Standard name
Human Ortholog
Description Part of evolutionarily-conserved CCR4-NOT regulatory complex; contains single ABC-type ATPase domain but no transmembrane domain; interacts with several subunits of Mediator
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.99 0.98 0.97 0.96 0.85 0.89 0.81 0.75 0.75 0.83 0.78 0.71 0.98 0.98 0.98 0.94 0.94 0.91 0.98 0.96 0.94 0.89 0.89 0.89
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0.15 0.08 0.14 0.2 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0.05 0 0.14 0.07 0.15 0.21 0.09 0.07 0.12 0.12 0 0 0 0.05 0.06 0.15 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 1 0 0 1 2 1 1 0 2 0 0 0 0 0 0 0 0 1 0 0 0
Bud 0 0 0 0 2 0 0 4 4 1 1 2 0 0 0 0 0 0 1 0 2 1 6 7
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 4 0 3 5 3 3 4 9 12 9 2 11 0 1 0 4 5 5 0 0 0 0 0 0
Cytoplasm 396 383 383 222 157 275 321 270 263 254 161 210 217 305 272 326 289 279 389 372 375 129 257 318
Endoplasmic Reticulum 0 4 2 2 2 7 9 3 11 9 0 2 0 2 2 10 15 11 0 1 1 1 2 1
Endosome 0 0 0 0 0 0 1 1 1 0 0 1 0 0 0 1 1 1 0 1 0 0 1 2
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 4 2 0 0 0 0 0 0
Mitochondria 0 0 0 1 6 4 8 12 54 24 28 60 1 0 2 2 2 2 0 2 2 1 1 4
Nucleus 2 1 1 3 3 6 11 3 3 10 6 11 0 2 1 3 7 3 0 5 3 4 7 6
Nuclear Periphery 0 0 0 0 0 0 0 1 0 0 1 0 0 0 0 0 0 0 0 0 1 1 0 1
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1
Vac/Vac Membrane 5 3 18 5 26 23 59 77 32 21 25 35 7 6 0 18 20 46 0 3 4 2 3 4
Unique Cell Count 399 391 396 231 185 308 398 359 349 306 206 296 222 310 277 345 308 306 396 390 402 145 288 360
Labelled Cell Count 407 391 407 239 199 318 414 382 381 329 224 334 225 316 277 364 343 349 396 390 402 145 288 360


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 17.5 12.9 14.5 12.9 9.4 10.6 9.8 9.9 8.1 8.7 8.8 7.6 13.7 13.9 12.8 13.0 16.3 16.3 12.3 13.7 14.6
Std Deviation (1e-4) 3.0 2.6 2.4 2.1 2.2 2.0 1.8 1.9 1.6 2.0 2.4 2.1 2.7 2.2 2.4 2.9 3.5 3.6 3.7 3.9 3.9
Intensity Change (Log2) – – – -0.17 -0.62 -0.45 -0.57 -0.55 -0.84 -0.74 -0.72 -0.93 -0.08 -0.06 -0.18 -0.16 0.17 0.17 -0.23 -0.08 0.01


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 1.9 2.6 2.2 0 2.7 0 0 0 0 0 0
Cytoplasm -0.4 -5.2 -4.0 -7.1 -8.6 -8.6 -6.2 -7.3 -9.6 0.7 1.4 1.2 -1.5 -1.8 -3.1
Endoplasmic Reticulum 0 0 0 2.1 0 2.8 0 0 0 0 0 0 2.6 3.7 3.0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 3.7 8.1 5.7 7.5 9.4 0 0 0 0 0 0
Nucleus 0 0 0 2.9 0 0 0 0 3.5 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -1.5 4.0 1.6 4.9 7.0 2.5 1.3 3.4 3.6 -0.8 -1.9 -3.6 0.4 1.1 4.8

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 11.0978 14.2003 12.8804 11.8688 10.2773 12.3766 10.0012 15.4133 14.6744 13.3685 11.764 14.7 10.359 13.6292 12.0052 12.1335 10.4216 12.2487
Actin 0.0453 0.0002 0.0043 0.0004 0.0036 0.0019 0.0761 0.0006 0.0017 0.0005 0.0638 0.0003 0.0165 0.0003 0.0102 0.0003 0.0111 0.0109
Bud 0.0006 0.0001 0.0005 0.0002 0.0002 0.0001 0.0034 0.0001 0.0001 0.0001 0.0072 0.0001 0.001 0.0001 0.0004 0.0001 0.0009 0.0006
Bud Neck 0.0028 0.0002 0.0008 0.0003 0.0005 0.0011 0.0015 0.0001 0.0003 0.0004 0.0007 0.0005 0.0014 0.0002 0.0005 0.0005 0.0015 0.001
Bud Periphery 0.0009 0 0.0004 0.0002 0.0002 0.0001 0.0033 0 0 0 0.0166 0 0.0008 0 0.0001 0 0.0012 0.0004
Bud Site 0.0032 0.0002 0.0041 0.0003 0.0004 0.0002 0.0089 0.0001 0.0001 0.0001 0.0069 0 0.0025 0.0002 0.001 0 0.001 0.0002
Cell Periphery 0.0009 0 0.0001 0.0001 0.0001 0.0001 0.0002 0 0 0 0.0003 0 0.0001 0 0 0 0.0001 0
Cytoplasm 0.7632 0.987 0.9337 0.8803 0.694 0.9334 0.5832 0.9925 0.9804 0.9815 0.6557 0.9548 0.7824 0.9798 0.9244 0.9636 0.8652 0.94
Cytoplasmic Foci 0.0262 0.0008 0.0076 0.0041 0.0572 0.0052 0.0713 0.0008 0.0047 0.0015 0.0166 0.0019 0.0328 0.0013 0.0129 0.002 0.022 0.0077
Eisosomes 0.0003 0 0 0 0.0001 0 0.0003 0 0 0 0.0003 0 0.0001 0 0 0 0 0
Endoplasmic Reticulum 0.008 0.0007 0.0023 0.0012 0.0028 0.0016 0.0032 0.0007 0.0005 0.0005 0.0029 0.0013 0.0137 0.0005 0.0015 0.0007 0.0023 0.0012
Endosome 0.0323 0.0002 0.0112 0.0676 0.1042 0.012 0.0567 0.0001 0.0004 0.0003 0.0523 0.0142 0.0553 0.0003 0.0076 0.0004 0.013 0.002
Golgi 0.012 0 0.0023 0.0131 0.0426 0.011 0.0484 0 0.0002 0 0.0075 0.0062 0.0075 0 0.0016 0 0.016 0.0005
Lipid Particles 0.0087 0 0.0061 0.0061 0.0221 0.0039 0.0446 0 0 0 0.0022 0 0.0018 0 0.002 0 0.0011 0.0003
Mitochondria 0.0122 0.0001 0.0065 0.0088 0.0459 0.0068 0.0417 0 0.0002 0.0001 0.1159 0.0032 0.0078 0.0001 0.0012 0.0001 0.0364 0.0003
None 0.0124 0.0013 0.0016 0.0006 0.0008 0.0012 0.0033 0.0014 0.001 0.0018 0.0026 0.0012 0.0074 0.0049 0.0019 0.0019 0.0026 0.0022
Nuclear Periphery 0.0133 0.0009 0.003 0.0012 0.0027 0.0019 0.002 0.0005 0.0007 0.0014 0.0071 0.0012 0.0111 0.0009 0.0054 0.0016 0.0019 0.0015
Nucleolus 0.0006 0 0.0001 0.0001 0.0001 0 0.0003 0 0 0 0.0005 0 0.0002 0 0.0001 0 0.0001 0
Nucleus 0.0263 0.008 0.0097 0.0063 0.0121 0.0155 0.0033 0.0027 0.0079 0.0111 0.0273 0.0133 0.0369 0.0108 0.0202 0.0275 0.0158 0.0288
Peroxisomes 0.0204 0 0.0035 0.0005 0.0061 0.0028 0.0396 0 0.0013 0 0.0051 0 0.007 0 0.0043 0.0001 0.0051 0.0007
Punctate Nuclear 0.0022 0.0001 0.0013 0.0002 0.001 0.0005 0.0043 0.0001 0.0004 0.0006 0.0036 0.0005 0.0039 0.0002 0.0038 0.0008 0.0011 0.0014
Vacuole 0.0057 0.0002 0.0007 0.0055 0.002 0.0004 0.0026 0.0001 0.0001 0.0002 0.0033 0.0005 0.0065 0.0002 0.0005 0.0002 0.001 0.0002
Vacuole Periphery 0.0023 0 0.0004 0.0028 0.0013 0.0002 0.0017 0 0 0.0001 0.0016 0.0005 0.0031 0 0.0003 0.0001 0.0005 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 19.6584 18.1403 15.9848 24.0422 26.3101 25.3516 28.0856 29.4009 29.8863 24.9484
Translational Efficiency 1.8913 2.199 2.3671 1.6897 1.3022 1.3648 1.6243 1.5231 1.3628 1.5208

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1462 1230 1680 1809 2804 1577 2808 1529 4266 2807 4488 3338

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1172.94 1367.09 1108.71 953.66 1377.76 1439.25 1075.48 964.96 1307.57 1407.63 1087.92 958.84
Standard Deviation 210.05 214.05 172.46 174.14 210.79 227.04 172.57 170.92 231.90 224.32 173.27 172.77
Intensity Change Log 2 — 0.220979 -0.081247 -0.298582 — 0.062993 -0.357344 -0.513784 — 0.137797 -0.223795 -0.410824

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000262 0.000160 0.000455 0.001285 0.000094 0.000143 0.000055 0.000424 0.000152 0.000150 0.000205 0.000891
Bud Neck 0.000427 0.000422 0.001973 0.008319 0.000464 0.000688 0.001191 0.006450 0.000452 0.000572 0.001484 0.007463
Bud Site 0.003011 0.002158 0.006029 0.040096 0.001369 0.003682 0.003838 0.026869 0.001932 0.003014 0.004658 0.034037
Cell Periphery 0.000082 0.000061 0.000081 0.000116 0.000028 0.000033 0.000032 0.000105 0.000047 0.000045 0.000050 0.000111
Cytoplasm 0.864550* 0.862045* 0.712632* 0.616602* 0.855403* 0.854038* 0.809446* 0.648597* 0.858538* 0.857546* 0.773206* 0.631258*
Cytoplasmic Foci 0.028832 0.011015 0.012795 0.014285 0.010875 0.014010 0.007604 0.017241 0.017029 0.012698 0.009547 0.015639
Eisosomes 0.000005 0.000002 0.000015 0.000025 0.000002 0.000002 0.000005 0.000023 0.000003 0.000002 0.000009 0.000024
Endoplasmic Reticulum 0.001076 0.000292 0.001236 0.000991 0.000260 0.000222 0.000244 0.001183 0.000540 0.000253 0.000615 0.001079
Endosome 0.000780 0.000603 0.001989 0.006585 0.000331 0.001118 0.000841 0.004879 0.000485 0.000893 0.001271 0.005804
Golgi 0.001194 0.000752 0.000273 0.004889 0.000373 0.001035 0.000170 0.001849 0.000654 0.000911 0.000209 0.003497
Lipid Particles 0.000510 0.000179 0.000785 0.000697 0.000180 0.000256 0.000252 0.000723 0.000293 0.000222 0.000451 0.000709
Mitochondria 0.000467 0.000878 0.000223 0.002399 0.000185 0.000198 0.000335 0.000952 0.000281 0.000496 0.000293 0.001736
Mitotic Spindle 0.002647 0.001920 0.006072 0.057827 0.002772 0.006889 0.010256 0.036193 0.002730 0.004712 0.008690 0.047917
None 0.006597 0.011008 0.007146 0.007682 0.007789 0.009040 0.005819 0.006277 0.007380 0.009903 0.006316 0.007039
Nuclear Periphery 0.000110 0.000063 0.000639 0.000858 0.000086 0.000094 0.000382 0.001819 0.000094 0.000081 0.000478 0.001298
Nuclear Periphery Foci 0.003801 0.002430 0.002541 0.001332 0.000986 0.001363 0.000659 0.002002 0.001950 0.001831 0.001363 0.001639
Nucleolus 0.000117 0.000150 0.000287 0.000295 0.000094 0.000116 0.000185 0.000613 0.000102 0.000131 0.000223 0.000440
Nucleus 0.071419 0.097483 0.162915* 0.129289 0.112187 0.096550 0.111255 0.152080* 0.098215 0.096959 0.130593* 0.139729*
Peroxisomes 0.000142 0.000205 0.000457 0.001120 0.000171 0.000363 0.000104 0.001203 0.000161 0.000294 0.000236 0.001158
Vacuole 0.012668 0.007844 0.081296 0.101535 0.006110 0.009875 0.046766 0.088919 0.008358 0.008985 0.059692 0.095756
Vacuole Periphery 0.001303 0.000329 0.000160 0.003773 0.000242 0.000285 0.000561 0.001598 0.000605 0.000305 0.000411 0.002776

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 0.94 -1.02 -2.23 -3.15 -0.97 -1.30 0.54 -4.07 -3.13 -5.52 -0.34 -0.88 -3.92 -4.01 -2.97
Bud Neck -0.76 -5.60 -8.79 -8.63 -4.92 -1.88 -10.70 -7.03 -6.53 -4.90 -1.90 -9.99 -11.21 -10.74 -7.37
Bud Site 1.13 -2.96 -7.29 -7.59 -5.54 -2.79 -4.06 -6.54 -4.75 -5.30 -2.31 -5.03 -10.03 -8.69 -8.03
Cell Periphery 0.98 -0.08 -0.37 -2.58 -0.70 -1.60 -2.18 -13.53 -10.45 -12.97 -0.09 -0.83 -6.41 -8.08 -9.76
Cytoplasm 1.59 31.92 47.52 45.88 14.67 1.58 28.84 43.67 39.59 22.11 2.02 42.36 65.35 60.42 28.06
Cytoplasmic Foci 8.78 9.23 9.81 0.84 0.85 -2.84 3.41 -1.76 1.41 -5.16 3.62 9.12 6.15 1.90 -3.73
Eisosomes 2.46 -11.65 -20.87 -24.42* -8.83 -3.04 -16.13 -24.96 -24.20 -19.48 -0.13 -18.11 -33.74 -34.52* -21.45
Endoplasmic Reticulum 3.88 -1.73 -1.33 -10.02 0.69 0.42 -2.19 -13.11 -13.53 -12.75 3.60 -2.73 -9.00 -16.41 -8.70
Endosome 0.82 -7.25 -4.58 -4.42 -1.08 -2.50 -4.33 -4.87 -2.31 -3.63 -1.92 -8.29 -7.08 -4.48 -4.06
Golgi 1.06 3.25 0.05 -0.76 -1.91 -1.54 2.47 -0.89 0.90 -2.16 -0.72 4.02 -0.80 -0.10 -2.63
Lipid Particles 1.87 -2.35 -3.80 -18.28 -2.53 -1.92 -10.45 -16.63 -10.56 -11.18 0.60 -5.39 -12.31 -18.76 -10.41
Mitochondria -0.48 1.04 -2.35 -0.31 -5.30 -0.10 -1.17 -4.33 -4.99 -3.51 -0.58 -0.01 -4.78 -1.19 -6.04
Mitotic Spindle 0.57 -2.91 -8.98 -9.17 -7.45 -2.21 -4.21 -6.92 -4.73 -4.36 -1.64 -5.09 -11.28 -9.74 -8.43
None -3.01 0.56 -0.57 3.10 -1.54 -1.26 5.93 3.56 3.86 -1.56 -3.15 3.82 1.70 4.60 -2.57
Nuclear Periphery 1.24 -19.06 -14.71 -15.40 -7.60 -1.81 -25.22 -5.58 -5.50 -3.18 0.14 -31.79 -11.69 -11.72 -6.52
Nuclear Periphery Foci 2.80 3.15 8.39 6.48 6.51 -2.36 1.41 -4.18 -0.72 -5.34 -0.46 2.95 3.86 3.74 0.92
Nucleolus -0.66 -3.70 -7.67 -4.25 -1.56 -1.23 -4.54 -2.03 -1.86 -0.98 -1.23 -5.87 -4.21 -3.61 -1.71
Nucleus -7.77 -25.68 -24.33 -16.53 1.60 4.02 -10.59 -16.99 -18.97 -9.58 -0.65 -23.93 -26.82 -25.19 -6.73
Peroxisomes -0.73 -1.57 -2.26 -0.42 0.06 -1.26 0.78 -1.46 -0.44 -2.30 -1.35 -0.33 -2.12 -0.57 -2.06
Vacuole 2.02 -23.66 -35.70 -36.25 -13.08 -6.18 -31.07 -32.25 -30.64 -13.76 -4.03 -38.92 -48.48 -47.27 -20.13
Vacuole Periphery 1.60 1.92 0.55 -2.00 -2.94 -0.17 -0.14 -1.31 -1.24 -1.02 1.33 1.54 -0.53 -2.43 -2.63
Standard name
Human Ortholog
Description Part of evolutionarily-conserved CCR4-NOT regulatory complex; contains single ABC-type ATPase domain but no transmembrane domain; interacts with several subunits of Mediator
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-7

Caf16

Caf16


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Caf16-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available