 | -3.34 | -1.60 | 0.94 | 0.12 | -2.59 | 2.24 | -3.76 | 1.41 | -4.56 | -2.68 | -0.15 | -3.36 | 2.01 | -3.40 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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