Standard name | |
---|---|
Human Ortholog | |
Description | Nucleolar protein involved in the asymmetric localization of ASH1 mRNA; binds cooperatively with She2p to the E3 zip-code element in the 3’-UTR of the mRNA; constituent of 66S pre-ribosomal particles involved in pre-rRNA processing and nuclear export of the 60S subunit; may facilitate Rpl43p loading; required post-transcriptionally for efficient retrotransposition; absence results in decreased Ty1 Gag:GFP protein levels; relocalizes from nucleus to cytoplasm upon DNA replication stress |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 1 | 2 | 0 | 1 | 1 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 1 | 1 | 1 |
Bud | 0 | 5 | 3 | 0 | 0 | 3 | 0 | – | 0 | 2 | 6 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 4 | 4 | 1 | 1 | 8 | 7 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 3 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 0 | 1 | 1 | 0 | 6 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 34 | 90 | 86 | 94 | 112 | 115 | 226 | – | 292 | 371 | 276 | 374 | 173 | 210 | 284 | 65 | 45 | 63 | 1 | 8 | 5 | 17 | 44 | 65 |
Endoplasmic Reticulum | 4 | 5 | 2 | 0 | 1 | 1 | 0 | – | 1 | 0 | 1 | 0 | 1 | 1 | 1 | 20 | 17 | 17 | 2 | 2 | 3 | 3 | 2 | 2 |
Endosome | 30 | 26 | 19 | 13 | 19 | 1 | 5 | – | 2 | 4 | 1 | 4 | 1 | 0 | 0 | 8 | 19 | 12 | 38 | 41 | 19 | 17 | 12 | 12 |
Golgi | 2 | 0 | 1 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 3 | 4 | 2 | 1 | 0 | 1 | 1 |
Mitochondria | 47 | 51 | 3 | 9 | 4 | 37 | 7 | – | 18 | 13 | 21 | 29 | 0 | 0 | 0 | 7 | 2 | 6 | 10 | 10 | 6 | 5 | 8 | 8 |
Nucleus | 31 | 38 | 53 | 55 | 62 | 103 | 79 | – | 33 | 15 | 17 | 15 | 2 | 2 | 1 | 181 | 117 | 166 | 4 | 5 | 1 | 3 | 2 | 1 |
Nuclear Periphery | 5 | 4 | 2 | 2 | 0 | 0 | 0 | – | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 2 | 0 | 1 | 2 | 4 | 1 |
Nucleolus | 11 | 4 | 1 | 0 | 3 | 1 | 0 | – | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 1 | 1 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 1 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 3 | 8 | 10 | 0 | 6 | 0 | 0 | – | 0 | 2 | 0 | 1 | 0 | 0 | 2 | 3 | 0 | 2 | 5 | 6 | 0 | 4 | 4 | 3 |
Vac/Vac Membrane | 197 | 218 | 184 | 170 | 248 | 168 | 228 | – | 158 | 145 | 82 | 90 | 12 | 0 | 0 | 184 | 206 | 261 | 245 | 304 | 303 | 149 | 186 | 134 |
Unique Cell Count | 285 | 356 | 304 | 274 | 358 | 338 | 432 | 409 | 461 | 338 | 446 | 181 | 212 | 287 | 374 | 328 | 433 | 323 | 394 | 352 | 211 | 285 | 249 | |
Labelled Cell Count | 364 | 450 | 366 | 343 | 457 | 430 | 545 | 507 | 555 | 406 | 517 | 191 | 220 | 289 | 470 | 406 | 532 | 323 | 394 | 352 | 211 | 285 | 249 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.6 | 5.2 | 6.2 | 6.1 | 7.0 | 5.1 | 6.1 | – | 5.1 | 5.3 | 5.1 | 5.0 | 5.1 | 4.8 | 5.0 | 7.2 | 8.1 | 7.3 | 5.3 | 5.2 | 5.7 |
Std Deviation (1e-4) | 1.3 | 1.1 | 1.2 | 1.4 | 1.4 | 1.3 | 1.6 | – | 1.2 | 1.2 | 1.2 | 1.2 | 1.4 | 1.2 | 2.0 | 1.6 | 1.7 | 1.6 | 1.7 | 1.8 | 2.9 |
Intensity Change (Log2) | – | – | – | -0.02 | 0.18 | -0.27 | -0.02 | – | -0.26 | -0.23 | -0.28 | -0.31 | -0.26 | -0.35 | -0.29 | 0.22 | 0.39 | 0.25 | -0.23 | -0.25 | -0.1 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 1.6 | 0.8 | 1.6 | 6.5 | – | 11.4 | 14.4 | 13.6 | 15.3 | 14.4 | 16.0 | 17.7 | -3.4 | -4.5 | -4.6 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.4 | 3.3 | 2.8 |
Endosome | -0.8 | -0.5 | -4.3 | -3.8 | – | -4.5 | -4.3 | -4.3 | -4.2 | -3.1 | 0 | -4.3 | -2.7 | -0.2 | -2.3 |
Golgi | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 1.9 | 0 | 5.2 | 0 | – | 2.7 | 1.7 | 3.5 | 3.7 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0.8 | 0 | 3.8 | 0.3 | – | -3.8 | -6.7 | -5.0 | -6.6 | -5.5 | -6.0 | -7.2 | 8.4 | 5.2 | 6.1 |
Nuclear Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | -1.3 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | -2.2 | -2.3 | 0 | 0 |
Vacuole | 0.4 | 2.4 | -2.8 | -2.1 | – | -5.8 | -7.9 | -9.3 | -11.3 | -11.7 | -14.1 | -15.9 | -2.9 | 0.6 | -0.1 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 0.3783 | 1.1651 | 0.8802 | -0.1104 | 0.7252 | 0.9004 | 1.9857 | 3.4356 | 3.0599 | 3.1805 | 2.7989 | 3.313 | 3.704 | 5.0563 | 4.712 | 5.1696 | 4.8586 | 5.0624 |
Actin | 0.0219 | 0 | 0.0174 | 0.0482 | 0.0906 | 0.0066 | 0.0028 | 0.0001 | 0.0033 | 0 | 0.0007 | 0 | 0.0066 | 0 | 0.0002 | 0 | 0 | 0 |
Bud | 0.0011 | 0.0007 | 0.0004 | 0.0002 | 0.0006 | 0.0005 | 0.0003 | 0.0006 | 0.0026 | 0.0002 | 0.0009 | 0.0004 | 0.0003 | 0.0005 | 0.0009 | 0.0004 | 0.0006 | 0.0003 |
Bud Neck | 0.0021 | 0.0008 | 0.0007 | 0.0005 | 0.0028 | 0.001 | 0.0009 | 0.0019 | 0.002 | 0.0008 | 0.0022 | 0.0024 | 0.0007 | 0.0014 | 0.0014 | 0.0004 | 0.0011 | 0.0017 |
Bud Periphery | 0.0021 | 0.0013 | 0.0007 | 0.0004 | 0.0012 | 0.0009 | 0.0008 | 0.0016 | 0.0047 | 0.0003 | 0.0038 | 0.0011 | 0.0009 | 0.0013 | 0.0025 | 0.0014 | 0.0018 | 0.0011 |
Bud Site | 0.0065 | 0.004 | 0.0014 | 0.0006 | 0.0026 | 0.0007 | 0.0007 | 0.0017 | 0.0024 | 0.0006 | 0.002 | 0.0008 | 0.0009 | 0.0013 | 0.0018 | 0.0004 | 0.0016 | 0.0007 |
Cell Periphery | 0.0016 | 0.0019 | 0.0014 | 0.0003 | 0.0014 | 0.002 | 0.0013 | 0.0024 | 0.0018 | 0.0007 | 0.0023 | 0.0023 | 0.0014 | 0.0022 | 0.0015 | 0.002 | 0.001 | 0.0023 |
Cytoplasm | 0.0143 | 0.0079 | 0.0086 | 0.0014 | 0.0074 | 0.0076 | 0.0076 | 0.0104 | 0.0094 | 0.0024 | 0.005 | 0.004 | 0.008 | 0.0289 | 0.0343 | 0.0077 | 0.0355 | 0.0098 |
Cytoplasmic Foci | 0.0231 | 0.013 | 0.0135 | 0.0114 | 0.0085 | 0.0185 | 0.0126 | 0.0064 | 0.0149 | 0.0105 | 0.005 | 0.0099 | 0.0143 | 0.0134 | 0.0338 | 0.011 | 0.0144 | 0.0172 |
Eisosomes | 0.0003 | 0 | 0.0001 | 0.0002 | 0.0003 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0101 | 0.0034 | 0.0032 | 0.0005 | 0.0079 | 0.0014 | 0.0039 | 0.0042 | 0.0042 | 0.001 | 0.0042 | 0.0026 | 0.0027 | 0.0052 | 0.0061 | 0.0016 | 0.0026 | 0.0042 |
Endosome | 0.2426 | 0.1498 | 0.1968 | 0.2316 | 0.1771 | 0.1472 | 0.281 | 0.1456 | 0.2371 | 0.2738 | 0.1672 | 0.1671 | 0.3029 | 0.2223 | 0.2878 | 0.4086 | 0.3352 | 0.2519 |
Golgi | 0.0111 | 0.003 | 0.008 | 0.0192 | 0.0127 | 0.0215 | 0.0075 | 0.0033 | 0.005 | 0.0042 | 0.0084 | 0.0039 | 0.0092 | 0.0093 | 0.0078 | 0.0084 | 0.0044 | 0.0064 |
Lipid Particles | 0.0254 | 0.014 | 0.0128 | 0.0444 | 0.0263 | 0.0102 | 0.02 | 0.0052 | 0.0082 | 0.0094 | 0.0029 | 0.0106 | 0.0178 | 0.011 | 0.0168 | 0.0086 | 0.0038 | 0.0217 |
Mitochondria | 0.0169 | 0.004 | 0.0194 | 0.0052 | 0.0169 | 0.0274 | 0.0116 | 0.0122 | 0.0107 | 0.0021 | 0.0047 | 0.0064 | 0.0165 | 0.0084 | 0.0119 | 0.0024 | 0.0037 | 0.0091 |
None | 0.0094 | 0.0132 | 0.0239 | 0.0002 | 0.0214 | 0.0021 | 0.0088 | 0.0011 | 0.0016 | 0.0001 | 0.0007 | 0.0006 | 0.0261 | 0.035 | 0.0595 | 0.0011 | 0.0029 | 0.0135 |
Nuclear Periphery | 0.0267 | 0.0123 | 0.0087 | 0.0013 | 0.0399 | 0.002 | 0.0107 | 0.0085 | 0.0158 | 0.0017 | 0.011 | 0.0102 | 0.003 | 0.0057 | 0.0073 | 0.0016 | 0.003 | 0.0055 |
Nucleolus | 0.0036 | 0.0028 | 0.0026 | 0.0009 | 0.0022 | 0.0014 | 0.0041 | 0.0031 | 0.0048 | 0.0014 | 0.0059 | 0.0039 | 0.0019 | 0.0027 | 0.0044 | 0.0008 | 0.0015 | 0.0028 |
Nucleus | 0.0149 | 0.0048 | 0.0037 | 0.0011 | 0.0043 | 0.0017 | 0.008 | 0.0058 | 0.011 | 0.0019 | 0.0186 | 0.0078 | 0.0028 | 0.0038 | 0.0088 | 0.0016 | 0.0048 | 0.004 |
Peroxisomes | 0.0024 | 0.0005 | 0.0037 | 0.0431 | 0.0017 | 0.0115 | 0.0034 | 0.0004 | 0.0012 | 0.0019 | 0.0005 | 0.0007 | 0.0034 | 0.0018 | 0.0023 | 0.0007 | 0.0004 | 0.0016 |
Punctate Nuclear | 0.0033 | 0.0004 | 0.0022 | 0.0006 | 0.0025 | 0.0003 | 0.0021 | 0.0001 | 0.0003 | 0.0001 | 0.0067 | 0.0003 | 0.0003 | 0.0003 | 0.0007 | 0.0001 | 0.0003 | 0.0002 |
Vacuole | 0.4186 | 0.5971 | 0.5009 | 0.463 | 0.4763 | 0.5803 | 0.4926 | 0.6801 | 0.54 | 0.5775 | 0.6545 | 0.6281 | 0.4655 | 0.5442 | 0.4149 | 0.4643 | 0.5204 | 0.5319 |
Vacuole Periphery | 0.1417 | 0.1653 | 0.17 | 0.1258 | 0.0955 | 0.155 | 0.1194 | 0.1053 | 0.119 | 0.1092 | 0.0929 | 0.1368 | 0.1145 | 0.1012 | 0.0953 | 0.077 | 0.061 | 0.114 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 37.6772 | 32.0773 | 39.2745 | 36.6705 | 31.4037 | 30.4837 | 37.7037 | 30.6464 | 31.7087 | 32.3359 |
Translational Efficiency | 3.7921 | 4.3975 | 2.9805 | 3.33 | 3.2698 | 3.6392 | 2.5073 | 2.9952 | 2.3591 | 2.8405 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
2187 | 951 | 103 | 1758 | 1485 | 2523 | 1963 | 1272 | 3672 | 3474 | 2066 | 3030 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 731.75 | 812.57 | 853.05 | 804.94 | 679.20 | 801.53 | 795.24 | 829.97 | 710.50 | 804.55 | 798.12 | 815.45 |
Standard Deviation | 87.38 | 113.72 | 92.58 | 101.81 | 78.66 | 104.48 | 95.36 | 125.31 | 87.83 | 107.20 | 96.05 | 112.95 |
Intensity Change Log 2 | — | 0.151141 | 0.221279 | 0.137530 | — | 0.238920 | 0.227554 | 0.289223 | — | 0.194063 | 0.224303 | 0.212544 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000254 | 0.000196 | 0.000710 | 0.000305 | 0.000072 | 0.000214 | 0.000064 | 0.000341 | 0.000181 | 0.000209 | 0.000096 | 0.000320 |
Bud Neck | 0.013700 | 0.017290 | 0.014304 | 0.031405 | 0.021076 | 0.020020 | 0.041043 | 0.023850 | 0.016683 | 0.019273 | 0.039710 | 0.028233 |
Bud Site | 0.007412 | 0.015184 | 0.006780 | 0.036159 | 0.008633 | 0.021550 | 0.010349 | 0.024031 | 0.007906 | 0.019807 | 0.010171 | 0.031067 |
Cell Periphery | 0.000340 | 0.000434 | 0.000153 | 0.000070 | 0.000241 | 0.000414 | 0.000077 | 0.000419 | 0.000300 | 0.000419 | 0.000081 | 0.000216 |
Cytoplasm | 0.043998 | 0.060806 | 0.139370* | 0.158690* | 0.115173* | 0.052651 | 0.131920* | 0.289123* | 0.072782 | 0.054883 | 0.132291* | 0.213446* |
Cytoplasmic Foci | 0.016752 | 0.031315 | 0.050961 | 0.048089 | 0.047271 | 0.019700 | 0.029581 | 0.060929 | 0.029094 | 0.022880 | 0.030647 | 0.053479 |
Eisosomes | 0.000021 | 0.000029 | 0.000099 | 0.000023 | 0.000049 | 0.000018 | 0.000028 | 0.000117 | 0.000032 | 0.000021 | 0.000032 | 0.000063 |
Endoplasmic Reticulum | 0.002115 | 0.003254 | 0.008906 | 0.001199 | 0.001739 | 0.002361 | 0.002668 | 0.001194 | 0.001963 | 0.002605 | 0.002979 | 0.001197 |
Endosome | 0.049662 | 0.062342 | 0.163225 | 0.142303 | 0.074373 | 0.064195 | 0.121150 | 0.049331 | 0.059655 | 0.063687 | 0.123248 | 0.103273 |
Golgi | 0.001505 | 0.002131 | 0.000314 | 0.002918 | 0.000558 | 0.002359 | 0.001507 | 0.001186 | 0.001122 | 0.002296 | 0.001448 | 0.002191 |
Lipid Particles | 0.001746 | 0.002515 | 0.007918 | 0.002223 | 0.002832 | 0.001130 | 0.002706 | 0.006137 | 0.002186 | 0.001509 | 0.002965 | 0.003866 |
Mitochondria | 0.002185 | 0.001265 | 0.000561 | 0.003388 | 0.001540 | 0.005072 | 0.002122 | 0.001584 | 0.001924 | 0.004030 | 0.002044 | 0.002630 |
Mitotic Spindle | 0.000532 | 0.000408 | 0.013661 | 0.033520 | 0.000038 | 0.003176 | 0.003895 | 0.015248 | 0.000332 | 0.002418 | 0.004382 | 0.025849 |
None | 0.000302 | 0.000439 | 0.001171 | 0.001021 | 0.000322 | 0.000885 | 0.001816 | 0.008433 | 0.000310 | 0.000763 | 0.001783 | 0.004133 |
Nuclear Periphery | 0.000177 | 0.000259 | 0.003748 | 0.001290 | 0.000311 | 0.000187 | 0.002551 | 0.001014 | 0.000231 | 0.000207 | 0.002611 | 0.001174 |
Nuclear Periphery Foci | 0.000093 | 0.000204 | 0.012806 | 0.001595 | 0.000182 | 0.000089 | 0.001558 | 0.001619 | 0.000129 | 0.000120 | 0.002118 | 0.001605 |
Nucleolus | 0.000594 | 0.000588 | 0.005166 | 0.002598 | 0.000918 | 0.000919 | 0.008810 | 0.004372 | 0.000725 | 0.000829 | 0.008629 | 0.003343 |
Nucleus | 0.008514 | 0.012272 | 0.255356* | 0.032893 | 0.020818 | 0.010395 | 0.096321 | 0.203383* | 0.013490 | 0.010909 | 0.104250 | 0.104465 |
Peroxisomes | 0.001206 | 0.001648 | 0.007201 | 0.006774 | 0.001568 | 0.001175 | 0.002489 | 0.008287 | 0.001353 | 0.001304 | 0.002724 | 0.007409 |
Vacuole | 0.836324* | 0.766532* | 0.305010* | 0.486216* | 0.693309* | 0.771500* | 0.531309* | 0.298014* | 0.778487* | 0.770140* | 0.520026* | 0.407208* |
Vacuole Periphery | 0.012566 | 0.020890 | 0.002581 | 0.007321 | 0.008977 | 0.021991 | 0.008036 | 0.001389 | 0.011115 | 0.021689 | 0.007764 | 0.004831 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 0.26 | -1.79 | -0.05 | -0.72 | 2.04 | -1.17 | -0.09 | -2.66 | -0.63 | -2.73 | -0.19 | 0.47 | -0.77 | -0.73 | -3.07 |
Bud Neck | -1.76 | 0.44 | -9.65 | -6.03 | -5.77 | 0.48 | -8.38 | 0.31 | -0.12 | 8.49 | -2.00 | -11.43 | -7.12 | -4.97 | 6.25 |
Bud Site | -4.38 | -0.56 | -9.26 | -5.13 | -7.57 | -7.11 | -1.10 | -4.78 | 0.73 | -4.13 | -8.72 | -1.88 | -10.28 | -2.56 | -8.33 |
Cell Periphery | -1.62 | 3.89 | 9.14 | 7.71 | 4.52 | -3.56 | 7.85 | -0.45 | 2.18 | -3.77 | -2.99 | 10.86 | 5.19 | 6.69 | -3.09 |
Cytoplasm | -3.41 | -5.11 | -23.75 | -17.87 | -2.96 | 11.30 | -4.84 | -19.35 | -29.99 | -15.85 | 5.10 | -14.38 | -30.20 | -34.40 | -13.03 |
Cytoplasmic Foci | -5.13 | -4.52 | -12.43 | -3.97 | 0.77 | 10.04 | 5.61 | -1.65 | -11.51 | -7.30 | 3.60 | -1.83 | -9.47 | -13.08 | -7.56 |
Eisosomes | -2.83 | -8.44 | -1.54 | 1.81 | 8.19 | 7.54 | 4.84 | -6.47 | -15.40 | -12.88 | 5.41 | -0.41 | -6.57 | -12.93 | -7.11 |
Endoplasmic Reticulum | -4.05 | -5.39 | 5.89 | 7.60 | 5.90 | -2.69 | -2.67 | 0.97 | 5.13 | 4.06 | -3.99 | -3.87 | 4.88 | 9.20 | 6.53 |
Endosome | -3.04 | -2.73 | -11.62 | -7.71 | 0.15 | 2.65 | -7.35 | 9.17 | 7.66 | 15.23 | -1.55 | -11.84 | -5.07 | -3.74 | 7.15 |
Golgi | -0.69 | 3.25 | -0.99 | 0.14 | -4.79 | -5.37 | -1.79 | -1.03 | 4.00 | 1.11 | -2.99 | -0.29 | -1.19 | 2.04 | -0.53 |
Lipid Particles | -0.93 | -5.29 | -1.70 | -0.02 | 4.96 | 5.94 | 0.19 | -2.23 | -4.73 | -2.40 | 1.95 | -2.73 | -2.82 | -5.00 | -0.77 |
Mitochondria | 1.74 | 3.29 | -1.14 | -3.64 | -6.05 | -4.23 | -1.01 | 0.71 | 5.40 | 2.49 | -3.49 | -0.26 | -0.51 | 3.44 | -0.28 |
Mitotic Spindle | 0.41 | -1.94 | -10.88 | -10.98 | -1.41 | -4.44 | -7.24 | -6.86 | -5.45 | -4.44 | -3.76 | -6.69 | -12.83 | -11.51 | -9.06 |
None | -0.59 | -3.44 | -2.89 | -1.89 | 0.55 | -2.78 | -4.19 | -3.92 | -3.48 | -2.81 | -2.84 | -4.42 | -4.51 | -3.64 | -1.89 |
Nuclear Periphery | -1.65 | -5.93 | -11.17 | -10.43 | 3.25 | 2.60 | -11.03 | -11.10 | -12.70 | 5.13 | 0.78 | -12.21 | -15.10 | -15.41 | 4.79 |
Nuclear Periphery Foci | -2.60 | -3.39 | -6.89 | -6.45 | 3.08 | 4.45 | -4.59 | -7.73 | -8.09 | -0.08 | 0.28 | -5.73 | -10.15 | -10.23 | 1.89 |
Nucleolus | 0.03 | -3.45 | -11.06 | -12.26 | 1.19 | 0.04 | -11.52 | -4.08 | -4.57 | 6.95 | -0.62 | -12.69 | -9.15 | -9.72 | 8.25 |
Nucleus | -2.22 | -8.62 | -15.98 | -12.36 | 7.28 | 6.04 | -20.34 | -22.23 | -24.29 | -8.86 | 2.22 | -24.46 | -25.60 | -26.44 | 2.48 |
Peroxisomes | -1.33 | -3.34 | -7.62 | -7.16 | 0.03 | 1.59 | -3.29 | -4.72 | -5.15 | -3.78 | 0.25 | -4.86 | -8.27 | -8.64 | -5.67 |
Vacuole | 7.02 | 17.35 | 39.40* | 24.20 | -4.85 | -8.32 | 17.70 | 28.10 | 39.01* | 13.10 | 1.34 | 34.48 | 47.23* | 44.78* | 7.60 |
Vacuole Periphery | -5.59 | 11.91 | 10.17 | 11.09 | -4.42 | -11.93 | 2.54 | 12.25 | 23.38 | 13.54* | -12.22 | 7.27 | 15.20 | 23.30 | 6.94 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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