Standard name
Human Ortholog
Description Histidinolphosphatase; catalyzes the eighth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0
Cytoplasm 0.98 0.97 0.97 0.88 0.89 0.82 0.72 0.73 0.69 0.7 0.68 0.71 0.96 0.99 0.98 0.95 0.96 0.93 0.97 0.89 0.9 0.95 0.96 0.95
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.08 0.12 0.07 0.15 0.13 0.24 0.18 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0.06 0 0.05 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0.13 0.11 0.09 0.19 0.16 0.18 0.15 0.1 0.16 0 0 0 0.05 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0
Bud 0 0 0 0 0 0 1 1 1 3 4 2 0 0 0 0 0 1 0 0 0 0 0 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 2 1 2 7 3 3 4 4 3 1 0 0 3 10 10 6 0 0 0 0 0 0
Cytoplasm 168 86 139 109 200 212 193 146 125 164 99 120 265 471 414 258 151 137 163 86 142 228 391 472
Endoplasmic Reticulum 0 2 0 1 2 5 3 2 1 4 3 1 3 0 2 7 2 6 0 1 3 0 2 6
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 2 0 1 1 1
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0 0 1 0
Mitochondria 1 0 0 5 2 20 32 14 27 31 35 31 0 0 0 1 2 1 0 1 1 0 1 3
Nucleus 1 0 4 2 5 6 8 13 3 11 7 6 2 2 0 3 1 3 0 2 2 4 2 0
Nuclear Periphery 1 0 0 0 0 0 0 0 0 1 0 0 1 0 0 0 0 0 0 0 0 0 1 0
Nucleolus 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 1 1 2 16 24 24 51 32 32 35 14 27 9 13 10 14 5 5 0 1 1 2 2 1
Unique Cell Count 171 89 144 124 224 258 267 201 180 234 146 170 275 476 424 271 157 147 169 97 159 241 408 496
Labelled Cell Count 172 89 147 135 235 274 291 213 194 253 165 188 282 486 430 293 171 159 169 97 159 241 408 496


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.9 11.7 9.1 9.0 10.0 8.8 7.9 8.5 7.8 7.4 7.1 7.6 10.2 11.3 11.2 14.6 14.8 14.8 10.4 9.9 10.7
Std Deviation (1e-4) 1.2 1.9 1.8 1.7 1.7 1.5 1.6 1.5 1.7 1.3 1.3 1.4 1.6 1.5 1.6 2.6 2.8 2.7 1.6 2.0 1.8
Intensity Change (Log2) – – – -0.02 0.14 -0.05 -0.21 -0.11 -0.23 -0.3 -0.37 -0.26 0.16 0.31 0.29 0.68 0.7 0.7 0.19 0.12 0.24


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 2.2 0
Cytoplasm -2.7 -2.5 -4.1 -6.0 -5.8 -6.2 -6.3 -6.4 -6.0 -0.1 2.0 0.7 -0.6 -0.2 -1.3
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 3.4 4.3 3.2 4.9 4.6 6.3 5.4 0 0 0 0 0 0
Nucleus 0 0 0 0 1.6 0 0.9 0.9 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 3.8 3.4 3.1 5.1 4.5 4.8 4.3 3.1 4.4 0 0 0 1.9 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.5071 6.2389 5.2822 4.4352 4.7708 5.4228 5.743 6.9732 6.3329 6.0697 5.7022 6.4657 5.2838 6.7804 6.1338 4.7673 5.1934 6.111
Actin 0.0083 0.0003 0.0044 0.0365 0.0029 0.0002 0.004 0.0003 0.0008 0.0002 0.0059 0.0014 0.0191 0.0003 0.0027 0.0026 0.0129 0.0013
Bud 0.003 0.0002 0.0002 0.0013 0.0001 0.0012 0.0005 0.0004 0.0001 0.0001 0.0005 0.0002 0.0004 0.0001 0.001 0.0004 0.0005 0.0002
Bud Neck 0.0019 0.0002 0.0004 0.0007 0.0005 0.0008 0.0008 0.0005 0.0005 0.0007 0.0027 0.001 0.0017 0.0002 0.0008 0.0006 0.0011 0.0009
Bud Periphery 0.0011 0 0.0001 0.0006 0 0.0003 0.0002 0.0001 0 0.0001 0.0005 0.0001 0.0003 0 0.001 0.0003 0.0006 0.0001
Bud Site 0.0009 0.0005 0.0004 0.0014 0 0.0001 0.0007 0.004 0.0002 0.0002 0.0019 0.0001 0.0007 0.0002 0.0032 0.0019 0.0019 0.0001
Cell Periphery 0.0001 0 0 0.0001 0 0 0 0 0 0 0 0 0.0001 0 0.0001 0.0001 0.0002 0
Cytoplasm 0.8521 0.9526 0.927 0.879 0.9034 0.9282 0.907 0.9546 0.9252 0.9133 0.8707 0.9185 0.8596 0.9794 0.9384 0.768 0.7253 0.9317
Cytoplasmic Foci 0.0127 0.0056 0.011 0.0107 0.0055 0.0051 0.0108 0.0048 0.0076 0.0062 0.022 0.0057 0.0203 0.0027 0.009 0.0528 0.0236 0.0081
Eisosomes 0.0001 0 0 0.0001 0 0 0 0 0 0 0 0 0.0001 0 0 0 0.0002 0
Endoplasmic Reticulum 0.0046 0.0013 0.0021 0.0027 0.0026 0.0023 0.003 0.0011 0.0013 0.0021 0.002 0.0018 0.0038 0.0013 0.0013 0.0024 0.0135 0.0019
Endosome 0.0144 0.0031 0.0046 0.0066 0.0042 0.0036 0.0098 0.0015 0.0022 0.0262 0.0098 0.0049 0.0197 0.0025 0.0025 0.0998 0.0783 0.0052
Golgi 0.0017 0.0001 0.0019 0.0042 0.0014 0.0001 0.0017 0.0001 0.0006 0.0007 0.005 0.0017 0.0069 0.0001 0.0008 0.0163 0.0113 0.0013
Lipid Particles 0.0035 0 0.0012 0.0007 0.0091 0 0.0019 0 0.001 0.0004 0.0107 0.002 0.0048 0 0.0019 0.0098 0.0275 0.0005
Mitochondria 0.0047 0.0002 0.0007 0.0034 0.0006 0.0002 0.001 0.0002 0.0003 0.0004 0.0023 0.0006 0.0168 0.0001 0.0003 0.0027 0.0033 0.0027
None 0.0237 0.0058 0.0069 0.0075 0.0032 0.0047 0.0204 0.0098 0.0128 0.0101 0.0144 0.008 0.0056 0.0029 0.0065 0.0035 0.012 0.0024
Nuclear Periphery 0.0063 0.0027 0.0025 0.0051 0.0048 0.0035 0.0034 0.0014 0.0022 0.0017 0.0026 0.0045 0.0055 0.0013 0.002 0.0063 0.0228 0.003
Nucleolus 0.0006 0.0001 0 0.0001 0 0 0.0001 0 0.0001 0.0001 0.0004 0.0001 0.0001 0 0.0001 0.0007 0.0008 0
Nucleus 0.0466 0.024 0.0308 0.0347 0.0393 0.0467 0.0238 0.0186 0.0396 0.0313 0.0269 0.0454 0.0267 0.008 0.0237 0.0111 0.043 0.0341
Peroxisomes 0.0064 0.0001 0.0032 0.0012 0.0186 0.0001 0.0077 0.0002 0.0025 0.0002 0.0152 0.0003 0.0038 0 0.0022 0.0107 0.0058 0.001
Punctate Nuclear 0.0039 0.0017 0.0013 0.0025 0.0027 0.002 0.0022 0.0016 0.0024 0.0015 0.0052 0.0027 0.0021 0.0003 0.0018 0.0057 0.0106 0.0044
Vacuole 0.0026 0.001 0.0009 0.0008 0.0007 0.0009 0.0008 0.0007 0.0005 0.004 0.0011 0.0008 0.0015 0.0004 0.0006 0.0029 0.0033 0.0007
Vacuole Periphery 0.0008 0.0002 0.0002 0.0002 0.0003 0.0002 0.0002 0.0001 0.0001 0.0006 0.0003 0.0003 0.0006 0.0001 0.0001 0.0014 0.0015 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 20.7983 36.2063 19.6054 25.6679 12.7733 21.8808 26.2193 30.3842 26.1433 28.0509
Translational Efficiency 1.0052 0.5717 0.987 0.8261 1.5391 0.9713 1.0901 0.8141 0.9743 0.7339

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Histidinolphosphatase; catalyzes the eighth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

His2

His2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (His2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available