| Standard name | |
|---|---|
| Human Ortholog | |
| Description | Mitochondrial RNA polymerase; single subunit enzyme similar to those of T3 and T7 bacteriophages; requires a specificity subunit encoded by MTF1 for promoter recognition; Mtf1p interacts with and stabilizes the Rpo41p-promoter complex, enhancing DNA bending and melting to facilitate pre-initiation open complex formation; Rpo41p also synthesizes RNA primers for mitochondrial DNA replication |
Micrographs
Sub-cellular Localization
Yeast GFP Assignment
Protein Abundance
Localization Change
External localization resources
| ensLOC | DeepLoc | |||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
| Cortical Patches | 0 | 0 | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| Bud | 0 | 1 | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 4 | 0 | 1 | 1 | 2 |
| Bud Neck | 0 | 0 | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| Bud Site | 0 | 0 | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
| Cell Periphery | 5 | 5 | – | – | – | – | – | – | – | – | – | – | 1 | 6 | 7 | 15 | 9 | 11 | 0 | 0 | 0 | 0 | 0 | 0 |
| Cytoplasm | 346 | 587 | – | – | – | – | – | – | – | – | – | – | 199 | 237 | 242 | 540 | 342 | 436 | 337 | 598 | 16 | 162 | 208 | 284 |
| Endoplasmic Reticulum | 3 | 23 | – | – | – | – | – | – | – | – | – | – | 3 | 2 | 4 | 29 | 16 | 18 | 3 | 4 | 0 | 5 | 15 | 21 |
| Endosome | 0 | 0 | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
| Golgi | 0 | 1 | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 | 5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| Mitochondria | 0 | 0 | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 2 |
| Nucleus | 0 | 0 | – | – | – | – | – | – | – | – | – | – | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 |
| Nuclear Periphery | 0 | 0 | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| Nucleolus | 0 | 0 | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| Peroxisomes | 0 | 0 | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| SpindlePole | 0 | 0 | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| Vac/Vac Membrane | 2 | 1 | – | – | – | – | – | – | – | – | – | – | 0 | 2 | 1 | 7 | 3 | 2 | 0 | 10 | 0 | 1 | 2 | 4 |
| Unique Cell Count | 350 | 609 | 201 | 240 | 248 | 556 | 346 | 442 | 346 | 625 | 20 | 173 | 233 | 321 | ||||||||||
| Labelled Cell Count | 356 | 618 | 203 | 249 | 257 | 597 | 370 | 469 | 346 | 625 | 20 | 173 | 233 | 321 | ||||||||||
Yeast GFP Assignment
Protein Abundance
| Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Mean Cell GFP Intensity (1e-4) | 56.9 | 56.6 | – | – | – | – | – | – | – | – | – | – | 51.3 | 46.3 | 39.7 | 63.5 | 68.5 | 67.7 | 52.2 | 57.0 | 55.4 |
| Std Deviation (1e-4) | 13.3 | 11.8 | – | – | – | – | – | – | – | – | – | – | 11.4 | 10.4 | 8.9 | 20.9 | 16.7 | 19.0 | 13.0 | 15.4 | 14.5 |
| Intensity Change (Log2) | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Localization Change
| Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
External localization resources
Images
Protein Concentration and Protein Localization Data
| R1 | R2 | R3 | ||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
| Concentration | 36.4896 | 50.3495 | 45.0214 | 43.2528 | 38.0049 | 45.7951 | 40.8934 | 57.6413 | 51.9737 | 50.7842 | 45.9184 | 53.2937 | 50.5676 | 62.156 | 57.1701 | 52.1128 | 51.7062 | 56.3198 |
| Actin | 0.0012 | 0.0004 | 0.0005 | 0.0001 | 0.0013 | 0.0016 | 0.0064 | 0.0012 | 0.002 | 0.0207 | 0.0012 | 0.0008 | 0.002 | 0.0002 | 0.0007 | 0.0002 | 0.0019 | 0.0011 |
| Bud | 0.0008 | 0.0007 | 0.0018 | 0.001 | 0.0015 | 0.0013 | 0.0015 | 0.0002 | 0.0011 | 0.0009 | 0.0011 | 0.0003 | 0.0009 | 0.0003 | 0.0006 | 0.0009 | 0.0006 | 0.0005 |
| Bud Neck | 0.0008 | 0.0013 | 0.0014 | 0.0009 | 0.003 | 0.0055 | 0.0015 | 0.0002 | 0.0009 | 0.0019 | 0.0006 | 0.0022 | 0.0004 | 0.0004 | 0.0006 | 0.0011 | 0.0006 | 0.0016 |
| Bud Periphery | 0.0014 | 0.0005 | 0.0017 | 0.0006 | 0.0007 | 0.0022 | 0.002 | 0.0001 | 0.0012 | 0.0024 | 0.0029 | 0.0002 | 0.0014 | 0.0003 | 0.0005 | 0.0004 | 0.0006 | 0.0005 |
| Bud Site | 0.0005 | 0.0011 | 0.0009 | 0.0002 | 0.0006 | 0.0003 | 0.0055 | 0.0047 | 0.002 | 0.0066 | 0.0006 | 0.0001 | 0.0004 | 0.0003 | 0.0003 | 0.0002 | 0.0012 | 0.0001 |
| Cell Periphery | 0.0127 | 0.0093 | 0.0093 | 0.0039 | 0.0027 | 0.0057 | 0.0039 | 0.0018 | 0.0014 | 0.0066 | 0.0024 | 0.0012 | 0.0027 | 0.0079 | 0.003 | 0.001 | 0.0014 | 0.0014 |
| Cytoplasm | 0.6542 | 0.8624 | 0.7698 | 0.7515 | 0.7115 | 0.746 | 0.7614 | 0.9537 | 0.8822 | 0.8638 | 0.7713 | 0.9051 | 0.8687 | 0.9344 | 0.9218 | 0.8229 | 0.8825 | 0.9017 |
| Cytoplasmic Foci | 0.0021 | 0.001 | 0.0019 | 0.0013 | 0.0054 | 0.0037 | 0.0111 | 0.0019 | 0.0077 | 0.0023 | 0.0058 | 0.0038 | 0.0054 | 0.0011 | 0.0032 | 0.0081 | 0.0045 | 0.006 |
| Eisosomes | 0 | 0 | 0 | 0 | 0 | 0 | 0.0002 | 0.0001 | 0.0001 | 0.003 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 |
| Endoplasmic Reticulum | 0.0979 | 0.0375 | 0.021 | 0.0083 | 0.0514 | 0.1194 | 0.0454 | 0.0154 | 0.0187 | 0.0233 | 0.032 | 0.0531 | 0.0408 | 0.0268 | 0.0229 | 0.0089 | 0.0094 | 0.0359 |
| Endosome | 0.0076 | 0.0029 | 0.0068 | 0.0097 | 0.0198 | 0.0235 | 0.0199 | 0.0016 | 0.0133 | 0.007 | 0.0476 | 0.011 | 0.0125 | 0.0019 | 0.0065 | 0.019 | 0.0284 | 0.0184 |
| Golgi | 0.0007 | 0.0003 | 0.0005 | 0.0001 | 0.0011 | 0.0031 | 0.0036 | 0.0003 | 0.0024 | 0.0017 | 0.0085 | 0.0023 | 0.0032 | 0.0003 | 0.0014 | 0.0009 | 0.006 | 0.0048 |
| Lipid Particles | 0.0026 | 0.0006 | 0.0011 | 0.0002 | 0.0011 | 0.0003 | 0.0073 | 0.0029 | 0.0053 | 0.0017 | 0.0045 | 0.0008 | 0.0039 | 0.0006 | 0.001 | 0.0008 | 0.0012 | 0.0016 |
| Mitochondria | 0.0021 | 0.0006 | 0.0011 | 0.0001 | 0.0006 | 0.0041 | 0.0064 | 0.0016 | 0.0138 | 0.0084 | 0.0236 | 0.0009 | 0.0032 | 0.0001 | 0.0027 | 0.0028 | 0.0038 | 0.0084 |
| None | 0.0005 | 0.0002 | 0.0002 | 0.0002 | 0.0003 | 0.0004 | 0.007 | 0.0005 | 0.0036 | 0.0004 | 0.0026 | 0.0002 | 0.0013 | 0.0001 | 0.0002 | 0.0033 | 0.0003 | 0.0003 |
| Nuclear Periphery | 0.0366 | 0.007 | 0.0136 | 0.0077 | 0.0105 | 0.0137 | 0.0171 | 0.0013 | 0.0072 | 0.0033 | 0.0061 | 0.0048 | 0.0051 | 0.0012 | 0.0018 | 0.0031 | 0.0021 | 0.0021 |
| Nucleolus | 0.0002 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0032 | 0.0003 | 0.0007 | 0.0001 | 0.0003 | 0.0001 | 0.0006 | 0 | 0.0001 | 0.0009 | 0.0001 | 0 |
| Nucleus | 0.0089 | 0.003 | 0.0045 | 0.0039 | 0.0049 | 0.0037 | 0.0201 | 0.0009 | 0.0023 | 0.0015 | 0.0022 | 0.0016 | 0.0034 | 0.0007 | 0.0017 | 0.0027 | 0.0014 | 0.001 |
| Peroxisomes | 0.0073 | 0 | 0 | 0 | 0.0001 | 0.0001 | 0.0019 | 0.0003 | 0.0017 | 0.0002 | 0.0002 | 0 | 0.0014 | 0 | 0.003 | 0.0003 | 0.0002 | 0.0002 |
| Punctate Nuclear | 0.0002 | 0 | 0.0001 | 0 | 0.0003 | 0.0001 | 0.0058 | 0.0003 | 0.002 | 0.0001 | 0.0002 | 0.0001 | 0.0008 | 0 | 0 | 0.0001 | 0.0001 | 0.0002 |
| Vacuole | 0.1403 | 0.0642 | 0.1446 | 0.1833 | 0.1485 | 0.0542 | 0.0602 | 0.0101 | 0.0253 | 0.0405 | 0.0713 | 0.0087 | 0.0359 | 0.0221 | 0.0241 | 0.1025 | 0.0467 | 0.0113 |
| Vacuole Periphery | 0.0215 | 0.0066 | 0.0189 | 0.0268 | 0.0345 | 0.011 | 0.0087 | 0.0005 | 0.0049 | 0.0037 | 0.0149 | 0.0024 | 0.0058 | 0.0013 | 0.0038 | 0.0199 | 0.0071 | 0.0029 |
Sequencing Data
| R1 | R2 | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
| Gene Expression | 19.315 | 22.8598 | 26.1663 | 30.1475 | 18.5175 | 24.4703 | 27.478 | 36.2619 | 34.3916 | 27.7114 |
| Translational Efficiency | 0.5808 | 0.4792 | 0.6171 | 0.5059 | 0.6037 | 0.6282 | 0.6031 | 0.4925 | 0.5649 | 0.5889 |
Hit Data
| Dataset | Hit |
|---|---|
| Protein Concentration | ✘ |
| Protein Localization | ✘ |
| Gene Expression | ✔ |
| Translational Efficiency | ✘ |
Micrographs
Cell Count
| R1 | R2 | R1 & R2 | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
| 157 | 678 | 153 | 1178 | 1434 | 2045 | 470 | 1941 | 1591 | 2723 | 623 | 3119 |
Protein Abundance
| R1 | R2 | R1 & R2 | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
| Mean | 3684.04 | 3450.44 | 5307.17 | 4100.91 | 3370.36 | 3570.24 | 4709.36 | 4640.57 | 3401.31 | 3540.41 | 4856.17 | 4436.75 |
| Standard Deviation | 714.32 | 736.69 | 848.19 | 995.00 | 704.16 | 771.65 | 742.93 | 954.44 | 711.35 | 764.85 | 811.97 | 1004.63 |
| Intensity Change Log 2 | — | -0.094508 | 0.526654 | 0.154655 | — | 0.083118 | 0.482628 | 0.461399 | — | -0.006913 | 0.505788 | 0.309354 |
Localization Score (DeepLoc)
| R1 | R2 | R1 & R2 | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
| Actin | 0.000100 | 0.000103 | 0.000290 | 0.000205 | 0.000037 | 0.000119 | 0.000149 | 0.000324 | 0.000043 | 0.000115 | 0.000184 | 0.000279 |
| Bud Neck | 0.000519 | 0.000713 | 0.008814 | 0.001756 | 0.000145 | 0.000453 | 0.005714 | 0.000961 | 0.000181 | 0.000518 | 0.006476 | 0.001261 |
| Bud Site | 0.004753 | 0.003417 | 0.003491 | 0.012137 | 0.002029 | 0.003553 | 0.007226 | 0.009949 | 0.002298 | 0.003519 | 0.006309 | 0.010775 |
| Cell Periphery | 0.033479 | 0.008634 | 0.061753 | 0.010808 | 0.005009 | 0.002456 | 0.006691 | 0.028831 | 0.007819 | 0.003994 | 0.020213 | 0.022024 |
| Cytoplasm | 0.671902* | 0.838225* | 0.313053* | 0.656946* | 0.847918* | 0.890436* | 0.509585* | 0.739018* | 0.830549* | 0.877436* | 0.461320* | 0.708020* |
| Cytoplasmic Foci | 0.013595 | 0.006845 | 0.027351 | 0.005921 | 0.010820 | 0.008406 | 0.012948 | 0.002755 | 0.011094 | 0.008018 | 0.016485 | 0.003951 |
| Eisosomes | 0.000002 | 0.000001 | 0.000015 | 0.000003 | 0.000001 | 0.000001 | 0.000003 | 0.000004 | 0.000001 | 0.000001 | 0.000006 | 0.000003 |
| Endoplasmic Reticulum | 0.000652 | 0.000781 | 0.000259 | 0.000278 | 0.001326 | 0.000451 | 0.000331 | 0.000365 | 0.001259 | 0.000533 | 0.000313 | 0.000332 |
| Endosome | 0.001050 | 0.000744 | 0.001252 | 0.002188 | 0.000633 | 0.000761 | 0.001663 | 0.000592 | 0.000674 | 0.000757 | 0.001562 | 0.001195 |
| Golgi | 0.093357 | 0.038786 | 0.061351 | 0.013205 | 0.041120 | 0.028260 | 0.032641 | 0.008251 | 0.046275 | 0.030881 | 0.039692 | 0.010122 |
| Lipid Particles | 0.018994 | 0.011563 | 0.044953 | 0.012141 | 0.005928 | 0.003510 | 0.013281 | 0.020926 | 0.007218 | 0.005515 | 0.021059 | 0.017608 |
| Mitochondria | 0.000084 | 0.000145 | 0.000155 | 0.000561 | 0.000272 | 0.000138 | 0.000105 | 0.001139 | 0.000253 | 0.000140 | 0.000118 | 0.000921 |
| Mitotic Spindle | 0.001429 | 0.001009 | 0.002889 | 0.002508 | 0.000586 | 0.001182 | 0.004220 | 0.000505 | 0.000669 | 0.001139 | 0.003893 | 0.001262 |
| None | 0.001685 | 0.002076 | 0.001563 | 0.001528 | 0.001224 | 0.001283 | 0.000850 | 0.000795 | 0.001269 | 0.001481 | 0.001025 | 0.001072 |
| Nuclear Periphery | 0.000368 | 0.000266 | 0.005409 | 0.000258 | 0.000159 | 0.000208 | 0.001435 | 0.000189 | 0.000180 | 0.000222 | 0.002411 | 0.000215 |
| Nuclear Periphery Foci | 0.012568 | 0.010987 | 0.030926 | 0.003986 | 0.007329 | 0.006592 | 0.020633 | 0.003596 | 0.007846 | 0.007687 | 0.023161 | 0.003743 |
| Nucleolus | 0.000050 | 0.000107 | 0.000153 | 0.000301 | 0.000030 | 0.000057 | 0.000186 | 0.000204 | 0.000032 | 0.000069 | 0.000178 | 0.000241 |
| Nucleus | 0.005966 | 0.012056 | 0.038067 | 0.012544 | 0.001923 | 0.005446 | 0.021945 | 0.005846 | 0.002322 | 0.007092 | 0.025904 | 0.008376 |
| Peroxisomes | 0.000022 | 0.000021 | 0.000017 | 0.000138 | 0.000031 | 0.000025 | 0.000026 | 0.000126 | 0.000030 | 0.000024 | 0.000024 | 0.000131 |
| Vacuole | 0.060285 | 0.037089 | 0.340657* | 0.236225* | 0.026915 | 0.026045 | 0.311219* | 0.163195 | 0.030208 | 0.028794 | 0.318448* | 0.190778 |
| Vacuole Periphery | 0.079141 | 0.026430 | 0.057583 | 0.026362 | 0.046566 | 0.020616 | 0.049150 | 0.012431 | 0.049780 | 0.022063 | 0.051221 | 0.017692 |
Localization Changes (T-score)
| R1 | R2 | R1 & R2 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
| Actin | -0.31 | -4.51 | -2.60 | -2.76 | 2.49 | -2.20 | -6.92 | -2.11 | -1.38 | -0.87 | -2.51 | -8.96 | -2.71 | -1.66 | -0.51 |
| Bud Neck | -1.37 | -5.18 | -8.49 | -6.98 | 4.13 | -4.88 | -10.08 | -9.71 | -5.04 | 8.89 | -5.77 | -11.18 | -13.82 | -9.05 | 9.25 |
| Bud Site | 0.80 | 0.65 | -5.16 | -5.72 | -5.91 | -3.07 | -4.57 | -7.61 | -5.43 | -0.67 | -2.99 | -4.68 | -10.42 | -7.83 | -2.42 |
| Cell Periphery | 5.04 | -3.61 | 5.00 | -0.32 | 9.36 | 5.05 | -2.35 | -15.68 | -18.10 | -14.02 | 5.16 | -6.69 | -11.41 | -16.97 | -0.72 |
| Cytoplasm | -5.72 | 10.27 | 1.14 | 14.76 | -13.11 | -4.98 | 21.69 | 12.62 | 19.16 | -14.54 | -5.67 | 26.15 | 15.70 | 25.57 | -17.64 |
| Cytoplasmic Foci | 1.90 | -2.94 | 1.98 | 0.33 | 5.05 | 2.09 | -3.30 | 8.31 | 9.26 | 8.72 | 2.94 | -4.84 | 7.24 | 7.29 | 9.35 |
| Eisosomes | 1.40 | -6.72 | -1.28 | -5.49 | 6.53 | -1.62 | -8.80 | -4.04 | -3.76 | 0.79 | -1.15 | -10.42 | -5.27 | -4.98 | 4.92 |
| Endoplasmic Reticulum | -0.71 | 4.08 | 4.13 | 3.28 | 0.35 | 5.01 | 5.87 | 5.61 | 1.42 | -0.66 | 4.52 | 6.22 | 6.16 | 3.52 | -0.27 |
| Endosome | 1.75 | -1.43 | -2.38 | -3.29 | -1.74 | -1.77 | -7.03 | 0.34 | 1.68 | 6.66 | -1.43 | -7.67 | -2.93 | -2.14 | 2.83 |
| Golgi | 5.15 | 3.27 | 8.11 | 8.27 | 11.30 | 4.40 | 4.06 | 13.41 | 11.85 | 11.69 | 5.42 | 3.43 | 15.02 | 14.12 | 15.22 |
| Lipid Particles | 2.17 | -4.66 | 2.30 | -0.10 | 6.65 | 3.22 | -6.56 | -10.62 | -13.49 | -3.81 | 1.95 | -8.70 | -9.54 | -12.30 | 2.59 |
| Mitochondria | -1.04 | -3.45 | -3.39 | -2.64 | -2.68 | 2.87 | 3.24 | -1.53 | -1.76 | -1.78 | 2.59 | 2.89 | -1.85 | -2.16 | -2.17 |
| Mitotic Spindle | 1.13 | -2.66 | -1.30 | -1.77 | 0.59 | -3.17 | -3.52 | 0.35 | 3.00 | 3.54 | -3.05 | -4.02 | -1.87 | -0.21 | 3.24 |
| None | -1.22 | 0.59 | 0.71 | 1.48 | 0.41 | -0.32 | 2.07 | 1.93 | 2.45 | 0.38 | -1.21 | 1.51 | 1.11 | 2.18 | 0.11 |
| Nuclear Periphery | 1.87 | -9.14 | 1.60 | -0.05 | 9.31 | -1.60 | -8.30 | -2.08 | 0.49 | 8.09 | -1.74 | -11.84 | -2.20 | 0.09 | 11.60 |
| Nuclear Periphery Foci | 0.67 | -5.73 | 4.36 | 6.95 | 10.64 | 0.72 | -6.50 | 5.26 | 5.25 | 8.64 | 0.03 | -8.93 | 6.34 | 8.04 | 12.01 |
| Nucleolus | -1.29 | -8.61 | -4.89 | -2.92 | -2.71 | -1.66 | -14.08 | -2.69 | -2.15 | 0.15 | -2.27 | -16.33 | -4.71 | -3.57 | -0.92 |
| Nucleus | -2.50 | -6.88 | -5.73 | -2.07 | 4.88 | -8.33 | -10.85 | -8.16 | -2.09 | 8.68 | -8.07 | -13.17 | -12.56 | -4.26 | 9.28 |
| Peroxisomes | 0.03 | 0.52 | -4.15 | -4.18 | -4.31 | 0.49 | -0.28 | -7.49 | -7.24 | -7.46 | 0.69 | 0.22 | -7.55 | -7.63 | -7.75 |
| Vacuole | 2.45 | -12.87 | -16.30 | -25.29 | 4.39 | -0.07 | -22.66 | -27.30 | -27.50 | 11.13 | 0.20 | -26.81 | -36.28 | -37.47 | 10.95 |
| Vacuole Periphery | 3.92 | 1.82 | 4.26 | 1.23 | 5.34 | 8.62 | 1.30 | 12.60 | 5.60 | 8.45 | 9.22 | 1.48 | 11.94 | 4.23 | 9.42 |
| Standard name | |
|---|---|
| Human Ortholog | |
| Description | Mitochondrial RNA polymerase; single subunit enzyme similar to those of T3 and T7 bacteriophages; requires a specificity subunit encoded by MTF1 for promoter recognition; Mtf1p interacts with and stabilizes the Rpo41p-promoter complex, enhancing DNA bending and melting to facilitate pre-initiation open complex formation; Rpo41p also synthesizes RNA primers for mitochondrial DNA replication |
| Localization | |
| Cell Percentages | cytoplasm (100%) |
| Cell Cycle Regulation | No |
| Subcompartmental Group | cytoplasm-2 |
Rpo41
Rpo41
Nearest neighbours ( genes)
correlation threshold
0.80
Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)
Endocytosis
| Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
|---|---|---|---|---|
| 37℃ | ||||
| RT |
Cell Cycle Omics
CYCLoPs (Rpo41-GFP)
| Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
|---|
| Gene | Images |
|---|
| Gene | Images |
|---|
Images are not yet available
Images are not yet available
