Standard name
Human Ortholog
Description Transcriptional activator of nitrogen catabolite repression genes; contains a GATA-1-type zinc finger DNA-binding motif; activity and localization regulated by nitrogen limitation and Ure2p; different translational starts produce two major and two minor isoforms that are differentially regulated and localized
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.8 0.92 0.55 0.1 0.07 0.06 0.08 0.06 0.08 0.08 0.08 0.07 0.98 0.99 0.99 0.7 0.91 0.93 0.45 0.73 0.31 0.63 0.79 0.68
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0.12 0 0.07 0.05 0.14 0.27 0.34 0.26 0.27 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0.05 0.74 0.7 0.53 0.56 0.62 0.49 0.52 0.47 0.55 0 0 0 0 0 0 0.32 0.15 0.37 0.19 0.07 0.05
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0.19 0.09 0.15 0.13 0.12 0.13 0.18 0.18 0.16 0 0 0 0 0 0 0 0 0.05 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.3 0.1 0.47 0 0.16 0.29 0.23 0.15 0.19 0.14 0.15 0.17 0 0 0 0.35 0.1 0 0.06 0.05 0.14 0.07 0 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2
Bud 0 0 0 0 0 0 2 1 3 3 0 1 0 0 0 1 0 0 5 6 2 0 1 4
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 1 0 0 1
Bud Site 0 0 0 0 0 0 1 1 4 4 1 6 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 3 3 0 2 2 2 0 0 0 0 0 0
Cytoplasm 80 368 74 4 8 11 22 17 17 20 18 19 124 319 263 154 129 79 55 302 56 63 178 142
Endoplasmic Reticulum 0 4 0 0 0 0 1 0 0 0 0 0 1 1 0 5 3 4 0 0 3 0 3 3
Endosome 0 0 0 0 1 1 6 2 0 0 1 0 0 0 0 3 1 0 1 2 5 1 3 10
Golgi 0 4 4 0 0 0 0 0 0 0 0 0 0 0 0 9 5 3 0 0 0 0 0 0
Mitochondria 0 1 1 5 1 12 15 42 55 90 61 72 0 0 1 2 1 2 0 0 2 0 0 0
Nucleus 2 5 7 31 80 93 160 179 99 139 112 149 0 0 2 5 0 0 40 61 67 19 15 10
Nuclear Periphery 0 0 0 0 0 0 0 0 0 2 1 0 0 0 0 0 0 0 2 2 3 2 5 3
Nucleolus 1 1 2 8 10 26 38 34 26 47 42 44 0 1 0 0 2 0 5 4 8 2 0 3
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 2 4 1 0 6 8
Vac/Vac Membrane 30 42 63 0 18 51 66 44 38 36 36 45 0 3 0 78 14 2 7 22 25 6 5 10
Unique Cell Count 100 401 134 42 114 176 284 291 201 266 239 271 126 322 265 220 142 85 124 415 184 102 225 210
Labelled Cell Count 113 425 151 48 118 194 311 321 242 341 272 336 128 327 266 260 158 92 124 415 184 102 225 210


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 19.5 21.7 20.2 16.5 15.8 12.4 11.0 10.8 10.0 9.5 9.6 9.7 18.0 17.0 15.7 22.8 29.7 30.7 17.4 18.3 20.1
Std Deviation (1e-4) 2.8 4.0 4.1 5.6 4.2 2.3 2.4 2.3 2.1 2.2 2.1 2.5 3.3 2.7 2.4 6.7 8.2 9.2 3.8 3.0 4.1
Intensity Change (Log2) – – – -0.29 -0.36 -0.71 -0.88 -0.91 -1.02 -1.09 -1.07 -1.05 -0.17 -0.25 -0.36 0.17 0.55 0.6 -0.22 -0.14 -0.01


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -5.2 -8.0 -9.6 -10.8 -11.5 -9.4 -10.6 -10.3 -10.9 8.2 12.4 11.5 2.8 6.7 5.9
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 2.6 2.3 4.3 6.4 7.5 6.2 6.4 0 0 0 0 0 0
Nucleus 9.4 10.7 8.9 10.0 10.9 8.5 9.2 8.3 9.7 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 2.7 4.0 3.9 3.5 3.7 4.7 4.6 4.4 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -5.5 -5.2 -3.3 -4.9 -7.0 -5.5 -7.3 -6.7 -6.5 -8.8 -12.7 -12.2 -2.2 -6.9 -7.1

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.8892 4.7486 4.3075 4.2221 3.3899 4.389 5.0964 5.7427 4.808 4.2875 3.9535 5.2951 7.2372 7.1987 6.557 6.5659 5.7943 6.8135
Actin 0.013 0.0006 0.0017 0.0412 0.0019 0.0004 0.08 0.0006 0.0076 0.0119 0.0031 0.0069 0.0502 0.0044 0.0213 0.0014 0.0263 0.0015
Bud 0.0004 0.0003 0.0002 0.0003 0.0002 0.0003 0.0004 0.0002 0.0003 0.0002 0.0001 0.0003 0.0019 0.0006 0.0008 0.0004 0.0009 0.0003
Bud Neck 0.0003 0.0003 0.0005 0.0012 0.0007 0.0082 0.0031 0.0002 0.0004 0.0006 0.0004 0.0016 0.0018 0.0007 0.0059 0.0014 0.001 0.0025
Bud Periphery 0.0006 0.0001 0.0001 0.0002 0.0001 0.0001 0.0005 0.0001 0.0002 0.0002 0.0001 0.0004 0.0019 0.0003 0.0011 0.0001 0.0015 0.0002
Bud Site 0.0008 0.0013 0.0004 0.0007 0.0003 0.0004 0.002 0.0006 0.0008 0.0013 0.0003 0.0005 0.0037 0.003 0.0029 0.0005 0.0012 0.0002
Cell Periphery 0.0001 0.0005 0.0001 0.0002 0.0001 0.0001 0.0002 0.0001 0.0001 0.0001 0.0001 0.0001 0.0002 0.0002 0.0005 0 0.0001 0
Cytoplasm 0.8082 0.9261 0.8671 0.9116 0.8845 0.7155 0.6286 0.8715 0.8047 0.8372 0.8308 0.8278 0.6471 0.7786 0.7372 0.645 0.7115 0.6694
Cytoplasmic Foci 0.0072 0.0018 0.0017 0.0024 0.0121 0.0078 0.0304 0.0026 0.0102 0.0055 0.023 0.0086 0.0347 0.0058 0.0139 0.0105 0.0194 0.0064
Eisosomes 0.0001 0 0 0.003 0 0 0.0007 0 0.0001 0.0003 0 0 0.0003 0 0.0001 0 0.0002 0
Endoplasmic Reticulum 0.01 0.0051 0.0038 0.0046 0.01 0.0058 0.0088 0.0071 0.0078 0.003 0.0087 0.0039 0.0081 0.0035 0.0046 0.0037 0.005 0.0039
Endosome 0.0235 0.001 0.0027 0.0018 0.0109 0.0033 0.0586 0.0014 0.011 0.0049 0.05 0.0074 0.059 0.0028 0.0172 0.005 0.0284 0.006
Golgi 0.0086 0.0001 0.0031 0.0007 0.0007 0.0005 0.0211 0.0001 0.0035 0.0053 0.0069 0.0043 0.0196 0.0009 0.0058 0.0003 0.034 0.0023
Lipid Particles 0.0188 0.0001 0.001 0.0003 0.0009 0.0003 0.0263 0.0001 0.0009 0.0094 0.0318 0.0038 0.0171 0.0002 0.0025 0.0001 0.014 0.0011
Mitochondria 0.0139 0.0003 0.0121 0.0005 0.0003 0.0124 0.0105 0.0001 0.001 0.0164 0.0015 0.005 0.0113 0.0014 0.0091 0.0005 0.0289 0.0016
None 0.0078 0.0162 0.0361 0.004 0.019 0.0153 0.0666 0.0981 0.1063 0.0815 0.0281 0.0324 0.0277 0.0667 0.0436 0.1121 0.0032 0.0212
Nuclear Periphery 0.0134 0.003 0.0094 0.0026 0.0075 0.0091 0.0079 0.0033 0.0028 0.0013 0.0033 0.0061 0.019 0.0048 0.0055 0.0073 0.0073 0.008
Nucleolus 0.0003 0.0001 0.0002 0 0.0003 0.0003 0.0021 0 0.0001 0.0001 0.0001 0.0002 0.0007 0.0003 0.0002 0.0003 0.0001 0.0004
Nucleus 0.0636 0.0407 0.0558 0.0217 0.0408 0.2128 0.0298 0.0122 0.0358 0.0141 0.003 0.0813 0.057 0.1211 0.1158 0.2002 0.0705 0.2641
Peroxisomes 0.0026 0 0.0003 0.0007 0.0004 0.0005 0.0114 0 0.004 0.0054 0.0047 0.0023 0.0208 0.001 0.0048 0.0006 0.0389 0.0006
Punctate Nuclear 0.0034 0.0005 0.0008 0.0004 0.0068 0.0022 0.0061 0.001 0.0012 0.0006 0.0025 0.0061 0.0135 0.0026 0.0041 0.009 0.0035 0.009
Vacuole 0.0024 0.0018 0.0016 0.0016 0.0022 0.0041 0.0034 0.0005 0.0011 0.0005 0.0013 0.0008 0.0023 0.0007 0.0024 0.0011 0.0021 0.0011
Vacuole Periphery 0.001 0.0001 0.0011 0.0003 0.0004 0.0005 0.0018 0.0001 0.0003 0.0002 0.0004 0.0003 0.0021 0.0001 0.0007 0.0003 0.0019 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 5.0384 10.2949 7.6392 16.2955 12.1317 10.0712 10.0838 8.7818 13.6375 19.095
Translational Efficiency 0.9494 0.3897 0.6228 0.9057 1.2022 0.4722 0.4065 0.7754 0.9943 0.9261

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✔
Standard name
Human Ortholog
Description Transcriptional activator of nitrogen catabolite repression genes; contains a GATA-1-type zinc finger DNA-binding motif; activity and localization regulated by nitrogen limitation and Ure2p; different translational starts produce two major and two minor isoforms that are differentially regulated and localized
Localization
Cell Percentages cytoplasm (38%), nucleus (1%), mixed (61%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Gat1

Gat1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Gat1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available