Standard name
Human Ortholog
Description Subunit of the 19S regulatory particle of the 26S proteasome lid; synthetically lethal with RPT1, which is an ATPase component of the 19S regulatory particle; physically interacts with Nob1p and Rpn3p; protein abundance increases in response to DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 – 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 – 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 – 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 – 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 – 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0.05 – 0.09 0 0.09 – 0.06 0.15 0.07 0.11 0.08 0.15 0.2 0.07 0.09 0.11 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 – 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 – 0 0 0 – 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 – 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 – 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.81 0.76 0.7 – 0.61 0.47 0.33 – 0.4 0.48 0.43 0.42 0.69 0.55 0.53 0.62 0.61 0.5 0.72 0.76 0.74 0.77 0.58 0.47
Nuclear Periphery 0 0 0 – 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.11 0.1 0 – 0.09 0 0.1 – 0.12 0.11 0.17 0.21 0 0 0 0 0.05 0.06 0.13 0.1 0.19 0.09 0.15 0.14
Peroxisomes 0 0 0 – 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 – 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.05 0.19 0.25 – 0.22 0.47 0.52 – 0.48 0.22 0.31 0.42 0.19 0.26 0.26 0.26 0.23 0.35 0.09 0.07 0.05 0.11 0.19 0.31
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 – 0 0 0 – 0 0 0 0 1 1 1 1 2 1 0 0 0 0 1 0
Bud 0 0 0 – 0 0 0 – 0 0 0 0 0 0 0 0 1 1 1 2 0 4 8 8
Bud Neck 0 0 0 – 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 – 0 0 0 – 1 0 3 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 – 0 0 0 – 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0
Cytoplasm 2 4 1 – 2 2 7 – 8 4 6 2 18 54 78 29 30 30 0 3 0 1 3 3
Endoplasmic Reticulum 0 0 0 – 0 0 0 – 0 0 0 0 0 0 0 10 8 9 0 0 0 0 0 1
Endosome 0 0 0 – 0 0 0 – 0 0 0 1 2 2 1 6 1 1 0 2 0 0 5 3
Golgi 0 0 0 – 0 2 3 – 0 0 0 0 3 5 2 3 10 4 0 0 0 0 1 1
Mitochondria 0 1 0 – 1 0 0 – 1 1 3 0 0 0 2 5 9 4 1 0 0 0 0 4
Nucleus 51 102 14 – 14 22 26 – 54 13 39 8 148 191 205 262 214 135 83 122 41 264 237 224
Nuclear Periphery 0 0 0 – 0 0 0 – 0 0 1 0 1 0 4 6 4 1 0 0 1 0 2 5
Nucleolus 7 13 0 – 2 2 8 – 16 3 15 4 8 15 12 18 17 15 15 16 10 30 60 68
Peroxisomes 0 0 0 – 0 0 0 – 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 – 0 0 0 – 0 0 0 0 1 0 1 0 0 0 0 0 0 1 2 6
Vac/Vac Membrane 3 25 5 – 5 22 41 – 65 6 28 8 40 89 100 108 80 95 10 11 2 37 79 149
Unique Cell Count 63 135 20 23 47 79 135 27 90 19 216 349 389 421 348 269 116 161 56 343 408 480
Labelled Cell Count 63 145 20 24 50 85 145 27 95 23 222 357 408 448 376 296 116 161 56 343 408 480


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 31.3 25.6 36.4 – 19.6 32.8 31.1 – 26.2 23.2 23.0 20.1 31.8 30.3 30.9 25.6 33.5 24.6 33.7 36.2 38.5
Std Deviation (1e-4) 8.0 7.4 8.1 – 7.3 6.7 7.9 – 7.7 8.9 8.8 5.5 10.9 10.3 10.8 9.0 12.3 11.5 9.9 10.2 11.8
Intensity Change (Log2) – – – – -0.89 -0.15 -0.23 – -0.47 -0.65 -0.66 -0.86 -0.19 -0.26 -0.24 -0.51 -0.12 -0.56 -0.11 -0.01 0.08


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches – 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud – 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck – 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site – 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery – 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cytoplasm – 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum – 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Endosome – 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi – 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria – 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleus – -0.6 -1.7 -3.0 – -2.5 -1.5 -2.2 -1.8 -0.1 -1.3 -1.5 -0.7 -0.8 -1.7
Nuclear Periphery – 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus – 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Peroxisomes – 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole – 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole – 0 1.7 2.2 – 1.9 0 0.5 1.1 0 0.1 0.1 0.1 0 0.9

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 25.2517 31.5093 27.8843 24.4376 28.2358 27.6625 39.0762 43.2994 37.9967 36.9619 37.6701 39.7031 28.0217 35.1046 32.319 33.1472 29.3735 34.343
Actin 0.0068 0 0.0115 0.0105 0.0003 0.0014 0.0006 0.0008 0.0062 0.007 0.0059 0.0001 0.0029 0.0001 0.0051 0 0.005 0.0004
Bud 0.0005 0 0.0001 0.0003 0.0001 0.0001 0.0003 0.0001 0.0002 0.0007 0.0003 0.0001 0.0004 0 0.0002 0 0.0004 0
Bud Neck 0.0009 0.0001 0.0002 0.0002 0.0009 0.0008 0.0001 0.0001 0.0002 0.0004 0.0007 0.0006 0.0003 0.0001 0.0001 0 0.0005 0.0006
Bud Periphery 0.0008 0 0.0002 0.0003 0.0002 0.0003 0.0002 0 0.0002 0.0005 0.0005 0.0001 0.0004 0 0.0002 0 0.0004 0.0001
Bud Site 0.0015 0 0.0003 0.0007 0.0003 0.0001 0.0003 0 0.0005 0.0006 0.0004 0 0.0007 0 0.0004 0 0.0001 0
Cell Periphery 0.0008 0 0.0003 0.0005 0.0011 0.0002 0.0003 0 0.0004 0.0001 0.0007 0.0002 0.0002 0 0 0 0.0001 0.0001
Cytoplasm 0.0099 0.0003 0.0067 0.0063 0.0107 0.0024 0.0045 0.0017 0.0063 0.0072 0.0238 0.0008 0.0171 0.0006 0.0022 0.0001 0.0052 0.0002
Cytoplasmic Foci 0.0139 0 0.0061 0.0212 0.001 0.0004 0.001 0.0003 0.0063 0.0032 0.0166 0.0014 0.0167 0 0.0004 0 0.0004 0
Eisosomes 0.0001 0 0.0001 0.0003 0 0 0 0 0.0001 0 0.0001 0 0 0 0 0 0 0
Endoplasmic Reticulum 0.0044 0 0.0017 0.0021 0.0009 0.0009 0.0004 0.0002 0.0021 0.0009 0.0031 0.0001 0.002 0 0.0002 0 0.0022 0.0001
Endosome 0.0159 0 0.0038 0.0114 0.0005 0.0042 0.0007 0.0025 0.0049 0.0022 0.0088 0.0003 0.0018 0 0.0006 0 0.0047 0.0002
Golgi 0.0026 0 0.0012 0.0019 0 0.0003 0.0001 0.0038 0.0013 0.0006 0.0011 0 0.0002 0 0.0003 0 0.0003 0
Lipid Particles 0.0101 0 0.0033 0.01 0.0009 0.0005 0.0003 0.0001 0.0025 0.0004 0.0038 0.0006 0.0011 0 0.0001 0 0.0001 0
Mitochondria 0.005 0.0003 0.0019 0.0058 0.001 0.0013 0.0016 0.001 0.0044 0.0028 0.0078 0.0016 0.0129 0.0005 0.0086 0.0001 0.0102 0.0004
None 0.0057 0 0.0013 0.0133 0.0009 0.0034 0.0007 0 0.001 0.0006 0.0029 0.0006 0.0016 0 0.0003 0 0.0007 0
Nuclear Periphery 0.0404 0.0122 0.0274 0.0391 0.0236 0.0275 0.0387 0.0195 0.0378 0.0316 0.0595 0.0138 0.0661 0.0152 0.0119 0.0271 0.0759 0.0239
Nucleolus 0.0454 0.0095 0.0137 0.0587 0.1098 0.0282 0.0248 0.0067 0.0099 0.0146 0.065 0.0259 0.0522 0.0064 0.0052 0.0005 0.0302 0.0238
Nucleus 0.796 0.9769 0.9125 0.7914 0.8384 0.9212 0.9058 0.9618 0.8937 0.9163 0.7683 0.9495 0.7889 0.9766 0.9573 0.9719 0.8208 0.948
Peroxisomes 0.0026 0 0.001 0.0024 0 0.0001 0.0001 0 0.0023 0.0013 0.0002 0.0001 0.0002 0 0.0001 0 0.0001 0
Punctate Nuclear 0.0282 0.0001 0.003 0.0155 0.002 0.0031 0.0154 0.0001 0.01 0.0065 0.0048 0.0004 0.0218 0.0002 0.0063 0 0.0371 0.0011
Vacuole 0.0048 0.0002 0.0015 0.0028 0.005 0.0017 0.002 0.0005 0.003 0.0011 0.0127 0.0014 0.0058 0.0001 0.0002 0 0.0014 0.0003
Vacuole Periphery 0.0037 0.0003 0.0022 0.0054 0.0022 0.0019 0.002 0.0007 0.0068 0.0012 0.0128 0.0025 0.0066 0.0002 0.0005 0.0001 0.004 0.0005

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 93.6222 88.475 95.8168 120.5194 78.0334 90.8969 118.47 104.4366 117.761 100.0462
Translational Efficiency 1.8203 1.832 1.7818 1.4122 1.7007 2.1723 1.4743 1.7978 1.5683 1.5261

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
23 1593 2524 1948 1953 1899 2214 1580 1976 3492 4738 3528

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1990.57 2142.70 2507.76 2121.61 2113.23 2343.28 2493.35 2601.62 2111.80 2251.78 2501.03 2336.58
Standard Deviation 870.00 585.30 509.19 632.45 443.05 498.20 564.65 570.69 450.55 548.85 535.87 650.92
Intensity Change Log 2 — 0.106248 0.333218 0.091978 — 0.149080 0.238636 0.299960 — 0.128463 0.285288 0.202813

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.002639 0.001173 0.001037 0.000988 0.001168 0.001036 0.000843 0.001724 0.001185 0.001098 0.000946 0.001318
Bud Neck 0.099829 0.010631 0.020595 0.019468 0.010948 0.008415 0.023716 0.020301 0.011982 0.009426 0.022054 0.019841
Bud Site 0.008160 0.000695 0.000728 0.005453 0.000357 0.000409 0.001672 0.001038 0.000447 0.000539 0.001169 0.003476
Cell Periphery 0.001937 0.000154 0.000056 0.000299 0.000085 0.000087 0.000170 0.000111 0.000106 0.000117 0.000110 0.000215
Cytoplasm 0.032436 0.005196 0.000573 0.011073 0.000640 0.001051 0.002469 0.002417 0.001010 0.002942 0.001459 0.007196
Cytoplasmic Foci 0.005512 0.000264 0.000083 0.002755 0.000054 0.000108 0.000467 0.000328 0.000118 0.000179 0.000262 0.001668
Eisosomes 0.000043 0.000028 0.000020 0.000022 0.000026 0.000034 0.000025 0.000022 0.000026 0.000031 0.000022 0.000022
Endoplasmic Reticulum 0.010492 0.001132 0.001628 0.001138 0.002223 0.001392 0.000969 0.001158 0.002319 0.001273 0.001320 0.001147
Endosome 0.003204 0.000153 0.000065 0.004834 0.000100 0.000096 0.000436 0.000188 0.000136 0.000122 0.000238 0.002753
Golgi 0.000983 0.000122 0.000084 0.001281 0.000076 0.000088 0.000184 0.000335 0.000087 0.000104 0.000131 0.000857
Lipid Particles 0.001477 0.000036 0.000011 0.000317 0.000012 0.000082 0.000073 0.000027 0.000029 0.000061 0.000040 0.000187
Mitochondria 0.011685 0.002764 0.003612 0.003796 0.002847 0.002290 0.003069 0.006538 0.002950 0.002506 0.003359 0.005024
Mitotic Spindle 0.003573 0.000213 0.000541 0.021286 0.000080 0.000458 0.003297 0.002463 0.000120 0.000346 0.001829 0.012857
None 0.019996 0.007629 0.000700 0.009426 0.001120 0.002462 0.004302 0.001350 0.001339 0.004819 0.002383 0.005809
Nuclear Periphery 0.069655 0.002319 0.007961 0.003531 0.006999 0.002719 0.001854 0.013810 0.007728 0.002536 0.005107 0.008135
Nuclear Periphery Foci 0.059925 0.000827 0.000437 0.003427 0.000761 0.000826 0.000301 0.000996 0.001450 0.000827 0.000373 0.002338
Nucleolus 0.060229 0.093943 0.052227 0.024640 0.116594 0.127651 0.039227 0.103094 0.115938 0.112274 0.046153 0.059776
Nucleus 0.574872* 0.867408* 0.908040* 0.880172* 0.852431* 0.847699* 0.915596* 0.839608* 0.849200* 0.856690* 0.911571* 0.862005*
Peroxisomes 0.002101 0.000553 0.000527 0.001250 0.000463 0.000813 0.000487 0.001008 0.000483 0.000694 0.000508 0.001142
Vacuole 0.027145 0.001644 0.000322 0.003385 0.000835 0.000597 0.000471 0.001242 0.001141 0.001074 0.000392 0.002425
Vacuole Periphery 0.004108 0.003116 0.000754 0.001458 0.002183 0.001688 0.000370 0.002241 0.002205 0.002339 0.000574 0.001809

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 3.40 3.63 3.71 2.15 0.83 2.70 5.83 -9.06 -11.11 -13.54 1.68 4.48 -3.69 -5.57 -8.81
Bud Neck 3.48 3.06 3.02 -10.63 -0.81 3.91 -16.23 -11.15 -14.05 3.35 3.41 -15.46 -11.88 -17.28 1.65
Bud Site 2.82 2.82 1.63 -5.42 -5.38 -1.35 -7.37 -2.84 -2.55 1.63 -1.26 -5.19 -6.70 -6.14 -4.19
Cell Periphery 3.48 3.66 3.32 -2.96 -7.26 -0.18 -3.51 -1.59 -1.16 1.66 -0.74 -0.25 -4.01 -3.37 -3.97
Cytoplasm 2.78 3.32 2.23 -3.52 -9.15 -1.66 -8.46 -2.96 -2.29 -0.64 -4.29 -3.22 -8.57 -4.87 -7.79
Cytoplasmic Foci 1.84 1.91 1.39 -3.64 -4.28 -2.08 -2.17 -2.52 -2.20 -0.76 -1.55 -1.46 -4.72 -4.34 -3.93
Eisosomes 1.72 2.47 2.33 3.93 -1.15 -2.73 -1.14 1.64 3.64 3.11 -2.74 1.44 2.36 4.94 1.48
Endoplasmic Reticulum 2.02 1.93 2.02 -0.09 5.59 4.78 7.65 6.29 2.57 -1.85 6.05 6.02 6.92 2.06 3.01
Endosome 2.95 3.05 0.51 -3.86 -4.03 -0.37 -1.65 -1.90 -1.69 0.90 0.02 -0.84 -3.99 -3.98 -3.76
Golgi 4.80 5.05 0.52 -3.40 -3.60 -1.46 -2.28 -2.32 -2.18 -1.33 -2.18 -2.37 -4.29 -4.05 -3.77
Lipid Particles 2.22 2.27 1.87 -3.75 -4.44 -1.53 -2.45 -2.81 0.66 0.54 -1.34 -0.83 -4.31 -2.29 -3.83
Mitochondria 2.82 2.47 2.49 -5.26 0.24 4.32 -4.28 -14.87 -17.00 -11.88 3.61 -5.84 -12.28 -15.99 -8.29
Mitotic Spindle 1.37 1.02 -4.92 -8.79 -8.37 -1.97 -4.94 -3.39 -2.77 0.94 -1.94 -5.73 -9.42 -9.10 -6.81
None 0.74 1.29 0.74 -0.03 -7.80 -2.20 -4.22 -1.84 1.60 3.65 -4.98 -2.75 -6.86 -0.09 -4.46
Nuclear Periphery 2.23 2.03 2.20 -2.39 5.51 4.70 6.10 -3.94 -6.92 -7.59 5.89 2.34 -0.18 -7.11 -2.90
Nuclear Periphery Foci 2.68 2.70 2.59 -2.93 -3.86 -0.63 3.27 -2.16 -0.88 -4.58 1.43 3.28 -1.34 -3.00 -4.99
Nucleolus -2.04 -0.03 1.27 13.64 7.86 -1.84 16.65 2.05 3.76 -13.51 0.67 15.57 11.11 12.55 -5.69
Nucleus -5.24 -5.81 -5.41 -1.39 3.98 0.82 -9.75 3.25 2.41 12.10 -1.17 -10.62 -1.03 0.13 10.95
Peroxisomes 0.96 0.95 0.67 -1.30 -1.24 -5.99 -1.19 -7.94 -2.59 -6.69 -4.85 -1.55 -3.13 -1.73 -2.70
Vacuole 2.21 2.35 2.09 -2.03 -5.59 2.50 1.12 -2.71 -3.34 -3.03 -0.32 3.68 -4.08 -3.77 -6.33
Vacuole Periphery 0.88 3.30 3.05 3.74 -0.96 1.45 9.39 0.09 -1.12 -5.85 -0.40 8.46 2.76 2.49 -5.10
Standard name
Human Ortholog
Description Subunit of the 19S regulatory particle of the 26S proteasome lid; synthetically lethal with RPT1, which is an ATPase component of the 19S regulatory particle; physically interacts with Nob1p and Rpn3p; protein abundance increases in response to DNA replication stress
Localization
Cell Percentages nucleus (91%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-5

Rpn12

Rpn12


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rpn12-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available