Standard name
Human Ortholog
Description Mitochondrial RNA polymerase; single subunit enzyme similar to those of T3 and T7 bacteriophages; requires a specificity subunit encoded by MTF1 for promoter recognition; Mtf1p interacts with and stabilizes the Rpo41p-promoter complex, enhancing DNA bending and melting to facilitate pre-initiation open complex formation; Rpo41p also synthesizes RNA primers for mitochondrial DNA replication

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.99 0.96 0.99 0.99 0.98 0.97 0.99 0.99 0.97 0.96 0.83 0.94 0.89 0.89
Endoplasmic Reticulum 0 0 0 0 0 0.05 0.05 0 0 0 0 0 0.06 0.07
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 1 0 0 0 0 0 0 2 4 0 1 1 2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0
Cell Periphery 5 5 1 6 7 15 9 11 0 0 0 0 0 0
Cytoplasm 346 587 199 237 242 540 342 436 337 598 16 162 208 284
Endoplasmic Reticulum 3 23 3 2 4 29 16 18 3 4 0 5 15 21
Endosome 0 0 0 0 0 0 0 1 0 0 0 0 0 0
Golgi 0 1 0 0 0 5 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 1 0 1 0 0 0 0 0 2
Nucleus 0 0 0 2 1 0 0 0 0 2 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 1 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 1 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 2 1 0 2 1 7 3 2 0 10 0 1 2 4
Unique Cell Count 350 609 201 240 248 556 346 442 346 625 20 173 233 321
Labelled Cell Count 356 618 203 249 257 597 370 469 346 625 20 173 233 321


Punctate Nuclear

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 56.9 56.6 51.3 46.3 39.7 63.5 68.5 67.7 52.2 57.0 55.4
Std Deviation (1e-4) 13.3 11.8 11.4 10.4 8.9 20.9 16.7 19.0 13.0 15.4 14.5
Intensity Change (Log2)


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud
Bud Neck
Bud Site
Cell Periphery
Cyto
Endoplasmic Reticulum
Endosome
Golgi
Mitochondria
Nuclear Periphery
Nuc
Nucleolus
Peroxisomes
SpindlePole
Vac
Cortical Patches
Cytoplasm
Nucleus
Vacuole

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 36.4896 50.3495 45.0214 43.2528 38.0049 45.7951 40.8934 57.6413 51.9737 50.7842 45.9184 53.2937 50.5676 62.156 57.1701 52.1128 51.7062 56.3198
Actin 0.0012 0.0004 0.0005 0.0001 0.0013 0.0016 0.0064 0.0012 0.002 0.0207 0.0012 0.0008 0.002 0.0002 0.0007 0.0002 0.0019 0.0011
Bud 0.0008 0.0007 0.0018 0.001 0.0015 0.0013 0.0015 0.0002 0.0011 0.0009 0.0011 0.0003 0.0009 0.0003 0.0006 0.0009 0.0006 0.0005
Bud Neck 0.0008 0.0013 0.0014 0.0009 0.003 0.0055 0.0015 0.0002 0.0009 0.0019 0.0006 0.0022 0.0004 0.0004 0.0006 0.0011 0.0006 0.0016
Bud Periphery 0.0014 0.0005 0.0017 0.0006 0.0007 0.0022 0.002 0.0001 0.0012 0.0024 0.0029 0.0002 0.0014 0.0003 0.0005 0.0004 0.0006 0.0005
Bud Site 0.0005 0.0011 0.0009 0.0002 0.0006 0.0003 0.0055 0.0047 0.002 0.0066 0.0006 0.0001 0.0004 0.0003 0.0003 0.0002 0.0012 0.0001
Cell Periphery 0.0127 0.0093 0.0093 0.0039 0.0027 0.0057 0.0039 0.0018 0.0014 0.0066 0.0024 0.0012 0.0027 0.0079 0.003 0.001 0.0014 0.0014
Cytoplasm 0.6542 0.8624 0.7698 0.7515 0.7115 0.746 0.7614 0.9537 0.8822 0.8638 0.7713 0.9051 0.8687 0.9344 0.9218 0.8229 0.8825 0.9017
Cytoplasmic Foci 0.0021 0.001 0.0019 0.0013 0.0054 0.0037 0.0111 0.0019 0.0077 0.0023 0.0058 0.0038 0.0054 0.0011 0.0032 0.0081 0.0045 0.006
Eisosomes 0 0 0 0 0 0 0.0002 0.0001 0.0001 0.003 0.0001 0 0.0001 0 0 0 0 0
Endoplasmic Reticulum 0.0979 0.0375 0.021 0.0083 0.0514 0.1194 0.0454 0.0154 0.0187 0.0233 0.032 0.0531 0.0408 0.0268 0.0229 0.0089 0.0094 0.0359
Endosome 0.0076 0.0029 0.0068 0.0097 0.0198 0.0235 0.0199 0.0016 0.0133 0.007 0.0476 0.011 0.0125 0.0019 0.0065 0.019 0.0284 0.0184
Golgi 0.0007 0.0003 0.0005 0.0001 0.0011 0.0031 0.0036 0.0003 0.0024 0.0017 0.0085 0.0023 0.0032 0.0003 0.0014 0.0009 0.006 0.0048
Lipid Particles 0.0026 0.0006 0.0011 0.0002 0.0011 0.0003 0.0073 0.0029 0.0053 0.0017 0.0045 0.0008 0.0039 0.0006 0.001 0.0008 0.0012 0.0016
Mitochondria 0.0021 0.0006 0.0011 0.0001 0.0006 0.0041 0.0064 0.0016 0.0138 0.0084 0.0236 0.0009 0.0032 0.0001 0.0027 0.0028 0.0038 0.0084
None 0.0005 0.0002 0.0002 0.0002 0.0003 0.0004 0.007 0.0005 0.0036 0.0004 0.0026 0.0002 0.0013 0.0001 0.0002 0.0033 0.0003 0.0003
Nuclear Periphery 0.0366 0.007 0.0136 0.0077 0.0105 0.0137 0.0171 0.0013 0.0072 0.0033 0.0061 0.0048 0.0051 0.0012 0.0018 0.0031 0.0021 0.0021
Nucleolus 0.0002 0.0002 0.0002 0.0001 0.0001 0.0001 0.0032 0.0003 0.0007 0.0001 0.0003 0.0001 0.0006 0 0.0001 0.0009 0.0001 0
Nucleus 0.0089 0.003 0.0045 0.0039 0.0049 0.0037 0.0201 0.0009 0.0023 0.0015 0.0022 0.0016 0.0034 0.0007 0.0017 0.0027 0.0014 0.001
Peroxisomes 0.0073 0 0 0 0.0001 0.0001 0.0019 0.0003 0.0017 0.0002 0.0002 0 0.0014 0 0.003 0.0003 0.0002 0.0002
Punctate Nuclear 0.0002 0 0.0001 0 0.0003 0.0001 0.0058 0.0003 0.002 0.0001 0.0002 0.0001 0.0008 0 0 0.0001 0.0001 0.0002
Vacuole 0.1403 0.0642 0.1446 0.1833 0.1485 0.0542 0.0602 0.0101 0.0253 0.0405 0.0713 0.0087 0.0359 0.0221 0.0241 0.1025 0.0467 0.0113
Vacuole Periphery 0.0215 0.0066 0.0189 0.0268 0.0345 0.011 0.0087 0.0005 0.0049 0.0037 0.0149 0.0024 0.0058 0.0013 0.0038 0.0199 0.0071 0.0029

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 19.315 22.8598 26.1663 30.1475 18.5175 24.4703 27.478 36.2619 34.3916 27.7114
Translational Efficiency 0.5808 0.4792 0.6171 0.5059 0.6037 0.6282 0.6031 0.4925 0.5649 0.5889

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
157 678 153 1178 1434 2045 470 1941 1591 2723 623 3119

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 3684.04 3450.44 5307.17 4100.91 3370.36 3570.24 4709.36 4640.57 3401.31 3540.41 4856.17 4436.75
Standard Deviation 714.32 736.69 848.19 995.00 704.16 771.65 742.93 954.44 711.35 764.85 811.97 1004.63
Intensity Change Log 2 -0.094508 0.526654 0.154655 0.083118 0.482628 0.461399 -0.006913 0.505788 0.309354

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000100 0.000103 0.000290 0.000205 0.000037 0.000119 0.000149 0.000324 0.000043 0.000115 0.000184 0.000279
Bud Neck 0.000519 0.000713 0.008814 0.001756 0.000145 0.000453 0.005714 0.000961 0.000181 0.000518 0.006476 0.001261
Bud Site 0.004753 0.003417 0.003491 0.012137 0.002029 0.003553 0.007226 0.009949 0.002298 0.003519 0.006309 0.010775
Cell Periphery 0.033479 0.008634 0.061753 0.010808 0.005009 0.002456 0.006691 0.028831 0.007819 0.003994 0.020213 0.022024
Cytoplasm 0.671902* 0.838225* 0.313053* 0.656946* 0.847918* 0.890436* 0.509585* 0.739018* 0.830549* 0.877436* 0.461320* 0.708020*
Cytoplasmic Foci 0.013595 0.006845 0.027351 0.005921 0.010820 0.008406 0.012948 0.002755 0.011094 0.008018 0.016485 0.003951
Eisosomes 0.000002 0.000001 0.000015 0.000003 0.000001 0.000001 0.000003 0.000004 0.000001 0.000001 0.000006 0.000003
Endoplasmic Reticulum 0.000652 0.000781 0.000259 0.000278 0.001326 0.000451 0.000331 0.000365 0.001259 0.000533 0.000313 0.000332
Endosome 0.001050 0.000744 0.001252 0.002188 0.000633 0.000761 0.001663 0.000592 0.000674 0.000757 0.001562 0.001195
Golgi 0.093357 0.038786 0.061351 0.013205 0.041120 0.028260 0.032641 0.008251 0.046275 0.030881 0.039692 0.010122
Lipid Particles 0.018994 0.011563 0.044953 0.012141 0.005928 0.003510 0.013281 0.020926 0.007218 0.005515 0.021059 0.017608
Mitochondria 0.000084 0.000145 0.000155 0.000561 0.000272 0.000138 0.000105 0.001139 0.000253 0.000140 0.000118 0.000921
Mitotic Spindle 0.001429 0.001009 0.002889 0.002508 0.000586 0.001182 0.004220 0.000505 0.000669 0.001139 0.003893 0.001262
None 0.001685 0.002076 0.001563 0.001528 0.001224 0.001283 0.000850 0.000795 0.001269 0.001481 0.001025 0.001072
Nuclear Periphery 0.000368 0.000266 0.005409 0.000258 0.000159 0.000208 0.001435 0.000189 0.000180 0.000222 0.002411 0.000215
Nuclear Periphery Foci 0.012568 0.010987 0.030926 0.003986 0.007329 0.006592 0.020633 0.003596 0.007846 0.007687 0.023161 0.003743
Nucleolus 0.000050 0.000107 0.000153 0.000301 0.000030 0.000057 0.000186 0.000204 0.000032 0.000069 0.000178 0.000241
Nucleus 0.005966 0.012056 0.038067 0.012544 0.001923 0.005446 0.021945 0.005846 0.002322 0.007092 0.025904 0.008376
Peroxisomes 0.000022 0.000021 0.000017 0.000138 0.000031 0.000025 0.000026 0.000126 0.000030 0.000024 0.000024 0.000131
Vacuole 0.060285 0.037089 0.340657* 0.236225* 0.026915 0.026045 0.311219* 0.163195 0.030208 0.028794 0.318448* 0.190778
Vacuole Periphery 0.079141 0.026430 0.057583 0.026362 0.046566 0.020616 0.049150 0.012431 0.049780 0.022063 0.051221 0.017692

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -0.31 -4.51 -2.60 -2.76 2.49 -2.20 -6.92 -2.11 -1.38 -0.87 -2.51 -8.96 -2.71 -1.66 -0.51
Bud Neck -1.37 -5.18 -8.49 -6.98 4.13 -4.88 -10.08 -9.71 -5.04 8.89 -5.77 -11.18 -13.82 -9.05 9.25
Bud Site 0.80 0.65 -5.16 -5.72 -5.91 -3.07 -4.57 -7.61 -5.43 -0.67 -2.99 -4.68 -10.42 -7.83 -2.42
Cell Periphery 5.04 -3.61 5.00 -0.32 9.36 5.05 -2.35 -15.68 -18.10 -14.02 5.16 -6.69 -11.41 -16.97 -0.72
Cytoplasm -5.72 10.27 1.14 14.76 -13.11 -4.98 21.69 12.62 19.16 -14.54 -5.67 26.15 15.70 25.57 -17.64
Cytoplasmic Foci 1.90 -2.94 1.98 0.33 5.05 2.09 -3.30 8.31 9.26 8.72 2.94 -4.84 7.24 7.29 9.35
Eisosomes 1.40 -6.72 -1.28 -5.49 6.53 -1.62 -8.80 -4.04 -3.76 0.79 -1.15 -10.42 -5.27 -4.98 4.92
Endoplasmic Reticulum -0.71 4.08 4.13 3.28 0.35 5.01 5.87 5.61 1.42 -0.66 4.52 6.22 6.16 3.52 -0.27
Endosome 1.75 -1.43 -2.38 -3.29 -1.74 -1.77 -7.03 0.34 1.68 6.66 -1.43 -7.67 -2.93 -2.14 2.83
Golgi 5.15 3.27 8.11 8.27 11.30 4.40 4.06 13.41 11.85 11.69 5.42 3.43 15.02 14.12 15.22
Lipid Particles 2.17 -4.66 2.30 -0.10 6.65 3.22 -6.56 -10.62 -13.49 -3.81 1.95 -8.70 -9.54 -12.30 2.59
Mitochondria -1.04 -3.45 -3.39 -2.64 -2.68 2.87 3.24 -1.53 -1.76 -1.78 2.59 2.89 -1.85 -2.16 -2.17
Mitotic Spindle 1.13 -2.66 -1.30 -1.77 0.59 -3.17 -3.52 0.35 3.00 3.54 -3.05 -4.02 -1.87 -0.21 3.24
None -1.22 0.59 0.71 1.48 0.41 -0.32 2.07 1.93 2.45 0.38 -1.21 1.51 1.11 2.18 0.11
Nuclear Periphery 1.87 -9.14 1.60 -0.05 9.31 -1.60 -8.30 -2.08 0.49 8.09 -1.74 -11.84 -2.20 0.09 11.60
Nuclear Periphery Foci 0.67 -5.73 4.36 6.95 10.64 0.72 -6.50 5.26 5.25 8.64 0.03 -8.93 6.34 8.04 12.01
Nucleolus -1.29 -8.61 -4.89 -2.92 -2.71 -1.66 -14.08 -2.69 -2.15 0.15 -2.27 -16.33 -4.71 -3.57 -0.92
Nucleus -2.50 -6.88 -5.73 -2.07 4.88 -8.33 -10.85 -8.16 -2.09 8.68 -8.07 -13.17 -12.56 -4.26 9.28
Peroxisomes 0.03 0.52 -4.15 -4.18 -4.31 0.49 -0.28 -7.49 -7.24 -7.46 0.69 0.22 -7.55 -7.63 -7.75
Vacuole 2.45 -12.87 -16.30 -25.29 4.39 -0.07 -22.66 -27.30 -27.50 11.13 0.20 -26.81 -36.28 -37.47 10.95
Vacuole Periphery 3.92 1.82 4.26 1.23 5.34 8.62 1.30 12.60 5.60 8.45 9.22 1.48 11.94 4.23 9.42
Standard name
Human Ortholog
Description Mitochondrial RNA polymerase; single subunit enzyme similar to those of T3 and T7 bacteriophages; requires a specificity subunit encoded by MTF1 for promoter recognition; Mtf1p interacts with and stabilizes the Rpo41p-promoter complex, enhancing DNA bending and melting to facilitate pre-initiation open complex formation; Rpo41p also synthesizes RNA primers for mitochondrial DNA replication
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-2

Rpo41

Rpo41


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rpo41-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available