Standard name
Human Ortholog
Description Ribosomal 60S subunit protein L22A; required for translation of long 5' UTR of IME1 mRNA and meiotic entry; homologous to mammalian ribosomal protein L22, no bacterial homolog; RPL22B has a paralog, RPL22A, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0.08 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0.09 – 0.05 0 0 0.07 0 0 0 0.15 0.08 0.05 0 0 0 0 0 0
Cytoplasm 0.97 0.99 0.98 0.97 0.95 0.87 0.8 – 0.73 0.72 0.64 0.57 0.98 0.97 0.97 0.93 0.96 0.88 0.92 0.95 0.96 0.95 0.92 0.9
Endoplasmic Reticulum 0 0 0 0 0 0 0.13 – 0.15 0.12 0.16 0.19 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 – 0.12 0.05 0.12 0.14 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0.07 0 – 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 2 2 – 5 5 3 12 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 2 0 0 0 0 1 – 0 0 1 0 1 0 0 0 0 0 0 1 0 0 2 3
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 2 0 1 6 0 15 – 7 6 3 10 7 4 9 12 9 4 0 0 0 0 0 0
Cytoplasm 202 155 57 62 134 66 133 – 103 99 64 87 225 353 401 76 110 76 185 150 57 202 315 265
Endoplasmic Reticulum 2 2 1 2 5 2 21 – 21 16 16 29 6 14 11 2 2 3 3 1 0 4 13 13
Endosome 1 0 0 0 0 0 1 – 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 2 – 3 5 3 7 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 1 0 – 17 7 12 22 0 0 0 1 1 1 0 0 0 0 2 1
Nucleus 0 0 0 0 0 0 1 – 1 2 2 2 0 0 0 0 1 4 2 1 0 0 1 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 1 0 0 0 1 0 2 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 1 2 0 0 0 0 0
Vac/Vac Membrane 4 0 0 0 0 5 3 – 0 6 5 6 0 0 2 2 1 2 0 0 1 1 4 6
Unique Cell Count 208 157 58 64 141 76 166 141 137 100 153 229 365 412 82 114 86 201 158 60 214 342 296
Labelled Cell Count 209 161 58 65 145 76 179 157 146 109 176 239 371 424 94 124 94 201 158 60 214 342 296


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 83.6 78.2 68.2 55.0 48.0 23.7 29.1 – 19.8 20.0 16.2 17.5 74.1 65.6 66.4 46.6 46.6 44.3 69.6 73.2 70.8
Std Deviation (1e-4) 26.3 22.3 16.3 13.8 11.9 7.4 8.5 – 5.4 5.1 4.5 4.7 17.4 17.0 17.4 25.5 21.2 23.1 19.0 21.7 23.3
Intensity Change (Log2) – – – -0.31 -0.51 -1.52 -1.23 – -1.78 -1.77 -2.08 -1.96 0.12 -0.06 -0.04 -0.55 -0.55 -0.62 0.03 0.1 0.06


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cytoplasm -0.5 -1.1 -2.4 -3.3 – -4.1 -4.2 -4.9 -5.8 0 -0.6 -0.4 -1.5 -0.7 -2.2
Endoplasmic Reticulum 0 0 0 2.4 – 2.7 2.3 2.8 3.2 0 0 0 0 0 0
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 – 0 0 0 3.1 0 0 0 0 0 0
Nucleus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 46.5396 63.7939 56.0017 52.7545 48.4634 56.2395 57.5735 88.9956 83.937 68.1307 65.7055 76.0255 63.5859 83.4368 73.3486 74.3268 73.8713 76.391
Actin 0.0013 0.0005 0.0017 0.0004 0.0005 0.0015 0.0108 0.0001 0.0065 0.0002 0.0013 0.0014 0.0025 0.0004 0.0029 0.0002 0.0005 0.0007
Bud 0.0014 0.0003 0.0008 0.0007 0.0007 0.0006 0.0018 0.0003 0.0024 0.0007 0.0052 0.0008 0.001 0.0004 0.0007 0.0009 0.0008 0.0005
Bud Neck 0.0005 0.0002 0.0006 0.0005 0.0012 0.0015 0.0008 0.0002 0.0004 0.0006 0.0006 0.0022 0.0006 0.0003 0.0003 0.0003 0.0003 0.0009
Bud Periphery 0.0022 0.0001 0.0003 0.0002 0.0004 0.0003 0.0017 0.0001 0.0017 0.0006 0.0082 0.0005 0.0012 0.0002 0.0004 0.0003 0.0003 0.0002
Bud Site 0.0006 0.0002 0.0003 0.0003 0.0008 0.0004 0.0025 0.0057 0.0005 0.0002 0.0027 0.0003 0.0011 0.0003 0.0002 0.0001 0.0001 0
Cell Periphery 0.0053 0.0015 0.0007 0.0003 0.0037 0.0009 0.0055 0.0012 0.0016 0.0028 0.0027 0.0008 0.005 0.0004 0.0009 0.0019 0.0003 0.0003
Cytoplasm 0.7767 0.9706 0.9082 0.8528 0.7553 0.8672 0.7131 0.9725 0.939 0.8326 0.8405 0.8943 0.8359 0.977 0.9194 0.8627 0.8994 0.903
Cytoplasmic Foci 0.0046 0.0017 0.0044 0.0032 0.0118 0.0091 0.026 0.0006 0.0034 0.0127 0.0247 0.0043 0.0098 0.0012 0.0031 0.0024 0.0034 0.0044
Eisosomes 0.0002 0 0.0001 0 0.0007 0 0.0002 0 0 0.0001 0.0001 0.0001 0.0002 0 0 0 0 0
Endoplasmic Reticulum 0.057 0.0043 0.0108 0.0121 0.065 0.0166 0.0316 0.0027 0.0024 0.002 0.0048 0.0258 0.0516 0.0037 0.0147 0.0032 0.005 0.0294
Endosome 0.0186 0.0029 0.0115 0.0327 0.0106 0.0307 0.0202 0.0006 0.0019 0.0061 0.012 0.0078 0.0105 0.0023 0.0069 0.0098 0.0155 0.0164
Golgi 0.0036 0.0005 0.0017 0.0052 0.0015 0.0047 0.0057 0.0001 0.0008 0.0006 0.0014 0.0011 0.0018 0.0005 0.003 0.0002 0.0005 0.0015
Lipid Particles 0.001 0.0007 0.002 0.0088 0.0056 0.0132 0.0087 0.0002 0.0005 0.0058 0.0058 0.0027 0.0027 0.0001 0.0004 0.0002 0.0001 0
Mitochondria 0.0111 0.0002 0.0019 0.003 0.018 0.0056 0.0196 0 0.0049 0.0095 0.016 0.0232 0.004 0.0011 0.0029 0.0002 0.0028 0.0021
None 0.0022 0.0002 0.0009 0.0003 0.0082 0.001 0.0064 0.0001 0.0013 0.0027 0.0021 0.0013 0.0078 0.0001 0.0006 0.0002 0.0006 0.0003
Nuclear Periphery 0.0208 0.0014 0.009 0.008 0.0033 0.0069 0.0291 0.0017 0.0019 0.0046 0.0081 0.0044 0.0203 0.0015 0.0072 0.0053 0.0076 0.0102
Nucleolus 0.0013 0 0.0001 0.0004 0.0561 0.0006 0.003 0 0.0007 0.0264 0.0016 0.0006 0.0017 0 0.0015 0.0002 0.0001 0
Nucleus 0.0089 0.0009 0.0017 0.0022 0.0097 0.0025 0.0238 0.0008 0.0019 0.0087 0.005 0.0025 0.0049 0.0012 0.0037 0.0033 0.0035 0.0017
Peroxisomes 0.0001 0 0.0024 0.0005 0.0008 0.002 0.0021 0 0.0002 0.0007 0.0006 0.0004 0.0003 0 0.0001 0 0 0
Punctate Nuclear 0.0032 0 0.0002 0.0003 0.0011 0.0005 0.01 0 0.0001 0.0015 0.0044 0.0001 0.0028 0 0.0001 0 0.0001 0
Vacuole 0.0609 0.012 0.0307 0.0516 0.0277 0.0249 0.0623 0.0123 0.0241 0.0651 0.0426 0.0154 0.0266 0.008 0.0245 0.0889 0.0442 0.017
Vacuole Periphery 0.0188 0.0019 0.01 0.0164 0.0176 0.0092 0.0151 0.0006 0.0035 0.0157 0.0098 0.0099 0.0076 0.0012 0.0067 0.0196 0.0148 0.0113

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 98.6784 213.8485 243.9779 177.1734 104.6789 104.601 187.1672 181.507 176.6355 145.7818
Translational Efficiency 1.7429 1.9902 2.055 2.3986 2.6107 1.9336 2.4751 2.5641 1.8306 2.2047

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2007 689 1664 1412 1330 1865 288 1149 3337 2554 1952 2561

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 4496.53 5655.06 7266.65 2890.26 4614.96 5041.15 3830.13 2537.44 4543.73 5206.77 6759.62 2731.97
Standard Deviation 1092.42 1617.83 1570.45 1316.12 1088.83 1399.24 1178.51 1010.43 1092.53 1486.62 1947.48 1201.61
Intensity Change Log 2 — 0.330730 0.692478 -0.637613 — 0.127435 -0.268925 -0.862944 — 0.231341 0.284382 -0.747346

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000040 0.000119 0.000017 0.000292 0.000069 0.000069 0.000386 0.000421 0.000051 0.000082 0.000072 0.000350
Bud Neck 0.000135 0.000986 0.000214 0.002168 0.000426 0.000369 0.005363 0.002131 0.000251 0.000535 0.000973 0.002151
Bud Site 0.002264 0.005333 0.001914 0.017899 0.001985 0.005189 0.014664 0.022704 0.002153 0.005228 0.003795 0.020055
Cell Periphery 0.007202 0.029160 0.001757 0.005705 0.014982 0.006435 0.016251 0.013782 0.010303 0.012566 0.003895 0.009329
Cytoplasm 0.757496* 0.647276* 0.797466* 0.676085* 0.676910* 0.827318* 0.600677* 0.792652* 0.725377* 0.778748* 0.768432* 0.728383*
Cytoplasmic Foci 0.018500 0.014913 0.008249 0.015579 0.009608 0.012075 0.027752 0.016655 0.014956 0.012841 0.011126 0.016062
Eisosomes 0.000001 0.000003 0.000000 0.000003 0.000001 0.000001 0.000005 0.000004 0.000001 0.000001 0.000001 0.000003
Endoplasmic Reticulum 0.001922 0.000789 0.000731 0.000534 0.001109 0.000631 0.000324 0.000361 0.001598 0.000674 0.000671 0.000456
Endosome 0.000848 0.001043 0.000623 0.004360 0.000815 0.000922 0.002890 0.002101 0.000835 0.000954 0.000958 0.003347
Golgi 0.068568 0.082180 0.014254 0.023848 0.084338 0.037389 0.055487 0.017411 0.074853 0.049472 0.020337 0.020960
Lipid Particles 0.008502 0.021923 0.001726 0.009659 0.012439 0.004756 0.021836 0.013844 0.010071 0.009387 0.004693 0.011536
Mitochondria 0.000262 0.000128 0.000398 0.000812 0.000106 0.000288 0.000116 0.000188 0.000200 0.000245 0.000356 0.000532
Mitotic Spindle 0.001051 0.001573 0.000927 0.012123 0.001200 0.001915 0.006008 0.003008 0.001110 0.001823 0.001677 0.008034
None 0.001692 0.002934 0.000898 0.000844 0.002176 0.002623 0.002066 0.001767 0.001885 0.002707 0.001070 0.001258
Nuclear Periphery 0.000323 0.000846 0.000235 0.000348 0.000934 0.000275 0.000924 0.000201 0.000566 0.000429 0.000337 0.000282
Nuclear Periphery Foci 0.010291 0.016910 0.004140 0.006440 0.020305 0.006687 0.016674 0.005332 0.014282 0.009445 0.005989 0.005943
Nucleolus 0.000038 0.000103 0.000068 0.000224 0.000053 0.000066 0.000126 0.000143 0.000044 0.000076 0.000077 0.000187
Nucleus 0.002685 0.011212 0.004582 0.013291 0.005894 0.006379 0.025554 0.016049 0.003964 0.007683 0.007676 0.014528
Peroxisomes 0.000023 0.000031 0.000027 0.000167 0.000013 0.000032 0.000060 0.000193 0.000019 0.000032 0.000032 0.000178
Vacuole 0.035113 0.094720 0.089656 0.167746 0.071120 0.041891 0.172512 0.079480 0.049464 0.056143 0.101880 0.128145
Vacuole Periphery 0.083044 0.067818 0.072118 0.041873 0.095515 0.044690 0.030325 0.011575 0.088015 0.050930 0.065952 0.028280

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -5.61 3.29 -4.66 -3.10 -5.09 0.02 -5.68 -5.30 -5.37 -0.22 -2.82 -2.23 -7.10 -6.35 -6.41
Bud Neck -6.66 -1.37 -14.11 -8.22 -13.93 0.75 -7.86 -4.71 -4.82 3.80 -4.95 -6.76 -9.90 -8.79 -5.82
Bud Site -4.50 1.62 -11.67 -8.71 -11.93 -5.90 -6.59 -10.78 -9.08 -2.62 -7.62 -4.67 -15.80 -12.75 -13.80
Cell Periphery -10.31 10.09 3.13 11.34 -5.98 7.97 -0.24 1.76 -4.46 1.69 -2.66 11.53 2.60 4.62 -6.59
Cytoplasm 7.69 -3.62 8.32 -1.50 11.61 -13.69 4.05 -8.77 4.71 -9.39 -6.43 -4.39 0.84 7.08 5.06
Cytoplasmic Foci 1.68 7.49 1.27 -0.54 -6.42 -2.66 -7.67 -4.99 -3.53 3.90 1.85 3.10 -2.00 -3.72 -4.78
Eisosomes -5.19 5.14 -8.30 -0.21 -9.83 2.73 -5.46 -5.53 -6.36 1.52 -3.39 -1.48 -9.20 -7.03 -7.86
Endoplasmic Reticulum 4.53 5.13 6.31 2.43 2.82 3.09 5.53 5.15 3.43 -0.73 5.91 5.94 7.86 3.72 3.42
Endosome -1.75 3.72 -6.83 -6.19 -7.29 -1.43 -5.33 -4.21 -3.71 1.83 -2.06 -1.78 -7.98 -7.35 -7.34
Golgi -2.70 19.56 16.21 14.01 -3.93 13.36 6.31 20.81 9.35 7.67 9.50 24.75 25.52 15.16 1.10
Lipid Particles -6.49 8.95 -0.53 5.81 -6.86 6.92 -3.91 -0.10 -6.15 3.68 0.87 7.55 -0.65 -1.39 -6.79
Mitochondria 3.62 -2.73 -1.95 -2.51 -1.39 -4.03 -1.53 -3.75 1.43 -2.62 -1.35 -4.20 -2.25 -1.82 -1.07
Mitotic Spindle -2.13 0.30 -6.98 -6.67 -6.94 -0.55 -2.98 -2.30 -2.26 1.57 -1.79 -1.26 -7.35 -6.89 -6.69
None -2.84 7.96 8.43 4.66 0.04 -0.62 0.33 1.23 1.12 0.46 -1.54 6.84 3.92 2.60 -1.47
Nuclear Periphery -7.54 1.46 -1.83 6.02 -2.81 7.70 -0.59 8.09 0.76 7.66 2.58 4.51 5.62 2.33 1.39
Nuclear Periphery Foci -5.02 7.88 5.79 8.59 -2.43 11.76 2.22 13.05 1.95 7.36 6.48 11.34 13.34 6.50 0.91
Nucleolus -5.71 -7.39 -12.60 -7.13 -10.57 -2.14 -7.36 -4.65 -4.10 -1.69 -5.48 -9.56 -10.64 -8.24 -8.33
Nucleus -8.41 -4.05 -13.05 -4.79 -9.78 -1.30 -8.63 -9.89 -9.01 2.35 -7.17 -6.87 -16.30 -11.09 -9.41
Peroxisomes -1.21 -0.74 -7.32 -6.57 -7.06 -4.65 -4.75 -5.66 -5.06 -3.63 -3.78 -3.36 -8.86 -8.08 -7.95
Vacuole -10.12 -13.02 -22.31 -9.49 -11.19 6.76 -8.04 -2.24 -8.19 6.93 -2.77 -12.81 -19.50 -16.68 -5.01
Vacuole Periphery 2.93 2.60 12.00 6.47 9.49 11.10 13.63 20.57 14.22 5.57 11.68 6.32 22.33 10.86 13.86
Standard name
Human Ortholog
Description Ribosomal 60S subunit protein L22A; required for translation of long 5' UTR of IME1 mRNA and meiotic entry; homologous to mammalian ribosomal protein L22, no bacterial homolog; RPL22B has a paralog, RPL22A, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-2

Rpl22b

Rpl22b


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rpl22b-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available