Standard name | |
---|---|
Human Ortholog | |
Description | Type I membrane protein with a J domain; required to preserve the folding capacity of the endoplasmic reticulum; loss of the non-essential ERJ5 gene leads to a constitutively induced unfolded protein response |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | – | 6 | 12 | 22 | 12 | 11 | 15 | – | 10 | 15 | 17 | 16 | 10 | 15 | 6 | 9 | 2 | 8 | 0 | 4 | 3 | 3 | 4 | 1 |
Bud | – | 6 | 0 | 3 | 1 | 2 | 3 | – | 7 | 13 | 9 | 14 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 2 | 0 | 1 | 1 | 0 |
Bud Neck | – | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 1 | 1 | 1 |
Bud Site | – | 0 | 0 | 0 | 0 | 0 | 1 | – | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | – | 8 | 6 | 18 | 17 | 8 | 5 | – | 10 | 11 | 20 | 15 | 20 | 38 | 52 | 11 | 12 | 8 | 0 | 1 | 1 | 2 | 2 | 1 |
Cytoplasm | – | 84 | 35 | 55 | 65 | 81 | 99 | – | 75 | 111 | 140 | 145 | 68 | 137 | 185 | 114 | 55 | 109 | 0 | 30 | 5 | 28 | 48 | 22 |
Endoplasmic Reticulum | – | 107 | 141 | 100 | 113 | 72 | 111 | – | 101 | 118 | 59 | 91 | 176 | 248 | 355 | 5 | 2 | 6 | 14 | 191 | 192 | 246 | 246 | 242 |
Endosome | – | 0 | 4 | 3 | 1 | 5 | 7 | – | 1 | 1 | 6 | 3 | 0 | 1 | 0 | 2 | 6 | 8 | 0 | 3 | 1 | 3 | 1 | 1 |
Golgi | – | 0 | 0 | 1 | 1 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 2 | 0 | 1 | 0 | 1 | 1 | 3 |
Mitochondria | – | 7 | 0 | 3 | 1 | 6 | 8 | – | 25 | 36 | 59 | 65 | 0 | 0 | 0 | 2 | 3 | 6 | 0 | 16 | 6 | 7 | 8 | 9 |
Nucleus | – | 0 | 1 | 0 | 2 | 0 | 2 | – | 0 | 1 | 2 | 5 | 0 | 1 | 3 | 0 | 0 | 2 | 0 | 0 | 1 | 0 | 1 | 0 |
Nuclear Periphery | – | 0 | 2 | 1 | 0 | 0 | 2 | – | 10 | 6 | 2 | 5 | 0 | 1 | 1 | 0 | 2 | 1 | 0 | 0 | 2 | 0 | 0 | 0 |
Nucleolus | – | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | – | 0 | 0 | 0 | 1 | 1 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | – | 1 | 1 | 0 | 1 | 0 | 0 | – | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | – | 15 | 18 | 17 | 42 | 41 | 49 | – | 17 | 27 | 38 | 57 | 1 | 3 | 32 | 13 | 3 | 31 | 0 | 17 | 3 | 12 | 15 | 10 |
Unique Cell Count | 212 | 187 | 187 | 220 | 201 | 268 | 214 | 291 | 300 | 352 | 243 | 381 | 550 | 145 | 81 | 162 | 16 | 274 | 223 | 313 | 336 | 295 | ||
Labelled Cell Count | 234 | 220 | 223 | 257 | 227 | 302 | 257 | 339 | 354 | 417 | 275 | 445 | 634 | 158 | 86 | 183 | 16 | 274 | 223 | 313 | 336 | 295 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | – | 6.6 | 9.3 | 9.1 | 10.6 | 9.3 | 7.7 | – | 7.1 | 6.8 | 6.7 | 7.1 | 8.8 | 8.9 | 8.4 | 8.2 | 10.5 | 10.4 | 9.3 | 9.4 | 9.5 |
Std Deviation (1e-4) | – | 1.2 | 1.8 | 1.5 | 9.0 | 10.4 | 1.4 | – | 1.3 | 1.1 | 1.3 | 1.4 | 1.4 | 1.5 | 1.4 | 1.5 | 1.7 | 2.3 | 1.3 | 1.5 | 1.5 |
Intensity Change (Log2) | – | – | – | -0.04 | 0.19 | 0.0 | -0.28 | – | -0.39 | -0.46 | -0.46 | -0.4 | -0.07 | -0.06 | -0.15 | -0.18 | 0.17 | 0.16 | -0.01 | 0.01 | 0.02 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 1.8 | -0.4 | -0.4 | -0.4 | – | -0.8 | -0.6 | -0.3 | -0.9 | -1.1 | -1.3 | 0 | -0.1 | 0 | -0.6 |
Bud | 0 | 0 | 0 | 0 | – | 0 | 2.9 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 2.5 | 2.0 | 0.4 | 0 | – | 0.7 | 0.3 | 1.7 | 0.6 | 2.2 | 2.8 | 2.7 | 1.8 | 3.5 | 0.8 |
Cytoplasm | 2.4 | 2.5 | 4.6 | 4.2 | – | 3.7 | 4.5 | 6.3 | 5.3 | 2.2 | 4.2 | 3.9 | 10.9 | 7.8 | 9.2 |
Endoplasmic Reticulum | -4.4 | -5.0 | -7.8 | -7.2 | – | -5.8 | -7.5 | -12.2 | -11.1 | -0.7 | -2.5 | -2.7 | -13.1 | -11.0 | -13.5 |
Endosome | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.4 |
Golgi | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | – | 4.8 | 5.0 | 6.5 | 6.3 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | – | 2.1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -0.2 | 2.7 | 3.0 | 2.6 | – | -0.6 | -0.1 | 1.0 | 2.1 | -4.6 | -5.2 | -1.8 | -0.2 | -1.7 | 2.6 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 7.9762 | 9.4226 | 9.1536 | 8.5242 | 8.3338 | 8.7604 | 6.344 | 7.6014 | 7.6674 | 6.7421 | 7.7906 | 7.2511 | 3.4756 | 4.9033 | 4.7469 | 3.9385 | 4.4906 | 4.4988 |
Actin | 0.0223 | 0.0009 | 0.0093 | 0.0008 | 0.0334 | 0.0041 | 0.047 | 0.0004 | 0.0132 | 0.0175 | 0.0124 | 0.0062 | 0.032 | 0.0013 | 0.0125 | 0.0184 | 0 | 0.0074 |
Bud | 0.0002 | 0.0001 | 0.0002 | 0 | 0.001 | 0.0001 | 0.0009 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0 | 0.0003 | 0.0001 | 0.0001 | 0.0003 | 0 | 0 |
Bud Neck | 0.0049 | 0.0002 | 0.0041 | 0.0003 | 0.0008 | 0.0011 | 0.0029 | 0.0001 | 0.0003 | 0.0011 | 0.0004 | 0.0008 | 0.0007 | 0.0003 | 0.0004 | 0.0029 | 0.0002 | 0.0078 |
Bud Periphery | 0.0004 | 0.0002 | 0.0003 | 0.0002 | 0.0032 | 0.0002 | 0.0013 | 0.0002 | 0.0001 | 0.0008 | 0.0003 | 0 | 0.0005 | 0.0002 | 0.0002 | 0.0005 | 0 | 0.0001 |
Bud Site | 0.0026 | 0.0014 | 0.0042 | 0 | 0.0019 | 0.0001 | 0.0061 | 0.0003 | 0.0007 | 0.0006 | 0.0003 | 0.0001 | 0.0007 | 0.0019 | 0.001 | 0.0009 | 0 | 0.0001 |
Cell Periphery | 0.002 | 0.0042 | 0.0037 | 0.0011 | 0.0009 | 0.0008 | 0.0017 | 0.002 | 0.0017 | 0.006 | 0.0012 | 0.0007 | 0.0007 | 0.0041 | 0.0011 | 0.0019 | 0.0001 | 0.0004 |
Cytoplasm | 0.057 | 0.1664 | 0.1038 | 0.0787 | 0.0673 | 0.0828 | 0.059 | 0.0951 | 0.1135 | 0.0363 | 0.0754 | 0.0876 | 0.0578 | 0.158 | 0.1188 | 0.047 | 0.0692 | 0.0906 |
Cytoplasmic Foci | 0.0107 | 0.0029 | 0.0071 | 0.0023 | 0.007 | 0.0038 | 0.022 | 0.0021 | 0.0096 | 0.0153 | 0.0089 | 0.0025 | 0.0126 | 0.0062 | 0.0047 | 0.01 | 0.0003 | 0.002 |
Eisosomes | 0.0004 | 0 | 0.0002 | 0.0001 | 0.0005 | 0.0001 | 0.0007 | 0 | 0.0001 | 0.0005 | 0.0004 | 0 | 0.0004 | 0 | 0.0001 | 0.0001 | 0 | 0.0002 |
Endoplasmic Reticulum | 0.7625 | 0.7825 | 0.788 | 0.8435 | 0.7185 | 0.8493 | 0.6908 | 0.8476 | 0.8064 | 0.8006 | 0.7794 | 0.8664 | 0.7477 | 0.7658 | 0.8168 | 0.8502 | 0.9209 | 0.863 |
Endosome | 0.0297 | 0.01 | 0.0142 | 0.0119 | 0.0406 | 0.0189 | 0.0352 | 0.0106 | 0.0153 | 0.0311 | 0.0167 | 0.005 | 0.0413 | 0.0091 | 0.0112 | 0.0158 | 0.0019 | 0.0086 |
Golgi | 0.0103 | 0.0008 | 0.0063 | 0.0023 | 0.0279 | 0.003 | 0.0164 | 0.0049 | 0.0075 | 0.0129 | 0.007 | 0.0017 | 0.0103 | 0.0015 | 0.0033 | 0.0153 | 0.0001 | 0.001 |
Lipid Particles | 0.029 | 0.0048 | 0.0098 | 0.0042 | 0.027 | 0.0057 | 0.0235 | 0.0076 | 0.0101 | 0.0134 | 0.016 | 0.0037 | 0.0137 | 0.0089 | 0.0058 | 0.0153 | 0.0007 | 0.0018 |
Mitochondria | 0.0126 | 0.0002 | 0.015 | 0.0301 | 0.0124 | 0.0047 | 0.0072 | 0.0066 | 0.004 | 0.0119 | 0.0436 | 0.0004 | 0.0083 | 0.0031 | 0.0018 | 0.0025 | 0 | 0.0005 |
None | 0.0202 | 0.0025 | 0.006 | 0.0017 | 0.0026 | 0.0117 | 0.0208 | 0.0061 | 0.0105 | 0.0336 | 0.0167 | 0.0093 | 0.026 | 0.019 | 0.0034 | 0.0007 | 0.0001 | 0.0038 |
Nuclear Periphery | 0.0172 | 0.0137 | 0.0095 | 0.0132 | 0.0339 | 0.0068 | 0.0294 | 0.006 | 0.0036 | 0.0055 | 0.0053 | 0.0056 | 0.0283 | 0.0111 | 0.0092 | 0.0061 | 0.0029 | 0.0079 |
Nucleolus | 0.0005 | 0 | 0.0024 | 0 | 0.0002 | 0.0001 | 0.0005 | 0 | 0 | 0.0001 | 0.0001 | 0 | 0.0004 | 0.0001 | 0.0002 | 0.0001 | 0 | 0 |
Nucleus | 0.0019 | 0.0019 | 0.0014 | 0.0015 | 0.005 | 0.0015 | 0.007 | 0.0008 | 0.0003 | 0.0006 | 0.0007 | 0.005 | 0.0027 | 0.0021 | 0.0013 | 0.0007 | 0.0013 | 0.0012 |
Peroxisomes | 0.0077 | 0.0001 | 0.0066 | 0 | 0.0049 | 0.0005 | 0.012 | 0 | 0.0004 | 0.0045 | 0.0078 | 0.0012 | 0.0023 | 0.0001 | 0.001 | 0.0072 | 0 | 0.0004 |
Punctate Nuclear | 0.0019 | 0.0004 | 0.0033 | 0.0001 | 0.0014 | 0.0003 | 0.0083 | 0.0002 | 0.0002 | 0.0029 | 0.0014 | 0.0004 | 0.0069 | 0.0007 | 0.0042 | 0.0008 | 0 | 0.0003 |
Vacuole | 0.0049 | 0.0061 | 0.004 | 0.0071 | 0.0064 | 0.0038 | 0.0051 | 0.0083 | 0.0023 | 0.0038 | 0.0045 | 0.0029 | 0.0047 | 0.0059 | 0.0024 | 0.0032 | 0.0019 | 0.0026 |
Vacuole Periphery | 0.0011 | 0.0005 | 0.0007 | 0.0008 | 0.0031 | 0.0006 | 0.0024 | 0.0008 | 0.0002 | 0.0007 | 0.0014 | 0.0002 | 0.0019 | 0.0006 | 0.0004 | 0.0003 | 0.0001 | 0.0004 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 49.0816 | 25.548 | 21.4305 | 27.7969 | 27.3878 | 29.8052 | 24.4277 | 32.5951 | 31.4568 | 36.598 |
Translational Efficiency | 1.1898 | 1.1985 | 1.206 | 1.3114 | 1.2456 | 1.6587 | 1.2978 | 0.8602 | 0.9433 | 1.1579 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1697 | 527 | 1243 | 1706 | 1364 | 1827 | 1847 | 1849 | 3061 | 2354 | 3090 | 3555 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 795.10 | 956.55 | 1085.19 | 1159.25 | 841.13 | 988.78 | 1074.49 | 1074.38 | 815.61 | 981.56 | 1078.79 | 1115.11 |
Standard Deviation | 137.20 | 148.67 | 149.12 | 178.43 | 118.07 | 132.80 | 141.12 | 162.13 | 131.04 | 137.17 | 144.49 | 175.35 |
Intensity Change Log 2 | — | 0.266704 | 0.448739 | 0.543984 | — | 0.233321 | 0.353251 | 0.353104 | — | 0.249639 | 0.400442 | 0.449015 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000991 | 0.001881 | 0.002893 | 0.008957 | 0.000729 | 0.000717 | 0.004135 | 0.003051 | 0.000874 | 0.000977 | 0.003635 | 0.005885 |
Bud Neck | 0.002940 | 0.001863 | 0.001967 | 0.008473 | 0.001377 | 0.002111 | 0.002384 | 0.012279 | 0.002243 | 0.002056 | 0.002216 | 0.010452 |
Bud Site | 0.006851 | 0.008506 | 0.008258 | 0.031703 | 0.004347 | 0.003646 | 0.008723 | 0.023595 | 0.005735 | 0.004734 | 0.008536 | 0.027486 |
Cell Periphery | 0.001450 | 0.001637 | 0.001226 | 0.005798 | 0.001114 | 0.002096 | 0.001713 | 0.000913 | 0.001300 | 0.001993 | 0.001517 | 0.003257 |
Cytoplasm | 0.141508* | 0.120175* | 0.079192 | 0.128549* | 0.121036* | 0.164974* | 0.093843 | 0.194430* | 0.132385* | 0.154945* | 0.087949 | 0.162814* |
Cytoplasmic Foci | 0.058391 | 0.049169 | 0.008817 | 0.024295 | 0.037751 | 0.028441 | 0.011566 | 0.067702 | 0.049194 | 0.033081 | 0.010460 | 0.046871 |
Eisosomes | 0.000608 | 0.000398 | 0.000215 | 0.000901 | 0.000575 | 0.001037 | 0.000661 | 0.000302 | 0.000594 | 0.000894 | 0.000482 | 0.000589 |
Endoplasmic Reticulum | 0.601222* | 0.702208* | 0.810262* | 0.563468* | 0.731563* | 0.682498* | 0.791667* | 0.318998* | 0.659303* | 0.686911* | 0.799147* | 0.436316* |
Endosome | 0.015337 | 0.016687 | 0.008452 | 0.019847 | 0.009517 | 0.007642 | 0.007357 | 0.035849 | 0.012743 | 0.009667 | 0.007798 | 0.028170 |
Golgi | 0.028712 | 0.016422 | 0.002763 | 0.008115 | 0.020985 | 0.021239 | 0.004058 | 0.006030 | 0.025269 | 0.020160 | 0.003537 | 0.007031 |
Lipid Particles | 0.058500 | 0.025659 | 0.010782 | 0.032365 | 0.029334 | 0.036965 | 0.015247 | 0.052688 | 0.045504 | 0.034434 | 0.013451 | 0.042935 |
Mitochondria | 0.013551 | 0.003167 | 0.000409 | 0.002258 | 0.008757 | 0.008180 | 0.001734 | 0.004086 | 0.011415 | 0.007058 | 0.001201 | 0.003209 |
Mitotic Spindle | 0.000370 | 0.000833 | 0.000657 | 0.007781 | 0.000117 | 0.000065 | 0.000845 | 0.007805 | 0.000258 | 0.000237 | 0.000770 | 0.007794 |
None | 0.013461 | 0.001942 | 0.000486 | 0.000948 | 0.004823 | 0.009684 | 0.001398 | 0.001998 | 0.009612 | 0.007951 | 0.001031 | 0.001494 |
Nuclear Periphery | 0.004548 | 0.002290 | 0.003645 | 0.001505 | 0.000903 | 0.001292 | 0.002893 | 0.009643 | 0.002924 | 0.001515 | 0.003195 | 0.005737 |
Nuclear Periphery Foci | 0.010554 | 0.010384 | 0.028151 | 0.017742 | 0.004531 | 0.003344 | 0.012245 | 0.031916 | 0.007870 | 0.004920 | 0.018644 | 0.025114 |
Nucleolus | 0.001325 | 0.000156 | 0.000043 | 0.000484 | 0.000312 | 0.000205 | 0.000063 | 0.001118 | 0.000874 | 0.000194 | 0.000055 | 0.000814 |
Nucleus | 0.015958 | 0.007473 | 0.011589 | 0.014639 | 0.006715 | 0.009941 | 0.012821 | 0.046879 | 0.011840 | 0.009388 | 0.012326 | 0.031408 |
Peroxisomes | 0.004927 | 0.002378 | 0.000483 | 0.001359 | 0.002695 | 0.004806 | 0.001222 | 0.003018 | 0.003932 | 0.004262 | 0.000925 | 0.002222 |
Vacuole | 0.014888 | 0.022524 | 0.019170 | 0.116480 | 0.010079 | 0.009601 | 0.024566 | 0.174382 | 0.012745 | 0.012495 | 0.022395 | 0.146595 |
Vacuole Periphery | 0.003907 | 0.004246 | 0.000542 | 0.004333 | 0.002739 | 0.001515 | 0.000860 | 0.003320 | 0.003387 | 0.002127 | 0.000732 | 0.003806 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -1.92 | -3.06 | -6.71 | -5.75 | -4.63 | 0.05 | -5.53 | -5.50 | -5.64 | 1.62 | -0.59 | -6.14 | -8.12 | -7.97 | -3.04 |
Bud Neck | 1.81 | 1.58 | -6.63 | -7.95 | -7.96 | -1.46 | -2.55 | -10.46 | -9.42 | -9.26 | 0.44 | -0.25 | -12.02 | -11.91 | -12.11 |
Bud Site | -0.95 | -1.17 | -10.32 | -8.90 | -9.08 | 0.68 | -3.81 | -9.46 | -9.76 | -6.54 | 1.09 | -3.19 | -13.76 | -14.24 | -11.15 |
Cell Periphery | -0.29 | 0.91 | -6.13 | -4.30 | -6.60 | -3.28 | -2.98 | 1.54 | 4.26 | 4.55 | -2.50 | -1.39 | -5.34 | -2.61 | -4.68 |
Cytoplasm | 2.34 | 9.76 | 0.24 | -2.13 | -9.11 | -7.18 | 4.50 | -11.44 | -5.24 | -16.25 | -4.70 | 10.27 | -8.34 | -3.21 | -17.92 |
Cytoplasmic Foci | 1.82 | 16.97 | 10.75 | 5.04 | -8.38 | 3.11 | 10.03 | -8.69 | -12.65 | -20.40 | 6.45 | 19.42 | 0.77 | -6.17 | -21.09 |
Eisosomes | 2.17 | 5.11 | -1.49 | -2.79 | -4.22 | -2.84 | -0.57 | 2.49 | 6.11 | 3.36 | -2.68 | 1.21 | 0.40 | 2.89 | -0.74 |
Endoplasmic Reticulum | -5.97 | -17.05 | 6.79 | 10.97 | 23.32 | 4.44 | -4.89 | 36.70* | 34.45* | 44.76* | -3.09 | -16.33 | 28.27 | 30.74 | 46.44* |
Endosome | -0.74 | 5.90 | -2.65 | -1.24 | -8.05 | 2.38 | 2.77 | -16.66 | -18.05 | -18.49 | 3.94 | 7.04 | -13.62 | -16.58 | -19.52 |
Golgi | 4.75 | 14.93 | 10.93 | 4.15 | -4.22 | -0.13 | 10.90 | 9.79 | 11.97 | -2.60 | 3.28 | 18.29 | 14.84 | 11.70 | -4.77 |
Lipid Particles | 9.10 | 16.48 | 8.22 | -2.09 | -10.14 | -2.73 | 6.20 | -7.15 | -4.53 | -13.44 | 4.63 | 16.63 | 1.57 | -3.34 | -16.43 |
Mitochondria | 9.22 | 12.83 | 10.65 | 1.85 | -5.08 | 0.43 | 6.27 | 4.00 | 4.45 | -3.44 | 4.47 | 13.36 | 10.27 | 5.71 | -4.94 |
Mitotic Spindle | -0.72 | -1.18 | -5.65 | -4.86 | -5.31 | 0.99 | -2.19 | -7.19 | -7.24 | -6.31 | 0.13 | -2.28 | -8.99 | -8.93 | -8.16 |
None | 6.38 | 7.68 | 7.31 | 1.39 | -1.06 | -3.20 | 3.72 | 2.84 | 5.78 | -0.85 | 1.21 | 8.33 | 7.69 | 6.37 | -1.05 |
Nuclear Periphery | 2.19 | 1.08 | 3.78 | 0.96 | 5.25 | -0.92 | -4.13 | -8.79 | -7.80 | -6.07 | 2.56 | -0.54 | -4.05 | -6.64 | -4.03 |
Nuclear Periphery Foci | 0.26 | -8.00 | -4.95 | -4.32 | 4.15 | 1.96 | -6.49 | -12.89 | -13.71 | -8.21 | 4.03 | -8.95 | -12.67 | -15.95 | -4.10 |
Nucleolus | 6.05 | 6.67 | 2.35 | -1.33 | -1.71 | 0.67 | 1.53 | -3.36 | -4.77 | -5.47 | 5.29 | 6.37 | 0.07 | -3.90 | -4.69 |
Nucleus | 5.25 | 2.21 | -1.38 | -7.16 | -3.86 | -3.67 | -5.45 | -17.85 | -16.49 | -14.03 | 2.71 | -1.05 | -14.64 | -17.44 | -13.78 |
Peroxisomes | 5.27 | 11.89 | 9.48 | 3.07 | -5.46 | -3.92 | 5.20 | -0.82 | 3.43 | -6.00 | -0.73 | 11.97 | 6.82 | 5.09 | -7.13 |
Vacuole | -2.72 | -2.97 | -23.87 | -20.84 | -22.06 | 0.35 | -7.71 | -32.27 | -32.41 | -27.72 | 0.18 | -7.60 | -39.61 | -39.33 | -34.97 |
Vacuole Periphery | -0.36 | 7.39 | -0.01 | 0.30 | -4.55 | 4.63 | 5.42 | -1.08 | -5.19 | -5.91 | 3.95 | 8.92 | -0.28 | -3.25 | -6.78 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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