Standard name
Human Ortholog
Description Protein kinase; along with its paralog, ALK2, required for proper spindle positioning and nuclear segregation following mitotic arrest, proper organization of cell polarity factors in mitosis, proper localization of formins and polarity factors, and survival in cells that activate spindle assembly checkpoint; phosphorylated in response to DNA damage; ALK1 has a paralog, ALK2, that arose from the whole genome duplication; similar to mammalian haspins
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0.09 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0.05 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 – – – – – –
Cell Periphery 0.05 0.05 0.08 0 0 0 0 0 0 0 0 0 0 0 0 – 0.07 0.11 0 0 0.09 0 0 0
Cytoplasm 0.97 0.96 0.96 0.92 0.94 0.83 0.8 0.71 0.61 0.54 0.56 0.51 0.98 0.98 0.99 – 0.93 0.95 0.74 0.75 0.56 0.81 0.86 0.84
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0.08 0 0 0 0.05 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0.09 0.06 0.41 0.35 0.45 0.55 0.69 0.62 0.61 0 0 0 – 0 0 0.05 0 0.09 0.05 0 0.05
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 2 2 0 0 0 0 2 0 0 0 0 0 0 0 – 1 2 6 11 24 2 1 4
Bud 0 4 4 2 2 4 2 11 12 12 16 13 0 0 0 – 1 0 4 10 3 1 3 4
Bud Neck 1 0 1 0 2 0 0 1 1 1 0 0 1 1 1 – 1 0 5 13 14 1 0 0
Bud Site 0 0 0 0 0 1 0 0 1 1 3 1 0 0 0 – 0 0 – – – – – –
Cell Periphery 8 16 21 6 8 4 6 7 7 6 9 13 0 0 5 – 17 30 6 11 25 1 0 0
Cytoplasm 152 315 263 174 265 353 284 361 210 278 235 222 121 121 188 – 225 249 130 251 156 77 176 192
Endoplasmic Reticulum 3 3 1 1 0 1 0 1 0 3 0 2 1 1 0 – 19 10 1 2 13 1 2 0
Endosome 0 1 3 0 5 2 5 5 2 3 2 3 0 0 0 – 3 2 0 5 3 0 1 2
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 3 1 0 0 0 3 0
Mitochondria 2 12 11 17 17 173 124 231 190 359 259 265 0 0 0 – 8 8 9 11 24 5 6 10
Nucleus 1 1 0 4 0 0 0 4 0 3 1 2 2 2 1 – 1 1 0 1 0 0 0 0
Nuclear Periphery 0 0 0 4 1 0 0 0 3 4 0 1 0 0 0 – 0 0 0 0 1 0 0 0
Nucleolus 0 0 0 2 0 2 0 2 1 1 0 1 0 0 0 – 0 1 0 0 0 0 0 0
Peroxisomes 0 0 0 0 2 2 1 1 0 0 0 0 0 0 0 – 0 1 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 3 4 9 7 9 12 12 0 0 0 – 1 0 1 1 0 0 2 3
Vac/Vac Membrane 1 0 3 3 6 6 8 7 4 11 8 8 0 0 0 – 5 0 3 6 4 1 3 4
Unique Cell Count 156 329 275 190 282 423 353 510 344 519 418 433 123 123 189 242 263 176 333 279 95 204 229
Labelled Cell Count 168 354 309 213 308 551 434 642 438 691 545 543 125 125 195 282 307 176 333 279 95 204 229


Ambiguous

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.8 4.6 4.5 4.2 4.3 3.3 3.5 3.6 3.1 2.9 2.9 3.2 5.2 5.2 5.2 4.8 5.0 6.2 4.5 5.3 5.5
Std Deviation (1e-4) 1.7 1.4 1.7 2.1 2.1 1.5 1.9 1.7 1.0 0.9 0.9 1.5 1.0 1.0 1.1 1.5 1.5 2.2 1.1 1.9 1.9
Intensity Change (Log2) – – – -0.09 -0.06 -0.43 -0.34 -0.31 -0.53 -0.62 -0.64 -0.49 0.22 0.22 0.22 0.12 0.17 0.47 0.02 0.26 0.3


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.7715 0.4946 0.9188 1.2918 2.0554 1.065 1.9236 1.4808 1.9391 2.7228 2.6487 2.6616 1.1013 2.2777 2.1569 2.8336 3.2742 2.2724
Actin 0.0581 0.0039 0.0089 0.0005 0.0272 0.016 0.1581 0.001 0.0061 0.0005 0.0289 0.0112 0.0004 0.0003 0.001 0.0025 0.002 0.0095
Bud 0.0023 0.0118 0.0021 0.0002 0.0143 0.0039 0.0025 0.0002 0.0031 0.0001 0.0129 0.0005 0.0001 0.0001 0.0001 0.0029 0.0001 0.0007
Bud Neck 0.0039 0.0002 0.0006 0.0015 0.0018 0.0078 0.0025 0.001 0.0013 0.0005 0.0032 0.0015 0.0006 0.0002 0.0005 0.0025 0.0019 0.0029
Bud Periphery 0.0079 0.0051 0.0059 0.001 0.024 0.009 0.0041 0.0007 0.011 0.0003 0.0323 0.0021 0.0007 0.0002 0.0003 0.0051 0.0008 0.0016
Bud Site 0.0131 0.0203 0.008 0.0007 0.0057 0.0014 0.0095 0.0086 0.0045 0.0002 0.0025 0.0003 0.0009 0.0022 0.0007 0.0095 0.0005 0.001
Cell Periphery 0.0012 0.0015 0.0016 0.0048 0.0038 0.001 0.0005 0.001 0.0012 0.0023 0.004 0.0012 0.0035 0.0036 0.0032 0.0042 0.0143 0.0017
Cytoplasm 0.122 0.2632 0.2854 0.6301 0.5795 0.4073 0.1148 0.1966 0.2535 0.6674 0.6918 0.3974 0.2368 0.2361 0.4227 0.771 0.881 0.4592
Cytoplasmic Foci 0.016 0.0036 0.0159 0.0034 0.0177 0.0101 0.0236 0.0025 0.0086 0.0019 0.0154 0.0046 0.002 0.0017 0.002 0.0029 0.0023 0.002
Eisosomes 0.0006 0.0002 0.0003 0.0001 0.0003 0.0002 0.001 0.0001 0.0003 0.0001 0.0002 0.0001 0.0002 0.0003 0.0003 0.0001 0.0007 0.0001
Endoplasmic Reticulum 0.0045 0.0016 0.0031 0.0026 0.004 0.0022 0.0023 0.0008 0.0034 0.0032 0.0054 0.0013 0.0016 0.0012 0.0026 0.0031 0.0024 0.0015
Endosome 0.0194 0.0009 0.0116 0.0008 0.0536 0.0065 0.02 0.0005 0.0012 0.0004 0.0026 0.0071 0.0003 0.0002 0.0003 0.0005 0.0008 0.0002
Golgi 0.0109 0.0008 0.006 0.0002 0.0263 0.0019 0.0228 0.0001 0.0008 0.0001 0.0061 0.0032 0.0001 0.0001 0.0001 0.0003 0.0003 0.0001
Lipid Particles 0.0106 0.0002 0.0066 0.0002 0.0171 0.0026 0.018 0.0001 0.0025 0.0002 0.0159 0.0004 0.0003 0.0001 0.0002 0.0006 0.0002 0.0001
Mitochondria 0.0119 0.0002 0.0092 0.0001 0.0486 0.0016 0.0122 0.0001 0.0005 0.0001 0.0008 0.0009 0.0001 0.0001 0.0001 0.0003 0.0001 0.0001
None 0.6912 0.6833 0.6224 0.3519 0.1254 0.5118 0.5649 0.7854 0.6964 0.3211 0.1333 0.5616 0.7512 0.7522 0.5644 0.1897 0.0916 0.518
Nuclear Periphery 0.0113 0.0003 0.0009 0.0002 0.0074 0.0045 0.0011 0.0002 0.0003 0.0002 0.001 0.0002 0.0002 0.0001 0.0002 0.0005 0.0002 0.0002
Nucleolus 0.0006 0.0002 0.0003 0.0001 0.0034 0.0003 0.0002 0.0001 0.0002 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0.0004 0 0
Nucleus 0.0025 0.0011 0.0007 0.0004 0.0126 0.003 0.0004 0.0004 0.0004 0.0004 0.0014 0.0003 0.0003 0.0004 0.0004 0.0015 0.0002 0.0003
Peroxisomes 0.005 0.0001 0.0057 0 0.0039 0.0043 0.0385 0.0001 0.0035 0 0.0394 0.0051 0 0 0.0001 0.0001 0.0001 0.0001
Punctate Nuclear 0.0034 0.0007 0.0014 0.0001 0.0134 0.0034 0.0014 0.0002 0.0005 0.0001 0.0009 0.0004 0.0002 0.0002 0.0002 0.0007 0.0001 0.0002
Vacuole 0.0023 0.0009 0.0026 0.001 0.0071 0.0008 0.0011 0.0005 0.0007 0.0008 0.0018 0.0003 0.0004 0.0005 0.0004 0.0016 0.0004 0.0002
Vacuole Periphery 0.0012 0.0001 0.0007 0 0.0031 0.0003 0.0005 0 0.0001 0 0.0002 0.0001 0 0 0 0.0001 0 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 7.2497 38.0206 28.5336 25.6838 11.2795 4.3474 53.7323 52.3346 30.3194 14.7748
Translational Efficiency 0.7772 1.1132 1.2079 0.647 0.5397 1.0179 0.9161 0.7699 0.8996 0.7604

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
163 1452 1082 1316 1509 1085 1871 1555 1672 2537 2953 2871

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 862.15 758.52 834.09 1049.38 695.24 717.56 861.13 1071.67 711.51 741.00 851.22 1061.45
Standard Deviation 119.47 113.75 101.91 163.57 83.19 92.01 114.01 158.72 100.44 106.94 110.50 161.35
Intensity Change Log 2 — -0.184752 -0.047736 0.283526 — 0.045588 0.308720 0.624278 — -0.077359 0.122342 0.445648

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.001203 0.000368 0.000379 0.039843 0.000428 0.001063 0.001331 0.026194 0.000503 0.000665 0.000982 0.032450
Bud Neck 0.007681 0.021772 0.003702 0.022384 0.022982 0.044854 0.007918 0.020617 0.021490 0.031644 0.006373 0.021427
Bud Site 0.043362 0.017255 0.011686 0.188043* 0.007805 0.015540 0.033543 0.144141* 0.011271 0.016521 0.025534 0.164265*
Cell Periphery 0.000487 0.000306 0.000343 0.013679 0.000328 0.001015 0.000709 0.008633 0.000344 0.000610 0.000575 0.010946
Cytoplasm 0.270090* 0.540998* 0.526517* 0.399605* 0.469682* 0.417317* 0.593670* 0.450077* 0.450224* 0.488103* 0.569065* 0.426942*
Cytoplasmic Foci 0.099396 0.169016 0.034655 0.039622 0.218004* 0.260416* 0.029402 0.039224 0.206441* 0.208105* 0.031327 0.039406
Eisosomes 0.000192 0.000286 0.000074 0.003588 0.000695 0.000709 0.000197 0.002511 0.000646 0.000467 0.000152 0.003005
Endoplasmic Reticulum 0.001787 0.000962 0.002199 0.005967 0.001336 0.000442 0.002579 0.005956 0.001380 0.000739 0.002440 0.005961
Endosome 0.034099 0.013286 0.002695 0.002271 0.010449 0.022618 0.001495 0.004538 0.012754 0.017277 0.001935 0.003499
Golgi 0.023099 0.003166 0.000111 0.003252 0.003340 0.006658 0.000179 0.003859 0.005266 0.004660 0.000154 0.003581
Lipid Particles 0.005624 0.004275 0.001617 0.010371 0.011451 0.009831 0.001027 0.004154 0.010883 0.006651 0.001243 0.007004
Mitochondria 0.000754 0.002878 0.000318 0.001643 0.005039 0.013105 0.000459 0.004008 0.004622 0.007252 0.000407 0.002924
Mitotic Spindle 0.008317 0.004613 0.002326 0.012985 0.000726 0.005176 0.001822 0.010237 0.001466 0.004854 0.002007 0.011497
None 0.000342 0.005335 0.003982 0.001072 0.002263 0.003596 0.002389 0.002709 0.002076 0.004591 0.002973 0.001959
Nuclear Periphery 0.000520 0.000533 0.000722 0.000210 0.000414 0.000263 0.000352 0.000245 0.000424 0.000418 0.000488 0.000229
Nuclear Periphery Foci 0.001349 0.000812 0.001869 0.003224 0.000397 0.000229 0.001712 0.003545 0.000490 0.000562 0.001770 0.003398
Nucleolus 0.000262 0.001134 0.000447 0.000115 0.001002 0.001231 0.000121 0.000106 0.000930 0.001175 0.000240 0.000110
Nucleus 0.104082 0.136724* 0.337079* 0.074094 0.173669* 0.108134 0.217293* 0.081787 0.166885* 0.124497 0.261184* 0.078261
Peroxisomes 0.009742 0.006722 0.000341 0.003415 0.005996 0.017974 0.000408 0.002765 0.006362 0.011534 0.000383 0.003063
Vacuole 0.379891* 0.068378 0.068775 0.172824 0.063125 0.068001 0.103160 0.182667 0.094006 0.068217 0.090561 0.178156
Vacuole Periphery 0.007719 0.001179 0.000162 0.001791 0.000870 0.001831 0.000233 0.002027 0.001538 0.001458 0.000207 0.001919

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 1.83 1.69 -13.68 -14.05 -14.03 -1.46 -6.60 -11.87 -10.85 -11.18 -1.00 -4.80 -18.23 -17.86 -17.79
Bud Neck -6.80 0.75 -7.18 0.65 -13.70 -6.42 9.89 3.33 8.51 -7.55 -4.93 10.95 1.78 7.24 -13.88
Bud Site 1.40 2.10 -11.07 -21.92 -22.71* -4.05 -8.02 -20.83 -18.27 -14.40 -3.88 -5.84 -29.81 -28.23 -25.40
Cell Periphery 0.29 0.08 -9.45 -9.63 -9.59 -1.10 -6.15 -9.63 -5.03 -8.96 -1.03 -4.82 -13.29 -11.05 -12.87
Cytoplasm -12.12 -9.77 -2.78 20.25 14.03 5.11 -8.31 9.62 3.46 17.57 -4.44 -9.31 11.45 17.20 23.20
Cytoplasmic Foci -9.72 5.33 3.60 22.35 -4.02 -5.03 35.84 29.38 29.94 -7.40 -0.58 35.23 30.12 36.26 -8.44
Eisosomes -1.98 1.80 -8.77 -8.32 -9.30 -0.04 6.03 -3.84 -3.15 -6.99 1.72 6.54 -7.23 -8.39 -11.49
Endoplasmic Reticulum 2.68 -3.06 -10.68 -13.15 -8.08 5.23 -7.56 -7.09 -8.92 -3.67 3.65 -7.83 -11.84 -14.23 -7.36
Endosome 1.82 4.16 4.29 9.04 1.93 -6.79 12.33 7.90 11.18 -4.31 -4.45 12.12 10.26 13.97 -3.42
Golgi 1.68 2.19 1.90 1.52 -2.01 -3.61 10.88 1.87 4.23 -2.57 -0.40 6.52 2.97 4.01 -3.23
Lipid Particles -0.22 1.45 -3.01 -4.31 -6.57 1.26 12.50 7.07 4.34 -9.99 4.53 12.28 3.76 -0.66 -9.81
Mitochondria -4.38 0.86 -1.47 3.09 -2.17 -4.07 6.57 3.15 5.67 -2.59 -2.46 6.65 4.11 6.12 -3.20
Mitotic Spindle 0.29 1.09 -3.65 -5.36 -6.41 -2.95 -2.27 -6.65 -3.82 -5.98 -3.58 -1.34 -9.38 -6.56 -8.83
None -5.15 -6.50 -4.24 4.31 4.81 -1.14 0.18 0.26 1.29 0.18 -2.95 -1.16 0.74 3.80 2.69
Nuclear Periphery 0.41 -3.40 -0.54 -0.61 3.50 2.52 -1.86 -4.22 -8.48 -3.70 0.16 -4.14 -4.30 -2.09 -0.98
Nuclear Periphery Foci 1.63 -2.77 -7.73 -8.37 -6.47 2.18 -9.67 -11.68 -12.22 -8.93 -0.39 -10.28 -14.26 -13.81 -10.81
Nucleolus -5.39 -3.00 -1.06 4.92 1.95 -0.58 5.77 3.74 2.80 -4.44 -1.11 4.96 4.16 5.19 -2.10
Nucleus -1.61 -13.11 1.00 8.09 27.69 10.35 -10.67 12.02 0.32 22.07 8.20 -18.70 13.75 6.06 34.80
Peroxisomes -0.34 2.03 1.11 4.36 -6.29 -7.28 11.05 6.02 9.83 -6.50 -5.61 10.64 5.87 10.45 -9.04
Vacuole 14.42 13.58 6.50 -25.76 -21.47 -1.63 -15.51 -31.13 -28.76 -18.80 6.48 -3.44 -27.13 -38.78 -27.37
Vacuole Periphery 1.45 1.90 1.53 0.50 -1.81 -3.37 5.00 -0.28 1.63 -1.85 -0.79 4.22 0.65 1.40 -2.56
Standard name
Human Ortholog
Description Protein kinase; along with its paralog, ALK2, required for proper spindle positioning and nuclear segregation following mitotic arrest, proper organization of cell polarity factors in mitosis, proper localization of formins and polarity factors, and survival in cells that activate spindle assembly checkpoint; phosphorylated in response to DNA damage; ALK1 has a paralog, ALK2, that arose from the whole genome duplication; similar to mammalian haspins
Localization
Cell Percentages cytoplasm (35%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-10

Alk1

Alk1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Alk1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available