Standard name | |
---|---|
Human Ortholog | |
Description | Subunit of the oligosaccharyltransferase complex of the ER lumen; complex catalyzes asparagine-linked glycosylation of newly synthesized proteins; forms a subcomplex with Ost3p and Ost4p and is directly involved in catalysis |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 5 | 31 | 29 | 25 | 41 | 52 | 39 | 76 | 68 | 81 | 67 | 95 | 2 | 3 | 3 | 23 | 14 | 6 | 4 | 5 | 12 | 5 | 8 | 7 |
Bud | 0 | 6 | 1 | 0 | 1 | 3 | 8 | 12 | 5 | 3 | 8 | 8 | 0 | 0 | 1 | 2 | 0 | 0 | 0 | 3 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 2 | 10 | 11 | 15 | 20 | 25 | 26 | 31 | 44 | 59 | 47 | 76 | 0 | 12 | 17 | 44 | 18 | 18 | 0 | 2 | 2 | 2 | 4 | 6 |
Cytoplasm | 8 | 28 | 16 | 14 | 21 | 28 | 38 | 28 | 38 | 45 | 40 | 36 | 5 | 10 | 11 | 13 | 41 | 16 | 2 | 6 | 0 | 1 | 0 | 2 |
Endoplasmic Reticulum | 173 | 291 | 264 | 147 | 231 | 321 | 239 | 534 | 358 | 369 | 283 | 262 | 61 | 68 | 121 | 6 | 4 | 0 | 191 | 320 | 274 | 91 | 171 | 161 |
Endosome | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 |
Golgi | 9 | 4 | 64 | 64 | 117 | 48 | 53 | 37 | 27 | 45 | 14 | 70 | 0 | 0 | 1 | 20 | 18 | 23 | 2 | 2 | 4 | 6 | 5 | 6 |
Mitochondria | 2 | 17 | 16 | 33 | 21 | 72 | 41 | 147 | 122 | 123 | 171 | 102 | 0 | 0 | 0 | 15 | 17 | 7 | 0 | 3 | 3 | 7 | 4 | 3 |
Nucleus | 0 | 1 | 1 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 2 | 9 | 3 | 4 | 6 | 5 | 31 | 23 | 21 | 25 | 11 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 13 | 13 | 5 | 4 | 38 | 6 | 44 | 18 | 8 | 46 | 22 | 56 | 0 | 2 | 0 | 14 | 7 | 1 | 1 | 7 | 1 | 3 | 3 | 3 |
Unique Cell Count | 189 | 340 | 308 | 199 | 333 | 397 | 361 | 683 | 487 | 575 | 481 | 472 | 65 | 86 | 140 | 109 | 104 | 56 | 204 | 358 | 304 | 123 | 204 | 197 |
Labelled Cell Count | 212 | 404 | 416 | 305 | 495 | 561 | 494 | 917 | 693 | 792 | 677 | 717 | 69 | 96 | 155 | 137 | 119 | 71 | 204 | 358 | 304 | 123 | 204 | 197 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 15.4 | 15.1 | 16.5 | 17.7 | 18.5 | 16.5 | 16.1 | 15.1 | 15.5 | 14.8 | 13.7 | 15.6 | 15.5 | 14.9 | 16.7 | 13.2 | 14.4 | 18.3 | 17.1 | 18.3 | 18.7 |
Std Deviation (1e-4) | 2.2 | 2.7 | 2.9 | 2.9 | 3.1 | 2.6 | 2.8 | 2.8 | 2.8 | 3.2 | 3.1 | 3.0 | 2.3 | 2.1 | 2.5 | 2.2 | 3.1 | 4.3 | 3.0 | 3.0 | 3.0 |
Intensity Change (Log2) | – | – | – | 0.1 | 0.16 | -0.01 | -0.04 | -0.13 | -0.09 | -0.16 | -0.27 | -0.09 | -0.09 | -0.15 | 0.01 | -0.33 | -0.19 | 0.15 | 0.05 | 0.15 | 0.18 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 1.1 | 1.2 | 1.5 | 0.6 | 0.8 | 1.9 | 2.0 | 1.9 | 4.0 | -1.7 | -1.8 | -2.8 | 3.2 | 1.2 | 0.3 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 2.0 | 1.4 | 1.6 | 2.0 | 0.7 | 3.0 | 3.5 | 3.3 | 5.4 | 0 | 3.6 | 3.5 | 9.8 | 4.7 | 0 |
Cytoplasm | 0.9 | 0.6 | 1.0 | 2.5 | -0.8 | 1.4 | 1.5 | 1.7 | 1.3 | 0 | 2.1 | 1.1 | 2.4 | 8.7 | 0 |
Endoplasmic Reticulum | -3.3 | -4.9 | -1.7 | -5.8 | -2.8 | -4.1 | -6.8 | -8.0 | -8.8 | 1.8 | -1.5 | 0.2 | -15.1 | -15.1 | -13.2 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 2.9 | 4.0 | -3.1 | -2.1 | -7.4 | -6.6 | -5.6 | -8.2 | -2.2 | -4.0 | -4.6 | -5.6 | -0.5 | -0.8 | 3.3 |
Mitochondria | 4.2 | 0.6 | 5.2 | 2.8 | 6.4 | 7.2 | 6.3 | 9.8 | 6.3 | 0 | 0 | -2.7 | 2.9 | 3.6 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | -1.5 | -1.3 | -1.4 | 1.2 | 1.3 | 0.6 | 1.5 | -0.5 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 4.9 | 0 | 5.2 | 1.0 | 0 | 3.9 | 2.2 | 5.2 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 20.06 | 19.9775 | 20.3147 | 19.5636 | 18.4828 | 19.5021 | 17.851 | 21.9711 | 21.0606 | 19.002 | 19.5956 | 21.2496 | 19.6601 | 22.8785 | 22.8334 | 22.9391 | 21.5915 | 21.8868 |
Actin | 0.0075 | 0.0046 | 0.0004 | 0.0178 | 0.0062 | 0.0012 | 0.0627 | 0.0187 | 0.008 | 0.0266 | 0.0131 | 0.0003 | 0.047 | 0.0108 | 0.0006 | 0.0001 | 0.0164 | 0.0061 |
Bud | 0 | 0.0002 | 0 | 0.0003 | 0.0001 | 0 | 0.0007 | 0 | 0.0001 | 0.0003 | 0.0002 | 0 | 0.0006 | 0.0001 | 0 | 0 | 0.0001 | 0.0001 |
Bud Neck | 0.0003 | 0.0008 | 0.0005 | 0.0007 | 0.0014 | 0.0004 | 0.0023 | 0.0001 | 0.0006 | 0.0009 | 0.0056 | 0.0003 | 0.0004 | 0.0007 | 0.0003 | 0.0001 | 0.0008 | 0.0005 |
Bud Periphery | 0.0003 | 0.0004 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0.0018 | 0.0001 | 0.0002 | 0.0005 | 0.0003 | 0.0001 | 0.002 | 0.0003 | 0.0001 | 0 | 0.0003 | 0.0003 |
Bud Site | 0.0001 | 0.0023 | 0 | 0.0003 | 0.0002 | 0 | 0.0083 | 0 | 0.0015 | 0.0011 | 0.0043 | 0 | 0.0018 | 0.0008 | 0.0001 | 0 | 0.0002 | 0.0001 |
Cell Periphery | 0.0057 | 0.012 | 0.0047 | 0.0019 | 0.0023 | 0.0008 | 0.004 | 0.0036 | 0.0025 | 0.0019 | 0.0012 | 0.001 | 0.0052 | 0.0115 | 0.0051 | 0.003 | 0.0035 | 0.003 |
Cytoplasm | 0.0294 | 0.0507 | 0.0197 | 0.0094 | 0.0113 | 0.0068 | 0.0054 | 0.0027 | 0.002 | 0.0024 | 0.0132 | 0.0022 | 0.0065 | 0.0116 | 0.007 | 0.0008 | 0.0034 | 0.0026 |
Cytoplasmic Foci | 0.0005 | 0.0022 | 0.0004 | 0.005 | 0.0054 | 0.0005 | 0.0102 | 0.0002 | 0.0036 | 0.0175 | 0.0173 | 0.0003 | 0.007 | 0.0008 | 0.0014 | 0.0001 | 0.0022 | 0.0005 |
Eisosomes | 0.0002 | 0.0007 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0.0026 | 0.0006 | 0.0002 | 0.0006 | 0.0002 | 0.0001 | 0.0018 | 0.0007 | 0.0017 | 0.0005 | 0.0008 | 0.0002 |
Endoplasmic Reticulum | 0.8929 | 0.8483 | 0.9406 | 0.9283 | 0.9049 | 0.9488 | 0.7772 | 0.9355 | 0.9453 | 0.9102 | 0.8856 | 0.965 | 0.7919 | 0.9327 | 0.9477 | 0.974 | 0.8894 | 0.9479 |
Endosome | 0.0028 | 0.0074 | 0.003 | 0.0045 | 0.0208 | 0.0069 | 0.0278 | 0.002 | 0.0069 | 0.0123 | 0.0125 | 0.0048 | 0.0159 | 0.002 | 0.0033 | 0.0039 | 0.0228 | 0.0056 |
Golgi | 0.0025 | 0.0172 | 0.0032 | 0.0211 | 0.0097 | 0.0104 | 0.02 | 0.0051 | 0.0072 | 0.01 | 0.018 | 0.0048 | 0.0208 | 0.0078 | 0.0098 | 0.0085 | 0.0229 | 0.0132 |
Lipid Particles | 0.006 | 0.021 | 0.0124 | 0.0023 | 0.0104 | 0.0008 | 0.023 | 0.0039 | 0.0058 | 0.0031 | 0.0106 | 0.0021 | 0.009 | 0.0096 | 0.0014 | 0.0003 | 0.0124 | 0.0019 |
Mitochondria | 0.0003 | 0.0008 | 0.0003 | 0.0013 | 0.0012 | 0.001 | 0.0146 | 0.0011 | 0.0076 | 0.002 | 0.0006 | 0.0002 | 0.0274 | 0.0006 | 0.0008 | 0.0031 | 0.0122 | 0.0029 |
None | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0049 | 0.0001 | 0.0036 | 0.0001 | 0.0001 | 0.0006 | 0.0004 | 0.0001 | 0.005 | 0.0001 | 0.005 | 0 | 0.0005 | 0.0001 |
Nuclear Periphery | 0.0423 | 0.0217 | 0.0093 | 0.0048 | 0.0106 | 0.0188 | 0.0157 | 0.0206 | 0.005 | 0.0055 | 0.0036 | 0.0161 | 0.041 | 0.0072 | 0.0093 | 0.0036 | 0.0067 | 0.0109 |
Nucleolus | 0 | 0.0001 | 0 | 0 | 0.0004 | 0 | 0.0006 | 0 | 0 | 0.0001 | 0.0002 | 0 | 0.0003 | 0 | 0.0001 | 0 | 0.0001 | 0 |
Nucleus | 0.0017 | 0.002 | 0.0004 | 0.0002 | 0.0012 | 0.0008 | 0.0026 | 0.0024 | 0.0002 | 0.0004 | 0.0002 | 0.0002 | 0.0019 | 0.0003 | 0.0029 | 0.0001 | 0.0003 | 0.0002 |
Peroxisomes | 0 | 0.0001 | 0 | 0.0001 | 0.0007 | 0 | 0.0041 | 0 | 0.001 | 0.0012 | 0.0095 | 0 | 0.0029 | 0 | 0 | 0 | 0.0003 | 0.0001 |
Punctate Nuclear | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0028 | 0.0001 | 0.0024 | 0 | 0.0002 | 0.0011 | 0.0022 | 0 | 0.0032 | 0.0001 | 0.0009 | 0 | 0.0002 | 0 |
Vacuole | 0.0053 | 0.0063 | 0.0036 | 0.0011 | 0.004 | 0.0015 | 0.0075 | 0.0025 | 0.0015 | 0.0012 | 0.0008 | 0.0014 | 0.0045 | 0.0019 | 0.0015 | 0.0009 | 0.0026 | 0.0023 |
Vacuole Periphery | 0.002 | 0.001 | 0.0007 | 0.0003 | 0.0013 | 0.0009 | 0.003 | 0.0008 | 0.0005 | 0.0006 | 0.0003 | 0.001 | 0.0042 | 0.0003 | 0.0008 | 0.0009 | 0.002 | 0.0015 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 242.4727 | 199.8361 | 177.13 | 166.1377 | 191.0136 | 213.1965 | 214.7691 | 180.2181 | 186.9231 | 210.7988 |
Translational Efficiency | 0.5243 | 0.5609 | 0.5445 | 0.5809 | 0.5445 | 0.5484 | 0.501 | 0.5551 | 0.5222 | 0.4758 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
216 | 1135 | 1431 | 1215 | 2104 | 2034 | 242 | 1453 | 2320 | 3169 | 1673 | 2668 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1630.14 | 1387.69 | 1924.71 | 1797.87 | 1391.55 | 1465.57 | 2129.16 | 1733.89 | 1413.76 | 1437.68 | 1954.28 | 1763.03 |
Standard Deviation | 239.73 | 250.76 | 300.56 | 331.79 | 214.05 | 231.75 | 272.95 | 320.59 | 227.40 | 241.64 | 305.32 | 327.29 |
Intensity Change Log 2 | — | -0.232311 | 0.239645 | 0.141293 | — | 0.074769 | 0.613592 | 0.317320 | — | -0.082744 | 0.423944 | 0.225032 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.004605 | 0.003841 | 0.000911 | 0.001673 | 0.001115 | 0.002573 | 0.000114 | 0.001266 | 0.001440 | 0.003027 | 0.000796 | 0.001451 |
Bud Neck | 0.002470 | 0.000623 | 0.001143 | 0.001433 | 0.000099 | 0.000145 | 0.000652 | 0.001033 | 0.000319 | 0.000316 | 0.001072 | 0.001215 |
Bud Site | 0.016985 | 0.006375 | 0.002153 | 0.007321 | 0.001468 | 0.002055 | 0.000479 | 0.003442 | 0.002913 | 0.003602 | 0.001911 | 0.005208 |
Cell Periphery | 0.020355 | 0.003285 | 0.000903 | 0.008710 | 0.001934 | 0.002587 | 0.003803 | 0.005427 | 0.003649 | 0.002837 | 0.001322 | 0.006922 |
Cytoplasm | 0.004526 | 0.002507 | 0.012063 | 0.002162 | 0.001621 | 0.002255 | 0.000024 | 0.001366 | 0.001892 | 0.002345 | 0.010321 | 0.001729 |
Cytoplasmic Foci | 0.003758 | 0.009455 | 0.002764 | 0.001375 | 0.008019 | 0.004203 | 0.000022 | 0.001746 | 0.007622 | 0.006084 | 0.002367 | 0.001577 |
Eisosomes | 0.000349 | 0.000280 | 0.000020 | 0.000265 | 0.000218 | 0.000287 | 0.000268 | 0.000165 | 0.000230 | 0.000284 | 0.000056 | 0.000211 |
Endoplasmic Reticulum | 0.833784* | 0.919762* | 0.828394* | 0.918998* | 0.950561* | 0.954728* | 0.982113* | 0.946997* | 0.939689* | 0.942204* | 0.850629* | 0.934246* |
Endosome | 0.014785 | 0.015877 | 0.023506 | 0.010241 | 0.010062 | 0.008151 | 0.002986 | 0.007790 | 0.010502 | 0.010918 | 0.020537 | 0.008906 |
Golgi | 0.009847 | 0.013494 | 0.005503 | 0.009094 | 0.009900 | 0.006818 | 0.003208 | 0.004978 | 0.009895 | 0.009209 | 0.005171 | 0.006853 |
Lipid Particles | 0.045781 | 0.013451 | 0.015186 | 0.017186 | 0.007623 | 0.009612 | 0.002584 | 0.012369 | 0.011175 | 0.010987 | 0.013363 | 0.014563 |
Mitochondria | 0.003876 | 0.000790 | 0.000254 | 0.002349 | 0.001976 | 0.000697 | 0.000039 | 0.001006 | 0.002153 | 0.000731 | 0.000223 | 0.001617 |
Mitotic Spindle | 0.001028 | 0.000156 | 0.001082 | 0.001760 | 0.000067 | 0.000089 | 0.000009 | 0.000277 | 0.000157 | 0.000113 | 0.000926 | 0.000952 |
None | 0.000098 | 0.001168 | 0.000956 | 0.000284 | 0.000869 | 0.001529 | 0.000008 | 0.000561 | 0.000797 | 0.001400 | 0.000819 | 0.000435 |
Nuclear Periphery | 0.002292 | 0.000176 | 0.023394 | 0.000680 | 0.000117 | 0.000092 | 0.000683 | 0.000575 | 0.000319 | 0.000122 | 0.020108 | 0.000623 |
Nuclear Periphery Foci | 0.008952 | 0.003876 | 0.070823 | 0.003897 | 0.000968 | 0.000693 | 0.000796 | 0.002894 | 0.001711 | 0.001833 | 0.060693 | 0.003351 |
Nucleolus | 0.000011 | 0.000006 | 0.000006 | 0.000012 | 0.000004 | 0.000003 | 0.000001 | 0.000011 | 0.000005 | 0.000004 | 0.000005 | 0.000011 |
Nucleus | 0.000236 | 0.000062 | 0.000787 | 0.000132 | 0.000046 | 0.000264 | 0.000009 | 0.000120 | 0.000064 | 0.000192 | 0.000675 | 0.000125 |
Peroxisomes | 0.000177 | 0.001180 | 0.000033 | 0.000364 | 0.001521 | 0.001872 | 0.000003 | 0.000353 | 0.001396 | 0.001624 | 0.000028 | 0.000358 |
Vacuole | 0.020578 | 0.002416 | 0.009262 | 0.007623 | 0.000906 | 0.000788 | 0.001975 | 0.005517 | 0.002738 | 0.001371 | 0.008208 | 0.006476 |
Vacuole Periphery | 0.005508 | 0.001218 | 0.000857 | 0.004443 | 0.000904 | 0.000559 | 0.000224 | 0.002107 | 0.001332 | 0.000795 | 0.000766 | 0.003171 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 0.31 | 1.57 | 1.24 | 2.30 | -1.50 | -2.31 | 4.91 | -0.29 | 1.66 | -2.27 | -2.84 | 1.49 | -0.11 | 2.63 | -1.46 |
Bud Neck | 2.14 | 1.56 | 1.18 | -2.52 | -0.96 | -1.44 | -1.54 | -3.01 | -2.86 | -0.91 | 0.36 | -4.49 | -3.75 | -4.08 | -0.59 |
Bud Site | 2.02 | 2.75 | 1.73 | -0.75 | -3.27 | -1.04 | 2.20 | -2.80 | -1.72 | -3.79 | -0.65 | 1.31 | -2.45 | -1.92 | -3.60 |
Cell Periphery | 3.51 | 4.02 | 2.33 | -4.57 | -7.12 | -1.46 | -1.08 | -5.98 | -4.10 | -0.92 | 1.39 | 4.17 | -4.38 | -6.18 | -8.73 |
Cytoplasm | 1.14 | -3.54 | 1.25 | 0.42 | 6.91 | -1.88 | 7.32 | 0.49 | 2.02 | -4.46 | -1.32 | -7.24 | 0.27 | 1.47 | 7.27 |
Cytoplasmic Foci | -2.97 | 0.82 | 1.95 | 6.66 | 2.17 | 3.51 | 8.84 | 6.25 | 3.30 | -4.22 | 1.56 | 5.27 | 6.94 | 7.11 | 1.44 |
Eisosomes | 0.60 | 3.13 | 0.70 | 0.19 | -4.55 | -1.33 | -0.48 | 1.23 | 2.52 | 1.01 | -1.22 | 5.00 | 0.44 | 1.73 | -4.84 |
Endoplasmic Reticulum | -4.27 | 0.33 | -4.11 | 0.35 | -9.97 | -0.96 | -5.83 | 0.93 | 1.73 | 6.03 | -0.64 | 12.79 | 1.38 | 2.13 | -11.83 |
Endosome | -0.33 | -2.98 | 1.55 | 3.63 | 8.14 | 2.28 | 8.66 | 2.75 | 0.37 | -6.24 | -0.49 | -8.03 | 1.88 | 2.45 | 9.35 |
Golgi | -0.99 | 1.23 | 0.18 | 2.07 | -2.23 | 2.58 | 6.06 | 4.38 | 1.78 | -1.94 | 0.59 | 4.24 | 2.61 | 2.19 | -1.66 |
Lipid Particles | 3.31 | 3.05 | 2.88 | -1.66 | -0.61 | -1.41 | 3.22 | -3.23 | -1.62 | -5.19 | 0.14 | -1.29 | -2.19 | -2.57 | -0.45 |
Mitochondria | 0.91 | 1.06 | 0.43 | -1.56 | -2.14 | 1.84 | 2.87 | 1.22 | -0.67 | -2.26 | 2.03 | 2.78 | 0.62 | -1.71 | -2.76 |
Mitotic Spindle | 1.81 | -0.24 | -0.74 | -1.82 | -0.67 | -0.63 | 3.02 | -1.25 | -1.12 | -1.61 | 0.77 | -3.62 | -1.92 | -2.04 | 0.07 |
None | -2.01 | -2.67 | -2.17 | 1.66 | 2.07 | -1.43 | 3.85 | 1.02 | 2.15 | -2.68 | -1.59 | -0.08 | 1.55 | 2.84 | 1.31 |
Nuclear Periphery | 1.22 | -7.74 | 0.92 | -5.76 | 10.87 | 1.21 | -4.14 | -2.47 | -2.61 | 0.43 | 1.20 | -10.97 | -1.58 | -4.63 | 10.83 |
Nuclear Periphery Foci | 1.56 | -12.06 | 1.58 | -0.06 | 16.42 | 0.87 | 0.44 | -2.77 | -3.41 | -3.11 | -0.20 | -16.86 | -2.66 | -2.52 | 16.31 |
Nucleolus | 1.26 | 1.16 | 0.06 | -1.77 | -1.75 | 0.27 | 1.88 | -1.77 | -2.01 | -3.03 | 0.25 | -0.57 | -2.34 | -2.79 | -2.28 |
Nucleus | 1.78 | -2.29 | 0.92 | -2.18 | 3.11 | -0.98 | 6.47 | -1.75 | 0.63 | -2.51 | -0.87 | -3.53 | -2.15 | 0.39 | 3.10 |
Peroxisomes | -3.08 | 2.57 | -1.22 | 2.33 | -2.30 | -0.74 | 4.96 | 3.64 | 4.04 | -3.67 | -0.60 | 4.91 | 3.58 | 4.63 | -3.90 |
Vacuole | 4.06 | 2.39 | 2.81 | -4.38 | 1.17 | 0.45 | -1.59 | -4.83 | -4.87 | -3.16 | 2.66 | -5.25 | -4.62 | -6.90 | 1.54 |
Vacuole Periphery | 1.47 | 1.59 | 0.33 | -2.94 | -3.25 | 1.89 | 4.96 | -2.31 | -2.95 | -3.67 | 1.71 | 1.71 | -2.91 | -4.13 | -4.12 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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