Standard name | |
---|---|
Human Ortholog | |
Description | Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and bud; interacts with Spa2p; YFR016C is not an essential gene |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 14 | 11 | 5 | 15 | 6 | 3 | 7 | 4 | 2 | – | 3 | 8 | 0 | 2 | 3 | 0 | 0 | 0 | 11 | 6 | 8 | 17 | 23 | 25 |
Bud | 4 | 3 | 1 | 7 | 6 | 9 | 10 | 15 | 16 | – | 9 | 13 | 0 | 1 | 9 | 0 | 0 | 0 | 9 | 5 | 4 | 6 | 12 | 17 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 6 | 5 | 5 | 11 | 14 | 9 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 2 | 2 | 2 | 1 | – | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 34 | 15 | 11 | 9 | 15 | 10 | 16 | 12 | 18 | – | 29 | 20 | 8 | 10 | 24 | 9 | 10 | 7 | 1 | 0 | 1 | 1 | 2 | 2 |
Cytoplasm | 275 | 301 | 242 | 156 | 242 | 216 | 263 | 264 | 203 | – | 210 | 201 | 279 | 498 | 518 | 180 | 125 | 148 | 270 | 312 | 235 | 162 | 240 | 251 |
Endoplasmic Reticulum | 16 | 21 | 14 | 14 | 21 | 16 | 11 | 6 | 9 | – | 15 | 32 | 6 | 3 | 4 | 10 | 2 | 0 | 12 | 5 | 12 | 14 | 19 | 30 |
Endosome | 0 | 0 | 0 | 0 | 0 | 2 | 9 | 1 | 2 | – | 0 | 0 | 0 | 1 | 0 | 2 | 0 | 1 | 2 | 6 | 2 | 6 | 6 | 5 |
Golgi | 3 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 3 | 2 | 2 | 3 | 1 | 1 | 0 | 1 | 2 |
Mitochondria | 1 | 3 | 8 | 27 | 28 | 94 | 163 | 201 | 188 | – | 235 | 251 | 2 | 7 | 27 | 4 | 1 | 3 | 1 | 2 | 12 | 9 | 8 | 10 |
Nucleus | 1 | 2 | 2 | 2 | 3 | 4 | 2 | 1 | 4 | – | 0 | 0 | 0 | 1 | 0 | 4 | 1 | 2 | 0 | 0 | 0 | 0 | 1 | 0 |
Nuclear Periphery | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 2 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 |
SpindlePole | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Vac/Vac Membrane | 2 | 1 | 4 | 0 | 10 | 7 | 13 | 19 | 9 | – | 8 | 10 | 1 | 2 | 3 | 18 | 11 | 8 | 3 | 11 | 26 | 12 | 17 | 9 |
Unique Cell Count | 303 | 339 | 271 | 196 | 297 | 303 | 407 | 410 | 348 | 393 | 403 | 285 | 507 | 528 | 200 | 141 | 160 | 325 | 362 | 312 | 246 | 357 | 374 | |
Labelled Cell Count | 351 | 359 | 287 | 230 | 334 | 364 | 497 | 525 | 452 | 511 | 538 | 296 | 526 | 590 | 230 | 152 | 171 | 325 | 362 | 312 | 246 | 357 | 374 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 10.7 | 8.0 | 7.2 | 6.6 | 7.4 | 6.6 | 6.1 | 5.5 | 5.3 | – | 5.2 | 5.0 | 8.1 | 8.6 | 7.9 | 13.4 | 14.5 | 15.2 | 8.2 | 8.0 | 8.3 |
Std Deviation (1e-4) | 1.3 | 1.3 | 1.1 | 1.1 | 1.7 | 1.5 | 1.4 | 1.4 | 1.2 | – | 1.2 | 1.1 | 1.3 | 1.4 | 1.3 | 3.4 | 4.1 | 3.7 | 1.1 | 1.5 | 1.6 |
Intensity Change (Log2) | – | – | – | -0.12 | 0.05 | -0.12 | -0.23 | -0.37 | -0.45 | – | -0.45 | -0.53 | 0.18 | 0.27 | 0.14 | 0.9 | 1.02 | 1.09 | 0.19 | 0.16 | 0.21 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 3.1 | 0.2 | 0 | 0 | 0 | 0 | – | 0 | 0.1 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 2.8 | 3.2 | – | 0 | 2.5 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0.3 | 0.6 | -0.5 | -0.1 | -0.8 | 0.7 | – | 1.8 | 0.5 | -0.8 | -1.7 | 0.3 | 0.2 | 1.3 | 0.2 |
Cytoplasm | -2.9 | -2.6 | -5.4 | -7.2 | -7.3 | -8.5 | – | -9.7 | -10.6 | 4.2 | 5.5 | 5.5 | 0.2 | -0.2 | 1.1 |
Endoplasmic Reticulum | 0.9 | 0.9 | 0.1 | -1.7 | -2.8 | -1.7 | – | -0.8 | 1.4 | -1.9 | -4.2 | -4.0 | -0.1 | -1.9 | -2.9 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 4.4 | 3.2 | 8.8 | 10.9 | 12.8 | 13.6 | – | 14.9 | 15.5 | 0 | -1.5 | 1.4 | -0.6 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 1.5 | 0.7 | 1.4 | 2.2 | 1.0 | – | 0 | 0.9 | 0 | 0 | 0 | 3.8 | 3.3 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 7.2449 | 9.3119 | 8.8945 | 8.5718 | 8.1324 | 8.7074 | 5.4781 | 7.307 | 6.5988 | 6.5443 | 5.3286 | 6.3993 | 4.5759 | 6.3792 | 5.4586 | 5.4895 | 5.2543 | 5.7353 |
Actin | 0.0141 | 0.0084 | 0.0154 | 0.0057 | 0.0028 | 0.003 | 0.0792 | 0.0035 | 0.0345 | 0.0246 | 0.0277 | 0.0043 | 0.0672 | 0.0019 | 0.0641 | 0.0035 | 0.0034 | 0.0197 |
Bud | 0.0148 | 0.006 | 0.0281 | 0.0089 | 0.0019 | 0.0005 | 0.0151 | 0.0145 | 0.0239 | 0.0026 | 0.0016 | 0.0016 | 0.0013 | 0.0007 | 0.0062 | 0.0011 | 0.0016 | 0.0009 |
Bud Neck | 0.0045 | 0.0031 | 0.0113 | 0.0125 | 0.0012 | 0.0723 | 0.0375 | 0.0017 | 0.0012 | 0.0024 | 0.0007 | 0.1013 | 0.0012 | 0.0003 | 0.0021 | 0.0007 | 0.0463 | 0.0826 |
Bud Periphery | 0.0146 | 0.0068 | 0.0218 | 0.0126 | 0.0011 | 0.0006 | 0.0137 | 0.0053 | 0.0131 | 0.0018 | 0.0027 | 0.0007 | 0.0009 | 0.0002 | 0.007 | 0.0006 | 0.0008 | 0.0006 |
Bud Site | 0.06 | 0.1318 | 0.027 | 0.0018 | 0.0009 | 0.0008 | 0.025 | 0.121 | 0.0158 | 0.0079 | 0.0024 | 0.0008 | 0.0046 | 0.0133 | 0.0318 | 0.0025 | 0.018 | 0.0025 |
Cell Periphery | 0.0095 | 0.0054 | 0.0039 | 0.0023 | 0.0004 | 0.001 | 0.0034 | 0.001 | 0.0007 | 0.0007 | 0.0003 | 0.0002 | 0.0011 | 0.0004 | 0.0017 | 0.0003 | 0.0002 | 0.0006 |
Cytoplasm | 0.8091 | 0.7819 | 0.7662 | 0.8644 | 0.8245 | 0.843 | 0.4291 | 0.8213 | 0.7808 | 0.7639 | 0.529 | 0.857 | 0.7171 | 0.911 | 0.7927 | 0.8461 | 0.8542 | 0.83 |
Cytoplasmic Foci | 0.0133 | 0.0092 | 0.0274 | 0.0096 | 0.0311 | 0.0118 | 0.0768 | 0.0082 | 0.0197 | 0.0089 | 0.0405 | 0.0095 | 0.0188 | 0.0332 | 0.0251 | 0.0188 | 0.0322 | 0.0166 |
Eisosomes | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0.0005 | 0 | 0.0003 | 0.0005 | 0.0002 | 0 | 0.0014 | 0 | 0.0003 | 0.0001 | 0 | 0 |
Endoplasmic Reticulum | 0.0269 | 0.0097 | 0.0147 | 0.0128 | 0.02 | 0.023 | 0.0303 | 0.0113 | 0.0054 | 0.0184 | 0.0097 | 0.0132 | 0.0139 | 0.0023 | 0.0042 | 0.0092 | 0.0026 | 0.0034 |
Endosome | 0.0082 | 0.0085 | 0.0226 | 0.0313 | 0.0846 | 0.021 | 0.1118 | 0.0036 | 0.0386 | 0.0243 | 0.0871 | 0.0058 | 0.0556 | 0.018 | 0.0117 | 0.0501 | 0.0249 | 0.0073 |
Golgi | 0.0049 | 0.0041 | 0.0094 | 0.011 | 0.0024 | 0.0059 | 0.0212 | 0.0012 | 0.0091 | 0.0116 | 0.0477 | 0.001 | 0.0281 | 0.0055 | 0.015 | 0.0045 | 0.0047 | 0.004 |
Lipid Particles | 0.0016 | 0.0005 | 0.0043 | 0.0002 | 0.0005 | 0.0064 | 0.0297 | 0.0002 | 0.0037 | 0.0092 | 0.0173 | 0.0001 | 0.0308 | 0.0003 | 0.0041 | 0.0011 | 0.0003 | 0.0001 |
Mitochondria | 0.0009 | 0.007 | 0.01 | 0.0052 | 0.0005 | 0.0035 | 0.009 | 0.0008 | 0.0055 | 0.0412 | 0.1042 | 0.0004 | 0.0182 | 0.0002 | 0.0038 | 0.0018 | 0.0005 | 0.0009 |
None | 0.0061 | 0.0073 | 0.0016 | 0.0018 | 0.0013 | 0.0016 | 0.0206 | 0.0024 | 0.0184 | 0.0038 | 0.0086 | 0.0012 | 0.0212 | 0.004 | 0.002 | 0.0397 | 0.0057 | 0.0287 |
Nuclear Periphery | 0.0017 | 0.0008 | 0.0018 | 0.0013 | 0.001 | 0.0006 | 0.0247 | 0.0005 | 0.0068 | 0.0302 | 0.031 | 0.0006 | 0.0058 | 0.0004 | 0.0005 | 0.0032 | 0.0002 | 0.0002 |
Nucleolus | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0 | 0 | 0.0028 | 0.0001 | 0.0006 | 0.0006 | 0.007 | 0 | 0.0003 | 0 | 0.0001 | 0.0002 | 0.0001 | 0 |
Nucleus | 0.0018 | 0.0018 | 0.0015 | 0.0016 | 0.0009 | 0.001 | 0.0222 | 0.0012 | 0.0084 | 0.0288 | 0.0155 | 0.0008 | 0.0016 | 0.0006 | 0.0006 | 0.0023 | 0.0005 | 0.0003 |
Peroxisomes | 0.0003 | 0.0004 | 0.0156 | 0.0001 | 0.0002 | 0.0004 | 0.0191 | 0.0004 | 0.0012 | 0.0053 | 0.0398 | 0.0002 | 0.0038 | 0.0006 | 0.024 | 0.001 | 0.0012 | 0.0004 |
Punctate Nuclear | 0.0003 | 0.0002 | 0.0014 | 0.0001 | 0.0001 | 0.0001 | 0.0095 | 0.0003 | 0.0016 | 0.0011 | 0.0126 | 0.0002 | 0.0026 | 0.0006 | 0.0006 | 0.0014 | 0.0004 | 0.0001 |
Vacuole | 0.0067 | 0.006 | 0.0132 | 0.0136 | 0.0221 | 0.0029 | 0.015 | 0.0015 | 0.0084 | 0.0085 | 0.0061 | 0.0009 | 0.0029 | 0.0057 | 0.0018 | 0.0095 | 0.0018 | 0.0009 |
Vacuole Periphery | 0.0005 | 0.0008 | 0.0029 | 0.0029 | 0.0027 | 0.0007 | 0.004 | 0.0002 | 0.0022 | 0.0035 | 0.0085 | 0.0002 | 0.0017 | 0.0007 | 0.0004 | 0.0022 | 0.0004 | 0.0002 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 21.311 | 19.7169 | 23.7256 | 20.3912 | 31.4951 | 34.5554 | 22.293 | 25.8195 | 36.399 | 27.4931 |
Translational Efficiency | 3.2186 | 1.9809 | 1.2213 | 2.424 | 1.7647 | 1.3883 | 1.1621 | 1.1187 | 1.0033 | 1.5055 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1927 | 263 | 447 | 955 | 1718 | 1949 | 1564 | 188 | 3645 | 2212 | 2011 | 1143 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 857.99 | 1026.90 | 1422.99 | 962.16 | 829.75 | 970.59 | 1149.37 | 1162.20 | 844.68 | 977.29 | 1210.19 | 995.06 |
Standard Deviation | 109.67 | 134.39 | 187.75 | 119.95 | 118.19 | 132.51 | 137.90 | 153.21 | 114.64 | 133.98 | 188.59 | 146.23 |
Intensity Change Log 2 | — | 0.259263 | 0.729893 | 0.165316 | — | 0.226185 | 0.470095 | 0.486110 | — | 0.243096 | 0.608000 | 0.331936 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000511 | 0.002807 | 0.001277 | 0.000556 | 0.000551 | 0.001353 | 0.001484 | 0.001485 | 0.000530 | 0.001526 | 0.001438 | 0.000709 |
Bud Neck | 0.007114 | 0.011361 | 0.004491 | 0.013923 | 0.010832 | 0.009203 | 0.007463 | 0.006648 | 0.008866 | 0.009460 | 0.006802 | 0.012726 |
Bud Site | 0.061353 | 0.143206* | 0.057931 | 0.130384* | 0.080281 | 0.118618* | 0.120371* | 0.148859* | 0.070274 | 0.121541* | 0.106492* | 0.133423* |
Cell Periphery | 0.000433 | 0.000876 | 0.000300 | 0.000125 | 0.000228 | 0.000360 | 0.000157 | 0.000592 | 0.000336 | 0.000422 | 0.000189 | 0.000202 |
Cytoplasm | 0.665196* | 0.540722* | 0.447251* | 0.373207* | 0.571835* | 0.647836* | 0.700956* | 0.581463* | 0.621192* | 0.635101* | 0.644563* | 0.407461* |
Cytoplasmic Foci | 0.183094* | 0.204764* | 0.098699 | 0.052191 | 0.213402* | 0.156455 | 0.078008 | 0.079688 | 0.197379* | 0.162199 | 0.082607 | 0.056714 |
Eisosomes | 0.000108 | 0.000108 | 0.000022 | 0.000041 | 0.000091 | 0.000101 | 0.000024 | 0.000024 | 0.000100 | 0.000102 | 0.000023 | 0.000039 |
Endoplasmic Reticulum | 0.004422 | 0.001472 | 0.004227 | 0.001280 | 0.002477 | 0.001685 | 0.000431 | 0.000134 | 0.003505 | 0.001660 | 0.001275 | 0.001092 |
Endosome | 0.010445 | 0.015001 | 0.020953 | 0.020317 | 0.022982 | 0.009051 | 0.004509 | 0.005920 | 0.016354 | 0.009759 | 0.008164 | 0.017949 |
Golgi | 0.019907 | 0.013463 | 0.001871 | 0.002689 | 0.018524 | 0.018869 | 0.001326 | 0.012017 | 0.019255 | 0.018226 | 0.001447 | 0.004223 |
Lipid Particles | 0.002298 | 0.000929 | 0.004066 | 0.001947 | 0.002879 | 0.001003 | 0.000865 | 0.000822 | 0.002572 | 0.000994 | 0.001577 | 0.001762 |
Mitochondria | 0.004924 | 0.000807 | 0.000026 | 0.001645 | 0.004514 | 0.003348 | 0.000612 | 0.000020 | 0.004731 | 0.003046 | 0.000482 | 0.001378 |
Mitotic Spindle | 0.000443 | 0.008665 | 0.000806 | 0.014456 | 0.000353 | 0.001835 | 0.001673 | 0.020948 | 0.000400 | 0.002647 | 0.001480 | 0.015524 |
None | 0.001520 | 0.000106 | 0.000164 | 0.000301 | 0.001164 | 0.000366 | 0.000260 | 0.000118 | 0.001352 | 0.000335 | 0.000238 | 0.000271 |
Nuclear Periphery | 0.000079 | 0.000020 | 0.000158 | 0.000261 | 0.000101 | 0.000018 | 0.000030 | 0.000039 | 0.000089 | 0.000018 | 0.000059 | 0.000225 |
Nuclear Periphery Foci | 0.000398 | 0.000397 | 0.003809 | 0.000922 | 0.000309 | 0.000140 | 0.001632 | 0.000695 | 0.000356 | 0.000170 | 0.002116 | 0.000885 |
Nucleolus | 0.000181 | 0.000043 | 0.000115 | 0.000216 | 0.000354 | 0.000092 | 0.000068 | 0.000050 | 0.000263 | 0.000086 | 0.000079 | 0.000189 |
Nucleus | 0.006086 | 0.001846 | 0.007048 | 0.016727 | 0.006791 | 0.002963 | 0.008182 | 0.002466 | 0.006418 | 0.002830 | 0.007930 | 0.014382 |
Peroxisomes | 0.001364 | 0.003993 | 0.001332 | 0.002911 | 0.002243 | 0.002176 | 0.003211 | 0.007083 | 0.001778 | 0.002392 | 0.002794 | 0.003597 |
Vacuole | 0.023056 | 0.046637 | 0.342801* | 0.362386* | 0.050859 | 0.020342 | 0.068321 | 0.130617 | 0.036161 | 0.023469 | 0.129332 | 0.324265* |
Vacuole Periphery | 0.007068 | 0.002774 | 0.002652 | 0.003512 | 0.009230 | 0.004185 | 0.000414 | 0.000314 | 0.008087 | 0.004017 | 0.000912 | 0.002986 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -3.55 | -2.37 | -0.24 | 3.50 | 2.26 | -4.44 | -6.05 | -1.64 | 1.09 | 1.33 | -5.84 | -6.79 | -0.92 | 5.03 | 5.44 |
Bud Neck | -1.72 | 3.02 | -3.00 | -0.24 | -4.83 | 1.39 | 3.82 | 4.20 | 3.43 | 1.49 | -0.67 | 3.71 | -1.41 | -0.95 | -3.54 |
Bud Site | -6.46 | 0.87 | -6.00 | 2.10 | -5.40 | -6.52 | -4.05 | -1.53 | 1.18 | 0.33 | -10.55 | -4.93 | -5.44 | 1.37 | -1.86 |
Cell Periphery | -1.84 | 2.77 | 8.90 | 3.22 | 4.71 | -4.16 | 4.21 | -0.57 | 0.02 | -0.92 | -2.04 | 7.06 | 4.29 | 5.09 | 0.77 |
Cytoplasm | 7.25 | 13.68 | 25.29 | 8.20 | 3.76 | -8.60 | -13.32 | 0.39 | 3.74 | 5.82 | -1.96 | -2.62 | 20.71 | 20.69 | 20.37 |
Cytoplasmic Foci | -1.83 | 13.99 | 30.13 | 13.30 | 8.72 | 9.62 | 25.94 | 15.02 | 8.98 | -0.30 | 7.57 | 31.12 | 37.76 | 24.72 | 8.15 |
Eisosomes | -0.02 | 9.10 | 7.16 | 4.66 | -5.36 | -0.90 | 7.63 | 7.53 | 9.92 | 0.40 | -0.24 | 11.85 | 9.37 | 9.06 | -5.40 |
Endoplasmic Reticulum | 3.68 | 0.68 | 4.46 | 0.60 | 4.01 | 1.65 | 5.29 | 6.05 | 5.50 | 6.59 | 4.02 | 5.79 | 5.08 | 1.73 | 0.68 |
Endosome | -2.57 | -6.92 | -3.65 | -1.02 | 0.93 | 11.13 | 16.03 | 11.35 | 3.12 | -1.00 | 8.26 | 12.05 | 0.48 | -3.50 | -4.85 |
Golgi | 2.65 | 27.45 | 20.17 | 4.54 | -0.77 | -0.34 | 23.36 | 2.33 | 2.39 | -1.03 | 1.12 | 34.80 | 15.00 | 12.13 | -1.61 |
Lipid Particles | 5.49 | -4.36 | 0.93 | -1.15 | 3.32 | 6.57 | 7.30 | 6.06 | 0.11 | -0.37 | 8.03 | 4.96 | 1.82 | -1.04 | 0.10 |
Mitochondria | 4.42 | 5.54 | 3.32 | -0.81 | -2.16 | 1.34 | 5.22 | 6.46 | 6.43 | 1.60 | 2.30 | 6.95 | 4.69 | 2.79 | -1.27 |
Mitotic Spindle | -1.70 | -2.00 | -4.34 | -0.76 | -4.18 | -2.14 | -2.68 | -1.62 | -1.41 | -1.41 | -2.67 | -2.82 | -4.62 | -3.61 | -4.13 |
None | 6.59 | 5.97 | 5.19 | -1.88 | -1.01 | 3.01 | 3.46 | 3.98 | 4.31 | 4.20 | 5.90 | 6.60 | 5.81 | 0.68 | -0.32 |
Nuclear Periphery | 1.33 | -1.98 | -2.26 | -3.83 | -1.22 | 2.45 | 1.78 | 0.85 | -4.74 | -3.07 | 2.50 | 0.68 | -2.25 | -4.04 | -2.98 |
Nuclear Periphery Foci | -0.04 | -9.72 | -2.06 | -2.49 | 8.72 | 2.89 | -10.25 | -4.50 | -5.50 | 3.11 | 1.86 | -12.02 | -3.48 | -5.53 | 7.61 |
Nucleolus | 5.16 | 2.13 | -0.82 | -7.29 | -3.46 | 1.88 | 2.07 | 2.09 | 0.89 | 0.34 | 2.52 | 2.74 | 1.11 | -3.35 | -5.39 |
Nucleus | 8.16 | -2.70 | -9.17 | -13.60 | -6.87 | 5.86 | -4.69 | 3.97 | -1.80 | 9.49 | 7.27 | -6.26 | -8.53 | -12.16 | -5.30 |
Peroxisomes | -5.24 | 0.48 | -3.46 | 2.04 | -3.46 | 0.40 | -2.02 | -1.67 | -1.73 | -1.25 | -4.24 | -3.30 | -3.07 | -1.54 | -1.16 |
Vacuole | -3.95 | -22.31 | -35.64 | -28.42 | -2.00 | 14.05 | -9.48 | -8.82 | -10.53 | -6.81 | 8.28 | -22.34 | -34.75 | -36.17 | -20.43 |
Vacuole Periphery | 7.24 | 8.83 | 4.82 | -0.18 | -0.63 | 7.71 | 15.31 | 15.55 | 12.61 | 1.88 | 9.05 | 19.96 | 7.82 | 2.26 | -2.62 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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