Standard name
Human Ortholog
Description Vacuolar Ca2+ ATPase involved in depleting cytosol of Ca2+ ions; prevents growth inhibition by activation of calcineurin in the presence of elevated concentrations of calcium; similar to mammalian PMCA1a
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Cytoplasm 0.14 0.13 0.09 0.25 0.15 0.19 0.15 0.08 0.13 0.11 0.18 0.12 0.34 0.35 0.42 – – – 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Endosome 0.06 0 0.19 0.16 0.15 0 0 0 0 0 0 0 0.07 0.06 0.07 – – – 0.07 0.08 0.13 0.11 0.15 0.13
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Mitochondria 0 0.06 0.15 0.26 0.14 0.36 0.38 0.41 0.49 0.47 0.55 0.46 0 0 0 – – – 0 0 0 0.05 0 0
Nucleus 0.1 0.07 0 0 0 0 0 0 0 0 0 0 0.05 0.06 0.05 – – – 0 0 0 0 0 0
Nuclear Periphery 0 0 0.05 0 0 0.08 0.07 0.12 0.12 0.11 0 0.1 0 0 0 – – – 0 0 0.09 0.05 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Vac/Vac Membrane 0.8 0.82 0.76 0.62 0.77 0.57 0.58 0.55 0.49 0.48 0.38 0.42 0.67 0.65 0.62 – – – 0.81 0.8 0.7 0.71 0.7 0.75
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 – – – 2 1 1 0 0 1
Bud 0 0 1 0 0 0 0 1 1 1 2 1 0 0 0 – – – 3 2 1 3 0 2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 – – – 0 0 1 0 0 0
Bud Site 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 – – – – – – – – –
Cell Periphery 0 0 0 0 0 2 1 7 6 5 5 13 0 0 0 – – – 0 0 0 0 0 0
Cytoplasm 46 25 25 45 36 69 57 32 49 39 58 43 44 44 63 – – – 3 3 2 7 0 3
Endoplasmic Reticulum 2 1 3 1 1 9 2 15 9 13 9 6 3 4 2 – – – 0 0 1 0 2 0
Endosome 19 5 55 29 37 7 12 10 0 0 0 1 9 8 10 – – – 27 17 39 25 27 28
Golgi 0 1 1 0 2 2 3 8 4 4 0 4 0 0 0 – – – 4 3 1 0 1 0
Mitochondria 7 12 43 47 34 127 143 165 177 163 179 159 0 0 0 – – – 3 3 6 10 6 8
Nucleus 33 13 6 1 9 2 4 4 6 2 2 3 7 7 7 – – – 11 5 1 1 4 2
Nuclear Periphery 2 6 13 0 3 29 25 48 42 37 13 34 0 0 0 – – – 9 3 26 10 7 4
Nucleolus 4 1 5 3 1 2 2 1 1 3 2 3 0 0 0 – – – 0 0 0 0 1 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 1 0 1 0
SpindlePole 1 1 0 1 0 0 0 1 0 0 0 0 0 0 0 – – – 1 0 0 1 1 0
Vac/Vac Membrane 263 160 214 111 189 202 218 223 177 167 124 147 86 83 92 – – – 299 165 205 165 128 171
Unique Cell Count 327 194 283 180 246 356 379 405 364 348 325 347 129 127 149 368 207 294 232 184 227
Labelled Cell Count 377 225 366 238 312 451 467 516 472 434 394 416 150 146 174 368 207 294 232 184 227


Vacuole Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.6 5.9 5.6 6.1 6.9 6.9 7.4 8.1 8.3 8.5 8.4 9.6 6.5 6.5 6.4 – – – 6.6 7.3 7.5
Std Deviation (1e-4) 1.1 1.0 1.3 1.8 1.6 1.7 1.8 1.9 1.8 2.3 2.5 3.0 1.6 1.6 0.9 – – – 2.7 3.5 2.8
Intensity Change (Log2) – – – 0.12 0.29 0.3 0.4 0.53 0.56 0.6 0.58 0.76 0.2 0.2 0.18 – – – 0.22 0.37 0.42


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Bud 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Cell Periphery 0 0 0 0 0 0 0 0 3.3 0 0 0 – – –
Cytoplasm 4.7 2.1 3.7 2.4 -0.4 1.8 1.0 3.2 1.4 6.4 6.5 8.2 – – –
Endoplasmic Reticulum 0 0 1.4 0 2.1 1.3 2.1 1.5 0 0 0 0 – – –
Endosome -0.9 -1.3 -7.4 -6.9 -7.5 -8.8 -8.6 -8.3 -8.4 -3.2 -3.4 -3.5 – – –
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Mitochondria 2.9 -0.4 5.8 6.4 7.2 8.9 8.4 10.2 8.2 -4.7 -4.6 -5.0 – – –
Nucleus 0 1.1 0 0 0 -0.4 0 0 0 0 0 0 – – –
Nuclear Periphery -2.9 -2.3 1.8 1.1 3.3 3.1 2.8 -0.4 2.5 0 0 0 – – –
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 – – –
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Vacuole -3.2 0.3 -5.0 -4.8 -5.5 -7.0 -7.1 -9.3 -8.4 -1.9 -2.2 -3.0 – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration – – – – – – – – – – – – – – – – – –
Actin 0.0005 0 0.0442 0.0315 0.1053 0.0042 0.0871 0.0001 0.0064 0.0019 0.009 0.0011 – – – – – –
Bud 0.0005 0.0005 0.0006 0.002 0.0007 0.0011 0.0013 0.0011 0.0003 0.001 0.0003 0.0004 – – – – – –
Bud Neck 0.0007 0.0002 0.0021 0.0013 0.0011 0.0002 0.0013 0.0004 0.0007 0.0042 0.0005 0.0008 – – – – – –
Bud Periphery 0.0013 0.0004 0.0011 0.0044 0.0015 0.003 0.002 0.0015 0.0008 0.0019 0.0003 0.0009 – – – – – –
Bud Site 0.0003 0.0013 0.0215 0.0023 0.0029 0.0003 0.0029 0.003 0.0011 0.0022 0.0014 0.0005 – – – – – –
Cell Periphery 0.0008 0.0005 0.0008 0.0007 0.0003 0.0006 0.0011 0.001 0.0007 0.0014 0.0001 0.0007 – – – – – –
Cytoplasm 0.004 0.0271 0.0049 0.004 0.0017 0.0023 0.0073 0.0108 0.0015 0.0031 0.0021 0.015 – – – – – –
Cytoplasmic Foci 0.0038 0.0047 0.0181 0.0056 0.0101 0.001 0.0258 0.0147 0.0072 0.0802 0.0734 0.0028 – – – – – –
Eisosomes 0 0 0.0003 0.0003 0.0004 0.0002 0.0003 0 0 0 0.0001 0 – – – – – –
Endoplasmic Reticulum 0.0129 0.0043 0.0015 0.0011 0.0013 0.0032 0.0047 0.0038 0.0038 0.0012 0.0014 0.0017 – – – – – –
Endosome 0.213 0.1018 0.1099 0.0936 0.0322 0.0719 0.1285 0.0962 0.1079 0.1012 0.2235 0.1081 – – – – – –
Golgi 0.0105 0.0039 0.0115 0.0172 0.0067 0.0049 0.0239 0.0032 0.0172 0.01 0.0653 0.0063 – – – – – –
Lipid Particles 0.0152 0.0006 0.0065 0.0088 0.0069 0.0011 0.021 0.0144 0.0181 0.0143 0.1107 0.0158 – – – – – –
Mitochondria 0.0344 0.0017 0.0521 0.0362 0.0025 0.0121 0.0149 0.0076 0.0204 0.0132 0.0197 0.0325 – – – – – –
None 0.0008 0.001 0.0017 0.0006 0.0031 0.0097 0.0052 0.0127 0.0003 0.0007 0.0003 0.0005 – – – – – –
Nuclear Periphery 0.1471 0.0269 0.009 0.0033 0.0043 0.0253 0.0234 0.0175 0.0216 0.0062 0.0098 0.0179 – – – – – –
Nucleolus 0.0015 0.0015 0.0009 0.0089 0.0004 0.0006 0.0076 0.002 0.0021 0.0073 0.0004 0.0027 – – – – – –
Nucleus 0.014 0.0176 0.0027 0.0476 0.001 0.0025 0.0115 0.0155 0.0057 0.0028 0.0007 0.0048 – – – – – –
Peroxisomes 0.0009 0.0001 0.0126 0.0025 0.0089 0.0001 0.0189 0.0005 0.0033 0.0188 0.0479 0.0007 – – – – – –
Punctate Nuclear 0.0011 0.0002 0.006 0.0078 0.0049 0.0001 0.0071 0.0003 0.0006 0.001 0.0017 0.0002 – – – – – –
Vacuole 0.2718 0.4525 0.3942 0.4258 0.3689 0.3803 0.3494 0.3885 0.3999 0.3746 0.1537 0.3576 – – – – – –
Vacuole Periphery 0.265 0.3532 0.2977 0.2945 0.4349 0.4752 0.2546 0.405 0.3803 0.3528 0.2775 0.4289 – – – – – –

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 19.8939 14.2831 20.3661 31.4675 16.4508 20.9788 24.2818 32.586 37.3614 26.9661
Translational Efficiency 0.5223 0.5406 0.581 0.4722 0.4999 0.6394 0.4324 0.3189 0.4077 0.3653

Hit Data

Dataset Hit
Protein Concentration –
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2046 913 2001 1754 600 1478 1149 2017 2646 2391 3150 3771

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 777.64 776.82 919.81 946.37 855.55 805.44 899.06 873.99 795.31 794.51 912.24 907.66
Standard Deviation 97.48 117.36 151.29 145.34 146.72 105.67 115.88 120.31 115.30 111.15 139.78 137.37
Intensity Change Log 2 — -0.001522 0.242233 0.283302 — -0.087075 0.071565 0.030765 — -0.045706 0.155350 0.156531

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000081 0.000084 0.000029 0.000221 0.000041 0.000110 0.000063 0.000065 0.000072 0.000100 0.000041 0.000137
Bud Neck 0.018886 0.008413 0.002785 0.007950 0.000923 0.005256 0.008370 0.006091 0.014813 0.006462 0.004822 0.006956
Bud Site 0.011457 0.013412 0.003897 0.016913 0.006543 0.003941 0.008541 0.014686 0.010343 0.007557 0.005591 0.015722
Cell Periphery 0.000085 0.000171 0.000058 0.000075 0.002744 0.000161 0.000050 0.000076 0.000688 0.000165 0.000055 0.000076
Cytoplasm 0.055998 0.050694 0.015748 0.040771 0.003000 0.013241 0.009811 0.024224 0.043980 0.027542 0.013583 0.031921
Cytoplasmic Foci 0.018367 0.079129 0.003024 0.018222 0.004040 0.032834 0.007728 0.016516 0.015118 0.050512 0.004740 0.017309
Eisosomes 0.000029 0.000026 0.000007 0.000009 0.000006 0.000022 0.000016 0.000009 0.000024 0.000024 0.000010 0.000009
Endoplasmic Reticulum 0.003791 0.003646 0.001386 0.002064 0.005000 0.004294 0.002544 0.001537 0.004065 0.004046 0.001808 0.001782
Endosome 0.066352 0.132738 0.012938 0.058951 0.014699 0.107299 0.041058 0.079050 0.054639 0.117013 0.023195 0.069702
Golgi 0.003015 0.006742 0.002145 0.005869 0.003985 0.005209 0.002800 0.007568 0.003235 0.005795 0.002384 0.006778
Lipid Particles 0.003074 0.003420 0.000203 0.000716 0.000245 0.004195 0.000490 0.000555 0.002433 0.003899 0.000307 0.000630
Mitochondria 0.007194 0.016039 0.003943 0.004756 0.012785 0.026098 0.004018 0.006847 0.008462 0.022257 0.003970 0.005875
Mitotic Spindle 0.007007 0.006858 0.002584 0.009287 0.000100 0.000742 0.003243 0.004284 0.005441 0.003077 0.002825 0.006611
None 0.000738 0.001036 0.000705 0.000321 0.000213 0.000316 0.000217 0.000227 0.000619 0.000591 0.000527 0.000271
Nuclear Periphery 0.003628 0.001031 0.003084 0.002334 0.000206 0.001539 0.002362 0.001304 0.002852 0.001345 0.002821 0.001783
Nuclear Periphery Foci 0.000637 0.000780 0.001610 0.000949 0.000108 0.000477 0.000696 0.000533 0.000517 0.000593 0.001277 0.000727
Nucleolus 0.007338 0.001915 0.005335 0.002116 0.000324 0.001500 0.009689 0.002230 0.005747 0.001659 0.006923 0.002177
Nucleus 0.084637 0.008347 0.012257 0.011139 0.011044 0.006895 0.024511 0.006051 0.067949 0.007449 0.016727 0.008418
Peroxisomes 0.001652 0.003138 0.000331 0.000769 0.000246 0.001710 0.001155 0.000917 0.001333 0.002255 0.000632 0.000848
Vacuole 0.649013* 0.472141* 0.319063* 0.710869* 0.175190 0.495442* 0.580131* 0.633292* 0.541570* 0.486545* 0.414290* 0.669375*
Vacuole Periphery 0.057023 0.190240* 0.608869* 0.105697* 0.758555* 0.288718* 0.292507* 0.193938* 0.216100* 0.251114* 0.493472* 0.152895*

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 0.08 4.95 -1.80 -1.79 -2.62 -0.86 -1.45 -0.79 0.52 0.04 -0.48 3.25 -1.59 -0.57 -2.52
Bud Neck 7.15 12.69 7.15 -0.73 -8.67 -5.07 -8.93 -6.86 -1.12 2.77 7.72 9.68 6.97 -1.69 -4.73
Bud Site -1.02 6.17 -2.91 -1.79 -8.34 2.11 -1.57 -5.01 -8.48 -3.78 2.69 4.50 -4.14 -7.47 -9.46
Cell Periphery -2.40 2.34 -0.01 2.37 -2.05 2.91 3.04 3.01 2.94 -2.37 2.57 3.12 3.00 3.77 -2.80
Cytoplasm 2.37 13.30 3.14 0.21 -9.77 -6.69 -4.93 -11.14 -5.46 -6.18 7.38 13.00 4.06 -3.98 -10.55
Cytoplasmic Foci -14.57 17.62 0.16 14.33 -12.48 -13.96 -4.04 -12.64 7.60 -7.88 -16.49 13.96 -2.22 15.41 -16.22
Eisosomes 1.06 16.33 14.43 7.74 -2.78 -7.40 -6.51 -2.51 6.79 6.11 -0.00 11.95 13.51 10.01 1.57
Endoplasmic Reticulum 0.36 11.55 7.71 3.87 -3.92 0.86 3.57 5.22 5.27 5.76 0.06 10.05 10.43 6.30 0.44
Endosome -11.95 21.72 2.58 13.33 -18.41 -19.80 -7.90 -17.69 6.15 -11.22 -17.50 14.33 -6.08 13.55 -22.65
Golgi -3.72 1.02 -2.41 1.13 -3.83 -0.94 1.00 -2.47 -2.20 -5.28 -3.51 1.22 -4.15 -0.96 -6.75
Lipid Particles -0.61 10.59 8.15 4.67 -5.37 -6.54 -2.64 -3.39 6.03 -0.78 -3.11 9.98 8.23 7.53 -5.52
Mitochondria -4.56 3.40 2.42 5.96 -0.80 -3.79 3.09 2.11 8.62 -3.43 -8.00 4.61 2.62 10.52 -3.22
Mitotic Spindle 0.19 3.27 -1.60 -1.46 -5.02 -3.67 -3.86 -7.69 -6.25 -0.71 2.12 2.37 -1.22 -3.99 -4.67
None -0.81 0.10 2.35 1.71 1.45 -1.14 -0.10 -0.26 1.44 -0.39 0.10 0.54 3.16 1.90 1.68
Nuclear Periphery 7.10 1.32 1.98 -4.63 0.57 -4.75 -5.83 -6.31 0.57 2.61 4.97 0.27 2.86 -2.62 2.51
Nuclear Periphery Foci -0.28 -2.08 -2.18 -1.05 1.17 -3.16 -6.52 -4.87 -0.50 1.33 -0.15 -2.50 -1.93 -1.44 1.72
Nucleolus 6.68 2.31 7.07 -0.84 5.07 -4.96 -8.66 -5.93 -2.02 6.62 7.14 -1.43 6.16 -2.20 8.20
Nucleus 19.81 18.84 18.50 -3.36 -0.77 1.06 -3.30 1.20 0.34 8.19 19.54 15.95 18.93 -2.51 5.77
Peroxisomes -1.92 5.36 3.62 3.12 -4.30 -5.68 -5.40 -4.37 3.01 1.24 -2.61 3.51 2.37 4.25 -2.22
Vacuole 16.09 40.49* -7.55 -22.32 -49.73* -31.97* -40.39 -49.61 -16.56 -6.26 6.16 15.53 -17.68 -27.66 -41.41
Vacuole Periphery -18.53* -80.28* -11.38 11.70 68.82* 39.26* 37.91* 50.68* 13.04 11.95 -4.58 -32.43 9.41 18.55 54.41*
Standard name
Human Ortholog
Description Vacuolar Ca2+ ATPase involved in depleting cytosol of Ca2+ ions; prevents growth inhibition by activation of calcineurin in the presence of elevated concentrations of calcium; similar to mammalian PMCA1a
Localization
Cell Percentages vacuolar membrane (44%)
Cell Cycle Regulation No
Subcompartmental Group vac memb-1

Pmc1

Pmc1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Pmc1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available