Standard name
Human Ortholog
Description Subunit of the 19S regulatory particle of the 26S proteasome lid; synthetically lethal with RPT1, which is an ATPase component of the 19S regulatory particle; physically interacts with Nob1p and Rpn3p; protein abundance increases in response to DNA replication stress
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 – 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 – 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 – 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 – 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 – 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0.05 – 0.09 0 0.09 – 0.06 0.15 0.07 0.11 0.08 0.15 0.2 0.07 0.09 0.11 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 – 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 – 0 0 0 – 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 – 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 – 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.81 0.76 0.7 – 0.61 0.47 0.33 – 0.4 0.48 0.43 0.42 0.69 0.55 0.53 0.62 0.61 0.5 0.72 0.76 0.74 0.77 0.58 0.47
Nuclear Periphery 0 0 0 – 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.11 0.1 0 – 0.09 0 0.1 – 0.12 0.11 0.17 0.21 0 0 0 0 0.05 0.06 0.13 0.1 0.19 0.09 0.15 0.14
Peroxisomes 0 0 0 – 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 – 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.05 0.19 0.25 – 0.22 0.47 0.52 – 0.48 0.22 0.31 0.42 0.19 0.26 0.26 0.26 0.23 0.35 0.09 0.07 0.05 0.11 0.19 0.31
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 – 0 0 0 – 0 0 0 0 1 1 1 1 2 1 0 0 0 0 1 0
Bud 0 0 0 – 0 0 0 – 0 0 0 0 0 0 0 0 1 1 1 2 0 4 8 8
Bud Neck 0 0 0 – 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 – 0 0 0 – 1 0 3 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 – 0 0 0 – 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0
Cytoplasm 2 4 1 – 2 2 7 – 8 4 6 2 18 54 78 29 30 30 0 3 0 1 3 3
Endoplasmic Reticulum 0 0 0 – 0 0 0 – 0 0 0 0 0 0 0 10 8 9 0 0 0 0 0 1
Endosome 0 0 0 – 0 0 0 – 0 0 0 1 2 2 1 6 1 1 0 2 0 0 5 3
Golgi 0 0 0 – 0 2 3 – 0 0 0 0 3 5 2 3 10 4 0 0 0 0 1 1
Mitochondria 0 1 0 – 1 0 0 – 1 1 3 0 0 0 2 5 9 4 1 0 0 0 0 4
Nucleus 51 102 14 – 14 22 26 – 54 13 39 8 148 191 205 262 214 135 83 122 41 264 237 224
Nuclear Periphery 0 0 0 – 0 0 0 – 0 0 1 0 1 0 4 6 4 1 0 0 1 0 2 5
Nucleolus 7 13 0 – 2 2 8 – 16 3 15 4 8 15 12 18 17 15 15 16 10 30 60 68
Peroxisomes 0 0 0 – 0 0 0 – 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 – 0 0 0 – 0 0 0 0 1 0 1 0 0 0 0 0 0 1 2 6
Vac/Vac Membrane 3 25 5 – 5 22 41 – 65 6 28 8 40 89 100 108 80 95 10 11 2 37 79 149
Unique Cell Count 63 135 20 23 47 79 135 27 90 19 216 349 389 421 348 269 116 161 56 343 408 480
Labelled Cell Count 63 145 20 24 50 85 145 27 95 23 222 357 408 448 376 296 116 161 56 343 408 480


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 31.3 25.6 36.4 – 19.6 32.8 31.1 – 26.2 23.2 23.0 20.1 31.8 30.3 30.9 25.6 33.5 24.6 33.7 36.2 38.5
Std Deviation (1e-4) 8.0 7.4 8.1 – 7.3 6.7 7.9 – 7.7 8.9 8.8 5.5 10.9 10.3 10.8 9.0 12.3 11.5 9.9 10.2 11.8
Intensity Change (Log2) – – – – -0.89 -0.15 -0.23 – -0.47 -0.65 -0.66 -0.86 -0.19 -0.26 -0.24 -0.51 -0.12 -0.56 -0.11 -0.01 0.08

WT3RAP140RAP220RAP300RAP460RAP540RAP620RAP70002040WT3HU80HU120HU16002040WT3rpd3Δ_1rpd3Δ_2rpd3Δ_302040WT1AF100AF140AF18002040
rapamycinhydroxy-urearpd3 knockoutalpha-factorMultiple Condition Time-Course Intensity PlotsGFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches – 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud – 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck – 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site – 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery – 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cytoplasm – 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum – 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Endosome – 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi – 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria – 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleus – -0.6 -1.7 -3.0 – -2.5 -1.5 -2.2 -1.8 -0.1 -1.3 -1.5 -0.7 -0.8 -1.7
Nuclear Periphery – 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus – 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Peroxisomes – 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole – 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole – 0 1.7 2.2 – 1.9 0 0.5 1.1 0 0.1 0.1 0.1 0 0.9

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 25.2517 31.5093 27.8843 24.4376 28.2358 27.6625 39.0762 43.2994 37.9967 36.9619 37.6701 39.7031 28.0217 35.1046 32.319 33.1472 29.3735 34.343
Actin 0.0068 0 0.0115 0.0105 0.0003 0.0014 0.0006 0.0008 0.0062 0.007 0.0059 0.0001 0.0029 0.0001 0.0051 0 0.005 0.0004
Bud 0.0005 0 0.0001 0.0003 0.0001 0.0001 0.0003 0.0001 0.0002 0.0007 0.0003 0.0001 0.0004 0 0.0002 0 0.0004 0
Bud Neck 0.0009 0.0001 0.0002 0.0002 0.0009 0.0008 0.0001 0.0001 0.0002 0.0004 0.0007 0.0006 0.0003 0.0001 0.0001 0 0.0005 0.0006
Bud Periphery 0.0008 0 0.0002 0.0003 0.0002 0.0003 0.0002 0 0.0002 0.0005 0.0005 0.0001 0.0004 0 0.0002 0 0.0004 0.0001
Bud Site 0.0015 0 0.0003 0.0007 0.0003 0.0001 0.0003 0 0.0005 0.0006 0.0004 0 0.0007 0 0.0004 0 0.0001 0
Cell Periphery 0.0008 0 0.0003 0.0005 0.0011 0.0002 0.0003 0 0.0004 0.0001 0.0007 0.0002 0.0002 0 0 0 0.0001 0.0001
Cytoplasm 0.0099 0.0003 0.0067 0.0063 0.0107 0.0024 0.0045 0.0017 0.0063 0.0072 0.0238 0.0008 0.0171 0.0006 0.0022 0.0001 0.0052 0.0002
Cytoplasmic Foci 0.0139 0 0.0061 0.0212 0.001 0.0004 0.001 0.0003 0.0063 0.0032 0.0166 0.0014 0.0167 0 0.0004 0 0.0004 0
Eisosomes 0.0001 0 0.0001 0.0003 0 0 0 0 0.0001 0 0.0001 0 0 0 0 0 0 0
Endoplasmic Reticulum 0.0044 0 0.0017 0.0021 0.0009 0.0009 0.0004 0.0002 0.0021 0.0009 0.0031 0.0001 0.002 0 0.0002 0 0.0022 0.0001
Endosome 0.0159 0 0.0038 0.0114 0.0005 0.0042 0.0007 0.0025 0.0049 0.0022 0.0088 0.0003 0.0018 0 0.0006 0 0.0047 0.0002
Golgi 0.0026 0 0.0012 0.0019 0 0.0003 0.0001 0.0038 0.0013 0.0006 0.0011 0 0.0002 0 0.0003 0 0.0003 0
Lipid Particles 0.0101 0 0.0033 0.01 0.0009 0.0005 0.0003 0.0001 0.0025 0.0004 0.0038 0.0006 0.0011 0 0.0001 0 0.0001 0
Mitochondria 0.005 0.0003 0.0019 0.0058 0.001 0.0013 0.0016 0.001 0.0044 0.0028 0.0078 0.0016 0.0129 0.0005 0.0086 0.0001 0.0102 0.0004
None 0.0057 0 0.0013 0.0133 0.0009 0.0034 0.0007 0 0.001 0.0006 0.0029 0.0006 0.0016 0 0.0003 0 0.0007 0
Nuclear Periphery 0.0404 0.0122 0.0274 0.0391 0.0236 0.0275 0.0387 0.0195 0.0378 0.0316 0.0595 0.0138 0.0661 0.0152 0.0119 0.0271 0.0759 0.0239
Nucleolus 0.0454 0.0095 0.0137 0.0587 0.1098 0.0282 0.0248 0.0067 0.0099 0.0146 0.065 0.0259 0.0522 0.0064 0.0052 0.0005 0.0302 0.0238
Nucleus 0.796 0.9769 0.9125 0.7914 0.8384 0.9212 0.9058 0.9618 0.8937 0.9163 0.7683 0.9495 0.7889 0.9766 0.9573 0.9719 0.8208 0.948
Peroxisomes 0.0026 0 0.001 0.0024 0 0.0001 0.0001 0 0.0023 0.0013 0.0002 0.0001 0.0002 0 0.0001 0 0.0001 0
Punctate Nuclear 0.0282 0.0001 0.003 0.0155 0.002 0.0031 0.0154 0.0001 0.01 0.0065 0.0048 0.0004 0.0218 0.0002 0.0063 0 0.0371 0.0011
Vacuole 0.0048 0.0002 0.0015 0.0028 0.005 0.0017 0.002 0.0005 0.003 0.0011 0.0127 0.0014 0.0058 0.0001 0.0002 0 0.0014 0.0003
Vacuole Periphery 0.0037 0.0003 0.0022 0.0054 0.0022 0.0019 0.002 0.0007 0.0068 0.0012 0.0128 0.0025 0.0066 0.0002 0.0005 0.0001 0.004 0.0005

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 93.6222 88.475 95.8168 120.5194 78.0334 90.8969 118.47 104.4366 117.761 100.0462
Translational Efficiency 1.8203 1.832 1.7818 1.4122 1.7007 2.1723 1.4743 1.7978 1.5683 1.5261

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
23 1593 2524 1948 1953 1899 2214 1580 1976 3492 4738 3528

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1990.57 2142.70 2507.76 2121.61 2113.23 2343.28 2493.35 2601.62 2111.80 2251.78 2501.03 2336.58
Standard Deviation 870.00 585.30 509.19 632.45 443.05 498.20 564.65 570.69 450.55 548.85 535.87 650.92
Intensity Change Log 2 — 0.106248 0.333218 0.091978 — 0.149080 0.238636 0.299960 — 0.128463 0.285288 0.202813

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.002639 0.001173 0.001037 0.000988 0.001168 0.001036 0.000843 0.001724 0.001185 0.001098 0.000946 0.001318
Bud Neck 0.099829 0.010631 0.020595 0.019468 0.010948 0.008415 0.023716 0.020301 0.011982 0.009426 0.022054 0.019841
Bud Site 0.008160 0.000695 0.000728 0.005453 0.000357 0.000409 0.001672 0.001038 0.000447 0.000539 0.001169 0.003476
Cell Periphery 0.001937 0.000154 0.000056 0.000299 0.000085 0.000087 0.000170 0.000111 0.000106 0.000117 0.000110 0.000215
Cytoplasm 0.032436 0.005196 0.000573 0.011073 0.000640 0.001051 0.002469 0.002417 0.001010 0.002942 0.001459 0.007196
Cytoplasmic Foci 0.005512 0.000264 0.000083 0.002755 0.000054 0.000108 0.000467 0.000328 0.000118 0.000179 0.000262 0.001668
Eisosomes 0.000043 0.000028 0.000020 0.000022 0.000026 0.000034 0.000025 0.000022 0.000026 0.000031 0.000022 0.000022
Endoplasmic Reticulum 0.010492 0.001132 0.001628 0.001138 0.002223 0.001392 0.000969 0.001158 0.002319 0.001273 0.001320 0.001147
Endosome 0.003204 0.000153 0.000065 0.004834 0.000100 0.000096 0.000436 0.000188 0.000136 0.000122 0.000238 0.002753
Golgi 0.000983 0.000122 0.000084 0.001281 0.000076 0.000088 0.000184 0.000335 0.000087 0.000104 0.000131 0.000857
Lipid Particles 0.001477 0.000036 0.000011 0.000317 0.000012 0.000082 0.000073 0.000027 0.000029 0.000061 0.000040 0.000187
Mitochondria 0.011685 0.002764 0.003612 0.003796 0.002847 0.002290 0.003069 0.006538 0.002950 0.002506 0.003359 0.005024
Mitotic Spindle 0.003573 0.000213 0.000541 0.021286 0.000080 0.000458 0.003297 0.002463 0.000120 0.000346 0.001829 0.012857
None 0.019996 0.007629 0.000700 0.009426 0.001120 0.002462 0.004302 0.001350 0.001339 0.004819 0.002383 0.005809
Nuclear Periphery 0.069655 0.002319 0.007961 0.003531 0.006999 0.002719 0.001854 0.013810 0.007728 0.002536 0.005107 0.008135
Nuclear Periphery Foci 0.059925 0.000827 0.000437 0.003427 0.000761 0.000826 0.000301 0.000996 0.001450 0.000827 0.000373 0.002338
Nucleolus 0.060229 0.093943 0.052227 0.024640 0.116594 0.127651 0.039227 0.103094 0.115938 0.112274 0.046153 0.059776
Nucleus 0.574872 0.867408 0.908040 0.880172 0.852431 0.847699 0.915596 0.839608 0.849200 0.856690 0.911571 0.862005
Peroxisomes 0.002101 0.000553 0.000527 0.001250 0.000463 0.000813 0.000487 0.001008 0.000483 0.000694 0.000508 0.001142
Vacuole 0.027145 0.001644 0.000322 0.003385 0.000835 0.000597 0.000471 0.001242 0.001141 0.001074 0.000392 0.002425
Vacuole Periphery 0.004108 0.003116 0.000754 0.001458 0.002183 0.001688 0.000370 0.002241 0.002205 0.002339 0.000574 0.001809

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 3.40 3.63 3.71 2.15 0.83 2.70 5.83 -9.06 -11.11 -13.54 1.68 4.48 -3.69 -5.57 -8.81
Bud Neck 3.48 3.06 3.02 -10.63 -0.81 3.91 -16.23 -11.15 -14.05 3.35 3.41 -15.46 -11.88 -17.28 1.65
Bud Site 2.82 2.82 1.63 -5.42 -5.38 -1.35 -7.37 -2.84 -2.55 1.63 -1.26 -5.19 -6.70 -6.14 -4.19
Cell Periphery 3.48 3.66 3.32 -2.96 -7.26 -0.18 -3.51 -1.59 -1.16 1.66 -0.74 -0.25 -4.01 -3.37 -3.97
Cytoplasm 2.78 3.32 2.23 -3.52 -9.15 -1.66 -8.46 -2.96 -2.29 -0.64 -4.29 -3.22 -8.57 -4.87 -7.79
Cytoplasmic Foci 1.84 1.91 1.39 -3.64 -4.28 -2.08 -2.17 -2.52 -2.20 -0.76 -1.55 -1.46 -4.72 -4.34 -3.93
Eisosomes 1.72 2.47 2.33 3.93 -1.15 -2.73 -1.14 1.64 3.64 3.11 -2.74 1.44 2.36 4.94 1.48
Endoplasmic Reticulum 2.02 1.93 2.02 -0.09 5.59 4.78 7.65 6.29 2.57 -1.85 6.05 6.02 6.92 2.06 3.01
Endosome 2.95 3.05 0.51 -3.86 -4.03 -0.37 -1.65 -1.90 -1.69 0.90 0.02 -0.84 -3.99 -3.98 -3.76
Golgi 4.80 5.05 0.52 -3.40 -3.60 -1.46 -2.28 -2.32 -2.18 -1.33 -2.18 -2.37 -4.29 -4.05 -3.77
Lipid Particles 2.22 2.27 1.87 -3.75 -4.44 -1.53 -2.45 -2.81 0.66 0.54 -1.34 -0.83 -4.31 -2.29 -3.83
Mitochondria 2.82 2.47 2.49 -5.26 0.24 4.32 -4.28 -14.87 -17.00 -11.88 3.61 -5.84 -12.28 -15.99 -8.29
Mitotic Spindle 1.37 1.02 -4.92 -8.79 -8.37 -1.97 -4.94 -3.39 -2.77 0.94 -1.94 -5.73 -9.42 -9.10 -6.81
None 0.74 1.29 0.74 -0.03 -7.80 -2.20 -4.22 -1.84 1.60 3.65 -4.98 -2.75 -6.86 -0.09 -4.46
Nuclear Periphery 2.23 2.03 2.20 -2.39 5.51 4.70 6.10 -3.94 -6.92 -7.59 5.89 2.34 -0.18 -7.11 -2.90
Nuclear Periphery Foci 2.68 2.70 2.59 -2.93 -3.86 -0.63 3.27 -2.16 -0.88 -4.58 1.43 3.28 -1.34 -3.00 -4.99
Nucleolus -2.04 -0.03 1.27 13.64 7.86 -1.84 16.65 2.05 3.76 -13.51 0.67 15.57 11.11 12.55 -5.69
Nucleus -5.24 -5.81 -5.41 -1.39 3.98 0.82 -9.75 3.25 2.41 12.10 -1.17 -10.62 -1.03 0.13 10.95
Peroxisomes 0.96 0.95 0.67 -1.30 -1.24 -5.99 -1.19 -7.94 -2.59 -6.69 -4.85 -1.55 -3.13 -1.73 -2.70
Vacuole 2.21 2.35 2.09 -2.03 -5.59 2.50 1.12 -2.71 -3.34 -3.03 -0.32 3.68 -4.08 -3.77 -6.33
Vacuole Periphery 0.88 3.30 3.05 3.74 -0.96 1.45 9.39 0.09 -1.12 -5.85 -0.40 8.46 2.76 2.49 -5.10
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Components••••••••••••••••actinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
Standard name
Human Ortholog
Description Subunit of the 19S regulatory particle of the 26S proteasome lid; synthetically lethal with RPT1, which is an ATPase component of the 19S regulatory particle; physically interacts with Nob1p and Rpn3p; protein abundance increases in response to DNA replication stress
Localization
Cell Percentages nucleus (91%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-5

Rpn12

Rpn12


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rpn12-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available