Standard name
Human Ortholog
Description Subunit of the mitochondrial alpha-ketoglutarate dehydrogenase; recruits E3 subunit (Lpd1p) to the E1-E2 (Kgd1p, Kgd2p) core; has similarity to human mitochondrial ribosomal protein MRP-S36
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Bud 0 0 0.06 0.12 0.06 0.05 – 0.06 0.05 0.09 0 0.07 0.05 0 0 0 – 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Bud Site 0 0 0 0.07 0 0 – 0 0 0 0 0 0 0 0 0 – 0 – – – – – –
Cell Periphery 0 0.05 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – 0.1 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Endosome 0.06 0 0 0 0.06 0 – 0 0 0 0 0 0.18 0.15 0.16 0 – 0.06 0.07 0 0.07 0.12 0.14 0.11
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.05 0.25 – 0.12 0.07 0.08 0 0.07 0.07 0.06
Mitochondria 0.85 0.87 0.96 0.2 0.23 0.55 – 0.69 0.74 0.71 0.74 0.77 0.5 0.69 0.61 0.15 – 0.1 0.69 0.74 0.72 0.28 0.24 0.26
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Nucleolus 0.05 0 0.05 0.07 0.11 0.19 – 0.15 0.14 0.16 0.15 0.1 0 0 0 0 – 0.08 0 0 0 0 0 0
Peroxisomes 0.07 0.05 0 0.67 0.45 0.27 – 0.19 0.16 0.15 0.13 0.16 0.46 0.26 0.3 0.41 – 0.35 0.05 0.05 0.12 0.3 0.33 0.3
SpindlePole 0 0 0 0.47 0.46 0.21 – 0.15 0.15 0.14 0.11 0.09 0.23 0.08 0.1 0.18 – 0.29 0 0 0 0.16 0.13 0.17
Vac/Vac Membrane 0.14 0.13 0 0 0 0 – 0 0 0 0 0 0 0.07 0.07 0 – 0.2 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 2 1 1 1 1 0 – 0 0 1 1 1 6 0 1 2 – 0 19 13 3 13 22 23
Bud 13 2 22 33 21 22 – 29 20 41 18 36 32 11 12 6 – 2 3 2 0 3 4 6
Bud Neck 1 0 0 3 8 8 – 1 3 4 1 2 3 1 0 0 – 0 1 0 1 1 4 2
Bud Site 5 0 8 20 3 16 – 7 9 11 16 17 2 1 1 0 – 0 – – – – – –
Cell Periphery 14 18 10 0 0 0 – 1 1 4 1 4 10 5 7 3 – 0 0 0 0 0 0 1
Cytoplasm 6 3 6 6 6 10 – 8 3 7 7 5 7 7 7 4 – 5 0 0 1 0 0 0
Endoplasmic Reticulum 0 0 1 1 0 0 – 0 0 0 0 0 1 0 0 2 – 1 0 0 1 0 0 1
Endosome 52 7 6 0 19 7 – 8 1 4 1 2 108 75 86 4 – 3 61 13 23 56 89 77
Golgi 8 14 0 0 0 0 – 0 0 0 0 0 3 21 26 34 – 6 60 26 14 33 44 39
Mitochondria 693 293 340 56 79 258 – 361 280 331 304 384 303 355 335 21 – 5 582 260 258 131 151 176
Nucleus 5 2 0 0 2 4 – 5 3 1 0 3 3 2 1 2 – 0 1 1 0 0 1 0
Nuclear Periphery 25 13 10 0 0 7 – 8 10 10 7 10 3 7 5 0 – 0 8 4 2 1 1 3
Nucleolus 44 9 17 20 39 90 – 79 55 77 64 50 26 21 19 6 – 4 4 1 0 2 4 4
Peroxisomes 61 17 14 184 155 128 – 101 62 70 53 79 281 135 163 56 – 17 42 18 43 140 208 199
SpindlePole 27 2 10 130 157 100 – 79 57 64 45 44 139 40 53 25 – 14 17 1 3 75 86 117
Vac/Vac Membrane 114 43 14 0 3 10 – 9 5 7 4 7 25 34 39 6 – 10 37 8 1 5 7 5
Unique Cell Count 815 335 353 274 344 469 522 380 469 413 498 608 511 546 138 49 850 354 359 475 640 675
Labelled Cell Count 1070 424 459 454 493 660 696 509 632 522 644 952 715 755 171 67 850 354 359 475 640 675


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.9 9.7 5.4 6.8 8.1 7.5 – 8.5 8.4 9.0 9.5 8.9 8.3 9.2 9.3 17.9 – 15.8 9.2 9.4 9.1
Std Deviation (1e-4) 1.7 2.0 1.2 1.9 2.2 2.6 – 2.7 3.1 3.4 3.9 3.7 1.8 2.1 2.4 5.5 – 4.6 5.8 3.8 2.1
Intensity Change (Log2) – – – 0.32 0.57 0.46 – 0.64 0.62 0.73 0.81 0.72 0.6 0.76 0.77 1.72 – 1.54 0.75 0.79 0.74


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 – 0 0 0 0 0 0 0 0 0 – 0
Bud 2.6 -0.1 -1.0 – -0.4 -0.6 1.3 -1.2 0.6 -0.6 -3.1 -3.1 -0.8 – 0
Bud Neck 0 0 0 – 0 0 0 0 0 0 0 0 0 – 0
Bud Site 3.0 -1.5 1.0 – -1.0 0.1 0.1 1.3 1.0 0 0 0 0 – 0
Cell Periphery 0 0 0 – 0 -2.9 -2.2 -3.0 -2.3 -1.2 -2.1 -1.7 0 – 0
Cytoplasm 0.4 0 0.4 – -0.2 0 -0.2 0 0 0 -0.4 -0.5 0 – 0
Endoplasmic Reticulum 0 0 0 – 0 0 0 0 0 0 0 0 0 – 0
Endosome 0 2.7 -0.2 – -0.2 0 0 0 0 7.4 6.4 6.8 0 – 0
Golgi 0 0 0 – 0 0 0 0 0 0 3.9 4.2 9.7 – 0
Mitochondria -19.5 -19.8 -13.2 – -9.9 -8.5 -9.4 -8.6 -7.7 -14.8 -9.8 -11.8 -18.3 – -16.2
Nucleus 0 0 0 – 0 0 0 0 0 0 0 0 0 – 0
Nuclear Periphery 0 0 -1.3 – -1.3 -0.2 -0.6 -1.1 -0.8 0 -1.5 -2.2 0 – 0
Nucleolus 1.3 3.2 6.1 – 4.8 4.4 5.2 4.8 2.8 -0.4 -0.5 -1.0 -0.2 – 0
Peroxisomes 16.9 12.7 8.8 – 6.6 5.5 5.1 4.3 5.5 13.7 8.6 9.5 10.4 – 0
SpindlePole 13.3 13.2 7.7 – 5.9 5.7 5.4 4.3 3.5 8.3 3.1 3.9 5.9 – 0
Vacuole -3.3 -2.6 -1.5 – -2.0 -2.3 -2.2 -2.7 -2.4 0.1 1.7 2.0 0.2 – 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 6.6392 7.8841 7.205 5.6696 4.9692 7.0937 5.6625 6.6069 6.3688 4.9468 4.586 6.2158 7.8234 7.7957 6.8317 6.5655 5.1802 7.5173
Actin 0.0044 0.0065 0.0201 0.0355 0.029 0.0034 0.028 0.0112 0.0197 0.0009 0.0049 0.0135 0.0629 0.0044 0.0071 0.0002 0.0011 0.0513
Bud 0.0003 0.0026 0.0011 0.0059 0.0011 0.0001 0.0004 0.0024 0.0008 0.0001 0.0006 0.0002 0.0003 0.0017 0.0008 0.0002 0.0005 0.0002
Bud Neck 0.0016 0.001 0.0006 0.0026 0.0016 0.0007 0.0029 0.0013 0.0072 0.0004 0.0012 0.0023 0.0012 0.0015 0.0014 0.0003 0.001 0.0028
Bud Periphery 0.0004 0.002 0.001 0.0034 0.0014 0.0001 0.0005 0.0028 0.0008 0.0001 0.001 0.0003 0.0003 0.0008 0.0017 0.0002 0.0002 0.0002
Bud Site 0.0023 0.0161 0.0078 0.0132 0.0079 0.0004 0.0035 0.0164 0.0104 0.0003 0.0049 0.001 0.0012 0.0143 0.0021 0.0015 0.0005 0.0008
Cell Periphery 0.0005 0.0004 0.0003 0.0012 0.0003 0.0001 0.0005 0.0005 0.0003 0.0001 0.0004 0.0001 0.0001 0.0005 0.0002 0 0.0002 0.0001
Cytoplasm 0.0011 0.0004 0.0003 0.0037 0.0008 0.0002 0.0034 0.0003 0.0004 0.0001 0.0005 0.0001 0.0034 0.0002 0.0001 0.0001 0.0012 0.0031
Cytoplasmic Foci 0.2657 0.3584 0.3469 0.3246 0.3895 0.3248 0.3309 0.401 0.3635 0.4889 0.3993 0.3549 0.1642 0.3533 0.3891 0.3269 0.4117 0.3271
Eisosomes 0.0001 0.0001 0.0002 0.0002 0.0003 0.0001 0.002 0.0001 0.0002 0 0.0002 0.0002 0.0003 0.0001 0.0001 0 0.0001 0.0003
Endoplasmic Reticulum 0.0005 0.0001 0.0002 0.0008 0.0009 0.0005 0.0011 0 0.0003 0 0.0008 0 0.0012 0 0 0 0 0.0022
Endosome 0.0201 0.0161 0.0144 0.0189 0.0335 0.0138 0.0197 0.0085 0.0222 0.0077 0.0387 0.0089 0.0098 0.0091 0.0184 0.0068 0.0046 0.0284
Golgi 0.0245 0.0346 0.0163 0.0306 0.033 0.0258 0.0285 0.0152 0.0284 0.0166 0.0325 0.0169 0.0207 0.0205 0.0182 0.0091 0.0084 0.0252
Lipid Particles 0.2916 0.2105 0.182 0.246 0.2324 0.3038 0.1565 0.1104 0.1266 0.213 0.2003 0.225 0.2051 0.1618 0.1365 0.2987 0.3089 0.2041
Mitochondria 0.1311 0.097 0.138 0.0831 0.0718 0.1011 0.1462 0.0713 0.1285 0.051 0.0838 0.0632 0.2356 0.1282 0.1128 0.0092 0.038 0.1674
None 0.0004 0.0003 0.0004 0.0003 0.0008 0.0001 0.0041 0.0021 0.0003 0 0.0009 0.0001 0.004 0.0001 0.0001 0.0001 0.0043 0.0011
Nuclear Periphery 0.0059 0.0001 0.0005 0.0008 0.0013 0.0046 0.0015 0.0003 0.0005 0.0001 0.0041 0 0.0142 0.0001 0.0001 0 0 0.0004
Nucleolus 0.0142 0.0076 0.0218 0.0224 0.0271 0.008 0.0106 0.008 0.0154 0.0006 0.0104 0.0083 0.0033 0.0162 0.0203 0.0006 0.0192 0.0008
Nucleus 0.0034 0.0005 0.0016 0.0033 0.0019 0.0008 0.001 0.0005 0.0006 0.0001 0.0019 0.0006 0.006 0.0018 0.001 0 0.0005 0.0001
Peroxisomes 0.2197 0.238 0.2349 0.1928 0.1535 0.2037 0.249 0.3235 0.2614 0.1839 0.1931 0.2909 0.2565 0.272 0.2372 0.3447 0.1839 0.1813
Punctate Nuclear 0.0025 0.0009 0.0051 0.0047 0.0078 0.0038 0.0064 0.0122 0.0079 0.0336 0.0147 0.0128 0.0075 0.0033 0.049 0.0006 0.014 0.0011
Vacuole 0.0046 0.0044 0.0037 0.0041 0.0019 0.0014 0.0017 0.0078 0.0033 0.0008 0.0035 0.0005 0.0007 0.0042 0.0026 0.0003 0.0011 0.0008
Vacuole Periphery 0.005 0.0025 0.0028 0.0018 0.0022 0.0026 0.0017 0.004 0.0013 0.0017 0.0023 0.0004 0.0015 0.0057 0.0012 0.0005 0.0006 0.0012

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 19.2799 10.6062 7.8703 31.8445 24.9973 14.8225 18.0966 26.2375 34.9477 35.639
Translational Efficiency 3.169 4.2032 7.6216 1.9432 2.643 6.735 3.448 3.1254 3.0263 2.4921

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Subunit of the mitochondrial alpha-ketoglutarate dehydrogenase; recruits E3 subunit (Lpd1p) to the E1-E2 (Kgd1p, Kgd2p) core; has similarity to human mitochondrial ribosomal protein MRP-S36
Localization
Cell Percentages mitochondrion (95%)
Cell Cycle Regulation No
Subcompartmental Group mito-2

Ymr31

Ymr31


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ymr31-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available