Standard name
Human Ortholog
Description Integral membrane component of ER-derived COPII-coated vesicles; functionS in ER to Golgi transport; EMP47 has a paralog, EMP46, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0.06 0.11 0.19 0.2 0.19 – 0.28 0.34 0.35 0.06 0.06 0 0.1 0.29 0.1 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.1 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.05 0.1 0 0 0 0 0 0
Mitochondria 0 0.07 0.05 0 0.05 0 0 0 – 0 0.05 0 0 0 0 0.05 0.07 0 0 0 0 0 0 0
Nucleus 0.15 0.18 0.06 0 0.05 0.11 0.07 0 – 0 0.05 0 0.07 0.07 0.09 0.05 0.07 0.05 0.09 0.05 0 0.08 0.07 0.06
Nuclear Periphery 0.06 0 0.11 0.21 0.05 0.06 0.12 0.14 – 0.06 0 0.06 0 0 0 0 0 0 0 0 0.06 0 0 0
Nucleolus 0 0 0.08 0.08 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.82 0.78 0.83 0.85 0.79 0.69 0.74 0.71 – 0.66 0.63 0.6 0.91 0.9 0.89 0.85 0.71 0.9 0.82 0.92 0.86 0.84 0.83 0.8
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 1 0 0 2 1 5 – 0 1 3 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 1 0 0 0 0 0 1 – 2 0 2 0 0 0 0 0 0 0 0 2 2 3 9
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 1 3 3 6 15 22 – 17 10 20 0 0 1 0 0 0 0 0 0 0 0 0
Cytoplasm 4 0 17 23 37 82 128 135 – 137 135 172 26 26 10 2 12 2 0 0 2 0 0 3
Endoplasmic Reticulum 0 0 6 1 2 0 7 27 – 9 3 7 1 0 1 2 1 0 0 0 0 0 1 1
Endosome 0 0 7 11 3 0 0 3 – 0 4 2 1 2 5 0 0 0 1 0 7 2 12 14
Golgi 3 0 4 7 0 0 2 1 – 0 0 3 13 7 12 0 2 2 0 0 2 1 1 4
Mitochondria 3 7 18 7 17 17 16 9 – 19 18 13 0 0 2 1 3 0 0 0 4 2 4 4
Nucleus 35 19 23 10 19 50 45 32 – 20 21 20 32 32 44 1 3 1 21 5 8 23 38 32
Nuclear Periphery 14 4 40 77 17 24 74 103 – 29 17 32 13 11 16 0 0 0 8 0 23 5 17 18
Nucleolus 5 0 30 28 14 10 10 5 – 3 4 1 5 5 9 0 0 0 9 0 4 9 18 15
Peroxisomes 0 0 0 0 1 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 1 0 0 0 – 0 0 0 0 0 0 0 0 0 1 0 0 0 1 1
Vac/Vac Membrane 190 80 315 316 278 302 469 514 – 326 249 299 413 413 430 17 30 18 200 98 347 258 480 441
Unique Cell Count 231 103 379 373 351 436 637 722 495 397 498 456 461 481 20 42 20 244 108 405 308 582 552
Labelled Cell Count 254 111 462 483 392 493 767 857 562 462 574 504 496 530 23 51 23 244 108 405 308 582 552


Vacuole Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 17.4 15.7 16.7 16.2 16.1 14.1 14.4 13.9 – 11.9 12.1 12.4 15.5 15.5 14.7 12.3 14.0 17.7 18.6 18.6 19.4
Std Deviation (1e-4) 4.7 5.7 5.6 5.1 5.5 5.2 5.2 4.6 – 4.2 4.2 3.9 4.2 4.2 3.7 4.4 5.1 5.7 5.7 6.2 6.4
Intensity Change (Log2) – – – -0.04 -0.05 -0.24 -0.21 -0.26 – -0.49 -0.47 -0.42 -0.1 -0.1 -0.18 -0.43 -0.25 0.09 0.16 0.16 0.22


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 2.6 3.1 – 3.3 2.7 3.6 0 0 0 0 0 0
Cytoplasm 1.0 3.1 6.2 6.9 6.5 – 8.9 10.4 10.7 0.8 0.8 -2.0 0 0 0
Endoplasmic Reticulum 0 0 0 0 2.0 – 0.3 0 -0.2 0 0 0 0 0 0
Endosome 1.0 0 0 0 0 – 0 -1.0 0 0 0 -1.0 0 0 0
Golgi 0.9 0 0 0 0 – 0 0 0 1.8 0 1.6 0 0 0
Mitochondria -2.2 0.1 -0.6 -1.9 -3.6 – -0.7 -0.1 -1.7 -4.7 -4.7 -4.2 0 0 0
Nucleus -2.3 -0.4 2.7 0.6 -1.2 – -1.4 -0.5 -1.4 0.6 0.5 1.7 0 0 0
Nuclear Periphery 3.8 -2.9 -2.7 0.5 1.7 – -2.6 -3.3 -2.2 -4.5 -4.9 -4.3 0 0 0
Nucleolus -0.2 -2.2 -3.7 -5.0 -6.5 – -5.6 -4.7 -6.1 -4.9 -4.9 -4.2 0 0 0
Peroxisomes 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Vacuole 0.6 -1.4 -4.6 -3.5 -4.4 – -5.7 -6.4 -7.4 3.2 2.7 2.7 0.2 -1.9 0.8

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 10.9939 15.4974 14.0542 12.8686 12.2723 13.9908 11.9477 18.0569 15.2473 14.3973 13.781 15.4531 12.9334 18.2484 15.5484 15.8003 14.7995 15.9997
Actin 0.0026 0 0.0048 0 0.0102 0 0.0161 0 0.0002 0.0024 0.0031 0.0053 0.0003 0 0.0019 0 0 0
Bud 0.0009 0.0001 0.0006 0 0.0002 0 0.0002 0 0.0002 0.0001 0.0002 0 0.0001 0 0.0003 0 0.0003 0
Bud Neck 0.0011 0.0001 0.0016 0 0.0006 0 0.0003 0 0.0003 0.0001 0.001 0.0001 0.0002 0 0.0005 0 0.0001 0
Bud Periphery 0.0026 0.0001 0.001 0 0.0004 0.0001 0.0006 0.0001 0.001 0.0002 0.0003 0.0001 0.0003 0.0001 0.0006 0.0001 0.0005 0.0001
Bud Site 0.0019 0.0001 0.0069 0 0.0003 0 0.0006 0 0.0004 0.0006 0.0018 0 0.0004 0 0.0036 0 0.0002 0
Cell Periphery 0.0018 0.0012 0.0015 0.0004 0.0009 0.0003 0.0015 0.0008 0.0018 0.0004 0.0004 0.0007 0.0016 0.0007 0.0018 0.0008 0.0009 0.0008
Cytoplasm 0.0056 0.0066 0.012 0.0001 0.0017 0.0009 0.0011 0.0013 0.0005 0.0016 0.0002 0 0.0003 0.0012 0.0003 0 0.0003 0.0008
Cytoplasmic Foci 0.0051 0.001 0.0041 0 0.002 0.0001 0.0071 0 0.0024 0.0081 0.0069 0.0001 0.0061 0.0001 0.0017 0 0.0008 0.0001
Eisosomes 0.0001 0 0.0008 0 0.0001 0 0.0001 0 0.0003 0.0001 0.0001 0 0 0 0 0 0 0
Endoplasmic Reticulum 0.0383 0.0109 0.0021 0 0.0022 0.0031 0.001 0.0001 0.0003 0.0027 0.0001 0.0001 0.0004 0.0017 0.0002 0.0001 0.0002 0.0015
Endosome 0.0193 0.0036 0.0095 0.0036 0.0189 0.0036 0.0164 0.0023 0.0143 0.0211 0.008 0.0025 0.0082 0.0069 0.0051 0.0041 0.0062 0.0039
Golgi 0.0035 0.0001 0.0011 0 0.0021 0 0.0038 0 0.0026 0.0023 0.006 0.0005 0.0008 0.0002 0.0009 0 0.0001 0.0001
Lipid Particles 0.0092 0.0009 0.0024 0.0001 0.0035 0.0004 0.0103 0.0004 0.0056 0.0112 0.0058 0.0012 0.0076 0.0002 0.0047 0.0002 0.001 0.0018
Mitochondria 0.0099 0.0005 0.0053 0.0001 0.0016 0.0002 0.0145 0.0004 0.0199 0.0032 0.0233 0.0003 0.0116 0.0007 0.0102 0.0006 0.0021 0.0013
None 0.0011 0 0.0012 0 0.0039 0 0.0025 0 0.006 0.0011 0.0001 0 0.0002 0 0.0007 0 0.0001 0.0001
Nuclear Periphery 0.0098 0.0061 0.0038 0.0003 0.0209 0.0144 0.0072 0.0027 0.0021 0.0086 0.0009 0.0041 0.0026 0.0097 0.0041 0.0003 0.0008 0.0045
Nucleolus 0.0062 0.0053 0.0043 0.0002 0.0061 0.0001 0.0034 0.0002 0.0057 0.0008 0.0003 0.0006 0.0021 0.0002 0.0065 0.0002 0.0036 0.0026
Nucleus 0.0135 0.0062 0.0098 0.0003 0.0099 0.001 0.0035 0.0005 0.0028 0.002 0.0004 0.0006 0.0029 0.0012 0.0031 0.0003 0.0022 0.0034
Peroxisomes 0.0031 0 0.0019 0 0.0006 0 0.0032 0 0.0004 0.0007 0.0119 0.0001 0.0072 0 0.0114 0 0.0002 0
Punctate Nuclear 0.0012 0 0.0054 0 0.01 0.0001 0.0046 0 0.0003 0.0077 0.0001 0 0.0033 0 0.0007 0 0 0
Vacuole 0.3435 0.3932 0.3536 0.4257 0.3404 0.3039 0.2881 0.3013 0.2627 0.3013 0.286 0.2176 0.3381 0.3074 0.2894 0.3763 0.3433 0.2306
Vacuole Periphery 0.5199 0.5639 0.5661 0.5692 0.5636 0.6717 0.6142 0.6897 0.6703 0.6235 0.643 0.7661 0.6057 0.6699 0.6522 0.6171 0.637 0.7483

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 58.139 53.0785 48.6862 56.6781 50.2532 41.2105 57.9536 62.1309 54.8846 66.9927
Translational Efficiency 1.3089 1.2965 1.4916 1.2216 1.2921 1.7937 1.4433 1.2152 1.3607 1.1686

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
139 918 2867 1217 1371 1887 587 1761 1510 2805 3454 2978

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1205.31 1470.66 1612.65 1701.70 1269.30 1412.87 1624.44 1672.90 1263.41 1431.78 1614.65 1684.67
Standard Deviation 192.37 388.00 251.29 377.67 272.94 316.51 230.55 313.71 267.19 342.63 247.92 341.60
Intensity Change Log 2 — 0.287060 0.420029 0.497572 — 0.154596 0.355909 0.398318 — 0.220635 0.387496 0.447515

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000292 0.000116 0.000014 0.000122 0.000019 0.000052 0.000080 0.000042 0.000044 0.000073 0.000025 0.000075
Bud Neck 0.005013 0.001023 0.000390 0.000889 0.000082 0.000588 0.002252 0.000631 0.000536 0.000731 0.000706 0.000737
Bud Site 0.012837 0.005049 0.000214 0.002647 0.000445 0.001754 0.000999 0.001027 0.001586 0.002832 0.000348 0.001689
Cell Periphery 0.000521 0.000992 0.000302 0.001627 0.001442 0.000901 0.000240 0.000937 0.001357 0.000931 0.000292 0.001219
Cytoplasm 0.002493 0.004441 0.000027 0.000527 0.000685 0.001099 0.000080 0.000063 0.000851 0.002192 0.000036 0.000253
Cytoplasmic Foci 0.009740 0.004203 0.000226 0.001516 0.000487 0.001417 0.001450 0.000608 0.001339 0.002329 0.000434 0.000979
Eisosomes 0.000026 0.000013 0.000027 0.000015 0.000003 0.000020 0.000064 0.000025 0.000005 0.000018 0.000033 0.000021
Endoplasmic Reticulum 0.002782 0.000496 0.000186 0.000463 0.000632 0.000391 0.000242 0.000410 0.000830 0.000425 0.000196 0.000431
Endosome 0.045249 0.006545 0.000461 0.005929 0.000896 0.004233 0.002657 0.004083 0.004979 0.004990 0.000834 0.004837
Golgi 0.009666 0.007788 0.000298 0.021239 0.000935 0.002830 0.001564 0.008045 0.001739 0.004452 0.000513 0.013437
Lipid Particles 0.000629 0.000452 0.000870 0.000992 0.000175 0.000833 0.000689 0.001239 0.000217 0.000709 0.000839 0.001138
Mitochondria 0.014820 0.004669 0.001969 0.004027 0.000596 0.003454 0.003885 0.005332 0.001905 0.003852 0.002294 0.004799
Mitotic Spindle 0.001207 0.000369 0.000031 0.001816 0.000021 0.000424 0.000266 0.000710 0.000130 0.000406 0.000071 0.001162
None 0.000113 0.001092 0.000184 0.000223 0.000140 0.000306 0.000213 0.000228 0.000137 0.000564 0.000189 0.000226
Nuclear Periphery 0.001680 0.000590 0.002100 0.001232 0.000083 0.000688 0.002652 0.001776 0.000230 0.000656 0.002194 0.001553
Nuclear Periphery Foci 0.001401 0.001193 0.000873 0.001451 0.000097 0.000995 0.001859 0.001743 0.000217 0.001060 0.001040 0.001623
Nucleolus 0.005354 0.002756 0.003936 0.002006 0.000477 0.003009 0.014357 0.002387 0.000926 0.002926 0.005707 0.002231
Nucleus 0.006397 0.002093 0.001560 0.000729 0.000422 0.001094 0.004233 0.001096 0.000972 0.001421 0.002014 0.000946
Peroxisomes 0.002732 0.000574 0.000071 0.000101 0.000035 0.000243 0.000412 0.000081 0.000283 0.000352 0.000129 0.000089
Vacuole 0.599988* 0.267237* 0.236429* 0.384914* 0.256970* 0.279902* 0.454871* 0.410889* 0.288546* 0.275757* 0.273553* 0.400274*
Vacuole Periphery 0.277061* 0.688308* 0.749833* 0.567537* 0.735359* 0.695766* 0.506935* 0.558648* 0.693171* 0.693325* 0.708553* 0.562281*

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 1.00 1.60 0.92 -0.23 -2.77 -2.03 -1.91 -2.09 0.68 1.20 -1.38 1.03 -1.31 -0.15 -2.87
Bud Neck 2.88 3.35 2.98 0.47 -3.47 -3.01 -4.87 -8.04 -0.29 3.58 -0.96 -1.00 -1.31 -0.13 -0.36
Bud Site 1.71 2.81 2.26 1.87 -4.96 -3.33 -2.77 -3.08 1.75 -0.16 -1.92 2.81 -0.21 2.19 -5.97
Cell Periphery -1.38 0.73 -2.76 -1.96 -4.86 1.89 4.59 1.80 -0.22 -6.16 1.66 4.46 0.51 -1.78 -7.11
Cytoplasm -1.12 2.28 1.66 2.74 -2.68 -0.96 2.36 2.44 2.81 0.82 -2.38 3.23 2.01 3.64 -2.79
Cytoplasmic Foci 1.54 2.76 2.37 2.71 -4.47 -2.79 -1.28 -0.47 3.34 1.18 -1.91 2.18 0.85 3.66 -3.08
Eisosomes 2.59 -0.19 2.10 -0.65 3.32 -4.60 -6.86 -5.56 -0.94 4.03 -4.82 -10.01 -6.05 -0.91 3.39
Endoplasmic Reticulum 1.40 1.60 1.42 0.35 -5.42 2.59 5.29 2.73 -0.28 -3.56 2.38 3.90 2.39 -0.13 -7.14
Endosome 4.82 5.63 4.92 0.47 -8.00 -5.29 -2.64 -7.72 0.21 -1.86 0.01 5.02 0.17 0.22 -10.58
Golgi 0.54 2.96 -3.06 -5.51 -9.88 -3.85 -1.02 -6.82 -4.94 -5.81 -4.34 2.81 -10.33 -7.80 -12.25
Lipid Particles 1.06 -1.53 -1.47 -2.58 -0.63 -6.00 -6.76 -10.00 -2.64 -4.24 -6.31 -15.49 -8.84 -3.34 -2.76
Mitochondria 1.58 2.03 1.69 0.51 -2.67 -4.56 -2.59 -5.94 -1.93 -0.98 -2.43 -0.57 -3.56 -1.27 -3.94
Mitotic Spindle 0.78 1.39 -0.65 -2.00 -2.98 -1.25 -1.47 -1.95 -0.59 -1.16 -1.30 0.68 -3.14 -1.79 -3.38
None -2.82 -3.40 -3.35 2.50 -1.45 -3.13 -1.49 -1.87 2.51 -0.61 -3.48 -1.22 -2.07 2.90 -2.43
Nuclear Periphery 0.99 -0.38 0.40 -1.94 2.05 -4.16 -2.68 -7.80 -4.17 0.91 -2.78 -5.92 -6.83 -4.39 1.78
Nuclear Periphery Foci -0.12 0.89 -0.10 0.06 -1.72 -2.54 -2.87 -4.95 -1.56 0.17 -2.95 -4.73 -5.87 -1.20 -2.07
Nucleolus 1.61 0.93 2.07 0.78 2.36 -4.69 -7.03 -5.28 0.96 5.97 -4.45 -9.08 -3.46 1.26 5.68
Nucleus 1.58 1.82 2.13 2.20 2.72 -2.85 -3.58 -2.27 -0.01 2.88 -1.27 -2.85 0.06 1.61 3.52
Peroxisomes 2.51 3.15 3.11 2.81 -1.04 -3.95 -3.45 -1.86 2.96 3.00 -0.64 1.86 2.35 3.96 1.52
Vacuole 14.49 16.48 9.44 -11.89 -19.50 -2.76 -16.66 -18.41 -17.11 3.84 1.62 1.95 -14.31 -20.59 -22.16
Vacuole Periphery -18.80* -23.12* -13.62 10.76 22.34* 4.53 18.43 20.37 16.78 -4.31 0.00 -1.87 15.68 19.91 24.14*
Standard name
Human Ortholog
Description Integral membrane component of ER-derived COPII-coated vesicles; functionS in ER to Golgi transport; EMP47 has a paralog, EMP46, that arose from the whole genome duplication
Localization
Cell Percentages vacuolar membrane (69%)
Cell Cycle Regulation No
Subcompartmental Group vac memb-2

Emp47

Emp47


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Emp47-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available