Standard name | |
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Human Ortholog | |
Description | Dihydrolipoamide dehydrogenase; the lipoamide dehydrogenase component (E3) of the pyruvate dehydrogenase and 2-oxoglutarate dehydrogenase multi-enzyme complexes; PDH complex is concentrated in spots within the mitochondrial matrix, often near the ERMES complex and near peroxisomes; LPD1 has a paralog, IRC15, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | – | 2 | 0 | – | 1 | – | 0 | 2 | 0 | 0 | 1 | 0 | – | – | – | 3 | 5 | 1 | 4 | 7 | 4 | 1 | 11 | 5 |
Bud | – | 1 | 0 | – | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | – | 0 | 0 | – | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
Bud Site | – | 0 | 0 | – | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | – | 0 | 0 | – | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 1 | 7 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | – | 0 | 2 | – | 2 | – | 0 | 3 | 0 | 0 | 0 | 1 | – | – | – | 3 | 5 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | – | 0 | 1 | – | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | – | 0 | 0 | – | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 1 | 1 | 0 | 4 | 0 | 0 | 2 | 0 |
Golgi | – | 3 | 0 | – | 4 | – | 0 | 2 | 0 | 0 | 1 | 0 | – | – | – | 47 | 70 | 23 | 8 | 15 | 10 | 5 | 5 | 1 |
Mitochondria | – | 75 | 31 | – | 50 | – | 33 | 64 | 36 | 48 | 26 | 18 | – | – | – | 12 | 22 | 0 | 16 | 63 | 43 | 14 | 12 | 9 |
Nucleus | – | 0 | 0 | – | 0 | – | 1 | 0 | 0 | 0 | 0 | 0 | – | – | – | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | – | 1 | 0 | – | 0 | – | 0 | 0 | 0 | 0 | 0 | 1 | – | – | – | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
Nucleolus | – | 17 | 4 | – | 9 | – | 11 | 25 | 4 | 11 | 8 | 4 | – | – | – | 4 | 7 | 5 | 1 | 10 | 1 | 0 | 1 | 0 |
Peroxisomes | – | 4 | 0 | – | 1 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 2 | 2 | 2 | 0 | 2 | 3 | 0 | 2 | 2 |
SpindlePole | – | 1 | 0 | – | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | – | 8 | 1 | – | 2 | – | 2 | 4 | 0 | 3 | 1 | 0 | – | – | – | 6 | 17 | 3 | 0 | 13 | 0 | 0 | 1 | 0 |
Unique Cell Count | 95 | 35 | 56 | 36 | 73 | 38 | 54 | 30 | 19 | 74 | 101 | 34 | 35 | 123 | 67 | 24 | 39 | 19 | ||||||
Labelled Cell Count | 112 | 39 | 69 | 47 | 100 | 40 | 62 | 37 | 24 | 80 | 140 | 38 | 35 | 123 | 67 | 24 | 39 | 19 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | – | 21.9 | 52.5 | – | 48.9 | – | 47.8 | 41.6 | 41.1 | 31.3 | 35.2 | 43.2 | – | – | – | 39.3 | 40.8 | 39.9 | – | – | – |
Std Deviation (1e-4) | – | 9.0 | 17.5 | – | 17.5 | – | 21.5 | 16.3 | 16.3 | 11.3 | 11.0 | 19.8 | – | – | – | 17.2 | 15.2 | 17.9 | – | – | – |
Intensity Change (Log2) | – | – | – | – | -0.1 | – | -0.13 | -0.33 | -0.35 | -0.75 | -0.58 | -0.28 | – | – | – | -0.42 | -0.36 | -0.39 | – | – | – |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | – | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 |
Bud | – | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 |
Bud Neck | – | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 |
Bud Site | – | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 |
Cell Periphery | – | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 |
Cytoplasm | – | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 |
Endoplasmic Reticulum | – | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 |
Endosome | – | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 |
Golgi | – | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 6.3 | 7.1 | 6.0 |
Mitochondria | – | 0.1 | – | 0.4 | -0.1 | 1.0 | 0 | -0.2 | 0.7 | – | – | – | -7.2 | -7.0 | -7.4 |
Nucleus | – | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 |
Nuclear Periphery | – | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 |
Nucleolus | – | 0.6 | – | 2.0 | 2.5 | 0 | 1.1 | 1.6 | 0 | – | – | – | 0 | 0 | 0 |
Peroxisomes | – | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 |
SpindlePole | – | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 |
Vacuole | – | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 40.2447 | 57.0368 | 38.8773 | 29.0694 | 26.2181 | 42.2014 | 58.7567 | 80.1155 | 71.7098 | 82.4439 | 74.1525 | 72.029 | 27.7922 | 54.5068 | 54.5141 | 39.7448 | 39.9069 | 48.4336 |
Actin | 0.0006 | 0.0008 | 0.0044 | 0.0005 | 0.1526 | 0.0004 | 0.0609 | 0.0097 | 0.0034 | 0.0001 | 0 | 0.0001 | 0.0166 | 0.0055 | 0.0229 | 0.0002 | 0.0645 | 0.0033 |
Bud | 0.0001 | 0.0003 | 0.0001 | 0 | 0.0008 | 0 | 0.0003 | 0 | 0 | 0 | 0 | 0 | 0.0003 | 0.0001 | 0.0002 | 0 | 0.0003 | 0 |
Bud Neck | 0 | 0 | 0.0073 | 0 | 0.0033 | 0 | 0.0011 | 0.0004 | 0 | 0 | 0 | 0 | 0.0002 | 0.0009 | 0.0019 | 0 | 0.0001 | 0 |
Bud Periphery | 0.0001 | 0.0004 | 0.0002 | 0 | 0.0021 | 0 | 0.0005 | 0.0001 | 0 | 0 | 0 | 0 | 0.0003 | 0.0001 | 0.0002 | 0 | 0.0004 | 0 |
Bud Site | 0.0001 | 0.0002 | 0.0009 | 0 | 0.0011 | 0 | 0.0013 | 0.0003 | 0.0001 | 0 | 0 | 0 | 0.0017 | 0.0002 | 0.0008 | 0 | 0.0008 | 0 |
Cell Periphery | 0.0001 | 0.0004 | 0.0003 | 0 | 0.0008 | 0 | 0.0004 | 0.0004 | 0.0001 | 0 | 0 | 0.0002 | 0.0004 | 0.0004 | 0.0002 | 0 | 0.0007 | 0.0001 |
Cytoplasm | 0.0001 | 0 | 0.0691 | 0 | 0.0002 | 0 | 0.0076 | 0 | 0.0005 | 0 | 0 | 0 | 0.0002 | 0 | 0 | 0 | 0 | 0 |
Cytoplasmic Foci | 0.0367 | 0 | 0.0016 | 0.001 | 0.0041 | 0 | 0.0133 | 0.0002 | 0.0001 | 0 | 0 | 0 | 0.067 | 0.0011 | 0.0004 | 0 | 0.0032 | 0.0003 |
Eisosomes | 0.0005 | 0.0005 | 0.0011 | 0.0003 | 0.0025 | 0.0002 | 0.0114 | 0.0111 | 0.0033 | 0.0004 | 0.0007 | 0.0006 | 0.001 | 0.0056 | 0.0012 | 0.0002 | 0.0008 | 0.0003 |
Endoplasmic Reticulum | 0 | 0 | 0.0024 | 0 | 0.0021 | 0 | 0.0038 | 0 | 0.0002 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0.0028 | 0 | 0.0037 | 0 | 0.0183 | 0 | 0.0064 | 0.0001 | 0.0013 | 0 | 0 | 0.0001 | 0.0043 | 0.0003 | 0.0002 | 0 | 0.0005 | 0.0005 |
Golgi | 0.0017 | 0.0002 | 0.0081 | 0.0001 | 0.0916 | 0.0001 | 0.006 | 0.001 | 0.0015 | 0 | 0 | 0.0005 | 0.0108 | 0.0007 | 0.0009 | 0.0001 | 0.0044 | 0.0047 |
Lipid Particles | 0.0234 | 0.0037 | 0.0033 | 0.0064 | 0.0212 | 0.0012 | 0.0377 | 0.0054 | 0.0008 | 0.0002 | 0.0003 | 0.0036 | 0.0996 | 0.0194 | 0.0096 | 0.0013 | 0.0608 | 0.0089 |
Mitochondria | 0.9133 | 0.9819 | 0.8831 | 0.9652 | 0.6824 | 0.9962 | 0.7814 | 0.9576 | 0.9648 | 0.9989 | 0.9983 | 0.971 | 0.6285 | 0.9067 | 0.9467 | 0.9969 | 0.7824 | 0.9758 |
None | 0 | 0.0001 | 0.0029 | 0 | 0.0002 | 0 | 0.0074 | 0 | 0.0001 | 0 | 0 | 0 | 0.001 | 0 | 0 | 0 | 0.0001 | 0 |
Nuclear Periphery | 0 | 0 | 0.0008 | 0 | 0.0012 | 0 | 0.0087 | 0 | 0.0152 | 0 | 0 | 0.0002 | 0.0002 | 0 | 0 | 0 | 0.0003 | 0 |
Nucleolus | 0.0003 | 0.0036 | 0.0002 | 0.0004 | 0.0005 | 0 | 0.009 | 0.0004 | 0.0001 | 0 | 0 | 0.0001 | 0.0079 | 0.0232 | 0.0017 | 0 | 0.0121 | 0.0005 |
Nucleus | 0 | 0.0008 | 0.0016 | 0 | 0.0003 | 0 | 0.012 | 0.0001 | 0.0021 | 0 | 0 | 0 | 0.0005 | 0.001 | 0.0001 | 0 | 0.0009 | 0 |
Peroxisomes | 0.0173 | 0.001 | 0.0047 | 0.0249 | 0.0061 | 0.0006 | 0.0079 | 0.0066 | 0.0025 | 0.0002 | 0.0002 | 0.0002 | 0.1521 | 0.0186 | 0.0057 | 0.0007 | 0.0418 | 0.0033 |
Punctate Nuclear | 0.0001 | 0 | 0.0004 | 0 | 0.0002 | 0 | 0.0128 | 0 | 0 | 0 | 0 | 0 | 0.0016 | 0.0001 | 0 | 0 | 0.0001 | 0 |
Vacuole | 0.0006 | 0.0006 | 0.001 | 0.0001 | 0.0017 | 0.0001 | 0.0012 | 0.0004 | 0.0005 | 0 | 0 | 0.0005 | 0.0016 | 0.0023 | 0.0005 | 0 | 0.0013 | 0.0002 |
Vacuole Periphery | 0.0021 | 0.0056 | 0.0029 | 0.001 | 0.0068 | 0.0009 | 0.0089 | 0.0061 | 0.0036 | 0.0002 | 0.0003 | 0.0227 | 0.004 | 0.0138 | 0.0066 | 0.0005 | 0.0245 | 0.0018 |
R1 | R2 | |||||||||
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G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 154.4766 | 159.7929 | 154.682 | 157.6846 | 133.0469 | 149.2447 | 163.5611 | 171.4229 | 178.2599 | 160.305 |
Translational Efficiency | 1.7211 | 1.5922 | 1.5468 | 1.8214 | 1.9565 | 1.9871 | 1.7782 | 1.9052 | 1.8026 | 1.8002 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
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37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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