Standard name
Human Ortholog
Description GTPase-activating protein for yeast Rab family members; Ypt1p is the preferred in vitro substrate but also acts on Sec4p, Ypt31p and Ypt32p; involved in the regulation of ER to Golgi vesicle transport
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.1 0.09 0.05 0.09 0.13
Bud 0 0 0.05 0.08 0.05 0 0 0.06 0 0.09 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0
Cytoplasm 0.16 0.31 0.25 0.25 0.19 0.12 0.11 0.12 0.12 0.13 0.14 0.16 0.66 0.74 0.82 0.68 0.58 0.55 0 0.05 0 0.07 0 0.05
Endoplasmic Reticulum 0 0.12 0 0 0 0 0 0 0 0 0 0 0 0 0 0.11 0.07 0.08 0 0 0 0 0 0
Endosome 0 0.07 0.09 0.07 0.2 0 0 0 0 0 0 0 0.09 0.07 0.11 0.08 0.08 0.12 0.15 0.12 0.13 0.06 0.07 0.07
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0
Mitochondria 0.85 0.35 0.58 0.63 0.59 0.9 0.87 0.82 0.91 0.91 0.91 0.89 0.15 0.08 0 0.1 0.06 0 0.53 0.53 0.5 0.58 0.65 0.52
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0.07 0.13 0.09 0 0 0 0 0 0 0 0.08 0 0.05 0.06 0 0 0.1 0.05 0.1 0.05 0 0
SpindlePole 0.06 0 0.06 0.05 0 0 0 0.07 0 0 0 0 0 0 0 0 0.05 0.08 0 0 0 0 0 0
Vac/Vac Membrane 0 0.12 0 0 0.06 0.06 0 0 0 0 0 0 0 0.07 0 0.1 0.21 0.16 0 0.05 0 0.05 0.05 0.07
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 3 13 3 2 0 0 0 0 0 0 0 0 2 2 0 0 3 0 37 40 22 7 22 37
Bud 18 11 11 14 13 9 12 29 14 44 12 15 5 2 1 1 1 0 1 3 5 1 3 6
Bud Neck 1 1 0 0 0 0 0 2 0 0 0 0 0 0 0 2 0 2 5 7 6 2 5 11
Bud Site 3 2 1 1 0 1 3 6 6 3 1 8 0 0 0 1 0 0 – – – – – –
Cell Periphery 1 7 5 3 1 2 2 0 1 0 1 3 5 3 2 1 9 4 1 1 3 2 2 6
Cytoplasm 68 84 52 45 49 36 45 58 44 61 39 62 131 165 252 138 83 85 6 21 6 10 10 15
Endoplasmic Reticulum 16 32 7 3 7 8 5 2 1 2 3 1 7 9 3 23 10 13 2 6 4 2 4 8
Endosome 3 19 18 13 52 0 10 14 1 2 1 0 17 15 33 17 11 18 65 49 33 8 17 19
Golgi 0 3 0 1 0 0 0 1 0 0 0 0 2 0 1 4 3 1 22 15 8 5 1 7
Mitochondria 355 94 119 112 149 265 352 392 322 437 259 342 30 18 10 20 9 4 236 224 127 85 168 154
Nucleus 1 0 0 0 2 3 2 4 4 4 1 2 0 0 0 4 0 3 0 1 0 0 0 0
Nuclear Periphery 3 1 0 1 1 0 4 1 2 1 1 1 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 1 2 3 2 1 1 1 1 0 0 1 0 0 0 0 0 0 0 0 0
Peroxisomes 8 3 15 24 22 5 9 13 5 5 0 0 16 9 16 12 4 6 43 20 24 7 4 2
SpindlePole 24 4 12 9 11 4 11 35 9 17 7 7 1 4 1 3 7 13 5 5 3 3 3 2
Vac/Vac Membrane 15 31 7 5 14 17 17 20 9 19 5 7 8 16 10 20 30 25 14 22 6 7 11 19
Unique Cell Count 418 269 206 178 254 294 405 477 353 480 285 384 198 223 308 202 143 155 447 424 256 148 261 294
Labelled Cell Count 519 305 250 233 322 352 475 579 419 596 331 449 224 243 330 246 170 174 447 424 256 148 261 294


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.6 4.8 4.5 4.6 4.4 3.9 3.4 3.7 3.4 3.1 3.4 3.2 4.8 4.8 4.7 5.5 6.8 6.3 4.9 5.0 6.0
Std Deviation (1e-4) 0.4 0.7 1.3 2.4 1.6 1.5 1.5 1.4 1.1 1.1 1.3 1.3 0.9 1.4 1.3 1.6 2.2 1.9 1.2 1.0 1.1
Intensity Change (Log2) – – – 0.03 -0.02 -0.2 -0.38 -0.27 -0.39 -0.54 -0.41 -0.51 0.1 0.09 0.08 0.29 0.6 0.49 0.14 0.15 0.43


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 2.6 5.2 4.2

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.9035 0.177 -0.4783 -0.0099 -0.8232 -0.4361 2.619 2.7663 2.688 1.7428 2.5342 2.8896 3.4882 3.5678 3.5578 4.4756 3.1163 3.7908
Actin 0.0427 0.0136 0.0178 0.0043 0.0063 0.0192 0.034 0.0246 0.0473 0.0045 0.0372 0.0262 0.0427 0.0375 0.0269 0.0174 0.0474 0.0107
Bud 0.0009 0.0027 0.0015 0.0009 0.0029 0.001 0.0034 0.0052 0.0059 0.0047 0.0148 0.0076 0.0022 0.0033 0.0039 0.0031 0.0053 0.0008
Bud Neck 0.0054 0.0026 0.0341 0.0023 0.0155 0.0507 0.0092 0.004 0.0228 0.039 0.003 0.0133 0.0081 0.0022 0.0184 0.0057 0.0463 0.015
Bud Periphery 0.0022 0.0049 0.0042 0.0044 0.0092 0.0026 0.0069 0.0114 0.0147 0.0092 0.0328 0.0101 0.0061 0.0061 0.0114 0.0104 0.0073 0.0023
Bud Site 0.0141 0.0454 0.0152 0.001 0.003 0.0033 0.0205 0.0529 0.0404 0.0045 0.011 0.0031 0.0231 0.0393 0.0192 0.003 0.0046 0.0007
Cell Periphery 0.0031 0.0018 0.0019 0.0011 0.0013 0.002 0.003 0.0026 0.0047 0.0011 0.0015 0.0008 0.0051 0.0025 0.0023 0.003 0.0012 0.0008
Cytoplasm 0.072 0.1401 0.0734 0.0506 0.1516 0.1601 0.1088 0.1419 0.1399 0.044 0.0892 0.1232 0.1 0.1594 0.1437 0.1425 0.1602 0.1492
Cytoplasmic Foci 0.1513 0.192 0.1949 0.2368 0.2465 0.1987 0.2086 0.1972 0.1773 0.2136 0.1891 0.2202 0.1811 0.1625 0.1798 0.1648 0.2603 0.2014
Eisosomes 0.003 0.0008 0.0021 0.0006 0.0006 0.0013 0.0018 0.0009 0.0013 0.0004 0.0007 0.0005 0.001 0.0033 0.0007 0.001 0.0004 0.0005
Endoplasmic Reticulum 0.0072 0.0081 0.0049 0.0045 0.0043 0.0064 0.0107 0.0024 0.0039 0.0033 0.0029 0.0023 0.0164 0.0112 0.0088 0.004 0.0049 0.0087
Endosome 0.1342 0.105 0.1227 0.2097 0.1323 0.1117 0.1422 0.104 0.0821 0.0928 0.0819 0.0926 0.1747 0.1406 0.0998 0.1622 0.1121 0.1315
Golgi 0.0403 0.031 0.0341 0.0674 0.0465 0.0356 0.0404 0.0328 0.0292 0.0365 0.0428 0.0333 0.035 0.0305 0.0307 0.0449 0.0355 0.0298
Lipid Particles 0.0553 0.018 0.0244 0.0084 0.067 0.0286 0.0272 0.011 0.0103 0.0116 0.014 0.0173 0.0501 0.0188 0.0206 0.007 0.0131 0.0346
Mitochondria 0.0608 0.0591 0.0817 0.0689 0.0388 0.0468 0.0394 0.0288 0.0407 0.0782 0.1006 0.0615 0.0354 0.0424 0.0473 0.1239 0.0529 0.0477
None 0.3293 0.324 0.3004 0.1739 0.1915 0.2385 0.2455 0.3135 0.2784 0.2512 0.2274 0.2917 0.2412 0.2883 0.307 0.2658 0.1688 0.3008
Nuclear Periphery 0.0022 0.0018 0.0016 0.0008 0.0027 0.0016 0.0077 0.0012 0.0012 0.0027 0.0032 0.0009 0.0118 0.0016 0.0045 0.0008 0.0033 0.0021
Nucleolus 0.0024 0.0018 0.0045 0.001 0.0014 0.002 0.0022 0.0015 0.0011 0.0019 0.0013 0.0009 0.0014 0.0011 0.0016 0.0006 0.0012 0.0005
Nucleus 0.002 0.0022 0.0043 0.001 0.002 0.0021 0.004 0.0027 0.0019 0.0028 0.002 0.0027 0.0062 0.0018 0.0027 0.0011 0.0023 0.0011
Peroxisomes 0.0483 0.0326 0.0553 0.0998 0.0556 0.0723 0.0651 0.0454 0.0854 0.1747 0.1258 0.0788 0.033 0.0346 0.0497 0.0257 0.0515 0.0509
Punctate Nuclear 0.0094 0.0024 0.0081 0.0495 0.0115 0.0058 0.0081 0.0079 0.0036 0.0045 0.0095 0.0068 0.0048 0.0028 0.0078 0.0016 0.0116 0.002
Vacuole 0.0119 0.0077 0.0104 0.0097 0.0066 0.0072 0.0089 0.0069 0.0061 0.0144 0.0057 0.0047 0.0165 0.0083 0.0108 0.008 0.0078 0.0064
Vacuole Periphery 0.0023 0.0023 0.0024 0.0033 0.0029 0.0022 0.0024 0.0014 0.0019 0.0044 0.0036 0.0015 0.0039 0.0019 0.0025 0.0036 0.002 0.0025

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 7.3856 8.5829 12.2479 15.7256 11.9696 13.2867 9.5126 15.5441 12.7 12.9576
Translational Efficiency 0.7273 0.6855 0.6929 0.5464 0.6045 0.4835 0.7122 0.4848 0.632 0.5519

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2058 1934 401 1404 478 1427 1457 1672 2536 3361 1858 3076

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 666.34 753.06 879.83 807.86 662.45 687.68 898.21 856.68 665.61 725.30 894.24 834.40
Standard Deviation 70.85 106.61 96.71 118.56 83.19 87.86 108.32 123.62 73.35 104.22 106.19 123.75
Intensity Change Log 2 — 0.176506 0.400966 0.277847 — 0.053926 0.439241 0.370945 — 0.116697 0.420175 0.325010

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.006302 0.012939 0.029856 0.102291* 0.005163 0.010722 0.031667 0.063672 0.006087 0.011998 0.031277 0.081299
Bud Neck 0.041871 0.015334 0.017863 0.020723 0.025377 0.018129 0.019796 0.023727 0.038762 0.016521 0.019379 0.022356
Bud Site 0.027582 0.035726 0.057072 0.111698* 0.014577 0.027772 0.052738 0.115078* 0.025131 0.032348 0.053673 0.113535*
Cell Periphery 0.000485 0.000345 0.000381 0.000451 0.000404 0.000498 0.000256 0.000532 0.000470 0.000410 0.000283 0.000495
Cytoplasm 0.109659* 0.039997 0.077151 0.141870* 0.123357* 0.040052 0.175727* 0.178594* 0.112241* 0.040020 0.154452* 0.161832*
Cytoplasmic Foci 0.298680* 0.223400* 0.208970* 0.195206* 0.396561* 0.206663* 0.247533* 0.180016* 0.317129* 0.216294* 0.239211* 0.186949*
Eisosomes 0.001054 0.000567 0.000306 0.000709 0.000594 0.000704 0.000292 0.000644 0.000967 0.000625 0.000295 0.000674
Endoplasmic Reticulum 0.003953 0.000796 0.011512 0.004505 0.002513 0.001028 0.006261 0.003378 0.003681 0.000895 0.007394 0.003892
Endosome 0.066530 0.086241 0.048495 0.031807 0.046353 0.091972 0.042852 0.045692 0.062727 0.088674 0.044070 0.039355
Golgi 0.083852 0.204188* 0.018201 0.042220 0.050779 0.184705* 0.019980 0.032827 0.077618 0.195916* 0.019596 0.037114
Lipid Particles 0.034029 0.008395 0.025226 0.007750 0.042881 0.010681 0.013635 0.008848 0.035698 0.009366 0.016137 0.008347
Mitochondria 0.186700* 0.222298* 0.004844 0.022993 0.071141 0.237404* 0.009044 0.027170 0.164919* 0.228711* 0.008138 0.025263
Mitotic Spindle 0.002626 0.010429 0.055965 0.025117 0.002157 0.010063 0.036488 0.037986 0.002538 0.010274 0.040692 0.032112
None 0.000837 0.000442 0.000632 0.000503 0.002946 0.001951 0.001473 0.001677 0.001235 0.001082 0.001291 0.001141
Nuclear Periphery 0.000304 0.000071 0.001674 0.000164 0.000565 0.000107 0.001048 0.000440 0.000353 0.000086 0.001183 0.000314
Nuclear Periphery Foci 0.000309 0.000193 0.005925 0.001071 0.000821 0.000163 0.003876 0.001589 0.000406 0.000180 0.004318 0.001353
Nucleolus 0.000767 0.000385 0.002646 0.000297 0.003994 0.001054 0.001518 0.000863 0.001375 0.000669 0.001762 0.000605
Nucleus 0.013604 0.002102 0.076545 0.022697 0.042565 0.004085 0.050997 0.024416 0.019063 0.002944 0.056511 0.023631
Peroxisomes 0.071784 0.107327* 0.267152* 0.198235* 0.138165* 0.127853* 0.170055* 0.128241* 0.084296 0.116042* 0.191011* 0.160189*
Vacuole 0.039768 0.020079 0.087073 0.068244 0.023208 0.016893 0.112552 0.120575 0.036647 0.018726 0.107053 0.096689
Vacuole Periphery 0.009303 0.008747 0.002511 0.001448 0.005881 0.007503 0.002211 0.004035 0.008658 0.008219 0.002276 0.002854

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -4.89 -6.94 -18.25 -16.66 -11.66 -3.62 -9.56 -13.66 -12.41 -6.99 -5.82 -11.73 -22.51 -20.42 -13.46
Bud Neck 11.03 8.15 8.55 -3.52 -1.38 1.90 1.42 0.41 -2.95 -2.07 10.82 8.90 7.95 -4.84 -2.11
Bud Site -3.30 -5.62 -17.60 -15.32 -8.12 -4.43 -10.26 -20.20 -17.92 -12.36 -3.77 -9.85 -25.66 -23.25 -15.52
Cell Periphery 2.10 1.27 0.54 -2.45 -1.14 -0.76 1.60 -1.45 -0.56 -6.75 0.92 3.48 -0.59 -1.98 -7.37
Cytoplasm 17.15 3.57 -6.57 -19.53 -7.87 9.82 -5.83 -6.60 -24.69 -0.92 20.08 -8.24 -11.78 -31.55 -1.95
Cytoplasmic Foci 12.56 9.82 16.89 5.77 1.58 17.09 14.39 20.27 5.68 9.33 19.64 14.95 26.09 8.59 8.84
Eisosomes 4.27 9.87 4.57 -1.16 -8.02 -1.11 4.43 0.04 1.17 -4.53 4.00 11.16 4.70 -0.04 -8.13
Endoplasmic Reticulum 5.67 -6.84 -1.01 -6.76 6.17 2.17 -4.71 -1.49 -5.43 4.24 5.80 -5.94 -0.74 -9.02 5.99
Endosome -5.61 4.16 13.05 17.36 3.99 -8.26 1.51 0.98 11.91 -0.82 -8.79 8.06 10.99 20.02 2.11
Golgi -21.54* 18.91 12.56 30.71* -6.49 -19.02 7.08 4.43 27.17 -5.17 -27.17* 22.88 15.78 41.60 -8.58
Lipid Particles 14.10 2.75 14.67 0.54 7.38 7.73 7.04 8.23 1.34 3.92 15.86 10.90 16.62 1.15 7.70
Mitochondria -4.40 33.85* 29.40* 31.89* -8.72 -17.30 9.56 6.87 28.61* -7.57 -9.79 33.88 29.71 42.72* -10.26
Mitotic Spindle -7.30 -7.76 -9.19 -5.70 4.35 -5.13 -11.57 -12.27 -8.67 -0.01 -8.30 -13.75 -15.05 -10.50 2.93
None 2.84 0.73 2.05 -0.41 1.10 0.85 1.68 1.45 0.37 -0.28 0.36 -0.11 0.48 -0.01 0.53
Nuclear Periphery 3.06 -3.21 1.05 -6.32 3.44 4.67 -4.09 0.23 -6.94 4.75 4.10 -6.33 -0.56 -8.91 6.49
Nuclear Periphery Foci 2.03 -6.50 -3.58 -4.03 5.39 4.10 -5.81 -2.23 -4.54 4.13 4.29 -8.75 -4.86 -6.01 6.26
Nucleolus 2.81 -2.90 3.57 0.51 3.47 2.55 2.08 2.85 0.41 2.58 2.37 -1.92 2.78 0.22 4.98
Nucleus 11.98 -7.94 -7.35 -14.39 6.17 9.46 -3.19 2.89 -12.47 8.04 14.02 -13.95 -6.00 -19.11 10.50
Peroxisomes -7.12 -12.24 -16.54 -10.36 4.10 0.90 -1.22 2.06 1.70 4.61 -7.36 -13.81 -13.12 -6.24 3.58
Vacuole 8.15 -7.80 -11.54 -16.35 0.90 2.07 -21.19 -21.83 -24.63 -0.83 9.14 -21.20 -22.84 -29.19 1.87
Vacuole Periphery 0.68 11.08 15.50 11.41 2.46 -1.47 3.92 2.19 5.29 -3.40 0.67 13.30 12.32 11.22 -1.62
Standard name
Human Ortholog
Description GTPase-activating protein for yeast Rab family members; Ypt1p is the preferred in vitro substrate but also acts on Sec4p, Ypt31p and Ypt32p; involved in the regulation of ER to Golgi vesicle transport
Localization
Cell Percentages mitochondrion (27%)
Cell Cycle Regulation No
Subcompartmental Group mito-1

Gyp8

Gyp8


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Gyp8-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available