Standard name
Human Ortholog
Description Subunit of the Isw1a complex; Isw1a has nucleosome-stimulated ATPase activity and represses transcription initiation by specific positioning of a promoter proximal dinucleosome; promotes nucleosome shifts in the 5 prime direction; IOC3 has a paralog, ESC8, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0.07 – 0.05 0.06 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.05 0 0.05 0 0 0 0 0 0 – 0 0 0 0.07 0.12 0.05 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.05 0 0 0 0 0.1 0.11 0.05 0.26 – 0.26 0.14 0 0 0 0 0 0.07 0 0 0 0 0 0
Nucleus 0.95 0.93 0.93 0.94 0.93 0.9 0.88 0.85 0.79 – 0.8 0.82 0.92 0.89 0.78 0.86 0.91 0.85 0.84 0.93 0.89 0.76 0.71 0.61
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.3 0.23 0 0.05 0.07 0.11 0.08 0.14 0.23 – 0.18 0.18 0.13 0.12 0.16 0.07 0.06 0.07 0.08 0 0 0.13 0.15 0.16
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.05 0 0 0 0.07 0.07 0.15
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 1 0 0 0 0 0 0 0 0 1
Bud 0 0 0 1 0 2 3 3 3 – 1 3 1 0 0 3 0 0 1 0 4 1 5 4
Bud Neck 0 0 0 0 1 0 0 2 1 – 3 4 0 0 0 0 0 0 0 0 0 0 1 1
Bud Site 0 0 2 0 3 11 18 5 26 – 12 19 3 0 2 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 1 0 0 0 0 1 0 0 0 0 0 0 0
Cytoplasm 1 2 16 7 11 5 16 12 12 – 3 7 18 42 74 44 14 23 0 0 1 0 2 1
Endoplasmic Reticulum 0 0 0 0 0 0 0 1 0 – 0 0 0 0 0 10 6 6 0 0 1 1 2 2
Endosome 0 0 0 1 1 0 0 2 0 – 0 0 0 5 4 6 4 3 0 0 2 0 4 7
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 2 2 3
Mitochondria 1 5 2 3 1 38 63 26 91 – 59 48 5 2 1 20 11 36 0 0 3 0 3 9
Nucleus 19 108 279 179 370 356 486 435 275 – 179 272 403 499 477 729 428 444 48 114 284 151 294 350
Nuclear Periphery 0 0 1 0 1 5 9 12 5 – 3 5 0 2 1 11 3 2 0 0 0 0 1 2
Nucleolus 6 27 11 10 26 45 47 71 82 – 41 59 57 66 96 61 29 35 4 4 8 26 63 92
Peroxisomes 0 1 0 0 0 0 0 1 0 – 0 0 2 3 5 2 1 1 0 0 0 0 1 0
SpindlePole 0 0 5 2 4 3 5 13 2 – 2 4 2 4 8 6 5 5 0 0 0 1 0 5
Vac/Vac Membrane 0 0 1 0 4 1 3 6 5 – 1 1 3 0 5 34 9 24 1 1 8 13 31 82
Unique Cell Count 20 116 301 191 396 396 555 510 350 225 333 440 562 611 849 468 520 58 124 320 201 418 570
Labelled Cell Count 27 143 317 203 422 466 650 589 502 304 423 494 623 674 926 511 579 58 124 320 201 418 570


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.1 7.4 7.4 6.7 7.4 5.8 6.0 6.4 5.1 – 5.1 5.4 8.0 8.1 7.8 10.1 9.8 9.8 7.2 7.5 7.8
Std Deviation (1e-4) 1.3 1.8 1.4 1.3 1.5 1.3 1.3 1.3 1.3 – 1.9 1.3 1.9 2.4 2.5 2.9 3.1 2.8 1.5 2.0 2.1
Intensity Change (Log2) – – – -0.15 0.0 -0.36 -0.3 -0.22 -0.54 – -0.55 -0.46 0.11 0.13 0.08 0.44 0.41 0.4 -0.04 0.01 0.07


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 2.0 2.4 0 4.2 – 3.3 3.5 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cytoplasm -0.8 -1.7 -3.1 -1.8 -2.2 -1.2 – -2.4 -2.2 -0.8 1.2 3.2 -0.1 -1.6 -0.6
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 0 0 5.0 5.6 3.3 9.2 – 9.1 6.4 0 0 0 1.8 1.8 4.1
Nucleus 0.4 0.4 -1.3 -2.3 -3.1 -5.0 – -4.4 -4.1 -0.5 -1.8 -5.5 -3.1 -0.6 -3.1
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0.8 1.7 3.7 2.7 4.7 7.2 – 5.5 5.6 4.3 4.0 5.3 2.2 1.5 1.8
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0.8 0 – 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 – 0 0 0 0 0 3.2 0 3.4

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 5.0363 4.7358 4.4777 3.8077 3.6025 4.3769 3.672 3.0459 3.2313 2.8268 2.4291 2.9493 5.2275 4.1412 3.8698 4.1621 4.1235 3.5406
Actin 0.0494 0.0006 0.0167 0.001 0.0001 0 0.0357 0 0.0169 0 0.034 0.0016 0.0729 0 0.0046 0 0.0064 0.011
Bud 0.0007 0.0002 0.0003 0 0.0001 0 0.0007 0 0.0002 0 0.0018 0 0.0005 0 0.0002 0 0.0004 0
Bud Neck 0.0042 0.0002 0.0007 0.0001 0.0009 0.0014 0.0005 0 0.0004 0 0.0011 0.0006 0.0014 0.0001 0.0002 0 0.0005 0.0003
Bud Periphery 0.0011 0.0002 0.0005 0.0001 0.0003 0.0001 0.0008 0 0.0003 0 0.0034 0.0001 0.0007 0 0.0003 0 0.001 0
Bud Site 0.0272 0.0006 0.0105 0.0001 0.0002 0.0001 0.0028 0 0.002 0 0.0027 0.0001 0.0018 0 0.0006 0 0.0006 0
Cell Periphery 0.0008 0 0.0005 0.0001 0.0001 0.0001 0.0002 0 0.0001 0 0.0009 0 0.0003 0 0.0001 0 0.0003 0
Cytoplasm 0.0036 0.0006 0.0004 0 0.0006 0 0.0058 0 0.0007 0 0.0006 0.0004 0.0027 0 0.0013 0 0.0014 0
Cytoplasmic Foci 0.0086 0.0011 0.001 0.0006 0.0014 0 0.0052 0 0.0051 0 0.0032 0.0062 0.0068 0 0.0021 0 0.0109 0
Eisosomes 0.0007 0 0.0004 0.0001 0 0 0.0002 0 0.0005 0 0.0001 0 0.0013 0 0 0 0.0001 0
Endoplasmic Reticulum 0.005 0.0006 0.0012 0.0001 0.0042 0 0.0025 0 0.0003 0 0.0009 0.0004 0.0025 0 0.0005 0 0.0008 0
Endosome 0.0129 0.0122 0.0052 0.0031 0.0692 0 0.0209 0 0.0037 0 0.006 0.0097 0.0058 0 0.0021 0 0.0099 0
Golgi 0.0067 0.0036 0.0074 0.0027 0.0074 0 0.0072 0 0.0023 0 0.0079 0.0019 0.0049 0 0.0028 0 0.0093 0.0002
Lipid Particles 0.0247 0.0004 0.0007 0.0081 0.0059 0 0.0097 0 0.006 0 0.0024 0.0079 0.0048 0 0.0009 0 0.0252 0.0001
Mitochondria 0.0035 0.0027 0.0333 0.0798 0.0033 0.0001 0.0093 0.0001 0.0193 0 0.0115 0.0009 0.0048 0.0001 0.0035 0 0.008 0.0001
None 0.0034 0.0001 0.0002 0.0001 0.0001 0 0.0026 0 0.0003 0 0.0004 0.0002 0.0122 0 0.0004 0 0.0019 0
Nuclear Periphery 0.0106 0.0012 0.0004 0.0003 0.0036 0.0001 0.01 0.0002 0.0025 0.0002 0.0024 0.0126 0.0042 0.0002 0.0022 0.0001 0.0057 0
Nucleolus 0.0328 0.0318 0.0153 0.0115 0.1184 0.1297 0.0233 0.0181 0.0132 0.0121 0.1238 0.0586 0.0146 0.0267 0.0159 0.0112 0.1944 0.0568
Nucleus 0.771 0.9418 0.9036 0.8887 0.767 0.8677 0.8522 0.9813 0.9123 0.9875 0.7883 0.8922 0.8395 0.9722 0.9571 0.9885 0.6561 0.9309
Peroxisomes 0.0109 0.0001 0.0008 0.0013 0.0001 0 0.0014 0 0.002 0 0.0015 0.0009 0.0095 0 0.0009 0 0.006 0.0001
Punctate Nuclear 0.0185 0.0007 0.0001 0.0001 0.0008 0.0003 0.0041 0.0003 0.0104 0.0001 0.0012 0.0041 0.0076 0.0005 0.003 0.0001 0.0566 0.0001
Vacuole 0.003 0.0009 0.0004 0.0007 0.0091 0.0001 0.0027 0 0.0007 0 0.0037 0.0006 0.0006 0 0.0007 0 0.002 0
Vacuole Periphery 0.001 0.0005 0.0003 0.0013 0.0072 0 0.0021 0 0.0007 0 0.0022 0.0012 0.0005 0 0.0004 0 0.0026 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 31.5058 29.6247 20.4347 28.431 24.2066 33.1211 27.1058 22.3521 25.7877 31.912
Translational Efficiency 0.8998 0.7362 0.9193 0.7096 0.8107 0.786 0.7554 0.8688 0.7281 0.7106

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1575 680 1028 960 669 769 1067 2172 2244 1449 2095 3132

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 777.19 943.73 1153.31 1061.74 691.14 964.74 1084.21 1329.82 751.54 954.88 1118.12 1247.65
Standard Deviation 106.11 163.14 197.64 218.61 119.91 171.80 165.91 279.46 117.21 168.12 185.42 289.97
Intensity Change Log 2 — 0.280107 0.569441 0.450091 — 0.481162 0.649594 0.944181 — 0.378240 0.607724 0.703776

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000278 0.000597 0.001075 0.000906 0.000663 0.001033 0.000765 0.000317 0.000393 0.000828 0.000917 0.000497
Bud Neck 0.004029 0.012651 0.027640 0.018805 0.004286 0.015138 0.014662 0.025742 0.004105 0.013971 0.021030 0.023616
Bud Site 0.000547 0.001061 0.001935 0.002783 0.001789 0.002132 0.001029 0.001353 0.000917 0.001629 0.001474 0.001792
Cell Periphery 0.000095 0.000077 0.000058 0.000131 0.000322 0.000121 0.000046 0.000120 0.000163 0.000100 0.000052 0.000124
Cytoplasm 0.001511 0.001729 0.000667 0.002101 0.003144 0.001739 0.000310 0.000690 0.001998 0.001735 0.000485 0.001123
Cytoplasmic Foci 0.000240 0.000148 0.000079 0.000361 0.001563 0.001658 0.000114 0.000012 0.000635 0.000950 0.000097 0.000119
Eisosomes 0.000026 0.000048 0.000068 0.000040 0.000042 0.000050 0.000061 0.000060 0.000031 0.000049 0.000064 0.000054
Endoplasmic Reticulum 0.002200 0.002120 0.001619 0.001305 0.003536 0.004904 0.001760 0.001744 0.002598 0.003597 0.001690 0.001610
Endosome 0.000264 0.000245 0.000111 0.000112 0.000331 0.001157 0.000163 0.000079 0.000284 0.000729 0.000138 0.000089
Golgi 0.000145 0.000146 0.000120 0.000109 0.000192 0.000204 0.000105 0.000028 0.000159 0.000177 0.000112 0.000053
Lipid Particles 0.000118 0.000206 0.000046 0.000045 0.000219 0.000711 0.000929 0.000004 0.000149 0.000474 0.000496 0.000017
Mitochondria 0.001228 0.000990 0.002135 0.001681 0.001268 0.003054 0.001237 0.000812 0.001240 0.002085 0.001678 0.001078
Mitotic Spindle 0.003143 0.004900 0.003412 0.007265 0.005897 0.007527 0.003816 0.001017 0.003964 0.006294 0.003617 0.002932
None 0.005822 0.001275 0.002206 0.004991 0.020292 0.006630 0.003691 0.001113 0.010136 0.004117 0.002962 0.002302
Nuclear Periphery 0.000172 0.000162 0.000212 0.000269 0.000242 0.000589 0.000143 0.000292 0.000193 0.000389 0.000177 0.000285
Nuclear Periphery Foci 0.000199 0.000291 0.000074 0.000231 0.001222 0.000436 0.000120 0.000033 0.000504 0.000368 0.000097 0.000094
Nucleolus 0.045077 0.052861 0.040893 0.032943 0.101958 0.044254 0.046447 0.007012 0.062035 0.048293 0.043721 0.014960
Nucleus 0.934072* 0.918859* 0.915463* 0.924325* 0.851539* 0.902404* 0.923197* 0.958921* 0.909467* 0.910126* 0.919402* 0.948317*
Peroxisomes 0.000452 0.000751 0.001415 0.000810 0.001010 0.002208 0.000968 0.000041 0.000619 0.001524 0.001187 0.000277
Vacuole 0.000196 0.000398 0.000527 0.000616 0.000279 0.002665 0.000296 0.000356 0.000221 0.001601 0.000410 0.000435
Vacuole Periphery 0.000185 0.000484 0.000248 0.000173 0.000207 0.001387 0.000141 0.000254 0.000192 0.000963 0.000193 0.000229

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -4.77 -16.73 -7.66 -3.46 2.76 -3.03 -1.65 3.16 8.78 12.84 -7.27 -14.23 -3.04 4.99 11.35
Bud Neck -6.96 -17.99 -13.37 -5.10 5.12 -8.18 -10.33 -14.45 -6.16 -7.33 -11.22 -21.04 -19.47 -8.28 -1.76
Bud Site -2.58 -10.66 -4.42 -3.27 -0.71 -1.06 0.82 0.30 1.45 -0.86 -2.23 -4.37 -2.98 0.15 -0.31
Cell Periphery 0.45 2.16 -1.21 -1.19 -2.74 1.95 2.72 2.05 0.42 -3.90 1.67 3.39 1.32 -0.69 -4.55
Cytoplasm -0.37 1.58 -0.64 0.02 -2.32 1.21 2.34 2.13 2.67 -1.26 0.13 2.77 1.56 1.06 -2.53
Cytoplasmic Foci -0.37 1.22 -0.96 -0.48 -1.64 0.12 1.61 1.70 2.06 1.25 -0.61 1.87 1.58 1.94 -0.87
Eisosomes -7.34 -18.16 -8.22 2.18 11.38 -2.53 -7.01 -2.63 -1.02 1.20 -8.90 -20.45 -6.11 -0.48 4.50
Endoplasmic Reticulum 0.35 2.11 5.87 4.10 3.66 -3.70 4.96 6.16 10.75 1.91 -5.21 4.74 7.67 10.91 3.23
Endosome -0.18 1.47 1.41 1.57 -0.21 -2.45 2.22 4.87 3.28 1.38 -2.38 1.90 2.78 3.62 1.48
Golgi -0.68 -0.16 -0.11 0.77 0.17 0.36 1.96 3.59 4.25 5.38 -0.62 0.75 2.25 3.39 6.32
Lipid Particles -1.02 1.61 1.55 1.26 0.03 -1.04 -0.72 3.99 1.60 1.04 -1.52 -0.71 4.15 2.04 1.06
Mitochondria 0.42 -2.72 -1.29 -2.76 2.93 -2.54 -0.19 1.25 3.39 2.83 -1.73 -1.94 0.15 2.47 6.01
Mitotic Spindle -0.98 -0.31 -2.31 -1.18 -2.10 -0.46 0.49 1.93 2.70 1.93 -1.37 -0.08 0.08 1.49 0.17
None 4.65 3.23 0.74 -3.19 -2.15 3.58 4.62 5.48 3.00 1.91 3.96 5.25 6.09 1.74 0.56
Nuclear Periphery 0.12 -2.21 -3.57 -2.40 -1.68 -1.88 1.02 -0.64 1.50 -1.55 -1.97 -0.53 -1.96 0.74 -1.68
Nuclear Periphery Foci -0.85 1.81 -0.81 0.10 -1.74 2.08 3.02 3.25 2.78 1.42 0.78 3.36 2.99 2.29 -0.59
Nucleolus -2.30 -1.06 1.90 3.64 2.61 11.39 10.46 21.59 13.45 14.05 4.71 4.88 20.26 14.06 14.31
Nucleus 3.11 6.52 3.96 0.56 -1.59 -5.46 -8.62 -14.58 -8.97 -8.50 0.37 -0.33 -8.92 -7.85 -9.25
Peroxisomes -1.41 -6.48 -2.95 0.34 4.04 -1.66 0.48 8.63 3.22 11.66 -2.48 -5.52 3.12 3.30 11.65
Vacuole -1.66 -6.42 -2.76 -1.27 -0.22 -2.49 -1.48 -1.01 2.40 0.87 -2.71 -4.33 -3.19 2.21 1.04
Vacuole Periphery -1.57 -1.47 -0.12 1.55 1.45 -1.64 1.84 -0.39 1.56 -0.98 -1.99 -0.26 -0.52 1.85 -0.42
Standard name
Human Ortholog
Description Subunit of the Isw1a complex; Isw1a has nucleosome-stimulated ATPase activity and represses transcription initiation by specific positioning of a promoter proximal dinucleosome; promotes nucleosome shifts in the 5 prime direction; IOC3 has a paralog, ESC8, that arose from the whole genome duplication
Localization
Cell Percentages nucleus (99%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Ioc3

Ioc3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ioc3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available