Standard name | |
---|---|
Human Ortholog | |
Description | Karyopherin beta; responsible for import of the Toa1p-Toa2p complex into the nucleus; binds to nucleoporins Nup1p and Nup2p; may play a role in regulation of pleiotropic drug resistance |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 0 | 1 | 0 |
Bud | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 8 | 2 | 2 | 7 | 7 | 0 | 0 | 0 | 2 | 1 | 0 | 3 | 5 | 3 | 2 | 9 | 6 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 3 | 8 | 5 | 5 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 20 | 16 | 10 | 24 | 21 | 55 | 68 | 71 | 46 | 74 | 94 | 105 | 5 | 35 | 32 | 86 | 76 | 55 | 6 | 5 | 3 | 6 | 14 | 16 |
Endoplasmic Reticulum | 4 | 1 | 0 | 2 | 0 | 2 | 1 | 0 | 2 | 2 | 1 | 1 | 2 | 0 | 6 | 20 | 16 | 21 | 2 | 3 | 6 | 1 | 11 | 7 |
Endosome | 1 | 1 | 10 | 11 | 17 | 12 | 11 | 24 | 5 | 5 | 4 | 17 | 0 | 1 | 2 | 7 | 9 | 13 | 10 | 7 | 16 | 9 | 37 | 45 |
Golgi | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 3 | 0 | 1 | 4 | 4 |
Mitochondria | 3 | 7 | 2 | 17 | 4 | 71 | 131 | 207 | 123 | 261 | 338 | 346 | 0 | 0 | 0 | 26 | 12 | 7 | 4 | 1 | 2 | 5 | 18 | 13 |
Nucleus | 53 | 23 | 17 | 36 | 34 | 52 | 77 | 92 | 49 | 98 | 113 | 112 | 5 | 13 | 21 | 135 | 97 | 58 | 58 | 32 | 22 | 11 | 19 | 13 |
Nuclear Periphery | 38 | 15 | 51 | 55 | 34 | 32 | 49 | 46 | 26 | 45 | 30 | 50 | 1 | 3 | 0 | 80 | 42 | 34 | 215 | 75 | 199 | 48 | 68 | 36 |
Nucleolus | 3 | 1 | 2 | 1 | 1 | 4 | 1 | 15 | 3 | 5 | 4 | 7 | 0 | 0 | 0 | 1 | 3 | 4 | 9 | 3 | 4 | 1 | 3 | 2 |
Peroxisomes | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 3 | 2 | 3 | 0 | 0 | 0 | 0 | 0 | 1 | 7 | 2 | 7 | 7 | 8 | 7 |
Vac/Vac Membrane | 248 | 106 | 210 | 125 | 153 | 175 | 236 | 230 | 96 | 163 | 131 | 210 | 21 | 102 | 80 | 254 | 203 | 271 | 44 | 22 | 31 | 25 | 62 | 49 |
Unique Cell Count | 310 | 143 | 267 | 221 | 225 | 337 | 434 | 553 | 270 | 509 | 561 | 646 | 33 | 144 | 124 | 492 | 382 | 378 | 369 | 165 | 304 | 124 | 269 | 213 |
Labelled Cell Count | 370 | 170 | 304 | 273 | 265 | 405 | 574 | 695 | 355 | 667 | 730 | 864 | 34 | 155 | 141 | 612 | 460 | 466 | 369 | 165 | 304 | 124 | 269 | 213 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 7.8 | 7.9 | 8.0 | 7.6 | 7.3 | 5.8 | 5.5 | 5.4 | 5.1 | 4.7 | 4.7 | 4.8 | 7.4 | 8.3 | 8.0 | 6.9 | 7.7 | 9.8 | 8.3 | 8.8 | 9.5 |
Std Deviation (1e-4) | 1.5 | 1.6 | 1.4 | 1.9 | 1.7 | 1.4 | 1.3 | 1.3 | 1.3 | 1.2 | 1.2 | 1.3 | 1.0 | 1.3 | 1.2 | 1.3 | 1.7 | 2.3 | 1.3 | 1.4 | 1.7 |
Intensity Change (Log2) | – | – | – | -0.08 | -0.15 | -0.47 | -0.55 | -0.57 | -0.64 | -0.76 | -0.77 | -0.73 | -0.12 | 0.04 | -0.01 | -0.21 | -0.06 | 0.28 | 0.05 | 0.14 | 0.25 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 3.1 | 2.5 | 5.0 | 4.9 | 4.1 | 5.0 | 4.6 | 5.3 | 5.2 | 0 | 6.4 | 6.6 | 5.4 | 6.0 | 4.5 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.3 | 3.4 | 3.9 |
Endosome | 0.7 | 1.8 | -0.1 | -0.9 | 0.4 | -1.3 | 0 | 0 | 0 | 0 | 0 | 0 | -2.1 | -1.0 | -0.2 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 3.9 | 0 | 7.6 | 9.7 | 11.3 | 12.3 | 0 | 0 | 0 | 0 | 0 | 0 | 3.2 | 2.1 | 0 |
Nucleus | 3.5 | 3.2 | 3.5 | 4.3 | 4.1 | 4.2 | 4.8 | 5.1 | 4.3 | 0 | 1.0 | 3.3 | 6.9 | 6.3 | 3.5 |
Nuclear Periphery | 1.5 | -1.2 | -3.4 | -2.9 | -4.5 | -3.1 | 0 | 0 | 0 | -2.3 | -4.9 | -5.2 | -1.0 | -2.9 | -3.7 |
Nucleolus | 0 | 0 | 0 | 0 | 1.8 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -5.2 | -2.7 | -6.8 | -6.5 | -10.0 | -10.1 | -12.4 | -15.1 | -12.7 | -1.9 | -1.8 | -3.0 | -7.3 | -6.6 | -2.0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 4.3091 | 4.6517 | 4.1089 | 3.6392 | 3.363 | 3.7633 | 4.7774 | 5.8667 | 5.9138 | 5.4666 | 5.5725 | 5.1985 | 2.1485 | 5.8395 | 5.5855 | 6.1707 | 5.4584 | 5.4216 |
Actin | 0.0102 | 0.0005 | 0.0347 | 0.0039 | 0.0325 | 0.0009 | 0.035 | 0.0003 | 0.0039 | 0.0013 | 0.0022 | 0.0018 | 0.0045 | 0.0005 | 0.0014 | 0.0021 | 0.0004 | 0.0015 |
Bud | 0.0017 | 0.0001 | 0.0004 | 0.0001 | 0.0005 | 0 | 0.0004 | 0.0003 | 0.0001 | 0 | 0.0005 | 0.0001 | 0.0004 | 0 | 0 | 0 | 0 | 0.0001 |
Bud Neck | 0.0008 | 0.0003 | 0.0035 | 0.0006 | 0.0014 | 0.0006 | 0.0004 | 0.0006 | 0.0001 | 0.0001 | 0.0042 | 0.0011 | 0.0026 | 0.0001 | 0.0002 | 0.0002 | 0.0033 | 0.0008 |
Bud Periphery | 0.0025 | 0 | 0.0005 | 0.0001 | 0.0006 | 0.0001 | 0.0011 | 0.0002 | 0 | 0 | 0.0004 | 0.0003 | 0.0005 | 0 | 0 | 0.0001 | 0.0001 | 0.0002 |
Bud Site | 0.0062 | 0.001 | 0.005 | 0.0005 | 0.0016 | 0 | 0.0006 | 0.0019 | 0.0007 | 0.0001 | 0.0023 | 0.0001 | 0.0052 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0001 |
Cell Periphery | 0.0005 | 0.0001 | 0.0002 | 0 | 0.0005 | 0 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0 | 0.0021 | 0 | 0 | 0.0001 | 0.0001 | 0.0001 |
Cytoplasm | 0.0128 | 0.0288 | 0.0365 | 0.0085 | 0.0203 | 0.0126 | 0.0156 | 0.013 | 0.0289 | 0.0467 | 0.0405 | 0.0072 | 0.0086 | 0.0283 | 0.0555 | 0.0139 | 0.043 | 0.0158 |
Cytoplasmic Foci | 0.0085 | 0.0057 | 0.0162 | 0.0273 | 0.0459 | 0.0023 | 0.0046 | 0.0037 | 0.0127 | 0.0005 | 0.0225 | 0.0023 | 0.0892 | 0.0002 | 0.0003 | 0.0038 | 0.0002 | 0.0003 |
Eisosomes | 0.0002 | 0 | 0.0003 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0.0002 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0168 | 0.0099 | 0.0128 | 0.0083 | 0.0509 | 0.0042 | 0.0201 | 0.0248 | 0.0174 | 0.0385 | 0.0152 | 0.0114 | 0.0023 | 0.0315 | 0.0462 | 0.0254 | 0.0207 | 0.0478 |
Endosome | 0.0758 | 0.0135 | 0.0331 | 0.0176 | 0.0807 | 0.0045 | 0.0283 | 0.0129 | 0.0112 | 0.0033 | 0.071 | 0.0123 | 0.14 | 0.0052 | 0.0052 | 0.0446 | 0.0078 | 0.0078 |
Golgi | 0.0073 | 0.0006 | 0.0173 | 0.0118 | 0.0182 | 0.0049 | 0.0048 | 0.0007 | 0.0026 | 0.0001 | 0.041 | 0.0067 | 0.0161 | 0 | 0.0001 | 0.0009 | 0.0001 | 0.0004 |
Lipid Particles | 0.0069 | 0.0034 | 0.0102 | 0.0037 | 0.0274 | 0.0033 | 0.0354 | 0.0006 | 0.0014 | 0.0001 | 0.0029 | 0.0038 | 0.2372 | 0.0001 | 0.0001 | 0.0157 | 0.0001 | 0.0006 |
Mitochondria | 0.0086 | 0.0005 | 0.0554 | 0.0016 | 0.0038 | 0.0012 | 0.0043 | 0.0007 | 0.0014 | 0.0005 | 0.0062 | 0.0029 | 0.0028 | 0.0004 | 0.0007 | 0.001 | 0.0008 | 0.0063 |
None | 0.011 | 0.0005 | 0.0171 | 0.0003 | 0.0044 | 0.0006 | 0.0057 | 0.0003 | 0.0013 | 0.0056 | 0.0375 | 0.01 | 0.0144 | 0.0003 | 0.0023 | 0.0039 | 0.0011 | 0.001 |
Nuclear Periphery | 0.5237 | 0.5372 | 0.4411 | 0.4687 | 0.3979 | 0.4647 | 0.6482 | 0.683 | 0.7293 | 0.8007 | 0.5273 | 0.6472 | 0.0354 | 0.7417 | 0.644 | 0.681 | 0.5297 | 0.5331 |
Nucleolus | 0.0084 | 0.0016 | 0.0005 | 0.0003 | 0.0022 | 0.0006 | 0.0017 | 0.0006 | 0.0002 | 0.0001 | 0.0026 | 0.0022 | 0.1482 | 0.0002 | 0.0003 | 0.0006 | 0.0011 | 0.0011 |
Nucleus | 0.2392 | 0.3853 | 0.2897 | 0.4356 | 0.2394 | 0.4885 | 0.1653 | 0.2385 | 0.1609 | 0.0904 | 0.1876 | 0.28 | 0.0239 | 0.1751 | 0.2306 | 0.1829 | 0.3671 | 0.3679 |
Peroxisomes | 0.0018 | 0.0002 | 0.0085 | 0.0042 | 0.013 | 0.0025 | 0.0035 | 0.0005 | 0.0155 | 0 | 0.0037 | 0.0004 | 0.0188 | 0 | 0 | 0.0003 | 0 | 0 |
Punctate Nuclear | 0.0123 | 0.0027 | 0.0034 | 0.0017 | 0.0267 | 0.0014 | 0.0062 | 0.0039 | 0.0019 | 0.0013 | 0.0204 | 0.0026 | 0.1939 | 0.0028 | 0.0041 | 0.0104 | 0.0023 | 0.0041 |
Vacuole | 0.0329 | 0.0045 | 0.0058 | 0.0007 | 0.0215 | 0.0032 | 0.0081 | 0.0062 | 0.0017 | 0.0013 | 0.0051 | 0.0024 | 0.0482 | 0.0061 | 0.0021 | 0.0064 | 0.0154 | 0.0034 |
Vacuole Periphery | 0.0118 | 0.0037 | 0.0079 | 0.0044 | 0.0102 | 0.0039 | 0.0104 | 0.0074 | 0.0087 | 0.0095 | 0.0067 | 0.0051 | 0.0057 | 0.0071 | 0.0067 | 0.0064 | 0.0065 | 0.0076 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 54.8983 | 37.6804 | 29.3134 | 36.6016 | 35.4788 | 26.1599 | 32.7986 | 25.403 | 24.5718 | 41.8372 |
Translational Efficiency | 0.5809 | 0.6476 | 0.7325 | 0.5455 | 0.608 | 0.929 | 0.5271 | 0.5808 | 0.5693 | 0.5259 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1872 | 1462 | 171 | 265 | 1700 | 2060 | 1976 | 1533 | 3572 | 3522 | 2147 | 1798 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 997.66 | 975.49 | 1218.59 | 1268.68 | 787.33 | 962.44 | 1135.77 | 1141.14 | 897.56 | 967.86 | 1142.37 | 1159.94 |
Standard Deviation | 120.62 | 167.23 | 163.07 | 159.02 | 113.06 | 135.36 | 159.37 | 183.65 | 157.30 | 149.56 | 161.23 | 185.81 |
Intensity Change Log 2 | — | -0.032421 | 0.288593 | 0.346708 | — | 0.289728 | 0.528630 | 0.535435 | — | 0.118600 | 0.399419 | 0.433009 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000355 | 0.000308 | 0.001622 | 0.000571 | 0.000156 | 0.000183 | 0.000390 | 0.000430 | 0.000260 | 0.000235 | 0.000488 | 0.000451 |
Bud Neck | 0.006971 | 0.001373 | 0.007413 | 0.008735 | 0.000536 | 0.001205 | 0.004590 | 0.004935 | 0.003909 | 0.001275 | 0.004815 | 0.005495 |
Bud Site | 0.002031 | 0.000602 | 0.000724 | 0.002094 | 0.000230 | 0.000438 | 0.000283 | 0.001998 | 0.001174 | 0.000506 | 0.000318 | 0.002012 |
Cell Periphery | 0.000054 | 0.000059 | 0.000094 | 0.000085 | 0.000072 | 0.000038 | 0.000025 | 0.000056 | 0.000063 | 0.000047 | 0.000031 | 0.000060 |
Cytoplasm | 0.078143 | 0.034759 | 0.010655 | 0.061131 | 0.047110 | 0.023162 | 0.012566 | 0.043828 | 0.063374 | 0.027976 | 0.012414 | 0.046378 |
Cytoplasmic Foci | 0.001593 | 0.003854 | 0.000728 | 0.001777 | 0.004424 | 0.003333 | 0.000113 | 0.000612 | 0.002941 | 0.003549 | 0.000162 | 0.000784 |
Eisosomes | 0.000012 | 0.000010 | 0.000017 | 0.000013 | 0.000014 | 0.000009 | 0.000004 | 0.000005 | 0.000013 | 0.000009 | 0.000005 | 0.000006 |
Endoplasmic Reticulum | 0.023913 | 0.021592 | 0.044573 | 0.020835 | 0.026691 | 0.018753 | 0.013288 | 0.015416 | 0.025235 | 0.019931 | 0.015780 | 0.016215 |
Endosome | 0.002783 | 0.005331 | 0.002711 | 0.007209 | 0.002545 | 0.002693 | 0.000941 | 0.002511 | 0.002669 | 0.003788 | 0.001082 | 0.003204 |
Golgi | 0.000827 | 0.002375 | 0.000172 | 0.000193 | 0.000270 | 0.001182 | 0.000077 | 0.000192 | 0.000562 | 0.001677 | 0.000085 | 0.000192 |
Lipid Particles | 0.000971 | 0.002569 | 0.001469 | 0.003035 | 0.014759 | 0.004428 | 0.000195 | 0.000600 | 0.007533 | 0.003656 | 0.000296 | 0.000959 |
Mitochondria | 0.004403 | 0.005225 | 0.003278 | 0.001501 | 0.005363 | 0.006921 | 0.003430 | 0.003079 | 0.004860 | 0.006217 | 0.003418 | 0.002847 |
Mitotic Spindle | 0.020704 | 0.016152 | 0.010593 | 0.268373* | 0.002033 | 0.010087 | 0.010019 | 0.064991 | 0.011818 | 0.012604 | 0.010065 | 0.094967 |
None | 0.002884 | 0.003970 | 0.000996 | 0.002845 | 0.010361 | 0.002938 | 0.000951 | 0.002960 | 0.006442 | 0.003366 | 0.000955 | 0.002943 |
Nuclear Periphery | 0.300597* | 0.468081* | 0.335724* | 0.241160* | 0.415986* | 0.542641* | 0.571326* | 0.453805* | 0.355513* | 0.511691* | 0.552561* | 0.422464* |
Nuclear Periphery Foci | 0.036075 | 0.271348* | 0.181046 | 0.189400 | 0.306776* | 0.190279* | 0.106442 | 0.224903* | 0.164908 | 0.223931* | 0.112384 | 0.219670* |
Nucleolus | 0.010672 | 0.016118 | 0.034088 | 0.006833 | 0.023530 | 0.019771 | 0.027931 | 0.010655 | 0.016792 | 0.018254 | 0.028421 | 0.010092 |
Nucleus | 0.487750* | 0.134703* | 0.345459* | 0.120044 | 0.135032* | 0.162150* | 0.242393* | 0.157440* | 0.319883* | 0.150756* | 0.250602* | 0.151928* |
Peroxisomes | 0.000250 | 0.000385 | 0.000545 | 0.000228 | 0.000404 | 0.000795 | 0.000101 | 0.000110 | 0.000323 | 0.000625 | 0.000136 | 0.000128 |
Vacuole | 0.018338 | 0.007876 | 0.016424 | 0.063313 | 0.002581 | 0.006449 | 0.003950 | 0.010105 | 0.010839 | 0.007041 | 0.004943 | 0.017947 |
Vacuole Periphery | 0.000674 | 0.003310 | 0.001670 | 0.000623 | 0.001125 | 0.002547 | 0.000984 | 0.001368 | 0.000889 | 0.002863 | 0.001038 | 0.001258 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 2.04 | -7.18 | -4.43 | -5.11 | 3.93 | -0.95 | -11.37 | -9.38 | -7.80 | -2.69 | 1.74 | -8.82 | -7.86 | -8.58 | -1.46 |
Bud Neck | 10.25 | 0.14 | -4.49 | -8.14 | -4.31 | -2.61 | -13.34 | -10.93 | -9.65 | -3.61 | 8.74 | -2.10 | -6.65 | -12.30 | -5.37 |
Bud Site | 3.85 | 2.82 | -3.16 | -4.01 | -3.81 | -1.32 | -1.56 | -3.48 | -3.13 | -3.28 | 3.28 | 3.85 | -3.24 | -4.58 | -4.74 |
Cell Periphery | -0.49 | -4.14 | -7.24 | -5.95 | -1.81 | 5.78 | 8.05 | 0.43 | -5.01 | -7.05 | 3.61 | 8.06 | -2.68 | -5.31 | -8.33 |
Cytoplasm | 11.89 | 17.23 | 1.65 | -5.22 | -8.26 | 6.85 | 10.50 | -1.23 | -8.58 | -12.53 | 14.48 | 22.14 | 3.61 | -8.76 | -14.71 |
Cytoplasmic Foci | -3.10 | 2.70 | -3.13 | 1.49 | -6.22 | 1.61 | 7.17 | 6.03 | 5.10 | -7.24 | -1.03 | 8.61 | 5.65 | 5.87 | -9.75 |
Eisosomes | 5.27 | -1.66 | -6.82 | -8.83 | -4.17 | 4.52 | 10.50 | 5.63 | 1.05 | -5.57 | 7.06 | 13.65 | 2.96 | -3.28 | -8.39 |
Endoplasmic Reticulum | 1.09 | -5.23 | 2.61 | 1.76 | 6.03 | 5.61 | 10.11 | 8.02 | 2.61 | -2.23 | 5.05 | 9.25 | 9.05 | 3.99 | 0.16 |
Endosome | -2.65 | -0.08 | -7.80 | -3.05 | -7.86 | -0.43 | 4.52 | -1.59 | -1.26 | -9.83 | -2.39 | 6.76 | -4.17 | -0.39 | -12.83 |
Golgi | -2.27 | 1.25 | 0.96 | 2.82 | -2.53 | -2.82 | 5.34 | 1.24 | 3.00 | -2.98 | -3.20 | 2.21 | 1.30 | 4.03 | -3.44 |
Lipid Particles | -5.38 | -2.30 | -6.60 | -2.90 | -4.76 | 9.51 | 14.43 | 13.81 | 9.04 | -8.61 | 6.95 | 14.53 | 12.42 | 8.73 | -10.14 |
Mitochondria | -1.02 | 3.00 | 6.05 | 4.64 | 3.48 | -1.80 | 4.43 | 5.38 | 4.11 | 1.23 | -2.23 | 4.05 | 5.90 | 5.15 | 2.00 |
Mitotic Spindle | 2.29 | 1.28 | -9.68 | -10.14 | -9.77 | -5.36 | -7.00 | -15.18 | -13.21 | -12.68 | 0.56 | 0.33 | -15.59 | -15.94 | -15.75 |
None | -1.10 | 8.57 | -2.24 | -1.71 | -4.08 | 7.33 | 9.51 | 6.65 | -0.79 | -5.00 | 6.12 | 11.49 | 4.65 | -0.85 | -6.27 |
Nuclear Periphery | -19.69* | -2.74 | 8.43 | 22.19* | 7.33 | -11.60 | -11.61 | 1.57 | 12.77 | 12.78 | -23.28 | -21.57 | -3.20 | 15.87 | 15.60 |
Nuclear Periphery Foci | -31.21* | -8.90 | -6.89 | 13.66 | 3.75 | 12.80 | 25.23* | 10.82 | -1.70 | -14.66 | -9.77 | 10.04 | -3.75 | 4.94 | -12.70 |
Nucleolus | -3.43 | -4.32 | 0.53 | 2.49 | 4.27 | 1.76 | -2.17 | 5.61 | 4.46 | 8.62 | -0.96 | -6.88 | 3.76 | 4.72 | 9.62 |
Nucleus | 38.93* | 5.88 | 17.27 | -4.36 | 5.24 | -3.67 | -14.88 | -6.34 | -3.00 | 7.74 | 25.66 | 6.71 | 16.46 | -5.38 | 8.72 |
Peroxisomes | -1.24 | -3.78 | -1.61 | 0.28 | 2.23 | -1.25 | 6.08 | 4.68 | 2.06 | -1.87 | -1.61 | 5.63 | 3.69 | 2.33 | -1.69 |
Vacuole | 9.51 | 2.29 | -9.92 | -11.39 | -10.34 | -4.48 | -3.80 | -9.57 | -6.13 | -7.44 | 7.54 | 8.38 | -7.92 | -11.40 | -11.72 |
Vacuole Periphery | -5.50 | -6.56 | -0.08 | 5.45 | 6.23 | -4.10 | 0.84 | -0.77 | 3.26 | -1.27 | -7.03 | -1.51 | -2.04 | 5.13 | -0.69 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
---|
Gene | Images |
---|