Standard name
Human Ortholog
Description Dihydrolipoamide dehydrogenase; the lipoamide dehydrogenase component (E3) of the pyruvate dehydrogenase and 2-oxoglutarate dehydrogenase multi-enzyme complexes; PDH complex is concentrated in spots within the mitochondrial matrix, often near the ERMES complex and near peroxisomes; LPD1 has a paralog, IRC15, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – 0 0 – 0 – 0 0 0 0 0 0 – – – 0 0.05 0 0.14 0.06 0.07 0.07 0.31 0.27
Bud – 0 0 – 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Bud Neck – 0 0 – 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Bud Site – 0 0 – 0 – 0 0 0 0 0 0 – – – 0 0 0 – – – – – –
Cell Periphery – 0 0 – 0 – 0 0 0 0 0 0 – – – 0 0.07 0 0 0 0 0 0 0
Cytoplasm – 0 0.06 – 0 – 0 0 0 0 0 0.05 – – – 0 0.05 0 0 0 0 0 0 0
Endoplasmic Reticulum – 0 0 – 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Endosome – 0 0 – 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0 0 0.06 0
Golgi – 0 0 – 0.07 – 0 0 0 0 0 0 – – – 0.64 0.69 0.68 0.25 0.13 0.16 0.21 0.14 0.06
Mitochondria – 0.79 0.89 – 0.89 – 0.92 0.88 0.95 0.89 0.87 0.95 – – – 0.16 0.22 0 0.48 0.52 0.65 0.61 0.32 0.5
Nucleus – 0 0 – 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Nuclear Periphery – 0 0 – 0 – 0 0 0 0 0 0.05 – – – 0 0 0 0 0 0 0 0 0
Nucleolus – 0.18 0.11 – 0.16 – 0.31 0.34 0.11 0.2 0.27 0.21 – – – 0.05 0.07 0.15 0 0.08 0 0 0 0
Peroxisomes – 0 0 – 0 – 0 0 0 0 0 0 – – – 0 0 0.06 0 0 0.06 0 0.07 0.11
SpindlePole – 0 0 – 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane – 0.08 0 – 0 – 0.06 0.05 0 0.06 0 0 – – – 0.08 0.17 0.09 0 0.11 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – 2 0 – 1 – 0 2 0 0 1 0 – – – 3 5 1 4 7 4 1 11 5
Bud – 1 0 – 0 – 0 0 0 0 0 0 – – – 1 0 0 0 0 0 0 0 0
Bud Neck – 0 0 – 0 – 0 0 0 0 0 0 – – – 0 0 0 0 1 0 0 0 0
Bud Site – 0 0 – 0 – 0 0 0 0 0 0 – – – 0 0 0 – – – – – –
Cell Periphery – 0 0 – 0 – 0 0 0 0 0 0 – – – 1 7 1 0 0 0 0 0 0
Cytoplasm – 0 2 – 2 – 0 3 0 0 0 1 – – – 3 5 1 0 0 0 0 0 0
Endoplasmic Reticulum – 0 1 – 0 – 0 0 0 0 0 0 – – – 0 2 1 0 0 0 0 0 0
Endosome – 0 0 – 0 – 0 0 0 0 0 0 – – – 0 1 1 0 4 0 0 2 0
Golgi – 3 0 – 4 – 0 2 0 0 1 0 – – – 47 70 23 8 15 10 5 5 1
Mitochondria – 75 31 – 50 – 33 64 36 48 26 18 – – – 12 22 0 16 63 43 14 12 9
Nucleus – 0 0 – 0 – 1 0 0 0 0 0 – – – 1 0 0 0 0 0 0 0 0
Nuclear Periphery – 1 0 – 0 – 0 0 0 0 0 1 – – – 0 0 0 0 1 0 0 0 0
Nucleolus – 17 4 – 9 – 11 25 4 11 8 4 – – – 4 7 5 1 10 1 0 1 0
Peroxisomes – 4 0 – 1 – 0 0 0 0 0 0 – – – 2 2 2 0 2 3 0 2 2
SpindlePole – 1 0 – 0 – 0 0 0 0 0 0 – – – 0 2 0 0 0 0 0 0 0
Vac/Vac Membrane – 8 1 – 2 – 2 4 0 3 1 0 – – – 6 17 3 0 13 0 0 1 0
Unique Cell Count 95 35 56 36 73 38 54 30 19 74 101 34 35 123 67 24 39 19
Labelled Cell Count 112 39 69 47 100 40 62 37 24 80 140 38 35 123 67 24 39 19


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) – 21.9 52.5 – 48.9 – 47.8 41.6 41.1 31.3 35.2 43.2 – – – 39.3 40.8 39.9 – – –
Std Deviation (1e-4) – 9.0 17.5 – 17.5 – 21.5 16.3 16.3 11.3 11.0 19.8 – – – 17.2 15.2 17.9 – – –
Intensity Change (Log2) – – – – -0.1 – -0.13 -0.33 -0.35 -0.75 -0.58 -0.28 – – – -0.42 -0.36 -0.39 – – –


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches – 0 – 0 0 0 0 0 0 – – – 0 0 0
Bud – 0 – 0 0 0 0 0 0 – – – 0 0 0
Bud Neck – 0 – 0 0 0 0 0 0 – – – 0 0 0
Bud Site – 0 – 0 0 0 0 0 0 – – – 0 0 0
Cell Periphery – 0 – 0 0 0 0 0 0 – – – 0 0 0
Cytoplasm – 0 – 0 0 0 0 0 0 – – – 0 0 0
Endoplasmic Reticulum – 0 – 0 0 0 0 0 0 – – – 0 0 0
Endosome – 0 – 0 0 0 0 0 0 – – – 0 0 0
Golgi – 0 – 0 0 0 0 0 0 – – – 6.3 7.1 6.0
Mitochondria – 0.1 – 0.4 -0.1 1.0 0 -0.2 0.7 – – – -7.2 -7.0 -7.4
Nucleus – 0 – 0 0 0 0 0 0 – – – 0 0 0
Nuclear Periphery – 0 – 0 0 0 0 0 0 – – – 0 0 0
Nucleolus – 0.6 – 2.0 2.5 0 1.1 1.6 0 – – – 0 0 0
Peroxisomes – 0 – 0 0 0 0 0 0 – – – 0 0 0
SpindlePole – 0 – 0 0 0 0 0 0 – – – 0 0 0
Vacuole – 0 – 0 0 0 0 0 0 – – – 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 40.2447 57.0368 38.8773 29.0694 26.2181 42.2014 58.7567 80.1155 71.7098 82.4439 74.1525 72.029 27.7922 54.5068 54.5141 39.7448 39.9069 48.4336
Actin 0.0006 0.0008 0.0044 0.0005 0.1526 0.0004 0.0609 0.0097 0.0034 0.0001 0 0.0001 0.0166 0.0055 0.0229 0.0002 0.0645 0.0033
Bud 0.0001 0.0003 0.0001 0 0.0008 0 0.0003 0 0 0 0 0 0.0003 0.0001 0.0002 0 0.0003 0
Bud Neck 0 0 0.0073 0 0.0033 0 0.0011 0.0004 0 0 0 0 0.0002 0.0009 0.0019 0 0.0001 0
Bud Periphery 0.0001 0.0004 0.0002 0 0.0021 0 0.0005 0.0001 0 0 0 0 0.0003 0.0001 0.0002 0 0.0004 0
Bud Site 0.0001 0.0002 0.0009 0 0.0011 0 0.0013 0.0003 0.0001 0 0 0 0.0017 0.0002 0.0008 0 0.0008 0
Cell Periphery 0.0001 0.0004 0.0003 0 0.0008 0 0.0004 0.0004 0.0001 0 0 0.0002 0.0004 0.0004 0.0002 0 0.0007 0.0001
Cytoplasm 0.0001 0 0.0691 0 0.0002 0 0.0076 0 0.0005 0 0 0 0.0002 0 0 0 0 0
Cytoplasmic Foci 0.0367 0 0.0016 0.001 0.0041 0 0.0133 0.0002 0.0001 0 0 0 0.067 0.0011 0.0004 0 0.0032 0.0003
Eisosomes 0.0005 0.0005 0.0011 0.0003 0.0025 0.0002 0.0114 0.0111 0.0033 0.0004 0.0007 0.0006 0.001 0.0056 0.0012 0.0002 0.0008 0.0003
Endoplasmic Reticulum 0 0 0.0024 0 0.0021 0 0.0038 0 0.0002 0 0 0 0.0001 0 0 0 0 0
Endosome 0.0028 0 0.0037 0 0.0183 0 0.0064 0.0001 0.0013 0 0 0.0001 0.0043 0.0003 0.0002 0 0.0005 0.0005
Golgi 0.0017 0.0002 0.0081 0.0001 0.0916 0.0001 0.006 0.001 0.0015 0 0 0.0005 0.0108 0.0007 0.0009 0.0001 0.0044 0.0047
Lipid Particles 0.0234 0.0037 0.0033 0.0064 0.0212 0.0012 0.0377 0.0054 0.0008 0.0002 0.0003 0.0036 0.0996 0.0194 0.0096 0.0013 0.0608 0.0089
Mitochondria 0.9133 0.9819 0.8831 0.9652 0.6824 0.9962 0.7814 0.9576 0.9648 0.9989 0.9983 0.971 0.6285 0.9067 0.9467 0.9969 0.7824 0.9758
None 0 0.0001 0.0029 0 0.0002 0 0.0074 0 0.0001 0 0 0 0.001 0 0 0 0.0001 0
Nuclear Periphery 0 0 0.0008 0 0.0012 0 0.0087 0 0.0152 0 0 0.0002 0.0002 0 0 0 0.0003 0
Nucleolus 0.0003 0.0036 0.0002 0.0004 0.0005 0 0.009 0.0004 0.0001 0 0 0.0001 0.0079 0.0232 0.0017 0 0.0121 0.0005
Nucleus 0 0.0008 0.0016 0 0.0003 0 0.012 0.0001 0.0021 0 0 0 0.0005 0.001 0.0001 0 0.0009 0
Peroxisomes 0.0173 0.001 0.0047 0.0249 0.0061 0.0006 0.0079 0.0066 0.0025 0.0002 0.0002 0.0002 0.1521 0.0186 0.0057 0.0007 0.0418 0.0033
Punctate Nuclear 0.0001 0 0.0004 0 0.0002 0 0.0128 0 0 0 0 0 0.0016 0.0001 0 0 0.0001 0
Vacuole 0.0006 0.0006 0.001 0.0001 0.0017 0.0001 0.0012 0.0004 0.0005 0 0 0.0005 0.0016 0.0023 0.0005 0 0.0013 0.0002
Vacuole Periphery 0.0021 0.0056 0.0029 0.001 0.0068 0.0009 0.0089 0.0061 0.0036 0.0002 0.0003 0.0227 0.004 0.0138 0.0066 0.0005 0.0245 0.0018

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 154.4766 159.7929 154.682 157.6846 133.0469 149.2447 163.5611 171.4229 178.2599 160.305
Translational Efficiency 1.7211 1.5922 1.5468 1.8214 1.9565 1.9871 1.7782 1.9052 1.8026 1.8002

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
144 1377 1364 360 1719 221 1790 113 1863 1598 3154 473

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1566.18 2068.25 2194.11 3983.25 1919.94 2198.50 3063.26 5002.33 1892.60 2086.26 2687.38 4226.71
Standard Deviation 689.17 657.62 716.33 1191.42 606.42 803.88 891.59 1639.42 620.45 681.21 926.54 1382.50
Intensity Change Log 2 — 0.401161 0.486386 1.346696 — 0.195458 0.674007 1.381539 — 0.291515 0.592719 1.365989

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.024777 0.010007 0.008282 0.026002 0.003078 0.003947 0.006828 0.038391 0.004755 0.009169 0.007457 0.028962
Bud Neck 0.012168 0.002178 0.002148 0.002360 0.000716 0.010614 0.001233 0.013748 0.001602 0.003345 0.001629 0.005080
Bud Site 0.012166 0.002323 0.001109 0.003412 0.000623 0.014481 0.000641 0.012594 0.001515 0.004005 0.000843 0.005606
Cell Periphery 0.000288 0.000209 0.000068 0.000554 0.000041 0.001612 0.000072 0.000650 0.000060 0.000403 0.000070 0.000577
Cytoplasm 0.001137 0.000370 0.000297 0.000414 0.000178 0.001045 0.000103 0.000329 0.000253 0.000463 0.000187 0.000393
Cytoplasmic Foci 0.070951 0.020349 0.027242 0.010975 0.015341 0.052619 0.013334 0.033878 0.019640 0.024812 0.019349 0.016446
Eisosomes 0.004657 0.004330 0.001568 0.005646 0.002706 0.001127 0.002038 0.009750 0.002857 0.003887 0.001834 0.006626
Endoplasmic Reticulum 0.000586 0.000154 0.000120 0.001738 0.000084 0.000551 0.000092 0.002413 0.000123 0.000209 0.000104 0.001899
Endosome 0.014236 0.001418 0.001418 0.001239 0.000474 0.009047 0.000600 0.003629 0.001538 0.002473 0.000954 0.001810
Golgi 0.071482 0.018055 0.034839 0.079187 0.012409 0.029463 0.026207 0.154310* 0.016975 0.019633 0.029940 0.097134
Lipid Particles 0.028089 0.009100 0.018335 0.007392 0.005948 0.013910 0.009161 0.018416 0.007659 0.009765 0.013128 0.010026
Mitochondria 0.656236* 0.895513* 0.861182* 0.813465* 0.934767* 0.748238* 0.902119* 0.586015* 0.913238* 0.875146* 0.884415* 0.759127*
Mitotic Spindle 0.003203 0.000282 0.003888 0.000877 0.000727 0.006036 0.000932 0.007623 0.000918 0.001078 0.002210 0.002488
None 0.002309 0.003357 0.005081 0.007734 0.003994 0.003669 0.006669 0.012738 0.003863 0.003400 0.005983 0.008930
Nuclear Periphery 0.000466 0.000169 0.002165 0.001362 0.000384 0.002066 0.001285 0.004551 0.000390 0.000431 0.001666 0.002124
Nuclear Periphery Foci 0.001447 0.000195 0.000525 0.000417 0.000409 0.001449 0.000274 0.001921 0.000489 0.000369 0.000382 0.000776
Nucleolus 0.001989 0.001303 0.000781 0.000530 0.000776 0.008085 0.000526 0.004940 0.000869 0.002241 0.000636 0.001583
Nucleus 0.000499 0.000293 0.000059 0.000235 0.000081 0.000624 0.000038 0.000410 0.000113 0.000339 0.000047 0.000277
Peroxisomes 0.073618 0.021411 0.010562 0.003866 0.012260 0.022585 0.003239 0.007570 0.017002 0.021574 0.006406 0.004751
Vacuole 0.013265 0.005287 0.005232 0.007645 0.001910 0.041080 0.004781 0.020559 0.002788 0.010237 0.004976 0.010730
Vacuole Periphery 0.006430 0.003696 0.015099 0.024951 0.003096 0.027751 0.019827 0.065565 0.003354 0.007023 0.017782 0.034654

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 2.37 2.68 -0.17 -3.29 -3.72 -0.56 -3.17 -3.45 -3.33 -3.07 -3.25 -2.76 -5.54 -4.42 -4.91
Bud Neck 3.17 3.19 3.06 -0.35 -0.42 -3.73 -2.43 -3.54 -1.02 -3.41 -3.08 -0.12 -3.42 -1.82 -3.46
Bud Site 1.84 2.07 1.49 -0.76 -1.44 -2.71 -0.08 -1.44 0.14 -1.43 -2.53 1.38 -1.77 -0.76 -2.06
Cell Periphery 0.80 3.10 -1.54 -1.98 -2.95 -1.58 -3.90 -6.86 0.96 -6.51 -2.36 -1.26 -4.03 -0.94 -3.96
Cytoplasm 3.66 3.95 2.28 -0.58 -0.91 -2.06 1.99 -1.47 1.65 -2.29 -2.74 1.44 -1.28 0.07 -1.71
Cytoplasmic Foci 5.01 4.31 5.81 3.08 5.38 -5.09 1.26 -2.24 1.70 -2.49 -2.24 0.14 0.83 2.42 0.80
Eisosomes 0.33 3.33 -0.83 -1.55 -5.38 5.05 2.42 -3.44 -4.22 -3.78 -2.54 4.11 -4.79 -3.32 -6.32
Endoplasmic Reticulum 3.86 4.27 -1.37 -1.89 -1.93 -1.69 -0.45 -4.77 -3.31 -4.75 -1.86 0.97 -2.74 -2.60 -2.77
Endosome 3.95 3.95 4.00 0.54 0.62 -4.06 -0.95 -3.42 2.37 -3.29 -2.06 1.97 -0.69 1.45 -2.87
Golgi 4.29 2.92 -0.49 -6.56 -4.69 -3.01 -5.92 -7.19 -6.10 -6.47 -1.14 -5.96 -9.26 -8.92 -7.76
Lipid Particles 2.82 1.43 2.96 0.68 3.96 -2.35 -2.63 -2.73 -0.80 -2.03 -1.41 -4.17 -1.06 -0.11 1.44
Mitochondria -8.55 -7.29 -5.00 5.31 3.09 8.53 5.66 11.05 4.31 10.01 5.17 5.02 10.48 7.67 8.68
Mitotic Spindle 4.10 -0.62 2.65 -1.98 2.92 -1.82 -0.58 -2.45 -0.39 -2.38 -0.33 -2.70 -2.18 -1.83 -0.48
None -3.56 -7.70 -7.69 -6.35 -3.69 0.71 -7.24 -7.45 -7.41 -5.06 1.82 -7.41 -8.21 -9.10 -4.73
Nuclear Periphery 4.13 -6.58 -2.53 -3.43 1.85 -2.01 -3.43 -3.59 -1.74 -2.77 -0.26 -6.28 -4.32 -4.19 -1.09
Nuclear Periphery Foci 1.80 1.32 1.46 -2.27 0.85 -3.00 0.69 -2.00 -0.60 -2.25 0.63 0.56 -1.09 -2.09 -2.05
Nucleolus 1.40 2.66 3.07 2.99 1.26 -2.28 1.39 -2.20 0.78 -2.33 -2.72 1.36 -1.54 0.85 -2.08
Nucleus 1.10 3.68 0.69 -0.15 -1.37 -3.85 1.69 -2.17 0.50 -2.41 -1.96 2.60 -1.63 -0.16 -2.05
Peroxisomes 4.35 5.30 5.88 6.91 3.36 -2.03 5.36 1.08 2.39 -1.07 -1.71 5.54 5.49 6.94 1.07
Vacuole 2.29 2.36 1.32 -0.88 -0.93 -6.49 -5.18 -4.02 2.46 -3.43 -5.98 -4.36 -3.55 -0.33 -2.62
Vacuole Periphery 1.78 -4.46 -4.78 -5.87 -2.58 -5.54 -11.29 -5.53 -3.11 -4.01 -4.38 -13.76 -7.96 -6.93 -4.17
Standard name
Human Ortholog
Description Dihydrolipoamide dehydrogenase; the lipoamide dehydrogenase component (E3) of the pyruvate dehydrogenase and 2-oxoglutarate dehydrogenase multi-enzyme complexes; PDH complex is concentrated in spots within the mitochondrial matrix, often near the ERMES complex and near peroxisomes; LPD1 has a paralog, IRC15, that arose from the whole genome duplication
Localization
Cell Percentages mitochondrion (96%)
Cell Cycle Regulation No
Subcompartmental Group mito-3

Lpd1

Lpd1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Lpd1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available