Standard name
Human Ortholog
Description Evolutionarily conserved 19S regulatory particle assembly-chaperone; proteasome-interacting protein involved in the assembly of the base subcomplex of the 19S proteasome regulatory particle (RP); null mutants accumulate ubiquitinated Gcn4p and display decreased 26S proteasome stability; interacts with Rpt5p
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06 0 0 0 0 0 0
Cytoplasm 0.95 0.97 1.0 0.96 0.97 0.92 0.79 0.75 0.61 0.67 0.61 0.6 0.99 0.99 0.99 0.97 0.96 0.96 0.93 0.94 0.98 0.95 0.93 0.94
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0.05 0.05 0.2 0.27 0.24 0.44 0.42 0.51 0.44 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.05 0 0 0 0 0 0.06 0.11 0.16 0.1 0.13 0.08 0 0 0 0 0.05 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 1
Bud 0 2 0 0 2 1 3 3 3 3 4 4 0 0 0 0 0 0 1 0 0 0 4 3
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 3 1 2 2 4 4 2 1 3 2 0 3 9 5 6 9 21 22 0 0 0 0 0 0
Cytoplasm 236 147 180 129 176 255 276 282 140 252 123 131 454 639 688 305 420 379 252 147 181 346 595 552
Endoplasmic Reticulum 0 0 1 0 0 1 1 2 0 2 0 1 0 4 1 16 10 6 1 0 1 0 1 3
Endosome 1 0 0 0 0 0 0 1 0 0 0 0 1 1 0 0 2 0 1 0 0 0 7 3
Golgi 0 1 0 1 0 0 0 0 0 0 0 0 0 0 1 0 0 4 2 0 0 0 1 1
Mitochondria 10 2 1 7 9 56 93 89 101 159 103 97 0 0 0 3 1 4 3 1 0 0 7 5
Nucleus 0 2 0 1 0 1 9 5 3 8 5 10 0 3 3 3 5 5 0 0 0 4 1 3
Nuclear Periphery 1 0 0 1 0 2 1 1 2 1 1 5 0 0 0 0 0 0 0 0 0 0 1 1
Nucleolus 0 0 0 1 0 1 2 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 1 1
Vac/Vac Membrane 12 2 0 2 2 3 20 41 36 36 27 17 2 0 6 13 22 14 3 2 0 5 5 7
Unique Cell Count 249 151 180 135 182 278 348 374 229 376 201 220 457 644 692 314 437 396 270 158 184 363 638 591
Labelled Cell Count 263 157 184 144 193 324 407 425 288 463 263 269 466 652 705 349 481 435 270 158 184 363 638 591


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.7 6.5 6.5 6.2 6.3 5.3 5.2 5.4 5.4 5.1 4.9 5.2 7.9 8.1 8.2 9.2 9.0 9.4 7.5 7.8 8.0
Std Deviation (1e-4) 0.8 1.1 1.3 1.8 1.9 1.1 1.1 1.3 1.5 1.0 1.5 1.3 1.1 1.2 1.2 1.7 1.7 1.7 1.3 1.4 1.3
Intensity Change (Log2) – – – -0.07 -0.06 -0.29 -0.32 -0.27 -0.28 -0.35 -0.42 -0.32 0.27 0.3 0.32 0.49 0.47 0.52 0.2 0.26 0.3


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 2.2 2.5
Cytoplasm -2.9 -2.5 -4.0 -6.6 -7.3 -9.5 -8.7 -9.4 -9.7 -1.1 -1.2 -1.0 -2.3 -2.7 -2.8
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 2.7 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 6.2 7.5 6.9 10.1 10.2 0 10.1 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 3.3 4.6 5.6 4.3 5.1 3.8 0 0 0 0 3.1 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.5554 3.4903 3.1044 2.5478 2.3347 2.9124 1.2311 2.2861 1.882 1.2655 1.1453 1.7179 1.7544 2.5714 2.1807 1.8384 1.5425 1.7215
Actin 0.0253 0.0004 0.0202 0.0039 0.0282 0.0134 0.0573 0.0089 0.0108 0.0381 0.0024 0.0072 0.0188 0.0016 0.0064 0.0024 0.0137 0.0054
Bud 0.0004 0.0003 0.0022 0.0004 0.0003 0.0005 0.0013 0.0023 0.0002 0.0026 0.0002 0.003 0.0125 0.0113 0.0138 0.0043 0.0013 0.0032
Bud Neck 0.0013 0.0003 0.0044 0.0006 0.0006 0.0018 0.0033 0.0003 0.0003 0.0034 0.0012 0.0014 0.0009 0.0003 0.0007 0.0016 0.0022 0.0028
Bud Periphery 0.0004 0.0001 0.0022 0.0005 0.0002 0.0004 0.0018 0.002 0.0001 0.0056 0.0001 0.0017 0.0074 0.0032 0.0047 0.0019 0.0011 0.0013
Bud Site 0.0026 0.0021 0.0127 0.0005 0.0002 0.0003 0.005 0.0054 0.0005 0.0198 0.0003 0.0003 0.0147 0.0108 0.0082 0.001 0.0011 0.0005
Cell Periphery 0.0002 0.0001 0.0003 0.0001 0 0.0001 0.0003 0.0001 0 0.0005 0 0.0001 0.0007 0.0003 0.0003 0.0001 0.0003 0.0001
Cytoplasm 0.7483 0.8351 0.7958 0.7696 0.8589 0.8377 0.6731 0.8399 0.8544 0.8034 0.8604 0.8452 0.6977 0.8403 0.801 0.7611 0.7634 0.8446
Cytoplasmic Foci 0.0145 0.0017 0.0118 0.0124 0.0093 0.0127 0.0288 0.0057 0.0065 0.0142 0.0143 0.0091 0.0264 0.0168 0.0141 0.0487 0.0186 0.0178
Eisosomes 0.0003 0 0.0001 0.0001 0.0001 0.0001 0.0008 0 0 0.0002 0 0.0001 0.0004 0 0 0 0.0004 0
Endoplasmic Reticulum 0.0088 0.0017 0.0031 0.0102 0.0029 0.0044 0.0074 0.0032 0.0032 0.0035 0.006 0.0033 0.0085 0.002 0.0027 0.0026 0.0035 0.0025
Endosome 0.0206 0.0006 0.0084 0.0205 0.0053 0.0089 0.0386 0.004 0.008 0.0151 0.0104 0.01 0.0264 0.0016 0.0139 0.0354 0.0146 0.014
Golgi 0.0057 0.0001 0.0048 0.0023 0.0012 0.0037 0.0136 0.0024 0.0024 0.0069 0.0018 0.0048 0.003 0.0002 0.0043 0.003 0.0051 0.0027
Lipid Particles 0.0087 0 0.0024 0.0047 0.0023 0.0045 0.0242 0.0012 0.0092 0.0031 0.0081 0.0025 0.0049 0.0002 0.0041 0.0092 0.0197 0.0009
Mitochondria 0.0057 0.0001 0.0017 0.0065 0.0004 0.0012 0.0056 0.0025 0.0025 0.0165 0.0004 0.0032 0.017 0.0025 0.0044 0.0012 0.0183 0.0028
None 0.1043 0.0464 0.0452 0.0863 0.0441 0.036 0.0699 0.027 0.0311 0.0317 0.0215 0.0533 0.0976 0.077 0.0746 0.068 0.0943 0.0547
Nuclear Periphery 0.0091 0.0039 0.0033 0.0166 0.003 0.0039 0.0098 0.0042 0.0023 0.0033 0.0033 0.0034 0.0086 0.0025 0.0041 0.0026 0.0041 0.0043
Nucleolus 0.0006 0.0001 0.0006 0.0015 0.0001 0.0002 0.0015 0.0001 0.0001 0.0004 0.0003 0.0002 0.0048 0.0011 0.001 0.0006 0.0059 0.0003
Nucleus 0.0286 0.1052 0.0636 0.0525 0.0279 0.0607 0.0232 0.0867 0.0592 0.0177 0.0469 0.0463 0.0176 0.0209 0.0255 0.0098 0.0152 0.0301
Peroxisomes 0.0061 0 0.0066 0.0016 0.0112 0.0043 0.0144 0.001 0.0057 0.0031 0.0105 0.0006 0.0017 0.0004 0.0044 0.0177 0.0049 0.0018
Punctate Nuclear 0.0056 0.0009 0.0038 0.0059 0.0021 0.0036 0.0131 0.0017 0.001 0.004 0.0091 0.0023 0.0145 0.0057 0.0093 0.0232 0.008 0.0084
Vacuole 0.0021 0.0007 0.0061 0.0021 0.0016 0.0013 0.0056 0.0011 0.002 0.0051 0.0026 0.0016 0.012 0.001 0.0021 0.0041 0.0031 0.0012
Vacuole Periphery 0.0009 0.0001 0.0007 0.0013 0.0003 0.0003 0.0016 0.0003 0.0003 0.0017 0.0004 0.0004 0.0039 0.0002 0.0005 0.0014 0.0013 0.0005

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 5.7194 1.5732 7.0042 10.0396 9.6971 6.1559 10.9356 11.5408 12.0485 10.5256
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency –

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
937 142 416 1260 1514 1438 490 181 2451 1580 906 1441

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 737.64 942.30 1022.57 930.23 799.52 814.01 990.59 1010.33 775.86 825.54 1005.27 940.29
Standard Deviation 94.39 122.65 114.21 130.74 103.88 116.13 130.81 144.56 104.76 122.36 124.49 135.19
Intensity Change Log 2 — 0.353270 0.471211 0.334671 — 0.025912 0.309154 0.337621 — 0.192280 0.389194 0.336206

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000127 0.000410 0.000570 0.001271 0.000127 0.000309 0.000294 0.000215 0.000127 0.000318 0.000421 0.001138
Bud Neck 0.004606 0.004227 0.003149 0.002437 0.002400 0.006293 0.003453 0.023354 0.003243 0.006107 0.003313 0.005064
Bud Site 0.004961 0.013264 0.005362 0.016318 0.003145 0.011037 0.005594 0.054294 0.003839 0.011237 0.005487 0.021088
Cell Periphery 0.000172 0.000116 0.000199 0.000122 0.000164 0.000434 0.000192 0.000059 0.000167 0.000406 0.000195 0.000114
Cytoplasm 0.585251* 0.554908* 0.410955* 0.594386* 0.686558* 0.658564* 0.515876* 0.462658* 0.647829* 0.649248* 0.467700* 0.577840*
Cytoplasmic Foci 0.205731* 0.190696* 0.014944 0.032072 0.114663 0.122595 0.029798 0.031454 0.149477 0.128716 0.022977 0.031994
Eisosomes 0.000087 0.000055 0.000054 0.000041 0.000048 0.000053 0.000073 0.000031 0.000063 0.000054 0.000064 0.000040
Endoplasmic Reticulum 0.002186 0.001599 0.006481 0.001718 0.001446 0.000726 0.002441 0.000702 0.001729 0.000804 0.004296 0.001590
Endosome 0.008816 0.013950 0.005069 0.006511 0.006333 0.011533 0.003575 0.014301 0.007282 0.011750 0.004261 0.007490
Golgi 0.002079 0.003711 0.000139 0.003618 0.000710 0.002786 0.000089 0.000928 0.001233 0.002870 0.000112 0.003280
Lipid Particles 0.004741 0.001686 0.002147 0.001102 0.002487 0.002521 0.001749 0.000818 0.003349 0.002446 0.001932 0.001066
Mitochondria 0.001089 0.000257 0.000565 0.000801 0.000493 0.003042 0.000186 0.000819 0.000721 0.002792 0.000360 0.000803
Mitotic Spindle 0.000507 0.000886 0.002871 0.021735 0.001982 0.007549 0.005354 0.055695 0.001418 0.006950 0.004214 0.026000
None 0.003599 0.001599 0.004364 0.002559 0.005390 0.002553 0.005715 0.003606 0.004705 0.002467 0.005095 0.002691
Nuclear Periphery 0.000351 0.000384 0.001998 0.000692 0.000289 0.000352 0.000890 0.000521 0.000313 0.000355 0.001399 0.000670
Nuclear Periphery Foci 0.001137 0.002827 0.004496 0.002295 0.001602 0.001027 0.002655 0.001523 0.001424 0.001188 0.003500 0.002198
Nucleolus 0.000813 0.000309 0.000309 0.000182 0.000757 0.000839 0.000530 0.000797 0.000778 0.000791 0.000429 0.000259
Nucleus 0.114359 0.087423 0.392254* 0.100995 0.130001* 0.083941 0.278213* 0.064821 0.124021 0.084254 0.330576* 0.096451
Peroxisomes 0.001626 0.004361 0.000235 0.000422 0.000826 0.002930 0.000313 0.003717 0.001132 0.003059 0.000277 0.000836
Vacuole 0.056587 0.116832 0.143601 0.208795* 0.039870 0.079796 0.142826 0.278447* 0.046261 0.083124 0.143182 0.217543*
Vacuole Periphery 0.001175 0.000502 0.000238 0.001931 0.000710 0.001120 0.000186 0.001242 0.000888 0.001064 0.000210 0.001844

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -4.21 -10.40 -2.56 -0.53 0.58 -2.92 -2.75 -3.29 0.96 1.42 -3.57 -7.10 -2.64 -1.33 -0.12
Bud Neck 0.44 0.70 4.45 2.76 3.63 -4.60 -0.86 -1.04 0.62 -0.61 -3.68 -0.67 1.52 4.34 1.57
Bud Site -2.44 -0.84 -2.75 1.12 -2.09 -6.12 -1.53 -2.77 -0.88 -2.19 -5.93 -1.91 -4.92 0.76 -3.05
Cell Periphery 3.19 -1.06 6.29 2.31 6.78 -0.77 -0.10 7.49 1.09 5.90 -0.75 -0.71 7.45 1.04 7.28
Cytoplasm 2.17 16.74 9.52 2.20 -9.43 3.63 18.30 13.37 11.66 1.56 0.66 25.30 20.19 17.28 -6.59
Cytoplasmic Foci 1.98 30.94 29.25 9.43 -6.87 -1.21 22.83 19.42 19.74 -1.06 4.60 37.19 36.00 25.74 -3.90
Eisosomes 4.13 4.18 11.23 2.93 5.99 -1.00 -4.36 2.77 2.79 5.69 2.08 -1.45 9.73 3.53 8.21
Endoplasmic Reticulum 0.71 -8.27 1.81 0.86 10.00 4.58 -5.27 3.45 -0.97 7.11 5.54 -9.93 1.25 -4.58 10.79
Endosome -1.41 5.89 7.94 3.73 2.60 -4.81 4.67 1.80 5.06 -0.52 -4.51 7.03 7.42 9.40 2.04
Golgi -0.55 4.87 1.83 1.16 -1.91 -3.19 7.52 4.47 3.97 -1.64 -2.47 6.92 0.81 2.61 -1.98
Lipid Particles 6.73 6.11 9.11 2.34 4.80 0.00 2.92 4.48 3.22 2.22 2.70 6.31 10.64 3.98 5.74
Mitochondria 3.40 2.23 2.74 -1.18 1.42 -2.81 2.24 2.11 3.15 0.35 -2.49 2.89 2.47 2.94 0.11
Mitotic Spindle -1.48 -3.56 -5.83 -5.63 -4.74 -2.81 -2.01 -2.94 -2.13 -1.87 -3.34 -2.53 -6.04 -3.48 -3.64
None 3.24 -0.02 1.60 -2.48 2.03 4.07 0.44 0.61 -2.47 0.24 4.46 0.58 3.39 -1.50 3.07
Nuclear Periphery -1.91 -17.73 -10.72 -7.51 8.78 -1.08 -14.09 -6.91 -5.24 1.99 -1.03 -22.45 -14.27 -9.38 6.32
Nuclear Periphery Foci -3.09 -7.65 -3.85 1.05 4.97 3.31 -5.46 -3.18 -4.97 2.02 1.16 -9.34 -4.14 -4.84 4.92
Nucleolus 3.80 3.91 4.56 1.92 2.41 0.11 1.03 1.41 2.16 1.35 0.21 1.84 2.49 2.69 3.32
Nucleus 0.42 -20.42 -0.69 -0.71 20.50 8.86 -13.32 5.13 0.70 14.37 8.20 -23.31 2.23 -5.25 23.82
Peroxisomes -1.72 9.79 9.42 2.88 -1.20 -4.28 3.29 0.07 3.36 -1.36 -4.18 7.33 6.18 6.38 -1.30
Vacuole -5.30 -12.36 -32.39 -11.29 -9.62 -12.27 -15.83 -16.76 -14.77 -8.17 -11.80 -20.28 -38.77 -31.33 -12.20
Vacuole Periphery 4.23 6.35 2.06 -0.35 -1.30 -1.68 4.79 3.36 4.00 -0.71 -0.72 7.70 1.53 1.79 -1.42
Standard name
Human Ortholog
Description Evolutionarily conserved 19S regulatory particle assembly-chaperone; proteasome-interacting protein involved in the assembly of the base subcomplex of the 19S proteasome regulatory particle (RP); null mutants accumulate ubiquitinated Gcn4p and display decreased 26S proteasome stability; interacts with Rpt5p
Localization
Cell Percentages cytoplasm (92%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Rpn14

Rpn14


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rpn14-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available