Standard name
Human Ortholog
Description Unconventional prefoldin protein involved in translation initiation; required for correct assembly of RNAP I, II, and III in an Rpb5p-dependent manner; shuttles between nucleus and cytoplasm; mutants have inappropriate expression of nutrient sensitive genes due to translational derepression of Gcn4p transcription factor; diploid mutants show random budding; ortholog of human URI/RMP
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.06 0.05 0 0 0 0 0 0
Cytoplasm 0.87 0.91 0.92 0.94 0.94 0.91 0.91 0.91 0.85 – 0.78 0.86 0.96 0.97 0.99 0.92 0.85 0.83 0.72 0.78 0.77 0.58 0.66 0.54
Endoplasmic Reticulum 0.07 0.05 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.05 0 0 0.17 0.13 0.14
Endosome 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.08 0 0 0.09 0.06 0.15 0.15 0.11 0.3 – 0.39 0.17 0 0 0 0 0 0 0 0 0.1 0.08 0.07 0.1
Nucleus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0.05 0.07 0.11 0 0.07 0 0.09 0.05 0.08
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 3 6 0 1 0 0 0 0 – 0 0 1 0 0 3 5 3 2 1 3 2 3 4
Bud 0 4 8 2 0 4 4 4 9 – 16 5 0 3 0 0 0 0 0 0 0 1 0 2
Bud Neck 0 0 0 0 1 1 1 2 2 – 2 5 0 0 0 0 0 0 0 3 3 0 1 2
Bud Site 0 0 0 0 0 0 2 0 0 – 0 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 2 13 3 4 5 2 9 3 – 3 8 3 9 0 21 16 12 0 0 0 0 0 1
Cytoplasm 103 202 321 187 355 379 504 527 418 – 354 396 133 271 285 474 236 217 97 201 291 63 153 134
Endoplasmic Reticulum 8 11 3 0 0 1 1 0 0 – 0 5 2 3 2 14 12 9 6 8 8 18 31 35
Endosome 1 5 8 2 2 1 4 4 1 – 1 1 1 3 0 4 7 6 9 11 4 0 5 8
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 2 6 10 2 2 2 0 2 4
Mitochondria 10 1 2 18 22 61 84 66 147 – 178 77 2 0 1 6 3 2 5 6 38 8 17 24
Nucleus 2 0 0 0 5 4 6 6 6 – 7 3 0 1 1 8 4 2 0 0 2 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 1 0 – 1 0 1 0 0 1 0 0 3 1 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 1 0 – 0 0 0 0 1 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 3 0 2 1 2 6 2 – 2 5 0 0 0 0 1 0 1 0 2 0 0 1
Vac/Vac Membrane 0 1 13 5 10 8 4 11 7 – 4 3 1 1 0 26 19 28 4 18 17 10 12 18
Unique Cell Count 118 222 348 200 378 418 551 580 492 455 461 139 280 289 518 279 261 135 257 380 110 232 247
Labelled Cell Count 124 229 377 217 402 465 614 637 595 568 509 144 291 290 559 309 289 135 257 380 110 232 247


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.7 7.8 7.1 6.5 6.5 5.1 5.1 5.4 4.2 – 3.8 4.2 7.3 7.4 7.0 12.8 13.3 13.1 6.9 6.8 7.4
Std Deviation (1e-4) 1.1 1.0 0.8 1.1 1.1 0.9 1.3 1.4 1.2 – 0.9 0.7 1.2 1.1 1.1 2.1 2.8 1.9 1.2 1.3 1.5
Intensity Change (Log2) – – – -0.12 -0.12 -0.47 -0.46 -0.38 -0.74 – -0.9 -0.75 0.04 0.06 -0.01 0.86 0.92 0.89 -0.03 -0.06 0.07


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud 0 0 -1.5 0 0 0 – 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cell Periphery -1.5 -2.4 -2.3 -3.8 -2.1 0 – 0 0 0 -0.4 -3.3 0.2 1.2 0.5
Cytoplasm 0.5 0.9 -0.8 -0.4 -0.7 -3.2 – -5.5 -2.8 1.4 2.4 3.7 -0.4 -3.0 -3.5
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 1.9 2.8 2.3
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0.2 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 5.1 3.9 7.0 7.3 6.1 0 – 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Vacuole -0.8 -0.8 -1.5 -3.2 -1.7 -2.2 – -2.8 -3.1 0 -2.9 -3.3 0.9 1.7 3.4

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.4297 4.8909 4.133 4.0993 3.1415 4.0 2.3654 3.0525 2.8976 2.5414 2.4976 2.8347 1.7297 2.6773 2.3473 2.1865 2.377 2.2622
Actin 0.0713 0.0005 0.0274 0.0154 0.0004 0.0096 0.0232 0.0028 0.0044 0.0007 0.002 0.0066 0.0086 0.001 0.0068 0.0129 0.0002 0.0029
Bud 0.0007 0.0003 0.001 0.001 0.0005 0.0002 0.0012 0.0025 0.0018 0.0017 0.0005 0.0011 0.0004 0.0004 0.0004 0.0005 0.001 0.0002
Bud Neck 0.0028 0.0002 0.0007 0.001 0.0004 0.0021 0.0072 0.0003 0.0011 0.0007 0.0008 0.0018 0.0017 0.0002 0.0003 0.0007 0.0005 0.0041
Bud Periphery 0.0009 0.0001 0.001 0.0009 0.0003 0.0002 0.0013 0.0025 0.0019 0.0012 0.0004 0.0034 0.0003 0.0001 0.0002 0.0002 0.0011 0.0001
Bud Site 0.014 0.0021 0.0051 0.0009 0.0002 0.0002 0.0062 0.0115 0.0115 0.0006 0.0009 0.0005 0.0011 0.0021 0.0012 0.001 0.0012 0.0005
Cell Periphery 0.0018 0.0008 0.0006 0.0009 0.0002 0.0002 0.0009 0.0002 0.0003 0.0001 0.0001 0.0001 0.0002 0.0002 0.0001 0.0001 0.0002 0
Cytoplasm 0.5921 0.8997 0.7905 0.7719 0.7835 0.8677 0.6945 0.8761 0.8026 0.7629 0.6793 0.8375 0.7872 0.8708 0.8642 0.8151 0.7387 0.8874
Cytoplasmic Foci 0.0547 0.0189 0.0243 0.0237 0.0212 0.0247 0.0551 0.0245 0.0433 0.0694 0.0855 0.0405 0.0428 0.0323 0.0321 0.0359 0.0635 0.0299
Eisosomes 0.0011 0.0001 0.0002 0.0002 0 0.0001 0.0004 0 0.0001 0 0 0 0.0001 0 0 0.0001 0 0
Endoplasmic Reticulum 0.0148 0.0039 0.0096 0.0065 0.0062 0.0121 0.0112 0.0044 0.0041 0.0026 0.0037 0.0041 0.0351 0.0105 0.0051 0.0037 0.0037 0.0055
Endosome 0.0484 0.0238 0.0438 0.0591 0.0544 0.0327 0.0896 0.0372 0.0728 0.1103 0.1376 0.0683 0.061 0.0533 0.0564 0.0913 0.0864 0.0483
Golgi 0.0151 0.0015 0.012 0.0275 0.0039 0.005 0.0189 0.0033 0.01 0.0075 0.0149 0.0079 0.0097 0.0034 0.0051 0.0034 0.0025 0.0072
Lipid Particles 0.0214 0.0004 0.0115 0.0078 0.0001 0.0004 0.0065 0.0002 0.0009 0.0029 0.0065 0.0017 0.0023 0.0002 0.0005 0.0013 0.0001 0.0006
Mitochondria 0.005 0.0002 0.0044 0.0063 0.0011 0.0019 0.0187 0.002 0.0049 0.0016 0.0269 0.0075 0.0021 0.0007 0.0015 0.0009 0.0006 0.0012
None 0.1083 0.0422 0.0424 0.0576 0.1222 0.0338 0.028 0.0224 0.0226 0.0062 0.0037 0.0081 0.0194 0.0164 0.0165 0.0098 0.0265 0.0059
Nuclear Periphery 0.0036 0.0005 0.0011 0.0012 0.0003 0.0028 0.0058 0.0005 0.0008 0.0006 0.0043 0.0028 0.0079 0.0006 0.0009 0.0017 0.001 0.0005
Nucleolus 0.0014 0 0.0001 0.0001 0 0.0001 0.0004 0.0001 0.0002 0.0001 0.0006 0.0001 0.0003 0.0001 0 0.0003 0.0002 0
Nucleus 0.0013 0.0006 0.0008 0.0011 0.0004 0.0011 0.0073 0.0011 0.0012 0.0012 0.0039 0.0008 0.0034 0.0008 0.0008 0.0016 0.0014 0.0006
Peroxisomes 0.0246 0.0001 0.0132 0.0004 0.0001 0.0002 0.0108 0.0014 0.0043 0.0123 0.0046 0.0013 0.0024 0.0002 0.0004 0.0011 0.0003 0.0008
Punctate Nuclear 0.0078 0.0002 0.001 0.0003 0.0001 0.0007 0.0033 0.0004 0.0005 0.0023 0.0144 0.0006 0.0054 0.0005 0.0004 0.0035 0.0003 0.0003
Vacuole 0.0081 0.0033 0.0082 0.0137 0.0034 0.0033 0.0074 0.0056 0.0092 0.0119 0.0069 0.0037 0.0072 0.0052 0.0057 0.012 0.0639 0.003
Vacuole Periphery 0.001 0.0004 0.0012 0.0024 0.0011 0.0009 0.002 0.0011 0.0018 0.0031 0.0025 0.0015 0.0014 0.001 0.0013 0.0028 0.0067 0.0009

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 12.9215 13.6137 19.2856 14.3659 21.8391 12.4531 15.329 14.6393 13.4463 14.132
Translational Efficiency 3.211 2.5941 1.5689 2.0235 1.336 1.7882 1.5156 1.1998 1.2782 1.6518

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2387 1774 1458 1675 1600 1794 1477 3118 3987 3568 2935 4793

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 830.68 909.08 1098.20 1160.86 855.93 875.97 1052.57 1293.21 840.81 892.43 1075.24 1246.96
Standard Deviation 96.36 128.78 177.45 157.57 101.69 136.27 135.76 173.61 99.30 133.63 159.49 179.63
Intensity Change Log 2 — 0.130114 0.402776 0.482829 — 0.033389 0.298351 0.595392 — 0.081838 0.350727 0.541050

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000209 0.000212 0.001611 0.000546 0.000303 0.000325 0.000555 0.000273 0.000247 0.000269 0.001079 0.000368
Bud Neck 0.006333 0.003657 0.007538 0.002770 0.004188 0.006785 0.006828 0.002891 0.005472 0.005230 0.007181 0.002849
Bud Site 0.019389 0.022245 0.016595 0.033553 0.016022 0.026167 0.017367 0.021996 0.018038 0.024217 0.016984 0.026035
Cell Periphery 0.000135 0.000093 0.000223 0.000124 0.000178 0.000111 0.000084 0.000095 0.000153 0.000102 0.000153 0.000105
Cytoplasm 0.537293* 0.550583* 0.382387* 0.574395* 0.502234* 0.438252* 0.284213* 0.785431* 0.523224* 0.494103* 0.332982* 0.711680*
Cytoplasmic Foci 0.288890* 0.281787* 0.137016 0.106816 0.343262* 0.309853* 0.233174* 0.043456 0.310709* 0.295899* 0.185406* 0.065598
Eisosomes 0.000077 0.000031 0.000111 0.000009 0.000100 0.000069 0.000051 0.000006 0.000086 0.000050 0.000080 0.000007
Endoplasmic Reticulum 0.002263 0.001685 0.003780 0.000564 0.004565 0.002071 0.002573 0.000077 0.003187 0.001879 0.003173 0.000247
Endosome 0.026942 0.029846 0.051114 0.035171 0.026997 0.054992 0.127357 0.012840 0.026964 0.042489 0.089482 0.020644
Golgi 0.034449 0.022241 0.003281 0.010711 0.023472 0.027510 0.006393 0.008300 0.030044 0.024890 0.004847 0.009143
Lipid Particles 0.003483 0.001835 0.014133 0.002357 0.004189 0.005161 0.010053 0.001106 0.003766 0.003507 0.012079 0.001543
Mitochondria 0.004521 0.002616 0.000581 0.000794 0.002360 0.004966 0.000694 0.001073 0.003654 0.003798 0.000638 0.000976
Mitotic Spindle 0.000706 0.001791 0.056585 0.010560 0.002384 0.002602 0.030940 0.003193 0.001379 0.002199 0.043679 0.005767
None 0.001567 0.001005 0.002074 0.000234 0.000932 0.000451 0.001146 0.000133 0.001312 0.000726 0.001607 0.000168
Nuclear Periphery 0.000066 0.000051 0.001942 0.000143 0.000067 0.000500 0.000848 0.000034 0.000066 0.000277 0.001391 0.000072
Nuclear Periphery Foci 0.000205 0.000307 0.007057 0.000712 0.000583 0.000614 0.004994 0.000117 0.000357 0.000461 0.006019 0.000325
Nucleolus 0.000296 0.000196 0.002141 0.000258 0.000233 0.000645 0.002030 0.000257 0.000271 0.000422 0.002085 0.000257
Nucleus 0.007609 0.005235 0.086091 0.004542 0.008482 0.007068 0.023584 0.003189 0.007959 0.006157 0.054636 0.003662
Peroxisomes 0.002420 0.001974 0.007397 0.003208 0.001802 0.003237 0.008482 0.002684 0.002172 0.002609 0.007943 0.002867
Vacuole 0.053116 0.062658 0.216179* 0.204488* 0.050987 0.098782 0.234386* 0.107803 0.052262 0.080822 0.225341* 0.141591
Vacuole Periphery 0.010033 0.009952 0.002163 0.008048 0.006660 0.009836 0.004251 0.005047 0.008679 0.009894 0.003214 0.006096

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -0.17 -4.67 -3.65 -3.62 3.56 -0.55 -3.68 0.99 1.28 4.00 -1.00 -5.38 -2.87 -2.16 4.61
Bud Neck 6.42 -2.20 9.99 2.77 7.94 -6.81 -4.83 5.04 10.69 7.47 0.82 -4.08 10.88 9.75 10.78
Bud Site -2.44 1.29 -6.51 -4.74 -6.76 -6.85 -0.68 -4.87 3.31 -3.44 -6.52 0.83 -7.19 -0.51 -6.96
Cell Periphery 6.09 -3.43 1.81 -2.11 4.18 5.95 9.19 5.03 1.31 -0.69 8.09 1.31 4.76 0.24 2.98
Cytoplasm -1.52 15.80 -3.54 -2.03 -16.63 6.94 19.99 -35.53 -43.32 -53.89* 4.61 25.86 -30.94 -33.48 -51.93*
Cytoplasmic Foci 1.12 28.11 34.26 29.02 3.74 4.55 16.51 54.09 50.42 31.22* 3.06 28.89 66.67 58.69 27.58
Eisosomes 10.85 -0.99 16.92 13.70 7.05 4.56 7.54 16.24 17.47 10.99 9.33 2.66 23.71 21.54 10.29
Endoplasmic Reticulum 1.69 -2.33 6.27 5.14 7.54 3.47 3.08 6.97 6.25 7.96 3.63 0.88 9.66 8.47 11.19
Endosome -1.65 -6.22 -2.87 -1.39 3.52 -11.47 -19.76 10.45 18.52 23.51* -10.12 -19.51 6.22 14.93 22.30
Golgi 8.39 28.55 17.20 9.18 -6.71 -2.80 18.53 14.98 15.23 -3.20 4.92 33.15 24.12 17.96 -7.54
Lipid Particles 6.73 -9.35 2.68 -3.10 10.24 -1.64 -6.56 11.99 7.15 10.66 0.74 -11.45 11.06 6.16 15.16
Mitochondria 4.54 12.35 9.80 5.46 -0.86 -3.52 4.48 3.35 6.02 -2.45 -0.35 12.36 10.12 7.82 -2.67
Mitotic Spindle -2.24 -14.09 -5.49 -4.83 10.75 -0.09 -9.58 -0.82 -0.89 9.54 -1.39 -17.09 -5.47 -4.54 14.97
None 1.52 -0.26 5.46 2.51 2.79 1.77 -0.43 3.04 4.95 4.11 2.57 -0.25 6.52 3.65 4.30
Nuclear Periphery 0.56 -10.23 -2.35 -3.39 9.74 -1.76 -11.11 1.77 1.88 11.65 -1.70 -13.61 -0.69 1.60 13.56
Nuclear Periphery Foci -1.74 -12.82 -6.13 -4.89 11.63 -0.07 -8.62 2.90 2.88 9.62 -0.92 -15.30 -0.44 0.68 15.32
Nucleolus 2.05 -4.54 0.84 -1.61 4.61 -2.31 -4.22 -0.65 2.21 4.17 -1.69 -6.07 0.01 1.76 6.09
Nucleus 5.99 -18.60 4.91 -0.29 19.14 1.69 -9.34 10.20 4.61 12.31 3.91 -20.33 12.52 4.51 22.12
Peroxisomes 2.79 -4.69 -2.54 -4.20 3.81 -7.75 -10.86 -4.25 2.45 9.10 -3.51 -10.05 -3.87 -0.95 8.59
Vacuole -3.39 -27.26 -25.71 -23.34 2.65 -11.86 -29.66 -17.03 -3.13 18.51 -11.44 -40.16 -29.39 -18.20 16.81
Vacuole Periphery 0.12 17.18 3.73 3.10 -8.65 -4.14 5.19 3.34 7.71 -3.01 -2.19 15.80 7.06 8.23 -9.31
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Components••••••••••••••••actinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
Standard name
Human Ortholog
Description Unconventional prefoldin protein involved in translation initiation; required for correct assembly of RNAP I, II, and III in an Rpb5p-dependent manner; shuttles between nucleus and cytoplasm; mutants have inappropriate expression of nutrient sensitive genes due to translational derepression of Gcn4p transcription factor; diploid mutants show random budding; ortholog of human URI/RMP
Localization
Cell Percentages cytoplasm (98%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-4

Bud27

Bud27


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Bud27-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available