Standard name
Human Ortholog
Description Vacuolar Ca2+ ATPase involved in depleting cytosol of Ca2+ ions; prevents growth inhibition by activation of calcineurin in the presence of elevated concentrations of calcium; similar to mammalian PMCA1a
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Cytoplasm 0.14 0.13 0.09 0.25 0.15 0.19 0.15 0.08 0.13 0.11 0.18 0.12 0.34 0.35 0.42 – – – 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Endosome 0.06 0 0.19 0.16 0.15 0 0 0 0 0 0 0 0.07 0.06 0.07 – – – 0.07 0.08 0.13 0.11 0.15 0.13
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Mitochondria 0 0.06 0.15 0.26 0.14 0.36 0.38 0.41 0.49 0.47 0.55 0.46 0 0 0 – – – 0 0 0 0.05 0 0
Nucleus 0.1 0.07 0 0 0 0 0 0 0 0 0 0 0.05 0.06 0.05 – – – 0 0 0 0 0 0
Nuclear Periphery 0 0 0.05 0 0 0.08 0.07 0.12 0.12 0.11 0 0.1 0 0 0 – – – 0 0 0.09 0.05 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Vac/Vac Membrane 0.8 0.82 0.76 0.62 0.77 0.57 0.58 0.55 0.49 0.48 0.38 0.42 0.67 0.65 0.62 – – – 0.81 0.8 0.7 0.71 0.7 0.75
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 – – – 2 1 1 0 0 1
Bud 0 0 1 0 0 0 0 1 1 1 2 1 0 0 0 – – – 3 2 1 3 0 2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 – – – 0 0 1 0 0 0
Bud Site 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 – – – – – – – – –
Cell Periphery 0 0 0 0 0 2 1 7 6 5 5 13 0 0 0 – – – 0 0 0 0 0 0
Cytoplasm 46 25 25 45 36 69 57 32 49 39 58 43 44 44 63 – – – 3 3 2 7 0 3
Endoplasmic Reticulum 2 1 3 1 1 9 2 15 9 13 9 6 3 4 2 – – – 0 0 1 0 2 0
Endosome 19 5 55 29 37 7 12 10 0 0 0 1 9 8 10 – – – 27 17 39 25 27 28
Golgi 0 1 1 0 2 2 3 8 4 4 0 4 0 0 0 – – – 4 3 1 0 1 0
Mitochondria 7 12 43 47 34 127 143 165 177 163 179 159 0 0 0 – – – 3 3 6 10 6 8
Nucleus 33 13 6 1 9 2 4 4 6 2 2 3 7 7 7 – – – 11 5 1 1 4 2
Nuclear Periphery 2 6 13 0 3 29 25 48 42 37 13 34 0 0 0 – – – 9 3 26 10 7 4
Nucleolus 4 1 5 3 1 2 2 1 1 3 2 3 0 0 0 – – – 0 0 0 0 1 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 1 0 1 0
SpindlePole 1 1 0 1 0 0 0 1 0 0 0 0 0 0 0 – – – 1 0 0 1 1 0
Vac/Vac Membrane 263 160 214 111 189 202 218 223 177 167 124 147 86 83 92 – – – 299 165 205 165 128 171
Unique Cell Count 327 194 283 180 246 356 379 405 364 348 325 347 129 127 149 368 207 294 232 184 227
Labelled Cell Count 377 225 366 238 312 451 467 516 472 434 394 416 150 146 174 368 207 294 232 184 227


Vacuole Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.6 5.9 5.6 6.1 6.9 6.9 7.4 8.1 8.3 8.5 8.4 9.6 6.5 6.5 6.4 – – – 6.6 7.3 7.5
Std Deviation (1e-4) 1.1 1.0 1.3 1.8 1.6 1.7 1.8 1.9 1.8 2.3 2.5 3.0 1.6 1.6 0.9 – – – 2.7 3.5 2.8
Intensity Change (Log2) – – – 0.12 0.29 0.3 0.4 0.53 0.56 0.6 0.58 0.76 0.2 0.2 0.18 – – – 0.22 0.37 0.42


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Bud 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Cell Periphery 0 0 0 0 0 0 0 0 3.3 0 0 0 – – –
Cytoplasm 4.7 2.1 3.7 2.4 -0.4 1.8 1.0 3.2 1.4 6.4 6.5 8.2 – – –
Endoplasmic Reticulum 0 0 1.4 0 2.1 1.3 2.1 1.5 0 0 0 0 – – –
Endosome -0.9 -1.3 -7.4 -6.9 -7.5 -8.8 -8.6 -8.3 -8.4 -3.2 -3.4 -3.5 – – –
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Mitochondria 2.9 -0.4 5.8 6.4 7.2 8.9 8.4 10.2 8.2 -4.7 -4.6 -5.0 – – –
Nucleus 0 1.1 0 0 0 -0.4 0 0 0 0 0 0 – – –
Nuclear Periphery -2.9 -2.3 1.8 1.1 3.3 3.1 2.8 -0.4 2.5 0 0 0 – – –
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 – – –
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Vacuole -3.2 0.3 -5.0 -4.8 -5.5 -7.0 -7.1 -9.3 -8.4 -1.9 -2.2 -3.0 – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration – – – – – – – – – – – – – – – – – –
Actin 0.0005 0 0.0442 0.0315 0.1053 0.0042 0.0871 0.0001 0.0064 0.0019 0.009 0.0011 – – – – – –
Bud 0.0005 0.0005 0.0006 0.002 0.0007 0.0011 0.0013 0.0011 0.0003 0.001 0.0003 0.0004 – – – – – –
Bud Neck 0.0007 0.0002 0.0021 0.0013 0.0011 0.0002 0.0013 0.0004 0.0007 0.0042 0.0005 0.0008 – – – – – –
Bud Periphery 0.0013 0.0004 0.0011 0.0044 0.0015 0.003 0.002 0.0015 0.0008 0.0019 0.0003 0.0009 – – – – – –
Bud Site 0.0003 0.0013 0.0215 0.0023 0.0029 0.0003 0.0029 0.003 0.0011 0.0022 0.0014 0.0005 – – – – – –
Cell Periphery 0.0008 0.0005 0.0008 0.0007 0.0003 0.0006 0.0011 0.001 0.0007 0.0014 0.0001 0.0007 – – – – – –
Cytoplasm 0.004 0.0271 0.0049 0.004 0.0017 0.0023 0.0073 0.0108 0.0015 0.0031 0.0021 0.015 – – – – – –
Cytoplasmic Foci 0.0038 0.0047 0.0181 0.0056 0.0101 0.001 0.0258 0.0147 0.0072 0.0802 0.0734 0.0028 – – – – – –
Eisosomes 0 0 0.0003 0.0003 0.0004 0.0002 0.0003 0 0 0 0.0001 0 – – – – – –
Endoplasmic Reticulum 0.0129 0.0043 0.0015 0.0011 0.0013 0.0032 0.0047 0.0038 0.0038 0.0012 0.0014 0.0017 – – – – – –
Endosome 0.213 0.1018 0.1099 0.0936 0.0322 0.0719 0.1285 0.0962 0.1079 0.1012 0.2235 0.1081 – – – – – –
Golgi 0.0105 0.0039 0.0115 0.0172 0.0067 0.0049 0.0239 0.0032 0.0172 0.01 0.0653 0.0063 – – – – – –
Lipid Particles 0.0152 0.0006 0.0065 0.0088 0.0069 0.0011 0.021 0.0144 0.0181 0.0143 0.1107 0.0158 – – – – – –
Mitochondria 0.0344 0.0017 0.0521 0.0362 0.0025 0.0121 0.0149 0.0076 0.0204 0.0132 0.0197 0.0325 – – – – – –
None 0.0008 0.001 0.0017 0.0006 0.0031 0.0097 0.0052 0.0127 0.0003 0.0007 0.0003 0.0005 – – – – – –
Nuclear Periphery 0.1471 0.0269 0.009 0.0033 0.0043 0.0253 0.0234 0.0175 0.0216 0.0062 0.0098 0.0179 – – – – – –
Nucleolus 0.0015 0.0015 0.0009 0.0089 0.0004 0.0006 0.0076 0.002 0.0021 0.0073 0.0004 0.0027 – – – – – –
Nucleus 0.014 0.0176 0.0027 0.0476 0.001 0.0025 0.0115 0.0155 0.0057 0.0028 0.0007 0.0048 – – – – – –
Peroxisomes 0.0009 0.0001 0.0126 0.0025 0.0089 0.0001 0.0189 0.0005 0.0033 0.0188 0.0479 0.0007 – – – – – –
Punctate Nuclear 0.0011 0.0002 0.006 0.0078 0.0049 0.0001 0.0071 0.0003 0.0006 0.001 0.0017 0.0002 – – – – – –
Vacuole 0.2718 0.4525 0.3942 0.4258 0.3689 0.3803 0.3494 0.3885 0.3999 0.3746 0.1537 0.3576 – – – – – –
Vacuole Periphery 0.265 0.3532 0.2977 0.2945 0.4349 0.4752 0.2546 0.405 0.3803 0.3528 0.2775 0.4289 – – – – – –

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 19.8939 14.2831 20.3661 31.4675 16.4508 20.9788 24.2818 32.586 37.3614 26.9661
Translational Efficiency 0.5223 0.5406 0.581 0.4722 0.4999 0.6394 0.4324 0.3189 0.4077 0.3653

Hit Data

Dataset Hit
Protein Concentration –
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Vacuolar Ca2+ ATPase involved in depleting cytosol of Ca2+ ions; prevents growth inhibition by activation of calcineurin in the presence of elevated concentrations of calcium; similar to mammalian PMCA1a
Localization
Cell Percentages vacuolar membrane (44%)
Cell Cycle Regulation No
Subcompartmental Group vac memb-1

Pmc1

Pmc1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Pmc1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available