Standard name
Human Ortholog
Description Phosphomannomutase; involved in synthesis of GDP-mannose and dolichol-phosphate-mannose; required for folding and glycosylation of secretory proteins in the ER lumen
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery – 0 0 0 0 0 0 0 – 0.05 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm – 0.96 0.98 0.97 0.97 0.99 0.95 0.89 – 0.84 0.89 0.86 0.98 0.96 0.98 0.98 0.99 0.98 0.86 0.91 0.96 0.91 0.91 0.91
Endoplasmic Reticulum – 0 0 0 0 0 0 0 – 0.07 0 0.09 0 0 0 0 0 0 0.09 0 0 0 0 0
Endosome – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane – 0 0 0 0 0 0 0.06 – 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – 1 0 0 0 1 0 1 – 4 3 4 0 0 0 1 0 1 0 0 0 0 0 0
Bud – 4 0 0 2 2 4 4 – 2 1 3 3 4 2 0 0 0 0 0 0 1 1 3
Bud Neck – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery – 5 2 4 4 4 10 13 – 20 16 18 9 7 12 8 7 4 0 0 0 0 0 0
Cytoplasm – 160 171 228 255 338 499 383 – 362 310 371 279 286 331 387 275 235 6 164 169 149 290 278
Endoplasmic Reticulum – 4 1 5 3 2 14 15 – 30 10 38 3 9 4 13 4 3 0 3 1 1 3 1
Endosome – 0 0 0 0 0 0 0 – 0 0 0 1 0 0 0 0 0 0 2 0 0 1 1
Golgi – 0 0 0 0 0 0 2 – 2 1 1 1 0 0 0 0 0 0 0 0 0 0 0
Mitochondria – 0 0 0 0 1 2 3 – 3 11 19 0 0 0 1 0 0 0 1 0 1 0 0
Nucleus – 1 1 1 0 0 2 3 – 2 1 0 0 2 1 1 1 0 0 1 1 1 3 5
Nuclear Periphery – 0 0 0 0 0 0 0 – 0 0 1 0 0 0 0 0 0 0 1 0 0 1 1
Nucleolus – 0 0 0 0 0 0 0 – 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane – 1 1 1 4 1 8 24 – 30 6 7 1 6 2 3 3 3 0 1 1 6 12 9
Unique Cell Count 166 174 234 263 343 525 428 430 349 433 285 299 338 394 279 239 7 181 176 165 319 306
Labelled Cell Count 176 176 239 268 349 539 448 456 359 462 297 314 352 414 290 246 7 181 176 165 319 306


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) – 40.8 27.4 30.6 25.9 21.0 19.6 19.2 – 19.0 17.9 17.7 40.9 44.6 41.9 38.3 41.3 39.7 39.3 41.9 41.6
Std Deviation (1e-4) – 11.2 5.1 6.4 5.9 4.5 4.3 4.4 – 4.6 4.6 4.3 9.4 12.0 10.4 9.4 11.4 11.8 8.8 9.2 9.3
Intensity Change (Log2) – – – 0.16 -0.08 -0.38 -0.48 -0.51 – -0.53 -0.62 -0.62 0.58 0.7 0.61 0.49 0.6 0.54 0.52 0.62 0.6


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 – 2.1 2.0 1.9 0 0 0 0 0 0
Cytoplasm -0.6 -0.9 0.2 -1.9 -3.6 – -4.9 -3.7 -4.5 -0.3 -1.5 -0.3 0 0.2 0
Endoplasmic Reticulum 0 0 0 0 0 – 3.2 0 3.7 0 0 0 0 0 0
Endosome 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 – 0 0 2.8 0 0 0 0 0 0
Nucleus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 2.8 – 3.2 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 36.5023 49.1423 47.7014 44.9366 38.9674 45.0655 37.6979 48.3889 48.2695 51.2481 45.5752 44.1876 41.3571 50.1422 50.9173 48.7888 52.0557 47.4839
Actin 0.0084 0.0001 0.0004 0 0.0006 0.0002 0.0065 0.0003 0.0031 0.0002 0.0027 0.0004 0.0074 0.001 0.004 0 0 0.0001
Bud 0.0006 0 0.0001 0 0.0002 0.0004 0.0013 0.0001 0.0002 0 0.0003 0.0004 0.0002 0.0001 0.0002 0 0 0
Bud Neck 0.0003 0.0001 0.0003 0.0001 0.0016 0.0005 0.0033 0.0001 0.0003 0.0003 0.0005 0.0004 0.0003 0.0001 0.0002 0.0001 0.0002 0.0002
Bud Periphery 0.0004 0 0.0002 0 0.0001 0.0001 0.0005 0 0.0001 0 0.0006 0.0003 0.0002 0.0001 0.0001 0 0 0
Bud Site 0.0006 0 0.0001 0 0.0002 0 0.0042 0 0.0002 0 0.0006 0.0003 0.0005 0.0005 0.0007 0 0 0
Cell Periphery 0.0002 0 0.0003 0 0.0001 0 0.0003 0 0.0001 0 0.0002 0.0002 0.0002 0.0001 0.0001 0 0 0
Cytoplasm 0.8897 0.9945 0.9834 0.9808 0.867 0.9658 0.9146 0.9858 0.9762 0.989 0.9035 0.9585 0.9249 0.9826 0.9706 0.984 0.9935 0.9708
Cytoplasmic Foci 0.0158 0.0001 0.0016 0.0001 0.0382 0.0044 0.0181 0.0002 0.0048 0.0002 0.0074 0.0038 0.0033 0.001 0.0027 0.0001 0.0001 0.0009
Eisosomes 0.0001 0 0 0 0.0001 0 0.0001 0 0 0 0.0001 0 0.0003 0 0.0001 0 0 0
Endoplasmic Reticulum 0.0037 0.0001 0.0004 0.0001 0.0021 0.0004 0.0018 0.0001 0.0016 0.0001 0.0092 0.0015 0.0017 0.0006 0.0008 0 0.0001 0.0003
Endosome 0.0071 0 0.0003 0 0.0059 0.0005 0.0071 0.0001 0.0014 0 0.0124 0.0037 0.0067 0.0008 0.0013 0 0 0.0014
Golgi 0.0012 0 0.0001 0 0.0008 0.0001 0.003 0.0001 0.0004 0 0.0017 0.0006 0.0019 0.0004 0.0007 0 0 0
Lipid Particles 0.0047 0 0.0002 0 0.0149 0.0025 0.0081 0 0.0008 0 0.0053 0.0008 0.0058 0.0004 0.0021 0 0 0
Mitochondria 0.0035 0 0.0004 0 0.0028 0.0003 0.0017 0.0098 0.0007 0 0.029 0.0117 0.0062 0.002 0.001 0 0 0
None 0.0047 0.0001 0.0014 0.0001 0.008 0.0009 0.0035 0.0002 0.0026 0.0001 0.0119 0.0019 0.0025 0.0008 0.001 0.0001 0.0001 0.0003
Nuclear Periphery 0.0101 0.0006 0.0011 0.0012 0.0087 0.0025 0.0032 0.0004 0.0011 0.0009 0.0032 0.0013 0.007 0.0015 0.0019 0.0008 0.0007 0.0055
Nucleolus 0.0015 0 0.0004 0 0.003 0.0024 0.0007 0 0.0004 0 0.0003 0.0009 0.0007 0.0002 0.001 0 0 0.0003
Nucleus 0.03 0.004 0.0074 0.0172 0.0126 0.0151 0.011 0.0026 0.0038 0.0089 0.0039 0.0091 0.0229 0.006 0.0077 0.0148 0.0051 0.0172
Peroxisomes 0.0019 0 0 0 0.001 0.0002 0.0039 0 0.0002 0 0.0003 0.0002 0.0015 0.0002 0.0008 0 0 0
Punctate Nuclear 0.0076 0 0.0006 0.0001 0.0084 0.0029 0.0022 0.0001 0.0007 0.0001 0.0016 0.0006 0.0014 0.0005 0.002 0 0 0.0023
Vacuole 0.0051 0.0001 0.001 0.0001 0.0106 0.0005 0.0038 0.0001 0.001 0 0.0038 0.0028 0.0031 0.0008 0.0008 0 0.0001 0.0004
Vacuole Periphery 0.0029 0 0.0002 0 0.013 0.0002 0.001 0 0.0004 0 0.0018 0.0007 0.0013 0.0003 0.0004 0 0 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 754.3536 751.7089 614.2502 461.8345 659.2036 726.5464 658.0382 511.2241 456.3172 523.0546
Translational Efficiency 1.6128 1.6477 1.4568 2.2008 1.8771 1.5962 1.6067 1.6887 1.5933 1.7837

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Phosphomannomutase; involved in synthesis of GDP-mannose and dolichol-phosphate-mannose; required for folding and glycosylation of secretory proteins in the ER lumen
Localization
Cell Percentages cytoplasm (98%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-7

Sec53

Sec53


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Sec53-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available