Standard name
Human Ortholog
Description Cytosolic protein required for sporulation
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Cytoplasm 0.85 0.92 0.97 0.98 0.97 0.95 0.91 – 0.78 0.75 0.61 0.7 0.96 0.97 0.99 0.9 0.85 0.88 0.47 0.57 0.59 0.66 0.79 0.78
Endoplasmic Reticulum 0.06 0.07 0 0 0 0 0 – 0 0 0 0 0 0 0 0.09 0.07 0.08 0.24 0.12 0 0.09 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.12 0 0 0 0 0.05 0.08 – 0.25 0.25 0.51 0.32 0 0 0 0 0 0.07 0.14 0.15 0.2 0.12 0.08 0.07
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0.08 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 3 – 0 2 0 2 0 0 0 3 4 0 1 3 2 0 1 1
Bud 1 1 1 1 0 3 5 – 7 7 7 7 2 4 0 2 2 0 0 0 0 0 1 1
Bud Neck 0 0 0 2 4 7 8 – 5 10 4 4 0 1 2 3 5 1 2 3 2 0 0 0
Bud Site 0 0 0 0 0 0 1 – 3 1 1 2 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 2 0 2 4 2 0 – 1 1 0 3 0 1 1 6 10 3 0 0 0 0 0 0
Cytoplasm 55 89 70 140 271 221 359 – 155 157 192 248 53 116 196 181 162 123 47 67 59 14 63 106
Endoplasmic Reticulum 4 7 0 2 1 1 0 – 0 7 1 6 1 0 1 19 13 11 24 14 3 2 2 2
Endosome 0 0 0 0 1 0 2 – 3 0 0 1 0 0 0 1 1 3 1 2 0 0 0 2
Golgi 0 0 0 0 1 0 0 – 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0
Mitochondria 8 0 0 0 1 11 31 – 49 52 161 113 0 4 0 8 6 10 14 17 20 2 6 10
Nucleus 0 0 2 0 2 0 2 – 1 2 3 2 0 0 0 1 1 1 0 0 0 0 0 1
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 2 0 0 0 0 1 0 0 1 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 3 1 7 – 3 2 10 7 0 0 0 4 3 1 0 0 0 0 0 1
Vac/Vac Membrane 1 1 0 0 3 4 5 – 3 1 5 8 0 0 0 2 3 2 5 5 8 0 0 3
Unique Cell Count 65 97 72 143 280 232 395 198 208 314 353 55 120 197 202 191 139 101 119 101 22 81 136
Labelled Cell Count 69 100 73 147 291 250 423 232 242 384 403 56 127 200 231 211 155 101 119 101 22 81 136


Ambiguous

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.0 5.3 5.5 5.3 5.8 4.4 4.5 – 4.1 3.9 3.6 4.1 4.9 5.2 5.5 5.8 5.6 5.5 – 5.3 5.6
Std Deviation (1e-4) 0.4 0.7 0.9 1.3 2.0 0.8 1.0 – 2.1 0.6 1.2 1.5 0.9 1.2 1.3 1.1 1.1 1.3 – 0.9 1.4
Intensity Change (Log2) – – – -0.05 0.08 -0.32 -0.28 – -0.43 -0.49 -0.6 -0.44 -0.16 -0.07 -0.01 0.08 0.04 0.01 – -0.05 0.02


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.3 -0.2 -0.7 -1.8 – -3.7 -4.1 -5.9 -4.8 -0.3 -0.2 1.6 -2.0 -2.8 -2.2
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.7325 -0.4187 -0.4765 -0.765 -0.9937 -0.7739 0.2129 0.4109 0.4676 0.0441 0.7479 0.3768 -0.5556 -0.3704 -0.7101 -0.7212 -0.5326 -0.7371
Actin 0.035 0.0032 0.0213 0.0294 0.0006 0.0019 0.0451 0.0009 0.0126 0.0087 0.0259 0.0097 0.0558 0.0035 0.0169 0.0106 0.0637 0.0062
Bud 0.0011 0 0.0003 0.0004 0 0.0001 0.0006 0.0005 0.0014 0.0005 0.0084 0.0041 0.0014 0.0004 0.0007 0.0047 0.0004 0.0001
Bud Neck 0.0054 0.0001 0.0005 0.0016 0.0001 0.0029 0.0008 0.001 0.0014 0.0009 0.0014 0.0014 0.0078 0.0002 0.0011 0.0008 0.0012 0.0009
Bud Periphery 0.0018 0.0001 0.0005 0.0015 0.0001 0.0002 0.0011 0.0008 0.0037 0.0009 0.0277 0.0053 0.0023 0.0006 0.002 0.0098 0.0008 0.0003
Bud Site 0.0498 0.0007 0.002 0.0008 0 0.0001 0.0028 0.0181 0.0175 0.0009 0.0025 0.0005 0.0214 0.0036 0.0063 0.0042 0.0004 0.0003
Cell Periphery 0.0037 0.0009 0.0011 0.0017 0.0006 0.0004 0.0006 0.0014 0.0016 0.0004 0.0005 0.0007 0.0018 0.0005 0.0008 0.0008 0.0003 0.0001
Cytoplasm 0.1978 0.318 0.3289 0.1863 0.3057 0.3623 0.2232 0.3967 0.3393 0.3179 0.3476 0.3493 0.1597 0.2369 0.2947 0.2693 0.3146 0.4003
Cytoplasmic Foci 0.0496 0.042 0.0367 0.0243 0.0031 0.0453 0.0385 0.014 0.0251 0.0331 0.0323 0.0235 0.0395 0.0386 0.0544 0.0696 0.026 0.0267
Eisosomes 0.002 0.0003 0.001 0.0013 0.0001 0.0005 0.0004 0.0002 0.0004 0.0003 0.0002 0.0002 0.001 0.0003 0.0005 0.0002 0.0009 0.0001
Endoplasmic Reticulum 0.0211 0.0253 0.0362 0.0152 0.0058 0.0171 0.0326 0.0227 0.0428 0.0306 0.0088 0.0163 0.0291 0.02 0.025 0.0345 0.0276 0.0116
Endosome 0.025 0.0073 0.0244 0.0471 0.0006 0.0079 0.0302 0.0093 0.0257 0.0491 0.038 0.0237 0.0236 0.0189 0.0506 0.046 0.0456 0.0232
Golgi 0.0134 0.0011 0.0187 0.0024 0.0002 0.0024 0.0112 0.001 0.0052 0.0221 0.0285 0.0057 0.0075 0.0017 0.0054 0.0057 0.0198 0.0045
Lipid Particles 0.0503 0.018 0.0151 0.0132 0.0007 0.0285 0.0209 0.0029 0.0075 0.0061 0.0229 0.0058 0.0202 0.0107 0.0181 0.0235 0.0446 0.0089
Mitochondria 0.0296 0.0003 0.0312 0.0019 0.1239 0.0117 0.0033 0.0113 0.0058 0.0329 0.0345 0.0035 0.0034 0.0017 0.0043 0.0167 0.0063 0.0028
None 0.43 0.567 0.4644 0.64 0.5568 0.4775 0.5454 0.5079 0.4889 0.4783 0.3963 0.5332 0.5811 0.647 0.4825 0.4492 0.377 0.4955
Nuclear Periphery 0.0032 0.0018 0.0043 0.0091 0.0002 0.0179 0.0043 0.0017 0.0027 0.0026 0.0014 0.0012 0.004 0.0016 0.0028 0.0117 0.0253 0.0091
Nucleolus 0.002 0.0002 0.0004 0.0011 0 0.0004 0.0007 0.0006 0.0008 0.0002 0.0003 0.0002 0.0012 0.001 0.0025 0.0012 0.0006 0.0002
Nucleus 0.0021 0.0008 0.0011 0.0048 0.0002 0.0055 0.0016 0.0027 0.0019 0.0013 0.0014 0.0013 0.0019 0.0011 0.0028 0.0035 0.0024 0.0012
Peroxisomes 0.0338 0.0088 0.0051 0.0015 0.0003 0.0102 0.0227 0.0005 0.0073 0.0038 0.0167 0.0105 0.0286 0.0038 0.0188 0.0245 0.0267 0.0031
Punctate Nuclear 0.0379 0.0018 0.0015 0.0037 0.0001 0.0017 0.0096 0.0013 0.002 0.001 0.001 0.0015 0.0031 0.0044 0.0045 0.0066 0.0082 0.0025
Vacuole 0.0045 0.0021 0.0045 0.0104 0.0007 0.003 0.0036 0.0041 0.006 0.0048 0.0027 0.002 0.005 0.0033 0.0044 0.0052 0.0045 0.0014
Vacuole Periphery 0.0009 0.0001 0.0007 0.0024 0.0001 0.0027 0.0008 0.0003 0.0006 0.0034 0.0009 0.0005 0.0005 0.0003 0.0007 0.0016 0.0033 0.0008

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 11.9272 7.9346 9.1998 12.2356 12.9467 8.8711 10.6552 12.1833 9.8927 14.6582
Translational Efficiency 0.7308 0.8977 0.7896 0.6453 0.717 1.1257 0.9071 0.5625 0.8495 0.7372

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2192 979 995 1517 2206 1304 1396 2033 4398 2283 2391 3550

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 626.39 711.16 855.17 897.69 705.29 650.11 888.87 904.79 665.97 676.29 874.85 901.76
Standard Deviation 63.88 105.49 101.09 120.32 79.56 96.10 97.58 115.07 82.25 104.69 100.44 117.39
Intensity Change Log 2 — 0.183113 0.449150 0.519156 — -0.117533 0.333756 0.359366 — 0.031706 0.389186 0.436736

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000210 0.000384 0.000658 0.003435 0.000258 0.000655 0.000274 0.003035 0.000234 0.000539 0.000434 0.003206
Bud Neck 0.020821 0.016483 0.006219 0.014104 0.014951 0.026553 0.003228 0.029859 0.017876 0.022235 0.004472 0.023127
Bud Site 0.008024 0.010850 0.009638 0.053150 0.005900 0.016454 0.004712 0.033966 0.006959 0.014051 0.006762 0.042164
Cell Periphery 0.000291 0.000164 0.000403 0.000877 0.000233 0.000251 0.000235 0.000551 0.000262 0.000214 0.000305 0.000690
Cytoplasm 0.438604* 0.443855* 0.412121* 0.392109* 0.474012* 0.360020* 0.560187* 0.437028* 0.456364* 0.395970* 0.498571* 0.417833*
Cytoplasmic Foci 0.238469* 0.256742* 0.031444 0.109765 0.231875* 0.333548* 0.081656 0.071165 0.235162* 0.300612* 0.060761 0.087660
Eisosomes 0.000434 0.000158 0.000078 0.000732 0.000385 0.000440 0.000102 0.000563 0.000409 0.000319 0.000092 0.000635
Endoplasmic Reticulum 0.007900 0.004163 0.011278 0.031541 0.004245 0.003815 0.006113 0.021387 0.006067 0.003964 0.008262 0.025726
Endosome 0.013697 0.028111 0.004068 0.016764 0.011651 0.042017 0.009154 0.011122 0.012670 0.036054 0.007037 0.013533
Golgi 0.006555 0.005191 0.000128 0.007100 0.004813 0.011045 0.000171 0.002438 0.005681 0.008534 0.000153 0.004431
Lipid Particles 0.028854 0.015841 0.003259 0.031813 0.016471 0.033449 0.007474 0.029464 0.022643 0.025899 0.005720 0.030468
Mitochondria 0.010465 0.006465 0.000436 0.001897 0.004325 0.012722 0.000208 0.003152 0.007385 0.010039 0.000303 0.002615
Mitotic Spindle 0.000370 0.003566 0.001385 0.016003 0.003219 0.004252 0.001601 0.006558 0.001799 0.003958 0.001511 0.010594
None 0.008709 0.006208 0.002571 0.001212 0.003357 0.003358 0.002750 0.001582 0.006024 0.004580 0.002675 0.001424
Nuclear Periphery 0.000884 0.001508 0.002134 0.001766 0.000565 0.000696 0.002057 0.001853 0.000724 0.001045 0.002089 0.001816
Nuclear Periphery Foci 0.000869 0.002771 0.007377 0.018074 0.000675 0.001474 0.013293 0.006871 0.000771 0.002030 0.010831 0.011658
Nucleolus 0.001814 0.002952 0.000399 0.000507 0.001043 0.003457 0.000528 0.000912 0.001427 0.003240 0.000474 0.000739
Nucleus 0.148823* 0.091620 0.421691* 0.118432 0.156827* 0.052906 0.172043* 0.210826* 0.152838* 0.069507 0.275932* 0.171344*
Peroxisomes 0.005019 0.007034 0.000699 0.004225 0.003793 0.015037 0.000967 0.005396 0.004404 0.011605 0.000856 0.004896
Vacuole 0.057597 0.093196 0.083827 0.174890 0.060210 0.074969 0.133069 0.121677 0.058908 0.082785 0.112577 0.144416
Vacuole Periphery 0.001593 0.002738 0.000186 0.001604 0.001193 0.002883 0.000179 0.000595 0.001392 0.002821 0.000182 0.001026

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.96 -7.40 -7.88 -7.31 -6.25 -4.16 -0.43 -6.10 -5.09 -6.04 -5.23 -5.13 -9.44 -8.26 -8.51
Bud Neck 2.56 9.71 5.27 1.56 -5.09 -5.40 11.42 -6.89 -0.73 -14.66 -2.85 15.44 -3.18 0.06 -15.17
Bud Site -1.84 -1.33 -11.17 -10.17 -10.02 -5.35 1.21 -10.18 -4.75 -10.62 -5.43 -0.05 -14.98 -10.38 -14.53
Cell Periphery 7.35 -4.98 -9.48 -12.08 -6.71 -0.61 0.33 -9.76 -6.86 -9.76 2.88 -3.52 -13.05 -13.50 -10.99
Cytoplasm -0.26 5.79 7.61 5.97 0.75 12.88 -5.63 7.13 -6.48 11.31 9.16 -1.28 9.90 -0.81 9.07
Cytoplasmic Foci -2.33 45.31 26.20 21.14 -18.69 -13.94 29.62 33.57 40.92 3.17 -12.13 49.65 42.94 44.17 -8.38
Eisosomes 11.90 15.95 -1.36 -3.58 -4.14 -1.39 11.35 -2.89 -1.53 -11.65 3.91 18.94 -2.36 -3.90 -7.90
Endoplasmic Reticulum 4.08 -4.98 -9.15 -11.20 -6.62 0.60 -3.00 -10.07 -10.34 -8.99 3.56 -5.05 -13.31 -15.21 -11.05
Endosome -6.96 12.74 -0.42 6.43 -10.22 -14.11 2.15 1.14 14.54 -0.94 -15.38 8.31 0.78 15.45 -6.65
Golgi 1.50 13.67 1.03 -0.21 -4.97 -5.76 10.46 5.50 8.92 -7.88 -3.71 17.09 3.89 6.08 -7.03
Lipid Particles 7.19 18.46 0.87 -4.82 -11.97 -8.03 8.73 -5.51 2.36 -10.94 -2.20 19.21 -2.90 -0.68 -15.96
Mitochondria 2.68 10.02 8.04 3.63 -2.32 -5.29 6.49 2.19 6.68 -7.03 -2.23 11.94 7.82 7.57 -6.62
Mitotic Spindle -2.57 -1.57 -6.35 -4.28 -5.46 -0.77 1.26 -2.20 -1.29 -3.69 -2.33 0.06 -6.54 -4.13 -6.58
None 1.63 6.57 7.81 3.85 4.43 -0.10 1.50 4.08 3.26 1.79 1.77 6.18 8.71 4.97 3.19
Nuclear Periphery -0.97 -8.07 -5.46 -0.65 2.12 -1.04 -11.99 -9.04 -6.95 0.59 -1.12 -13.62 -10.06 -3.37 1.67
Nuclear Periphery Foci -5.10 -10.98 -15.09 -12.47 -7.34 -3.03 -12.14 -11.06 -8.88 5.28 -5.73 -15.39 -18.49 -15.16 -0.15
Nucleolus -2.01 8.40 7.91 4.35 -1.49 -2.99 2.96 -0.26 2.92 -2.68 -3.48 8.09 4.84 4.62 -2.93
Nucleus 11.43 -30.14 6.16 -5.31 32.70 27.64 -2.77 -9.72 -32.13 -6.02 27.39 -22.42 -4.84 -27.51 17.93
Peroxisomes -3.00 11.03 2.95 5.09 -6.27 -9.62 9.51 -2.11 8.07 -7.59 -9.81 14.50 0.24 9.36 -9.77
Vacuole -8.62 -9.65 -26.92 -17.87 -14.43 -4.34 -18.77 -20.48 -15.30 2.12 -9.18 -20.81 -33.22 -23.23 -7.56
Vacuole Periphery -3.26 15.20 0.88 2.85 -2.76 -5.72 7.91 4.48 8.46 -3.64 -6.39 15.13 3.02 7.36 -3.77
Standard name
Human Ortholog
Description Cytosolic protein required for sporulation
Localization
Cell Percentages cytoplasm (28%), ER (9%), mixed (54%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Rmd8

Rmd8


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rmd8-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available