Standard name | |
---|---|
Human Ortholog | |
Description | Protein involved in metabolism of phytosphingosine; not an essential gene |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 1 | 5 | 0 | 1 | 1 | 5 | 3 | 2 | 3 | 1 | 2 | 2 | 0 | 1 | 4 | 8 | 7 | 7 | 1 | 11 | 5 | 4 | 10 | 12 |
Bud | 1 | 3 | 1 | 4 | 9 | 7 | 15 | 19 | 18 | 27 | 20 | 27 | 2 | 2 | 1 | 2 | 0 | 0 | 2 | 4 | 1 | 3 | 11 | 14 |
Bud Neck | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 11 | 3 | 4 | 10 | 5 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 3 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 5 | 5 | 3 | 14 | 9 | 15 | 12 | 11 | 13 | 14 | 26 | 9 | 14 | 21 | 13 | 17 | 20 | 0 | 4 | 3 | 1 | 3 | 11 |
Cytoplasm | 69 | 240 | 200 | 233 | 251 | 170 | 239 | 327 | 205 | 301 | 187 | 270 | 236 | 328 | 310 | 118 | 86 | 122 | 37 | 138 | 56 | 65 | 119 | 141 |
Endoplasmic Reticulum | 43 | 170 | 40 | 17 | 126 | 138 | 122 | 83 | 67 | 50 | 53 | 59 | 81 | 86 | 123 | 4 | 1 | 3 | 90 | 297 | 213 | 137 | 148 | 181 |
Endosome | 1 | 0 | 5 | 7 | 17 | 8 | 14 | 9 | 5 | 4 | 4 | 4 | 0 | 0 | 0 | 2 | 6 | 4 | 1 | 3 | 2 | 2 | 5 | 5 |
Golgi | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 1 | 0 | 0 | 2 | 9 |
Mitochondria | 8 | 2 | 26 | 50 | 127 | 118 | 242 | 234 | 252 | 255 | 212 | 377 | 0 | 0 | 1 | 6 | 4 | 5 | 12 | 29 | 19 | 47 | 52 | 44 |
Nucleus | 0 | 3 | 0 | 1 | 2 | 5 | 7 | 10 | 14 | 5 | 7 | 5 | 2 | 1 | 0 | 7 | 2 | 3 | 0 | 1 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 3 | 5 | 14 | 33 | 3 | 8 | 9 | 9 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 1 | 1 | 2 | 1 | 0 |
Nucleolus | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 1 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 1 | 1 | 0 | 1 | 2 | 1 | 2 |
Vac/Vac Membrane | 9 | 27 | 16 | 24 | 73 | 41 | 92 | 146 | 117 | 132 | 52 | 76 | 5 | 9 | 23 | 13 | 22 | 15 | 3 | 25 | 4 | 18 | 30 | 49 |
Unique Cell Count | 123 | 420 | 277 | 303 | 526 | 416 | 624 | 739 | 549 | 641 | 453 | 678 | 323 | 418 | 449 | 159 | 137 | 170 | 157 | 535 | 317 | 293 | 404 | 484 |
Labelled Cell Count | 133 | 457 | 294 | 342 | 623 | 507 | 763 | 877 | 697 | 802 | 561 | 858 | 337 | 442 | 483 | 176 | 148 | 181 | 157 | 535 | 317 | 293 | 404 | 484 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.3 | 6.1 | 4.8 | 4.7 | 5.7 | 5.9 | 5.2 | 5.2 | 5.0 | 4.9 | 5.1 | 4.9 | 6.3 | 6.5 | 6.7 | 7.8 | 8.4 | 8.3 | 7.0 | 6.8 | 7.3 |
Std Deviation (1e-4) | 0.7 | 0.9 | 1.2 | 1.4 | 1.4 | 1.2 | 1.4 | 1.2 | 1.4 | 1.2 | 1.5 | 1.3 | 1.0 | 1.0 | 1.1 | 1.4 | 1.7 | 1.5 | 2.3 | 1.7 | 1.8 |
Intensity Change (Log2) | – | – | – | -0.03 | 0.25 | 0.29 | 0.09 | 0.1 | 0.05 | 0.01 | 0.08 | 0.02 | 0.38 | 0.42 | 0.47 | 0.68 | 0.79 | 0.78 | 0.53 | 0.49 | 0.59 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 2.3 | 2.6 | 0 | 3.2 | 3.0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0.8 | 0.3 | 0.6 | 0 | 0.2 | 0 | 1.1 | 1.6 | 0.8 | 1.2 | 2.0 | 3.2 | 4.5 | 4.4 |
Cytoplasm | 1.3 | -6.6 | -8.1 | -9.4 | -7.9 | -9.5 | -7.1 | -8.1 | -9.1 | 0.2 | 1.9 | -0.9 | 0.5 | -2.0 | -0.1 |
Endoplasmic Reticulum | 0 | 3.2 | 5.5 | 1.8 | -1.4 | -0.9 | 0 | -1.1 | -2.6 | 3.2 | 2.1 | 4.1 | -4.0 | -4.4 | -4.4 |
Endosome | 0 | 1.2 | 0.1 | 0.4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 5.1 | 6.0 | 8.9 | 7.2 | 10.5 | 0 | 10.5 | 13.1 | -5.6 | -6.4 | -6.3 | -2.2 | -2.4 | -2.6 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 3.6 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 1.0 | 3.5 | 1.9 | 3.8 | 5.4 | 5.7 | 5.6 | 2.6 | 2.6 | -2.8 | -2.5 | -0.4 | 1.0 | 3.4 | 1.2 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.7626 | 2.7813 | 2.8034 | 2.7218 | 2.4271 | 2.6884 | 2.8371 | 2.8259 | 3.1552 | 2.7304 | 2.7951 | 2.9588 | 0.91 | 0.9897 | 0.6015 | 1.5072 | 0.6351 | 0.9011 |
Actin | 0.006 | 0.0018 | 0.0106 | 0.0217 | 0.0249 | 0.0093 | 0.061 | 0.0039 | 0.0066 | 0.0021 | 0.0281 | 0.0018 | 0.0177 | 0.0005 | 0.01 | 0.0072 | 0.0032 | 0.0011 |
Bud | 0.0002 | 0.0004 | 0.0003 | 0.0004 | 0.0004 | 0.0001 | 0.0012 | 0.0005 | 0.0005 | 0.0001 | 0.0001 | 0.0001 | 0.0004 | 0.0003 | 0.0001 | 0.0003 | 0.001 | 0 |
Bud Neck | 0.0066 | 0.0007 | 0.0012 | 0.0011 | 0.0008 | 0.0035 | 0.0242 | 0.0004 | 0.0023 | 0.0007 | 0.0005 | 0.0015 | 0.0014 | 0.0001 | 0.0005 | 0.0014 | 0.0058 | 0.0008 |
Bud Periphery | 0.0005 | 0.0006 | 0.0003 | 0.0007 | 0.0005 | 0.0002 | 0.0019 | 0.0008 | 0.0009 | 0.0002 | 0.0002 | 0.0002 | 0.0005 | 0.0001 | 0.0002 | 0.0004 | 0.0014 | 0.0001 |
Bud Site | 0.0008 | 0.019 | 0.0038 | 0.0021 | 0.0021 | 0.0003 | 0.0236 | 0.0053 | 0.0034 | 0.0002 | 0.0003 | 0.0002 | 0.0035 | 0.0071 | 0.0031 | 0.0011 | 0.0263 | 0 |
Cell Periphery | 0.0008 | 0.002 | 0.0006 | 0.0004 | 0.0001 | 0.0004 | 0.0028 | 0.0016 | 0.002 | 0.0019 | 0.0009 | 0.0008 | 0.0004 | 0.0007 | 0.001 | 0.0009 | 0.001 | 0.0002 |
Cytoplasm | 0.3614 | 0.4458 | 0.4298 | 0.3871 | 0.3894 | 0.4004 | 0.2697 | 0.3952 | 0.3903 | 0.4833 | 0.4193 | 0.4243 | 0.2619 | 0.4126 | 0.4 | 0.3556 | 0.3122 | 0.3213 |
Cytoplasmic Foci | 0.0085 | 0.0091 | 0.025 | 0.0104 | 0.0226 | 0.0111 | 0.0139 | 0.0129 | 0.0104 | 0.0099 | 0.042 | 0.0095 | 0.036 | 0.0062 | 0.007 | 0.0136 | 0.0156 | 0.0048 |
Eisosomes | 0.0003 | 0.0002 | 0.0001 | 0.0003 | 0.0002 | 0.0001 | 0.0007 | 0.0002 | 0.0011 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0 | 0.0003 | 0.0001 | 0.0001 | 0.0001 |
Endoplasmic Reticulum | 0.3828 | 0.3085 | 0.3021 | 0.3386 | 0.1931 | 0.3946 | 0.3043 | 0.3136 | 0.3336 | 0.2393 | 0.3154 | 0.3122 | 0.521 | 0.4562 | 0.4506 | 0.4686 | 0.4397 | 0.5628 |
Endosome | 0.0257 | 0.0182 | 0.0296 | 0.0362 | 0.0486 | 0.0174 | 0.0511 | 0.0247 | 0.0149 | 0.0129 | 0.0109 | 0.0104 | 0.0334 | 0.0117 | 0.011 | 0.0508 | 0.0304 | 0.0087 |
Golgi | 0.0019 | 0.0014 | 0.0027 | 0.006 | 0.0104 | 0.0018 | 0.0138 | 0.0037 | 0.0046 | 0.0011 | 0.0037 | 0.0015 | 0.0081 | 0.0006 | 0.0013 | 0.013 | 0.0048 | 0.0006 |
Lipid Particles | 0.0065 | 0.0038 | 0.0058 | 0.0066 | 0.0257 | 0.0026 | 0.0252 | 0.0082 | 0.0077 | 0.0028 | 0.005 | 0.0063 | 0.0083 | 0.0069 | 0.0052 | 0.0185 | 0.0066 | 0.0032 |
Mitochondria | 0.0019 | 0.0009 | 0.0018 | 0.0036 | 0.005 | 0.0011 | 0.0063 | 0.0022 | 0.0031 | 0.0006 | 0.0015 | 0.0008 | 0.0143 | 0.0002 | 0.0003 | 0.0075 | 0.0048 | 0.0161 |
None | 0.1265 | 0.1312 | 0.1252 | 0.1292 | 0.0969 | 0.0966 | 0.1478 | 0.1955 | 0.1766 | 0.2296 | 0.1511 | 0.2082 | 0.0555 | 0.0766 | 0.0876 | 0.0236 | 0.0639 | 0.0638 |
Nuclear Periphery | 0.0356 | 0.0256 | 0.0222 | 0.0295 | 0.1012 | 0.0279 | 0.02 | 0.0102 | 0.0208 | 0.0076 | 0.0064 | 0.0058 | 0.0152 | 0.0108 | 0.0103 | 0.0124 | 0.0555 | 0.0084 |
Nucleolus | 0.0013 | 0.0014 | 0.0011 | 0.001 | 0.0011 | 0.0005 | 0.0051 | 0.0004 | 0.0005 | 0.0001 | 0.0001 | 0.0001 | 0.0004 | 0.0001 | 0.0001 | 0.0002 | 0.004 | 0.0001 |
Nucleus | 0.0167 | 0.0136 | 0.0151 | 0.0085 | 0.0151 | 0.015 | 0.006 | 0.0041 | 0.0109 | 0.0021 | 0.0024 | 0.0025 | 0.0024 | 0.003 | 0.0024 | 0.0032 | 0.0072 | 0.0028 |
Peroxisomes | 0.0008 | 0.0002 | 0.0023 | 0.0013 | 0.0254 | 0.0024 | 0.0042 | 0.0004 | 0.0008 | 0.0001 | 0.0049 | 0.0093 | 0.0089 | 0.0001 | 0.0048 | 0.0124 | 0.0066 | 0.0007 |
Punctate Nuclear | 0.0059 | 0.0062 | 0.0138 | 0.008 | 0.029 | 0.009 | 0.0075 | 0.0093 | 0.003 | 0.0007 | 0.0044 | 0.001 | 0.0046 | 0.0009 | 0.0008 | 0.0009 | 0.0028 | 0.0008 |
Vacuole | 0.0074 | 0.0085 | 0.0056 | 0.0055 | 0.0054 | 0.0047 | 0.0075 | 0.0062 | 0.0049 | 0.0042 | 0.0023 | 0.0032 | 0.0046 | 0.0051 | 0.003 | 0.0071 | 0.0044 | 0.0033 |
Vacuole Periphery | 0.0019 | 0.0008 | 0.0009 | 0.0019 | 0.0019 | 0.0009 | 0.0022 | 0.0006 | 0.0011 | 0.0005 | 0.0003 | 0.0003 | 0.0012 | 0.0003 | 0.0003 | 0.0013 | 0.0027 | 0.0003 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 14.7121 | 16.9093 | 8.365 | 22.5641 | 9.5374 | 18.905 | 24.6956 | 22.4375 | 14.7239 | 14.079 |
Translational Efficiency | 0.9256 | 0.8747 | 1.7096 | 0.7419 | 1.2581 | 1.1056 | 0.7622 | 0.9693 | 1.5283 | 1.0896 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1614 | 1562 | 139 | 978 | 138 | 1251 | 861 | 1265 | 1752 | 2813 | 1000 | 2243 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 608.97 | 770.35 | 1020.80 | 1076.29 | 748.20 | 685.74 | 929.13 | 1008.97 | 619.94 | 732.72 | 941.87 | 1038.32 |
Standard Deviation | 79.25 | 111.25 | 100.51 | 174.15 | 98.10 | 100.74 | 119.40 | 167.56 | 89.16 | 114.69 | 121.18 | 173.70 |
Intensity Change Log 2 | — | 0.339143 | 0.745257 | 0.821624 | — | -0.125762 | 0.312457 | 0.431387 | — | 0.101498 | 0.522821 | 0.619626 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000157 | 0.000376 | 0.000692 | 0.003179 | 0.000257 | 0.000499 | 0.000505 | 0.002945 | 0.000165 | 0.000430 | 0.000531 | 0.003047 |
Bud Neck | 0.007601 | 0.009157 | 0.002607 | 0.007529 | 0.002857 | 0.022896 | 0.012451 | 0.008106 | 0.007227 | 0.015267 | 0.011083 | 0.007854 |
Bud Site | 0.005083 | 0.010192 | 0.010817 | 0.030337 | 0.002962 | 0.013049 | 0.010794 | 0.021747 | 0.004916 | 0.011463 | 0.010797 | 0.025492 |
Cell Periphery | 0.000693 | 0.000370 | 0.000679 | 0.003534 | 0.000692 | 0.000630 | 0.000684 | 0.002076 | 0.000693 | 0.000486 | 0.000683 | 0.002712 |
Cytoplasm | 0.384934* | 0.422761* | 0.215430* | 0.195454* | 0.385668* | 0.290113* | 0.390848* | 0.250545* | 0.384992* | 0.363769* | 0.366465* | 0.226524* |
Cytoplasmic Foci | 0.151778 | 0.192205* | 0.059059 | 0.029855 | 0.249134* | 0.223710* | 0.019858 | 0.050197 | 0.159447 | 0.206216* | 0.025307 | 0.041327 |
Eisosomes | 0.000689 | 0.000327 | 0.000188 | 0.000405 | 0.000380 | 0.000744 | 0.000143 | 0.000790 | 0.000664 | 0.000512 | 0.000149 | 0.000622 |
Endoplasmic Reticulum | 0.142449* | 0.075360 | 0.140948* | 0.466543* | 0.063806 | 0.106111* | 0.201247* | 0.389054* | 0.136255* | 0.089036 | 0.192865* | 0.422841* |
Endosome | 0.008921 | 0.016725 | 0.023159 | 0.012217 | 0.022713 | 0.032192 | 0.006752 | 0.014569 | 0.010008 | 0.023604 | 0.009033 | 0.013543 |
Golgi | 0.009674 | 0.005184 | 0.000315 | 0.002373 | 0.003394 | 0.020992 | 0.000515 | 0.004405 | 0.009180 | 0.012215 | 0.000487 | 0.003519 |
Lipid Particles | 0.120505* | 0.041146 | 0.022456 | 0.034143 | 0.072178 | 0.097576 | 0.015296 | 0.041747 | 0.116699* | 0.066241 | 0.016292 | 0.038431 |
Mitochondria | 0.013464 | 0.005085 | 0.000437 | 0.000585 | 0.000920 | 0.027434 | 0.001318 | 0.001088 | 0.012476 | 0.015024 | 0.001196 | 0.000868 |
Mitotic Spindle | 0.000077 | 0.001204 | 0.000207 | 0.005519 | 0.000061 | 0.001259 | 0.002193 | 0.002920 | 0.000076 | 0.001229 | 0.001916 | 0.004053 |
None | 0.007240 | 0.003498 | 0.001252 | 0.000643 | 0.001633 | 0.003542 | 0.001542 | 0.001119 | 0.006798 | 0.003517 | 0.001501 | 0.000912 |
Nuclear Periphery | 0.008338 | 0.004708 | 0.028674 | 0.002781 | 0.007379 | 0.005092 | 0.024518 | 0.003901 | 0.008263 | 0.004879 | 0.025096 | 0.003413 |
Nuclear Periphery Foci | 0.003785 | 0.006903 | 0.089158 | 0.056010 | 0.016978 | 0.003252 | 0.022280 | 0.040368 | 0.004824 | 0.005279 | 0.031576 | 0.047188 |
Nucleolus | 0.000929 | 0.001266 | 0.001114 | 0.000138 | 0.001832 | 0.002259 | 0.000352 | 0.000350 | 0.001000 | 0.001708 | 0.000458 | 0.000257 |
Nucleus | 0.100426 | 0.128957 | 0.198829* | 0.036209 | 0.106930 | 0.067726 | 0.214527* | 0.051661 | 0.100938 | 0.101726 | 0.212345* | 0.044923 |
Peroxisomes | 0.005121 | 0.003481 | 0.001061 | 0.000875 | 0.003173 | 0.009685 | 0.001078 | 0.001479 | 0.004968 | 0.006240 | 0.001076 | 0.001216 |
Vacuole | 0.026293 | 0.069060 | 0.202476* | 0.110975 | 0.055736 | 0.065714 | 0.072516 | 0.109489 | 0.028612 | 0.067572 | 0.090581 | 0.110137 |
Vacuole Periphery | 0.001843 | 0.002035 | 0.000445 | 0.000696 | 0.001314 | 0.005524 | 0.000583 | 0.001445 | 0.001801 | 0.003587 | 0.000564 | 0.001119 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -3.50 | -5.06 | -7.70 | -7.20 | -6.26 | -2.48 | -1.68 | -5.84 | -5.31 | -5.04 | -4.45 | -2.83 | -9.34 | -8.53 | -7.59 |
Bud Neck | -1.38 | 6.28 | -0.61 | 0.80 | -6.82 | -9.39 | -4.26 | -6.41 | 6.55 | 1.70 | -6.49 | -1.95 | -1.43 | 5.86 | 1.38 |
Bud Site | -2.74 | -1.03 | -7.32 | -5.77 | -3.59 | -5.55 | -3.93 | -7.86 | -3.39 | -3.93 | -4.30 | -2.93 | -9.54 | -6.68 | -5.98 |
Cell Periphery | 6.19 | 0.18 | -3.87 | -4.33 | -3.89 | 0.52 | 0.06 | -4.91 | -5.41 | -4.80 | 4.21 | 0.10 | -5.79 | -6.40 | -5.57 |
Cytoplasm | -4.17 | 9.24 | 19.53 | 22.70 | 0.72 | 4.84 | -0.20 | 6.73 | 4.02 | 12.42 | 2.83 | 2.14 | 20.22 | 19.26 | 14.31 |
Cytoplasmic Foci | -6.62 | 11.89 | 27.25 | 31.14 | 3.99 | 1.70 | 15.73 | 13.55 | 29.54 | -10.52 | -8.97 | 31.93 | 28.21 | 41.90 | -6.90 |
Eisosomes | 5.71 | 9.52 | 4.55 | -1.50 | -5.18 | -4.89 | 4.10 | -3.25 | -0.35 | -5.66 | 2.76 | 10.95 | 0.53 | -1.51 | -7.05 |
Endoplasmic Reticulum | 8.99 | 0.85 | -22.36 | -28.96 | -14.67 | -3.26 | -9.00 | -20.06 | -22.88 | -13.15 | 7.02 | -5.09 | -27.80 | -36.88 | -19.20 |
Endosome | -7.41 | -5.68 | -4.35 | 3.82 | 4.21 | -2.28 | 4.19 | 2.12 | 9.50 | -8.06 | -12.77 | 1.08 | -5.13 | 9.41 | -5.47 |
Golgi | 6.58 | 17.24 | 11.45 | 5.25 | -5.51 | -12.31 | 4.51 | -1.04 | 11.83 | -6.32 | -3.71 | 17.08 | 9.31 | 12.01 | -7.80 |
Lipid Particles | 18.77 | 23.25 | 18.96 | 1.81 | -4.52 | -2.47 | 5.66 | 2.92 | 12.84 | -10.38 | 12.24 | 26.42 | 19.61 | 10.38 | -11.40 |
Mitochondria | 6.73 | 11.53 | 11.38 | 8.33 | -1.74 | -12.26 | -1.93 | -0.78 | 12.18 | 1.15 | -1.72 | 10.75 | 11.07 | 13.74 | 2.29 |
Mitotic Spindle | -2.03 | -2.48 | -3.73 | -2.85 | -3.64 | -3.51 | -3.23 | -3.47 | -2.01 | -0.63 | -3.36 | -3.24 | -5.05 | -3.45 | -2.11 |
None | 3.83 | 6.26 | 7.23 | 6.95 | 1.42 | -2.19 | 0.28 | 0.91 | 3.73 | 1.05 | 3.63 | 6.12 | 6.98 | 6.97 | 1.87 |
Nuclear Periphery | 3.72 | -3.71 | 6.92 | 2.87 | 4.95 | 0.91 | -5.40 | 1.43 | 1.54 | 9.61 | 3.97 | -7.95 | 6.36 | 2.84 | 10.97 |
Nuclear Periphery Foci | -5.09 | -8.12 | -17.65 | -16.44 | 2.57 | 3.43 | -1.37 | -5.27 | -17.12 | -6.03 | -0.89 | -11.42 | -23.26 | -23.29 | -5.66 |
Nucleolus | -1.95 | -0.56 | 6.33 | 10.95 | 4.91 | -0.57 | 2.03 | 1.99 | 3.21 | -0.39 | -2.40 | 4.09 | 5.54 | 5.22 | 3.13 |
Nucleus | -6.40 | -5.18 | 16.51 | 21.96 | 8.45 | 3.43 | -7.98 | 4.66 | 4.19 | 22.93 | -0.16 | -16.16 | 16.49 | 19.51 | 25.48 |
Peroxisomes | 4.26 | 11.99 | 11.05 | 7.68 | 0.58 | -7.19 | 2.87 | 2.49 | 12.95 | -1.26 | -3.07 | 10.10 | 12.06 | 14.91 | -0.56 |
Vacuole | -14.48 | -10.65 | -16.93 | -9.05 | 5.11 | -1.26 | -2.89 | -7.64 | -9.37 | -6.20 | -15.66 | -14.26 | -24.06 | -13.06 | -3.66 |
Vacuole Periphery | -0.78 | 13.41 | 6.11 | 5.08 | -1.41 | -7.43 | 1.69 | -0.15 | 6.75 | -1.67 | -7.14 | 5.33 | 2.72 | 7.37 | -1.59 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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