Standard name
Human Ortholog
Description 1-phosphatidylinositol-3-phosphate 5-kinase; vacuolar membrane kinase that generates phosphatidylinositol (3,5)P2, which is involved in vacuolar sorting and homeostasis
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.33 0.56 0.77 0.86 0.81 0.76 0.76 0.72 0.61 – 0.59 0.63 0.74 0.8 0.88 0.52 0.58 0.63 0 0.1 0.16 0.13 0.11 0.14
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0.26 0.1 0 0 0.06 0 0 0 0 – 0 0 0.06 0.05 0 0.17 0.16 0.16 0.3 0.21 0.16 0.14 0.16 0.13
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.05 0.22 0.31 0.07 0.32 0.35 0.35 0.58 – 0.64 0.67 0 0 0 0 0 0 0.08 0.06 0.12 0.05 0.07 0.06
Nucleus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.5 0.39 0.08 0 0.08 0 0.05 0 0 – 0 0 0.22 0.18 0.11 0.42 0.41 0.31 0.5 0.56 0.5 0.61 0.56 0.58
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 2 0 2 1 0 0 0 0 0 – 0 0 3 3 0 5 1 1 1 5 1 3 4 4
Bud 3 5 3 1 4 1 4 4 1 – 5 3 10 7 27 2 0 2 1 6 0 1 7 7
Bud Neck 0 0 0 0 0 0 0 0 1 – 0 0 0 0 0 0 0 1 1 3 0 4 2 3
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 2 3 2 – 2 1 0 0 0 0 0 0 0 0 0 0 0 1
Cytoplasm 94 224 110 107 154 120 136 136 80 – 85 76 426 508 571 321 216 321 15 45 26 73 58 89
Endoplasmic Reticulum 7 7 3 0 4 0 1 3 0 – 3 1 3 10 2 24 14 21 3 4 3 5 15 12
Endosome 73 38 1 0 12 1 0 1 0 – 0 0 35 31 13 106 59 83 106 98 27 74 86 83
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 1 0 0 1 0 2 2 0 1 0 2
Mitochondria 6 20 31 39 14 51 62 66 76 – 91 80 9 8 13 12 9 21 27 27 20 28 38 36
Nucleus 6 1 0 0 0 0 0 1 1 – 0 1 0 2 1 12 1 3 0 1 0 2 1 0
Nuclear Periphery 4 5 0 0 0 0 1 0 1 – 1 0 0 0 0 2 0 0 9 3 2 4 8 7
Nucleolus 3 0 0 0 1 0 0 0 0 – 0 0 0 0 0 1 0 2 0 0 0 1 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 1 0 2 0 0 0 0 0 0 1
SpindlePole 7 0 1 0 0 0 3 0 0 – 0 0 5 5 3 10 3 4 4 2 1 4 2 6
Vac/Vac Membrane 143 154 12 1 15 6 9 5 3 – 3 2 126 112 69 259 153 157 179 264 86 332 299 363
Unique Cell Count 284 397 143 125 189 157 178 188 131 143 120 575 632 649 612 374 512 357 471 172 548 536 631
Labelled Cell Count 348 454 163 149 204 179 218 219 165 190 164 617 687 700 754 459 616 357 471 172 548 536 631


Vacuole Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.9 4.6 3.8 3.7 4.1 3.7 3.6 3.4 3.1 – 3.2 2.9 4.6 4.7 4.4 6.8 7.2 7.0 4.8 4.9 5.0
Std Deviation (1e-4) 0.7 0.8 0.6 0.7 0.6 0.8 1.0 0.4 0.4 – 0.7 0.4 0.9 1.0 1.0 1.4 1.4 1.5 1.0 1.2 1.0
Intensity Change (Log2) – – – -0.03 0.13 -0.03 -0.07 -0.14 -0.27 – -0.26 -0.37 0.3 0.31 0.23 0.86 0.94 0.89 0.35 0.38 0.4


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 7.6 7.1 5.4

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.5257 4.258 4.0509 4.3495 3.7412 4.3092 -1.2663 -1.0063 -1.014 -1.179 -1.002 -0.9847 3.455 3.9638 3.8772 4.1009 4.0674 4.3114
Actin 0.0214 0.0006 0.0057 0.0011 0.0128 0.007 0.003 0.001 0.0089 0.0004 0.0036 0.004 0.0066 0.0013 0.0002 0.0007 0.0001 0.0021
Bud 0.0011 0.0058 0.0053 0.0005 0.001 0.0011 0.0079 0.0054 0.0104 0.0201 0.0228 0.0081 0.0016 0.0009 0.0031 0.0026 0.001 0.0017
Bud Neck 0.0044 0.0005 0.0021 0.0003 0.0014 0.0099 0.003 0.002 0.0011 0.0028 0.0196 0.0033 0.0012 0.0032 0.0006 0.0008 0.0004 0.0058
Bud Periphery 0.0034 0.0096 0.0043 0.0013 0.0018 0.0013 0.005 0.0031 0.005 0.0072 0.0302 0.0116 0.0022 0.0013 0.0024 0.0032 0.0024 0.0036
Bud Site 0.0069 0.0048 0.004 0.0011 0.0015 0.0013 0.0035 0.0182 0.0181 0.0043 0.075 0.0004 0.0049 0.0032 0.0027 0.0015 0.0004 0.0007
Cell Periphery 0.0014 0.001 0.0012 0.0004 0.0005 0.0004 0.0008 0.0006 0.0004 0.0005 0.0014 0.0002 0.0004 0.0003 0.0004 0.0003 0.0001 0.0003
Cytoplasm 0.2168 0.3702 0.3371 0.2027 0.2945 0.3924 0.2181 0.3581 0.2283 0.228 0.1982 0.3919 0.1716 0.3188 0.1872 0.1897 0.2206 0.2909
Cytoplasmic Foci 0.0751 0.0531 0.0832 0.0604 0.1034 0.066 0.0711 0.0731 0.0664 0.0779 0.0588 0.0778 0.0928 0.0627 0.0951 0.0848 0.0633 0.0893
Eisosomes 0.001 0.0001 0.0002 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0 0 0 0.0001 0.0001 0.0001 0 0 0.0001
Endoplasmic Reticulum 0.0175 0.0075 0.0142 0.0022 0.0061 0.0077 0.0104 0.0061 0.0065 0.0035 0.0056 0.0088 0.0045 0.0084 0.0101 0.0048 0.0058 0.0043
Endosome 0.2115 0.1783 0.2097 0.2843 0.2279 0.218 0.2459 0.1922 0.3322 0.3488 0.3259 0.2107 0.3267 0.2323 0.3549 0.3674 0.3515 0.2606
Golgi 0.013 0.0032 0.0068 0.0069 0.0076 0.0039 0.0046 0.0027 0.0052 0.0065 0.0051 0.0027 0.0068 0.0056 0.0075 0.0051 0.0043 0.0043
Lipid Particles 0.0185 0.0028 0.0091 0.002 0.0238 0.003 0.017 0.003 0.002 0.0029 0.0184 0.0024 0.0091 0.0054 0.0038 0.0041 0.0029 0.0045
Mitochondria 0.0127 0.0052 0.0149 0.0351 0.0098 0.0054 0.0146 0.0028 0.0128 0.0069 0.0022 0.0036 0.0047 0.0028 0.0091 0.0026 0.0066 0.0062
None 0.206 0.1897 0.1343 0.2224 0.1384 0.1436 0.2306 0.2175 0.1449 0.1379 0.0333 0.1551 0.1951 0.2005 0.1714 0.1374 0.1802 0.207
Nuclear Periphery 0.0152 0.0047 0.0094 0.0021 0.0093 0.003 0.0186 0.0064 0.0142 0.003 0.0086 0.0143 0.003 0.0101 0.0054 0.0032 0.0109 0.0057
Nucleolus 0.0022 0.0011 0.0023 0.0004 0.0015 0.0006 0.0026 0.0021 0.001 0.0005 0.0009 0.0006 0.0017 0.0005 0.0023 0.0011 0.0006 0.0008
Nucleus 0.0341 0.005 0.0068 0.0019 0.0066 0.0044 0.0277 0.0086 0.0072 0.0039 0.0177 0.0081 0.0038 0.0044 0.0059 0.0028 0.007 0.0048
Peroxisomes 0.0062 0.0006 0.0029 0.0048 0.0117 0.0012 0.0075 0.0011 0.0007 0.0008 0.0012 0.0004 0.0046 0.0008 0.0026 0.0013 0.0004 0.0031
Punctate Nuclear 0.0099 0.001 0.005 0.0005 0.0072 0.0042 0.0065 0.0052 0.0023 0.0017 0.0075 0.0078 0.0019 0.0007 0.0024 0.0018 0.0009 0.0027
Vacuole 0.1098 0.1404 0.1222 0.1477 0.1098 0.1023 0.09 0.0777 0.1011 0.1159 0.1187 0.0698 0.1414 0.1145 0.1141 0.1461 0.1217 0.0848
Vacuole Periphery 0.0122 0.0149 0.0195 0.0215 0.0236 0.0233 0.0113 0.013 0.0311 0.0267 0.0455 0.0182 0.0153 0.022 0.0186 0.0387 0.0187 0.0167

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 12.911 16.014 8.0291 15.8549 13.7056 13.0651 21.1878 17.1309 15.4433 20.2835
Translational Efficiency 0.3809 0.3935 0.4573 0.2327 0.2901 0.4336 0.3303 0.2975 0.3423 0.265

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1916 1219 735 1111 911 1246 879 336 2827 2465 1614 1447

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 609.39 698.58 921.21 836.22 687.03 692.16 842.83 866.51 634.41 695.33 878.52 843.25
Standard Deviation 69.41 90.33 116.56 117.98 73.61 91.43 99.03 117.47 79.55 90.94 114.24 118.56
Intensity Change Log 2 — 0.197060 0.596164 0.456517 — 0.010732 0.294869 0.334843 — 0.101320 0.444350 0.393317

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000188 0.000217 0.000164 0.000707 0.000144 0.000131 0.000161 0.000169 0.000174 0.000173 0.000162 0.000582
Bud Neck 0.033761 0.019417 0.022944 0.019352 0.019266 0.022698 0.021223 0.028690 0.029090 0.021076 0.022006 0.021520
Bud Site 0.008159 0.018634 0.011072 0.036285 0.007969 0.015320 0.009539 0.025211 0.008097 0.016959 0.010237 0.033713
Cell Periphery 0.000130 0.000069 0.000099 0.000070 0.000103 0.000072 0.000071 0.000049 0.000121 0.000070 0.000083 0.000065
Cytoplasm 0.262623* 0.251251* 0.111883* 0.284228* 0.290012* 0.153537* 0.258131* 0.205712* 0.271449* 0.201859* 0.191531* 0.265996*
Cytoplasmic Foci 0.322505* 0.235522* 0.041243 0.066132 0.265435* 0.226409* 0.041475 0.064323 0.304114* 0.230916* 0.041370 0.065712
Eisosomes 0.000206 0.000058 0.000059 0.000027 0.000116 0.000062 0.000039 0.000031 0.000177 0.000060 0.000048 0.000028
Endoplasmic Reticulum 0.003658 0.002200 0.007713 0.001862 0.004294 0.002073 0.004805 0.001878 0.003863 0.002136 0.006129 0.001866
Endosome 0.095428 0.136687 0.077402 0.068400 0.095902 0.198565 0.059492 0.098637 0.095581 0.167965 0.067648 0.075421
Golgi 0.010186 0.006977 0.000878 0.003667 0.005237 0.012101 0.000578 0.001156 0.008591 0.009567 0.000715 0.003084
Lipid Particles 0.029339 0.010188 0.007434 0.002520 0.015815 0.015588 0.004234 0.002287 0.024981 0.012917 0.005691 0.002465
Mitochondria 0.019562 0.010291 0.001647 0.006417 0.006980 0.020282 0.002189 0.004499 0.015507 0.015341 0.001942 0.005972
Mitotic Spindle 0.002294 0.009629 0.009497 0.034100 0.001555 0.007785 0.010823 0.021134 0.002056 0.008697 0.010219 0.031089
None 0.002756 0.001395 0.001325 0.003812 0.000624 0.000841 0.002225 0.001000 0.002069 0.001115 0.001815 0.003159
Nuclear Periphery 0.000714 0.000827 0.008468 0.002171 0.000744 0.001255 0.005796 0.002420 0.000723 0.001043 0.007013 0.002229
Nuclear Periphery Foci 0.000541 0.000926 0.006763 0.001716 0.000502 0.001044 0.003882 0.002158 0.000528 0.000986 0.005194 0.001818
Nucleolus 0.002264 0.002671 0.015139 0.003779 0.000984 0.002450 0.009167 0.004769 0.001852 0.002559 0.011887 0.004009
Nucleus 0.054274 0.028475 0.195063* 0.051975 0.048510 0.022582 0.189221* 0.046178 0.052416 0.025496 0.191882* 0.050629
Peroxisomes 0.013448 0.009301 0.003452 0.005660 0.006283 0.007782 0.002494 0.004839 0.011139 0.008533 0.002930 0.005469
Vacuole 0.129485 0.236799* 0.456374* 0.397941* 0.221043* 0.264073* 0.370119* 0.475492* 0.158990 0.250586* 0.409399* 0.415949*
Vacuole Periphery 0.008481 0.018467 0.021383 0.009180 0.008482 0.025349 0.004336 0.009370 0.008481 0.021946 0.012099 0.009224

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -0.75 0.26 -1.96 -1.75 -2.01 0.50 -0.98 -1.73 -2.40 -1.15 -0.03 -0.19 -2.11 -2.10 -2.09
Bud Neck 6.38 3.63 6.85 0.69 2.43 -1.49 -1.15 -2.08 -1.25 -1.44 4.59 3.31 4.45 0.36 1.25
Bud Site -5.57 -1.92 -6.94 -2.99 -5.44 -3.58 -0.60 -4.03 -2.01 -3.69 -6.55 -1.69 -7.93 -3.94 -6.78
Cell Periphery 7.63 3.24 8.46 1.77 4.06 3.36 4.34 6.77 2.87 3.02 7.87 5.93 10.11 2.67 4.27
Cytoplasm 1.02 16.42 -2.80 -3.36 -16.19 13.37 3.07 4.37 -4.56 2.05 10.21 10.60 -0.30 -8.33 -8.93
Cytoplasmic Foci 11.76 51.02 48.59 30.23 -3.49 4.55 32.78 26.35 25.11 -3.98 13.24 61.14 55.01 40.91 -5.26
Eisosomes 14.75 14.59 18.66 11.43 9.25 6.71 9.76 10.82 8.69 2.98 16.04 17.78 21.26 15.35 10.20
Endoplasmic Reticulum 3.19 -6.83 4.72 1.47 13.14 4.15 -0.94 4.44 0.60 8.21 5.02 -5.58 6.42 1.78 15.66
Endosome -6.75 4.31 10.82 15.25 4.65 -14.18 7.66 2.81 14.31 -2.77 -15.50 9.08 10.90 24.19 1.90
Golgi 3.83 15.39 8.74 4.83 -2.03 -5.83 8.47 7.32 10.49 -1.20 -1.12 17.66 9.65 9.23 -2.35
Lipid Particles 11.99 14.51 19.37 9.73 7.12 0.11 8.59 10.08 11.23 3.99 9.55 17.80 21.65 14.66 7.98
Mitochondria 5.41 13.45 8.34 3.50 -1.93 -6.21 3.83 1.54 6.06 -0.55 0.20 13.21 7.82 7.36 -1.82
Mitotic Spindle -3.69 -4.30 -9.18 -5.90 -5.05 -4.32 -4.36 -3.96 -2.73 -1.36 -5.46 -5.91 -10.01 -7.01 -4.82
None 2.20 2.19 -0.04 -1.43 -1.48 -1.56 -4.04 -2.09 -0.97 2.48 2.26 0.69 -0.40 -1.70 -0.85
Nuclear Periphery -1.06 -8.99 -10.46 -9.52 6.56 -2.08 -7.01 -4.54 -3.09 3.31 -2.38 -11.33 -11.10 -7.82 7.32
Nuclear Periphery Foci -2.23 -5.66 -6.54 -3.67 4.56 -2.60 -6.75 -5.07 -3.78 1.65 -3.18 -8.12 -8.44 -5.27 5.44
Nucleolus -1.44 -8.87 -1.98 -0.69 7.90 -4.69 -6.82 -4.33 -2.86 2.58 -2.71 -10.45 -4.31 -2.45 7.85
Nucleus 8.85 -15.08 -3.13 -9.78 13.24 7.94 -17.97 -2.07 -6.17 13.45 12.43 -23.32 -3.92 -12.36 19.70
Peroxisomes 3.22 10.18 8.55 5.78 -1.07 -1.50 4.69 2.09 3.87 -2.32 2.87 11.60 8.51 6.68 -2.32
Vacuole -14.76 -29.50 -31.13 -17.84 1.36 -4.31 -12.82 -14.85 -12.51 -5.69 -15.83 -31.72 -33.16 -21.27 -3.32
Vacuole Periphery -6.67 -6.51 3.18 8.14 7.84 -10.08 5.41 1.54 10.79 -2.73 -12.28 -2.60 3.51 13.95 5.05
Standard name
Human Ortholog
Description 1-phosphatidylinositol-3-phosphate 5-kinase; vacuolar membrane kinase that generates phosphatidylinositol (3,5)P2, which is involved in vacuolar sorting and homeostasis
Localization
Cell Percentages —
Cell Cycle Regulation No
Subcompartmental Group N/A

Fab1

Fab1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Fab1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available