Standard name
Human Ortholog
Description Protein kinase involved in cell proliferation in response to nutrients; glucose-repressible; involved in signal transduction during cell proliferation in response to nutrients, specifically the establishment of stationary phase; identified as a regulator of IME2; phosphorylates Igo1p and Igo2p; substrate of Pho80p-Pho85p kinase
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.98 0.95 0.98 0.99 0.98 0.95 0.93 0.89 0.86 0.84 0.8 0.81 0.99 0.99 0.99 0.97 0.98 0.99 0.87 0.84 0.78 0.92 0.85 0.85
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.05 0 0.07 0 0.17 0.22 0.21 0.34 0.37 0.41 0.39 0 0 0 0 0 0 0 0 0.1 0 0 0.05
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 3 2 2 6 7
Bud 0 0 0 3 1 2 9 12 4 10 5 5 0 0 9 0 0 0 0 1 3 3 19 10
Bud Neck 1 0 3 1 3 1 7 7 1 0 1 0 0 1 0 0 1 0 0 0 1 1 2 2
Bud Site 0 0 0 0 1 0 0 0 2 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 0 2 4 10 1 7 6 4 9 5 7 2 2 8 12 9 12 0 0 2 0 2 2
Cytoplasm 201 179 303 210 524 410 618 508 332 457 267 340 274 479 528 374 291 263 181 163 217 284 487 382
Endoplasmic Reticulum 1 1 1 0 2 0 0 1 1 2 0 0 1 1 3 19 10 14 1 0 1 0 2 2
Endosome 0 0 0 0 1 0 4 1 0 0 0 0 0 1 0 0 1 1 3 1 3 0 7 3
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 1 2 3 0 1 3 0
Mitochondria 1 9 6 15 23 74 146 120 130 201 136 162 1 0 7 6 1 2 7 7 28 4 17 20
Nucleus 0 0 1 0 1 0 0 4 2 4 0 2 0 1 1 4 1 2 0 1 1 0 1 1
Nuclear Periphery 0 1 0 0 1 0 0 0 1 0 0 0 0 0 2 0 1 0 1 0 0 0 0 0
Nucleolus 0 1 0 0 0 0 0 0 0 1 0 0 0 1 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 2 0 1 1 0 2 3 3 2 0 0 0 0 0 0 0 2 2 1 2 7 5
Vac/Vac Membrane 1 4 0 0 5 1 5 2 1 2 0 4 0 2 0 8 7 2 0 2 2 2 4 5
Unique Cell Count 205 189 309 212 537 431 662 569 387 542 334 420 276 486 534 384 296 267 209 195 278 308 575 449
Labelled Cell Count 207 195 318 233 573 490 796 663 481 689 416 520 278 489 558 425 322 297 209 195 278 308 575 449


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.8 4.0 3.4 3.0 3.6 3.2 3.3 3.5 2.9 3.0 2.8 2.8 4.6 4.7 4.2 7.0 7.4 7.3 4.6 4.6 5.2
Std Deviation (1e-4) 0.8 1.1 1.2 1.2 1.6 1.2 1.3 1.5 0.9 1.3 0.7 0.9 1.3 1.2 1.1 1.3 1.5 1.4 1.1 1.4 1.6
Intensity Change (Log2) – – – -0.15 0.12 -0.05 -0.03 0.07 -0.22 -0.14 -0.26 -0.25 0.45 0.5 0.32 1.06 1.14 1.12 0.47 0.45 0.62


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.6547 1.4287 1.4256 0.9191 1.1323 1.3348 2.3972 2.1982 2.1311 1.9051 1.8011 2.2551 2.4151 3.118 2.4689 3.5903 2.557 2.7127
Actin 0.0529 0.0002 0.028 0.0218 0.0365 0.0223 0.0642 0.0022 0.0224 0.0006 0.0121 0.0015 0.0001 0.0001 0.0044 0.0002 0.0008 0.0094
Bud 0.0003 0.0027 0.0013 0.0003 0.0008 0.0002 0.0139 0.0111 0.0112 0.0123 0.0017 0.0024 0 0 0.0005 0 0.0001 0.0003
Bud Neck 0.0016 0.0003 0.0008 0.0009 0.0085 0.0006 0.0104 0.0024 0.0043 0.0014 0.0003 0.0011 0 0.0001 0.0003 0.0001 0.0002 0.0003
Bud Periphery 0.0004 0.0007 0.001 0.0004 0.0017 0.0003 0.0069 0.0051 0.0037 0.0079 0.0053 0.0015 0 0 0.0009 0 0.0002 0.0005
Bud Site 0.0051 0.0042 0.0062 0.0008 0.0296 0.0002 0.014 0.0287 0.0157 0.0015 0.0005 0.0015 0.0001 0.0003 0.0011 0.0001 0.0001 0.0001
Cell Periphery 0.0004 0.0005 0.0006 0.0002 0.0003 0.0001 0.0014 0.0007 0.0004 0.0002 0.0004 0.0001 0.0001 0.0002 0.0003 0.0001 0.0001 0.0001
Cytoplasm 0.2859 0.5373 0.4125 0.3584 0.2247 0.5547 0.1468 0.2366 0.1752 0.153 0.112 0.2367 0.2455 0.3394 0.3721 0.3334 0.3116 0.3893
Cytoplasmic Foci 0.0199 0.0126 0.0062 0.0137 0.0339 0.0056 0.0275 0.0066 0.0136 0.0051 0.0084 0.0049 0.0025 0.0035 0.0083 0.0035 0.008 0.0049
Eisosomes 0.0009 0.0001 0.0006 0.0002 0.0004 0.0002 0.0008 0.0001 0.0002 0.0001 0.001 0.0001 0.0001 0.0001 0.0002 0.0001 0.0001 0.0001
Endoplasmic Reticulum 0.0049 0.0049 0.0028 0.003 0.0035 0.0048 0.0063 0.0008 0.0013 0.0004 0.0025 0.0006 0.0009 0.0037 0.0031 0.0015 0.0022 0.0024
Endosome 0.0121 0.0026 0.0042 0.012 0.022 0.0041 0.0422 0.0018 0.0071 0.0015 0.002 0.0028 0.0003 0.0015 0.0043 0.0016 0.0015 0.0017
Golgi 0.0041 0.0002 0.0034 0.0065 0.0168 0.0017 0.0123 0.0002 0.0025 0.0001 0.0008 0.0008 0 0.0001 0.0012 0.0001 0.0002 0.0007
Lipid Particles 0.0107 0.0004 0.0018 0.0112 0.0286 0.0008 0.0251 0.0002 0.0037 0 0.0054 0.0023 0.0001 0.0002 0.0014 0.0001 0.0004 0.0005
Mitochondria 0.0025 0.0004 0.0137 0.0125 0.0114 0.001 0.0161 0.0008 0.0017 0.0008 0.001 0.0044 0.0001 0.0001 0.0067 0.0001 0.0003 0.0003
None 0.5696 0.4248 0.5097 0.5394 0.5368 0.3998 0.579 0.6964 0.7219 0.8078 0.8399 0.727 0.7493 0.649 0.5883 0.6572 0.6706 0.5851
Nuclear Periphery 0.0052 0.0009 0.0014 0.001 0.0031 0.0011 0.0055 0.0004 0.0011 0.0005 0.0013 0.0029 0.0002 0.0004 0.0011 0.0004 0.0006 0.0011
Nucleolus 0.0005 0.0004 0.0002 0.0004 0.0004 0.0001 0.001 0.0006 0.0011 0.0003 0.0007 0.0003 0 0.0001 0.0004 0.0001 0.0001 0.0001
Nucleus 0.0061 0.0019 0.0009 0.0006 0.001 0.0007 0.0029 0.0023 0.0034 0.0042 0.0008 0.0026 0.0004 0.0005 0.0011 0.0005 0.0009 0.0009
Peroxisomes 0.0091 0.0004 0.0013 0.0131 0.035 0.0004 0.0103 0.0003 0.0047 0.0002 0.0011 0.0006 0 0 0.0006 0.0001 0.0002 0.0006
Punctate Nuclear 0.0059 0.0025 0.0016 0.001 0.0015 0.0006 0.0085 0.0014 0.0031 0.0009 0.0015 0.0054 0.0002 0.0002 0.0013 0.0004 0.0009 0.0013
Vacuole 0.0014 0.002 0.0014 0.0019 0.0019 0.0007 0.0034 0.0012 0.0014 0.001 0.0011 0.0004 0.0002 0.0006 0.0019 0.0004 0.0006 0.0004
Vacuole Periphery 0.0004 0.0002 0.0004 0.0006 0.0015 0.0002 0.0016 0.0002 0.0003 0.0002 0.0003 0.0002 0 0 0.0004 0.0001 0.0001 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 9.4494 10.9535 10.8833 14.4788 9.6936 12.2463 14.7825 14.3798 13.7888 16.1424
Translational Efficiency 0.5423 0.4391 0.4523 0.3172 0.5739 0.5335 0.4022 0.4164 0.4713 0.4155

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Protein kinase involved in cell proliferation in response to nutrients; glucose-repressible; involved in signal transduction during cell proliferation in response to nutrients, specifically the establishment of stationary phase; identified as a regulator of IME2; phosphorylates Igo1p and Igo2p; substrate of Pho80p-Pho85p kinase
Localization
Cell Percentages cytoplasm (32%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Rim15

Rim15


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rim15-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available