ORF
Human Ortholog
Description Putative X-Pro aminopeptidase; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YFR006W is not an essential gene
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.97 0.98 1.0 0.98 0.98 0.99 0.97 0.97 0.97 0.94 0.96 0.96 0.99 1.0 0.98 0.98 0.95 0.96 0.89 0.91 0.91 0.9 0.89 0.8
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0.05 0.08 0.08 0.08 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.05 0 0 0 0 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 1 0 0 2 1 0 2 2
Bud 3 1 5 3 4 4 10 14 13 9 4 2 1 0 0 0 0 0 1 0 1 0 0 2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 3 6 5 4 11 8 2 3 0 1 0 6 7 9 0 0 0 0 0 0
Cytoplasm 346 292 282 176 382 435 626 607 737 443 165 231 127 162 148 412 257 205 321 276 260 66 182 132
Endoplasmic Reticulum 0 1 0 1 2 1 0 0 1 2 0 1 0 0 1 21 9 10 1 0 0 0 1 5
Endosome 1 0 0 0 1 0 0 0 0 0 0 0 0 0 0 1 0 1 7 3 6 0 2 3
Golgi 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 3 1 0 0 0 1 2
Mitochondria 4 2 0 0 2 5 12 14 36 38 13 20 0 0 1 2 1 2 0 4 0 0 1 1
Nucleus 2 1 0 1 0 0 3 1 2 3 1 1 0 0 0 1 1 1 4 2 1 0 1 0
Nuclear Periphery 0 0 0 0 0 0 0 1 0 0 0 0 0 0 1 0 0 1 1 0 0 0 0 1
Nucleolus 0 0 0 0 0 0 0 0 1 0 0 0 1 0 0 0 0 0 0 0 0 1 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0
Vac/Vac Membrane 4 1 1 2 7 1 14 14 4 11 1 1 2 0 0 9 13 3 19 8 7 1 5 8
Unique Cell Count 355 297 283 179 389 438 643 627 762 469 172 241 128 162 151 421 270 214 361 303 287 74 204 166
Labelled Cell Count 360 299 288 183 401 452 670 655 805 514 186 260 131 163 151 452 291 235 361 303 287 74 204 166


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.5 8.1 7.2 6.9 7.4 6.5 5.6 6.1 6.0 5.4 5.9 5.6 8.0 7.2 7.0 9.1 10.5 11.5 8.2 7.4 8.0
Std Deviation (1e-4) 1.1 1.4 1.2 1.0 1.6 1.4 1.1 1.1 0.9 1.1 1.3 1.2 2.3 1.4 1.0 1.8 1.6 2.2 2.5 1.4 1.5
Intensity Change (Log2) – – – -0.06 0.03 -0.15 -0.36 -0.24 -0.27 -0.42 -0.3 -0.37 0.15 -0.01 -0.04 0.34 0.54 0.67 0.17 0.04 0.14


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0.5 0 0.1 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -1.5 -1.7 -0.6 -2.3 -2.6 -2.7 -3.7 -2.9 -3.0 -0.6 0.8 -1.7 -2.0 -3.3 -3.0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 3.8 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 3.7 4.9 0 4.9 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 3.3 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.0674 5.3327 4.1436 4.9121 3.8606 4.9485 4.2805 6.7566 5.7008 5.681 5.0743 6.0131 6.3548 7.3472 6.6318 6.5229 6.3177 6.8964
Actin 0.0742 0.0004 0.0153 0.0003 0.0003 0.0099 0.0121 0.0012 0.0039 0.0054 0.0122 0.0044 0.0391 0.0006 0.0019 0.0452 0.0008 0.0013
Bud 0.0005 0.0001 0.0001 0.0001 0.0001 0.0021 0.0014 0.0051 0.0003 0.001 0.0002 0.0003 0.0012 0.0002 0.0004 0.0005 0.0003 0.0001
Bud Neck 0.0087 0.0002 0.0003 0.0004 0.0005 0.0008 0.0018 0.0004 0.0005 0.0028 0.0006 0.0007 0.0359 0.0002 0.0007 0.0008 0.0005 0.0007
Bud Periphery 0.0006 0 0.0001 0 0 0.0011 0.0017 0.0024 0.0001 0.0017 0.0001 0.0002 0.0011 0.0001 0.0004 0.0006 0.0001 0.0001
Bud Site 0.0043 0.0003 0.0005 0.0001 0.0001 0.0004 0.0174 0.0175 0.0007 0.0032 0.0003 0.0001 0.0263 0.0008 0.001 0.0011 0.0001 0.0001
Cell Periphery 0.0004 0.0001 0.0002 0 0 0 0.0003 0.0003 0.0001 0.0009 0 0 0.0004 0 0.0001 0.0001 0 0
Cytoplasm 0.7401 0.9778 0.8849 0.9815 0.8968 0.9553 0.6817 0.8516 0.8513 0.8585 0.8081 0.9117 0.7628 0.8646 0.8947 0.8152 0.9444 0.9614
Cytoplasmic Foci 0.0164 0.0009 0.0053 0.0013 0.0026 0.005 0.0598 0.0051 0.0103 0.0123 0.0464 0.0073 0.0272 0.0413 0.013 0.0163 0.006 0.0038
Eisosomes 0.0006 0 0.0001 0 0 0 0.0001 0.0001 0 0 0.0001 0 0.0002 0 0 0.0002 0 0
Endoplasmic Reticulum 0.0066 0.0026 0.0033 0.0013 0.0017 0.0008 0.0019 0.003 0.0042 0.0034 0.0011 0.0021 0.0067 0.0022 0.003 0.0019 0.0021 0.0019
Endosome 0.0574 0.0004 0.0025 0.0007 0.0006 0.0009 0.0359 0.0034 0.0078 0.0071 0.0117 0.0118 0.0212 0.0209 0.0231 0.0076 0.0037 0.0042
Golgi 0.0161 0 0.0048 0 0 0.0009 0.0127 0.0003 0.0024 0.0008 0.0048 0.0055 0.0093 0.0042 0.0113 0.0146 0.0002 0.0015
Lipid Particles 0.0114 0 0.0079 0 0 0 0.0575 0.0003 0.0034 0.001 0.022 0.0003 0.0055 0.0011 0.0081 0.013 0 0.0003
Mitochondria 0.006 0.0001 0.0006 0.0001 0.0001 0.0087 0.0157 0.0182 0.0007 0.0004 0.0009 0.0009 0.003 0.0013 0.0048 0.0078 0.0002 0.0021
None 0.0255 0.0103 0.0677 0.0027 0.093 0.0041 0.024 0.081 0.1028 0.0934 0.0309 0.0498 0.0383 0.0487 0.0174 0.0387 0.036 0.0141
Nuclear Periphery 0.0062 0.0024 0.0011 0.0015 0.0009 0.0006 0.0019 0.0013 0.0011 0.001 0.0018 0.001 0.0054 0.0009 0.0037 0.0007 0.0011 0.0009
Nucleolus 0.0002 0 0 0 0 0 0.0003 0.0002 0.0002 0.0001 0.0002 0 0.0007 0 0.0002 0.0001 0 0
Nucleus 0.0032 0.0034 0.002 0.0087 0.0029 0.0044 0.0024 0.0035 0.0018 0.0022 0.0026 0.0022 0.0032 0.0021 0.0066 0.0066 0.003 0.0062
Peroxisomes 0.0138 0 0.0021 0 0 0.0041 0.0625 0.0002 0.0064 0.0006 0.0319 0.0003 0.0054 0.0095 0.0048 0.0275 0.0001 0.0001
Punctate Nuclear 0.001 0.0002 0.0004 0.0004 0.0002 0.0002 0.0067 0.0006 0.001 0.001 0.0235 0.0006 0.0027 0.0006 0.0016 0.0009 0.0005 0.0006
Vacuole 0.0055 0.0007 0.0007 0.0006 0.0003 0.0004 0.0014 0.0019 0.0008 0.003 0.0004 0.0005 0.0038 0.0005 0.0022 0.0005 0.0007 0.0005
Vacuole Periphery 0.0014 0.0001 0.0001 0.0001 0 0.0001 0.0007 0.0025 0.0001 0.0003 0.0002 0.0002 0.0006 0.0002 0.0009 0.0003 0.0002 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 73.7662 66.8626 55.9875 80.02 60.4985 63.781 76.4429 71.1639 80.5215 69.8623
Translational Efficiency 1.4805 1.267 1.5085 1.1645 1.3219 1.6027 1.2707 1.1883 1.0934 1.196

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1970 1002 2127 1974 1471 1437 105 2036 3441 2439 2232 4010

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 754.29 861.26 1063.02 1054.82 743.21 827.03 1041.46 1024.71 749.55 841.09 1062.01 1039.53
Standard Deviation 92.58 142.89 136.31 136.59 101.44 134.36 134.57 143.55 96.63 138.95 136.31 140.97
Intensity Change Log 2 — 0.191330 0.494978 0.483806 — 0.154170 0.486766 0.463374 — 0.173007 0.490908 0.473701

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000065 0.000173 0.000119 0.000596 0.000118 0.000485 0.000283 0.000453 0.000088 0.000357 0.000127 0.000523
Bud Neck 0.008024 0.004326 0.001957 0.005189 0.004171 0.006563 0.000745 0.008127 0.006377 0.005644 0.001900 0.006681
Bud Site 0.005857 0.011841 0.005213 0.028877 0.004128 0.009774 0.001930 0.023744 0.005118 0.010623 0.005059 0.026271
Cell Periphery 0.000078 0.000117 0.000041 0.000067 0.000065 0.000042 0.000127 0.000156 0.000073 0.000073 0.000045 0.000112
Cytoplasm 0.649131* 0.642185* 0.593199* 0.527943* 0.627458* 0.622438* 0.471483* 0.537062* 0.639866* 0.630551* 0.587473* 0.532573*
Cytoplasmic Foci 0.125022 0.101299 0.003720 0.019042 0.108798 0.102283 0.018216 0.022058 0.118086 0.101879 0.004402 0.020573
Eisosomes 0.000051 0.000031 0.000008 0.000016 0.000042 0.000037 0.000069 0.000029 0.000047 0.000035 0.000011 0.000023
Endoplasmic Reticulum 0.000510 0.000702 0.001113 0.001340 0.001003 0.000391 0.003238 0.000853 0.000720 0.000519 0.001213 0.001093
Endosome 0.011473 0.020743 0.000974 0.007976 0.016172 0.022119 0.003590 0.012523 0.013482 0.021553 0.001097 0.010285
Golgi 0.001014 0.002604 0.000054 0.012121 0.000870 0.001917 0.000099 0.007851 0.000953 0.002199 0.000056 0.009953
Lipid Particles 0.002082 0.002466 0.000413 0.000924 0.002105 0.001527 0.002071 0.000681 0.002092 0.001913 0.000491 0.000801
Mitochondria 0.001848 0.004093 0.000402 0.001306 0.001095 0.002483 0.000180 0.003718 0.001526 0.003144 0.000392 0.002530
Mitotic Spindle 0.000720 0.003183 0.007112 0.128446* 0.002323 0.004418 0.000616 0.056144 0.001406 0.003910 0.006806 0.091736
None 0.005083 0.002137 0.006884 0.005101 0.002788 0.002141 0.003324 0.007510 0.004102 0.002139 0.006717 0.006324
Nuclear Periphery 0.000177 0.000272 0.001506 0.001546 0.000320 0.000316 0.001061 0.000892 0.000238 0.000298 0.001485 0.001214
Nuclear Periphery Foci 0.000667 0.002424 0.001281 0.002624 0.001364 0.000814 0.003324 0.001063 0.000965 0.001475 0.001377 0.001831
Nucleolus 0.000906 0.002892 0.000178 0.000447 0.001175 0.001037 0.000240 0.001276 0.001021 0.001799 0.000181 0.000868
Nucleus 0.068530 0.033135 0.337013* 0.102692 0.068749 0.029404 0.272018* 0.116315 0.068624 0.030937 0.333956* 0.109609
Peroxisomes 0.001116 0.002296 0.000064 0.000838 0.001526 0.002957 0.000294 0.001085 0.001291 0.002685 0.000075 0.000963
Vacuole 0.116811 0.160996 0.038655 0.147646 0.154917 0.187380 0.216917* 0.193744 0.133101 0.176541 0.047041 0.171051
Vacuole Periphery 0.000835 0.002087 0.000093 0.005263 0.000812 0.001472 0.000174 0.004719 0.000825 0.001725 0.000097 0.004987

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -4.89 -5.79 -5.23 -2.71 -2.58 -1.50 -3.71 -3.79 0.81 2.23 -1.95 -5.09 -6.72 0.28 -2.63
Bud Neck 4.55 6.93 1.96 -3.95 -8.84 -3.32 6.35 -3.74 -0.35 -9.16 1.65 7.57 -0.22 -2.22 -10.41
Bud Site -4.62 1.15 -4.24 -0.05 -5.02 -5.56 1.28 -7.35 -2.83 -7.79 -6.48 0.70 -7.77 -2.17 -7.05
Cell Periphery -0.80 6.64 5.62 1.34 -2.13 3.57 -4.07 -1.48 -3.42 2.65 0.30 4.45 0.24 -0.20 -2.27
Cytoplasm 0.92 19.54 27.24 19.64 9.08 0.35 9.10 13.90 13.01 -4.26 1.45 21.20 28.75 23.15 5.02
Cytoplasmic Foci 4.80 36.42 32.55 20.92 -12.23 1.51 23.92 26.64 24.90 -1.40 4.93 47.26 41.92 32.61 -16.17
Eisosomes 5.58 19.63 14.76 1.94 -9.91 0.70 -4.11 9.21 1.09 6.32 2.17 19.98 16.94 1.46 -5.34
Endoplasmic Reticulum -2.29 -14.88 -9.37 -5.91 6.79 2.01 -4.10 1.05 -5.17 4.72 1.57 -8.53 -1.64 -8.40 11.43
Endosome -4.63 17.38 12.66 10.15 -7.49 -2.55 12.01 9.37 10.22 -3.15 -5.53 22.11 14.51 14.55 -9.41
Golgi -1.87 4.31 -1.14 0.63 -2.76 -1.42 2.82 -2.25 -0.67 -3.84 -2.24 5.24 -2.34 -0.05 -4.68
Lipid Particles -0.37 5.98 1.28 1.14 -12.52 2.08 -0.33 6.32 1.56 3.75 1.08 8.63 4.34 1.62 -10.83
Mitochondria -1.31 2.76 2.16 2.27 -5.67 -1.25 1.81 -0.56 0.90 -2.97 -1.63 2.97 1.07 2.34 -2.97
Mitotic Spindle -2.19 -5.58 -16.96 -16.17 -15.12 -1.26 -0.54 -13.67 -13.04 -10.33 -2.56 -4.96 -21.81 -20.66 -18.87
None 4.10 -1.98 -3.63 -10.57 -3.13 0.97 -0.13 -5.08 -4.78 -4.17 3.66 -4.98 -5.46 -9.19 -1.79
Nuclear Periphery -1.64 -24.64 -26.83 -23.67 -5.30 0.20 -8.43 -17.28 -13.01 -0.94 -1.04 -24.58 -31.60 -25.63 -0.14
Nuclear Periphery Foci -8.09 -7.32 -10.43 -3.69 -5.20 3.75 -4.09 -0.25 -3.35 4.01 -4.78 -6.43 -9.45 -5.55 -1.95
Nucleolus -1.59 4.29 -2.92 0.85 -6.67 0.55 2.92 -0.52 -1.42 -6.45 -1.24 4.60 -2.28 0.19 -9.19
Nucleus 12.81 -59.01 -21.38 -29.37 37.71 12.56 -9.04 -12.92 -26.88 6.54 19.09 -59.94 -24.28 -40.20 44.06
Peroxisomes -3.16 7.70 3.39 5.03 -3.94 -2.87 5.94 4.26 5.67 -3.91 -4.34 9.95 5.49 7.42 -6.55
Vacuole -7.38 7.21 -23.62 -13.17 -28.61 -4.74 -5.39 -14.20 -9.38 1.59 -9.84 9.26 -27.23 -15.62 -32.63
Vacuole Periphery -3.01 9.05 -0.01 2.38 -2.65 -2.21 3.44 -1.49 -0.17 -3.48 -3.86 7.76 -1.21 1.55 -4.46
ORF
Human Ortholog
Description Putative X-Pro aminopeptidase; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YFR006W is not an essential gene
Localization
Cell Percentages cytoplasm (67%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

YFR006W

YFR006W


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Yfr006w-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available